Full-automatic multifunctional biological online detection device

Abstract

The invention discloses a full-automatic multifunctional biological online detection device, which comprises a sampling module, a sample reserving module, a sample processing module, a detection analysis module and a control module, and is characterized in that the sampling module is used for feeding a sample into the sample processing module; the sample reserving module is used for storing samples; the sample processing module is used for processing a sample, so that the processed sample enters the detection and analysis module; the detection and analysis module is used for analyzing and detecting the processed sample; the control module is used for controlling the sampling module, the sample reserving module, the sample processing module and the detection and analysis module.

Description

Technical Field

[0001] This application relates to the field of online detection technology for biological reaction processes, specifically to a fully automated, multifunctional online biological detection device. Background Technology

[0002] Biological online reaction processes are time-varying, nonlinear, and complex dynamic processes. Although biotechnology has made great strides in genetic engineering and metabolic engineering, enabling the production of high-yielding strains through induced mutations, gene recombination, and culture, optimizing the production of fermentation products remains one of the major challenges in fermentation engineering. Therefore, research on optimization control technologies and intelligent monitoring systems for biological online reaction processes is receiving increasing attention.

[0003] Currently, there are many online detection devices available, primarily targeting physicochemical parameters such as temperature, pH, and dissolved oxygen. These devices can be directly placed in online bioreactors, such as fermenters, to measure these parameters. However, many parameters during the reaction process, such as reducing sugars, organic acids, and synthetic intermediates, cannot be directly detected. This is mainly because the detection devices are not suitable for use in online bioreactors, or the concentration of the analyte is outside the detection range. Although some near-infrared or Raman online detection probes are available on the market that can detect these parameters, they cannot directly output accurate results. Calibration based on offline detection data is still required, which involves long calibration cycles and the calibration curve is easily affected by the fermentation environment. Furthermore, it is currently difficult to find an online detection device that can test all parameters. Therefore, integrating an automatic low-temperature sample retention system is a feature that customers urgently need. Summary of the Invention

[0004] To address the aforementioned technical problems, this application provides a fully automated, multifunctional online biological detection device capable of simultaneously detecting multiple parameters online.

[0005] This application involves the following: This application provides a fully automated, multifunctional online biological detection device, which includes a sampling module, a sample retention module, a sample processing module, a detection and analysis module, and a control module. The sampling module is used to inject samples into the sample processing module; The sample retention module is used to store unprocessed and processed samples from the sample processing module. The sample processing module is used to process the sample; The detection and analysis module is used to analyze and detect both untreated and treated samples. The control module is used to control the sampling module, the sample retention module, the sample processing module, and the detection and analysis module.

[0006] Furthermore, the sampling module includes a sampling component, a sampling driving component, and a feeding component. The sampling component is connected to the feeding component, and the liquid in the feeding component is driven into the sampling component by the sampling driving component.

[0007] Furthermore, the sampling driving component includes a first sampling driving component and a second sampling driving component, wherein the first sampling driving component is used to drive the sample in the sampling component into the sample processing module; The second sampling drive component is used to drive the liquid in the feeding component into the sampling component.

[0008] Furthermore, the device also includes a housing, which includes a first receiving cavity and a second receiving cavity arranged sequentially from top to bottom, and a first fixing plate is disposed between the first receiving cavity and the second receiving cavity.

[0009] Furthermore, the sample retention module includes a metal bath sample retention tray and a cooling component disposed below it. The metal bath sample retention tray includes an outer frame, a metal heat-conducting pipe, and a metal heat-conducting plate. The metal heat-conducting plate is located at the bottom of the outer frame, and the metal heat-conducting pipe is disposed inside the outer frame and connected to the metal heat-conducting plate. The outer frame penetrates the first fixing plate, and the bottom of the outer frame extends into the second receiving cavity.

[0010] Furthermore, the sample retention module also includes a first heat dissipation component, which is located within the first receiving cavity and is positioned toward the metal bath sample retention tray.

[0011] Furthermore, the number of the metal heat pipes is one or more. When the number of the metal heat pipes is two or more, the multiple metal heat pipes are arranged in an array within the outer frame. The metal heat-conducting plate is provided with a slot corresponding to the metal heat-conducting pipe. The side of the metal heat-conducting plate away from the metal heat-conducting pipe is connected to the refrigeration component.

[0012] Furthermore, the sample processing module includes a sample cell, a sample processing chamber, and a first pipetting assembly. The first pipetting assembly is used to transfer the sample in the sample cell to the sample processing cell, and / or The first pipetting assembly is used to transfer the sample, which includes the sample cell and the sample processing cell, to the sample retention module.

[0013] Furthermore, the sample processing module includes a second pipetting assembly, which is used to transfer the sample in the sample processing cell to the detection and analysis module.

[0014] Furthermore, both the sample pool and the sample processing pool are disposed within the second receiving cavity, and both penetrate the first fixing plate, so that their openings are disposed within the first receiving cavity.

[0015] Furthermore, the first pipetting assembly further includes a first pipetting platform and a first pipetting tip, the first pipetting tip being disposed at the bottom of the first pipetting platform, the first pipetting platform driving the first pipetting tip to move, thereby performing pipetting; and / or The second pipetting assembly further includes a second pipetting platform and a second pipetting head. The second pipetting head is provided with the bottom of the second pipetting platform. The second pipetting platform drives the second pipetting head to move, thereby performing pipetting.

[0016] Furthermore, the first pipetting platform includes a first X-axis drive unit, a first Y-axis drive unit, a first Z-axis drive unit, and a first fixing unit, with the first pipetting head disposed at the lower end of the first fixing unit. The first X-axis drive unit drives the first fixing unit and the first pipette head to move back and forth along the first direction; The first Y-axis driving part drives the first fixing part and the first pipette head to move back and forth along the second direction; The first Z-axis drive unit drives the first fixing unit and the first pipette head to move back and forth along a third direction; and / or.

[0017] The second pipetting platform includes a rotary drive unit, a second Y-axis drive unit, and a second fixing unit. The second pipetting head is disposed at the lower end of the second fixing unit. The rotary drive unit drives the second fixed part and the second pipette head to perform circular motion along the rotation axis of the second fixed part. The second Z-axis drive unit drives the second fixing unit and the second pipette head to move back and forth along the third direction.

