Polypeptide feed

Abstract

The polypeptide feed is prepared from the following raw materials in percentage by mass: 35%-40% of a plant crude extract, 35%-45% of a protein source, 5%-8% of microbial flora and 15%-20% of a fermentation auxiliary material, wherein the plant extract is prepared from the following raw materials in percentage by mass: 50% of an astragalus membranaceus crude extract, 30% of a codonopsis pilosula crude extract and 20% of an angelica sinensis crude extract. The feed contains various plant and animal-derived amino acids, can supplement various nutrients in animal bodies, maintain amino acid balance, and is beneficial to enhancing protein fat absorption, promoting muscle and fat growth and improving meat quality and flavor, and meanwhile, the rich peptide contains various beneficial bacteria and free fatty acids, so that vasoactive intestinal peptide causes vasodilatation of mesentery, and the meat quality and flavor are improved. Protease and digestive enzymes are secreted in quantity, intestinal tract movement is enhanced, digestive absorption and anabolism of the body are enhanced, and therefore growth of the body is promoted.

Description

Technical Field

[0001] This invention relates to the field of animal feed technology, specifically to a polypeptide feed. Background Technology

[0002] Numerous food safety incidents in recent years, such as avian influenza, foot-and-mouth disease, turbot, hemolytic Escherichia coli, and Staphylococcus aureus in dumplings, serve as stark reminders that addressing food safety issues is urgent. The overuse of preventative and therapeutic drugs in livestock farming is one of the main factors contributing to human food safety problems.

[0003] Traditional feed additives mostly rely on chemical synthesis or single plant extraction, which have problems such as unbalanced nutrition, low absorption rate and potential residue risks. Although existing microbial fermentation feed technology can improve protein utilization, it still has shortcomings in terms of strain combination optimization and synergistic effect of functional components. In particular, it has limited preventive effect on viral diseases such as African swine fever, and the uneven distribution of polypeptide molecular weight in fermentation products affects absorption efficiency. Summary of the Invention

[0004] The purpose of this invention is to provide a polypeptide feed to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a polypeptide feed, wherein the polypeptide feed is composed of the following raw materials by mass percentage: 35%-40% crude plant extract, 35%-45% protein source, 5%-8% microbial flora, and 15%-20% fermentation auxiliary material.

[0006] Preferably, the plant extract is composed of the following raw materials in the following proportions by mass percentage: 50% crude extract of Astragalus membranaceus, 30% crude extract of Codonopsis pilosula, and 20% crude extract of Angelica sinensis.

[0007] Preferably, the protein source is composed of the following raw materials in the following proportions by mass percentage: 70% soy protein isolate and 30% fish meal.

[0008] Preferably, the microbial community is composed of the following raw materials in the following proportions by mass percentage: Bacillus subtilis 40%, Bacillus licheniformis 20%, Saccharomyces cerevisiae 20%, and Lactobacillus plantarum 20%.

[0009] Preferably, the fermentation adjuvant is composed of the following raw materials in the following proportions by mass percentage: 90% brown sugar powder, 9% protein powder, 0.5% dipotassium hydrogen phosphate, 0.3% magnesium sulfate, and 0.2% compound vitamins.

[0010] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention contains a variety of plant and animal-derived amino acids, which can supplement various nutrients in the animal body, maintain amino acid balance, promote protein and fat absorption, promote muscle and fat growth, and improve meat flavor. At the same time, it is rich in peptides containing a variety of beneficial bacteria and free fatty acids, which cause vasoactive intestinal peptides to cause vasodilation of the mesentery, stimulate intestinal fluid and gastrointestinal peristalsis, and promote the secretion of large amounts of proteases and digestive enzymes, thereby enhancing intestinal motility, enhancing the body's digestion, absorption, and anabolism, and thus promoting the body's growth.

[0011] 2. This invention can effectively solve the problem of erythropoietin in piglets, giving their skin a rosy and shiny appearance, significantly increasing serum immunoglobulin levels, improving the immune function and growth and development of piglets, promoting blood circulation, preventing anemia, increasing the activity of probiotics in the oral and nasal mucosa, and inhibiting the risk of African swine fever virus infection.

