Baicao gynecological inflammation clear water gel and preparation method thereof

Abstract

The invention relates to the technical field of medical treatment, in particular to herbal gynecological inflammation clear water gel, which is prepared from the following components in percentage by mass: 14 percent of traditional Chinese medicine extracting solution, 47.5 percent of matrix and 38.5 percent of matrix, the matrix 1 is N, O-carboxymethylated chitosan, and the matrix 2 is L-leucine. The hydrogel preparation adopted by the invention can be tightly attached to the surface of a vaginal mucosa by virtue of a specific three-dimensional network structure and good elasticity, so that more uniform coating and more lasting retention are realized, the loss of medicinal components caused by the self-cleaning effect of the vagina is reduced, and the effect that the medicine can continuously act on an inflammation part is ensured; the hydrogel prepared by the method is soft in texture, high in biocompatibility, almost free of irritation and foreign body sensation after being used and smoother and milder in administration process, and the comfortable use experience is beneficial to relieving psychological burdens of patients and enhancing trust and cooperation of the patients with treatment schemes.

Description

Technical Field

[0001] This invention relates to the field of medical technology, and more specifically, to a herbal gynecological anti-inflammatory gel and its preparation method. Background Technology

[0002] Vaginitis is one of the most common gynecological diseases, affecting the vaginal mucosa and submucosal connective tissue. The causes of vaginitis are complex, involving multiple factors such as bacterial, trichomonal, and viral infections, making it prone to recurrence and difficult to cure, causing significant physical and psychological harm to patients.

[0003] Baicao Fuyanqing Suppositories are a marketed traditional Chinese medicine suppository used for fungal, bacterial, and trichomonal vaginitis and cervical erosion. Its ingredients include Sophora flavescens, Stemona japonica, Cnidium monnieri, Callicarpa macrophylla, and Agrimonia pilosa, and it is a brownish-yellow to brownish-brown suppository. Clinical use has revealed that this suppository has poor adhesion and struggles to completely adhere to the irregular vaginal walls. Especially after it begins to dissolve in the body, the suppository itself starts to detach from direct contact with the vaginal wall, affecting the delivery and absorption of the active ingredients. Furthermore, due to the vagina's self-cleaning function, the active ingredients are often lost.

[0004] Hydrogels are a class of materials with a three-dimensional network structure, formed by covalent or non-covalent bonds of natural or synthetic polymers. They typically possess excellent elasticity and good biocompatibility, and do not cause toxic side effects or immune rejection. In gynecological applications, hydrogel formulations, due to their unique adhesiveness, spreadability, and biocompatibility, can better contact and remain on the vaginal wall, promoting the absorption of active pharmaceutical ingredients and ensuring therapeutic efficacy. This invention, while retaining most of the active ingredients of Baicao Fuyanqing suppositories, specifically develops and manufactures a hydrogel formulation, providing an effective, convenient, safe, and comfortable new product for the treatment of female vaginal inflammation. Summary of the Invention

[0005] To overcome the above deficiencies, the present invention provides a solution that overcomes or at least partially solves the above technical problems.

[0006] This invention provides a herbal gynecological anti-inflammatory hydrogel, which is composed of the following components by mass percentage: herbal extract: 14%, matrix: 47.5%, matrix: 38.5%, wherein matrix 1 is N,O-carboxymethylated chitosan, and matrix 2 is L-leucine.

[0007] Preferably, the herbal extract is prepared by water extraction, alcohol precipitation, and concentration of Chinese medicinal materials including Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla.

[0008] Preferably, the medicinal materials and their weight parts for preparing the herbal extract are: 600-700 parts of Sophora flavescens, 300-350 parts of Stemona japonica, 300-350 parts of Cnidium monnieri, 300-350 parts of Agrimonia pilosa, and 300-350 parts of Callicarpa macrophylla.

[0009] Preferably, a method for preparing a herbal gynecological anti-inflammatory hydrogel includes the following steps:

[0010] a) Preparation of Chinese herbal extract: Take Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla, add water and decoct, combine the decoctions, filter, and concentrate to obtain a clear extract; add ethanol to the clear extract to make the alcohol content reach the predetermined concentration, let stand, take the supernatant, filter, recover the ethanol and concentrate to obtain a thick extract, which is used as the Chinese herbal extract for later use.