[0018] Further, the first pipetting assembly includes a first pumping unit, which is connected to the first pipetting head via a first pipetting tube. Preferably, the first pipetting tube passes through the first fixing portion and is connected to the first pipetting head; and / or The second pipetting assembly includes a second pumping unit, which is connected to the second pipetting head via a second pipetting tube. Preferably, the second pipetting tube passes through the second fixing part and is connected to the second pipetting head.

[0019] Furthermore, the first X-axis drive unit includes a first X-axis base plate, a first X-axis guide rail, and a first X-axis drive unit. The first X-axis base plate is parallel to the first fixed plate, and both ends of the first X-axis base plate are respectively connected to the housing. The first X-axis guide rail is mounted on the first X-axis base plate; the first X-axis drive unit is connected to the first X-axis base plate; A first X-axis slider unit is movably disposed on the first X-axis guide rail. The first X-axis drive unit is connected to the first X-axis slider unit, thereby causing the first X-axis drive unit to drive the first X-axis slider unit to move along the first X-axis guide rail.

[0020] Furthermore, the first Y-axis drive unit includes a first Y-axis drive unit, a first Y-axis base plate, and a first Y-axis guide rail. The first Y-axis base plate is connected to the first X-axis slider unit; The first Y-axis guide rail is disposed on the first Y-axis base plate, and the first Y-axis drive unit is connected to the first Y-axis base plate; A first Y-axis slider unit is movably disposed on the first Y-axis guide rail, and the first Y-axis slider unit is connected to the first Z-axis drive unit.

[0021] Furthermore, the first Z-axis drive unit includes a first Z-axis drive unit and a first Z-axis movement unit, both of which are perpendicular to the first fixed plate. The first Z-axis moving unit is connected to the first Z-axis driving unit, and the first Z-axis driving unit drives the first Z-axis moving unit, so that the first Z-axis moving unit moves back and forth relative to the first Z-axis driving unit along a third direction. The first Z-axis moving unit is connected to the first fixed part.

[0022] Furthermore, both the rotary drive unit and the second Y-axis drive unit are disposed within the second receiving cavity, and the second fixing unit passes through the first fixing plate and is connected to the rotary drive unit and the second Y-axis drive unit.

[0023] Furthermore, the second fixing part includes a rotating shaft and a connecting unit. The rotation axis is perpendicular to the first fixed plate. One end of the rotating shaft passes through the first fixed plate and is connected to the rotating drive unit and the second Y-axis drive unit. The other end of the rotating shaft is connected to the connecting unit. The connecting unit is parallel to the first fixing plate. The connecting unit is equipped with the second pipette tip.

[0024] Furthermore, the detection and analysis module includes an enzyme membrane detection component, an optical detection component, and an ion detection component. The enzyme membrane detection component is disposed in the first accommodating cavity, and the optical detection component and the ion detection component are both disposed in the third accommodating cavity; The sample in the sample processing pool is transferred to the enzyme membrane detection component for detection via the second pipetting assembly; The sample cell and sample processing cell are connected to the optical detection component and the ion detection component so that the sample in the sample processing cell can enter the optical detection component and the ion detection component for detection.

[0025] Furthermore, the device also includes a cleaning module and a waste liquid treatment module, both of which are connected to the sampling module, sample processing module, detection and analysis module, and control module.

[0026] Furthermore, the device also includes a display module connected to the control module, which is used to display the operating status of the device.

[0027] The fully automated multifunctional online biological detection device described in this application controls a sampling module, a sample retention module, a sample processing module, and a detection and analysis module through the control module. This allows the sample to be transferred from the sampling module to the sample processing module. The sample retention module stores samples that do not need to be processed temporarily. The sample processing module processes the sample, and the processed sample enters the detection and analysis module for detection, thereby completing the online detection process. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the fully automated multifunctional online biological detection device provided in this application.

[0029] Figure 2 This is a schematic diagram of the fully automated multifunctional online biological detection device provided in this application.

[0030] Figure 3 This is a schematic diagram of the sample retention module provided in this application.

[0031] Figure 4 This is a schematic diagram of the structure of the first pipetting assembly provided in this application.

[0032] Figure 5 This is a partial structural schematic diagram of the first pipetting assembly provided in this application.

[0033] Explanation of reference numerals in the attached figures 1-Housing shell, 2-First receiving cavity, 3-Second receiving cavity, 4-Sample retention module, 5-First X-axis slider unit, 6-Display module, 7-First fixing part, 8-Second pipetting assembly, 9-Sample cell, 10-Sample processing cell A, 11-Sample processing cell B, 12-First X-axis base plate, 13-Optical detection assembly, 14-Enzyme membrane detection assembly, 15-Ion detection assembly, 16-Enzyme membrane electrode, 17-Enzyme membrane reaction cell, 18-Metal heat-conducting pipe, 19 - Outer frame, 20- First pipette head, 21- Metal heat-conducting plate, 22- Cooling assembly, 23- Metal bath sample tray, 24- First X-axis guide rail, 25- First Z-axis drive unit, 26- Gantry frame, 27- First slider A, 28- First slider B, 29- First slider C, 30- X-axis belt, 31- Strip hole, 32- Y-axis belt, 33- First Y-axis base plate, 34- First Y-axis guide rail, 35- First Y-axis slider, 36- Cable chain. Detailed Implementation

[0034] The present application will now be described in detail with reference to the accompanying drawings, wherein the same numerals in all the drawings denote the same features. While specific embodiments of the present application are shown in the drawings, it should be understood that the present application may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this application will be thorough and complete, and will fully convey the scope of the present application to those skilled in the art.

[0035] As used throughout the specification and claims, the terms "comprising" or "including" are open-ended and should be interpreted as "comprising but not limited to". The subsequent descriptions in the specification are preferred embodiments for carrying out this application; however, these descriptions are for the purpose of understanding the general principles of the specification and are not intended to limit the scope of this application. The scope of protection of this application shall be determined by the appended claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains.

[0036] like Figures 1-5 As shown, this application provides a fully automated, multifunctional online biological detection device, which includes a sampling module, a sample retention module 4, a sample processing module, a detection and analysis module, and a control module. The sampling module is used to inject samples into the sample processing module; The sample retention module 4 is used to store unprocessed and processed samples from the sample processing module; The sample processing module is used to process the sample so that the processed sample can enter the detection and analysis module. The detection and analysis module is used to analyze and detect both untreated and treated samples. The control module is used to control the sampling module, the sample retention module 4, the sample processing module, and the detection and analysis module.

