Plant extract composite bacteriostatic agent and application thereof

Abstract

The invention discloses a plant extract composite bacteriostatic agent and application thereof. The plant extract composite bacteriostatic agent is prepared from Japan cypress leaf extract, raspberry seed extract and gardenia fruit extract. According to the plant extract composite bacteriostatic agent and the preparation method thereof, the synergistic interaction among the extracts is fully exerted, the antibacterial and antioxidant effects can be remarkably improved, the purpose of rapid and long-acting bacteriostasis can be achieved, and the plant extract composite bacteriostatic agent can be used in oil wax-based products such as eye shadow and lip glaze and has the advantages of being low in dosage, safe, effective, natural, low in irritation and the like.

Description

Technical Field

[0001] This invention belongs to the technical field of plant extract compound antibacterial agents, and particularly relates to a plant extract compound antibacterial agent and its application. Background Technology

[0002] In recent years, with the improvement of living standards, people have paid more and more attention to skin care, and the use of cosmetics and skin care products has become more frequent. Preservatives are an indispensable ingredient in cosmetics. Adding preservatives can inhibit the growth of microorganisms in cosmetics, and choosing efficient and safe preservatives is one of the keys to cosmetic formulation development.

[0003] Traditionally, cosmetics, personal care compositions, and cleaning compositions often employ one or more of the 51 preservatives permitted by cosmetic regulations in combination to address the problem of microbial growth. These 51 preservatives include phenols, esters, halides, and quaternary ammonium salts, with parabens, isothiazolinones, imidazolidinyl ureas, and bromopol being the most commonly used. However, chemical preservatives can easily cause skin problems; for example, isothiazolinones can cause irritation and allergies, and bromopol may lead to the formation of carcinogens. Therefore, many countries have imposed strict limits on their types and dosages. Traditional chemical preservatives can no longer meet people's needs; therefore, the development of natural extract preservatives is becoming increasingly important. Summary of the Invention

[0004] To address the shortcomings of the prior art, this invention provides a plant extract composite antibacterial agent. The extracts in this plant extract composite antibacterial agent have a synergistic effect, which can significantly improve the antibacterial and antioxidant effects and achieve rapid and long-lasting antibacterial effect. This plant extract composite antibacterial agent can be used in oil and wax-based products such as eyeshadow and lip gloss, and has the advantages of low dosage, safety and effectiveness, and natural low irritation.

[0005] The purpose of this invention is to provide a compound antibacterial agent of plant extracts, including: Japanese cypress leaf extract, Raspberry seed extract, and Gardenia fruit extract.

[0006] In some embodiments of the present invention, the mass ratio of the Japanese cypress leaf extract, the raspberry seed extract and the gardenia fruit extract is 1~2:4~7:3~5; including but not limited to 1:4:3, 1:7:3, 1:4:5, 1:7:5, 2:4:3, 2:7:3, 2:4:5, and 2:7:5.

[0007] In some embodiments of the present invention, the extraction process of the Japanese cypress leaf extract includes the following steps:

[0008] Japanese cypress leaves were pulverized, and the powder was extracted with ethanol-water solution and ultrasonically extracted. The extract was filtered and concentrated to obtain Japanese cypress leaf extract.

[0009] In some embodiments of the present invention, the mass ratio of Japanese cypress leaves to ethanol aqueous solution is 1:5 to 8, including but not limited to 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, and 1:8.

[0010] In some embodiments of the present invention, the mass concentration of the ethanol aqueous solution is 50-80%, including but not limited to 50%, 55%, 60%, 65%, 70%, 75%, and 80%.

[0011] In some embodiments of the present invention, the extraction temperature is 50~60°C and the extraction time is 1~3 hours.

[0012] In some embodiments of the present invention, the filtration temperature is 50~60°C.

[0013] In some embodiments of the present invention, the extraction process of the Rubus idaeus seed extract includes the following steps:

[0014] The seeds of Rubus xinganensis were crushed, and the powder was extracted with ethanol-water solution. The extract was purified, filtered, concentrated, and then adsorbed, eluted, and dried through macroporous resin to obtain Rubus xinganensis seed extract.

[0015] In some embodiments of the present invention, the mass ratio of the Rhizoma Rubus dahuricae seeds to the aqueous ethanol solution is 1:6 to 10, including but not limited to 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, and 1:10.

[0016] In some embodiments of the present invention, the mass concentration of the ethanol aqueous solution is 60-80%, including but not limited to 60%, 65%, 70%, 75%, and 80%.

