Mirabilitum praeparatum composition with sterilization and mark fading effects as well as preparation method and application of mirabilitum praeparatum composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a mirabilitum praeparatum composition with sterilization and mark fading effects as well as a preparation method and application of the mirabilitum praeparatum composition. According to 100 parts by weight, the mirabilitum praeparatum composition is prepared from the following components in parts by weight: 1.5 to 5 parts of ultra-low molecular weight sodium hyaluronate, 12 to 25 parts of salicylic acid, 6 to 11 parts of Arabic gum, 6 to 11 parts of maltodextrin, 9 to 15 parts of chitosan and the balance of mirabilitum praeparatum. Through scientific proportioning of mirabilitum praeparatum, ultra-low molecular weight sodium hyaluronate, salicylic acid and other components, a synergistic effect of multiple active components is realized, so that the capabilities of inhibiting the activity of propionibacterium acnes, promoting cell migration and inhibiting the expression of inflammatory factor TNF-alpha genes are remarkably improved; therefore, the comprehensive skin care effects of sterilization, acne removal, mark fading and inflammation resistance are achieved, and the problem that the skin care effect of a single component is limited is solved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to a watermelon frost composition with antibacterial and blemish-fading effects, its preparation method and application. Background Technology

[0002] Acne, medically known as "pimples" or "acne vulgaris," and commonly called "zits" or "acne," is a common chronic inflammatory disease of the hair follicles and sebaceous glands. Its pathogenesis involves multiple factors, including excessive sebum secretion, abnormal follicular keratinization, proliferation of Propionibacterium acnes, and inflammatory response. Currently, most acne treatment products on the market rely on chemically synthesized ingredients such as salicylic acid and benzoyl peroxide, or plant extracts. However, they generally suffer from the following problems: 1) Single ingredients cannot simultaneously achieve a balance between highly effective sterilization and gentle repair. For example, although salicylic acid can dissolve keratin and inhibit bacteria, high concentrations can easily cause dry skin and stinging; 2) Traditional plant extracts (such as tea tree oil and honeysuckle) have anti-inflammatory effects, but their penetration is insufficient, making it difficult to reach the root of the hair follicle to exert their effects; 3) Existing products have limited effects on repairing post-inflammatory hyperpigmentation (acne scars) and lack effective ingredients that promote collagen regeneration in the dermis.

[0003] Therefore, providing an active ingredient that is effective in treating acne and has minimal side effects on the skin has become an urgent problem to be solved. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a watermelon frost composition with bactericidal and stain-fading effects, its preparation method and application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention includes: In a first aspect, the present invention provides a watermelon frost composition with bactericidal and stain-fading effects. The watermelon frost composition comprises the following components in parts by weight: 1.5-5 parts of ultra-low molecular weight sodium hyaluronate, 12-25 parts of salicylic acid, 6-11 parts of gum arabic, 6-11 parts of maltodextrin, 9-15 parts of deacetylated chitosan, and the balance being watermelon frost.

[0006] Watermelon frost, as the core active ingredient of the composition described in this invention, possesses natural antibacterial and anti-inflammatory properties. It can inhibit the proliferation of harmful microorganisms on the skin surface, alleviate inflammatory responses, and provide a foundation for sterilization and fading of acne scars. Simultaneously, its mild composition reduces the irritation of other functional components and is compatible with the skin's physiological environment. Ultra-low molecular weight sodium hyaluronate, with its small molecular weight and strong skin penetration, can quickly replenish moisture in the stratum corneum, improve dry and rough skin, create a good skin foundation for fading acne scars, and promote skin metabolism, accelerate the repair of damaged tissue, and help reduce pigmentation of acne scars. Salicylic acid, as a lipid-soluble component, can penetrate deep into pores to dissolve oil, unclog hair follicles, and exert targeted acne-removing effects. It can also gently exfoliate dead skin cells, accelerating the fading of acne scars. Experimental studies have shown that the three active ingredients described above can form a synergistic effect when combined within the preferred ratio range of this invention, thereby significantly enhancing the antibacterial effect of the composition against Propionibacterium acnes, effectively promoting cell migration, inhibiting the expression of the inflammatory factor TNF-α gene, and achieving multiple effects such as excellent antibacterial and acne-removing properties, fading of acne marks, and soothing and anti-inflammatory effects.

