Concentration and purification device and method for preparing fructus cannabis functional peptide

Abstract

The invention relates to a concentration and purification device and method for preparing fructus cannabis functional peptide, belongs to the technical field of devices for preparing peptide, and aims to solve the background problems that in the prior art, when peptide is separated and purified from raw materials, impurities in the raw materials easily block a filter membrane, and two methods are generally adopted for solving the blocking problem: one method needs to clean the filter membrane, and the other method needs to clean the filter membrane; the other filter membrane needs to be replaced; the problem that the efficiency of separating and purifying the polypeptide is greatly reduced due to the fact that the filter membrane can be replaced only after the introduction of raw materials is stopped when the filter membrane is cleaned or replaced and the separation and purification work of the polypeptide needs to be interrupted is solved. The method comprises the following steps: opening a feeding pipe valve, pouring raw materials through a feeding pipe, and enabling the raw materials to fall on a semipermeable membrane; the raw materials are concentrated and purified through semipermeable membrane filtration, and the purified raw materials fall to the bottom of the reaction barrel to be collected; the purified raw materials can be taken out by opening a discharge port valve; the booster pump is electrified to pressurize the interior of the reaction barrel, so that the speed of the raw materials passing through the semipermeable membrane can be accelerated.

Description

Technical Field

[0001] This invention belongs to the technical field of peptide preparation apparatus, and in particular to a concentration and purification apparatus and method for preparing hemp seed functional peptides. Background Technology

[0002] Hemp seed functional peptides are small molecule peptides with specific physiological activities prepared from hemp seeds through technologies such as bio-enzymatic hydrolysis.

[0003] In existing technologies for separating and purifying peptides from raw materials, the presence of impurities in the raw materials can easily clog the filter membrane during the separation process, preventing the raw materials from passing through. There are generally two methods to solve this clogging problem: one is to clean the filter membrane, and the other is to replace the filter membrane. Regardless of whether you clean or replace the filter membrane, you need to stop the flow of raw materials before you can replace the filter membrane. This interrupts the peptide separation and purification process, which greatly reduces the efficiency of peptide separation and purification. Summary of the Invention

[0004] The purpose of this invention is to solve the above-mentioned problems existing in the prior art and to provide a concentration and purification device and method for preparing hemp seed functional peptides.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A concentration and purification device for preparing hemp seed functional peptides includes: a reaction device, a purification device, and an anti-blocking device. The purification device is fixed inside the reaction device, and the anti-blocking device is detachably and fixedly connected to the purification device.

[0007] The reaction apparatus includes: a reaction tank, a discharge port and a cover assembly. The bottom of the reaction tank is provided with a discharge port, and a valve is provided on the discharge port. The upper end of the reaction tank is detachably and securely sealed with a cover assembly by bolts and nuts.

[0008] The cover assembly includes: a cover, a feed pipe, a drive motor, a friction plate, and a booster pump. The cover is detachably fixed to the upper end of the reaction vessel by bolts and nuts. The cover is provided with a feed pipe and a valve. The cover is fixed with a drive motor, the output shaft of which rotates through the cover and is fixed with a friction plate. The cover is fixed with a booster pump, the outlet of which is fixedly inserted into the cover.

[0009] The purification device includes: a fixed ring, a fixed ring groove, a screw, a movable plate, a wing nut, a cylindrical ring, a support protrusion, a support plate, a connecting ring, a connecting ring groove, and a semi-permeable membrane. A fixed ring is fixed to the inner wall of the reaction tank. A fixed ring groove is provided on the top surface of the fixed ring. Screws are symmetrically fixed to the fixed ring. A movable plate is slidably connected to each screw. A wing nut is screwed onto each screw. A cylindrical ring is fixed to the lower end of the two movable plates. The diameter of the cylindrical ring matches the diameter of the fixed ring groove. A support protrusion is provided circumferentially at the upper end of the inner ring of the fixed ring. Multiple support plates are evenly distributed and fixed to the inner side of the fixed ring. A connecting ring is fixed between the multiple support plates. A connecting ring groove is provided on the top surface of the connecting ring. The semi-permeable membrane is annular. One end of the semi-permeable membrane rests on the fixed ring groove. The top of the cylindrical ring rests against the semi-permeable membrane. The semi-permeable membrane rests on the support protrusion. The inner ring of the semi-permeable membrane rests on the connecting ring.

