Ochrobactrum angelica root growth-promoting bacterium and application thereof
By using a microbial agent prepared from Ochrobactrum sp. OS_1_202409, the problems of root development and disease in Angelica cultivation were solved, resulting in increased biomass and yield, improved soil microecology, and meeting the needs of green agricultural development.
Patent Information
- Application Number
- CN202511855271.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-10
- Publication Date
- 2026-02-10
AI Technical Summary
Existing technologies lack specialized functional microbial agents for the soil and cultivation methods of Angelica sinensis' native producing areas, making it difficult to effectively promote root development, improve nutrient utilization, and reduce diseases. Furthermore, the use of chemical pesticides leads to soil microecological imbalance and increased pesticide resistance.
Ochrobactrum sp. OS_1_202409 was used as a rhizosphere growth-promoting bacterium. It was cultured in LB liquid medium and prepared into a bacterial agent. The agent was then used to treat plants at different developmental stages, including pre-transplanting seedling soaking and root irrigation during the seedling emergence period, to promote plant growth and resist bolting.
It significantly increases the biomass and yield of Angelica sinensis, reduces bolting rate, improves the rhizosphere microecological environment, and reduces environmental pollution caused by the use of chemical pesticides, which meets the requirements of green agricultural development.
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Figure CN121495791A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of microbial technology, specifically to a highly adaptable rhizosphere growth-promoting bacterium of the genus *Ailuropoda* and its applications. Background Technology
[0002] Angelica sinensis ( Angelica sinensis Angelica sinensis (Oliv.) Diels is a perennial herb belonging to the genus Angelica in the family Apiaceae. It is one of the major traditional Chinese medicinal herbs, known for its blood-nourishing, blood-activating, menstrual-regulating, pain-relieving, and bowel-regulating effects. It is widely used in traditional Chinese medicine preparations related to gynecology, blood deficiency, and cardiovascular diseases. Angelica sinensis also has edible and health-promoting functions, often used as a seasoning spice or ingredient in medicinal cuisine. With increasing health awareness and the development of the traditional Chinese medicine industry, in addition to traditional processed medicinal herbs and prepared medicines, various value-added products using Angelica sinensis as a raw material or extract have emerged in the market, such as Angelica sinensis essential oil, oral liquids, health foods, and related daily chemical products. The demand for high-quality Angelica sinensis raw materials is increasing daily.
[0003] High-quality angelica root exhibits distinct "regional" characteristics, with its intrinsic quality closely related to the ecological environment of the production area, the variety's germplasm, and cultivation management practices. Currently, angelica root in major producing areas mostly adopts a "three-year cycle" cultivation method: the first year involves seed cultivation, seedbed development, and overwintering storage; the second year involves transplanting seedlings to the field for medicinal root production; and the third year involves reserving some plants for seed. Meanwhile, production is primarily based on scattered planting by individual farmers, and an integrated supply system with unified varieties, sources, and standardized cultivation management has not yet been established. Limited by local arable land, high multiple cropping index, and frequent continuous cropping, soil problems in the fields are becoming increasingly prominent, with high frequency of pests and diseases, severe bolting, and some plots experiencing weak plant growth, deformed root systems, reduced yields, or even crop failure, making it difficult to guarantee the stability of yield and quality.
[0004] In terms of fertilization and plant protection, there is currently a lack of fertilizers and pest control agents specifically developed for Angelica sinensis based on its biological characteristics and the ecological environment of its authentic producing areas. Production mainly relies on broad-spectrum chemical fertilizers, organic fertilizers, and conventional agricultural inputs such as chemical fungicides and insecticides. While these inputs can alleviate nutrient deficiencies or pest damage to some extent, they present several problems: First, the formulations lack specificity, making it difficult to meet Angelica sinensis's balanced needs for nitrogen, phosphorus, potassium, and micronutrients, as well as its special requirements for root enlargement and accumulation of active ingredients. Second, long-term use of chemical pesticides can easily lead to soil microecological imbalance, increased pesticide resistance, and pesticide residues, which are detrimental to the safety of Angelica sinensis and the requirements of green production.
