Rice straw degrading bacterial agent

Abstract

The invention relates to the field of rice straw degradation, in particular to a rice straw degradation fungicide, a preparation method of the fungicide, a method for preparing an organic fertilizer by using the fungicide and application of the fungicide. Compared with the prior art, the rice straw degrading bacterial agent and the application method of the biological organic fertilizer of the rice straw degrading bacterial agent provided by the invention have the advantages that the degrading bacteria burkholderia cepacia and pandoraea have obvious plate effect and field application effect on the rice straw, and the two bacteria have few reports in the aspect of straw degradation; according to the present invention, the degrading bacteria are prepared into the degrading bacteria inoculant, the degrading bacteria inoculant is combined with the fresh compost carrier to perform secondary solid fermentation, and the organic carrier can provide nutrients for the growth of the degrading bacteria, such that the survival and the colonization of the degrading bacteria in the soil are easily achieved, the application effect of the degrading bacteria in straw degradation is ensured, and the degradation efficiency can be significantly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of rice straw degradation, in particular to a rice straw degradation microbial agent. BACKGROUND

[0002] Guangdong Province is a typical double-cropping rice production area in South China, and the amount of straw produced is large, wide, and difficult to handle. Due to the low value of straw industrialization, the current application is more for direct return to the field, but the interval between double-cropping rice in our province is short, generally only 15-20 days, and the straw is not fully degraded, and under natural conditions, the straw is slowly decomposed, and a large amount of straw returned to the field will affect the rooting and survival of crops, and will also cause inconvenience in production management and affect the planting of the next crop. Therefore, it is urgent to develop a rapid straw decomposition technology.

[0003] The method for degrading straw includes physical method, chemical method and biological method. The physical method mainly uses high-temperature thermal degradation, and the chemical method mainly uses acid and alkali reagents and oxidizing agents. These two methods not only consume a lot of energy and cost a lot, but also cause environmental pollution and produce harmful by-products, which is not conducive to sustainable application. The biological method uses bacteria, fungi, actinomycetes and other microorganisms that can produce cellulase to treat the straw, which consumes less energy, costs less and is environmentally friendly, so it has attracted widespread attention and achieved certain results. Fungi have strong ability to produce cellulase and grow faster, and can produce higher specific multi-enzyme complex of actinomycetes such as single cell bacteria and streptomycin. Bacteria have stronger environmental adaptability, and the microorganisms that can degrade cellulose and lignin include bacteria, fungi and pseudomonas. In the straw, lignin and hemicellulose form a network to wrap cellulose, forming a barrier between cellulose and its degrading enzymes, so it is difficult for single-function cellulose-degrading bacteria to effectively degrade straw. Therefore, when screening straw-degrading bacteria, the degradation ability of the strain to cellulose, hemicellulose and lignin should be considered comprehensively, and the strain combination mode should be adopted to improve the degradation efficiency of straw.

[0004] During the application of the microbial agent, a suitable organic carrier needs to be provided to ensure its effective colonization in the soil, so as to better play the corresponding role. The present application combines the rice straw degradation microbial agent with the organic carrier for secondary fermentation to obtain a bio-organic fertilizer product. The product not only provides nutrients for the growth of the degradation bacteria to promote their successful colonization in the soil, but also improves the physical and biological properties of the soil and promotes the growth of plants. SUMMARY

[0005] (I) Technical problems solved The technical problem to be solved by the present application is: The current straw degradation is not sufficient and the effect is poor; The secondary fermentation process of the microbial agent combined with the organic carrier of the present application; The application provides an application method of an organic fertilizer formed after secondary fermentation of a microbial agent.

[0006] (II) Technical solutions To solve the above technical problems, the technical solutions provided by the application are as follows: a rice straw degradation microbial agent, comprising: Berkholderia cepacia and Pandora for rice straw degradation, and LB liquid culture medium.

[0007] The preparation method of the rice straw degradation microbial agent comprises the following steps: inoculating Berkholderia cepacia and Pandora as microbial strains in LB liquid culture medium for culture to obtain a culture solution.

[0008] The bacterial culture in the culture solution is centrifuged and resuspended with deionized water to obtain a liquid microbial agent.

