Campylobacter jejuni constant-temperature culture device

Abstract

The invention discloses a campylobacter jejuni constant-temperature culture device, and relates to the technical field of microorganism culture, the campylobacter jejuni constant-temperature culture device comprises a moving frame and a culture box connected to the top of the moving frame, the top of the moving frame is further connected with a gas buffer cylinder, the gas buffer cylinder is connected with at least three gas inlet cylinders, and the gas inlet cylinders are connected with the moving frame. The gas inlet cylinder is positioned below the gas buffer cylinder and is communicated with the gas buffer cylinder; the three gas inlet cylinders are connected into an O gas source, a CO gas source and an N gas source respectively, the elastic force of a first reset spring in each gas inlet cylinder is adjusted by means of a rotating bolt to preset a leading-in threshold value, a sealing plate is pushed to slide after the gas pressure reaches the standard, and the gas is quantitatively led into a buffer cylinder through a gas inlet pipe; the gas flows around through staggered through holes of the heating plate, so that the contact time with the heating resistance wire is prolonged for full preheating; and finally, a sealing plate drives a transmission rack to unlock a mounting shaft, a coil spring drives a tuning fork stirring frame to rotate and cooperates with an impact column and a connecting spring to generate high-frequency vibration, and the gas mixing and preheating effect is improved.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, specifically to a constant temperature culture device for Campylobacter jejuni. Background Technology

[0002] Campylobacter jejuni, a common foodborne pathogen, requires isolation and culture as a core step in food safety testing, epidemiological investigation, and research into its pathogenic mechanisms. The growth of this bacterium demands stringent environmental conditions: a precise gas mixture of 5% O2 + 10% CO2 + 85% N must be maintained, and the culture temperature must be stable at 42℃ ± 1℃. Furthermore, the gas mixture must be evenly distributed within the incubator to avoid localized deviations in the gas ratio or temperature fluctuations that could inhibit bacterial growth and reduce isolation efficiency.

[0003] Existing devices typically use heating wires on the inner wall of the incubator or a heating plate at the bottom for temperature control. After gas is introduced, it directly enters the culture chamber, which can easily lead to temperature differences between the inlet and the outlet due to localized airflow impact. Furthermore, the gas heating and mixing processes are separated, lacking a dedicated buffer heating structure. The introduced low-temperature gas can easily cause temperature fluctuations within the incubator, affecting the metabolic stability of Campylobacter jejuni.

[0004] Furthermore, in the process of gas introduction, existing technologies often introduce mixed gas directly through premixed gas cylinders. This method cannot adapt to the ratio compensation after gas consumption in real time, and long-distance transportation or changes in cylinder pressure can easily lead to ratio drift, which in turn affects the uniformity of strain growth.

[0005] To address these issues, we designed a constant-temperature culture device for Campylobacter jejuni. Summary of the Invention

[0006] The purpose of this invention is to provide a constant temperature culture device for Campylobacter jejuni to solve the problems mentioned in the background art.

[0007] To solve the above-mentioned technical problems, the present invention provides a constant temperature culture device for Campylobacter jejuni, comprising a movable frame and an incubator connected to the top of the movable frame. A gas buffer cylinder is also connected to the top of the movable frame. At least three air inlets are connected to the gas buffer cylinder, and the air inlets are located below the gas buffer cylinder and communicate with it. A sealing plate is slidably connected inside the air inlet cylinder. A first return spring is connected to one side of the sealing plate. At least three heating plates are connected inside the gas buffer cylinder. The heating plates have through holes for gas mixing and built-in heating resistance wires for preheating the gas.

[0008] Furthermore, an installation shaft is rotatably connected inside the gas buffer cylinder, and a coil spring is provided at the bottom of the gas buffer cylinder. One end of the coil spring is connected to the inner wall of the gas buffer cylinder, and the other end is connected to the installation shaft. A tuning fork stirrer for mixing the gas is connected to the top of the installation shaft.