[0005] In recent years, utilizing rhizosphere functional microorganisms to improve soil microecology, promote crop nutrient absorption and growth, and enhance agricultural product quality through rhizosphere environment regulation has become an important direction for green agricultural development and high-quality, high-efficiency production of traditional Chinese medicinal materials. Existing reports indicate that some growth-promoting rhizosphere bacteria, phosphate-solubilizing bacteria, nitrogen-fixing bacteria, and antagonistic bacteria can promote plant root development, improve nutrient utilization, and reduce disease occurrence through pathways such as producing plant hormones, solubilizing phosphorus, fixing nitrogen, secreting organic acids, and secreting antagonistic substances.
[0006] However, the current supply of functional strains and specialized microbial agents for the quality regulation of Angelica sinensis, especially for the root exudates-rhizosphere microorganisms in the authentic producing areas of Angelica sinensis, is still relatively weak. Most existing microbial fertilizers and biocontrol agents are developed for grain crops, vegetables or fruit trees, and there is a lack of specialized functional strains and supporting application techniques adapted to the growth habits and authentic ecological conditions of Angelica sinensis.
[0007] In summary, the existing technology lacks a specific functional microbial agent for Angelica sinensis that has a clear effect on promoting growth and improving quality, and can stably improve the rhizosphere microecological environment, tailored to the soil and cultivation methods of the authentic producing areas of Angelica sinensis. Summary of the Invention
[0008] The purpose of this invention is to provide a rhizosphere growth-promoting bacterium of Angelica sinensis with significant anti-bolting and root-strengthening properties and its application.
[0009] The rhizosphere growth-promoting bacteria of Angelica sinensis claimed in this invention are Aristolochic acid bacilli ( Ochrobactrum sp. The strain OS_1_202409 was deposited at the China Center for Type Culture Collection (CCTCC) on November 20, 2025, with accession number CCTCC M 20252607.
[0010] The *Aleucobacterium pachycarpa* described in this invention ( Ochrobactrum sp. OS_1_202409 has been identified as having significant root-strengthening and bolting-resistant functions.
[0011] A growth-promoting inoculum of *Bacillus cereus* rhizosphere bacteria, obtained by the following method: Paleobacterium Ochrobactrum sp. OS_1_202409 was cultured in LB liquid medium under the following conditions: 25-30°C, 180-200 rpm for 24-48 hours; the *Aureobacterium* species... Ochrobactrum sp. The concentration of OS_1_202409 in the bacterial agent is 1×10⁻⁶. 8 ~1×10 11 cfu / mL.
[0012] As a preferred option, the above-mentioned LB liquid culture medium formula is as follows: 10-20g tryptone, 5-10g yeast powder, 10-20g NaCl, add deionized water to make up to 1000-2000mL, adjust the pH to 6.8-7.2, and sterilize at 121℃ for 20-30 min for later use.
[0013] This invention claims protection for *Aureobacterium pachyphyllum* ( Ochrobactrum sp. The use of OS_1_202409) or its bacterial suspension or culture medium or its fermentation product or an agent containing it in any of the following A1)-A3): A1) Produces indoleacetic acid (IAA); A2) Promotes the growth of Angelica sinensis; A3) Resisting bolting of Angelica sinensis; The active ingredient of the product for which protection is sought in this invention is *Ailuropoda spp.* Ochrobactrum sp. OS_1_202409) or its bacterial suspension or culture medium or its fermentation product or bacterial agent containing it; The product may be used for all or some of the following purposes: B1) Promotes the growth of the entire Angelica sinensis plant; B2) Resisting bolting of Angelica sinensis; In any of the above-described applications or products, the product may be a microbial fertilizer.
[0014] This invention seeks protection for a method for promoting plant growth.
[0015] The method for promoting plant growth claimed in this invention includes using the above-mentioned *Aureobacterium tumefaciens* (…). Ochrobactrum sp. OS_1_202409) or its bacterial suspension or culture medium or its fermentation product or an inoculum containing it, are used to treat plants, including Angelica sinensis.
