Blue copper peptide microemulsion as well as preparation method and application thereof

Abstract

According to the blue copper peptide microemulsion and the preparation method and application thereof, the blue copper peptide microemulsion is prepared by adopting a W / O / W double emulsion system, blue copper peptide is wrapped in an internal water phase (W1), an outer layer forms double barriers and double protection through an oil phase (O) and an external water phase (W2), and the stability is greatly improved. Meanwhile, the internal water phase can efficiently load the water-soluble blue copper peptide, the oil phase layer adopts a formula of caprylic / capric triglyceride and butanediol, so that the fusion of the emulsion and the skin cuticle (lipid structure) can be promoted, and the hyaluronic acid of the external water phase improves the transdermal absorption and ensures that the skin feeling is fresh and cool. The double emulsification structure can delay the release speed of the blue copper peptide on the skin surface layer and realize the slow release effect, so that the active ingredients can permeate into the corium layer for a long time and act on fibroblasts, and the transdermal efficiency is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a blue copper peptide microemulsion, its preparation method and application. BACKGROUND

[0002] Blue copper peptide, English name GHK-Cu, is a tripeptide chain composed of glycine, histidine and lysine complexed with a divalent copper ion (Cu²⁺). In the field of beauty, blue copper peptide is a popular skin care ingredient, widely used in skin care products such as cream, serum, and mask, for anti-aging, repair, and moisturizing, etc. Blue copper peptide is an early signal molecule for tissue repair, which can promote the proliferation of epidermal stem cells and accelerate wound healing. At the same time, it can stimulate fibroblasts to synthesize glycosaminoglycans, collagen and elastin, etc., remodel the skin structure, repair the damaged skin barrier, and clinical data shows that its improvement rate on postoperative erythema of medical aesthetics can reach 43%. It can enhance the function of fibroblasts, promote the synthesis of collagen and elastic fibers, reduce fine lines and wrinkles, and experiments have proved that continuous use for 8 weeks can improve skin tightness by 17%-18%. In addition, blue copper peptide can regulate the activity of matrix metalloproteinases and their tissue inhibitors, maintain the balance between matrix degradation and synthesis, and improve the appearance of skin aging. It can also enhance the activity of superoxide dismutase and inhibit the generation of reactive oxygen free radicals, thereby playing an antioxidant role. At the same time, it can reduce the levels of acute inflammatory cytokines such as transforming growth factor-β and tumor necrosis factor-α, and inhibit inflammatory reactions.

[0003] Blue copper peptide is sensitive to light, heat and oxygen, and is prone to decomposition and failure. It may also cause copper ions to separate due to changes in pH, affecting its activity. It has a blue color, and high-concentration addition may cause skin care products to discolor, affecting product appearance. Precipitation or off-flavor may occur in some formulations, reducing user experience. The present application uses microemulsion encapsulation technology to isolate the external environment, protect its structure, and promote transdermal absorption. SUMMARY

[0004] The present application aims to provide a blue copper peptide microemulsion.

[0005] Still another object of the present application is to provide a preparation method for the above-mentioned blue copper peptide microemulsion.

[0006] Still another object of the present application is to provide an application of the above-mentioned product.

[0007] The object of the present application is achieved by the following scheme: a blue copper peptide microemulsion is prepared from the following ingredients in the following mass percentages: blue copper peptide 0.04 - 0.4 wt%, sodium hyaluronate 0.3 - 3 wt%, safflower acid glyceride 1 - 10 wt%, Butanediol 3 - 9 wt%, Xanthan gum 0.05 - 2 wt % Purified water balance.

[0008] The present application provides a preparation method of blue copper peptide microemulsion, which is prepared by using a W / O / W double emulsion system. The blue copper peptide is wrapped in the internal water phase (W1), and the outer layer forms a double barrier through the oil phase (O) and the external water phase (W2). The preparation method comprises the following steps: (1) Dissolve caprylic capric triglyceride in butanediol, and stir uniformly to obtain system A; (2) Dissolve blue copper peptide in a small amount of aqueous solution, add the blue copper peptide aqueous solution to system A, and disperse for several minutes to form a stable primary emulsion B; (3) Dissolve xanthan gum and sodium hyaluronate in 50-60 ℃ water in sequence, until colorless and transparent, and then cool to room temperature to obtain system C; add 5-20 parts by volume to system B, and disperse for several minutes to obtain blue copper peptide microemulsion.

[0009] The blue copper peptide microemulsion is prepared by using a W / O / W double emulsion system.

[0010] The molecular weight of sodium hyaluronate is 30kDa-900kDa, and the mass ratio of blue copper peptide to sodium hyaluronate is 1:1-1:9. In steps (1) and (2), the rotation speed of high-speed dispersion is 500-35000 rpm, and the treatment time is preferably 20 min.

[0011] The particle size distribution of the blue copper peptide microemulsion is 50-300 nm.

[0012] The present application provides an application of the blue copper peptide microemulsion in the preparation of cosmetics.

[0013] The present application aims to provide a preparation method of blue copper peptide microemulsion, which can improve the stability of blue copper peptide, promote its transdermal absorption, and reduce irritation.

[0014] As an early signal molecule for tissue repair, blue copper peptide can directly stimulate fibroblast proliferation, accelerate wound healing and epidermal regeneration, and promote ceramide synthesis, which has a clear soothing effect on sensitive skin, acne skin and barrier-damaged skin. It can activate fibroblasts to synthesize type I, type III collagen and proteoglycans, and has a better ability to stimulate collagen precursor synthesis than traditional ingredients such as retinoic acid and vitamin C. In combination with the antioxidant properties, it can neutralize free radicals and delay photoaging, and improve uneven skin tone by regulating melanin transport. The present application solves the key problems of poor stability and insufficient transdermal absorption efficiency of blue copper peptide in cosmetic applications.