[0007] Meanwhile, the watermelon frost composition of this invention employs microencapsulation technology to construct its core system, using gum arabic, maltodextrin, and deacetylated chitosan as microcapsule wall materials to encapsulate three active substances: watermelon frost, ultra-low molecular weight sodium hyaluronate, and salicylic acid. This technology utilizes the sustained-release effect of the wall materials to achieve slow penetration of the active ingredients into the skin, ensuring the sustainability of skincare efficacy. Simultaneously, salicylic acid can couple with gum arabic molecules, significantly optimizing its skin permeability and enabling precise targeting of salicylic acid to the stratum corneum, reducing skin irritation at its source. Furthermore, deacetylated chitosan possesses broad-spectrum antibacterial activity, synergistically interacting with watermelon frost and salicylic acid to enhance bactericidal effects and help fade skin marks, thereby comprehensively improving the overall skincare efficacy of the composition.

[0008] Preferably, the watermelon frost composition comprises, by weight, the following components in 100 parts: 2.3-4.2 parts of ultra-low molecular weight sodium hyaluronate, 13.7-20 parts of salicylic acid, 8.71-9 parts of gum arabic, 8.71-10 parts of maltodextrin, 11-12.58 parts of deacetylated chitosan, and the balance being watermelon frost.

[0009] Preferably, the watermelon frost composition comprises, in 100 parts by weight, the following components: 3.23 parts of ultra-low molecular weight sodium hyaluronate, 18.39 parts of salicylic acid, 8.71 parts of gum arabic, 8.71 parts of maltodextrin, 12.58 parts of deacetylated chitosan, and the balance being watermelon frost.

[0010] Experimental studies have shown that when the active components in the composition are combined in the optimal ratio specified above, the prepared composition can achieve the best antibacterial effect against Propionibacterium acnes, while significantly enhancing cell migration ability and effectively inhibiting the expression of inflammatory factors. Thus, it works synergistically from multiple dimensions such as sterilization, acne removal, fading of marks and anti-inflammation, maximizing the comprehensive efficacy of the composition.

[0011] Preferably, the weight-average molecular weight of the ultra-low molecular weight sodium hyaluronate is 600-1000 Da.

[0012] In this invention, the molecular weight of sodium hyaluronate is a key factor affecting its skin penetration effect. This invention preferably uses sodium hyaluronate with a weight-average molecular weight of 600-1000 Da. Sodium hyaluronate with this molecular weight can quickly penetrate the skin's stratum corneum barrier, reaching the dermis and the site of scar formation. This effectively overcomes the shortcomings of high molecular weight sodium hyaluronate, which tends to remain on the skin surface and has difficulty exerting deep effects. It provides a carrier for the targeted delivery of active ingredients (such as salicylic acid and watermelon frost extract), and forms a synergistic effect with these active ingredients, further enhancing the bactericidal efficacy and scar-fading effect of the composition.

[0013] Secondly, the present invention provides the application of the watermelon frost composition with antibacterial and mark-fading effects in the preparation of cosmetics.

[0014] Preferably, the watermelon frost composition with antibacterial and mark-fading effects has a mass percentage of 1-15% in the cosmetic.

[0015] Thirdly, the present invention provides a method for preparing the watermelon frost composition, comprising the following steps: S1. Mix watermelon frost, salicylic acid, ultra-low molecular weight sodium hyaluronate, gum arabic, maltodextrin and deacetylated chitosan to obtain a mixture; S2. The mixture is dried and ground to obtain the watermelon frost composition.