[0037] The fully automated multifunctional online biological detection device described in this application controls the sampling module, the sample retention module 4, the sample processing module, and the detection and analysis module through the control module. This allows the sample to be transferred from the sampling module to the sample processing module. The sample retention module 4 stores samples that do not need to be processed temporarily. The sample processing module processes the sample, and the processed sample enters the detection and analysis module for detection, thereby completing the online detection process.

[0038] In this application, the device further includes a cleaning module and a waste liquid treatment module, both of which are connected to the sampling module, sample processing module, detection and analysis module, and control module.

[0039] The cleaning module is used to provide cleaning solution to the sampling module, sample processing module, and detection and analysis module to clean them. This design allows multiple modules to be cleaned simultaneously, resulting in higher efficiency.

[0040] The waste liquid treatment module is used to store the waste liquid discharged from the sampling module, sample processing module and detection and analysis module. This design allows multiple modules to discharge waste simultaneously, resulting in higher efficiency.

[0041] In this application, the sampling module includes a sampling component, a sampling driving component, and a feeding component. The sampling component is connected to the feeding component, and the liquid in the feeding component is driven into the sampling component by the sampling driving component.

[0042] Furthermore, the sampling driving component includes a first sampling driving component and a second sampling driving component. The first sampling driving component is used to drive the sample in the sampling component into the sample processing module; the second sampling driving component is used to drive the liquid in the feeding component into the sampling component.

[0043] The feeding component and the sampling component are connected by a pipeline A, on which a second sampling drive component is provided to control the speed of the feeding process and to start and stop the feeding process.

[0044] The sampling component and the sample processing module are connected by a pipe B, and a first sampling drive component is provided on the pipe B to adjust the sampling speed and to start and stop the sampling process.

[0045] In some implementations, both the first sampling drive component and the second sampling drive component can be peristaltic pumps.

[0046] Furthermore, the sampling drive assembly further includes a third sampling drive assembly and a fourth sampling drive assembly. The sampling assembly is connected to the cleaning module via pipe C, and the third sampling drive assembly is installed on pipe C to facilitate cleaning the sampling assembly. The sampling assembly is connected to the waste liquid treatment module via pipe D, and the fourth sampling drive assembly is installed on pipe D to facilitate discharging waste liquid from the sampling assembly.

[0047] The sampling component can be located inside or outside the housing 1.

[0048] In this application, the device further includes a housing 1, which includes a first receiving cavity 2 and a second receiving cavity 3 arranged sequentially from top to bottom, and a first fixing plate is disposed between the first receiving cavity 2 and the second receiving cavity 3.

[0049] In this application, the sample retention module 4 includes a metal bath sample retention tray 23 and a cooling component 22 disposed below it. The metal bath sample retention tray 23 includes an outer frame 19, a metal heat-conducting pipe 18, and a metal heat-conducting plate 21. The metal heat-conducting plate 21 is located at the bottom of the outer frame 19. The metal heat-conducting pipe 18 is disposed inside the outer frame 19 and is connected to the metal heat-conducting plate 21. The outer frame 19 passes through the first fixing plate, and the bottom of the outer frame 19 extends into the second receiving cavity 3.

[0050] Furthermore, the sample retention module 4 also includes a first heat dissipation component, which is located inside the first receiving cavity 2 and is positioned toward the metal bath sample retention tray 23.

[0051] The first heat dissipation component is disposed on the first fixing plate and is positioned facing and above the opening of the metal heat-conducting pipe 18, so as to disperse the water vapor formed above the metal heat-conducting pipe 18 during the operation of the sample retention module 4. This design, through the first heat dissipation component, can prevent condensation from forming on the top of the sample bottle placed inside the metal heat-conducting pipe, thereby preventing condensation from being introduced into the sample bottle and contaminating the sample during the sample retention process.

[0052] Furthermore, the number of metal heat pipes 18 is one or more. When the number of metal heat pipes 18 is two or more, the multiple metal heat pipes 18 are arranged in an array within the outer frame 19. The metal heat-conducting plate 21 is provided with slots corresponding to the metal heat pipes 18, so as to confine the metal heat pipes 18 within the corresponding slots on the metal heat-conducting plate 21. The side of the metal heat-conducting plate 21 away from the metal heat-conducting pipe 18 is connected to the refrigeration component 22, and the refrigeration component 22 refrigerates and preserves the sample in the metal heat-conducting pipe 18 through the metal heat-conducting plate 21.

[0053] In some embodiments, the number of metal heat pipes 18 is 50, and there are 5 columns and 10 rows of metal heat pipes 18 inside the outer frame 19. The metal heat-conducting plate 21 has 5 columns and 10 rows of slots, and adjacent slots are equally spaced.

[0054] In this application, the sample processing module includes a sample cell 9, a sample processing chamber, a first pipetting assembly, and a second pipetting assembly 8. The first pipetting assembly is used to transfer the sample in the sample cell 9 to the sample processing chamber. Alternatively, the first pipetting assembly can transfer the sample, including the sample cell 9 and the sample processing chamber, to a sample retention module, preferably to a metal heat-conducting pipe of the sample retention module.

[0055] The second pipetting assembly 8 is used to transfer the sample in the sample processing cell to the detection and analysis module.

[0056] Furthermore, both the sample pool 9 and the sample processing pool are disposed within the second receiving cavity 3, and both penetrate the first fixing plate, so that their openings are disposed within the first receiving cavity 2.

[0057] The number of sample processing pools can be more than one, for example, one, two, three, four, five, six, etc.

[0058] Furthermore, the first pipetting assembly also includes a first pipetting platform and a first pipetting head 20. The first pipetting head 20 is disposed at the bottom of the first pipetting platform, and the first pipetting platform drives the first pipetting head 20 to move, thereby performing pipetting.

[0059] The first pipette tip 20 is detachably connected to the first pipetting platform. The first pipette tip 20 can be disposable or non-disposable.

[0060] The first pipette tip 20 can be in 1ml, 5ml, or 10ml sizes.

