Preparation method of elastin peptide and application of elastin peptide in improvement of skin elasticity

Abstract

The invention discloses a preparation method of elastin peptide and application of the elastin peptide in improvement of skin elasticity, and relates to the technical field of protein peptide compositions prepared by an enzyme catalysis method. According to the invention, through scientific proportioning of multiple active components, the limitation of single component action is broken through, and multi-dimensional synergistic interaction such as supplementing elastic fibers and strengthening structural stability is realized. And the elastin peptide is subjected to enzymolysis optimization through a compound enzyme, the molecular weight meets the absorption requirement, and the penetration auxiliary components are matched, so that active molecules are assisted to play a role more easily. Compared with the prior art, the safety and the efficacy stability are better, potential stimulation of chemical synthesis components is avoided, inactivation of active components is reduced through component protection and process optimization, and the use safety and the efficacy persistence are guaranteed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of protein peptide compositions prepared by using enzyme-catalyzed methods, and in particular to a preparation method of elastin peptides and application thereof in improving skin elasticity. BACKGROUND

[0002] With the increase of age, environmental stimulation and bad living habits, the elastic fibers and collagen in the dermis of the skin are prone to degradation and loss, leading to problems such as decreased skin elasticity and wrinkle growth, which have become the core skin problems that consumers are concerned about at present. The maintenance of skin elasticity depends on the synergistic effect of elastin and collagen. Elastin constitutes the core framework of the skin elastic network, and collagen fills the gaps and enhances the structural stability. The dynamic balance of the two is the key to keeping the skin firm and elastic.

[0003] At present, there are various skin care products and dietary supplements on the market to improve skin elasticity, but most of the products have the problems of single ingredient and one-sided action mechanism. Some products only add single collagen peptide or elastin peptide, which is difficult to achieve synergistic effect of the two proteins. Some products use protein peptides with too large molecular weight, which have low skin absorption rate and cannot effectively penetrate into the dermis to play a role. At the same time, some products add chemical synthetic ingredients, which may irritate the skin and have safety hazards if used for a long time.

[0004] The complex application of plant extracts and bioactive ingredients is a research hotspot in the industry, but the ingredient combination in the existing formula lacks scientific proportioning, and the active ingredients may interact with each other, affecting the efficacy. In addition, there is still room for optimization in the preparation process of elastin peptides. Traditional enzymatic methods mostly use single enzyme preparation, which has low enzymatic efficiency and insufficient product activity. In addition, impurities are easily introduced during the preparation process, affecting the purity and safety of the product.

[0005] Therefore, it is an urgent need to develop an elastin peptide composition with synergistic ingredients, scientific proportioning, good absorption and high safety, which can repair the skin elastic fiber network and supplement collagen through the synergistic effect of multiple ingredients, to solve the problem of decreased skin elasticity. It also provides a new direction for the research and development of related skin care and health products. SUMMARY

[0006] In view of the problems of single ingredient, poor synergistic effect, low absorption and utilization rate of protein peptides, insufficient preparation efficiency of elastin peptides and safety hazards of products in the existing skin elasticity improvement products, a kind of elastin peptide composition with improved skin elasticity and its preparation method are provided, which has scientific ingredient proportioning, synergistic effect of multiple active ingredients, efficient preparation process of elastin peptides and high product purity, can effectively repair the skin elastic fiber and supplement the collagen, and has high absorption and safety.