[0016] The specific methods for treating plants may include soaking seedlings before transplanting, applying the solution to the seedling holes, irrigating the roots during the seedling emergence period, and spraying the above-ground parts.
[0017] The resistance to bolting of Angelica sinensis described above is manifested in all or part of the following: C1) Production of indoleacetic acid (IAA) C2) Promoting plant growth at different developmental stages of the plant. C3) Delaying flowering at different developmental stages of the plant.
[0018] The above-mentioned promotion of plant growth manifests itself in all or part of the following: D1) Promotes an increase in plant height / crown width at different developmental stages of the plant; D2) Promotes an increase in aboveground biomass (aboveground dry weight) and / or underground biomass (root weight) of the plant at different developmental stages; D3) Promotes increased yield of the plant.
[0019] In any of the above-described applications, products, or methods, the bacterial suspension, culture medium, fermentation product, or bacterial agent contains, in addition to the *Bacillus cereus* as an active ingredient, the *Bacillus* (*Bacillus*). Ochrobactrum sp In addition to .OS_1_202409, it also contains excipients, which can be selected as needed.
[0020] Furthermore, the promotion of plant growth can be carried out in the main production areas of Angelica sinensis.
[0021] Furthermore, the main production areas are specifically in the northwest and southwest regions of my country (such as Gansu Province, specifically Minxian County, Dingxi City, Gansu Province). In any of the above-described applications, products, or methods, the different developmental stages are any of the following: transplanting period (0 days after emergence), seedling stage (25 days after emergence), early bolting outbreak period (90 days after emergence), autumn bolting period (120 days after emergence), and harvest period (180 days after emergence).
[0022] In one embodiment of the present invention, the bacterial agent is specifically a liquid bacterial agent, which is cultured in LB liquid medium to cultivate the *Ailuropoda spp.* (…). Ochrobactrum sp The culture conditions were obtained from .OS_1_202409; specifically, the culture conditions were: 30℃, 180r / min for 24-48 hours; the *Aureobacterium lancifolium* (…) Ochrobactrum sp The concentration of .OS_1_202409) in the liquid bacterial agent is specifically 1×10 8 -1×10 11 cfu / mL.
[0023] In any of the above-described applications, products, or methods, the plant may be an economic crop, including angelica.
[0024] The application of any of the products or methods described above in increasing plant height and / or increasing aboveground biomass and / or increasing underground biomass and / or increasing leaf area index and / or increasing 100-grain weight and / or increasing plant yield is also within the scope of protection of this invention.
[0025] The strain Ochrobactrum sp. OS_1_202409 of this invention was isolated from the rhizosphere soil of Angelica sinensis. This strain has anti-bolting and growth-promoting functions. In the absence of any other commercial reagents, the application of this agent in the field can significantly reduce the bolting rate of Angelica sinensis and increase the biomass of Angelica sinensis at different developmental stages compared with the negative control without the application of the agent. Moreover, the yield level is not significantly different from that of currently commercial reagents.
[0026] This invention provides highly efficient root-strengthening microbial germplasm resources for the main Angelica sinensis growing areas in Northwest and Southwest my country, which is conducive to promoting the green, healthy, and sustainable development of agriculture in my country. Utilizing this microorganism reduces the input of pesticides and fertilizers and mitigates the environmental pollution caused by excessive use of these substances, meeting the needs of sustainable green agriculture and the requirements of ecological agriculture in my country. It not only expands the germplasm resources of growth-promoting microorganisms for economic crops in my country but also provides a foundation for the research and development of highly efficient and stable functional microbial fertilizers and their application technologies. Attached Figure Description
[0027] Figure 1 For pale bacilli ( Ochrobactrum sp Colony morphology of .OS_1_202409 on LB solid medium.
[0028] Figure 2 For pale bacilli ( Ochrobactrum sp Qualitative analysis diagram of IAA production (.OS_1_202409).
[0029] Figure 3 For pale bacilli ( Ochrobactrum sp Comparison of potted plants after treatment with .OS_1_202409)
[0030] Figure 4 For pale bacilli ( Ochrobactrum sp Comparison of aboveground biomass in the field after treatment with .OS_1_202409.
