Cryopreservation device

Abstract

The invention relates to the technical field of biological sample cryopreservation, in particular to a cryopreservation device which comprises a cryopreservation tube assembly, a storage container and a tube taking assembly, the cryopreservation tube assembly comprises a tube cap, a tube body and a bearing part, the tube cap is detachably connected with the tube body, the bearing part is connected with the tube cap, and the bearing part is provided with a bearing face used for bearing a target sample; the bearing surface is a curved surface and can extend into the tube body, so that a target sample is placed in the tube body; the storage container is provided with a placing position, and the pipe body can be limited at the placing position; the storage container is used for being immersed in a freezing medium, and the freezing medium can flow into the pipe body; the pipe taking assembly is provided with a first station and a second station, and at the first station, the pipe taking assembly can be connected with a pipe cap so as to separate the pipe cap from a pipe body or connect the pipe cap with the pipe body; and at the second station, the pipe taking assembly can be separated from the pipe cap. In the operation process, an operator does not need to directly contact the cryopreservation tube assembly with hands, so that the working efficiency and the operation safety are improved.

Description

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of biological sample cryopreservation, and particularly to a cryopreservation device. BACKGROUND

[0002] In the field of low-temperature preservation of biological samples such as gametes, zygotes or embryos, the tool used to cryopreserve the target sample is usually a straw or a cryopreservation tube. During the clinical operation of freezing or thawing, the operator needs to fix the straw or the sleeve of the cryopreservation tube with one hand and rotate or pull out the carrier with the other hand. Such operation is not only inefficient and prone to errors, but also has safety hazards. SUMMARY

[0003] The present disclosure aims to provide a cryopreservation device to improve work efficiency and operation safety.

[0004] To achieve the above purpose, the present disclosure provides a cryopreservation device, comprising:

[0005] a cryopreservation tube assembly, the cryopreservation tube assembly comprising a tube cap, a tube body and a carrier, the tube cap being detachably connected with the tube body, the carrier being connected with the tube cap, the carrier having a carrier surface for carrying a target sample, the carrier surface being a curved surface, the carrier surface being capable of extending into the tube body to place the target sample in the tube body;

[0006] a storage container, the storage container being provided with a placement position, the tube body being capable of being limited in the placement position; the storage container being used for being immersed in a freezing medium, the freezing medium being capable of flowing into the tube body; and

[0007] a tube taking assembly, the tube taking assembly having a first station and a second station, in the first station, the tube taking assembly is capable of being connected with the tube cap to separate the tube cap from the tube body or connect the tube cap with the tube body; in the second station, the tube taking assembly is capable of being separated from the tube cap.

[0008] In an embodiment of the present disclosure, an inner wall of the tube body is provided with a communication groove, the communication groove has a first end and a second end along an extension direction of the communication groove, the first end of the communication groove penetrates through an end surface of the tube body facing the tube cap, and the second end of the communication groove is located in the tube body, the communication groove is configured to make the speed of the freezing medium flowing from the first end to the second end greater than the speed of the freezing medium flowing from the second end to the first end.

[0009] In an embodiment of the present disclosure, the width of the first end of the communication groove is greater than the width of the second end of the communication groove, or the communication groove is a Tesla valve groove.

[0010] In one embodiment of the present disclosure, the shape of the curved surface is arc-shaped, V-shaped, wavy, trapezoidal or concave.

[0011] In one embodiment of the present disclosure, the bearing surface is provided with a through hole for positioning the target sample.

[0012] In one embodiment of the present disclosure, the through hole is a circular hole with a diameter of 0.2-0.7 mm.

[0013] In one embodiment of the present disclosure, the cap is threadedly connected to the tube body, the tube body is provided with a limiting portion, the storage container is provided with a limiting matching portion, and the limiting portion and the limiting matching portion are matched to limit the rotation of the tube body around its own axis.

[0014] In one embodiment of the present disclosure, the tube taking assembly comprises a shell and a push rod, the shell is a hollow structure with open ends, one of the shell and the cap is provided with an embedding portion, and the other is provided with an embedding matching portion, at least one of the embedding portion and the embedding matching portion is elastically deformable to limit the embedding portion in the embedding matching portion;

[0015] The push rod is slidably arranged in the shell, in the first working position, the embedding portion is limited in the embedding matching portion to fix the shell and the cap; in the second working position, the push rod is movable relative to the shell to make the end of the push rod close to the embedding portion abut against the cap and make the embedding portion disengage from the embedding matching portion.

[0016] In one embodiment of the present disclosure, the embedding portion is provided with a clamping portion, the embedding matching portion is provided with a clamping matching portion, and the clamping matching portion is capable of cooperating with the clamping portion to fix the shell and the cap.

[0017] In one embodiment of the present disclosure, the embedding portion is arranged at one end of the shell and is elastically deformable, the embedding matching portion is arranged at the end of the cap away from the tube body, the embedding matching portion is a receiving groove, and the clamping matching portion is arranged on the groove wall of the receiving groove; one of the clamping portion and the clamping matching portion is a clamping protrusion, and the other is a clamping groove, in the first working position, the clamping protrusion is limited in the clamping groove, and in the second working position, the clamping protrusion is separated from the clamping groove.

[0018] In one embodiment of the present disclosure, the cross-sectional profile of the groove wall of the receiving groove is a concave polygon; and / or, the surfaces of the clamping protrusion and the clamping groove in contact with each other are spherical cap curved surfaces or ellipsoidal surfaces.

[0019] In one embodiment of the present disclosure, the tube taking assembly further comprises a reset member; the push rod is provided with a first stop portion, the shell is provided with a second stop portion, and the reset member is located between the first stop portion and the second stop portion, and the reset member can switch the push rod from the second station to the first station.

[0020] In one embodiment of the present disclosure, the tube taking assembly further comprises a hollow limiting column, which is installed in the shell, and the push rod is arranged in the hollow limiting column, and the second stop portion is an end face of the hollow limiting column facing the reset member.

[0021] In one embodiment of the present disclosure, the shell is provided with a limiting groove, and the extending direction of the limiting groove is consistent with the length direction of the shell; the push rod is provided with a protruding portion, and the protruding portion can reciprocate along the extending direction of the limiting groove, and in the first station, the protruding portion can abut against one end of the limiting groove away from the cap.

[0022] In one embodiment of the present disclosure, the cryopreservation tube assembly further comprises an identification member, which is installed on the cap, and the identification member has an identification face, which faces away from the tube body, and the identification face is provided with sample information.

[0023] In one embodiment of the present disclosure, the identification member is provided with an insertion portion, which is provided with a positioning protruding portion, and the cap is provided with a insertion hole, and the insertion portion can be inserted into the insertion hole, and the positioning protruding portion can abut against the end face of the cap facing the tube body.