[0009] Furthermore, a limiting disk is coaxially provided on the mounting shaft, and the limiting disk has at least three limiting slots. At least three limiting blocks are slidably connected inside the gas buffer cylinder, and the positions of the limiting blocks correspond to the limiting slots.

[0010] Furthermore, a first transmission rack is connected to the end of the sealing plate away from the first return spring, and a driven gear is meshed on the first transmission rack. A connecting shaft is rotatably connected inside the gas buffer cylinder, and the driven gear is connected to the connecting shaft. A second transmission rack is meshed on the other side of the driven gear, and one side of the second transmission rack is connected to the limiting block.

[0011] Furthermore, an electromagnet is connected inside the gas buffer cylinder, and a second return spring is connected to the other side of the electromagnet. The other end of the second return spring is connected to the limiting block.

[0012] Furthermore, a fixed plate is connected inside the gas buffer cylinder, and a guide block is connected to the top of the limiting block, with the guide block slidably connected inside the fixed plate.

[0013] Furthermore, at least two symmetrical connecting springs are connected inside the gas buffer cylinder, and the other end of the connecting spring is connected to an impact column. The impact column corresponds to the fork arm of the tuning fork stirrer and is used to generate vibration by collision when the tuning fork stirrer rotates to enhance gas mixing.

[0014] Furthermore, the heating plates are evenly spaced along the axial direction of the gas buffer cylinder, and the through holes of adjacent heating plates are staggered to prolong the contact time between the gas and the heating plates and promote gas mixing.

[0015] Furthermore, a rotating bolt is threaded onto the air intake cylinder, and a mounting plate is rotatably connected to the other end of the rotating bolt. The end of the first return spring away from the sealing plate is connected to the mounting plate, and an air intake pipe is connected to the air intake cylinder.

[0016] Furthermore, the incubator is equipped with a vacuum pump, the suction end of which is connected to a gas buffer cylinder, and the exhaust end extends into the incubator and is connected to an exhaust pipe.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. First, connect the three air inlets to the O2, CO2, and N2 gas sources respectively. Adjust the spring force of the first reset spring in each air inlet to the preset inlet threshold using the rotating bolt. After the gas pressure reaches the standard, push the sealing plate to slide and introduce the gas into the buffer cylinder through the inlet pipe in a metered manner. Then, make the gas flow around through the staggered through-hole of the heating plate to extend the contact time with the heating resistance wire and fully preheat it. Finally, the sealing plate drives the transmission rack to unlock the installation shaft, and the coil spring drives the tuning fork stirring frame to rotate. In conjunction with the impact column and the connecting spring, high-frequency vibration is generated to improve the gas mixing and preheating effect.

[0019] 2. If the gas ratio deviates during cultivation, rotate the rotating bolt of the corresponding air inlet cylinder to change the elasticity of the first reset spring and adjust the gas introduction threshold to compensate for the ratio deviation in real time. After cultivation, energize the electromagnet to attract the limiting block, compress the second reset spring to make it disengage from the limiting plate, manually rotate the mounting shaft to store the spring force, and after power is cut off, the second reset spring rebounds to push the limiting block to re-lock. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural diagram of the overall external structure of the present invention;

[0021] Figure 2 This is a rear view of the present invention;

[0022] Figure 3 This is a first sectional view of the present invention;

[0023] Figure 4 This is a second sectional view of the present invention;

[0024] Figure 5 This is a third sectional view of the present invention;

[0025] Figure 6 For the present invention Figure 3 Enlarged view of point A in the middle;

[0026] Figure 7 For the present invention Figure 4 Enlarged view of point B in the middle;

[0027] Figure 8 For the present invention Figure 4 Enlarged view of point C in the middle.