[0031] Figure 5 For pale bacilli ( Ochrobactrum sp Comparison of medicinal roots from field experiments after treatment with .OS_1_202409. Detailed Implementation
[0032] The following examples are provided to better understand the present invention, but are not intended to limit the invention. Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Unless otherwise specified, the experimental materials used in the following examples were purchased from conventional biochemical reagent stores.
[0033] Example 1: Isolation, identification, and functional determination of bolting-resistant and growth-promoting bacterial strains Minxian County is one of the major angelica cultivation areas in my country, and it urgently needs to take effective protective measures to increase angelica yield and reduce bolting. This invention addresses the current state of angelica production by isolating a strain with growth-promoting and bolting-resistant functions from the rhizosphere soil of angelica seedlings.
[0034] 1. Experimental materials (1) Soil sample collection Several Angelica sinensis plants growing normally and without obvious pests or diseases were selected from the main producing areas of Angelica sinensis. Rhizosphere soil samples were collected at random points in the sample plot using the 5-point sampling method. Specifically, the Angelica sinensis plants were gently pulled up, loose soil was shaken off, and soil within an area of about 0-5 mm close to the root surface was collected with a sterile brush or sterile spoon, mixed well, and used as the Angelica sinensis rhizosphere soil sample.
[0035] (2) Preparation of culture medium 1) LB (Luria-Bertani Agar) solid medium Tryptone: 10.0 g, yeast extract: 5.0 g, NaCl: 10.0 g, agar: 20.0 g, deionized water: 1000 mL. Mix and dissolve all ingredients, adjust pH to 6.8–7.2, dispense into Erlenmeyer flasks or reagent bottles, and autoclave at 121 °C for 20 min. Liquid LB medium has the same formulation, but agar is omitted.
[0036] 2) Preparation of LB liquid medium containing L-tryptophan 10.0 g tryptone, 5.0 g yeast extract, 10.0 g sodium chloride (NaCl), 0.5 g L-tryptophan, and deionized water to a final volume of 1000 mL. Dissolve the above ingredients in approximately 800 mL of deionized water, and after complete dissolution, bring the volume to 1000 mL. Adjust the pH to 6.8–7.2. Aliquot into Erlenmeyer flasks or reagent bottles, autoclave at 121 °C for 20 min, and cool before use.
[0037] 2. Experimental Methods (1) Bacteria in the rhizosphere soil were extensively isolated, purified, and cultured using LB solid medium. After restoring the Angelica rhizosphere soil sample stored at 4℃ to room temperature, 5.0 g of soil sample was weighed and 45 mL of pre-sterilized physiological saline was added. The mixture was thoroughly shaken and mixed to prepare a 10⁻¹ soil suspension. This suspension was then serially diluted 10⁻¹ to 10⁻¹ times. 6 Soil suspensions with different dilutions.
[0038] Take 100 μL of each dilution of soil suspension and spread it evenly on the surface of LB agar plates. After air drying at room temperature, place the plates in an inverted incubator at 30℃ for 48 h. After incubation, on plates with fully grown and clearly distributed colonies, select representative single colonies based on colony morphology differences (including colony size, color, edge morphology, surface smoothness, and transparency) and inoculate them onto new LB agar plates for streak purification. Repeat streak isolation for 2-3 generations until a pure strain with consistent morphology and no contamination is obtained.
[0039] The obtained pure strain was activated and cultured in LB liquid medium. After the culture was completed, sterile glycerol with a final concentration of 20% to 30% was added, mixed well, and dispensed into sterile cryovials for long-term storage at -80 ℃.
[0040] The purified strains were subjected to morphological observation, including colony morphology characteristics on LB solid medium, such as... Figure 1 And cell morphology under an optical microscope.