[0010] The anti-clogging device includes: a rotating shaft, a first nut, a sliding plate, a bracket, a scraper, a spring baffle, a first spring, a sealed bearing, a fixed column, a second nut, a pressure plate, a limiting unit, and a second friction plate. The rotating shaft has threads on its surface. The first nut is screwed onto the rotating shaft. The sliding plate is slidably connected to the rotating shaft. Multiple brackets are evenly distributed and fixed around the periphery of the sliding plate. Each bracket has a scraper rotatably connected to its lower end. A spring baffle is fixed on the rotating shaft. A first spring is fitted onto the rotating shaft, positioned between the spring baffle and the sliding plate. The first nut rests against the sliding plate. The pressure plate rests against the semi-permeable membrane. The lower end of the rotating shaft is rotatably connected to the fixed column via a sealed bearing. The outer wall of the fixed column has threads. The second nut is screwed into the fixed post, and the pressure plate is slidably connected to the fixed post. The bottom surface of the pressure plate is provided with an annular protrusion, the diameter of which matches the groove of the connecting ring. The pressure plate rests against the connecting ring, and the annular protrusion rests against the semi-permeable membrane. The semi-permeable membrane is disposed in the groove of the connecting ring. Multiple limiting units are evenly distributed and fixed at the lower end of the fixed post. The limiting units rest against the bottom surface of the connecting ring. A second friction plate is fixed on the rotating shaft, and the second friction plate is in contact with the first friction plate.

[0011] Each of the aforementioned limiting units includes: a fixed tube, a fixed tube hole, a sliding rod, and a second spring. One end of the fixed tube is fixedly connected to a fixed post. The fixed tube has symmetrical fixed tube holes arranged along its length. The sliding rod slides into the fixed tube. The sliding rod has symmetrically arranged protruding rods. The two protruding rods slide out of the two fixed tube holes respectively. A second spring is arranged inside the fixed tube.

[0012] A method for using a concentration and purification apparatus for preparing functional peptides from hemp seeds, the method comprising the following steps:

[0013] S1. Open the feed pipe valve and pour the raw material in through the feed pipe. The raw material falls onto the semi-permeable membrane.

[0014] S2. The raw material is concentrated and purified by semi-permeable membrane filtration, and the purified raw material falls to the bottom of the reaction tank for collection.

[0015] S3. Open the discharge valve to remove the purified raw material;

[0016] S4. Powering on the booster pump pressurizes the inside of the reaction vessel, which can accelerate the speed at which the raw materials pass through the semi-permeable membrane.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] This invention provides a concentration and purification device for preparing functional peptides from hemp seeds, which can solve the problem mentioned in the background: In the prior art, when separating and purifying peptides from raw materials, the presence of impurities in the raw materials easily clogs the filter membrane during the separation process, preventing the raw materials from passing through the filter membrane. There are generally two methods to solve the above-mentioned clogging problem: one is to clean the filter membrane, and the other is to replace the filter membrane. Regardless of whether cleaning or replacing the filter membrane, the raw material supply must be stopped before the filter membrane can be replaced. The separation and purification of peptides needs to be interrupted, which will greatly reduce the efficiency of peptide separation and purification. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0020] Figure 2 This is a schematic diagram of the purification device.

[0021] Figure 3 Top view of the connecting ring;

[0022] Figure 4 This is a magnified view of a portion of the purification apparatus;

[0023] Figure 5 Top view of the fixed ring;

[0024] Figure 6 This is a top view of a cylindrical ring;

[0025] Figure 7 This is a schematic diagram of the anti-clogging device.

[0026] Figure 8 This is a magnified view of a portion of the anti-blocking device;

[0027] Figure 9 This is a schematic diagram of the limiting unit structure. Detailed Implementation

[0028] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0029] Please see Figure 1-9 The present invention provides a technical solution: a concentration and purification device for preparing hemp seed functional peptides, comprising: a reaction device 1, a purification device 2 and an anti-blocking device 3, wherein the purification device 2 is fixed inside the reaction device 1, and the anti-blocking device 3 is detachably and fixedly connected to the purification device 2.