[0024] In one embodiment of the present disclosure, the cryopreservation tube assembly further comprises a counterweight, which is installed on one end of the tube body away from the cap, and the counterweight is provided with a discharge hole.

[0025] In one embodiment of the present disclosure, at least one of the counterweight and the tube body is provided with a protruding portion, which is used to limit the axial movement of the counterweight along the tube body.

[0026] In one embodiment of the present disclosure, the protruding portion is arranged on the inner wall of one end of the tube body away from the cap, and the protruding portion abuts against the surface of the counterweight away from the cap.

[0027] In one embodiment of the present disclosure, the counterweight is in interference fit with the tube body through the protruding portion; the protruding portion is arranged on the circumferential side wall of the counterweight, and / or the protruding portion is arranged on the inner wall of one end of the tube body away from the cap.

[0028] In one embodiment of the present disclosure, the gap is provided between the cap and the tube body for the frozen medium to enter the tube body.

[0029] In one embodiment of the present disclosure, the storage container comprises a box body and a box cover, and the box cover is detachably covered on the box body.

[0030] The placing positions are arranged in the box body, and the placing positions are arranged in rows and columns in the box body.

[0031] In one embodiment of the present disclosure, the box cover is provided with a flow port.

[0032] In one embodiment of the present disclosure, the carrier comprises a rod portion and a plate portion, one end of the rod portion is connected with the cap, the other end of the rod portion is connected with the plate portion, and the bearing surface is the plate surface of the plate portion.

[0033] In one embodiment of the present disclosure, the thickness of the plate portion is 0.05-0.25mm.

[0034] The present disclosure has the following beneficial effects:

[0035] (1) The cryopreservation device provided by the present disclosure can place the tube body in the placing position of the storage container, then immerse the storage container and the tube body in the frozen medium, and the frozen medium flows into the tube body. The tube body is connected with the cap at the first station, then the target sample is placed on the bearing surface of the carrier for freezing, and then the cap is connected to the tube body by the tube body, so that the target sample is immersed in the frozen medium. At this time, the tube body is switched to the second station, so that the tube body is separated from the cap, thereby completing the freezing operation. When the target sample needs to be taken out, the cap can be connected with the tube body at the first station by using the tube body, so as to separate the cap from the tube body, thereby taking out the target sample from the tube body, and then thawing the target sample. In the operation process, the operator does not need to directly contact the cryopreservation tube assembly with his hands, and the cooperation of the tube body and the cap and the cooperation of the storage container and the tube body can realize the connection or separation of the cap and the tube body and the transfer of the cryopreservation tube assembly, thereby improving the work efficiency and the operation safety. At the same time, since the bearing surface is a curved surface, the target sample is placed on the curved surface, which can increase the contact area between the target sample and the bearing surface, thereby better overcoming the influence of gravity, reducing the risk of sliding or losing the target sample, and making the target sample more stable and reliable during storage and transfer.

[0036] (2) The freezing device provided by the disclosure has a smaller volume and a thinner thickness, and can cross the ice crystal point at a very fast speed when freezing, thereby avoiding the influence of ice crystals on the target sample and making the target sample safer.

[0037] (3) The freezing device provided by the disclosure can balance the pressure inside and outside the pipe body and release gas, so that the pipe body will not be damaged or exploded due to the rapid expansion of nitrogen after being taken out of the freezing medium. In addition, after the pipe body is taken out daily, the freezing medium can be backfilled through the communication groove when the pipe body needs to be put back into the freezing medium, so as to ensure the safety of the sample.

[0038] (4) The freezing device provided by the disclosure has an identification member with an identification surface facing away from the pipe body on the side of the pipe cap, and the identification surface is provided with sample information, so that automatic and fast storage and identification of the sample information can be realized. Meanwhile, the multiple placement positions are arranged in rows and columns in the box body, the box body is provided with a mark, has a position mark and management function, and can realize one-to-one traceability management of the sample. BRIEF DESCRIPTION OF DRAWINGS

[0039] In order to more clearly illustrate the specific embodiments of the disclosure or the technical solutions in the prior art, the drawings needed in the specific embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are some embodiments of the disclosure, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0040] Figure 1 Structure diagram of the freezing tube assembly in the freezing device provided by the embodiment of the disclosure;

[0041] Figure 2 Structure diagram of the pipe body in the freezing tube assembly in the embodiment of the disclosure;

[0042] Figure 3 Structure diagram of the pipe body in the freezing tube assembly in the embodiment of the disclosure; Figure 2

[0043] Figure 4 Another structure diagram of the communication groove in the embodiment of the disclosure;

[0044] Figure 5 Partial structure diagram of the freezing tube assembly in the freezing device provided by the embodiment of the disclosure;

[0045] Figure 6 Top view of the freezing tube assembly in the freezing device provided by the embodiment of the disclosure;​

[0046] Figure 7A Structure diagram of the cap and the carrier in the cryopreservation tube assembly in the embodiment of the present disclosure;

[0047] Figure 7B Another structure diagram of the cap and the carrier in the cryopreservation tube assembly in the embodiment of the present disclosure;

[0048] Figure 8 Structure diagram of the identification member in the cryopreservation tube assembly in the embodiment of the present disclosure;

[0049] Figure 9 Structure diagram of the cryopreservation tube assembly in the cryopreservation device provided by the embodiment of the present disclosure from another perspective;

[0050] Figure 10 Structure diagram of the tube taking assembly in the cryopreservation device provided by the embodiment of the present disclosure; Figure 9 Enlarged view of II in the structure diagram;

[0051] Figure 11 Structure diagram of the tube taking assembly in the cryopreservation device provided by the embodiment of the present disclosure;

[0052] Figure 12 Enlarged view of III in the structure diagram; Figure 11

[0053] Side view of the tube taking assembly in the cryopreservation device provided by the embodiment of the present disclosure; Figure 13

[0054] Enlarged view of IV in the structure diagram; Figure 14 Figure 13 Structure diagram of the tube taking assembly in the embodiment of the present disclosure (the shell is not shown);

[0055] Figure 15 Figure 14 Enlarged view of V in the structure diagram;

[0056] Figure 16 Structure diagram of the tube taking assembly in the embodiment of the present disclosure (the shell is not shown);

[0057] Figure 17 Structure diagram of the cryopreservation device provided by the embodiment of the present disclosure (the tube taking assembly is not shown);

[0058] Figure 18 Exploded view of the cryopreservation device provided by the embodiment of the present disclosure (the tube taking assembly is not shown);

[0059] Figure 19 Structure diagram of the box body in the storage container in the embodiment of the present disclosure;

[0060] Figure 20 Top view of the box body in the storage container in the embodiment of the present disclosure. ​​