[0028] In the diagram: 1. Moving frame; 2. Incubator; 3. Gas buffer cylinder; 4. Air inlet cylinder; 5. Heating plate; 6. Mounting shaft; 7. Tuning fork stirrer; 8. Coil spring; 9. Mounting plate; 10. First return spring; 11. Sealing plate; 12. Limiting plate; 13. Limiting block; 14. First transmission rack; 15. Driven gear; 16. Connecting shaft; 17. Second transmission rack; 18. Electromagnet; 19. Second return spring; 20. Fixing plate; 21. Guide block; 22. Connecting spring; 23. Impact column; 24. Rotating bolt; 25. Air inlet pipe; 26. Air outlet pipe. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] Please see Figure 1-8 The present invention provides a technical solution: a constant temperature culture device for Campylobacter jejuni, comprising a movable frame 1 and a culture box 2 connected to the top of the movable frame 1. A gas buffer cylinder 3 is also connected to the top of the movable frame 1. At least three air inlets 4 are connected to the gas buffer cylinder 3, and the air inlets 4 are located below the gas buffer cylinder 3 and communicate with it. A sealing plate 11 is slidably connected inside the air inlet cylinder 4. A first return spring 10 is connected to one side of the sealing plate 11. At least three heating plates 5 are connected inside the gas buffer cylinder 3. The heating plates 5 have through holes for gas mixing and built-in heating resistance wires for preheating the gas.

[0031] It should be noted that the three air inlets 4 can be used to introduce O2, CO2, and N2 respectively, and the elastic coefficient of the first return spring 10 in each air inlet 4 is different. Among them, the spring force is the highest for N2 and the lowest for CO2.

[0032] In practice, three air inlets 4 are connected to O2, CO2, and N2 gas sources respectively, and the three gases enter their respective air inlets 4. Because the spring force coefficient of the first return spring 10 in each air inlet 4 is different, the gas needs to accumulate a sufficient amount to generate pressure until it overcomes the spring force of the corresponding first return spring 10. When the pressure of a single gas reaches the standard, the sealing plate 11 is pushed to slide towards the gas buffer cylinder 3 to open the air inlet channel. A fixed amount of gas is introduced into the gas buffer cylinder 3 through the opened channel. The gas flows through the heating plate 5 in the buffer cylinder. The heating plate 5 has a built-in resistance wire that heats up the gas, and at the same time, the gas is initially mixed through the through hole of the heating plate 5.

[0033] See Figure 1-8A mounting shaft 6 is rotatably connected inside the gas buffer cylinder 3. A coil spring 8 is provided at the bottom of the gas buffer cylinder 3. One end of the coil spring 8 is connected to the inner wall of the gas buffer cylinder 3, and the other end is connected to the mounting shaft 6. A tuning fork stirring frame 7 for mixing the gas is connected to the top of the mounting shaft 6.

[0034] In practice, before starting the device, manually rotate the mounting shaft 6 to stretch and store the spring 8, keeping it taut. After storing the power, the spring 8 will not release the power temporarily due to the subsequent locking structure. When the locking structure is unlocked, the spring 8 will rebound and release the power, driving the mounting shaft 6 to rotate. The mounting shaft 6 will drive the tuning fork stirring frame 7 at the top to rotate in the buffer cylinder, stirring the mixed gas flowing through the heating plate 5.

[0035] See Figure 1-8 A limiting disk 12 is coaxially mounted on the mounting shaft 6. The limiting disk 12 has at least three limiting slots. At least three limiting blocks 13 are slidably connected inside the gas buffer cylinder 3. The positions of the limiting blocks 13 correspond to the limiting slots.

[0036] In practice, when the coil spring 8 is charging, the limiting block 13 aligns with the limiting slot of the limiting plate 12 and is inserted into the slot, restricting the rotation of the limiting plate 12 and the mounting shaft 6. When the subsequent linkage structure drives the limiting block 13 to move, the limiting block 13 disengages from the limiting slot. The limiting plate 12 is released from restriction and rotates synchronously with the mounting shaft 6, and the coil spring 8 releases its power.