[0017] In some embodiments of the present invention, the extraction temperature is 50~70℃ and the extraction time is 2~6 hours.

[0018] In some embodiments of the present invention, the purification is performed by decolorization with activated carbon.

[0019] In some embodiments of the present invention, the filtration temperature is 50~70°C.

[0020] In some embodiments of the present invention, the elution solvent is 60-70 wt% ethanol.

[0021] In some embodiments of the present invention, the extraction process of the gardenia fruit extract includes the following steps:

[0022] Gardenia fruit was crushed, and the gardenia fruit powder was extracted by heating and reflux with an ethanol aqueous solution. The extract was concentrated, and the concentrate was adsorbed, eluted, and dried through a macroporous resin to obtain gardenia fruit extract.

[0023] In some embodiments of the present invention, the mass ratio of gardenia fruit to ethanol aqueous solution is 1:8 to 10, including but not limited to 1:8, 1:8.5, 1:9, 1:9.5, and 1:10.

[0024] In some embodiments of the present invention, the mass concentration of the ethanol aqueous solution is 60-80%, including but not limited to 60%, 65%, 70%, 75%, and 80%.

[0025] In some embodiments of the present invention, the extraction temperature is 40~60℃ and the extraction time is 2~6 hours.

[0026] In some embodiments of the present invention, the elution solvent is 60-70 wt% ethanol.

[0027] Another object of the present invention is to provide the application of the aforementioned plant extract compound antibacterial agent in the preparation of cosmetics, wherein the cosmetics contain the aforementioned plant extract compound antibacterial agent.

[0028] In some embodiments of the present invention, the cosmetic is an oil-wax-based product.

[0029] In some embodiments of the present invention, the cosmetic includes at least one of foundation cream, lip gloss, eyeshadow, and blush.

[0030] In some embodiments of the present invention, the amount of the plant extract compound antibacterial agent, by mass percentage, is 0.5-5% of the cosmetic, including but not limited to 0.5%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, 2.3%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 4.8%, and 5.0%.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) This invention significantly enhances the antibacterial effect by fully leveraging the synergistic effect among Japanese cypress leaf extract, Rhus chinensis seed extract and gardenia fruit extract.

[0033] (2) This invention significantly enhances the antioxidant effect by fully leveraging the synergistic effect among Japanese cypress leaf extract, Rhus chinensis seed extract and gardenia fruit extract.

[0034] (3) The plant extract compound antibacterial agent of the present invention can be used in oil and wax-based products such as eyeshadow and lip gloss, and has the advantages of low dosage, safety and effectiveness, and natural low irritation. Detailed Implementation

[0035] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0036] Example 1

[0037] This embodiment provides an extraction process for Japanese cypress leaf extract, the specific extraction process is as follows:

[0038] Take 56 kg of Japanese cypress leaves, pulverize them using a traditional Chinese medicine pulverizer, and pass them through an 80-mesh sieve to obtain Japanese cypress leaf powder. Take 50 kg of the sieved Japanese cypress leaf powder, add 5 times the amount of 80wt% ethanol aqueous solution, mix evenly, and then extract for 2 hours at a temperature of 55℃. After extraction, transfer the extract mixture to an ultrasonic extraction device and extract it for 2 hours using a high-power dual-frequency ultrasonic device. After extraction, filter the extract to remove impurities, and remove water and ethanol at 55℃. The remaining Japanese cypress leaf extract is further purified by ultrafiltration to obtain Japanese cypress leaf extract.

[0039] Example 2

[0040] This embodiment provides an extraction process for Japanese cypress leaf extract, the specific extraction process is as follows:

[0041] Take 56 kg of Japanese cypress leaves, pulverize them using a traditional Chinese medicine pulverizer, and pass them through an 80-mesh sieve to obtain Japanese cypress leaf powder. Take 50 kg of the sieved Japanese cypress leaf powder, add 8 times the amount of 50%wt ethanol aqueous solution, mix evenly, and then extract for 3 hours at a temperature of 50℃. After extraction, transfer the extract mixture to an ultrasonic extraction device and extract it for 2 hours using a high-power dual-frequency ultrasonic device. After extraction, filter the extract to remove impurities, and remove water and ethanol at 60℃. The remaining Japanese cypress leaf extract is further purified by ultrafiltration to obtain Japanese cypress leaf extract.