[0012] 3. The feed of the present invention is green, safe, and residue-free, containing no chemical or prohibited ingredients. It can reduce the burden on animals, improve their health, and has good effects. Detailed Implementation

[0013] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0014] Example 1: This invention provides a technical solution: a polypeptide feed, which is composed of the following raw materials by mass percentage: 35% crude plant extract, 40% protein source, 5% microbial flora, and 20% fermentation auxiliary material.

[0015] The plant extract is composed of the following raw materials in the following proportions by mass percentage: 50% crude extract of Astragalus membranaceus, 30% crude extract of Codonopsis pilosula, and 20% crude extract of Angelica sinensis.

[0016] The protein source is composed of the following ingredients in the following proportions by weight: 70% soy protein isolate and 30% fish meal.

[0017] The microbial community is composed of the following raw materials in the following proportions by mass percentage: Bacillus subtilis 40%, Bacillus licheniformis 20%, Saccharomyces cerevisiae 20%, and Lactobacillus plantarum 20%.

[0018] The fermentation adjuvant is composed of the following raw materials in the following proportions by mass percentage: 90% brown sugar powder, 9% protein powder, 0.5% dipotassium hydrogen phosphate, 0.3% magnesium sulfate, and 0.2% compound vitamins.

[0019] The specific production method of the polypeptide feed of the present invention is as follows: S1. Raw material pretreatment: Mix soybean meal and fish meal in proportion, grind to 80 mesh, sterilize at 121℃ for 20 minutes, mix plant crude extract in proportion, and mix with protein source at a mass ratio of 1:2, and adjust the moisture content to 50%. S2. Inoculation and Fermentation of Compound Strains: Bacillus subtilis was cultured on LB medium at 37°C for 12 hours, Bacillus licheniformis on TSB medium at 37°C for 20 hours, Saccharomyces cerevisiae on YPD medium at 28°C for 18 hours, and Lactobacillus plantarum on MRS medium at 37°C for 24 hours. The activated bacterial solutions were then mixed in a specific ratio (Bacillus subtilis: Bacillus licheniformis: Saccharomyces cerevisiae: Lactobacillus plantarum = 4:2:2:2), with a total inoculation amount of 5%. Fermentation was then carried out under the following conditions: temperature 30±1°C, pH 6.8, aeration rate 1.5 L / min, and fermentation time 48 hours. During fermentation, the pH was monitored every 12 hours, and glucose (0.5%) was added to maintain the pH at 6.5-7.0. Free amino acids were added simultaneously with the protein source during fermentation to convert the protein into tetrapeptides. S3. Post-processing and finished product preparation: After fermentation, the cells are inactivated by heat treatment at 80℃ for 10 minutes, followed by spray drying (controlling the inlet air temperature to 180℃ and the outlet air temperature to 80℃) or low-temperature drying (45℃ to moisture content ≤8%).

[0020] The feed produced using the above-mentioned ingredient ratios had the following fermentation product analysis results: polypeptide content of 19.2%, live lactic acid bacteria count of 2.3 × 10^8 CFU / g, and protease activity of 620 U / g.

[0021] Feeding trials on piglets showed that the average daily weight gain was 1.92 kg, the diarrhea rate decreased by 68%, and the feed utilization rate increased by 13%.

[0022] Example 2: This invention provides a technical solution: a polypeptide feed, which is composed of the following raw materials by mass percentage: 45% crude plant extract, 30% protein source, 8% microbial flora, and 17% fermentation auxiliary material.

[0023] The plant extract is composed of the following raw materials in the following proportions by mass percentage: 50% crude extract of Astragalus membranaceus, 30% crude extract of Codonopsis pilosula, and 20% crude extract of Angelica sinensis.

[0024] The protein source is composed of the following ingredients in the following proportions by weight: 70% soy protein isolate and 30% fish meal.

[0025] The microbial community is composed of the following raw materials in the following proportions by mass percentage: Bacillus subtilis 40%, Bacillus licheniformis 20%, Saccharomyces cerevisiae 20%, and Lactobacillus plantarum 20%.