[0061] The reference signs are explained as follows:

[0062] 1-frozen tube assembly; 10-gap; 11-tube cap; 111-receiving groove; 112-clamping groove; 12-tube body; 121-communication groove; 1211-first end; 1212-second end; 122-limiting part; 123-protruding part; 13-carrier; 131-rod part; 132-plate part; 132A-carrying surface; 1321-through hole; 14-counterweight; 141-drainage hole; 15-identification member; 151-identification surface; 152-inserting part; 1521-positioning protrusion; 2-tube taking assembly; 21-outer shell; 211-embedded part; 2110-buffering space; 2111-half part; 212-clamping protrusion; 213-limiting groove; 22-push rod; 221-pressing part; 222-first stop part; 223-protruding part; 23-resetting member; 24-hollow limiting column; 241-hollow cylindrical segment; 2411-second stop part; 242-hollow circular table segment; 3-storage container; 31-box body; 311-placing position; 3111-limiting fitting part; 32-box cover; 321-flow-through opening. DETAILED DESCRIPTION

[0063] The technical solutions of the present disclosure will be described clearly and completely below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present disclosure.

[0064] In the description of the present disclosure, it should be noted that, if the terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like appear, the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present disclosure and simplifying the description, and therefore cannot be understood as indicating or implying that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present disclosure. In addition, if the terms "first", "second", "third" appear, they are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0065] In the description of the present disclosure, it should be noted that, unless otherwise explicitly specified and limited, if the terms "mounting", "connecting", "connection" appear, they should be understood in a broad sense, for example, can be fixedly connected, can be detachably connected, or integrally connected; can be mechanically connected, can be electrically connected; can be directly connected, or indirectly connected through an intermediate medium; can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present disclosure can be understood according to the specific circumstances.

[0066] Referring to Figures 1 to 20 As shown in the drawings, the embodiment provides a cryopreservation device, which comprises a cryopreservation tube assembly 1, a storage container 3 and a tube taking assembly 2, the cryopreservation tube assembly 1 comprises a tube cap 11, a tube body 12 and a carrier 13, the tube cap 11 is detachably connected with the tube body 12, the carrier 13 is connected with the tube cap 11, the carrier 13 has a carrier surface 132A for carrying a target sample, the carrier surface 132A is a curved surface, and the carrier surface 132A can extend into the tube body 12 to place the target sample in the tube body 12; the storage container 3 is provided with a placing position 311, and the tube body 12 can be limited in the placing position 311; the storage container 3 is used for being immersed in a freezing medium, and the freezing medium can flow into the tube body 12; the tube taking assembly 2 has a first station and a second station, at the first station, the tube taking assembly 2 can be connected with the tube cap 11 to separate the tube cap 11 from the tube body 12 or connect the tube cap 11 with the tube body 12, and at the second station, the tube taking assembly 2 can be separated from the tube cap 11.

[0067] The cryopreservation device provided by the embodiment can be used in the following manner: the tube body 12 is placed in the placing position 311 of the storage container 3, then the storage container 3 and the tube body 12 are immersed in a freezing medium, the freezing medium flows into the tube body 12; the tube taking assembly 2 is connected with the tube cap 11 at the first station, then the target sample is placed on the carrier surface 132A of the carrier 13 for freezing, and then the tube cap 11 is connected to the tube body 12 by the tube taking assembly 2, so that the target sample is immersed in the freezing medium, at this time, the tube taking assembly 2 is switched to the second station, so that the tube taking assembly 2 is separated from the tube cap 11, thereby completing the freezing operation. When the target sample needs to be taken out, the tube taking assembly 2 is connected with the tube cap 11 at the first station to separate the tube cap 11 from the tube body 12, so that the target sample is taken out of the tube body 12, and then the target sample is thawed. In the operation process, the operator does not need to directly contact the cryopreservation tube assembly 1 with hands, and the connection or separation of the tube cap 11 and the tube body 12 and the transfer of the cryopreservation tube assembly 1 are realized by the cooperation of the tube taking assembly 2 and the tube cap 11 and the cooperation of the storage container 3 and the tube body 12, thereby improving the work efficiency and the operation safety. Meanwhile, since the carrier surface is a curved surface, the target sample is placed on the curved surface, which can increase the contact area of the target sample and the carrier surface, so as to better overcome the influence of gravity, reduce the risk of sliding or loss of the target sample, and make the target sample more stable and reliable during storage and transfer.

[0068] Illustratively, the freezing medium can be liquid nitrogen. The target sample can be a biological sample such as a gamete, a zygote or an embryo.

[0069] In one embodiment, the tube body 12 has opposite first and second ends, the first end of the tube body is matched with the cap, the second end of the tube body is matched with the storage container, the diameter of the first end is larger than that of the second end, which not only facilitates the insertion of the carrier into the tube body 12 from the first end, but also facilitates the placement of the tube body in the placement position, and the smaller diameter of the second end can play a guiding role. At the same time, the tube body can load more frozen medium than the straw type sleeve, and can support the sample to be soaked in the frozen medium for a relatively long period of time during frequent sample access in daily life, which is safer for the sample.

[0070] In one embodiment, the inner wall of the tube body 12 is provided with a communication groove 121, which has a first end 1211 and a second end 1212 along the extension direction of the communication groove 121, the first end 1211 of the communication groove 121 penetrates the end face of the tube body 12 facing the cap 11, and the second end 1212 of the communication groove 121 is located in the tube body 12, and the communication groove 121 is configured to make the flow rate of the frozen medium from the first end 1211 to the second end 1212 greater than that from the second end 1212 to the first end 1211.

[0071] By providing the communication groove 121, the inflow and outflow rates of liquid nitrogen can be controlled, the inflow rate of liquid nitrogen into the tube body is increased, the target sample can be instantaneously frozen at ultra-low temperature during the freezing of the clinical operation, and at the same time, the outflow rate of liquid nitrogen from the tube body is slowed down, the liquid nitrogen in the tube body can be as slowly as possible to overflow and evaporate, thereby meeting the needs of the clinical operation.

[0072] In some embodiments, the width of the first end 1211 of the communication groove 121 is greater than that of the second end 1212 of the communication groove 121.

[0073] For example, as shown in Figure 3 As shown, the communication groove 121 can be a V-shaped groove, when the liquid nitrogen flows from the first end 1211 with a larger width to the second end 1212 with a smaller width, the flow rate can be increased, so that the liquid nitrogen quickly flows into the tube body to instantaneously freeze the target sample at ultra-low temperature. When the liquid nitrogen needs to overflow or evaporate from the tube body, it needs to flow from the second end 1212 with a smaller width to the first end 1211 with a larger width, and the speed is slowed down.

[0074] Of course, the communication groove 121 can also be a U-shaped groove.