[0015] The beneficial effects brought by the technical scheme of the present application The present application has the following outstanding advantages: the present application adopts a W / O / W double emulsion system to prepare a blue copper peptide microemulsion, the blue copper peptide is wrapped in the internal water phase (W1), the outer layer forms a W / O / W double barrier through the oil phase (O) and the external water phase (W2), which isolates light, heat, oxygen and the interference of conflicting components (such as strong acid, VC) in the formula. Avoid direct contact of blue copper peptide with the external environment, reduce the separation of copper ions and structural decomposition, the activity retention rate is increased by more than 30% compared with traditional O / W emulsion, double protection, the stability is greatly improved. At the same time, the internal water phase can efficiently load water-soluble blue copper peptide, the oil phase layer can promote the fusion of the emulsion and the stratum corneum (lipid structure), and the external water phase can ensure the skin feel. The double emulsification structure can delay the release speed of blue copper peptide on the skin surface, realize the "sustained release effect", make the active ingredients penetrate into the dermis more persistently, act on fibroblasts, and improve the transdermal efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is a transmission electron micrograph of the blue copper peptide microemulsion of the present application. DETAILED DESCRIPTION

[0017] The preparation method of the blue copper peptide emulsion microsphere of the present application will be further described below in combination with examples.

[0018] A blue copper peptide microemulsion is prepared by adopting a W / O / W double emulsion system, the blue copper peptide is wrapped in the internal water phase (W1), the outer layer forms a double barrier through the oil phase (O) and the external water phase (W2), which is prepared from the following components with mass percentage: 0.04 -0.4 wt% blue copper peptide, 0.3 -3 wt% sodium hyaluronate, 1 -10 wt% capric acid triglyceride, 3 -9 wt% butanediol, 0.05 -2 wt% xanthan gum The balance is purified water; wherein, The blue copper peptide microemulsion is prepared according to the following steps: (1) 1 -10 wt% capric acid triglyceride is dissolved in 3 -9 wt% butanediol, and stirred uniformly to obtain system A; (2) 0.04 -0.4 wt% blue copper peptide is dissolved in a small amount of water, and the blue copper peptide aqueous solution is added to system A, and dispersed for several minutes to form a stable primary emulsion B; (3) 0.05 -2 wt% xanthan gum and 0.3 -3 wt% sodium hyaluronate are sequentially dissolved in 50-60 ℃ water under stirring until colorless and transparent, and then cooled to room temperature to obtain system C. 5~20 parts by volume of system C is added to system B, and dispersed for several minutes to obtain a blue copper peptide microemulsion.

[0019] Table 1. Main components of blue copper peptide microemulsion: The remainder in each embodiment is purified water.

[0020] Transmission electron microscopy image of W / O / W blue copper peptide microemulsion is shown below. Figure 1 As shown, the retention rate of copper peptide activity is more than 30% higher than that of traditional O / W emulsions, providing dual protection and significantly improving stability. Simultaneously, the internal aqueous phase efficiently loads water-soluble copper peptide, the oil phase promotes the integration of the emulsion with the skin's stratum corneum (lipid structure), and the external aqueous phase ensures a refreshing feel. This dual emulsification structure slows down the release rate of copper peptide on the skin surface, achieving a "slow-release effect," allowing the active ingredients to penetrate deeper into the dermis and act on fibroblasts, thus improving transdermal efficiency.

Claims

1. A blue copper peptide microemulsion characterized in that, Formulated from the following ingredients in mass percentage: Blue copper peptide 0.04 - 0.4 wt%, Sodium hyaluronate 0.3 - 3 wt%, Glyceryl caprylocaprate 1 - 10 wt%, Butylene glycol 3 - 9 wt%, Xanthan gum 0.05 - 2 wt% Purified water balance.

2. A process for the preparation of the blue copper peptide microemulsion according to claim 1, characterized in that, The blue copper peptide microemulsion is prepared by using a W / O / W double emulsion system, the blue copper peptide is wrapped in the inner water phase (W1), and the outer layer forms a double barrier through the oil phase (O) and the outer water phase (W2), including the following steps: (1) Dissolve glyceryl caprylocaprate in butylene glycol, stir evenly, and obtain system A; (2) Dissolve blue copper peptide in a small amount of water solution, add the blue copper peptide aqueous solution to system A, and disperse for several minutes to form a stable primary emulsion B; (3) Dissolve xanthan gum and sodium hyaluronate in 50-60 ℃ water in turn, stir until colorless and transparent, and cool to room temperature to obtain system C; add 5-20 parts by volume to system B, and disperse for several minutes to obtain a blue copper peptide microemulsion.

3. The method for preparing a blue copper peptide microemulsion according to claim 2, characterized in that... The blue copper peptide microemulsion is prepared by using a W / O / W double emulsion system.

4. The method for preparing a blue copper peptide microemulsion according to claim 2, characterized in that... The molecular weight of sodium hyaluronate is 30kDa - 900kDa, and the mass ratio of blue copper peptide to sodium hyaluronate is 1:1 - 1:

9. In steps (1) and (2), the high-speed dispersion rotation speed is 500-35000 rpm, and the processing time is preferably 20 min.

5. The blue copper peptide microemulsion according to claim 1, characterized in that The particle size distribution of the blue copper peptide microemulsion is 50-300 nm.

6. Use of the blue copper peptide microemulsion according to claim 1 in the preparation of cosmetics.

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