[0061] Furthermore, the first pipetting platform includes a first X-axis drive unit, a first Y-axis drive unit, a first Z-axis drive unit 25, and a first fixing unit 7, with the first pipetting head 20 disposed at the lower end of the first fixing unit 7. The first X-axis drive unit drives the first fixing part 7 and the first pipette head 20 to move back and forth along the first direction; The first Y-axis driving part drives the first fixing part 7 and the first pipette head 20 to move back and forth along the second direction; The first Z-axis drive unit 25 drives the first fixing unit 7 and the first pipetting head 20 to move back and forth along the third direction.

[0062] Furthermore, the first pipetting assembly includes a first pumping unit, which is connected to the first pipette head 20 via a first pipette tube. Preferably, the first pipette tube passes through the first fixing part 7 and is connected to the first pipette head 20. The first pipette head 20 can aspirate or release samples by rotating the first pumping unit clockwise or counterclockwise. In some embodiments, the first pumping unit can be a peristaltic pump.

[0063] When it is necessary to transfer the sample in the sample cell 9 to the sample processing cell, the first fixing part 7 and the first pipette 20 are moved to the top of the sample cell 9 by the first X-axis drive unit, the first Y-axis drive unit, and the first Z-axis drive unit 25. Then, the first pumping unit is turned on, and the first pipette 20 draws the sample from the sample cell 9. After the sample absorption is completed, the first pumping unit stops running. Then, when the first fixing part 7 and the first pipette 20 are moved above the sample processing cell by the first X-axis drive unit, the first Y-axis drive unit, and the first Z-axis drive unit 25, the first pumping unit is turned on again so that the sample in the first pipette 20 is transferred to the sample processing cell.

[0064] The first X-axis drive unit, the first Y-axis drive unit, the first Z-axis drive unit 25, and the first fixing unit 7 are all located within the first receiving cavity 2. The first pumping unit is located within the second receiving cavity 3.

[0065] Furthermore, the first X-axis drive unit includes a first X-axis base plate 12, a first X-axis guide rail 24, and a first X-axis drive unit. The first X-axis base plate 12 is parallel to the first fixed plate, and both ends of the first X-axis base plate 12 are respectively connected to the housing 1. The first X-axis guide rail 24 is disposed on the first X-axis base plate 12; the first X-axis drive unit is connected to the first X-axis base plate 12.

[0066] A first X-axis slider unit 5 is movably disposed on the first X-axis guide rail 24. The first X-axis drive unit is connected to the first X-axis slider unit 5, thereby causing the first X-axis drive unit to drive the first X-axis slider unit 5 to move along the first X-axis guide rail 24.

[0067] Specifically, the extension direction of the first X-axis guide rail 24 is the same as the extension direction of the first X-axis to the base plate 12, both being the first direction.

[0068] Specifically, a strip-shaped hole 31 is provided in the middle of the first X-axis base plate 12 along the first direction. There are two first X-axis guide rails 24. The two first X-axis guide rails 24 (first X-axis guide rail A and first X-axis guide rail B) are located on both sides of the strip-shaped hole 31, and the two first X-axis guide rails 24 are arranged in parallel.

[0069] Specifically, the first X-axis slider unit 5 includes a gantry 26, on which three sliders are disposed: a first slider A 27, a first slider B 28, and a first slider C 29. The first slider A 27 is movably disposed on the first X-axis guide rail A, and the first slider B 28 is movably disposed on the first X-axis guide rail B. The first slider C 29 is connected to the first X-axis drive unit.

[0070] Specifically, the first X-axis drive unit includes a first X-axis drive motor, an X-axis pulley, and an X-axis belt 30. The first X-axis drive motor is connected to the X-axis pulley, and the X-axis belt 30 is disposed on the X-axis pulley. The first X-axis drive motor drives the X-axis pulley to rotate, thereby driving the X-axis belt 30 to rotate.

[0071] Specifically, the X-axis pulley and X-axis belt 30 are both located inside the strip hole 31. The first X-axis drive motor and the X-axis pulley are both connected to the first X-axis base plate 12. The first slider C 29 is connected to the X-axis belt 30, thereby driving the gantry frame 26 to move along the first direction.

[0072] Furthermore, the first Y-axis drive unit includes a first Y-axis drive unit, a first Y-axis base plate 33, and a first Y-axis guide rail 34. The first Y-axis base plate 33 is connected to the first X-axis slider unit 5. The first Y-axis guide rail 34 is disposed on the first Y-axis base plate 33, and the first Y-axis drive unit is connected to the first Y-axis base plate 33. The first Y-axis slider unit is movably disposed on the first Y-axis guide rail 34, and the first Y-axis slider unit is connected to the first Z-axis drive unit 25.

[0073] Specifically, the first Y-axis base plate 33 is connected to the gantry frame 26. Both the first Y-axis base plate 33 and the first Y-axis guide rail 34 extend in the second direction. The first Y-axis guide rail 34 is disposed on the surface of the first Y-axis base plate 33 away from the first X-axis base plate 12.

[0074] Specifically, the first Y-axis drive unit includes a first Y-axis drive motor, a Y-axis pulley, and a Y-axis belt 32. The first Y-axis drive motor is connected to the Y-axis pulley, and the Y-axis belt 32 is disposed on the Y-axis pulley. The first Y-axis drive motor drives the Y-axis pulley to rotate, thereby driving the Y-axis belt 32 to rotate.

[0075] Specifically, the first Y-axis drive motor and the Y-axis pulley are both connected to the first Y-axis base plate 33, and the Y-axis pulley and the Y-axis belt 32 are located on one side of the first Y-axis base plate 33. The Y-axis belt 32 is connected to the first Y-axis slider unit, thereby driving the first Y-axis slider unit to move along the second direction on the first Y-axis guide rail 34.

[0076] In some embodiments, the first Y-axis slider unit includes a first Y-axis slider 35 and a cable chain 36. The first Y-axis slider 35 is connected to both the cable chain 36 and the first Z-axis drive unit 25. The main function of the cable chain 36 is to unify the routing of the wiring within the first Y-axis drive unit, improving aesthetics and preventing the wiring from being pulled during the movement of the motion mechanism.

[0077] Furthermore, the first Z-axis drive unit 25 includes a first Z-axis drive unit and a first Z-axis moving unit. Both the first Z-axis drive unit and the first Z-axis moving unit are perpendicular to the first fixed plate. The first Z-axis moving unit is connected to the first Z-axis drive unit, and the first Z-axis drive unit drives the first Z-axis moving unit, causing the first Z-axis moving unit to move back and forth relative to the first Z-axis drive unit along a third direction. The first Z-axis moving unit is connected to the first fixed part 7.