[0037] See Figure 1-8 The sealing plate 11 is connected to a first transmission rack 14 at one end away from the first return spring 10. A driven gear 15 is meshed on the first transmission rack 14. A connecting shaft 16 is rotatably connected inside the gas buffer cylinder 3. The driven gear 15 is connected to the connecting shaft 16. A second transmission rack 17 is meshed on the other side of the driven gear 15. One side of the second transmission rack 17 is connected to the limiting block 13.

[0038] In practice, when the gas pressure in the air inlet cylinder 4 reaches the standard, it pushes the sealing plate 11 to move towards the buffer cylinder. The sealing plate 11 drives the connected first transmission rack 14 to slide synchronously. The first transmission rack 14 meshes with the driven gear 15, driving the driven gear 15 to rotate around the connecting shaft 16. The driven gear 15 meshes with the second transmission rack 17, driving the second transmission rack 17 to slide away from the limiting plate 12. The second transmission rack 17 drives the limiting block 13 to disengage from the slot of the limiting plate 12, and then the coil spring 8 can release the power.

[0039] See Figure 1-8 An electromagnet 18 is connected inside the gas buffer cylinder 3. A second reset spring 19 is connected to the other side of the electromagnet 18. The other end of the second reset spring 19 is connected to the limit block 13.

[0040] It should be noted that the limiting block 13 is a magnetic block or a metal block.

[0041] In practice, after stirring is completed and gas is introduced into the incubator 2, the electromagnet 18 is energized, and the electromagnet 18 generates magnetic force; the magnetic force attracts the limiting block 13 to move towards the electromagnet 18, compressing the second reset spring 19, and the limiting block 13 disengages from the limiting plate 12; the mounting shaft 6 is manually rotated to re-stretch and store force on the coil spring 8; after the force is stored, the power supply to the electromagnet 18 is disconnected, the second reset spring 19 rebounds, and pushes the limiting block 13 back into the slot of the limiting plate 12.

[0042] See Figure 1-8 A fixed plate 20 is connected inside the gas buffer cylinder 3, and a guide block 21 is connected to the top of the limiting block 13. The guide block 21 is slidably connected inside the fixed plate 20.

[0043] In practice, the guide block 21 can limit and guide the movement of the limit block 13, further improving the stability of the limit block 13 during the movement process.

[0044] See Figure 1-8 The gas buffer cylinder 3 is connected to at least two symmetrical connecting springs 22. The other end of the connecting spring 22 is connected to an impact column 23. The impact column 23 corresponds to the fork arm of the tuning fork stirrer 7 and is used to generate vibration by collision when the tuning fork stirrer 7 rotates to enhance gas mixing.

[0045] In practice, when the tuning fork stirring frame 7 rotates, its fork arm periodically impacts the impact column 23 inside the buffer cylinder; after being impacted, the impact column 23 moves toward the connecting spring 22 and compresses the connecting spring 22.

[0046] After the connecting spring 22 is compressed to its limit, it rebounds and pushes the impact column 23 to strike the fork arm of the tuning fork mixer 7 in the opposite direction. While the tuning fork mixer 7 is rotating, it generates high-frequency micro-vibrations due to repeated impacts, which drives the gas in the buffer cylinder to turbulence and further improve the gas mixing effect.

[0047] See Figure 1-8 The heating plates 5 are evenly spaced along the axis of the gas buffer cylinder 3, and the through holes of adjacent heating plates 5 are staggered to prolong the contact time between the gas and the heating plates 5 and promote gas mixing.

[0048] In practice, after a metered amount of gas is introduced into the buffer cylinder, it flows toward the heating plate 5. Because the through holes of adjacent heating plates 5 are misaligned, the gas cannot pass through in a straight line and needs to flow around the through holes. The heating plate 5 has a built-in resistance wire that generates heat, and the flow around the through holes prolongs the contact time between the gas and the heating plate 5.