[0007] To achieve the above object, the technical scheme adopted by the present application is: A preparation method of elastin peptide, comprising the following steps: S1: taking bovine ligament or fish skin elastin tissue, after removing impurities, defatting and crushing, adding into a eutectic solvent, swelling and fiber loosening treatment under the assistance of ultrasonic wave, washing to neutral after centrifugal separation, obtaining pretreated elastin substrate; S2: dispersing the pretreated elastin substrate in water, first adding elastase for site-specific cutting, enzymolysis for 2-3 hours, directly adding a compound of flavor protease and alkaline protease without enzyme inactivation for hydrolysis, applying variable frequency ultrasonic wave treatment in the whole process of enzymolysis; S3: after enzyme inactivation and centrifugal removal of precipitate, the supernatant is sequentially subjected to ceramic membrane microfiltration and ultrafiltration membrane system interception, collecting components with molecular weight less than 1000 Da, and vacuum freeze-drying to obtain elastin peptide powder; The eutectic solvent is formed by stirring choline chloride and urea at a molar ratio of 1:2 at 80 DEG C; The ultrasonic wave assisted treatment in S1 is under the conditions of ultrasonic power 300-500 W, temperature 40-50 DEG C and treatment time 20-40 minutes; In S2, the frequency of variable frequency ultrasonic wave is periodically changed between 20 kHz and 45 kHz, and the alternating period is 5-10 seconds; The adding amount of elastase is 0.5%-1.0% of the substrate mass, the enzymolysis pH value is 8.0-8.5, and the temperature is 50 DEG C; The mass ratio of flavor protease to alkaline protease is 2:1, and the total adding amount is 1.0%-1.5% of the substrate mass, and the second stage enzymolysis is carried out for 3-4 hours.

[0008] By adopting the above technical scheme, further, the elastin peptide prepared by the above preparation method is applied in improving skin elasticity food.

[0009] An improving skin elasticity food, the raw materials of the food comprise, by mass fraction: elastin peptide 10-25 parts, collagen peptide 5-18 parts, sodium hyaluronate 2-8 parts, vitamin composition 0.5-3 parts, trehalose 3-10 parts, improving skin active compound 0.5-1.5 parts, plant extract 0.5-1.5 parts, ceramide 1-5 parts, panthenol 0.3-2 parts; The elastin peptide is prepared by the above preparation method; The improving skin active compound is a compound shown in formula 1; Formula 1; R1 in formula 1 is: methyl, methoxy, nitro, fluorine, hydroxyl.

[0010] Further, the skin active compound is a compound shown in the following structure. The skin active compound 1 is improved. The skin active compound 2 is improved. The skin active compound 3 is improved. The skin active compound 4 is improved. The skin active compound 5 is improved.

[0011] Further, the vitamin composition is a mixture of vitamin E and vitamin C, and the mass ratio of the two is 1:1.5.

[0012] Further, the plant extract is a combined extract of sea fennel and centella asiatica. The preparation method of the combined extract of sea fennel and centella asiatica is as follows: fresh sea fennel and centella asiatica are cut into pieces with a length of 0.5-1.0 cm, 8-12 times the mass of 95% ethanol is added, and ultrasonic extraction is performed at 50°C for 2 hours. After filtration and centrifugation at 800 rpm for 5 minutes, the supernatant is collected, and ultrasonic extraction is repeated three times. The supernatants are combined, concentrated, and dried to obtain the combined extract of sea fennel and centella asiatica.

[0013] Further, the mass ratio of the fresh sea fennel and centella asiatica is 1:1.

[0014] Further, the collagen peptide is selected from any one of deep-sea pollock skin collagen peptide, tilapia skin collagen peptide, and salmon collagen peptide.

[0015] Further, the ubiquinol is D-ubiquinol.

[0016] Further, the preparation method of the skin elasticity improving food comprises the following steps: the elastin peptide, collagen peptide, sodium hyaluronate, vitamin composition, trehalose, skin active compound, plant extract, ceramide, and ubiquinol are added to a mixing machine, mixed at 25-35°C and a rotation speed of 150-200 r / min for 15-25 min, and sieved through a 80-120 mesh sieve to obtain the skin elasticity improving food.

[0017] By adopting the above technical scheme, further, after sieving, a sterilization step is further included, ultraviolet sterilization is adopted, the sterilization time is 20-30 min, the ultraviolet wavelength is 254 nm, and after sterilization, the sub-packaging is carried out in a sterile environment.