[0075] In some embodiments, as shown in Figure 4 As shown, the communication groove 121 can also be a Tesla valve groove. When the liquid nitrogen flows from the first end 1211 to the second end 1212 of the Tesla groove, the flow rate increases, and when the liquid nitrogen flows from the second end 1212 to the first end 1211 of the Tesla groove, the flow rate decreases.

[0076] In one embodiment, the gap 10 is provided between the cap 11 and the tube 12 for the frozen medium to enter the tube 12. For example, the gap 10 is provided between the end faces of the cap 11 and the tube 12 close to each other, and the first end 1211 of the communication groove 121 penetrates the end face of the tube 12 facing the cap 11, so that the communication groove 121 communicates with the gap 10, liquid nitrogen can flow to the communication groove 121 through the gap, and enter the tube 12. At the same time, since the gap between the end faces of the cap 11 and the tube 12 close to each other in the embodiment is small, the small gap cooperates with the communication groove 121 to achieve the purpose of rapid entry of liquid nitrogen and slow down the overflow and volatilization speed of liquid nitrogen, and can also effectively filter and block fine impurities in the liquid nitrogen.

[0077] In one embodiment, the carrier includes a rod portion 131 and a plate portion 132, one end of the rod portion 131 is connected with the cap 11, the other end of the rod portion 131 is connected with the plate portion 132, and the carrier surface 132A is the plate surface of the plate portion.

[0078] For example, the rod portion 131 and the plate portion 132 are integrally formed, which is convenient for processing and can also reduce the risk of breakage and falling off.

[0079] It should be noted that the rod portion and the plate portion can also be two independent components, which are then fixedly connected together by insertion or adhesion.

[0080] For example, the cap 11 is provided with a plug-in pipe, the rod portion 131 is inserted into the plug-in pipe to achieve interference fit, and of course, the rod portion 131 can also be integrally formed with the cap 11.

[0081] For example, when the carrier includes a rod portion and a plate portion, the carrier can be an incomplete cylinder, which includes a first section and a second section, the first section can be the rod portion, the first section is connected with the cap, the second section is connected with the first section, the second section is provided with a carrier groove, and the groove bottom and at least part of the groove wall of the carrier groove form the carrier surface.

[0082] In some embodiments, the thickness of the plate portion is 0.05-0.25mm. The thickness of the plate portion can be the dimension of the plate portion in the depth direction of the through hole.

[0083] For example, the thickness of the plate portion can be, but is not limited to, 0.05mm, 0.06mm, 0.08mm, 0.1mm, 0.12mm, 0.14mm, 0.16mm, 0.18mm, 0.2mm, 0.22mm, 0.23mm, 0.24mm or 0.25mm.

[0084] Compared with the existing cylindrical structure, the carrier in the embodiment has a smaller volume, can reduce the occupation of the internal space of the tube, and thus enables more frozen medium to be loaded in the tube, which is more beneficial to the preservation of the target sample.

[0085] In some cases, the shape of the curved surface can be understood as the shape of the cross section of the plate portion.

[0086] In some embodiments, referring to Figure 7A As shown, the plate portion 132 can be an arc-shaped sheet, and the thickness thereof can be 0.05-0.25 mm. The shape of the cross section of the plate portion 132 is arc-shaped, that is, the carrier groove is an arc-shaped groove.

[0087] In some embodiments, the shape of the cross section of the plate portion 132 can also be V-shaped, that is, the carrier groove is a V-shaped groove.

[0088] In some embodiments, the shape of the cross section of the plate portion can also be wave-shaped.

[0089] In some embodiments, the shape of the cross section of the plate portion 132 can also be a concave shape. Specifically, the plate portion includes a transverse plate and two vertical plates, the two vertical plates are connected to the two side edges of the transverse plate respectively, and the two vertical plates are located on the same side of the transverse plate. The transverse plate can be an arc-shaped plate, and the formed carrier groove is a U-shaped groove. Of course, the transverse plate can also be a plate body with uneven thickness, for example, one plate surface of the transverse plate is a plane, and the other plate surface is a curved surface. Exemplarily, the curved surface can be an arc-shaped surface protruding toward the plane, and the arc-shaped surface can serve as a carrier surface at this time.

[0090] In one embodiment, referring to Figure 7B As shown, the carrier surface 132A is provided with a through hole 1321, which is used for positioning the target sample, and facilitates the frozen storage of the target sample and better guarantees the effect after the frozen storage and recovery.

[0091] Before freezing, the target sample needs to be dehydrated with a balancing liquid, and before being placed in liquid nitrogen, the target sample needs to be protected by a cryoprotective liquid. Since the target sample is attached with a small amount of cryoprotective liquid, it is equivalent to that the target sample is wrapped in a liquid drop, and this liquid drop is attached with surface tension. When the target sample is placed in the through hole, it can be attached to the position of the through hole. By providing the through hole, the freezing rate during vitrification freezing can be guaranteed to be faster.

[0092] In the embodiment, the through hole is provided on the relatively thin plate portion, and the carrier surface of the carrier is a curved surface. This not only guarantees that the target sample is not easy to fall off during the freezing of the target sample, but also improves the freezing rate. Moreover, during thawing, the thawing rate can also be improved, which is beneficial to maintaining the recovery rate and activity of the sample.

[0093] In one embodiment, the through hole is a circular hole, and the diameter of the circular hole is 0.2-0.7 mm.

[0094] For example, the diameter of the circular hole can be, but is not limited to, 0.2 mm, 0.22 mm, 0.24 mm, 0.26 mm, 0.28 mm, 0.3 mm, 0.35 mm, 0.4 mm, 0.45 mm, 0.5 mm, 0.55 mm, 0.6 mm, 0.65 mm, 0.66 mm, 0.68 mm, 0.69 mm, or 0.7 mm.

[0095] In this embodiment, the number of through holes is one, which ensures that the area of the through hole accounts for a small proportion on the plate part, which is beneficial to ensure the mechanical strength of the plate part. Each through hole corresponds to the placement of one target sample, which facilitates one-to-one traceability management of the target sample.

[0096] In one embodiment, the cap 11 is threadedly connected with the tube body 12, as shown in Figure 2 The tube body 12 is provided with a limiting portion 122, as shown in Figure 20 The storage container 3 is provided with a limiting matching portion 3111, which cooperates with the limiting matching portion 3111 to limit the rotation of the tube body 12 about its own axis.

[0097] For example, the cap 11 is provided with external threads, and the tube body 12 is provided with internal threads that cooperate with the external threads.

[0098] In this embodiment, the communication groove 121 penetrates the internal threads, that is, the internal threads are disconnected at the position where the communication groove 121 is provided.