[0011] b) Preparation of matrix 1 solution: N,O-carboxymethylated chitosan was added to purified water and stirred. Glacial acetic acid was added and stirring was continued until completely dissolved to obtain solvent ①.

[0012] c) Preparation of matrix 2 solution: L-leucine was added to purified water and stirred, then heated until completely dissolved to obtain solvent ②;

[0013] d) Mixing and preparing gel: Slowly add the Chinese herbal extract obtained in step a) to the solvent ① obtained in step b) while stirring. After mixing evenly, add the solvent ② obtained in step c) and stir until evenly mixed. Adjust the pH value to the predetermined range to obtain the gel solution.

[0014] e) Filling and sterilization: Fill the gel solution obtained in step d) into a medical container and sterilize it to obtain the final product.

[0015] Preferably, in step a), the decoction is performed three times, with the first and second decoctions each lasting 2 hours, and the third decoction lasting 1 hour.

[0016] Preferably, in step a), the relative density of the clear paste at 75-80°C is 1.06, the alcohol content of the predetermined concentration is 60%, and the relative density of the thick paste at 75-80°C is 1.30.

[0017] Preferably, in step b), the glacial acetic acid is used to promote the dissolution of N,O-carboxymethylated chitosan.

[0018] Preferably, in step c), the heating temperature is 40-50°C.

[0019] Preferably, in step d), the predetermined pH value range is 6.0 ± 0.5.

[0020] Preferably, in step e), the medical container is a medical polyethylene injector.

[0021] The targeted solution provided by this invention has the following beneficial effects:

[0022] 1. The hydrogel formulation used in this invention, with its unique three-dimensional network structure and good elasticity, can closely adhere to the surface of the vaginal mucosa, achieving more uniform coating and longer retention. This not only reduces the loss of active ingredients due to the vagina's self-cleaning function, but also ensures that the drug can continuously act on the inflamed area, thereby significantly improving the treatment effect and drug utilization rate.

[0023] 2. The hydrogel prepared by this invention is soft and highly biocompatible, and has almost no irritation or foreign body sensation after use. The drug delivery process is smoother and gentler. This comfortable user experience helps to reduce the psychological burden on patients, enhance their trust and cooperation with the treatment plan, and thus improve medication adherence, creating favorable conditions for continuous treatment and complete recovery of inflammation.

[0024] 3. The matrix material selected in this invention is natural and safe, with good compatibility with human tissues, and will not cause toxic side effects or immune rejection reactions. The hydrogel forms a gentle moisturizing environment in the vagina, which helps to soothe damaged mucosa and promote tissue repair and self-healing. At the same time, its stable drug delivery system can continuously release the active ingredients of traditional Chinese medicine, which can help repair the vaginal environment while reducing inflammation and killing bacteria, achieving the dual effects of treatment and protection, and further improving the safety and overall effect of treatment. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Example 1

[0027] This invention provides a technical solution: a herbal gynecological anti-inflammatory hydrogel, which is composed of the following components by mass percentage: Chinese herbal extract: 14%, matrix: 47.5%, matrix: 38.5%, matrix 1 is N,O-carboxymethylated chitosan, and matrix 2 is L-leucine.

[0028] The herbal extract is prepared by water extraction, alcohol precipitation, and concentration of Chinese medicinal materials including Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla.

[0029] The components and weight parts of the Chinese medicinal materials used to prepare the Chinese herbal extract are as follows: 600-700 parts of Sophora flavescens, 300-350 parts of Stemona japonica, 300-350 parts of Cnidium monnieri, 300-350 parts of Agrimonia pilosa, and 300-350 parts of Callicarpa macrophylla.

[0030] One method for preparing a herbal gynecological anti-inflammatory gel includes the following steps:

[0031] a) Preparation of Chinese herbal extract: Take Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla, add water and decoct, combine the decoctions, filter, and concentrate to obtain a clear extract; add ethanol to the clear extract to make the alcohol content reach the predetermined concentration, let stand, take the supernatant, filter, recover the ethanol and concentrate to obtain a thick extract, which is used as the Chinese herbal extract for later use.