[0009] As an improvement, the microbial agent is in a liquid state, and in the liquid microbial agent, the number of viable bacteria, i.e., Berkholderia cepacia and Pandora, is 1×10 9-10 cfu / mL.

[0010] As an improvement, the incubation parameters for preparing the microbial agent are as follows: the environmental temperature is between 28 ℃ and 32 ℃, and after 10 h-16 h of shaking culture at 150 r / min-190 r / min, the microbial agent is transferred to fresh LB liquid culture medium and is subjected to shaking culture for 60 h-80 h.

[0011] As an improvement, the microbial agent is combined with an organic carrier at a solid-liquid ratio of 5:1, and is subjected to aerobic composting treatment, i.e., secondary fermentation.

[0012] As an improvement, the organic carrier is a cow manure compost product.

[0013] As an improvement, before composting treatment, the microbial agent is inoculated into the organic carrier at a solid-liquid ratio of 5:1, and 10% peanut meal or rapeseed meal is added as an auxiliary material to obtain a raw material mixture; the initial concentration of the degradation bacteria is 1.0×10 9 cfu / mL, and the moisture content of the raw material mixture is adjusted to 50 wt%-60 wt% after inoculation; or Before composting treatment, 10% peanut meal and rapeseed meal are added as auxiliary materials in the organic carrier to obtain a raw material mixture, the moisture content of the raw material mixture is adjusted to 50 wt%-60 wt% to obtain a composting heap, and after the temperature of the heap is reduced to below 35 ℃, the microbial agent is inoculated into the heap at a solid-liquid ratio of 5:1, and the initial concentration of the degradation bacteria is 1.0×10 9 cfu / mL. or Before composting, the microbial inoculant is inoculated into the organic carrier at a solid-liquid ratio of 5:1, 10% peanut meal and rapeseed meal are added as auxiliary materials to obtain a raw material mixture, the initial concentration of the degrading bacteria is 1.0*10 9 After the temperature of the heap is reduced to below 35 DEG C, the microbial inoculant is inoculated into the heap at a solid-liquid ratio of 5:1 again.

[0014] As an improvement, the secondary fermentation is carried out in an intelligent composting reactor. The heap is turned over every 2 days, and ventilated for 6 minutes every hour, with a ventilation volume of 30 L / min. The temperature of the heap is maintained at 35-45 DEG C for more than 5 days, and then the moisture content of the heap is dried to 30 wt%-40 wt% when the temperature is reduced to below 35 DEG C.

[0015] As an improvement, the concentration of the degrading bacteria in the bio-organic fertilizer is 1.0*10 8 cfu / g dry sample; In the field test, the product is applied as base fertilizer before rice transplanting, and the product is applied again after the rice stalks are crushed and returned to the field after 7 days of water soaking.

[0016] (Three), beneficial effects Compared with the prior art, the application has the advantages that: the application provides a rice straw degrading bacteria agent and a bio-organic fertilizer and an application method thereof, the degrading bacteria Burkholderia glumae and Pandora have significant plate effects and field application effects on rice straw, and the two strains are rarely reported in straw degradation, the present application is made into a degrading bacteria agent, combined with fresh compost carrier for secondary solid fermentation, the organic carrier can provide nutrients for the growth of the degrading bacteria, which is conducive to the survival and colonization of the degrading bacteria in the soil, and guarantees the application effect of the degrading bacteria in straw degradation, and can significantly improve the degradation efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is a process schematic diagram of the preparation process of the rice straw degrading bacteria agent.

[0018] Figure 2 is a process schematic diagram of the secondary fermentation of the rice straw degrading bacteria agent to prepare a derivative product. DETAILED DESCRIPTION

[0019] The application will be further described in detail below with reference to the drawings. EMBODIMENT