[0030] The reaction device 1 includes: a reaction tank 1-1, a discharge port 1-2, and a cover assembly 1-3. The bottom of the reaction tank 1-1 is provided with a discharge port 1-2, and a valve is provided on the discharge port 1-2. The upper end of the reaction tank 1-1 is detachably and securely fixed with a cover assembly 1-3 by bolts and nuts.

[0031] The cover assembly 1-3 includes: cover 1-3-1, feed pipe 1-3-2, drive motor 1-3-3, friction plate 1-3-4, and booster pump 1-3-5. The cover 1-3-1 is detachably fixed to the upper end of the reaction vessel 1-1 by bolts and nuts. The feed pipe 1-3-2 is provided on the cover 1-3-1, and valve 2 is provided on the feed pipe 1-3-2. The drive motor 1-3-3 is fixed on the cover 1-3-1. The output shaft of the drive motor 1-3-3 rotates through the cover 1-3-1 and is fixed to the friction plate 1-3-4. The booster pump 1-3-5 is fixed on the cover 1-3-1, and the air outlet of the booster pump 1-3-5 is fixedly inserted into the cover 1-3-1.

[0032] The purification device 2 includes: a fixed ring 2-1, a fixed ring groove 2-2, a screw 2-3, a movable plate 2-4, a wing nut 2-5, a cylindrical ring 2-6, a support protrusion 2-7, a support plate 2-8, a connecting ring 2-9, a connecting ring groove 2-10, and a semi-permeable membrane 2-11. A fixed ring 2-1 is fixed to the inner wall of the reaction tank 1-1. A fixed ring groove 2-2 is provided on the top surface of the fixed ring 2-1. Screws 2-3 are symmetrically fixed to the fixed ring 2-1. A movable plate 2-4 is slidably connected to each screw 2-3. A wing nut 2-5 is screwed onto each screw 2-3. Cylindrical rings are fixed to the lower ends of the two movable plates 2-4. 2-6, the diameter of the cylindrical ring 2-6 matches the diameter of the fixed ring groove 2-2, the upper end of the inner ring of the fixed ring 2-1 is provided with a support protrusion 2-7 along its circumference, a plurality of support plates 2-8 are evenly distributed and fixed on the inner side of the fixed ring 2-1, a connecting ring 2-9 is fixed in the middle of the plurality of support plates 2-8, the top surface of the connecting ring 2-9 is provided with a connecting ring groove 2-10, the semi-permeable membrane 2-11 is set as an annular shape, one end of the semi-permeable membrane 2-11 is placed on the fixed ring groove 2-2, the cylindrical ring 2-6 rests on the top of the semi-permeable membrane 2-11, the semi-permeable membrane 2-11 is placed on the support protrusion 2-7, and the inner ring of the semi-permeable membrane 2-11 is placed on the connecting ring 2-9.

[0033] The anti-blocking device 3 includes: a rotating shaft 3-1, a nut 3-2, a sliding plate 3-3, a bracket 3-4, a scraper 3-5, a spring baffle 3-6, a spring 3-7, a sealed bearing 3-8, a fixed column 3-9, a second nut 3-10, a pressure plate 3-11, a limiting unit 3-12, and a second friction plate 3-13. The rotating shaft 3-1 has threads on its surface. The first nut 3-2 is screwed into the rotating shaft 3-1. The sliding plate 3-3 is slidably connected to the rotating shaft 3-1. Multiple brackets are evenly distributed and fixed around the periphery of the sliding plate 3-3. 3-4, each of the brackets 3-4 has a scraper 3-5 rotatably connected to its lower end. A spring baffle 3-6 is fixed on the rotating shaft 3-1. A spring 3-7 is fitted onto the rotating shaft 3-1, and the spring 3-7 is positioned between the spring baffle 3-6 and the sliding plate 3-3. A nut 3-2 abuts against the sliding plate 3-3. A pressure plate 3-11 rests against the semi-permeable membrane 2-11. The lower end of the rotating shaft 3-1 is rotatably connected to a fixed column 3-9 via a sealed bearing 3-8. The outer wall of the fixed column 3-9 is threaded. Nut 3-10 is screwed into fixed post 3-9. Pressure plate 3-11 is slidably connected to fixed post 3-9. The bottom surface of pressure plate 3-11 is provided with an annular protrusion, the diameter of which matches the connecting ring groove 2-10. Pressure plate 3-11 rests against connecting ring 2-9, and the annular protrusion rests against semi-permeable membrane 2-11. Semi-permeable membrane 2-11 is disposed in connecting ring groove 2-10. Multiple limiting units 3-12 are evenly distributed and fixed at the lower end of fixed post 3-9. Limiting units 3-12 rest against the bottom surface of connecting ring 2-9. Friction plate 3-13 is fixed on rotating shaft 3-1. Friction plate 3-13 is in contact with friction plate 1-3-4.