[0032] b) Preparation of matrix 1 solution: N,O-carboxymethylated chitosan was added to purified water and stirred. Glacial acetic acid was added and stirring was continued until completely dissolved to obtain solvent ①.

[0033] c) Preparation of matrix 2 solution: L-leucine was added to purified water and stirred, then heated until completely dissolved to obtain solvent ②;

[0034] d) Mixing and preparing gel: Slowly add the Chinese herbal extract obtained in step a) to the solvent ① obtained in step b) while stirring. After mixing evenly, add the solvent ② obtained in step c) and stir until evenly mixed. Adjust the pH value to the predetermined range to obtain the gel solution.

[0035] e) Filling and sterilization: Fill the gel solution obtained in step d) into a medical container and sterilize it to obtain the final product.

[0036] In step a), the process of adding water and decocting involves three decoctions: the first and second decoctions are each 2 hours long, and the third decoction is 1 hour long.

[0037] In step a), the relative density of the clear paste at 75-80℃ is 1.06, the alcohol content of the predetermined concentration is 60%, and the relative density of the thick paste at 75-80℃ is 1.30.

[0038] In step b), glacial acetic acid is used to promote the dissolution of N,O-carboxymethylated chitosan.

[0039] In step c), the heating temperature is 40-50℃.

[0040] In step d), the predetermined pH range is 6.0 ± 0.5.

[0041] In step e), the medical container is a medical polyethylene injector.

[0042] Example 2

[0043] A herbal gynecological anti-inflammatory gel for treating vaginal inflammation, the components of which are as follows by mass percentage: herbal extract: 14%, N,O-carboxymethylated chitosan: 47.5%, L-leucine: 38.5%;

[0044] The preparation method of a herbal gynecological anti-inflammatory gel for treating vaginal inflammation is as follows:

[0045] A1. Take 600g of Sophora flavescens, 300g of Stemona japonica, 300g of Cnidium monnieri, 300g of Agrimonia pilosa, and 300g of Callicarpa macrophylla. Add water and decoct three times, for 2 hours each time in the first and second decoctions, and 1 hour in the third decoction. Combine the decoctions, filter, and concentrate the filtrate to a clear extract with a relative density of 1.06 at 75℃. Add ethanol to make the alcohol content reach 60%, let stand for 24 hours, take the supernatant, filter, recover the ethanol from the filtrate, and continue to concentrate to a thick extract with a relative density of 1.30 at 75℃ for later use.

[0046] A2. Take 47.5g of N,O-carboxymethylated chitosan and add it to an appropriate amount of purified water. Stir to disperse, add an appropriate amount of glacial acetic acid, and continue stirring until completely dissolved to obtain solution ①.

[0047] A3. Take 38.5g of L-leucine and add it to an appropriate amount of purified water. Stir and heat at 45℃ until completely dissolved to obtain solution ②.

[0048] A4. Slowly add the thick paste obtained in step 1, equivalent to 14g of extract, to solution ① while stirring. After mixing evenly, add solution ② and continue stirring until evenly mixed. Adjust the pH to 6.0 to obtain a gel solution.

[0049] A5. Fill the gel solution into a medical polyethylene syringe and sterilize it by moist heat to obtain the finished product.

[0050] Example 3

[0051] A herbal gynecological anti-inflammatory gel for treating vaginal inflammation, the components of which are as follows by mass percentage: herbal extract: 14%, N,O-carboxymethylated chitosan: 47.5%, L-leucine: 38.5%;

[0052] The preparation method of a herbal gynecological anti-inflammatory gel for treating vaginal inflammation is as follows:

[0053] B1. Take 650g of Sophora flavescens, 325g of Stemona japonica, 325g of Cnidium monnieri, 325g of Agrimonia pilosa, and 325g of Callicarpa macrophylla. Decoction, extraction, and concentration are carried out according to the method in Example 1 to prepare a thick paste for later use.