[0026] The fermentation adjuvant is composed of the following raw materials in the following proportions by mass percentage: 90% brown sugar powder, 9% protein powder, 0.5% dipotassium hydrogen phosphate, 0.3% magnesium sulfate, and 0.2% compound vitamins.

[0027] The specific production method of the polypeptide feed of the present invention is as follows: S1. Raw material pretreatment: Mix soybean meal and fish meal in proportion, grind to 80 mesh, sterilize at 121℃ for 20 minutes, mix plant crude extract in proportion, and mix with protein source at a mass ratio of 1:2, and adjust the moisture content to 50%. S2. Inoculation and Fermentation of Compound Strains: Bacillus subtilis was cultured on LB medium at 37°C for 12 hours, Bacillus licheniformis on TSB medium at 37°C for 20 hours, Saccharomyces cerevisiae on YPD medium at 28°C for 18 hours, and Lactobacillus plantarum on MRS medium at 37°C for 24 hours. The activated bacterial solutions were then mixed in a specific ratio (Bacillus subtilis: Bacillus licheniformis: Saccharomyces cerevisiae: Lactobacillus plantarum = 4:2:2:2), with a total inoculation amount of 5%. Fermentation was then carried out under the following conditions: temperature 30±1°C, pH 6.8, aeration rate 1.5 L / min, and fermentation time 48 hours. During fermentation, the pH was monitored every 12 hours, and glucose (0.5%) was added to maintain the pH at 6.5-7.0. Free amino acids were added simultaneously with the protein source during fermentation to convert the protein into tetrapeptides. S3. Post-processing and finished product preparation: After fermentation, the cells are inactivated by heat treatment at 80℃ for 10 minutes, followed by spray drying (controlling the inlet air temperature to 180℃ and the outlet air temperature to 80℃) or low-temperature drying (45℃ to moisture content ≤8%).

[0028] The feed produced using the above-mentioned ingredient ratios had the following fermentation product analysis results: polypeptide content of 17.8%, live lactic acid bacteria count of 2.14 × 10^8 CFU / g, and protease activity of 605 U / g.

[0029] Feeding trials on piglets showed that the average daily weight gain was 1.84 kg, the diarrhea rate decreased by 65%, and the feed utilization rate increased by 12%.

[0030] Example 3: This invention provides a technical solution: a polypeptide feed, which is composed of the following raw materials by mass percentage: 37% crude plant extract, 38% protein source, 8% microbial flora, and 17% fermentation auxiliary material.

[0031] The plant extract is composed of the following raw materials in the following proportions by mass percentage: 50% crude extract of Astragalus membranaceus, 30% crude extract of Codonopsis pilosula, and 20% crude extract of Angelica sinensis.

[0032] The protein source is composed of the following ingredients in the following proportions by weight: 70% soy protein isolate and 30% fish meal.

[0033] The microbial community is composed of the following raw materials in the following proportions by mass percentage: Bacillus subtilis 40%, Bacillus licheniformis 20%, Saccharomyces cerevisiae 20%, and Lactobacillus plantarum 20%.

[0034] The fermentation adjuvant is composed of the following raw materials in the following proportions by mass percentage: 90% brown sugar powder, 9% protein powder, 0.5% dipotassium hydrogen phosphate, 0.3% magnesium sulfate, and 0.2% compound vitamins.