[0049] See Figure 1-8The air intake cylinder 4 is threaded with a rotating bolt 24, and the other end of the rotating bolt 24 is rotatably connected to a mounting plate 9. The end of the first return spring 10 away from the sealing plate 11 is connected to the mounting plate 9, and the air intake cylinder 4 is connected to an air intake pipe 25.

[0050] In practice, if a gas ratio deviation is detected during the cultivation process, the rotating bolt 24 on the corresponding air inlet cylinder 4 is rotated, and the bolt moves inward or outward along the thread of the air inlet cylinder 4. The rotating bolt 24 pushes or pulls the connected mounting plate 9, changing the pre-compression of the first reset spring 10, thereby adjusting the spring force of the first reset spring 10. After the spring force changes, the corresponding gas introduction pressure threshold changes, thus achieving gas ratio compensation. The gas is then directly introduced into the heating area through the air inlet pipe 25.

[0051] See Figure 1-8 An air pump is connected to the incubator 2. The air pump's suction end is connected to the gas buffer cylinder 3, and the exhaust end extends into the incubator 2 and is connected to an exhaust pipe 26.

[0052] In practice, the vacuum pump is started, and its suction end draws mixed gas from the buffer cylinder; the exhaust end of the vacuum pump sends the gas into the exhaust pipe 26; the serpentine exhaust pipe 26 diffuses the gas evenly in the incubator 2 through multi-directional openings, covering the culture medium.

[0053] Working principle: Before use, manually rotate the mounting shaft 6 inside the gas buffer cylinder 3 to stretch the coil spring 8 and store power; the limiting plate 12 coaxial with the mounting shaft 6 engages with the limiting block 13 inside the buffer cylinder, locking the power of the coil spring 8. Connect the three air inlets 4 to O2, CO2, and N2 gas sources respectively. Adjust the spring force of the first reset spring 10 by rotating the bolt 24 on the air inlet cylinder 4, preset the gas introduction threshold, and the gas accumulates in the air inlet cylinder 4. After the pressure reaches the standard, it pushes the sealing plate 11 to slide and open the channel; a metered amount of gas is introduced into the heating zone of the buffer cylinder through the air inlet pipe 25.

[0054] The mixed gas flows through the heating plates 5 that are evenly distributed inside the buffer cylinder. Because the through holes of adjacent heating plates 5 are misaligned, the gas flows around them, extending the contact time with the heating resistance wire.

[0055] Subsequently, the sealing plate 11 drives the first transmission rack 14 to slide, meshing with the driven gear 15 to rotate, which in turn drives the second transmission rack 17 to pull the limit block 13 away from the limit plate 12, unlocking the mounting shaft 6. The coil spring 8 releases power, driving the mounting shaft 6 and the tuning fork stirring frame 7 to rotate. The stirring frame fork arm strikes the impact column 23, compresses the connecting spring 22, and then rebounds to strike in the opposite direction, causing the tuning fork stirring frame 7 to generate high-frequency vibration, enhancing gas mixing. If the gas ratio deviates, the rotating bolt 24 of the corresponding air inlet cylinder 4 is rotated to change the elasticity of the first reset spring 10, adjust the gas introduction threshold, compensate for the ratio deviation, start the air pump, draw mixed gas from the buffer cylinder, and diffuse it evenly in the incubator 2 through the serpentine air outlet pipe 26 to cover the culture medium.

[0056] After cultivation, the electromagnet 18 is energized, which attracts the limiting block 13 and compresses the second reset spring 19, disengaging it from the limiting plate 12. The mounting shaft 6 is manually rotated to store the power of the coil spring 8. After the power is cut off, the second reset spring 19 rebounds, and the limiting block 13 re-engages.