[0016] Preferably, in step S1, the mixing method includes high-speed shear dispersion or high-pressure homogenization; the high-speed shear dispersion conditions are 15000-25000 rpm, and the time is 5-12 min; the pressure of the high-pressure homogenization is 800-1500 bar, and the number of cycles is 3-5.

[0017] Preferably, in step S2, the drying method includes spray drying; the conditions for spray drying are: inlet air temperature of 100-120℃; outlet air temperature of 50-70℃.

[0018] Fourthly, the present invention provides an essence with antibacterial and fading effects on marks, the essence comprising the watermelon frost composition described above.

[0019] Preferably, the essence comprises the following components in weight percentages: 2-10% watermelon frost composition, 0.3-0.8% p-hydroxyacetophenone, 0.2-1.5% propylene glycol, 3-7% PEG-400, 0.1-0.5% SEPIMAX ZEN, 0.08-0.35% Silwax® WS, 0.05-0.2% allantoin, 0.15-0.4% xanthan gum, 0.2-1.5% hexanediol, 1-5% acne-reducing agent, 0.5-2% skin beautifying agent, and the balance being water.

[0020] More preferably, the essence comprises the following components in weight percentages: 6.2% watermelon frost composition, 0.5% p-hydroxyacetophenone, 0.5% propylene glycol, 5% PEG-400, 0.3% SEPIMAX ZEN, 0.15% Silwax® WS, 0.1% allantoin, 0.25% xanthan gum, 0.5% hexanediol, 3% acne-reducing agent, 1% skin-beautifying agent, and the balance being water.

[0021] Fifthly, the present invention provides a method for preparing the aforementioned essence, comprising the following steps: S1. Mix water, p-hydroxyacetophenone, propylene glycol, PEG-400, SEPIMAX ZEN, Silwax® WS, allantoin and xanthan gum, heat to 80-90℃, homogenize, and keep warm to obtain mixture A; S2. Cool mixture A to 30-50℃, add watermelon frost composition, hexanediol, acne treatment and beauty essence, mix well to obtain the essence.

[0022] Preferably, in step S1, the homogenization pressure is 1000-2000 bar, the homogenization cycle is 2-4 times, and the heat preservation time is 15-30 min.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) This invention achieves the synergistic effect of multiple active ingredients through the scientific ratio of watermelon frost, ultra-low molecular weight sodium hyaluronate, salicylic acid and other components, thereby significantly enhancing the ability to inhibit the activity of Propionibacterium acnes, promote cell migration and inhibit the expression of inflammatory factor TNF-α gene, and macroscopically achieves comprehensive skin care effects of sterilization, acne removal, fading marks and anti-inflammation, solving the problem of limited skin care effects of single ingredients; (2) The present invention uses a microcapsule encapsulation technology with gum arabic, maltodextrin and deacetylated chitosan as wall materials to encapsulate the core active substances. Through the sustained release mechanism, the active ingredients can slowly and continuously penetrate into the skin, prolong the skin care effect time and avoid the effect decay caused by rapid release of active ingredients. At the same time, the microcapsule structure can protect the stability of active ingredients, reduce the impact of the external environment on its efficacy, and improve the stability of the composition during storage and use. (3) The selection and proportion of each component in this invention take into account both efficacy and gentleness. By combining microcapsule encapsulation technology and molecular coupling optimization, while ensuring the effective skin care effect, the adverse effects of irritating ingredients on the skin are reduced. It is suitable for a variety of skin types, especially meeting the gentle care needs of acne-prone and other problematic skin types, and improving the safety and applicability of the product. Attached Figure Description

[0024] Figure 1 Comparison of the volunteers' facial condition before and after using the serum in Example 2; Figure 2 The image shows the finished product of the serum used in Example 2. Detailed Implementation

