A treatment method for promoting rhizome growth during harvest of polygonatum franchetii

Abstract

The present application provides a kind of processing method for promoting rhizome growth when yunnan rhizoma polygonati is harvested, and relates to yunnan rhizoma polygonati planting technical field.The processing method is to use the nutrient base fertilizer prepared by biochar, manure, straw cooking material and commelina fermenting material to supplement fertilizer, and use the foliar fertilizer prepared by commelina sterilization liquid, ethyl isothiocyanate, potassium dihydrogen phosphate, rice vinegar and humic acid to carry out foliar fertilization.The present application overcomes the shortcomings of prior art, can effectively ensure the quality of yunnan rhizoma polygonati and promote the growth of yunnan rhizoma polygonati root, so as to achieve the purpose of high yield, and improve the economic benefit of yunnan rhizoma polygonati planting.

Description

Technical Field

[0001] This invention relates to the field of Solomon's seal cultivation technology, specifically to a treatment method for promoting rhizome growth during harvesting of Solomon's seal. Background Technology

[0002] Polygonatum yunnanense is a herbaceous plant belonging to the genus Polygonatum in the family Asparagaceae, and is a characteristic resource plant of Yunnan Province. Its main economic part is the stem tuber. Polygonatum yunnanense is a traditional resource that can be developed and utilized for both medicinal and edible purposes. Modern pharmacological research results show that it has good effects in lowering blood sugar, anti-oxidation, anti-aging, anti-exercise fatigue, and protecting the cardiovascular system.

[0003] In recent years, high market demand has led to excessive consumption of wild resources, resulting in widespread cultivation. Therefore, research into key technologies such as the cultivation of Polygonatum odoratum varieties, resource development, and precise harvesting is essential. Furthermore, with the increasing conversion of arable land to non-grain crops, and given the long cultivation and harvesting period of Polygonatum odoratum in Yunnan (Yunnan), improving its yield and quality is of paramount importance. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a treatment method to promote rhizome growth during the harvesting of Polygonatum yunnanense. This method effectively ensures the quality of Polygonatum yunnanense while promoting root growth, thereby achieving high yield and improving the economic benefits of Polygonatum yunnanense cultivation.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A treatment method to promote rhizome growth during harvesting of Polygonatum yunnanense, the treatment method comprising the following steps:

[0007] S1. Prepare the nutrient base fertilizer according to the following weight proportions: 8-10 parts biochar, 2-6 parts manure, 3-6 parts straw cooking material, and 1-2 parts dayflower fermented material; wherein the dayflower fermented material is obtained by fermenting dayflower with Nocardia lutea.

[0008] S2. Prepare foliar fertilizer according to the following mass proportions: 20-30 parts of Commelina communis fermented sterilized liquid, 0.1-0.2 parts of ethyl isothiocyanate, 15-20 parts of potassium dihydrogen phosphate, 8-10 parts of rice vinegar, and 8-10 parts of humic acid; the Commelina communis fermented sterilized liquid is obtained by sterilizing the bacterial liquid separated by pressing after fermentation of Commelina communis with Nocardia lutea.

[0009] S3. Base fertilizer application and management: In the autumn of the year before harvest, apply nutrient base fertilizer to the roots of the Yunnan Polygonatum seedlings for the first time, and then apply nutrient base fertilizer every 3-4 months thereafter.

[0010] S4. Foliar fertilizer application management: Apply foliar fertilizer for the first time in the spring of the harvest year, and then apply foliar fertilizer for the second time at an interval of 40-60 days, until July-August of the same year, when foliar fertilizer is applied every 15 days.

[0011] Preferably, the method for preparing the straw cooking feed includes the following steps:

[0012] S1-1. Cut the straw into sections and dry it to constant weight to obtain dried straw material;

[0013] S1-2. Place the dried straw material in 3-5 times its volume of clean water and boil it at 90-100℃ for 20-30 minutes. Then cool and dry it, and then crush it and pass it through a 20-mesh sieve to obtain the boiled straw material.