[0041] Genomic DNA was extracted from the strain, and PCR amplification was performed using universal primers for the 16S rRNA gene. The amplified product was sequenced to obtain the 16S rRNA gene sequence of the strain. The obtained sequence was submitted to public databases such as NCBI for BLAST homology comparison. The gene sequence was determined as follows:
[0042] Based on the comparison results and phylogenetic analysis, this strain was identified as *Bacillus pallida*. Ochrobactrum sp .OS_1_202409). (2) Functional identification of the isolated and purified bacteria for producing indoleacetic acid (IAA). Salkowski colorimetric qualitative determination of IAA-producing strains: The purified strain Ochrobactrum sp. OS_1_202409 was inoculated into LB broth containing L-tryptophan and cultured with shaking at 30 ℃ and 180 r / min for 24 h. After culture, the bacterial culture was transferred to a centrifuge tube and centrifuged at 8000 r / min for 5 min, and the supernatant was collected. 1.0 mL of the supernatant was transferred to a 1.5 mL centrifuge tube, and an equal volume of Salkowski chromogenic reagent was added. The mixture was gently mixed and incubated at room temperature in the dark for 20–30 min. A blank control was prepared by mixing the supernatant of uninoculated LB broth (with an equal amount of L-tryptophan added and cultured under the same conditions) with an equal volume of Salkowski chromogenic reagent.
[0043] After standing and allowing the solution to develop color, observe the color change: A positive result for IAA is indicated by the solution changing from colorless or pale yellow to pink to red; no obvious color change indicates a negative result. Results are as follows: Figure 2 As shown, Ochrobactrum sp.OS_1_ 202409 The culture supernatant of the strain turned red after reacting with Salkowski chromogenic solution, while the blank control did not show any color, indicating that the strain can produce IAA.
[0044] against Pseudomonas aeruginosa ( Ochrobactrum sp The growth-promoting function of strain .OS_1_202409 in pot cultivation was verified, as shown in Table 1. Ochrobactrum sp. OS_1_202409 can significantly increase the crown width, number of stem divisions, plant height, and number of leaves of potted angelica.
[0045] Table 1 deal with Crown width (cm) Plant height (cm) Number of stem divisions (individuals) CK (no bacterial agent group) 4.13±0.72 4.34±1.94 1.17±0.41 SL (using microbial agents) 16.13±3.81 9.38±1.42 3.67±0.52
[0046] Example 2 Ochrobactrum sp. Preparation and field application of OS_1_202409 inoculant 1. Experimental Materials and Methods (1) Experimental materials 1) Source of strain The bacterial strain used in this embodiment is the one obtained in Example 1, which has growth-promoting and bolting-resistant functions. Ochrobactrum sp. OS_1_202409.
[0047] 2) Culture medium Liquid LB (Luria–Bertani) medium was used, with the following formula: 10.0 g tryptone, 5.0 g yeast extract, 10.0 g NaCl, and deionized water was added to bring the volume to 1000 mL. The pH was adjusted to 6.8–7.2, and the medium was sterilized at 121 °C for 20 min before use.
[0048] 3) Experimental site and test crops In the main production areas of Angelica sinensis, field sites with relatively consistent soil conditions and cultivation management were selected as experimental sites. The test crop was the locally conventionally cultivated Angelica sinensis variety. The previous crop was rapeseed, and the experimental sites were plots with multiple cropping or high multiple cropping index to enhance the experimental effect on resistance to bolting and growth promotion.
[0049] (2) Preparation of microbial agents 1) Activation and scale-up of bacterial strains Take out the stored product from the -80°C cryovial. Ochrobactrum sp. After rapid thawing at room temperature, a small amount of bacterial cells was picked up with a sterile inoculation loop and inoculated into liquid LB medium. The medium was then cultured with shaking at 37°C and 180 r / min until the turbidity reached the logarithmic growth phase. The optical density (OD) was measured at 600 nm using a spectrophotometer. 600 When OD 600 When the concentration reaches 0.8, the bacterial concentration is considered to have reached the predetermined level (approximately 10). 8 (Approximately CFU / mL) is used as the fermentation broth for preparing the bacterial agent.
[0050] 2) Collection and resuspension of bacterial cells to prepare bacterial inoculum Cultivate to OD 600 ≈0.8 Ochrobactrum sp. Pour the OS_1_202409 bacterial culture into a sterile centrifuge tube and centrifuge at 4 ℃ and 4000~8000 r / min for 5~10 min. Discard the supernatant and retain the bacterial precipitate.