[0099] When the tube cap 11 is connected or separated from the tube body 12 by using the tube taking assembly 2, the limiting portion 122 on the tube body 12 cooperates with the limiting matching portion 3111 on the storage container 3 to limit the rotation of the tube body 12 about its own axis, so that the cap 11 can rotate relative to the tube body 12, thereby achieving the connection or separation of the cap 11 and the tube body 12.

[0100] For example, the limiting portion 122 can be a first rotation limiting rib, and the limiting matching portion 3111 can be a second rotation limiting rib. The number of first rotation limiting ribs and second rotation limiting ribs can both be multiple. The multiple first rotation limiting ribs are arranged at intervals along the circumferential surface of the tube body 12, and the multiple second rotation limiting ribs are arranged on the placement site 311 and cooperate with the corresponding first rotation limiting ribs. When the cap 11 is screwed onto the tube body 12 by using the tube taking assembly 2, the second rotation limiting rib can abut against the first rotation limiting rib to limit the rotation of the tube body 12 about its own axis, thereby facilitating the screwing of the cap 11 onto the tube body 12.

[0101] For example, the profile shape of the first rotation limiting rib can be rectangular or triangular, such as an obtuse triangle.

[0102] It should be noted that the structural forms of the limiting part 122 and the limiting mating part 3111 are not limited to the one mentioned above. As long as they can achieve the function of limiting the rotation of the tube body 12 around its own axis so that the tube cap 11 can be smoothly connected or separated from the tube body 12, it is acceptable. For example, one of the limiting part 122 and the limiting mating part 3111 can be a slot, and the extension direction of the slot can be parallel to the axial direction of the tube body 12. The other can be a protrusion. During the process of placing the tube body 12 in the placement position 311, the protrusion can be inserted into the slot, and the groove wall of the slot abuts against the surface of the protrusion.

[0103] In other embodiments, the cap 11 and the tube body 12 can also be connected by a plug-in method. In this case, a first magnetic element can be provided on the placement position 311 of the storage container 3, and a second magnetic element can be provided on the end of the tube body 12 away from the cap 11. The first and second magnetic elements attract each other, thereby fixing the tube body 12. For example, the first and second magnetic elements can be two magnets that attract each other.

[0104] In one embodiment, see Figure 9 and Figure 10 As shown, the cryopreservation tube assembly 1 also includes a counterweight 14, which is installed at the end of the tube body 12 away from the tube cap 11, and the counterweight 14 is provided with a discharge hole 141.

[0105] In this embodiment, a partition is provided inside the tube to divide the internal space of the tube into a first space and a second space, with the first space being larger than the second space. The target sample is located in the first space, and the counterweight 14 is installed in the second space. Liquid nitrogen can flow through the gap 10 to the connecting groove 121 and enter the first space of the tube 12. Since the tube is immersed in liquid nitrogen, liquid nitrogen will also be present in the second space. The residual liquid nitrogen in the second space can be discharged through the discharge hole 141 of the counterweight 14, preventing the tube 12 from cracking due to energy accumulation during the vaporization of residual liquid nitrogen.

[0106] For example, the counterweight 14 can be a hollow cylinder. The counterweight 14 can be made of stainless steel, which has a density greater than that of liquid nitrogen, so that the cryopreservation tube assembly 1 can be immersed in liquid nitrogen, ensuring stable temperature inside the tube 12 and good cryopreservation effect.

[0107] It should be noted that in the scheme of setting a second magnetic element at the end of the tube body 12 away from the tube cap 11, the second magnetic element can be set on the side of the counterweight away from the tube cap 11.

[0108] In one embodiment, at least one of the counterweight and the tube is provided with a protrusion, which is used to restrict the axial movement of the counterweight 14 along the tube 12.

[0109] In some embodiments, the protrusions 123 are arranged on the inner wall of the end of the tube body 12 away from the tube cap, and the protrusions 123 abut against the surface of the weight block 14 away from the tube cap 11. See Figure 10 As shown, the protrusions 123 are integrally arranged on the tube body 12, and the number of the protrusions 123 can be multiple. The inner diameter of the end of the tube body 12 away from the tube cap 11 is slightly larger than the outer diameter of the weight block 14, and when the weight block 14 is loaded into the tube body 12, the protrusions 123 will abut against the surface of the weight block 14 away from the tube cap 11, achieving snap assembly and preventing the weight block 14 from falling off.

[0110] For example, the protrusions 123 can be spherical cap-shaped convex structures. Of course, the protrusions can also be strip-shaped convex ribs.

[0111] In other embodiments, the weight block 14 is interference-fitted with the tube body through protrusions (not shown in the figure).

[0112] For example, the protrusions can be arranged on the circumferential side wall of the weight block, or on the inner wall of the end of the tube body away from the tube cap, i.e., the cavity wall of the second space. Of course, the circumferential side wall of the weight block and the cavity wall of the second space of the tube body can both be provided with protrusions, and when assembled, the protrusions on the circumferential side wall of the weight block and the protrusions on the cavity wall of the second space can be staggered, enhancing the limiting effect.

[0113] It should be noted that the weight block 14 can also be fixedly installed on the tube body 12 through a buckle or the like.

[0114] In one embodiment, as shown in Figure 1 and Figure 8 The cryopreservation tube assembly 1 further includes an identification member 15, which is installed on the tube cap 11. The identification member 15 has an identification surface 151 facing away from the tube body 12, and the identification surface 151 is provided with sample information.

[0115] For example, the sample information can be a two-dimensional code, which can be laser-etched on the identification surface 151. The two-dimensional code can achieve automatic and fast storage and identification of sample information, and one-to-one traceability management of samples.

[0116] In some embodiments, as shown in Figure 7A , Figure 7B and Figure 8 The identification member 15 is provided with an insertion portion 152, which is provided with a positioning protrusion 1521, and the tube cap 11 is provided with a insertion hole, the insertion portion 152 can be inserted into the insertion hole, and the positioning protrusion 1521 can abut against the end surface of the tube cap 11 facing the tube body 12.

[0117] Exemplarily, the insertion part 152 can be integrally formed with the marker 15, and the insertion part 152 is located at a side of the marker 15 away from the marker surface 151. When the insertion part 152 is inserted into the insertion hole, the positioning protrusion 1521 abuts against an end surface of the cap 11 facing the tube body 12. At this time, the marker 15, the cap 11 and the carrier 13 are combined to form an integral whole, which can be connected with or separated from the tube body 12.

[0118] Referring to Figure 7A and Figure 7B , the insertion part 152 can indicate the orientation of the carrier surface 132A. In operation, the insertion part 152 is easily visible to an operator, and along the extension direction of the insertion part 152, the carrier surface can be determined, so that the target sample can be placed on the carrier surface (or in the through hole 1321 of the carrier surface), effectively preventing the operator from mistakenly placing the target sample on a side of the carrier away from the carrier surface.