[0018] The component design and preparation process of the present application are not simply raw material stacking or step combination, but a scientific scheme formed through systematic research based on the core needs of the target function, combined with the basic principles and practical experience of the present application, and the core mechanism can be summarized as follows: As a food, the quality part formula is accurately matched and functionally synergized through multiple components, from four dimensions of core component supplementation, absorption efficiency improvement, activity protection enhancement and safety risk avoidance, to solve the technical problems of "single component synergy, low protein and peptide absorption and utilization rate, activity loss and safety risk" of existing skin elasticity improving food. Among them, the elastin peptide and the collagen peptide are compounded in an optimized ratio, the former is the skeleton component of the skin elasticity network, which can directly supplement the loss of dermal elastic fiber, and the latter fills the gap between the elastic fibers and enhances the structural stability, the two synergistically break the limitation of single peptide which can only act on a certain link, and solve the core problem of "single component and poor synergy"; the elastin peptide is prepared by complex enzyme hydrolysis of neutral protease and papain, the molecular weight is more suitable for the absorption threshold of human intestinal tract, and the penetration auxiliary effect of sodium hyaluronate can promote the penetration of peptide active molecules through the intestinal mucosal barrier, and greatly improve the absorption and utilization rate; in the vitamin composition, vitamin C can activate collagen synthetase, and vitamin E can inhibit the oxidative degradation of elastin, and the two can synergistically enhance the efficacy of core peptides, while trehalose can wrap active ingredients such as elastin peptides and vitamins in food processing and storage to prevent inactivation or degradation and ensure the stability of efficacy; the combination of skin active compounds, ceramides, panthenol and the combined extract of sea fennel and asiatic pennywort can not only promote skin cell renewal through skin active compounds and ceramide supplement skin barrier lipids, but also rely on the anti-inflammatory and antioxidant properties of plant extracts to reduce the damage of environmental factors to skin elasticity. Ultimately, through multi-dimensional synergy, the effects of supplementing elastic fiber raw materials, promoting absorption and utilization, enhancing efficacy stability and ensuring food safety are achieved.

[0019] Compared with the prior art, the present application has the following advantages: 1. Stronger component synergy, through scientific matching of multiple active ingredients, breaking the limitation of single component action, realizing multi-dimensional synergistic effect of supplementing elastic fiber and strengthening structural stability.

[0020] 2. Higher absorption and utilization rate, the elastin peptide is optimized by complex enzyme hydrolysis, the molecular weight is matched with the absorption requirement, and the penetration auxiliary ingredient is matched to help the active molecules to play a role more easily.

[0021] 3. The safety and efficacy stability are better, the potential stimulation of chemical synthesis components is avoided, the active ingredient inactivation is reduced through component protection and optimization process, and the use safety and efficacy sustainability are ensured.

[0022] The above description is only a summary of the technical solutions of the present application. In order to make the technical means of the present application more clear and understandable, and to make the above and other purposes, characteristics and advantages of the present application more obvious and easy to understand, the following specific embodiments of the present application are described. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the present application, the drawings used in the present application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0024] Figure 1 NMR chart of the skin-activating compound 1 improved by the present application.

[0025] Figure 2 NMR chart of the compound 3 involved in the embodiment 1 of the present application.

[0026] Figure 3 NMR chart of the skin-activating compound 2 improved by the present application.

[0027] Figure 4 NMR chart of the skin-activating compound 3 improved by the present application.

[0028] Figure 5 NMR chart of the skin-activating compound 4 improved by the present application.

[0029] Figure 6 NMR chart of the skin-activating compound 5 improved by the present application. DETAILED DESCRIPTION

[0030] The technical solutions of the present application will be described clearly and completely below in combination with the drawings in the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0031] Some specific examples and preparation examples are listed below. It should be noted that the examples and preparation examples described below are exemplary and are only used to explain the present application and cannot be understood as a limitation of the present application. If a specific technology or condition is not specified in the examples and preparation examples, it is carried out according to the technology or condition described in the literature in the art or according to the product manual. If the manufacturer of the reagent or instrument is not specified, it is a conventional product that can be obtained on the market.