[0051] Add an appropriate amount of sterile physiological saline or sterile water to the bacterial precipitate for resuspending. Gently shake or pipette to fully disperse the bacteria, and adjust the volume of the resuspended solution to maintain the final bacterial suspension concentration at approximately 10. 8 CFU / mL. In this example, the obtained Ochrobactrum sp. OS_1_202409 bacterial suspension was used as the stock solution for field trials.
[0052] (3) Field trial design and application methods 1) Experimental Design A randomized block design was used, with two treatments: a control group (CK) and a treatment group (OS), each with three replicates. CK: Not administered Ochrobactrum sp. OS_1_202409 bacterial agent, apply only an equal amount of water; OS: Application Ochrobactrum sp. OS_1_202409 bacterial agent.
[0053] Each plot has an area of 13.5 m². The row spacing and plant spacing are carried out according to the local conventional cultivation methods for Angelica sinensis. Other fertilizer and water management and pest and disease control are carried out in accordance with local conventional agricultural practices.
[0054] 2) Ochrobactrum sp. OS_1_202409 Inoculant Application Method In this embodiment, the method of "planting and mixing in the planting hole + root irrigation during the seedling stage" is used to apply the treatment twice: First application (mixing the soil around the planting hole during transplanting). When transplanting Angelica seedlings to the field, a certain amount of [unclear text - possibly a typo, should be] added to each planting hole beforehand. Ochrobactrum sp. OS_1_202409 bacterial agent working solution. Specifically: [The following text appears to be incomplete and requires further context: "using..."] Ochrobactrum sp. OS_1_202409 bacterial agent stock solution is the working solution, maintaining the viable bacteria count in the working solution at 10. 7 ~10 8 Within the CFU / mL range. When transplanting, add 5 mL per hole. Ochrobactrum sp. The OS_1_202409 bacterial agent working solution is evenly poured into the planting hole, and then the Angelica seedling is placed in the hole, covered with soil and gently pressed down to ensure that the bacterial solution is in full contact with the roots and the surrounding soil.
[0055] Second application (root drenching during seedling emergence) When the angelica seedlings have recovered from transplant shock, the plants emerge uniformly, and enter a stable growth period, a second [treatment / treatment] will be carried out. Ochrobactrum sp. OS_1_202409 bacterial agent application. Prepare according to the same dilution method as the first time. Ochrobactrum sp. OS_1_202409 bacterial inoculant working solution was applied by drenching near the root zone of the plant, with 5 mL applied to each hole. Ochrobactrum sp. OS_1_202409 bacterial agent working solution allows the bacterial solution to penetrate into the soil around the roots.
[0056] Throughout the experiment, the CK treatment group was given the same amount of clean water at the same time and location.
[0057] 3) Survey Indicators and Methods The following indicators were used to investigate and measure different growth stages of Angelica sinensis: Bolting sensitive period: Investigate the number of bolting plants in each plot and calculate the bolting rate (number of bolting plants / total number of plants × 100%). Harvest period: A certain number of Angelica sinensis plants were randomly dug up, and the fresh weight of the roots and the diameter of the rhizome of each plant were measured. The yield per unit area of each treatment plot was also calculated.
[0058] The significance of each indicator's data was tested using a t-test to evaluate... Ochrobactrum sp. The anti-bolting and yield-increasing effects of OS_1_202409 microbial agent.
[0059] 2. Experimental Results A field trial over one season showed that Ochrobactrum sp. When OS_1_202409 microbial agent is applied twice—once during transplanting and once during seedling emergence—it has a significant impact on the growth, development, and bolting of Angelica sinensis.
[0060] 1) Impact on output and marketability Results measured at harvest time indicate that Ochrobactrum sp. The yield per unit area of the OS_1_202409-treated plots was significantly higher than that of the CK-treated plots, with increased root weight per plant. Marketability was significantly improved.