[0119] In one embodiment, referring to Figures 11 to 14 , the tube taking assembly 2 includes a housing 21 and a push rod 22. The housing 21 is a hollow structure with both ends open. One of the housing 21 and the cap 11 is provided with an embedded part 211, and the other is provided with an embedded matching part. One of the embedded part 211 and the embedded matching part can be elastically deformed, so that the embedded part 211 is limited in the embedded matching part. The push rod 22 is slidably arranged in the housing 21. In a first working position, the embedded part 211 can be limited in the embedded matching part, so that the housing 21 and the cap 11 are fixedly connected. In a second working position, the push rod 22 can move relative to the housing 21, so that an end of the push rod 22 close to the embedded part 211 abuts against the cap 11, and the embedded part 211 is separated from the embedded matching part.

[0120] Referring to Figure 11 , an end of the push rod 22 away from the embedded part 211 is provided with a pressing part 221. The pressing part 221 is located outside the housing 21, and the cross-sectional area of the pressing part 221 is greater than that of the housing 21, so that the pressing part 221 cannot enter the housing 21. In use, the pressing part 221 can be operated to drive the push rod 22 to slide relative to the housing 21.

[0121] In some embodiments, referring to Figure 6 and Figure 12As shown, the housing 21 is provided with an embedding portion 211 located at one end of the housing 21, and the cap 11 is provided with an embedding matching portion which can be a receiving groove 111. When the embedding portion 211 is inserted into the receiving groove 111, the embedding portion 211 can be elastically deformed so that the embedding portion 211 can be inserted into the receiving groove 111, and then when the embedding portion 211 is fully or partially restored to its original shape, the embedding portion 211 abuts against the groove wall of the receiving groove 111 so that the embedding portion 211 is limited in the receiving groove 111. In the first station, the connection of the embedding portion 211 and the embedding matching portion enables the fixed connection of the housing 21 of the pipette assembly 2 and the cap 11, thereby realizing the connection or separation of the cap 11 and the tube body 12 and also realizing the transfer of the cryo tube assembly 1.

[0122] Since the push rod 22 is slidably arranged in the housing 21, in the second station, the push rod 22 is moved relative to the housing 21 so that the end of the push rod 22 close to the embedding portion 211 protrudes outside the housing 21 and abuts against the cap 11, that is, the end of the push rod 22 protruding outside the housing 21 can push against the groove bottom of the receiving groove 111 so that the embedding portion 211 is separated from the receiving groove 111, thereby realizing the separation of the pipette assembly 2 and the cryo tube assembly 1.

[0123] After the separation of the pipette assembly 2 and the cryo tube assembly 1, the push rod 22 can be moved so that the end of the push rod 22 close to the embedding portion 211 is retracted into the housing 21, so as to facilitate the connection of the pipette assembly 2 and the cap 11 of the next cryo tube assembly 1.

[0124] In an embodiment, the cross-sectional profile of the groove wall of the receiving groove 111 is a concave polygon.

[0125] For example, as shown in Figure 6 As shown, the cross-sectional profile of the groove wall of the receiving groove 111 is an octastar shape.

[0126] Correspondingly, the outer cross-sectional profile of the embedding portion 211 is generally matched with the cross-sectional profile of the groove wall of the receiving groove 111. For example, as shown in Figure 12 As shown, the embedding portion 211 includes two halves 2111 which have a buffer space 2110 therebetween so that the two halves 2111 can be elastically deformed towards each other, and each of the two halves 2111 has four corners to respectively match the eight corners of the receiving groove 111.

[0127] Meanwhile, as shown in Figure 8 As shown, the cross-sectional shape of the marker 15 is also an octastar shape so as to be inserted into the receiving groove 111.

[0128] The embedding portion is not limited to the above structure. For example, the embedding portion can include a plurality of sub-portions arranged along the circumference of the housing. For example, the number of sub-portions can be four, and each sub-portion has two corners. In this case, the buffer space can be a cross-shaped slot.

[0129] Of course, the cross section of the slot wall of the accommodation slot 111 can also be a hexagonal star shape. Correspondingly, each of the two halves 2111 has three corners. For the case where the embedding portion includes a plurality of sub-portions, the number of sub-portions can be three, and each sub-portion has two corners.

[0130] It should be noted that the cross section of the slot wall of the accommodation slot 111 is not limited to a concave polygon, but can also be a convex polygon, an ellipse, etc., as long as the embedding portion 211 cannot rotate relative to the accommodation slot 111.

[0131] In one embodiment, the embedding portion 211 is provided with a clamping portion, and the embedding fitting portion is provided with a clamping fitting portion. When the embedding portion 211 returns to its original state, the clamping fitting portion can cooperate with the clamping portion to fix the connection between the housing 21 and the cap 11, which can further enhance the connection stability between the tube taking assembly 2 and the cryopreservation tube assembly 1.

[0132] For example, the clamping portion is arranged on the outer surface of the side wall of the embedding portion 211, and the clamping fitting portion is arranged on the slot wall of the accommodation slot 111.

[0133] In some embodiments, referring to Figure 1 , Figure 6 and Figure 12 , the clamping portion is a clamping protrusion 212, and the clamping fitting portion is a clamping groove 112. In the first station, the clamping protrusion 212 is limited in the clamping groove 112, and in the second station, the clamping protrusion 212 is separated from the clamping groove 112.

[0134] In other embodiments, the clamping portion is a clamping groove, and the clamping fitting portion is a clamping protrusion.

[0135] For example, the surfaces of the clamping protrusion 212 and the clamping groove 112 that contact each other are spherical cap curved surfaces.

[0136] Referring to Figure 1 , Figure 6 and Figure 12 , the clamping protrusion 212 can be a hemispherical protrusion, and the clamping groove 112 can be a hemispherical groove. When the hemispherical protrusion is limited in the hemispherical groove, the embedding portion 211 can return to its original state.

[0137] Of course, the clamping protrusion 212 can also be a protrusion with a volume greater than a hemisphere, or a protrusion with a volume less than a hemisphere.

[0138] Exemplarily, the surface of the clamping protrusion 212 and the surface of the clamping groove 112 in mutual contact can also be ellipsoidal surfaces.

[0139] In some embodiments, each half is provided with three clamping protrusions 212.

[0140] In some embodiments, when the number of sub-parts is four, each sub-part is provided with one clamping protrusion 212.

[0141] In one embodiment, as shown in Figure 14 and Figure 16 The tube taking assembly 2 further comprises a reset member 23; the push rod 22 is provided with a first stop portion 222, the shell 21 is provided with a second stop portion 2411, the reset member 23 is located between the first stop portion 222 and the second stop portion 2411, and the reset member 23 can enable the push rod 22 to switch from the second station to the first station. In this way, the quick switching of the two stations can be realized.