Claims

1. A constant temperature culture device for Campylobacter jejuni, comprising a movable frame (1) and an incubator (2) connected to the top of the movable frame (1), characterized in that, The top of the mobile frame (1) is also connected to a gas buffer cylinder (3). At least three air inlets (4) are connected to the gas buffer cylinder (3), and the air inlets (4) are located below the gas buffer cylinder (3) and communicate with each other. A sealing plate (11) is slidably connected inside the air inlet cylinder (4). A first return spring (10) is connected to one side of the sealing plate (11). At least three heating plates (5) are connected inside the gas buffer cylinder (3). The heating plates (5) have through holes for gas mixing and built-in heating resistance wires for preheating the gas.

2. The constant temperature culture device for Campylobacter jejuni as described in claim 1, characterized in that: The gas buffer cylinder (3) is rotatably connected to an installation shaft (6). A coil spring (8) is provided at the bottom of the gas buffer cylinder (3). One end of the coil spring (8) is connected to the inner wall of the gas buffer cylinder (3), and the other end is connected to the installation shaft (6). A tuning fork stirrer (7) for mixing the gas is connected to the top of the installation shaft (6).

3. The constant temperature culture device for Campylobacter jejuni as described in claim 2, characterized in that: A limiting disk (12) is coaxially arranged on the mounting shaft (6). At least three limiting slots are opened on the limiting disk (12). At least three limiting blocks (13) are slidably connected inside the gas buffer cylinder (3). The positions of the limiting blocks (13) correspond to the limiting slots.

4. The constant temperature culture device for Campylobacter jejuni as described in claim 1, characterized in that: The sealing plate (11) is connected to a first transmission rack (14) at one end away from the first return spring (10). A driven gear (15) is meshed on the first transmission rack (14). A connecting shaft (16) is rotatably connected inside the gas buffer cylinder (3). The driven gear (15) is connected to the connecting shaft (16). A second transmission rack (17) is meshed on the other side of the driven gear (15). One side of the second transmission rack (17) is connected to the limiting block (13).

5. The constant temperature culture device for Campylobacter jejuni as described in claim 3, characterized in that: An electromagnet (18) is connected inside the gas buffer cylinder (3). A second reset spring (19) is connected to the other side of the electromagnet (18). The other end of the second reset spring (19) is connected to the limiting block (13).

6. The constant temperature culture device for Campylobacter jejuni as described in claim 3, characterized in that: The gas buffer cylinder (3) is connected to a fixed plate (20), and the top of the limiting block (13) is connected to a guide block (21), which is slidably connected to the fixed plate (20).

7. The constant temperature culture device for Campylobacter jejuni as described in claim 2, characterized in that: The gas buffer cylinder (3) is connected to at least two symmetrical connecting springs (22). The other end of the connecting spring (22) is connected to an impact column (23). The impact column (23) corresponds to the fork arm of the tuning fork stirrer (7) and is used to generate vibration by collision when the tuning fork stirrer (7) rotates to enhance gas mixing.

8. The constant temperature culture device for Campylobacter jejuni as described in claim 1, characterized in that: The heating plates (5) are evenly spaced along the axial direction of the gas buffer cylinder (3), and the through holes of adjacent heating plates (5) are staggered to prolong the contact time between the gas and the heating plates (5) and promote gas mixing.

9. The constant temperature culture device for Campylobacter jejuni as described in claim 1, characterized in that: The air inlet cylinder (4) is threaded with a rotating bolt (24), and the other end of the rotating bolt (24) is rotatably connected to a mounting plate (9). The end of the first reset spring (10) away from the sealing plate (11) is connected to the mounting plate (9). The air inlet cylinder (4) is connected with an air inlet pipe (25).

10. The constant temperature culture device for Campylobacter jejuni as described in claim 1, characterized in that: The incubator (2) is connected to a vacuum pump. The vacuum pump's suction end is connected to the gas buffer cylinder (3), and the exhaust end extends into the incubator (2) and is connected to an exhaust pipe (26).

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