[0042] Example 3

[0043] This embodiment provides an extraction process for Japanese cypress leaf extract, the specific extraction process is as follows:

[0044] Take 56 kg of Japanese cypress leaves, pulverize them using a traditional Chinese medicine pulverizer, and pass them through an 80-mesh sieve to obtain Japanese cypress leaf powder. Take 50 kg of the sieved Japanese cypress leaf powder, add 6 times the amount of 60wt% ethanol aqueous solution, mix evenly, and then extract for 2.5 hours at a temperature of 60℃. After extraction, transfer the extract mixture to an ultrasonic extraction device and extract it for 2 hours using a high-power dual-frequency ultrasonic device. After extraction, filter the extract to remove impurities, and remove water and ethanol at 50℃. Further purify the remaining Japanese cypress leaf extract by ultrafiltration to obtain Japanese cypress leaf extract.

[0045] Example 4

[0046] This embodiment provides an extraction process for extracting Raspberry seeds from Xingan, and the specific extraction process is as follows:

[0047] Take 56 kg of Rubus idaeus seeds, pulverize them using a traditional Chinese medicine pulverizer, and then pass them through an 80-mesh sieve for later use. Take 50 kg of the sieved Rubus idaeus seed powder and add it to an extraction container. Add 6 times the amount of 80 wt% ethanol aqueous solution, and extract three times with ultrasound at 50℃ and normal pressure, 1 hour each time. Combine the extracts, add 5 wt% activated carbon, heat and reflux to decolorize for 0.5 hours, filter while hot, and concentrate under reduced pressure at 65℃ to obtain Rubus idaeus seed extract concentrate. The concentrate is adsorbed by macroporous resin and eluted with 70 wt% ethanol. The eluent is spray-dried to obtain Rubus idaeus seed extract.

[0048] Example 5

[0049] This embodiment provides an extraction process for extracting Raspberry seeds from Xingan, and the specific extraction process is as follows:

[0050] Take 56 kg of Rubus idaeus seeds, pulverize them using a traditional Chinese medicine pulverizer, and then pass them through an 80-mesh sieve for later use. Take 50 kg of the sieved Rubus idaeus seed powder and add it to an extraction container. Add 10 times the amount of 60 wt% ethanol aqueous solution and extract twice with ultrasound at 70℃ and normal pressure, 2 hours each time. Combine the extracts, add 1 wt% activated carbon, heat and reflux for decolorization for 2 hours, filter while hot, and concentrate under reduced pressure at 65℃ to obtain Rubus idaeus seed extract concentrate. The concentrate is adsorbed by macroporous resin and eluted with 60 wt% ethanol. The eluent is spray-dried to obtain Rubus idaeus seed extract.

[0051] Example 6

[0052] This embodiment provides an extraction process for extracting Raspberry seeds from Xingan, and the specific extraction process is as follows:

[0053] Take 56 kg of Rubus idaeus seeds, pulverize them using a traditional Chinese medicine pulverizer, and then pass them through an 80-mesh sieve for later use. Take 50 kg of the sieved Rubus idaeus seed powder and add it to an extraction container. Add 8 times the amount of 70 wt% ethanol aqueous solution, and extract three times with ultrasound at 60℃ and normal pressure, 2 hours each time. Combine the extracts, add 3 wt% activated carbon, heat and reflux for decolorization for 1 hour, filter while hot, and concentrate under reduced pressure at 65℃ to obtain Rubus idaeus seed extract concentrate. The concentrate is adsorbed by macroporous resin and eluted with 65 wt% ethanol. The eluent is spray-dried to obtain Rubus idaeus seed extract.

[0054] Example 7

[0055] This embodiment provides an extraction process for gardenia fruit extract, the specific extraction process is as follows:

[0056] Take 55 kg of gardenia fruit, pulverize it using a traditional Chinese medicine pulverizer, and pass it through an 80-mesh sieve to obtain gardenia fruit powder. Take 50 kg of the sieved gardenia fruit powder, add 8 times the amount of 80 wt% ethanol aqueous solution, mix well, and then heat under reflux for extraction twice, 1 hour each time, at an extraction temperature of 50℃. Combine the extracts and concentrate them under reduced pressure at 65℃ to obtain a concentrated gardenia fruit extract. The concentrated extract is adsorbed onto a macroporous resin and eluted with 70 wt% ethanol. The eluent is spray-dried to obtain the gardenia fruit extract.