[0032] Preparation Example 1 Preparation of skin-activating compound 1: ; Compound 1: ethyl (2Z,4E,6E,8E)-2-(chlorocarbonyl)-3,7-dimethyl-9-(2,6,6- trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraenoate; Compound 2: 3-(bromomethyl)-2,4,4-trimethylcyclohexen-1-ol; Compound 3: 1-(3-(bromomethyl)-2,4,4-trimethylcyclohex-2-en-1-yl) 3-ethyl (Z)- 2-((3E,5E,7E)-6-methyl-8-(2,6,6-trimethylcyclohex-1-en-1-yl)octa-3,5,7-trien-2- ylidene)maleate; Under a nitrogen atmosphere, 5.18 g of triethylamine, 10.00 g of compound 1 were added to 120 mL of 1,4-dioxane, and after stirring to homogeneity, 5.96 g of raw material 2 was dissolved in 20 mL of 1,4-dioxane. The temperature of the system was reduced to -10°C, and the temperature was kept below -10°C while slowly adding the above solution dropwise. After the addition was complete, the system was stirred at 40°C for 12 h. After the reaction was completed, the reaction solution was slowly poured into 500 mL of water at 4°C and stirred for 1 h. The pH of the system was adjusted to neutral with 0.1 mol / L hydrochloric acid, the organic phase was separated, the organic phase was concentrated, and the product was purified by silica gel column chromatography using a mixture of petroleum ether and ethyl acetate as the eluent. The residue was dried under vacuum to obtain 12.12 g of compound 3; ; Compound 3: 1-(3-(bromomethyl)-2,4,4-trimethylcyclohex-2-en-1-yl) 3-ethyl (Z)- 2-((3E,5E,7E)-6-methyl-8-(2,6,6-trimethylcyclohex-1-en-1-yl)octa-3,5,7-trien-2- ylidene)maleate; Compound 4: N-(p-tolyl)acetamide; Skin-improving active compound 1: 1-Ethyl-3-(2,4,4-trimethyl-3-((N-(p-tolyl)acetamido)methyl)cyclohexyl-2-en-1-yl)(Z)-2-((3E,5E,7E)-6-methyl-8-(2,6,6-trimethylcyclohexyl-1-en-1-yl)oct-3,5,7-trien-2-yl)maleic acid diester; Under a nitrogen atmosphere, 12.12 g of compound 3, 3.08 g of compound 4, and 150 ml of toluene were added to a reaction vessel and stirred until homogeneous. The air was then replaced with nitrogen twice. 4.17 g of triethylamine, 0.13 g of palladium acetate, and 0.16 g of triphenylphosphine were added sequentially, and the mixture was heated to 120 °C and refluxed for 12 hours. After the reaction was completed, the solvent was evaporated, and silica gel column chromatography was performed using a mixture of petroleum ether and ethyl acetate as eluent. The solvent was evaporated again to obtain 9.81 g of compound 1, which improves skin activity.

[0033] Compound structure identification data: Compound structure identification can rely on 1H NMR spectroscopy (NMR spectroscopy). 1 ¹H NMR, mass spectrometry (MS), and other methods are commonly used core identification methods in this field. For those skilled in the art, given a clear understanding of the raw material composition, reaction conditions, and raw material ratios, combining any one of the above-mentioned compound structure identification data is sufficient to form a clear and accurate judgment on the specific chemical structure of the final product. Mass spectrum of compound 3: 587 [M+1]; Mass spectrometry of skin-enhancing compound 1: 656 [M+1]; The NMR spectra of compound 1 that improves skin activity are shown in [reference needed]. Figure 1 .

[0034] Preparation Examples 2-5 In Preparation Examples 2-5, skin-improving active compound 2-skin-improving active compound 5 were prepared sequentially, following the preparation method of Preparation Example 1, except that compound 4 was replaced, and the rest remained the same as in Preparation Example 1. See Table 1 for details.