[0054] B2. Take 47.5g of N,O-carboxymethylated chitosan, add purified water and stir, then add glacial acetic acid to dissolve completely to obtain solution ①;

[0055] B3. Take 38.5g of L-leucine and add it to purified water. Heat and stir at 50℃ until completely dissolved to obtain solution ②.

[0056] B4. Slowly add the thick paste to solution ①, stir until well mixed, then add solution ②, continue stirring until uniform, adjust the pH to 6.5, and obtain the gel solution;

[0057] B5. Filling and sterilization yield the finished product.

[0058] Example 4

[0059] A herbal gynecological anti-inflammatory gel for treating vaginal inflammation, the components of which are as follows by mass percentage: herbal extract: 14%, N,O-carboxymethylated chitosan: 47.5%, L-leucine: 38.5%;

[0060] The preparation method of a herbal gynecological anti-inflammatory gel for treating vaginal inflammation is as follows:

[0061] C1. Take 700g of Sophora flavescens, 350g of Stemona japonica, 350g of Cnidium monnieri, 350g of Agrimonia pilosa, and 350g of Callicarpa macrophylla, extract and concentrate them to a thick paste with a relative density of 1.30 at 80℃ according to the method in Example 1.

[0062] C2. Take 47.5g of N,O-carboxymethylated chitosan, add an appropriate amount of purified water, add glacial acetic acid and stir until completely dissolved to obtain solution ①;

[0063] C3. Take 38.5g of L-leucine, add purified water, heat and stir at 40℃ until completely dissolved to obtain solution ②;

[0064] C4. Slowly add the thick paste to solution ①, stir and mix well, then add solution ②, continue stirring until well mixed, adjust the pH to 5.8, and obtain the gel solution;

[0065] C5. Fill into a syringe and sterilize to obtain the finished product.

[0066] Comparative Example 1

[0067] Comparison with traditional suppositories:

[0068] Comparative Example 1: Traditional Herbal Gynecological Suppository;

[0069] Composition: The same Chinese herbal extracts as in this formulation, but in suppository form, such as an oil-based base;

[0070] Preparation method: Prepared according to the original suppository process;

[0071] Objective: To verify the improvements of hydrogel in terms of vaginal adhesion, drug retention time, and comfort.

[0072] Comparative Example 2

[0073] Comparison of different hydrogel matrices:

[0074] Comparative Example 2: Using conventional hydrogel matrices, such as carbomer and sodium alginate;

[0075] Composition: Traditional Chinese medicine extract + conventional hydrogel matrix;

[0076] Preparation method: similar process;

[0077] Objective of the comparison: To highlight the superiority of N,O-carboxymethylated chitosan over L-leucine in terms of bioadhesion, pH adaptability, and mucosal repair.

[0078] Comparative Example 3

[0079] Comparison of content of different Chinese herbal extracts:

[0080] Comparative Example 3: Hydrogels with low or high content of traditional Chinese medicine extract;

[0081] Composition 1: 8% Chinese herbal extract, the rest are the same as in this formula;

[0082] Composition 2: 20% Chinese herbal extract, the rest are the same as in this formula;

[0083] Objective of the comparison: To verify the optimal ratio of 14% Chinese herbal extract in this scheme, and to balance between efficacy and formulation stability.

[0084] Comparative Example 4

[0085] Comparison without L-leucine:

[0086] Comparative Example 4: Using only N,O-carboxymethylated chitosan as the matrix;

[0087] Composition: 14% Chinese herbal extract + 86% N,O-carboxymethylated chitosan;

[0088] Objective: To verify the role of L-leucine in improving gel elasticity, drug release rate, and comfort.

[0089] Comparative Example 5

[0090] Comparison of different pH adjustments:

[0091] Comparative Example 5: Hydrogels with pH adjusted to 4.0 or 8.0;

[0092] The preparation method is the same, only the final pH value is adjusted;

[0093] Objective: To verify the mildness of pH 6.0 ± 0.5 on the vaginal mucosa and the importance of drug stability.

[0094] Experimental Example 1

[0095] Validation of preparation process and formulation composition:

[0096] Objective: To verify the feasibility of the preparation method of the hydrogel formulation of the present invention and to confirm that the mass percentage of each component meets the design requirements.