[0025] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0026] The watermelon frost described in this invention is sourced from Guangzhou Lanxi Cosmetics Co., Ltd. The acne treatment, product number OTL-043, is sourced from Guangzhou Lanxi Cosmetics Co., Ltd. The skin-beautifying essence, product number OTL-1152, is sourced from Guangzhou Lanxi Cosmetics Co., Ltd. The SEPIMAX ZEN is sourced from SEPPIC. The deacetylated chitosan is sourced from Guangzhou Lanxi Cosmetics Co., Ltd. The Silwax® WS product, model number Silwax WS Special, is sourced from Siltech Corporation. Unless otherwise specified, all other ingredients are commercially available generic ingredients.

[0027] Examples 1-6 Examples 1-6 provide a watermelon frost composition with antibacterial and mark-fading effects. The formulation of the composition is shown in Table 1 (total weight parts: 100 parts).

[0028] Table 1. Formulation table (parts by weight) of the watermelon frost compositions described in Examples 1-6 Among them, the weight-average molecular weight of the ultra-low molecular weight sodium hyaluronate in Example 1 was 600±30 Da; the weight-average molecular weight of the ultra-low molecular weight sodium hyaluronate in Examples 2-5 was 800±30 Da; and the weight-average molecular weight of the ultra-low molecular weight sodium hyaluronate in Example 6 was 1000±30 Da.

[0029] The preparation method of the watermelon frost composition with antibacterial and mark-fading effects in Example 1 includes the following steps: S1. Watermelon frost, salicylic acid, ultra-low molecular weight sodium hyaluronate, gum arabic, maltodextrin and deacetylated chitosan were dispersed and mixed by high-speed shearing (20,000 rpm, 8 min) to obtain a mixture; S2. The mixture is spray-dried (inlet air temperature 120℃, outlet air temperature 70℃) and ground to obtain a watermelon frost composition with antibacterial and mark-fading effects.

[0030] The preparation method of the watermelon frost composition with antibacterial and mark-fading effects in Examples 2-5 includes the following steps: S1. Watermelon frost, salicylic acid, ultra-low molecular weight sodium hyaluronate, gum arabic, maltodextrin and deacetylated chitosan were mixed by high-pressure homogenization (pressure 1200 bar, cycle 4 times) to obtain a mixture. S2. The mixture is spray-dried (inlet air temperature 110℃, outlet air temperature 60℃) and ground to obtain a watermelon frost composition with antibacterial and mark-fading effects.

[0031] The preparation method of the watermelon frost composition with antibacterial and mark-fading effects in Example 6 includes the following steps: S1. Watermelon frost, salicylic acid, ultra-low molecular weight sodium hyaluronate, gum arabic, maltodextrin and deacetylated chitosan were mixed by high-pressure homogenization (pressure 1500 bar, cycle 3 times) to obtain a mixture. S2. The mixture is spray-dried (inlet air temperature 100℃, outlet air temperature 50℃) and ground to obtain a watermelon frost composition with antibacterial and mark-fading effects.

[0032] Comparative Examples 1-9 Comparative Examples 1-9 provide a composition, the formulation of which is shown in Table 2 (total parts by weight 100 parts). The preparation method of the composition is the same as that of the Examples, except for the formulation.

[0033] Table 2 Formulation table (parts by weight) of the compositions described in Comparative Examples 1-9 The preparation methods of the compositions in Comparative Examples 1-9 are the same as those in Example 2.

[0034] Comparative Example 10 This comparative example provides a composition that differs from Example 2 only in that an equal amount of sodium hyaluronate with a weight-average molecular weight of 5 kDa is used to replace the ultra-low molecular weight sodium hyaluronate. All other components, amounts, and preparation methods are the same as in Example 2.

[0035] Comparative Example 11 This comparative example provides a composition that differs from Example 2 only in that an equal amount of ethylene glycol salicylate is used to replace salicylic acid, while the remaining components, amounts, and preparation methods are the same as in Example 2.