[0034] Each of the multiple limiting units 3-12 includes: a fixed tube 3-12-1, a fixed tube hole 3-12-2, a sliding rod 3-12-3, and a second spring 3-12-4. One end of the fixed tube 3-12-1 is fixedly connected to the fixed post 3-9. The fixed tube 3-12-1 has fixed tube holes 3-12-2 symmetrically arranged along its length. The sliding rod 3-12-3 slides into the fixed tube 3-12-1. The sliding rod 3-12-3 has protruding rods symmetrically arranged on it. The two protruding rods slide out of the two fixed tube holes 3-12-2 respectively. The second spring 3-12-4 is arranged inside the fixed tube 3-12-1.

[0035] A method for using a concentration and purification apparatus for preparing functional peptides from hemp seeds, the method comprising the following steps:

[0036] S1. Open valve 1-3-2 of feed pipe and pour the raw material in through feed pipe 1-3-2. The raw material falls onto semi-permeable membrane 2-11.

[0037] S2. The raw material is concentrated and purified by filtration through a semi-permeable membrane 2-11. The purified raw material falls to the bottom of the reaction tank 1-1 for collection.

[0038] S3. Opening valves 1-2 at the discharge port allows the purified raw material to be removed.

[0039] S4. Power on booster pump 1-3-5 to pressurize the inside of reaction tank 1-1, which can accelerate the speed at which raw materials pass through semi-permeable membrane 2-11.

[0040] The working principle of this invention is as follows:

[0041] When using this device, open valve 1-3-2 of the feed pipe to pour the raw material in. The raw material falls onto the semi-permeable membrane 2-11 and is concentrated and purified by filtration through the semi-permeable membrane 2-11. The purified raw material falls to the bottom of the reaction tank 1-1 for collection. Opening valve 1-2 of the outlet allows the purified raw material to be removed. Powering on the booster pump 1-3-5 pressurizes the inside of the reaction tank 1-1, accelerating the speed at which the raw material passes through the semi-permeable membrane 2-11. The booster pump 1-3-5 is a prior art device. The technique utilizes externally purchased components. The semi-permeable membrane 2-11 is fixed using the annular groove 2-2, cylindrical ring 2-6, connecting annular groove 2-10, and annular protrusions at the bottom of the pressure plate 3-11. This keeps the semi-permeable membrane 2-11 taut, maximizing its contact with the raw material and increasing its efficiency. Furthermore, the supporting protrusions 2-7 allow the semi-permeable membrane 2-11 to be aligned with the connecting ring 2-10. -9 is inclined in a conical shape, which helps to converge the raw materials and prevents them from overflowing through the periphery. It also reduces the centrifugal force generated on the raw materials when the scraper 3-5 rotates, further preventing overflow. The pressure plate 3-11 also blocks the raw material liquid, preventing it from overflowing through the semi-permeable membrane 2-11. This device is connected to an external power source, which powers the drive motor 1-3-3 to rotate the friction plate 1-3-4. The rotation of the friction plate 1-3-4 drives the friction plate 2-13 to rotate, which in turn drives the rotating shaft 3-1. The rotating shaft 3-1 rotates, which drives the scraper 3-5 to rotate via the sliding plate 3-3 and the support 3-4. The rotating scraper 3-5 scrapes away impurities or filter residue above the semi-permeable membrane 2-11, which can prevent impurities or filter residue from accumulating and clogging the semi-permeable membrane 2-11. The sliding plate 3-3 is pressed against the nut 3-2 by the spring 3-7, which clamps the scraper 3-5, making it easier for the rotating shaft 3-1 to drive the sliding plate 3-3 to rotate. In the above way, hemp seed functional peptides can be continuously concentrated and purified.When maintenance is required after a production cycle or when the semi-permeable membrane 2-11 reaches its service life and needs replacement, remove the bolts and nuts securing the cover 1-3-1, remove the cover 1-3-1, and rotate nut 3-2 to move it upwards along the rotating shaft 3-1. Simultaneously, spring 3-7 causes the sliding plate 3-3 to move upwards, thus separating the scraper 3-5 from the semi-permeable membrane 2-11. Then, rotate nut 3-10 to move it upwards along the fixed post 3-9, thus removing the pressure plate 3-11 from the connecting ring 2-9. Then, pull the protruding rod inwards along the fixed tube hole 3-12-2, thereby moving the sliding rod 3-12-3 into the fixed tube 3-12-1. Simultaneously, spring 3-12-4 is compressed, and spring 3-12-4 exerts pressure on the sliding rod. 3-12-3 serves as a reset mechanism. The fixed tube hole 3-12-2 and the protruding rod limit the movement of the sliding rod 3-12-3. The sliding rod 3-12-3 no longer moves against the bottom surface of the connecting ring 2-9. At this point, the anti-blocking device 3 can be removed for thorough cleaning and maintenance. Then, rotate the wing nut 2-5 to move it upwards along the screw 2-3, separating the cylindrical ring 2-6 from the fixed ring groove 2-2. The semi-permeable membrane 2-11 can then be removed. The purification device 2 can then be thoroughly cleaned and maintained. After maintenance, a new semi-permeable membrane 2-11 is placed on the fixed ring 2-1. Reversing the above steps fixes and resets the semi-permeable membrane 2-11, allowing for the next concentration and purification production cycle.