[0142] Exemplarily, the reset member 23 can be a compression spring. When the pressing portion 221 is pressed, the distance between the first stop portion 222 and the second stop portion 2411 is reduced, the compression spring is compressed, and the end of the push rod 22 protrudes from the embedded portion 211 to abut against the groove bottom of the accommodating groove 111, so as to separate the tube taking assembly 2 from the cryopreservation tube assembly 1. After the tube taking assembly 2 is separated from the cryopreservation tube assembly 1, the pressing portion 221 is released, the compression spring returns to its original state, and the push rod 22 is reset.

[0143] In some embodiments, as shown in Figure 14 and Figure 16 The tube taking assembly 2 further comprises a hollow limiting column 24, which is installed in the shell 21, and the push rod 22 is arranged in the hollow limiting column 24, and the second stop portion 2411 is an end face of the hollow limiting column 24 facing the reset member 23.

[0144] Exemplarily, the hollow limiting column 24 can be fixedly installed in the shell 21. The shell 21 comprises a first tube segment, a second tube segment and a third tube segment connected in sequence. The first tube segment can be a constant diameter segment, the second tube segment can be a tapered segment, and the diameter of the tapered segment gradually decreases from the first tube segment to the third tube segment. The inner diameter of the third tube segment is substantially equal to the smaller inner diameter of the tapered segment. The inner diameter of the third tube segment is slightly larger than the diameter of the push rod 22, so that the push rod 22 can reciprocate relative to the shell 21.

[0145] As shown in Figure 14 The outer surface of the hollow limiting column 24 is matched with the inner surfaces of the first tube segment and the second tube segment, and the inner diameter of the hollow limiting column 24 is substantially equal to the inner diameter of the third tube segment. As shown in Figure 16As shown, the hollow limiting column 24 includes a hollow cylindrical segment 241 and a hollow circular truncated cone segment 242, the hollow cylindrical segment 241 is located in the first tube segment, and the hollow circular truncated cone segment 242 is located in the second tube segment. The second stop 2411 is an end face of the free end of the hollow cylindrical segment 241.

[0146] In one embodiment, referring to Figure 15 As shown, the shell 21 is provided with a limiting groove 213, the extending direction of the limiting groove 213 is consistent with the length direction of the shell 21; the push rod 22 is provided with a protruding part 223, the protruding part 223 can reciprocate along the extending direction of the limiting groove 213, and in the first station, the protruding part 223 can abut against one end of the limiting groove 213 away from the tube cap 11.

[0147] When the pressing part 221 is pressed, it can be pressed in place at one time, until the protruding part 223 abuts against one end of the limiting groove 213 close to the tube cap 11, the compression spring is compressed, and the end of the push rod 22 protrudes from the embedding part 211 to abut against the groove bottom of the accommodating groove 111, so as to separate the tube taking assembly 2 from the cryogenic tube assembly 1. When the pressing part 221 is released, the compression spring returns to its original state, so that the push rod 22 is reset, and the tube taking assembly 2 is switched from the second station to the first station, at this time, the protruding part 223 abuts against one end of the limiting groove 213 away from the tube cap 11, so as to limit the movement stroke of the push rod 22.

[0148] In other embodiments, an embedding fitting part can also be provided on the shell 21, and an embedding part can be provided on the tube cap 11. Illustratively, the embedding fitting part can include two clamping arms with a gap therebetween and capable of elastic deformation; the embedding part can be integrally formed with the tube cap 11, or the embedding part can be part of the tube cap 11, and in use, the tube taking assembly 2 can clamp the tube cap 11.

[0149] In one embodiment, referring to Figures 17 to 20 As shown, the storage container 3 includes a box body 31 and a box cover 32, the box cover 32 is detachably covered on the box body 31; a placing site 311 is provided on the box body 31, the number of the placing sites 311 is multiple, the multiple placing sites 311 are arranged in rows and columns in the box body 31, and the box body 31 is provided with a mark to determine the position information of the cryogenic tube assembly in different placing sites.

[0150] The number of the placing sites 311 is set to m, referring to Figure 18 and Figure 20As shown, each placement site 311 is designed with a plurality of limiting cooperation parts 3111, such as a second rotation limiting rib, to cooperate with the first rotation limiting rib on the pipe body 12, thereby limiting the rotation of the pipe body 12 along its own axis. The bottom plate of the box body 31 can be square, and the transverse and longitudinal directions of the bottom plate are provided with marks, having the functions of position marking and management. For example, the transverse marks of the bottom plate include X1, X2, …, Xn, and the longitudinal marks include Y1, Y2, …, Yn, where m=n 2 The m placement sites 311 are respectively located at the orthogonal positions of each transverse and longitudinal direction, which can realize the marking of matrix position information. For example, the position information of the cryopreservation tube assembly 1 located at the four corner positions can be marked as X1Y1, XnY1, X1Yn and XnYn, respectively.

[0151] For example, referring to Figure 20 As shown, the number of placement sites 311 is 64, that is, in this embodiment, m=64 and n=8.

[0152] It should be noted that the marking is not limited to the above form. For example, taking 64 placement sites as an example, the marking can be provided on two adjacent side walls of the box body, wherein the marking on one side wall can include letters, such as A, B, C, D, E, F, G and H, and the marking on the other side wall can include numbers, such as 1, 2, 3, 4, 5, 6, 7 and 8. At this time, the position information of the cryopreservation tube assembly 1 located at the four corner positions can be marked as A1, H1, A8 and H8, respectively.

[0153] The marking can be provided on the side wall of the box body in various ways, for example, the marking can be integrally injection molded with the side wall, or can be attached to the side wall.

[0154] In some embodiments, the position information represented by the marking can also be saved in a database, and the cryopreservation device can further include a controller. The controller can extract the position information recorded by the marking from the database, and generate a control instruction according to the position information to control the robot and the like. The control instruction can include a placement instruction and a taking-out instruction. The placement instruction is used to instruct the robot to load the carrier in the pipe body corresponding to the position information, and the taking-out instruction is used to instruct the robot to take out the cryopreservation tube assembly from the pipe body corresponding to the position information. The controller is used to control the robot and the like to load the carrier with the sample into the pipe body at a specific position, or to control the robot and the like to take out the cryopreservation tube assembly or the carrier at a specific position by using the tube taking-out assembly.

[0155] Since the identification member has an identification surface provided with sample information, the sample information is one-to-one corresponding to the position information recorded by the marking. Therefore, not only the automatic and fast storage and identification of the sample information can be realized, but also the one-to-one traceability management of the sample can be realized.