[0014] Preferably, the preparation method of the *Commelina communis* fermented feed and the *Commelina communis* fermentation sterilization liquid includes the following steps:

[0015] S2-1. Take fresh dayflower, rinse it with clean water, add 5-7 times its volume of clean water and grind it into a paste to obtain dayflower paste for later use.

[0016] S2-2. Inoculate Nocardia cambogia into Commelina communis slurry and ferment for 12-24 hours to obtain fermented material for later use.

[0017] S2-3. Press and filter the fermented material to separate the filter residue and filtrate. Dry the filter residue with microwave to obtain the fermented material of Commelina communis.

[0018] S2-4. Sterilize the filtrate at 120℃ for 15 minutes to obtain the fermented sterilized dayflower broth.

[0019] Preferably, in step S2-2, the inoculation amount of Nocardia lutea is 0.1%-0.3% of the total mass of the Commelina communis slurry, and the bacterial content of Nocardia lutea is 10. 8 -10 10 per g.

[0020] Preferably, the power of microwave drying in steps S2-3 is 280W.

[0021] Preferably, the amount of fertilizer applied to the roots in step S3 is 400-600 kg / mu, and the soil is thoroughly watered after fertilization.

[0022] Preferably, in step S3, fertilization is carried out in the evening when the ambient temperature is above 15℃, and at noon when the ambient temperature is below 15℃.

[0023] Preferably, in step S4, the foliar fertilizer should be diluted 100-300 times before being sprayed onto the leaves of *Polygonatum yunnanense*, and the amount sprayed should be such that the leaves are covered with water droplets but do not drip.

[0024] Preferably, the foliar fertilizer is applied a total of 5-6 times in step S4.

[0025] This invention provides a treatment method to promote rhizome growth during the harvesting of Polygonatum yunnanense, which has the following advantages compared with the prior art:

[0026] This invention uses biochar, manure, boiled straw, and fermented dayflower as a base fertilizer, and applies supplementary fertilizer starting in the autumn of the year before the harvest of Polygonatum yunnanense. This effectively promotes rapid root growth of Polygonatum yunnanense with a small amount of fertilizer, effectively promoting root enlargement and achieving high yield. Furthermore, the foliar fertilizer prepared by combining fermented and sterilized dayflower as a base fertilizer with ethyl isothiocyanate, potassium dihydrogen phosphate, rice vinegar, and humic acid can effectively ensure the quality of the harvested Polygonatum yunnanense and comprehensively improve the economic benefits of artificial cultivation. Attached Figure Description

[0027] Figure 1 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 2 in an embodiment of the present invention;

[0028] Figure 2 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 3 in an embodiment of the present invention;

[0029] Figure 3 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 4 in an embodiment of the present invention;

[0030] Figure 4 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 5 in an embodiment of the present invention;

[0031] Figure 5 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 6 in an embodiment of the present invention;

[0032] Figure 6 This is a schematic diagram comparing the tubers of Polygonatum yunnanense in planting area 1 and planting area 7 in an embodiment of the present invention. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] The *Nocardia lutea* strain used in the following examples has the accession number CGMCC No. 16927. This strain was disclosed in a prior patent application with application number CN201811609808.4, and the viable count of this strain is 10. 9 The following manure is composted sheep manure; the following straw is corn stalk; and the biochar is rice husk charcoal. Example 1

[0035] Raw material preparation:

[0036] 1. Preparation of straw cooking feed:

[0037] (1) After cutting the straw into sections, dry it at 45°C to constant weight to obtain dried straw material;

[0038] (2) Place the dried straw material in 4 times the amount of clean water and boil it at 95°C for 25 minutes. Then cool and dry it, and then crush it and pass it through a 20-mesh sieve to obtain the straw boiled material.

[0039] 2. Crushed straw:

[0040] (1) After cutting the straw into sections, dry it at 45°C to constant weight, crush it and pass it through a 20-mesh sieve to obtain straw crushed material.