[0036] Comparative Example 12 This comparative example provides a composition that differs from Example 2 only in that an equal amount of witch hazel extract is used to replace watermelon frost, while the remaining components, amounts, and preparation methods are the same as in Example 2.

[0037] Comparative Example 13 This comparative example provides a composition that differs from Example 2 only in that an equal amount of carboxymethyl deacetylated chitosan is used to replace deacetylated chitosan, while the remaining components, amounts, and preparation methods are the same as in Example 2.

[0038] Comparative Example 14 This comparative example provides a composition that differs from Example 2 only in that an equal amount of β-glucan is used to replace maltodextrin, while the remaining components, amounts, and preparation methods are the same as in Example 2.

[0039] Comparative Example 15 This comparative example provides a composition that differs from Example 2 only in that an equal amount of xanthan gum is used instead of gum arabic, while the other components, amounts, and preparation methods are the same as in Example 2.

[0040] Application Examples 1-4 Application Examples 1-4 provide an essence with antibacterial and fading effects on marks. The watermelon frost composition in the essence corresponds to the watermelon frost composition described in Examples 1-3 and 6, respectively. The specific formula of the essence is shown in Table 3.

[0041] Table 3 shows the formula (percentage by mass) of the serums described in Application Examples 1-4. The preparation method of the serum with antibacterial and blemish-fading effects in Application Example 1 includes the following steps: (1) Mix water, p-hydroxyacetophenone, propylene glycol, PEG-400, SEPIMAX ZEN, Silwax® WS, allantoin and xanthan gum, heat to 90°C and then homogenize at 1000 bar 4 times, and keep at 90°C for 15 min to obtain mixture A; (2) Cool down to 50°C, add watermelon frost composition, hexanediol, acne treatment and beauty essence to mixture A, mix evenly to obtain an essence with antibacterial and fading effects.

[0042] The preparation method of the serum with antibacterial and blemish-fading effects used in Examples 2 and 3 includes the following steps: (1) Mix water, p-hydroxyacetophenone, propylene glycol, PEG-400, SEPIMAX ZEN, Silwax® WS, allantoin and xanthan gum, heat to 85°C and then homogenize at 1500 bar three times, and keep at 85°C for 20 min to obtain mixture A; (2) Cool down to 40°C, add watermelon frost composition, hexanediol, acne treatment and beauty essence to mixture A, mix evenly to obtain an essence with antibacterial and fading effects.

[0043] The preparation method of the serum with antibacterial and blemish-fading effects in Application Example 4 includes the following steps: (1) Mix water, p-hydroxyacetophenone, propylene glycol, PEG-400, SEPIMAX ZEN, Silwax® WS, allantoin and xanthan gum, heat to 80°C and then homogenize twice at 2000 bar, and keep warm at 80°C for 30 min to obtain mixture A; (2) Cool down to 30°C, add watermelon frost composition, hexanediol, acne treatment and beauty essence to mixture A, mix evenly to obtain an essence with antibacterial and fading effects.

[0044] Test Example 1 This test example uses the compositions described in Examples 1-6 and Comparative Examples 1-15 as test samples to test their inhibitory effect on Propionibacterium acnes, repair effect, and anti-inflammatory effect. The specific methods are as follows: (1) Antibacterial test Test strain: Propionibacterium acnes ATCC 11827.

[0045] Test method: Refer to the Ministry of Health's 2002 edition of "Disinfection Technical Specifications" - 2.1.8.3 Minimum inhibitory concentration test (agar dilution method). Specific test results are shown in Table 4.

[0046] Table 4 (2) Repair efficacy test Test Principle: Cell migration, also known as cell crawling, cell movement, or cell motion, refers to the movement of cells after receiving migration signals or sensing gradients of certain substances. Cell migration is a spatiotemporal alternating process of cell head pseudopodia extension, new adhesion establishment, and cell body tail contraction. Cell migration ability is also an important indicator in skin repair. This experiment uses L929 fibroblasts as an in vitro model to detect the effect of samples on the migration ability of fibroblasts and to evaluate whether the samples have the effect of promoting skin repair.