[0020] To solve the above technical problems, the technical scheme provided by the present application is: a rice straw degrading bacterial agent, comprising: Burkholderia cepacia and Pandoraea apnomenusa for degrading rice straw, and LB liquid medium; A preparation method of the rice straw degrading bacterial agent, comprising: inoculating Burkholderia cepacia and Pandoraea apnomenusa as bacterial strains in LB liquid medium for culture to obtain a culture solution; Centrifuging the bacterial culture in the culture solution and resuspending with deionized water to obtain a liquid bacterial agent; The incubation parameters for preparing the bacterial agent are: the environmental temperature is between 28 ℃ and 32 ℃, and after shaking culture at 150 r / min-190 r / min for 10 h-16 h, transferring to fresh LB liquid medium and then shaking culture for 60 h-80 h; The bacterial agent is in a liquid state, and in the liquid bacterial agent, the number of viable bacteria, i.e., Burkholderia cepacia and Pandoraea apnomenusa, is 1×10 9-10 cfu / mL; The bacterial strains of the bacterial solution are Burkholderia cepacia and Pandoraea apnomenusa, which are preserved in the China General Microbiological Culture Collection Center, located at No. 1, Beichen West Road, Haidian District, Beijing, with preservation numbers of CGMCC NO. 33108 and CGMCC NO. 33004, respectively, and preservation times of December 18, 2024 and December 9, 2024, respectively. Embodiment

[0021] The bacterial agent is mixed with an organic carrier at a solid-liquid ratio of 5:1 for aerobic composting treatment, i.e., secondary fermentation, and the organic carrier is a cow manure compost product; The secondary fermentation is carried out in an intelligent composting reactor, and the pile is turned over every 2 d; ventilation is performed every 1 h for 6 min, and the ventilation volume is 30 L / min; The pile maintains a high temperature of 35-45 ℃ for more than 5 d, and after the temperature drops to below 35 ℃, the pile is dried to a moisture content of 30 wt%-40 wt%; Before composting treatment, the microbial agent is inoculated into the organic carrier at a solid-liquid ratio of 5:1, and 10% peanut meal or rapeseed meal is added as an auxiliary material to obtain a raw material mixture; the initial concentration of the degrading bacteria is 1.0×10 9 cfu / mL, and the moisture of the raw material mixture is adjusted to 50 wt%-60 wt% after inoculation; or Before composting, 10% peanut meal and rapeseed meal are added to the organic carrier as auxiliary materials to obtain a raw material mixture, the moisture of the raw material mixture is adjusted to 50 wt%-60 wt% to obtain a compost heap, after the temperature of the compost heap is reduced to below 35 DEG C, the microbial inoculum is inoculated into the compost heap at a solid-liquid ratio of 5:1, and the initial concentration of the degrading bacteria is 1.0x10 9 cfu / mL; or Before composting, the microbial inoculum is inoculated into the organic carrier at a solid-liquid ratio of 5:1, 10% peanut meal and rapeseed meal are added as auxiliary materials to obtain a raw material mixture, the initial concentration of the degrading bacteria is 1.0x10 9 cfu / mL, and after inoculation, the moisture of the raw material mixture is adjusted to 50 wt%-60 wt% to obtain a compost heap. After the temperature of the compost heap is reduced to below 35 DEG C, the microbial inoculum is again inoculated into the compost heap at a solid-liquid ratio of 5:1.

[0022] The concentration of the degrading bacteria in the bio-organic fertilizer is 1.0x10 8 cfu / g dry sample; In field trials, the product is applied as base fertilizer in the soil before rice transplanting, and at the time of rice harvesting, the rice straw is crushed and returned to the field, after the field is soaked for 7 days, the product is again applied and mixed well with the soil and the rice straw.

[0023] In use, the organic fertilizer prepared based on the microbial inoculum is first applied to the field, the straw recovered from the field is crushed by a crushing machine, and then re-applied to the field, after a certain amount of water is injected into the field to form a 5-10 cm deep water layer, the straw is soaked for 7 days, then drained, and the fertilizer powder is applied, the fertilizer, straw and soil are mixed well by a rotary tiller, and the fertilizer is used as nutrient for the soil; In the production of the organic fertilizer, the Burkholderia cenocepacia and Pandora are inoculated into LB liquid medium as inoculum for culture, and under appropriate environmental conditions, the bacterial liquid is obtained. The bacterial liquid is further mixed with the cow manure compost product, and after secondary fermentation, the product is obtained.

[0024] The above description of the present application and its embodiments is not limiting, and the embodiments shown in the drawings are only one of the embodiments of the present application, and the actual structure is not limited thereto. In summary, if a person skilled in the art is inspired thereby, without departing from the purpose of the present application, without creative design, similar structural modes and embodiments of the technical solutions are not creative, and all should belong to the protection scope of the present application.