[0035] Table 1

[0036] Example 1 Preparation of a food product that improves skin elasticity: 1. Preparation of an elastin peptide: S1. Take bovine ligamentum nuchae elastin tissue, remove connective tissue and impurities by physical impurity removal, remove fat components after defatting treatment, crush to 50-80 mesh powder, add eutectic solvent (the eutectic solvent is formed by stirring choline chloride and urea at a molar ratio of 1:2 at 80°C for 30 min to form a uniform transparent solution), and place it in an ultrasonic reaction device for swelling and fiber loosening treatment; the conditions of the ultrasonic assisted treatment are: ultrasonic power 400W, temperature 45°C, treatment time 30 minutes; after the treatment is completed, centrifuge at 8000 rpm for 15 min, collect the precipitate, wash repeatedly with deionized water until the pH of the washing liquid is 7.0, and obtain a pretreated elastin substrate; S2. Disperse the pretreated elastin substrate in deionized water at a solid-liquid ratio of 1:20 (g / mL), stir uniformly, then add 0.8% elastase based on the mass of the substrate for site-specific cutting, adjust the pH of the system to 8.2, control the temperature at 50°C, and hydrolyze for 2.5 hours; after the set time, add a compound of flavor protease and alkaline protease (the mass ratio of flavor protease to alkaline protease is 2:1, and the total addition amount is 1.2% of the mass of the substrate) to inactivate the enzyme, and perform the second stage of hydrolysis for 3.5 hours; during the entire enzymatic hydrolysis process, variable frequency ultrasonic treatment is applied, and the frequency of the variable frequency ultrasonic waves is periodically alternated between 20 kHz and 45 kHz, with an alternating period of 8 seconds; S3. After the enzymatic hydrolysis is completed, the enzyme solution is placed in a 90°C water bath for 20 min to inactivate the enzyme, cooled to room temperature, and centrifuged at 10000 rpm for 20 min to remove insoluble precipitate; collect the supernatant, first remove impurities by microfiltration through a ceramic membrane with a pore size of 0.2 μm, then pass the microfiltration permeate into an ultrafiltration membrane system with a molecular weight cutoff of 1000 Da for cutoff separation, and collect the ultrafiltration permeate; place the ultrafiltration permeate in a vacuum freeze dryer and freeze dry at -50°C under a vacuum of 0.08 MPa for 24 hours to obtain an elastin peptide powder.

[0037] 2. Mass distribution formula: Elastin peptide: 18 parts; Collagen peptide: 12 parts (deep sea cod skin collagen peptide is selected, purchased from Shandong Longbei Biological Technology Co., Ltd.); Sodium hyaluronate: 5 parts; Vitamin composition: 1.5 parts (vitamin E 0.6 parts, vitamin C 0.9 parts, mass ratio 1:1.5); Trehalose: 6 parts; Skin improvement active compound: 1.0 part (skin improvement active compound 1 prepared in Preparation Example 1 is selected); Plant extract: 1.0 part (combined extract of sea fennel and gotu kola); Ceramide: 3 parts; Ubiquinol: 1.0 part (D-ubiquinol); The preparation method of the combined extract of sea fennel and centella asiatica is as follows: fresh sea fennel and centella asiatica (mass ratio 1:1) are cut into pieces with a length of 0.8 cm, 10 times the mass of 95% ethanol is added, and ultrasonic extraction is performed at 50°C for 2 hours; after filtration, centrifugation is performed at 800 rpm for 5 minutes, and the supernatant is taken; the above ultrasonic extraction-filtering-centrifugation operation is repeated 3 times, and all the supernatants are combined; the combined supernatant is placed in a rotary evaporator, and concentrated under reduced pressure to 1 / 5-1 / 4 of the original volume under the conditions of vacuum degree 0.08-0.10 mbar and 45°C; the concentrate is quickly transferred to the cold trap of a freeze dryer, pre-frozen at -40 to -45°C for 2.5 hours, and then the freeze dryer parameters are adjusted: vacuum degree 0.1 mbar, shelf temperature -50°C, and maintained for 28 hours until the water content of the extract is ≤3%; then it is taken out, crushed through an 80-mesh sieve, and the combined extract of sea fennel and centella asiatica is obtained.

[0038] 3. Preparation method: the elastin peptide, deep-sea pollock skin collagen peptide, sodium hyaluronate, vitamin composition, trehalose, skin improvement active compound 1, sea fennel and centella asiatica combined extract, ceramide and D-ubiquinol in the above formula amount are sequentially added into a double-helical conical mixer, the mixing temperature is set to 30°C, the rotation speed is 180 r / min, and the mixing time is 20 min; after mixing, the material is sieved through a 100-mesh standard sieve to remove large particle impurities and agglomerates; then the sieved material is placed in an ultraviolet sterilization box and sterilized under ultraviolet wavelength 254 nm for 25 min; after sterilization, it is quickly transferred to a sterile operating room, packaged according to the specification of 5 g per bag, and sealed to obtain the skin elasticity improvement food.