[0047] Test samples: The compositions of Examples 1-6 and Comparative Examples 1-15 were prepared into 1% solutions by mass using H-DMEM medium containing 10% FBS.

[0048] Test procedure: Take L929 cells in the logarithmic growth phase and add 2×10⁻⁶ cells to each well of a 6-well culture plate. 5 Cells were cultured in H-DMEM medium containing 10% FBS (hereinafter referred to as culture medium) for 24 h at 37℃, 5% CO2, and saturated humidity. The culture medium was then aspirated, and cross-shaped lines were drawn using a 1 mL pipette tip. The negative control group was added to the culture medium, and each sample group was added to the corresponding test sample. Culture was continued for another 24 h. After culture, cells were fixed with paraformaldehyde, stained with crystal violet, and photographed under a microscope. Cell migration at the scratched areas of the negative control group and each test sample group was observed under a microscope at 0 h and 24 h, and images of the scratched areas were taken. The scratch area of ​​each group was measured using ImageJ software.

[0049] Cell migration rate (%) = (1-S) 24 / S0)×100% In the formula: S 24 S0 represents the area of ​​the scratched area after 24 hours; S0 represents the area of ​​the scratched area when it is 0.

[0050] The test results are shown in Table 5.

[0051] Table 5 (3) Anti-inflammatory efficacy test Test Principle: Inflammation is one of the most common clinical conditions, a defensive response of the body to remove harmful stimuli and repair damaged tissues. When human immune cells are exposed to inflammatory factors, some small-molecule soluble proteins or peptides with specific immunomodulatory functions, capable of intercellular communication, are secreted by the body and participate in or cause inflammatory responses. These substances are called inflammatory factors, including NO, TNF-α, IL-6, PGE-2, IL-1, and TGF-β1. TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by immune cells such as macrophages and monocytes, playing a central role in inflammatory responses, immune regulation, and apoptosis. Acne is a chronic inflammatory disease of the pilosebaceous unit, and TNF-α plays a crucial role in its pathogenesis: on the one hand, the metabolites of Propionibacterium acnes activate keratinocytes and macrophages, releasing TNF-α and triggering an inflammatory response in the hair follicle wall. On the other hand, TNF-α promotes keratinocyte proliferation and parakeratosis, leading to follicle blockage and the formation of microcomedones. Therefore, inhibiting the expression of the inflammatory factor TNF-α not only demonstrates the anti-inflammatory effect of the sample, but also indicates that the sample exhibits acne-removing potential to some extent.

[0052] Test samples: The compositions of Examples 1-6 and Comparative Examples 1-15 were prepared into solutions with a mass concentration of 0.5% using H-DMEM medium containing 10% FBS.

[0053] Test procedure: Take L929 cells in the logarithmic growth phase and add 2×10⁻⁶ cells to each well of a 6-well culture plate. 5 Cells were added to H-DMEM medium (hereinafter referred to as culture medium) containing 10% FBS and incubated at 37°C with 5% CO2. 2 After culturing for 24 hours under saturated humidity, the culture medium was discarded. The model group and test sample group were stimulated with 1 mL of culture medium containing LPS, while the negative control group was stimulated with culture medium. The mixtures were then incubated at 37℃ and 5% CO2. 2 Cells were cultured for 6 hours under saturated humidity, and the culture medium was discarded. Test samples were added to the test sample group, while culture medium was added to the model group and negative control group. After 24 hours of culture, cells from each group were lysed to collect RNA, which was reverse transcribed into cDNA and stored at -80℃. Finally, quantitative real-time PCR was performed to determine the expression level of TNF-α. The gene expression level was calculated relative to the negative control group (100%).

[0054] The test results are shown in Table 6.