[0078] Furthermore, the second pipetting assembly 8 also includes a second pipetting platform and a second pipetting head. The second pipetting head is provided with the bottom of the second pipetting platform, and the second pipetting platform drives the second pipetting head to move, thereby performing pipetting.

[0079] The second pipette tip is detachably connected to the second pipetting platform. The second pipette tip can be disposable or non-disposable.

[0080] The second pipette tip can be in 1ml, 5ml, or 10ml sizes.

[0081] Furthermore, the second pipetting assembly 8 includes a second pumping unit, which is connected to the second pipette tip via a second pipette tube. Preferably, the second pipette tube passes through the second fixing portion and is connected to the second pipette tip. The second pipette tip can aspirate or release samples by rotating the second pumping unit clockwise or counterclockwise. In some embodiments, the second pumping unit can be a peristaltic pump.

[0082] Furthermore, the second pipetting platform includes a rotary drive unit, a second Y-axis drive unit, and a second fixed unit. The lower end of the second fixed unit is provided with the second pipetting head. The rotary drive unit drives the second fixed unit and the second pipetting head to perform circumferential motion along the rotation axis of the second fixed unit. The second Z-axis drive unit drives the second fixed unit and the second pipetting head to move back and forth along the third direction.

[0083] Specifically, the openings of the sample cell 9 and the sample processing cell are positioned on the rotation trajectory of the second pipetting platform to facilitate sampling.

[0084] When it is necessary to transfer the sample in the sample processing cell to the detection and analysis module (enzyme membrane detection component 14), the rotary drive unit drives the second fixing unit and the second pipette head to move in a circular motion along the rotation axis of the second fixing unit. Since the openings of the sample cell 9 and the sample processing cell are set on the rotation trajectory of the second pipetting platform, when the second pipette head moves to directly above the opening of the sample cell 9 or the sample processing cell, the operation stops, the second Y-axis drive unit is turned on, and the second Z-axis drive unit drives the second pipette head to extend into the sample cell 9 or the sample processing cell to aspirate the sample. After the sample is aspirated, the second pipette head is driven to rise again to leave the opening of the sample cell 9 or the sample processing cell. Then the rotary drive unit is turned on so that it drives the second pipette head to transfer to the detection and analysis module (enzyme membrane detection component 14) for detection.

[0085] Specifically, the rotary drive unit and the second Y-axis drive unit are both disposed in the second receiving cavity 3, and the second fixing unit passes through the first fixing plate and is connected to the rotary drive unit and the second Y-axis drive unit.

[0086] Both the rotary drive unit and the second Y-axis drive unit are conventional drive devices, and their specific structures are not further limited.

[0087] Specifically, the second fixing part includes a rotating shaft and a connecting unit. The rotating shaft is perpendicular to the first fixing plate. One end of the rotating shaft passes through the first fixing plate and is connected to the rotating drive part and the second Y-axis drive part. The other end of the rotating shaft is connected to the connecting unit. The connecting unit is parallel to the first fixing plate. The connecting unit is provided with a second pipette. The second pipette is perpendicular to the connecting unit and parallel to the rotating shaft.

[0088] In this application, the detection and analysis module includes an enzyme membrane detection component 14, an optical detection component 13, and an ion detection component 15. The enzyme membrane detection component 14 is disposed in the first accommodating cavity 2, and the optical detection component 13 and the ion detection component 15 are both disposed in the third accommodating cavity.

[0089] Furthermore, the samples in the sample pool 9 and the sample processing pool are transferred to the enzyme membrane detection component 14 for detection via the second pipetting component 8.

[0090] Furthermore, the enzyme membrane detection assembly 14 includes an enzyme membrane reaction chamber 17 and an enzyme membrane electrode 16 and an enzyme membrane disposed in the enzyme membrane reaction chamber 17.

[0091] The enzyme membrane reaction chamber 17 is connected to a buffer bottle containing buffer solution via a pipe E. An enzyme membrane driving component A is provided on the pipe E to facilitate the injection of buffer solution into the enzyme membrane reaction chamber 17.

[0092] The enzyme membrane reaction tank 17 is connected to the waste liquid treatment module through pipeline F. An enzyme membrane driving component B is installed on the pipeline F to facilitate the discharge of waste liquid from the enzyme membrane reaction tank 17.

[0093] The enzyme membrane driving component A and enzyme membrane driving component B can be peristaltic pumps.

[0094] Specifically, the enzyme membrane detection component 14 further includes a standard solution A storage tube and a standard solution B storage tube. Both the standard solution A storage tube and the standard solution B storage tube are disposed in the second receiving cavity 3, and both the standard solution A storage tube and the standard solution B storage tube penetrate the first fixing plate. The tube openings are both located in the first receiving cavity 2, and the tube openings of both the standard solution A storage tube and the standard solution B storage tube are both located on the rotation trajectory of the second pipetting platform.

[0095] When enzyme membrane testing is required, standard solutions A and B, and the liquid in sample cell 9 or sample processing cell are pipetted into the enzyme membrane reaction cell 17 via the second pipetting assembly 8. In non-working mode, the enzyme membrane reaction cell 17 is filled with buffer solution. During operation, the buffer solution is drained through tubing F, and then injected into the enzyme membrane reaction cell 17 via the enzyme membrane drive assembly A. Then, standard solution A or B is transferred to the enzyme membrane reaction cell 17 via the second pipetting assembly 8 for enzyme membrane detection and calibration. After detection, the liquid in the enzyme membrane reaction cell 17 is drained through tubing F, and then the liquid sample from sample cell 9 or sample processing cell is added to the enzyme membrane reaction cell 17 using the same method as the standard solution for detection. After detection, the reaction cell is cleaned with buffer solution from the buffer bottle 3-4 times, and then refilled with buffer solution for the next sample test.

[0096] The enzyme membrane can be one or more of the following: glucose enzyme membrane, glycerol enzyme membrane, lactase membrane, glutamate membrane, glutaminase membrane, lysine enzyme membrane, alcohol enzyme membrane, methanol enzyme membrane, lactase membrane, galactase membrane, xylase membrane, and sucrase membrane assembly.