[0041] 3. Preparation of Commelina communis fermented feed and Commelina communis fermented sterilized liquid:

[0042] (1) After rinsing the fresh dayflower with clean water, add 6 times the volume of clean water and grind it into a paste to obtain dayflower paste for later use;

[0043] (2) Inoculate 0.2% of the total mass of Nocardia cambogia into the Commelina communis slurry and ferment for 18 hours (28℃) to obtain fermented material for later use;

[0044] (3) Press and filter the fermented material to separate the filter residue and the filtrate. The filter residue is dried by microwave at 280W to obtain the fermented material of dayflower. The filtrate is sterilized at 120℃ for 15 minutes to obtain the sterilized fermented liquid of dayflower.

[0045] 4. Preparation of Commelina communis fermentation broth:

[0046] (1) After rinsing the fresh dayflower with clean water, add 6 times the volume of clean water and grind it into a paste to obtain dayflower paste for later use;

[0047] (2) Inoculate 0.2% of the total mass of Nocardia cambogia into the Commelina communis slurry and ferment for 18 hours (28℃) to obtain fermented material for later use;

[0048] (3) Press and filter the fermented material to separate the filter residue and the filtrate. The filtrate is the fermented liquid of Commelina communis.

[0049] 5. Preparation of dayflower feed and dayflower extract:

[0050] (1) After rinsing the fresh dayflower with clean water, add 6 times the volume of clean water and grind it into a paste to obtain dayflower paste for later use;

[0051] (2) Press and filter the dayflower pulp to separate the filter residue and filtrate. Dry the filter residue with a 280W microwave to obtain dayflower pulp. Sterilize the filtrate at 120℃ for 15 minutes to obtain dayflower extract.

[0052] 6. Prepare the nutrient base fertilizer and foliar fertilizer according to the mass proportions of each raw material in Table 1 below:

[0053] Table 1

[0054] Example 2

[0055] Field planting experiment of Polygonatum yunnanense:

[0056] 1. Test site:

[0057] A three-year-old *Polygonatum yunnanense* plantation in Weiyuan Town, Jinggu Dai and Yi Autonomous County, Pu'er City, Yunnan Province, was selected as the experimental site. The plantation was divided into seven planting areas, designated as Planting Area 1-7, with each area spaced 50-80 meters apart, maintaining a shading rate of 50%-60%.

[0058] 2. Experimental field management:

[0059] Different nutrient base fertilizers and foliar fertilizers were used to manage the fertilization of Polygonatum yunnanensis in various planting areas:

[0060] Nutrient base fertilizer (500 kg / mu) was applied to the roots of *Polygonatum yunnanense* in each planting area on September 10, 2024, December 27, 2024, April 6, 2025, and July 20, 2025, respectively. When the ambient temperature was above 15℃, fertilization was carried out in the evening, and when the ambient temperature was below 15℃, fertilization was carried out at noon. After fertilization, the soil was thoroughly watered.

[0061] Foliar fertilizer will be sprayed on each planting area of ​​Polygonatum yunnanensis on March 12, 2025, April 25, 2025, June 20, 2025, July 30, 2025 and August 14, 2025 respectively (the amount of spraying should be such that the leaves are covered with water droplets but do not drip).

[0062] The specific selection of base fertilizer and foliar fertilizer for each planting area is shown in Table 2 below:

[0063] Table 2

[0064]

[0065] On September 12, 2025, the Yunnan Polygonatum was harvested in the field. Ten Yunnan Polygonatum plants were randomly selected from each planting area. The growth of the root tubers and the fresh weight of the harvested tubers were recorded in each planting area. The specific results are shown in Table 3 below:

[0066] Table 3

[0067]

[0068] As shown in the table above, the addition of boiled straw and fermented dayflower grass to the nutrient base fertilizer A used in planting area 1 significantly promoted the development of rhizomes of Polygonatum yunnanense and increased its yield. Comparative photos of harvested rhizomes from each group are shown below. Figure 1-6 As shown (larger pieces of tuber were selected from each plant for comparison).

[0069] The nutritional components (polysaccharide content, saponin content, and total flavonoid content) of the harvested Polygonatum yunnanense from each group were analyzed, and the specific results are shown in Table 4 below.