[0055] Table 6 Propionibacterium acnes can decompose triglycerides in sebum, producing free fatty acids that stimulate hair follicles and surrounding tissues, triggering inflammatory responses and leading to acne symptoms such as redness, swelling, and pustules. The test results in Tables 4-6 above show that the watermelon frost composition of this invention can effectively inhibit the growth of Propionibacterium acnes and significantly inhibit the expression of the inflammatory factor TNF-α, thereby alleviating skin redness and inflammation and achieving excellent acne-removing effects. Furthermore, the watermelon frost composition of this invention can also promote cell migration, thereby accelerating wound healing in acne-affected areas and demonstrating the potential to fade acne scars. All the above results collectively confirm that the watermelon frost composition of this invention possesses excellent efficacy in antibacterial, acne-removing, and scar-fading effects.

[0056] In Comparative Examples 1-3, any one of the three active components—ultra-low molecular weight sodium hyaluronate, salicylic acid, and watermelon frost—was omitted, or in Comparative Examples 11-12, the active components were replaced. Even though the total amount of the three active components remained the same as in Example 2, their antibacterial (inhibiting Propionibacterium acnes), repair (promoting cell migration), and anti-inflammatory (inhibiting the expression of the inflammatory factor TNF-α) effects were significantly lower than in the Example. This indicates that ultra-low molecular weight sodium hyaluronate, salicylic acid, and watermelon frost, as core active components, have an irreplaceable synergistic effect. The omission or replacement of any component will disrupt the overall efficacy system, leading to a significant decrease in efficacy. In Comparative Examples 8-9, when the proportion of the three core active components deviated from the preferred range of this invention, the antibacterial, repair, and anti-inflammatory effects of the composition also decreased significantly compared to the Example. This result confirms that precise control of the proportion of each component in the composition described in this invention is a key factor in ensuring the optimal efficacy of the composition, and an imbalance in the ratio will directly affect the expression of efficacy.

[0057] In Comparative Example 10, when conventional high molecular weight sodium hyaluronate was used to replace the preferred hyaluronate with a weight-average molecular weight of 600-1000 Da, the overall efficacy of the composition, including antibacterial and anti-inflammatory effects, was still reduced to some extent compared to the example. This indicates that the preferred ultra-low molecular weight (600-1000 Da) of the present invention is a key structural feature for sodium hyaluronate to exert its efficacy in the composition. Its molecular weight directly affects the bioactivity and efficacy of the component, and conventional high molecular weight sodium hyaluronate cannot achieve the same level of efficacy.

[0058] In Comparative Examples 4-7, the efficacy of the compositions prepared by omitting any one or two of the three auxiliary components—deacetylated chitosan, gum arabic, and maltodextrin—or by replacing these three auxiliary components with other components of similar properties as in Comparative Examples 13-15, was lower than that of the examples. Analysis of the component characteristics shows that deacetylated chitosan, gum arabic, and maltodextrin, as microcapsule wall materials in the compositions, not only undertake the core functions of sustained release and enhanced penetration, but also synergistically enhance the overall efficacy of the compositions. Therefore, whether some wall material components are omitted or other components are replaced, the sustained-release and enhanced-penetration structure of the microcapsules will be disrupted, causing the active components to fail to function effectively, ultimately resulting in a decrease in the antibacterial, repairing, and anti-inflammatory effects of the compositions.

[0059] Test Example 2 This test example uses the serums described in Application Examples 1-4 as test samples to test their acne-removing efficacy, and compares the efficacy using a base serum. The preparation method of the base serum is the same as in Application Example 2, except that the watermelon frost composition is replaced with an equal amount of deionized water. The specific test method is as follows: Fifty healthy volunteers aged 18-45 years with oily skin and long-term acne with acne scars were randomly divided into 5 groups of 10 each. Each group used one serum. After receiving the test samples, volunteers used the serums according to their personal habits and were not allowed to use other products claiming to have acne-reducing effects. Volunteers rated their experience and effectiveness at the beginning of use and after 4 weeks of use. The rating criteria ranged from no effect to significant effect. No effect was 0 points, and significant effect was 10 points, with scores calculated based on the degree of effect. The average score of the 10 volunteers was then tallied. The rating results are shown in Table 7.