[0042] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0043] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0044] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of the equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0045] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A concentrated purification device for preparing hemp seed functional peptides, characterized in that: Include: Reaction device (1), purification device (2) and prevent blocking device (3), the reaction device (1) inside fixed with purification device (2), the prevent blocking device (3) and purification device (2) can be detachably fixedly connected.

2. A device for concentrating and purifying hemp seed functional peptides according to claim 1, characterized in that: The reaction device (1) includes: reaction bucket (1-1), discharge port (1-2) and lid assembly (1-3), the reaction bucket (1-1) bottom is provided with discharge port (1-2), the discharge port (1-2) is provided with valve one, the reaction bucket (1-1) upper end is detachably fixed with lid assembly (1-3) through bolt nut sealing.

3. The device for concentrating and purifying hemp seed functional peptides according to claim 2, characterized in that: The lid assembly (1-3) includes: lid (1-3-1), feed pipe (1-3-2), drive motor (1-3-3), friction plate one (1-3-4) and booster pump (1-3-5), the lid (1-3-1) is detachably fixed with the upper end of the reaction bucket (1-1) through bolt nut sealing, the lid (1-3-1) is provided with feed pipe (1-3-2), the feed pipe (1-3-2) is provided with valve two, the lid (1-3-1) is fixed with drive motor (1-3-3), the output shaft of drive motor (1-3-3) rotates through the lid (1-3-1) and is fixed with friction plate one (1-3-4), the lid (1-3-1) is fixed with booster pump (1-3-5), the outlet end of booster pump (1-3-5) is fixed into the lid (1-3-1).

4. The device for concentrating and purifying hemp seed functional peptides according to claim 2, characterized in that: Said purification device (2) includes: fixed ring (2-1), fixed ring groove (2-2), screw one (2-3), moving plate (2-4), butterfly nut (2-5), cylindrical ring (2-6), support protrusion (2-7), support plate (2-8), connecting ring (2-9), connecting ring groove (2-10) and semi-permeable membrane (2-11), the fixed ring (2-1) is fixed in the inner wall of the reaction bucket (1-1), the top surface of the fixed ring (2-1) is provided with the fixed ring groove (2-2), the fixed ring (2-1) is fixed symmetrically with screw one (2-3) on it, one moving plate (2-4) is connected on each screw one (2-3) in a sliding manner, one butterfly nut (2-5) is connected in a screwing manner on each screw one (2-3), two moving plates (2-4) are fixed with cylindrical ring (2-6) at the lower end, the diameter of the cylindrical ring (2-6) matches the diameter of the fixed ring groove (2-2), the inner ring of the fixed ring (2-1) is provided with support protrusion (2-7) along its circumferential direction on the upper end, a plurality of support plates (2-8) are fixed on the inner side of the fixed ring (2-1), a connecting ring (2-9) is fixed in a plurality of support plates (2-8), the connecting ring (2-9) is provided with connecting ring groove (2-10) on the top surface, the semi-permeable membrane (2-11) is arranged in a ring shape, one end of the semi-permeable membrane (2-11) is arranged on the fixed ring groove (2-2), the cylindrical ring (2-6) is arranged above the semi-permeable membrane (2-11), the semi-permeable membrane (2-11) is arranged on the support protrusion (2-7), and the inner ring of the semi-permeable membrane (2-11) is arranged on the connecting ring (2-9).