[0097] Furthermore, the number of enzyme membrane detection components 14 can be one or more, and the number of enzyme membrane detection components 14 can be determined according to actual needs. To ensure the enzyme membrane reaction is carried out at a constant temperature, a temperature control device is provided below the enzyme membrane reaction tank 17.

[0098] Furthermore, the sample processing cell is connected to the optical detection component 13 and the ion detection component 15 so that the sample in the sample processing cell can enter the optical detection component 13 and the ion detection component 15 for detection.

[0099] Both the optical detection component 13 and the ion detection component 15 are located within the second receiving cavity 3. The optical detection component 13 is mounted on the first fixing plate, and a first window is provided on the first fixing plate, corresponding to the optical detection component 13. A first cover plate is also provided on the first window, which can cover the first window. The purpose of designing the first window is to facilitate the user to periodically remove the cuvette from the optical detection component for cleaning and maintenance.

[0100] The optical detection component 13 is connected to the cleaning module via a pipeline G. An optical detection drive component A is provided on the pipeline G to facilitate the injection of cleaning fluid from the cleaning module into the optical detection module.

[0101] The optical detection component 13 is connected to the waste liquid treatment module through the pipeline H. The pipeline H is equipped with an optical detection drive component B to facilitate the discharge of waste liquid from the optical detection component 13.

[0102] The optical detection component 13 is connected to the sample processing cell via a pipeline I. An optical detection drive component C is provided on the pipeline I to facilitate the transfer of the sample in the sample processing cell to the optical detection component 13.

[0103] The optical detection drive component A, optical detection drive component B, and optical detection drive component C can be peristaltic pumps.

[0104] The optical detection component 13 can be a spectrophotometer.

[0105] A second window is provided in the area of ​​the housing 1 corresponding to the second receiving cavity 3. The second window corresponds to the ion detection component 15. A second cover plate is also provided on the second window and can cover the second window. The purpose of designing the second window is to facilitate the user's regular maintenance of the components in the second receiving cavity 3.

[0106] The ion detection component 15 is connected to the cleaning module through the pipeline J. The pipeline J is equipped with an ion detection driving component A to facilitate the injection of cleaning fluid from the cleaning module into the ion detection module.

[0107] The ion detection component 15 is connected to the waste liquid treatment module through pipeline K. An ion detection driving component B is provided on the pipeline K to facilitate the discharge of waste liquid from the ion detection component 15.

[0108] The ion detection component 15 is connected to the sample processing cell via a pipeline L. An ion detection driving component C is provided on the pipeline L to facilitate the transfer of the sample in the sample processing cell to the ion detection component 15.

[0109] The ion detection driving component A, ion detection driving component B, and ion detection driving component C can be peristaltic pumps.

[0110] The ion detection component 15 can be one or more of the following: pH electrode component, ammonia ion electrode component, sodium ion electrode component, potassium ion electrode component, calcium ion electrode component, magnesium ion electrode component, and nitrate ion electrode component, used to detect the concentration of relevant ions in the sample.

[0111] In this application, the device further includes a display module 6, which is connected to the control module and is used to display the working status of the device.

[0112] The display module 6 is disposed outside the housing 1 and on the top of the housing 1 to facilitate observation of the operation of each module.

[0113] The control module can be located in the first receiving cavity 2 or the second receiving cavity 3, so as to control each module and control the operation of the device.

[0114] Example This application provides a general and / or specific description of the materials and test methods used in the experiments. In the following examples, unless otherwise specified, % represents wt%, i.e., weight percentage. Reagents or instruments used, unless otherwise specified, are all commercially available conventional reagent products.

[0115] Example 1 like Figure 1-5 As shown, the fully automated multifunctional online biological detection device of this embodiment includes a housing 1, a sampling module, a sample retention module 4, a sample processing module, a detection and analysis module, a control module, a display module 6, a cleaning module, and a waste liquid treatment module. The control module is connected to the sampling module, sample retention module 4, sample processing module, detection and analysis module, display module 6, cleaning module, and waste liquid treatment module respectively, thereby controlling the sampling module, sample retention module 4, sample processing module, detection and analysis module, cleaning module, and waste liquid treatment module, and displaying their working status on the display module 6.

[0116] The housing 1 has a horizontally arranged first fixing plate, which divides the cavity inside the housing 1 into a first receiving cavity 2 and a second receiving cavity 3, with the first receiving cavity 2 located above the second receiving cavity 3.

[0117] The sampling module includes a sampling component, a sampling drive component, a feeding component, a first sampling drive component, a second sampling drive component, a third sampling drive component, and a fourth sampling drive component. The feeding component is connected to the sampling component via pipe A, and the second sampling drive component is mounted on pipe A. The sampling component is connected to the sample processing module via pipe B, and the first sampling drive component is mounted on pipe B. The sampling component is connected to the cleaning module via pipe C, and the third sampling drive component is mounted on pipe C to facilitate cleaning the sampling component. The sampling component is connected to the waste liquid treatment module via pipe D, and the fourth sampling drive component is mounted on pipe D to facilitate the discharge of waste liquid from the sampling component. The first, second, third, and fourth sampling drive components are all peristaltic pumps.

[0118] The sample retention module 4 includes a metal bath sample retention tray 23, a cooling component 22 disposed below it, and a first heat dissipation component. The metal bath sample retention tray 23 includes an outer frame 19, a metal heat-conducting pipe 18, and a metal heat-conducting plate 21. The metal heat-conducting plate 21 is located at the bottom of the outer frame 19. The metal heat-conducting pipe 18 is disposed inside the outer frame 19 and connected to the metal heat-conducting plate 21. The metal heat-conducting plate 21 is provided with a slot corresponding to the metal heat-conducting pipe 18 to confine the metal heat-conducting pipe 18 within the corresponding slot. The outer frame 19 penetrates the first fixing plate, and the bottom of the outer frame 19 extends into the second receiving cavity 3. The first heat dissipation component is located inside the first receiving cavity 2 and is disposed on the first fixing plate, facing the opening of the metal heat-conducting pipe 18 and above the opening, so as to disperse the water vapor formed above the metal heat-conducting pipe 18 during the operation of the sample retention module 4. The number of metal heat pipes 18 is 50, and there are 5 columns and 10 rows of metal heat pipes 18 inside the outer frame 19. The metal heat-conducting plate 21 has 5 columns and 10 rows of slots, and adjacent slots are equally spaced.