[0097] Method: Three batches of hydrogel samples were prepared according to the preparation steps in the invention. The content of each component in each batch of samples was determined, the mass percentage of each component was calculated, and the results were compared with the design values.

[0098] Table: Verification of the mass percentage of components in Baicao Fuyan Qingshui Gel (n=3):

[0099]

[0100]

[0101] Conclusion: The composition of the three batches of samples was basically consistent with the design values ​​(14%, 47.5%, and 38.5%), indicating that the preparation process was stable and feasible.

[0102] Experimental Example 2

[0103] Evaluation of physicochemical properties and adhesive performance:

[0104] Objective: To evaluate the key physicochemical properties of hydrogels, such as pH, viscosity, and adhesion, and to verify their mucosal adhesion performance.

[0105] Methods: The pH value of the hydrogel samples was determined, the viscosity was measured using a rotational viscometer, and the adhesion force was measured using an in vitro adhesion force measuring device to simulate the vaginal mucosal environment.

[0106] Table: Physicochemical Properties and Adhesion Performance of Baicao Fuyan Qing Hydrogel (n=3)

[0107]

[0108] Conclusion: The hydrogel has a pH value that meets the requirements of the vaginal physiological environment, good adhesion, and good mucosal retention capacity.

[0109] Experimental Example 3

[0110] Preliminary evaluation of antibacterial effect:

[0111] Objective: To preliminarily evaluate the antibacterial effect of hydrogel on common vaginitis pathogens, such as Candida albicans and Escherichia coli.

[0112] Methods: The agar diffusion method was used. Hydrogel samples were placed on agar plates inoculated with pathogens and cultured for 24 hours. The diameter of the inhibition zone was measured and compared with that of commercially available herbal gynecological suppositories.

[0113] Table: Preliminary evaluation of the antibacterial effect of Baicao Fuyan Qingli Hydrogel (n=3)

[0114]

[0115] Conclusion: The hydrogel of this invention exhibits superior antibacterial effects against both Candida albicans and Escherichia coli compared to commercially available suppositories, preliminarily confirming its good antibacterial activity.

[0116] Features of this invention: This solution addresses the problems of poor adhesion, easy detachment, and insufficient absorption of active pharmaceutical ingredients in traditional vaginal suppositories such as Baicao Fuyanqing suppositories in clinical applications. It innovatively improves upon these issues by using a hydrogel formulation. The hydrogel is composed of natural or synthetic polymers with a three-dimensional network structure, exhibiting good elasticity, biocompatibility, and mucosal adhesion. It can closely adhere to the vaginal wall, prolonging drug retention time and improving the absorption efficiency of active pharmaceutical ingredients. The formulation consists of traditional Chinese medicine extract, N,O-carboxymethylated chitosan, and L-leucine, mixed in a specific mass percentage ratio to ensure a stable gel structure and good biocompatibility. The preparation method includes steps such as decoction extraction of traditional Chinese medicine, concentration, alcohol precipitation, mixing with a matrix, pH adjustment, filling, and sterilization. The process is mature and controllable, suitable for large-scale production. The hydrogel formulation of this solution is comfortable to use, without foreign body sensation, effectively improving patient compliance and treatment efficacy. It is suitable for the treatment of gynecological inflammations such as fungal, bacterial, and trichomonal vaginitis and cervical erosion.

[0117] The formulation of this solution consists of 14% herbal extract, 47.5% N,O-carboxymethylated chitosan, and 38.5% L-leucine by mass percentage, totaling 100%. The herbal extract is derived from traditional Chinese medicines such as Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla. The effective components are extracted through processes such as decoction, concentration, and alcohol precipitation, preserving the core active ingredients of the Baicao Fuyanqing Suppository. Matrix 1 uses N,O-carboxymethylated chitosan, a natural polymer material with high biocompatibility and strong adhesion, which can enhance the adhesion of the gel to the vaginal mucosa. Matrix 2 uses L-leucine, which has good water solubility and stability, and helps to regulate the rheology and shapeability of the gel. The two matrices work synergistically with the herbal extract to form a gel system with suitable viscosity, pH of 6.0±0.5, and bioadhesive properties, which not only ensures slow drug release and long-lasting effect, but also reduces drug loss due to vaginal self-cleaning.