[0039] Examples 2-5 A skin elasticity improvement food is prepared according to the preparation method of Example 1, wherein the skin improvement active compound 1 is replaced by skin improvement active compounds 2-5 in sequence, and the rest remains the same as Example 1.

[0040] Comparative Example 1 A skin elasticity improvement food is prepared according to the preparation method of Example 1, wherein the skin improvement active compound 1 is replaced by retinol ( ), and the rest remains the same as Example 1.

[0041] Comparative Example 2 A skin elasticity improvement food is prepared according to the preparation method of Example 1, wherein the skin improvement active compound 1 is replaced by astaxanthin, and the rest remains the same as Example 1.

[0042] Comparative Example 3 A preparation of a skin elasticity improving food, referring to the preparation method of Example 1, the skin elasticity improving active compound 1 therein is replaced by coenzyme Q10 (purchased from: Shenzhen Hijiya Biotechnology Co., Ltd.), and the rest is the same as Example 1.

[0043] Comparative Example 4 A preparation of a skin elasticity improving food, referring to the preparation method of Example 1, the skin elasticity improving active compound 1 therein is not added, and the rest is the same as Example 1.

[0044] Comparative Example 5 A preparation of a skin elasticity improving food, referring to the preparation method of Example 1, the plant extract therein is not added, and the rest is the same as Example 1.

[0045] Comparative Example 6 A preparation of a skin elasticity improving food, referring to the preparation method of Example 1, the preparation method of elastin peptide does not use frequency ultrasonic treatment, only ultrasonic treatment, and the rest is the same as Example 1.

[0046] Performance test: 1. Core model: human dermal fibroblast (HDF) 3D culture model; 2. Experimental grouping: containing 20 mg / mL of the composition of the application; 3. Elastin synthesis amount detection (ELISA method): operation steps: HDF cells are inoculated in 3D culture plates (density 5×10 4 cells / well), after 24h of culture, each group of test solution is added, and the culture is continued for 72h; the culture medium supernatant is collected, human elastin (Elastin) ELISA kit is used, operation is carried out according to the instruction manual, and the absorbance (OD value) is detected at 450nm wavelength, the data is shown in Table 2; 4. Antioxidant capacity detection (DPPH free radical scavenging method): operation steps: 0.1mmol / L DPPH ethanol solution is prepared, 2mL is taken and mixed with 2mL of each group of test solution, and incubated in the dark for 30min; the OD value is detected at 517nm wavelength, the blank group is DPPH solution+ethanol, and the free radical scavenging rate (%)=[1-(experimental group OD value / blank group OD value)]×100%, the data is shown in Table 2.

[0047] Table 2

[0048] The effect of the embodiment group on the promotion of elastin synthesis and the free radical scavenging ability is better than that of the comparative example group as a whole, and the effect of each group using the specific skin improvement active compound of the application is balanced; the comparative examples using traditional retinol, astaxanthin and other ingredients, and the comparative examples without the addition of the skin improvement active compound or the plant extract, show a significant decline in the two indicators, especially when the skin improvement active compound or the plant extract is lacking, the decline in antioxidant capacity is more prominent, indicating that the skin improvement active compound and the plant extract of the application play a key synergistic role in improving the efficacy of the composition. Comparative Example 6 does not use frequency ultrasonic treatment in the preparation process of elastin peptide, only uses single ultrasonic assisted enzymolysis, which leads to the decline of enzymolysis efficiency, the reduction of the uniformity of the molecular weight distribution and the activity of elastin peptide, and further affects its ability to promote human dermal fibroblasts to synthesize elastin, and at the same time weakens the antioxidant synergistic effect of the system, so the two detection indicators are lower than each embodiment.

[0049] Although embodiments of the application have been shown and described, it is to be understood that various modifications, substitutions, replacements and changes can be made to these embodiments without departing from the principles and spirit of the application, and the scope of the application is defined by the appended claims and their equivalents.