[0119] The sample processing module includes a sample cell 9, a sample processing cell A 10, a sample processing cell B 11, a first pipetting assembly, and a second pipetting assembly 8. The first pipetting assembly is used to transfer the sample in the sample cell 9 to the sample processing cell A 10 and the sample processing cell B 11, and the second pipetting assembly 8 is used to transfer the sample in the sample processing cell to the detection and analysis module.

[0120] The first pipetting assembly further includes a first pipetting platform, a first pipetting head 20, and a first pumping unit. The first pipetting platform includes a first X-axis drive unit, a first Y-axis drive unit, a first Z-axis drive unit 25, and a first fixing part 7. The first pipetting head 20 is detachably mounted on the lower end of the first fixing part 7. The first pumping unit is connected to the first pipetting head 20 via a first pipetting tube, which passes through the first fixing part 7 and is connected to the first pipetting head 20.

[0121] The first X-axis drive unit includes a first X-axis base plate 12, a first X-axis guide rail 24, and a first X-axis drive unit. The first X-axis base plate 12 is parallel to the first fixed plate, and both ends of the first X-axis base plate 12 are connected to the housing 1. The first X-axis guide rail 24 is disposed on the first X-axis base plate 12. The first X-axis drive unit is connected to the first X-axis base plate 12. A first X-axis slider unit 5 is movably disposed on the first X-axis guide rail 24, and the first X-axis drive unit is connected to the first X-axis slider unit 5. The extension direction of the first X-axis guide rail 24 is the same as the extension direction of the first X-axis base plate 12, both being the first direction. A strip-shaped hole 31 is formed in the middle of the first X-axis base plate 12 along the first direction. There are two first X-axis guide rails 24, namely first X-axis guide rail A and first X-axis guide rail B, which are located on both sides of the strip-shaped hole 31, and the two first X-axis guide rails 24 are arranged in parallel. The first X-axis slider unit 5 includes a gantry frame 26, on which three sliders are mounted: a first slider A 27, a first slider B 28, and a first slider C 29. The first slider A 27 is movably mounted on the first X-axis guide rail 24A, and the first slider B 28 is movably mounted on the first X-axis guide rail B. The first slider C 29 is connected to the first X-axis drive unit. The first X-axis drive unit includes a first X-axis drive motor, an X-axis pulley, and an X-axis belt 30. The first X-axis drive motor is connected to the X-axis pulley, and the X-axis belt 30 is mounted on the X-axis pulley. The first X-axis drive motor drives the X-axis pulley to rotate, thereby driving the X-axis belt 30 to rotate. The X-axis pulley and X-axis belt 30 are both located in the area within the strip hole 31. The first X-axis drive motor and the X-axis pulley are both connected to the first X-axis base plate 12. The first slider C 29 is connected to the X-axis belt 30, thereby driving the gantry frame 26 to move along the first direction.

[0122] The first Y-axis drive unit includes a first Y-axis drive unit, a first Y-axis base plate 33, and a first Y-axis guide rail 34. The first Y-axis base plate 33 is connected to the gantry frame 26. The first Y-axis guide rail 34 is disposed on the surface of the first Y-axis base plate 33 away from the first X-axis base plate 12. The extension directions of both the first Y-axis base plate 33 and the first Y-axis guide rail 34 are in the second direction. The first Y-axis drive unit is connected to the first Y-axis base plate 33. A first Y-axis slider unit is movably disposed on the first Y-axis guide rail 34, and the first Y-axis slider unit is connected to the first Z-axis drive unit 25. The first Y-axis drive unit includes a first Y-axis drive motor, a Y-axis pulley, and a Y-axis belt 32. The first Y-axis drive motor is connected to the Y-axis pulley, and the Y-axis belt 32 is disposed on the Y-axis pulley. The first Y-axis drive motor drives the Y-axis pulley to rotate, thereby driving the Y-axis belt 32 to rotate. The first Y-axis drive motor and the Y-axis pulley are both connected to the first Y-axis base plate 33, and the Y-axis pulley and the Y-axis belt 32 are located on one side of the first Y-axis base plate 33. The Y-axis belt 32 is connected to the first Y-axis slider unit, thereby driving the first Y-axis slider unit to move along the second direction on the first Y-axis guide rail 34. The first Y-axis slider unit includes a first Y-axis slider 35 and a cable chain 36, and the first Y-axis slider 35 is connected to the cable chain 36 and the first Z-axis drive unit 25 respectively.

[0123] The first Z-axis drive unit 25 includes a first Z-axis drive unit and a first Z-axis moving unit. Both the first Z-axis drive unit and the first Z-axis moving unit are perpendicular to the first fixed plate. The first Z-axis moving unit is connected to the first Z-axis drive unit and the first Z-axis moving unit is connected to the first fixed part 7.

[0124] The second pipetting assembly 8 further includes a second pipetting platform, a second pipetting head, and a second pumping unit. The second pumping unit is connected to the second pipetting head via a second pipetting tube, which passes through the second fixing part and connects to the second pipetting head. The second pipetting platform includes a rotary drive, a second Y-axis drive, and a second fixing part. The second pipetting head is detachably mounted on the lower end of the second fixing part. The rotary drive drives the second fixing part and the second pipetting head to move in a circular motion along the rotation axis of the second fixing part; the second Z-axis drive drives the second fixing part and the second pipetting head to move back and forth in a third direction. The openings of the sample cell 9 and the sample processing cell are located on the rotation trajectory of the second pipetting platform. The rotary drive and the second Y-axis drive are both located within the second receiving cavity 3, and the second fixing part passes through the first fixing plate and connects to the rotary drive and the second Y-axis drive. The second fixing part includes a rotating shaft and a connecting unit. The rotating shaft is perpendicular to the first fixing plate. One end of the rotating shaft passes through the first fixing plate and is connected to the rotating drive part and the second Y-axis drive part. The other end of the rotating shaft is connected to the connecting unit. The connecting unit is parallel to the first fixing plate. A second pipette is disposed on the connecting unit. The second pipette is perpendicular to the connecting unit and parallel to the rotating shaft. The second pumping unit is a peristaltic pump. Both the first pipette 20 and the second pipette have a capacity of 1 ml.