[0060] Table 7 The above human efficacy test results show that, compared with the base essence, the essence containing the watermelon composition of the present invention has a better effect on reducing redness, removing acne and fading marks, and the effect of application Example 2 (the watermelon frost composition of Example 2) is the best.

[0061] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A watermelon frost composition with antibacterial and mark-fading effects, characterized in that, Based on 100 parts by weight, the watermelon frost composition comprises the following components in parts by weight: 1.5-5 parts of ultra-low molecular weight sodium hyaluronate, 12-25 parts of salicylic acid, 6-11 parts of gum arabic, 6-11 parts of maltodextrin, 9-15 parts of deacetylated chitosan, and the balance being watermelon frost.

2. The watermelon frost composition as described in claim 1, characterized in that, Based on 100 parts by weight, the watermelon frost composition comprises the following components in parts by weight: 2.3-4.2 parts of ultra-low molecular weight sodium hyaluronate, 13.7-20 parts of salicylic acid, 8.71-9 parts of gum arabic, 8.71-10 parts of maltodextrin, 11-12.58 parts of deacetylated chitosan, and the balance being watermelon frost.

3. The watermelon frost composition as described in claim 2, characterized in that, Based on 100 parts by weight, the watermelon frost composition comprises the following components in parts by weight: 3.23 parts of ultra-low molecular weight sodium hyaluronate, 18.39 parts of salicylic acid, 8.71 parts of gum arabic, 8.71 parts of maltodextrin, 12.58 parts of deacetylated chitosan, and the balance being watermelon frost.

4. The watermelon frost composition as described in claim 1, characterized in that, The ultra-low molecular weight sodium hyaluronate has a weight-average molecular weight of 600-1000 Da.

5. The use of the watermelon frost composition with bactericidal and fading effects as described in any one of claims 1-4 in the preparation of cosmetics.

6. The application as described in claim 5, characterized in that, The watermelon frost composition with antibacterial and fading effects has a mass percentage of 1-15% in cosmetics.

7. The method for preparing the watermelon frost composition according to any one of claims 1-4, characterized in that, Includes the following steps: S1. Mix watermelon frost, salicylic acid, ultra-low molecular weight sodium hyaluronate, gum arabic, maltodextrin and deacetylated chitosan to obtain a mixture; S2. The mixture is dried and ground to obtain a watermelon frost composition with antibacterial and stain-fading effects.

8. An essence with antibacterial and blemish-fading effects, characterized in that, The essence comprises the watermelon frost composition with antibacterial and fading effects as described in any one of claims 1-4.

9. The essence as described in claim 8, characterized in that, The essence comprises the following components in weight percentages: 2-10% watermelon frost composition, 0.3-0.8% p-hydroxyacetophenone, 0.2-1.5% propylene glycol, 3-7% PEG-400, 0.1-0.5% SEPIMAXZEN, 0.08-0.35% Silwax®WS, 0.05-0.2% allantoin, 0.15-0.4% xanthan gum, 0.2-1.5% hexanediol, 1-5% acne-reducing agent, 0.5-2% skin beautifying agent, and the balance being water.

10. The method for preparing the essence as described in claim 9, characterized in that, Includes the following steps: S1. Mix water, p-hydroxyacetophenone, propylene glycol, PEG-400, SEPIMAX ZEN, Silwax® WS, allantoin and xanthan gum, heat to 80-90℃, homogenize, and keep warm to obtain mixture A; S2. Cool mixture A to 30-50℃, add watermelon frost composition, hexanediol, acne treatment and beauty essence, mix well to obtain the essence.

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