[0156] In some embodiments, referring to Figure 18 As shown, the box cover 32 is provided with a flow-through opening 321.

[0157] Exemplarily, the opposite two sides of the box cover 32 are both provided with the flow-through opening 321. The flow-through opening 321 can be, but is not limited to, a rectangular hole. Liquid nitrogen can enter the inside of the box body 31 from the flow-through opening 321 and the gap between the box cover 32 and the box body 31.

[0158] It should be noted that the shape and material of the storage container 3 are not limited, as long as the use requirements can be met.

[0159] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present disclosure, and not to limit them; although the present disclosure has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present disclosure.

Claims

1. A cryogenic storage device, characterized by, The application relates to a cryopreservation tube assembly, a storage container and a tube taking assembly. The application relates to a cryopreservation tube assembly, a storage container and a tube taking assembly. The inner wall of the tube body is provided with a communication groove, the communication groove has a first end and a second end along the extension direction of the communication groove, the first end of the communication groove penetrates through the end surface of the tube body facing the tube cap, and the second end of the communication groove is located in the tube body. The first end of the communication groove has a width greater than that of the second end of the communication groove, or the communication groove is a Tesla valve groove.

2. The cryoablation device of claim 1, wherein, The curved surface has an arc shape, a V shape, a wave shape, a trapezoidal shape or a concave shape.

3. The cryo device of claim 2, wherein, The carrying surface is provided with a through hole for positioning the target sample.

4. The cryoablation device of claim 1, wherein, The through hole is a circular hole with a diameter of 0.2-0.7 mm.

5. The cryoablation device of claim 1, wherein, The tube cap is threadedly connected with the tube body, the tube body is provided with a limiting portion, the storage container is provided with a limiting matching portion, and the limiting portion and the limiting matching portion are matched to limit the rotation of the tube body around its own axis.

6. The cryoablation device of claim 5, wherein, The tube taking assembly comprises a shell and a push rod, the shell is a hollow structure with open ends, one of the shell and the tube cap is provided with an embedding portion, and the other is provided with an embedding matching portion, at least one of the embedding portion and the embedding matching portion can be elastically deformed to limit the embedding portion in the embedding matching portion.

7. The cryoablation device of claim 1, wherein, The push rod is slidably arranged in the shell, in the first position, the embedding portion can be limited in the embedding matching portion to fixedly connect the shell with the tube cap, and in the second position, the push rod can be moved relative to the shell to make the end of the push rod close to the embedding portion abut against the tube cap and make the embedding portion separate from the embedding matching portion.

8. The cryoablation device of claim 1, wherein, The embedding portion is provided with a clamping portion, the embedding matching portion is provided with a clamping matching portion, and the clamping matching portion can be matched with the clamping portion to fixedly connect the shell with the tube cap. ​ 9. The cryoablation device of claim 8, wherein, ​ 10. The cryogenic storage device of claim 9, wherein, The embedding part is arranged at one end of the shell, and can be elastically deformed. The embedding fitting part is arranged at an end of the cap away from the tube body, and is a receiving groove. The clamping fitting part is arranged on the groove wall of the receiving groove. One of the clamping part and the clamping fitting part is a clamping protrusion, and the other is a clamping groove. In the first working position, the clamping protrusion is limited in the clamping groove. In the second working position, the clamping protrusion is separated from the clamping groove.

11. The cryogenic storage device of claim 10, wherein, The cross-sectional profile of the groove wall of the receiving groove is a concave polygon; and / or, the surfaces of the clamping protrusion and the clamping groove in contact with each other are spherical cap curved surfaces or ellipsoidal surfaces.

12. The cryoablation device of claim 8, wherein, The tube taking assembly further comprises a reset member. The push rod is provided with a first stop part, and the shell is provided with a second stop part. The reset member is located between the first stop part and the second stop part. The reset member can enable the push rod to switch from the second working position to the first working position.

13. The cryogenic storage device of claim 12, wherein, The tube taking assembly further comprises a hollow limiting column. The hollow limiting column is mounted in the shell. The push rod passes through the hollow limiting column. The second stop part is an end face of the hollow limiting column facing the reset member.

14. The cryogenic storage device of claim 8, wherein, The shell is provided with a limiting groove. The extending direction of the limiting groove is consistent with the length direction of the shell. The push rod is provided with a protruding part. The protruding part can reciprocally move along the extending direction of the limiting groove. In the first working position, the protruding part can abut against an end of the limiting groove away from the cap.

15. The cryo device according to any one of claims 1 to 14, characterized in that The cryopreservation tube assembly further comprises an identification member. The identification member is mounted on the cap. The identification member has an identification surface. The identification surface faces a side of the cap away from the tube body. The identification surface is provided with sample information.

16. The cryogenic storage device of claim 15, wherein, The identification member is provided with an insertion part. The insertion part is provided with a positioning protrusion. The cap is provided with a insertion hole. The insertion part can be inserted into the insertion hole. The positioning protrusion can abut against an end face of the cap facing the tube body.

17. The cryo device of any one of claims 1 to 14, wherein, The cryopreservation tube assembly further comprises a counterweight. The counterweight is mounted at an end of the tube body away from the cap. The counterweight is provided with a discharge hole.

18. The cryogenic storage device of claim 17, wherein, At least one of the counterweight and the tube body is provided with a protruding part. The protruding part is used to limit the axial movement of the counterweight along the tube body.

19. The cryogenic storage device of claim 18, wherein, The protruding part is arranged on an inner wall of an end of the tube body away from the cap. The protruding part abuts against a surface of the counterweight away from the cap.

20. The cryogenic storage device of claim 18, wherein, The counterweight is in interference fit with the tube body through the protruding part. The protruding part is arranged on a circumferential side wall of the counterweight, and / or the protruding part is arranged on an inner wall of an end of the tube body away from the cap.

21. The cryogenic storage device of any one of claims 1 to 14, wherein, There is a gap between the cap and the tube body for the frozen medium to enter the tube body.

22. The cryogenic storage device of any one of claims 1 to 14, wherein, The storage container comprises a box body and a box cover. The box cover is detachably arranged on the box body. The placing positions are arranged on the box body. The number of the placing positions is multiple. The multiple placing positions are arranged in rows and columns in the box body. The box body is provided with marks to determine the position information of the cryopreservation tube assemblies in different placing positions.

23. The cryogenic storage device of claim 22, wherein, The box cover is provided with a flow-through opening.

24. The cryogenic storage device of any one of claims 1 to 14, wherein, The carrier comprises a rod part and a plate part, one end of the rod part is connected with the pipe cap, the other end of the rod part is connected with the plate part, and the bearing surface is the plate surface of the plate part.

25. The cryogenic storage device of claim 24, wherein, The thickness of the plate part is 0.05-0.25 mm.

Images