[0035] The specific production method of the polypeptide feed of the present invention is as follows: S1. Raw material pretreatment: Mix soybean meal and fish meal in proportion, grind to 80 mesh, sterilize at 121℃ for 20 minutes, mix plant crude extract in proportion, and mix with protein source at a mass ratio of 1:2, and adjust the moisture content to 50%. S2. Inoculation and Fermentation of Compound Strains: Bacillus subtilis was cultured on LB medium at 37°C for 12 hours, Bacillus licheniformis on TSB medium at 37°C for 20 hours, Saccharomyces cerevisiae on YPD medium at 28°C for 18 hours, and Lactobacillus plantarum on MRS medium at 37°C for 24 hours. The activated bacterial solutions were then mixed in a specific ratio (Bacillus subtilis: Bacillus licheniformis: Saccharomyces cerevisiae: Lactobacillus plantarum = 4:2:2:2), with a total inoculation amount of 5%. Fermentation was then carried out under the following conditions: temperature 30±1°C, pH 6.8, aeration rate 1.5 L / min, and fermentation time 48 hours. During fermentation, the pH was monitored every 12 hours, and glucose (0.5%) was added to maintain the pH at 6.5-7.0. Free amino acids were added simultaneously with the protein source during fermentation to convert the protein into tetrapeptides. S3. Post-processing and finished product preparation: After fermentation, the cells are inactivated by heat treatment at 80℃ for 10 minutes, followed by spray drying (controlling the inlet air temperature to 180℃ and the outlet air temperature to 80℃) or low-temperature drying (45℃ to moisture content ≤8%).

[0036] The feed produced using the above-mentioned ingredient ratios had the following fermentation product analysis results: polypeptide content of 18.5%, live lactic acid bacteria count of 2.23 × 10^8 CFU / g, and protease activity of 612 U / g.

[0037] Feeding trials on piglets showed that the average daily weight gain was 1.89 kg, the diarrhea rate decreased by 67%, and the feed utilization rate increased by 12.6%.

[0038] The dosage and usage of the polypeptide feed of this invention are as follows: Each bottle is produced in 1000ml form. Dilute with water at a ratio of 60-100, i.e., each bottle is diluted with 120-200 jin of water. The diluted peptide energy water is given once a day, with each pig drinking about 3 jin each time. Alternatively, the diluted peptide energy water can be mixed with the feed, with 1 jin of water for every 2 jin of feed. It should be noted that the drinking water must not contain bleach or disinfectant, and it must not be used in combination with antibiotics. When using antibiotics, there should be an interval of at least one hour.

[0039] This invention is rich in various plant and animal-derived amino acids, which can supplement various nutrients in the animal body, maintain amino acid balance, promote protein and fat absorption, promote muscle and fat growth, and improve meat flavor. At the same time, the peptide-rich feed contains various beneficial bacteria and free fatty acids, which cause vasoactive intestinal peptides to dilate blood vessels in the mesentery, stimulate intestinal fluid and gastrointestinal motility, and increase the secretion of proteases and digestive enzymes. This strengthens intestinal motility, enhances the body's digestion, absorption, and anabolism, thereby promoting growth. It can effectively solve the problem of excessive erythrocytes in piglets, giving the skin a rosy and shiny appearance, significantly increasing serum immunoglobulins, improving the immune function and growth and development of piglets, promoting blood circulation, preventing anemia, increasing the activity of probiotics in the oral and nasal mucosa, and inhibiting the risk of African swine fever virus infection. The overall polypeptide feed is green, safe, and residue-free, containing no chemical or prohibited ingredients, which can reduce the burden on the animal's body, improve health, and has good effects.

[0040] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A polypeptide feed, characterized in that: The polypeptide feed is composed of the following raw materials in percentage by mass: plant crude extract 35%-40%, protein source 35%-45%, microbial flora 5%-8%, and fermentation auxiliary materials 15%-20%.

2. A polypeptide feed according to claim 1, characterised in that: The plant extract is composed of the following raw materials in percentage by mass: crude extract of Astragalus membranaceus 50%, crude extract of Codonopsis pilosula 30%, and crude extract of Angelica sinensis 20%.

3. The polypeptide feed of claim 1, wherein: The protein source is composed of the following raw materials in percentage by mass: soybean protein isolate 70% and fish meal 30%.

4. The polypeptide feed of claim 1, wherein: The microbial flora is composed of the following raw materials in percentage by mass: Bacillus subtilis 40%, Bacillus licheniformis 20%, Saccharomyces cerevisiae 20%, and Lactobacillus plantarum 20%.

5. The polypeptide feed of claim 1, wherein: The fermentation auxiliary materials are composed of the following raw materials in percentage by mass: yellow sugar powder 90%, protein powder 9%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.3%, and compound vitamins 0.2%.