[0061] 2) Effects on bolting The results of a survey on the bolting sensitivity period of Angelica sinensis showed that the number of bolting plants in the plot treated with Ochrobactrum sp. OS_1_202409 was significantly less than that in the control plot, with a bolting rate of 17.47%, compared to 38.13% in the control group. This indicates that the application of... Ochrobactrum sp. OS_1_202409 microbial agent can effectively delay the differentiation and bolting process of Angelica sinensis flower buds, inhibit bolting to a certain extent, and prolong the vegetative growth period.
[0062] deal with Thickness of the rhizome (cm) Fresh weight per plant (grams) Yield per square meter (kg / 13.5 m²) CK (without OS_1_202409 bacterial agent) 3.67±0.23 180.25±19.35 6.70±0.20 OS (application of OS_1_202409 bacterial agent) 5.50±0.58 278.00±59.30 10.83±0.59
[0063] In summary, this embodiment demonstrates that the present invention is effective in the field cultivation of Angelica sinensis. Ochrobactrum sp. OS_1_202409 microbial agent can significantly promote the growth of Angelica sinensis, reduce the bolting rate, and increase the yield of Angelica sinensis. It can be used to develop related products such as bio-fertilizers and has important practical application value.
[0064] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. *Aureobacterium lancifolium*, a rhizosphere growth-promoting bacterium of *Angelica sinensis*, is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20252607 and is classified as *Aureobacterium lancifolium*. Ochrobactrum sp. OS_1_202409.
2. A growth-promoting inoculum of *Bacillus cereus* rhizosphere bacteria, characterized in that, It is obtained through the following method: Ochrobactrum sp. OS_1_202409 was cultured in LB liquid medium under the following conditions: 25-30°C, 180-200 rpm for 24-48 hours. Ochrobactrum sp. The concentration of OS_1_202409 in the bacterial agent is 1×10⁻⁶. 8 ~1×10 11 cfu / mL.
3. The *Ailuropoda* spp. rhizosphere growth-promoting bacterial agent for *Angelica sinensis* according to claim 2, characterized in that, The LB liquid culture medium formula is as follows: 10-20g tryptone, 5-10g yeast powder, 10-20g NaCl, add deionized water to make up to 1000-2000mL, adjust the pH to 6.8-7.2, and sterilize at 121℃ for 20-30 min for later use.
4. The application of the Angelica rhizosphere growth-promoting bacteria as described in claim 1, a suspension of the bacteria, a culture medium of the bacteria, a fermentation product of the bacteria, or an inoculum containing the bacteria in preventing Angelica bolting.
5. The application of the Angelica sinensis rhizosphere growth-promoting bacteria, its suspension, its culture medium, its fermentation product, or its inoculum containing the bacteria as described in claim 1 in promoting the growth of Angelica sinensis.
6. The application according to claim 4 or 5, wherein the rhizosphere growth-promoting bacteria of Angelica sinensis, a suspension of the bacteria, a culture medium of the bacteria, or a fermentation product of the bacteria, or an inoculum containing the bacteria, promote the production of indoleacetic acid in Angelica sinensis.
7. A microbial agent for promoting the growth of Angelica sinensis, characterized in that, The bacterial agent includes the *Alanium* as described in claim 1. Ochrobactrum sp. OS_1_202409X, a suspension of the bacterium, a culture medium of the bacterium, or a fermentation product of the bacterium.
8. The application of the microbial agent according to claim 7 in preventing bolting of Angelica sinensis and promoting its growth.
9. The application according to claim 8, characterized in that, The main ways to promote the growth of Angelica sinensis are: It promotes an increase in plant height / crown width of the Angelica sinensis at different developmental stages; It promotes the increase of aboveground biomass and / or underground biomass (root weight) of Angelica sinensis at different developmental stages.
10. A method for preventing bolting and promoting the growth of Angelica sinensis, characterized in that, Paleobacterium Ochrobactrum sp. OS_1_202409 The suspension of the bacterium, the culture medium of the bacterium, or the fermentation product of the bacterium or an inoculum containing the bacterium are used to treat Angelica sinensis. The methods of treating Angelica sinensis include soaking the seedlings before transplanting, applying the solution to the seedling holes, irrigating the roots during the seedling stage, or spraying the above-ground parts.