[0125] The detection and analysis module includes an enzyme membrane detection component 14, an optical detection component 13, and an ion detection component 15. The enzyme membrane detection component 14 is disposed in the first accommodating cavity 2, and the optical detection component 13 and the ion detection component 15 are both disposed in the third accommodating cavity.

[0126] The enzyme membrane detection assembly 14 includes a standard solution A storage tube, a standard solution B storage tube, an enzyme membrane reaction chamber 17, and enzyme membrane electrodes 16 and an enzyme membrane disposed in the enzyme membrane reaction chamber 17. The number of enzyme membrane electrodes 16 can be four. Both the standard solution A storage tube and the standard solution B storage tube are disposed within the second receiving cavity 3, and both penetrate the first fixing plate, with their openings located in the first receiving cavity 2. The openings of both the standard solution A storage tube and the standard solution B storage tube are located on the rotation trajectory of the second pipetting platform. The enzyme membrane reaction chamber 17 is connected to a buffer bottle containing buffer solution via pipe E. An enzyme membrane driving assembly A is disposed on pipe E to facilitate the injection of buffer solution into the enzyme membrane reaction chamber 17. The enzyme membrane reaction chamber 17 is connected to the waste liquid treatment module via pipe F. An enzyme membrane driving assembly B is disposed on pipe F to facilitate the discharge of waste liquid from the enzyme membrane reaction chamber 17. Both the enzyme membrane driving assembly A and the enzyme membrane driving assembly B can be peristaltic pumps.

[0127] Both the optical detection component 13 and the ion detection component 15 are located within the second receiving cavity 3. The optical detection component 13 is mounted on the first fixing plate, and a first window is provided on the first fixing plate, corresponding to the optical detection component 13. A first cover plate is also provided on the first window, which can cover the first window. A second window is provided in the area of ​​the housing 1 corresponding to the second receiving cavity 3, and the second window corresponds to the ion detection component 15. A second cover plate is also provided on the second window, which can cover the second window.

[0128] The optical detection component 13 is connected to the cleaning module via pipe G, and an optical detection driving component A is installed on pipe G. The optical detection component 13 is connected to the waste liquid treatment module via pipe H, and an optical detection driving component B is installed on pipe H. The optical detection component 13 is connected to the sample processing cell A 10 via pipe I, and an optical detection driving component C is installed on pipe I.

[0129] The ion detection component 15 is connected to the cleaning module via pipe J, and an ion detection driving component A is installed on pipe J. The ion detection component 15 is connected to the waste liquid treatment module via pipe K, and an ion detection driving component B is installed on pipe K. The ion detection component 15 is connected to the sample processing cell B 11 via pipe L, and an ion detection driving component C is installed on pipe L. All three components—optical detection driving component A, optical detection driving component B, optical detection driving component C, and ion detection driving component C—are peristaltic pumps.

[0130] The fully automated multifunctional online biological detection device described in this application controls a sampling module, a sample retention module, a sample processing module, and a detection and analysis module through the control module. This allows the sample to be transferred from the sampling module to the sample processing module. The sample retention module stores samples that do not need to be processed temporarily. The sample processing module processes the samples, and the processed samples enter the detection and analysis module for detection, thereby completing the fully automated online detection process, improving detection efficiency, and integrating multiple modules into one device, which greatly saves experimental space.

[0131] The description in this disclosure is provided for illustrative and descriptive purposes only and is not intended to be exhaustive or to limit the disclosure to its forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical application of this disclosure and to enable those skilled in the art to understand this disclosure and to design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A fully automated, multifunctional online biological detection device, wherein, It includes a sampling module, a sample retention module, a sample processing module, a detection and analysis module, and a control module. The sampling module is used to inject samples into the sample processing module; The sample retention module is used to store unprocessed and processed samples from the sample processing module. The sample processing module is used to process the sample; The detection and analysis module is used to analyze and detect both untreated and treated samples. The control module is used to control the sampling module, the sample retention module, the sample processing module, and the detection and analysis module.

2. The apparatus according to claim 1, wherein, The sampling module includes a sampling component, a sampling driving component, and a feeding component. The sampling component is connected to the feeding component, and the liquid in the feeding component is driven into the sampling component by the sampling driving component.

3. The apparatus according to claim 2, wherein, The sampling drive component includes a first sampling drive component and a second sampling drive component, wherein the first sampling drive component is used to drive the sample in the sampling component into the sample processing module; The second sampling drive component is used to drive the liquid in the feeding component into the sampling component.

4. The apparatus according to claim 1, wherein, The device also includes a housing, which includes a first receiving cavity and a second receiving cavity arranged sequentially from top to bottom, and a first fixing plate is disposed between the first receiving cavity and the second receiving cavity.

5. The apparatus according to claim 4, wherein, The sample retention module includes a metal bath sample retention tray and a cooling component disposed below it. The metal bath sample retention tray includes an outer frame, a metal heat-conducting pipe and a metal heat-conducting plate. The metal heat-conducting plate is located at the bottom of the outer frame, and the metal heat-conducting pipe is disposed inside the outer frame and connected to the metal heat-conducting plate. The outer frame penetrates the first fixing plate, and the bottom of the outer frame extends into the second receiving cavity.

6. The apparatus according to claim 5, wherein, The sample retention module further includes a first heat dissipation component, which is located inside the first receiving cavity and is positioned toward the metal bath sample retention tray.

7. The apparatus according to claim 5, wherein, The number of metal heat pipes is one or more. When the number of metal heat pipes is two or more, the multiple metal heat pipes are arranged in an array within the outer frame. The metal heat-conducting plate is provided with a slot corresponding to the metal heat-conducting pipe. The side of the metal heat-conducting plate away from the metal heat-conducting pipe is connected to the refrigeration component.

8. The apparatus according to claim 4, wherein, The sample processing module includes a sample cell, a sample processing chamber, and a first pipetting assembly. The first pipetting assembly is used to transfer the sample in the sample cell to the sample processing cell, and / or The first pipetting assembly is used to transfer the sample, which includes the sample cell and the sample processing cell, to the sample retention module.

9. The apparatus according to claim 4, wherein, The sample processing module includes a second pipetting assembly, which is used to transfer the sample in the sample processing cell to the detection and analysis module.

10. The apparatus according to claim 9, wherein, Both the sample pool and the sample processing pool are located within the second receiving cavity, and both penetrate the first fixing plate, with their openings located within the first receiving cavity.

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