[0057] Example 8

[0058] This embodiment provides an extraction process for gardenia fruit extract, the specific extraction process is as follows:

[0059] Take 55 kg of gardenia fruit, pulverize it using a traditional Chinese medicine pulverizer, and pass it through an 80-mesh sieve to obtain gardenia fruit powder. Take 50 kg of the sieved gardenia fruit powder, add 10 times the amount of 60 wt% ethanol aqueous solution, mix well, and then heat under reflux for extraction three times, one hour each time, at an extraction temperature of 50℃. Combine the extracts and concentrate them under reduced pressure at 65℃ to obtain a concentrated gardenia fruit extract. The concentrated extract is then adsorbed onto a macroporous resin and eluted with 60 wt% ethanol. The eluent is spray-dried to obtain the gardenia fruit extract.

[0060] Example 9

[0061] This embodiment provides an extraction process for gardenia fruit extract, the specific extraction process is as follows:

[0062] Take 55 kg of gardenia fruit, pulverize it using a traditional Chinese medicine pulverizer, and pass it through an 80-mesh sieve to obtain gardenia fruit powder. Take 50 kg of the sieved gardenia fruit powder, add 9 times the amount of 70 wt% ethanol aqueous solution, mix well, and then heat under reflux for extraction three times, 2 hours each time, at an extraction temperature of 50℃. Combine the extracts and concentrate them under reduced pressure at 65℃ to obtain a concentrated gardenia fruit extract. The concentrated extract is adsorbed onto a macroporous resin and eluted with 65 wt% ethanol. The eluent is spray-dried to obtain the gardenia fruit extract.

[0063] Preparation of plant extract compound antibacterial agent:

[0064] Table 1. Raw material composition of plant extract compound antibacterial agent.

[0065]

[0066] Minimum inhibitory concentration test:

[0067] The test strains: Staphylococcus aureus ATCC6538, Candida albicans ATCC10231 and Aspergillus niger ATCC16404 used in this experiment were all obtained from the Guangdong Provincial Microbial Culture Collection Center.

[0068] Test media: nutrient broth medium and malt extract broth medium.

[0069] Preparation of bacterial suspension:

[0070] (1) Preparation of Staphylococcus aureus bacterial suspension: Under aseptic conditions, add sterile 0.9% sodium chloride aqueous solution to the bacterial slant to wash out a certain amount of bacteria. Then, add the washed bacteria to a sterile container of the same size as the No. 2 turbidity standard tube and dilute with sterile 0.9% sodium chloride aqueous solution until the turbidity is similar to that of the No. 2 turbidity standard tube (approximate bacterial concentration: 6 × 10⁻⁶). 8 The turbidity was the same (cfu / ml).

[0071] (2) Preparation of Candida albicans suspension: Under aseptic conditions, add sterile 0.9% physiological saline to the Candida albicans slant to wash out a certain amount of bacteria. Then, add the washed-out bacteria to a sterile test tube of the same diameter (size) as the turbidity standard tubes No. 3 and No. 4. Finally, add sterile 0.9% physiological saline to dilute until the bacterial suspension contains approximately 10 bacteria. 8 cfu / ml, for later use.

[0072] (3) Preparation of Aspergillus niger suspension: Aspergillus niger spores were eluted with a sterile 0.9% sodium chloride aqueous solution containing 0.05% Tween-80 to prepare an Aspergillus niger spore suspension. The Aspergillus niger spore suspension was filtered to remove mycelia until the bacterial count of the Aspergillus niger spore suspension was approximately 10. 8 cfu / ml, for later use.

[0073] Test operation steps:

[0074] (1) Preparation of culture medium containing antibacterial agent: The plant extract compound antibacterial agent of Examples 10-17 or Comparative Examples 1-3 were prepared into test solutions of different concentrations with distilled water. 2.5 ml of each dilution of test solution was added to a test tube containing 2.5 ml of double concentration nutrient broth.

[0075] (2) Take 0.1 ml containing approximately 10 bacteria. 8 CFU / ml bacterial suspensions were inoculated into test tubes containing nutrient broth containing the plant extract compound antibacterial agent of Examples 10-17 or Comparative Examples 1-3, respectively, as experimental group samples.

[0076] (3) Inoculate test tubes containing the plant extract compound antibacterial agent of Examples 10-17 and Comparative Examples 1-3 in the same way as positive control samples.

[0077] (4) Take two test tubes containing nutritious broth as negative control samples.

[0078] (5) Place the experimental group samples, positive control group samples and negative control group samples in a 37℃ incubator and incubate for 48 hours, and observe the results.