[0118] The preparation process includes five main steps: Step a) is the preparation of the herbal extract, which involves multiple decoctions, filtrations, concentrations, alcohol precipitation, and further concentrations to obtain a thick paste for later use; Steps b) and c) prepare two matrix solutions respectively: N,O-carboxymethylated chitosan dissolved in purified water containing glacial acetic acid, and L-leucine dissolved in heated purified water; Step d) the herbal extract is mixed sequentially with the two matrix solutions, stirred evenly, and the pH is adjusted to 6.0±0.5 to form a homogeneous gel; Step e) the gel is filled into a medical polyethylene syringe and sterilized to obtain the finished product. This method pays attention to process details, such as decoction time, temperature, and pH control, to ensure stable preparation quality. Using a syringe for filling facilitates patient administration, improves ease of use and hygiene safety, and simplifies the traditional suppository molding process, avoiding high-temperature melting or cooling steps, making it more suitable for temperature-sensitive herbal ingredients, while reducing production costs and improving the consistency and reliability of the preparation.

[0119] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A herbal gynecological anti-inflammatory gel, characterized in that, The composition by mass percentage is as follows: herbal extract: 14%, matrix: 47.5%, matrix: 38.5%, wherein matrix 1 is N,O-carboxymethylated chitosan and matrix 2 is L-leucine.

2. The herbal gynecological cleansing gel according to claim 1, characterized in that: The herbal extract is prepared by water extraction, alcohol precipitation, and concentration of Chinese medicinal materials including Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla.

3. The herbal gynecological cleansing gel according to claim 2, characterized in that: The medicinal materials and their weight parts for preparing the herbal extract are as follows: 600-700 parts of Sophora flavescens, 300-350 parts of Stemona japonica, 300-350 parts of Cnidium monnieri, 300-350 parts of Agrimonia pilosa, and 300-350 parts of Callicarpa macrophylla.

4. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to any one of claims 1-3, characterized in that: Includes the following steps: a) Preparation of Chinese herbal extract: Take Sophora flavescens, Stemona japonica, Cnidium monnieri, Agrimonia pilosa, and Callicarpa macrophylla, add water and decoct, combine the decoctions, filter, and concentrate to obtain a clear extract; add ethanol to the clear extract to make the alcohol content reach the predetermined concentration, let stand, take the supernatant, filter, recover the ethanol and concentrate to obtain a thick extract, which is used as the Chinese herbal extract for later use. b) Preparation of matrix 1 solution: N,O-carboxymethylated chitosan was added to purified water and stirred. Glacial acetic acid was added and stirring was continued until completely dissolved to obtain solvent ①. c) Preparation of matrix 2 solution: L-leucine was added to purified water and stirred, then heated until completely dissolved to obtain solvent ②; d) Mixing and preparing gel: Slowly add the Chinese herbal extract obtained in step a) to the solvent ① obtained in step b) while stirring. After mixing evenly, add the solvent ② obtained in step c) and stir until evenly mixed. Adjust the pH value to the predetermined range to obtain the gel solution. e) Filling and sterilization: Fill the gel solution obtained in step d) into a medical container and sterilize it to obtain the final product.

5. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step a), the boiling process involves boiling the ingredients in water three times, with the first and second boilings each lasting 2 hours, and the third boiling lasting 1 hour.

6. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step a), the relative density of the clear paste at 75-80°C is 1.06, the alcohol content of the predetermined concentration is 60%, and the relative density of the thick paste at 75-80°C is 1.

30.

7. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step b), the glacial acetic acid is used to promote the dissolution of N,O-carboxymethylated chitosan.

8. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step c), the heating temperature is 40-50℃.

9. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step d), the predetermined pH range is 6.0 ± 0.

5.

10. The preparation method of the herbal gynecological anti-inflammatory hydrogel according to claim 4, characterized in that: In step e), the medical container is a medical polyethylene injector.

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