Claims

1. A rice straw degrading microbial agent, characterized in that, include: Burkholderia cepacia and Pandora bacilli used for the degradation of rice straw, as well as LB liquid medium; The method for preparing a rice straw degrading microbial agent includes: inoculating Burkholderia cepacia and Pandora bacillus as inoculum in LB liquid medium and culturing them to obtain a culture solution; The bacterial culture in the culture medium was centrifuged and then resuspended in deionized water to obtain a liquid bacterial agent.

2. The rice straw degradation microbial agent according to claim 1, characterized in that: The bacterial agent is in liquid form, and the number of live bacteria, namely Burkholderia cepacia and Pandora bacilli, in this liquid bacterial agent is 1 × 10⁻⁶. 9-10 cfu / mL.

3. The method for preparing a rice straw degrading microbial agent according to claim 2, characterized in that: The cultivation parameters for preparing this bacterial agent are as follows: the ambient temperature is between 28 ℃ and 32 ℃, and the culture is carried out on a shaker at 150 r / min to 190 r / min for 10 h to 16 h, then transferred to fresh LB liquid medium and cultured on a shaker for another 60 h to 80 h.

4. The method for preparing bio-organic fertilizer from rice straw degrading microbial agents according to claim 1, characterized in that: The microbial agent is combined with an organic carrier and mixed at a solid-liquid ratio of 5:1 for aerobic composting, i.e., secondary fermentation.

5. The rice straw degradation microbial agent according to claim 4, characterized in that: The organic carrier is a cow manure compost product.

6. The method for preparing bio-organic fertilizer from rice straw degrading microbial agents according to claim 4, characterized in that: Before composting, the microbial inoculant is inoculated into the organic carrier at a solid-liquid ratio of 5:1, and 10% peanut bran or rapeseed meal is added as an auxiliary material to obtain a raw material mixture; the initial concentration of the degrading bacteria is 1.0 × 10⁻⁶. 9 cfu / mL, after inoculation, adjust the moisture content of the raw material mixture to 50 wt%-60 wt%; or Before composting, 10% peanut bran and rapeseed meal are added as auxiliary materials to the organic carrier to obtain a raw material mixture. The moisture content of the raw material mixture is adjusted to 50 wt%-60 wt% to obtain a compost pile. After the temperature of the compost pile drops below 35 ℃, the microbial inoculant is introduced into the compost pile at a solid-liquid ratio of 5:

1. The initial concentration of the degrading bacteria is 1.0 × 10⁻⁶. 9 cfu / mL; or Before composting, the microbial inoculant was inoculated into the organic carrier at a solid-liquid ratio of 5:1, and 10% peanut bran and rapeseed meal were added as auxiliary materials to obtain a raw material mixture. The initial concentration of the degrading bacteria was 1.0 × 10⁻⁶. 9 The concentration of cfu / mL was adjusted to 50 wt%-60 wt% after inoculation to obtain a compost pile. After the temperature of the pile dropped below 35 ℃, the microbial agent was again introduced into the pile at a solid-liquid ratio of 5:

1.

7. The method for preparing bio-organic fertilizer from rice straw degrading microbial agents according to claim 6, characterized in that: The secondary fermentation is carried out in an intelligent composting reactor; The pile was turned over every 2 days; ventilation was carried out for 6 minutes every 1 hour at a rate of 30 L / min. The pile body is maintained at a high temperature of 35-45 ℃ for more than 5 days, and after cooling down to below 35 ℃, the moisture content of the pile body is dried to 30 wt%-40 wt%.

8. The bio-organic fertilizer prepared from rice straw degrading microbial agent according to claim 7 and its application method, characterized in that: The concentration of degrading bacteria in the bio-organic fertilizer is 1.0 × 10⁻⁶. 8 cfu / g dry sample; In field trials, the product was applied as a base fertilizer to the soil before rice transplanting. When the rice was harvested, the rice straw was crushed and returned to the field. After the field was flooded for 7 days, the product was applied again and thoroughly mixed with the soil and rice straw.

Images