[0079] (6) During the experiment, the bacterial suspension used in the experiment should be cultured and counted for viable bacteria, and its effective concentration should be 5 × 10⁻⁶. 5 cfu / ml ~5×10 6 cfu / ml.

[0080] Evaluation criteria: When the positive control tube shows bacterial growth (turbidity) and the negative control tube shows no bacterial growth (clearness), the effective concentration of the bacterial suspension used in the test is 5 × 10⁻⁶. 5 cfu / ml ~5×10 6 When cfu / ml, the concentration of the plant extract compound antibacterial agent corresponding to the highest dilution at which the experimental group achieved sterile growth is the minimum inhibitory concentration of the sample against the tested bacteria.

[0081] Table 2. Results of determination of minimum inhibitory concentration of plant extract compound antibacterial agent.

[0082]

[0083] As shown in Table 2, there is a significant synergistic antibacterial effect among the extracts of Japanese cypress leaves, raspberry seeds, and gardenia fruit.

[0084] Preservative efficacy test:

[0085] The preservative efficacy test was conducted according to ISO 11930-2019. The Staphylococcus aureus ATCC6538, and the fungi Candida albicans ATCC10231 and Aspergillus niger ATCC16404 used in this experiment were all obtained from the Guangdong Provincial Microbial Culture Collection Center.

[0086] The initial inoculum size was 5.5 × 10⁻⁶. 6 CFU / g or 6.9×10 5 CFU / g was measured on days 7, 14, and 28, and the logarithmic decrease in the number of various microorganisms at the corresponding time points was calculated.

[0087] Preservative efficacy tests of plant extract compound antibacterial agents in eyeshadow and lip gloss: The specific formulas and manufacturing processes of eyeshadow and lip gloss are shown in Tables 3-4.

[0088] Table 3. Eyeshadow Ingredients and Manufacturing Process.

[0089]

[0090] Table 4. Lip Gloss Ingredients and Manufacturing Process.

[0091]

[0092] The experimental results were determined as follows: the number of bacteria decreased by 3 orders of magnitude on day 7, and decreased by 3 orders of magnitude on days 14 and 28 without increasing; the number of yeasts decreased by 1 order of magnitude on day 7, and decreased by 1 order of magnitude on days 14 and 28 without increasing; and the number of Aspergillus did not increase on day 14 and decreased by 1 order of magnitude on day 28. These results were considered as passing the test. See Tables 5 and 6 for the results.

[0093] Table 5. Test data on the preservative efficacy of eyeshadow.

[0094]

[0095] Table 6. Test data on the preservative efficacy of lip gloss.

[0096]

[0097] As shown in Tables 5-6, cosmetics containing the plant extract compound antibacterial agents of Examples 10-17 passed the preservative efficacy test, while cosmetics containing the plant extract compound antibacterial agents of Comparative Examples 1-3 failed the preservative efficacy test. The data clearly show that the examples have a more balanced inhibitory effect on bacteria, yeast, and Aspergillus, so their preservative performance is better and they are more suitable for use in oil and wax-based products to deal with the pollution problems of various microorganisms in the environment.

[0098] Antioxidant capacity test:

[0099] The antioxidant capacity can be reflected by measuring the free radical scavenging ability of 2,2-adiazon-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS). The higher the ABTS free radical scavenging rate, the stronger the antioxidant capacity, and vice versa.

[0100] Refer to GB / T 39100-2020 for the determination of peptide antioxidant activity using the DPPH and ABTS methods. ABTS can be oxidized by K2S2O8 to generate the blue-green free radical cation ABTS. . +, ABTS . The sample exhibits a blue-green color with a maximum absorption peak at 734 nm. As free radicals are scavenged, their numbers decrease, resulting in a lighter color in the solution and consequently a decrease in absorbance at 734 nm. This allows for the calculation of ABTS scavenging in the sample. . + ability.