Claims

1. A method for preparing an elastin peptide, characterized by, It comprises the following steps: S1: Take bovine nuchal ligament or fish skin elastin tissue, after removing impurities, defatting, crushing, adding into a eutectic solvent, swelling and fiber loosening treatment under the assistance of ultrasonic wave, centrifugal separation, and washing to neutral, to obtain a pretreated elastin substrate; S2: Disperse the pretreated elastin substrate in water, first add elastase for site-specific cutting, enzymolysis for 2-3 hours, without enzyme inactivation, directly add a compound of flavor protease and alkaline protease for hydrolysis, and apply variable frequency ultrasonic wave treatment during the whole enzymolysis process; S3: After enzyme inactivation, centrifugal separation to remove the precipitate, the supernatant is sequentially subjected to ceramic membrane microfiltration and ultrafiltration membrane system interception, collect the component with molecular weight less than 1000 Da, and vacuum freeze-drying to obtain elastin peptide powder; The eutectic solvent is formed by stirring choline chloride and urea at a molar ratio of 1:2 at 80℃; The ultrasonic wave assisted treatment in S1 is under the following conditions: ultrasonic power 300-500 W, temperature 40-50℃, and treatment time 20-40 minutes; In S2, the frequency of variable frequency ultrasonic wave is periodically changed between 20 kHz and 45 kHz, and the alternating period is 5-10 seconds; The adding amount of elastase is 0.5%-1.0% of the substrate mass, the enzymolysis pH value is 8.0-8.5, and the temperature is 50℃; The mass ratio of flavor protease and alkaline protease is 2:1, and the total adding amount is 1.0%-1.5% of the substrate mass, and the second stage enzymolysis lasts for 3-4 hours.

2. Application of elastin peptide prepared by the preparation method of elastin peptide in claim 1 in a food for improving skin elasticity.

3. A food for improving skin elasticity, characterized by, The raw materials of the food include, in mass parts: elastin peptide 10-25 parts, collagen peptide 5-18 parts, sodium hyaluronate 2-8 parts, vitamin composition 0.5-3 parts, trehalose 3-10 parts, skin improvement active compound 0.5-1.5 parts, plant extract 0.5-1.5 parts, ceramide 1-5 parts, panthenol 0.3-2 parts; The elastin peptide is prepared by the preparation method in claim 1; The skin improvement active compound is a compound shown in formula 1; Formula 1; R1 in formula 1 is: methyl, methoxy, nitro, fluorine, hydroxyl.

4. The food for improving skin elasticity according to claim 3, characterized by, The vitamin composition is a mixture of vitamin E and vitamin C, and the mass ratio of the two is 1:1.

5.

5. The food for improving skin elasticity according to claim 3, wherein The plant extract is a combined extract of sea fennel and centella asiatica; The preparation method of the combined extract of sea fennel and centella asiatica is: take fresh sea fennel and centella asiatica, cut into blocks with a length of 0.5-1.0 cm, add 8-12 times the mass of 95% ethanol, extract for 2 hours under ultrasonic extraction in a 50℃ water bath, filter, centrifuge at 800 rpm for 5 minutes, take the supernatant, repeat the ultrasonic extraction for 3 times, combine the supernatant, concentrate, dry to obtain the combined extract of sea fennel and centella asiatica.

6. The food for improving skin elasticity according to claim 5, wherein The mass ratio of the fresh sea fennel and centella asiatica is 1:

1.

7. The food for improving skin elasticity according to claim 3, wherein The collagen peptide is selected from any one of deep-sea pollock skin collagen peptide, tilapia skin collagen peptide, and salmon collagen peptide.

8. The food for improving skin elasticity according to claim 3, characterized by, The panthenol is D-panthenol.

9. The food for improving skin elasticity according to claim 3, characterized by, The preparation method of the skin elasticity improving food comprises the following steps: adding the elastin peptide, the collagen peptide, the sodium hyaluronate, the vitamin composition, the trehalose, the skin activity improving compound, the plant extract, the ceramide and the panthenol into a mixing machine, mixing at 25-35 DEG C and a rotating speed of 150-200 r / min for 15-25 min, screening through a 80-120 mesh screen, and obtaining the skin elasticity improving food.

10. The food for improving skin elasticity according to claim 9, wherein After the screening, a sterilization step is further included, and the sterilization is performed by using ultraviolet sterilization for 20-30 min at a wavelength of 254 nm, and the sterilization is performed in a sterile environment after the sterilization.

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