5. The device for concentrating and purifying hemp seed functional peptides according to claim 4, characterized in that: The anti-blocking device (3) comprises a rotating shaft (3-1), a nut (3-2), a sliding plate (3-3), a support (3-4), a scraper (3-5), a spring baffle (3-6), a spring (3-7), a sealing bearing (3-8), a fixed column (3-9), a nut (3-10), a pressing plate (3-11), a limiting unit (3-12) and a friction plate (3-13), the rotating shaft (3-1) is provided with threads on the surface, the nut (3-2) is rotatably connected with the rotating shaft (3-1), the sliding plate (3-3) is slidably connected with the rotating shaft (3-1), a plurality of supports (3-4) are uniformly arranged on the circumferential side of the sliding plate (3-3), and each support (3-4) is rotatably connected with a scraper (3-5) at the lower end, the spring baffle (3-6) is fixed on the rotating shaft (3-1), the spring (3-7) is sleeved on the rotating shaft (3-1), the spring (3-7) is arranged between the spring baffle (3-6) and the sliding plate (3-3), the nut (3-2) abuts against the sliding plate (3-3), the pressing plate (3-11) abuts against the semi-permeable membrane (2-11) from above, the lower end of the rotating shaft (3-1) is rotatably connected with the fixed column (3-9) through the sealing bearing (3-8), the fixed column (3-9) is provided with threads on the outer wall, the nut (3-10) is rotatably connected with the fixed column (3-9), the pressing plate (3-11) is slidably connected with the fixed column (3-9), the bottom surface of the pressing plate (3-11) is provided with an annular protrusion, the diameter of the annular protrusion matches the connecting ring groove (2-10), the pressing plate (3-11) abuts against the connecting ring (2-9), the annular protrusion abuts against the semi-permeable membrane (2-11), the semi-permeable membrane (2-11) is arranged in the connecting ring groove (2-10), a plurality of limiting units (3-12) are uniformly arranged on the lower end of the fixed column (3-9), the limiting units (3-12) abut against the bottom surface of the connecting ring (2-9), and the friction plate (3-13) is fixed on the rotating shaft (3-1).

6. The device for concentrating and purifying hemp seed functional peptides according to claim 5, characterized in that: Each of the limiting units (3-12) comprises a fixed tube (3-12-1), a fixed tube hole (3-12-2), a sliding rod (3-12-3) and a spring (3-12-4), one end of the fixed tube (3-12-1) is fixedly connected with the fixed column (3-9), the fixed tube (3-12-1) is provided with the fixed tube hole (3-12-2) on the length direction thereof, the sliding rod (3-12-3) is slidably inserted into the fixed tube (3-12-1), the sliding rod (3-12-3) is provided with protruding rods, and the two protruding rods are slidably inserted into the two fixed tube holes (3-12-2) respectively, the spring (3-12-4) is arranged in the fixed tube (3-12-1).

7. A method for using the concentrated purification device for preparing the functional peptide of Cannabis sativa L. according to any one of claims 1-6, characterized in that: The method comprises the following steps: S1, open the feed pipe (1-3-2) valve, pour the raw material through the feed pipe (1-3-2), the raw material falls on the semi-permeable membrane (2-11); S2, the raw material is filtered and concentrated by the semi-permeable membrane (2-11), and the purified raw material falls on the bottom of the reaction bucket (1-1) for collection; S3, open the discharge port (1-2) valve to take out the purified raw material; S4, power on the booster pump (1-3-5) to pressurize the inside of the reaction bucket (1-1), which can accelerate the speed of the raw material passing through the semi-permeable membrane (2-11).

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