[0101] The specific steps are as follows:

[0102] Weigh a certain amount of ABTS . +, ABTS was prepared using anhydrous ethanol. . If the concentration is prepared to a level with an absorbance of 0.7 ± 0.02 at 734 nm, then this is ABTS. . +Working solution. The experiment was divided into a solvent background group (T...). a Solvent reaction pore group (T) b ), sample background group (T) c ), sample reaction group (T) d Solvent background group (T) a The solvent is anhydrous ethanol; the solvent reaction pore group (T) b ) is ABTS . +Working solution, solvent; Sample background group (T) c The sample consists of anhydrous ethanol; the sample reaction group (T) d ) is ABTS . +Working solution, sample. Three replicates per group. Add ABTS. . The working solution, prepared solutions of each example and comparative example (1%), anhydrous ethanol, and solvent were added sequentially to a 96-well plate according to the amounts shown in Table 5. The mixture was thoroughly mixed and incubated at 30°C for 10 min. The absorbance was measured at 734 nm using a microplate reader. Vitamin C was used as a positive control. The samples were tested against ABTS. . + The scavenging rate of free radicals is calculated using the following formula:

[0103]

[0104] In formula (1):

[0105] A a — Average absorbance of the solvent background pores;

[0106] A b —Average absorbance of solvent reaction wells;

[0107] A c —Average absorbance of the sample's bottom wells;

[0108] A d —Sample bottom well absorbance.

[0109] Table 7. ABTS Free Radical Scavenging Capacity Sample Table

[0110]

[0111] Table 8. Test results of ABTS free radical scavenging rate.

[0112]

[0113] As shown in Table 8, the antioxidant effect of the combination of Japanese cypress leaf extract, raspberry seed extract, and gardenia fruit extract is better than that of any one of the above components alone, indicating that there is a synergistic antioxidant effect among the three components.

[0114] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that after reading this application specification, they can still modify or make equivalent substitutions to the specific implementation of the present invention, but these modifications or changes do not depart from the protection scope of the pending claims of the present invention.

Claims

1. A plant extract compound antibacterial agent, characterized in that, include: Japanese cypress leaf extract, Rubus idaeus seed extract, Gardenia jasminoides fruit extract.

2. The plant extract compound antibacterial agent according to claim 1, characterized in that, The mass ratio of the Japanese cypress leaf extract, the raspberry seed extract, and the gardenia fruit extract is 1~2:4~7:3~5.

3. The plant extract compound antibacterial agent according to claim 1, characterized in that, The extraction process of the Japanese cypress leaf extract includes the following steps: Japanese cypress leaves were pulverized, and the powder was extracted with ethanol-water solution and ultrasonically extracted. The extract was filtered and concentrated to obtain Japanese cypress leaf extract.

4. The plant extract compound antibacterial agent according to claim 3, characterized in that, The mass ratio of Japanese cypress leaves to ethanol aqueous solution is 1:5~8; And / or, the mass concentration of the ethanol aqueous solution is 50-80%; And / or, the extraction temperature is 50~60℃ and the time is 1~3 hours.

5. The plant extract compound antibacterial agent according to claim 1, characterized in that, The extraction process of the Raspberry Seed Extract includes the following steps: The seeds of Rubus xinganensis were crushed, and the powder was extracted with ethanol-water solution. The extract was purified, filtered, concentrated, and then adsorbed, eluted, and dried through macroporous resin to obtain Rubus xinganensis seed extract.

6. The plant extract compound antibacterial agent according to claim 1, characterized in that, The mass ratio of the seeds of Rubus xinganensis to the ethanol aqueous solution is 1:6~10; And / or, the mass concentration of the ethanol aqueous solution is 60-80%; And / or, the extraction temperature is 50~70℃, and the time is 2~6 hours.

7. The plant extract compound antibacterial agent according to claim 1, characterized in that, The extraction process of the gardenia fruit extract includes the following steps: Gardenia fruit was crushed, and the gardenia fruit powder was extracted by heating and reflux with an ethanol aqueous solution. The extract was concentrated, and the concentrate was adsorbed, eluted, and dried through a macroporous resin to obtain gardenia fruit extract.

8. The plant extract compound antibacterial agent according to claim 7, characterized in that, The mass ratio of gardenia fruit to ethanol aqueous solution is 1:8~10; And / or, the mass concentration of the ethanol aqueous solution is 60-80%; And / or, the extraction temperature is 40~60℃, and the time is 2~6 hours.

9. The application of the plant extract compound antibacterial agent as described in any one of claims 1 to 8 in the preparation of cosmetics, characterized in that, The cosmetic contains the plant extract compound antibacterial agent, and the cosmetic is an oil and wax-based product.

10. The application as described in claim 9, characterized in that, The cosmetics include at least one of foundation cream, lip gloss, eyeshadow, and blush; And / or, by weight percentage, the amount of the plant extract compound antibacterial agent used is 0.5-5% of the cosmetic product.

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