[0070] Table 4

[0071]

[0072] As shown in the table above, the quality of the Polygonatum yunnanense harvested in planting area 1 was the best. In planting area 6, the addition of ethyl isothiocyanate to the foliar fertilizer was reduced, resulting in a significant decrease in the final total flavonoid and saponin content compared to planting area 1.

[0073] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A treatment method for promoting rhizome growth during the harvesting of Polygonatum yunnanense, characterized in that, The processing method includes the following steps: S1. Prepare the nutrient base fertilizer according to the following weight proportions: 8-10 parts biochar, 2-6 parts manure, 3-6 parts straw cooking material, and 1-2 parts dayflower fermented material; wherein the dayflower fermented material is obtained by fermenting dayflower with Nocardia lutea. S2. Prepare foliar fertilizer according to the following mass proportions: 20-30 parts of Commelina communis fermented sterilized liquid, 0.1-0.2 parts of ethyl isothiocyanate, 15-20 parts of potassium dihydrogen phosphate, 8-10 parts of rice vinegar, and 8-10 parts of humic acid; the Commelina communis fermented sterilized liquid is obtained by sterilizing the bacterial liquid separated by pressing after fermentation of Commelina communis with Nocardia lutea. S3. Base fertilizer application and management: In the autumn of the year before harvest, apply nutrient base fertilizer to the roots of the Yunnan Polygonatum seedlings for the first time, and then apply nutrient base fertilizer every 3-4 months thereafter. S4. Foliar fertilizer application management: Apply foliar fertilizer for the first time in the spring of the harvest year, and then apply foliar fertilizer for the second time at an interval of 40-60 days, until July-August of the same year, when foliar fertilizer is applied every 15 days.

2. The processing method according to claim 1, characterized in that, The preparation method of the straw cooking feed includes the following steps: S1-1. Cut the straw into sections and dry it to constant weight to obtain dried straw material; S1-2. Place the dried straw material in 3-5 times its volume of clean water and boil it at 90-100℃ for 20-30 minutes. Then cool and dry it, and then crush it and pass it through a 20-mesh sieve to obtain the boiled straw material.

3. The processing method according to claim 1, characterized in that, The preparation method of the Dayflower fermented feed and Dayflower fermented sterilized liquid includes the following steps: S2-1. Take fresh dayflower, rinse it with clean water, add 5-7 times its volume of clean water and grind it into a paste to obtain dayflower paste for later use. S2-2. Inoculate Nocardia cambogia into Commelina communis slurry and ferment for 12-24 hours to obtain fermented material for later use. S2-3. Press and filter the fermented material to separate the filter residue and filtrate. Dry the filter residue with microwave to obtain the fermented material of Commelina communis. S2-4. Sterilize the filtrate at 120℃ for 15 minutes to obtain the fermented sterilized dayflower broth.

4. The processing method according to claim 3, characterized in that: In step S2-2, the inoculation amount of Nocardia cambogia is 0.1%-0.3% of the total mass of the Commelina communis slurry, and the bacterial content of Nocardia cambogia is 10. 8 -10 10 per g.

5. The processing method according to claim 3, characterized in that: The power of microwave drying in step S2-3 is 280W.

6. The processing method according to claim 1, characterized in that: In step S3, the amount of fertilizer applied to the roots in a single application is 400-600 kg / mu, and the soil should be thoroughly watered after fertilization.

7. The processing method according to claim 1, characterized in that: In step S3, fertilization is carried out in the evening when the ambient temperature is above 15℃, and at noon when the ambient temperature is below 15℃.

8. The processing method according to claim 1, characterized in that: In step S4, the foliar fertilizer should be diluted 100-300 times before being sprayed onto the leaves of *Polygonatum yunnanense*. The amount of fertilizer applied should be such that the leaves are covered with water droplets but do not drip.

9. The processing method according to claim 1, characterized in that: In step S4, the foliar fertilizer is applied a total of 5-6 times.

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