Soothing and anti-allergy plant extract composition and cleansing cream

Abstract

The invention discloses a soothing and anti-allergy plant extract composition and a cleansing cream. The soothing and anti-allergy plant extract composition comprises a chamomile extract, a poria cocos extract and a purslane extract, the mass ratio of the chamomile extract to the poria cocos extract to the purslane extract in the composition is 100: (10-30): (30-50). The soothing and anti-allergy plant extract composition takes the chamomile extract, the poria cocos extract and the purslane extract as raw materials, and the three extracts are excellent in compatibility and can generate a synergistic effect in the aspects of skin soothing and anti-allergy after being compounded according to a proper ratio. On the basis, the soothing and anti-allergy plant extract composition is loaded in bran and then applied to the cleansing cream, the activity stability of the soothing and anti-allergy plant extract in a system can be improved, meanwhile, the physical massage exfoliating effect of the bran is matched, transdermal absorption of active ingredients can be promoted, and then the soothing and anti-allergy effects of the product are improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to a soothing and anti-allergic plant extract composition and a facial cleanser. Background Technology

[0002] In recent years, with the deepening of research on the functionality of plant extracts and the general advantages of plant extracts such as being natural, environmentally friendly, mild, and low in irritation, the application of natural plant extracts in cosmetics, detergents, biomedicine, food, and other fields has become increasingly widespread. In particular, in the cosmetics field, consumers prefer products containing ingredients derived from natural plant extracts.

[0003] Chamomile is an herbaceous plant native to Europe, and also cultivated in Xinjiang and other regions of my country. Chamomile extract contains abundant flavonoids, polyphenols, and other active ingredients, exhibiting various biological activities such as lowering blood sugar and lipids, anti-oxidation, and anti-inflammation. It has been used in various foods and cosmetics. Poria cocos, the dried sclerotium of the fungus *Poria cocos*, is a commonly used traditional Chinese medicine. Its chemical components mainly include triterpenoids, polysaccharides, terpenes, sterols, proteins, organic acids, and flavonoids, possessing pharmacological effects such as anti-tumor, anti-oxidation, anti-aging, and immunomodulatory properties. Polysaccharides and terpenes are its main active ingredients. Purslane is also a traditional Chinese medicinal plant, mainly containing alkaloids, terpenes, coumarins, flavonoids, and organic acids. It possesses antibacterial, anti-inflammatory, anti-oxidative, anti-aging, and anti-allergic pharmacological activities and is currently used in some cosmetics.

[0004] In modern society, the pressures of work and study, coupled with a fast-paced lifestyle, generally weaken people's resistance, leading to more fragile and sensitive skin. Prolonged exposure to ultraviolet radiation or contact with various allergens increases the likelihood of various skin allergies, resulting in a significant market demand for soothing and anti-allergy products. Our team discovered in preliminary experiments that a combination of chamomile extract, poria cocos extract, and purslane extract exhibits superior synergistic effects in soothing and anti-allergy compared to individual extracts of the same ingredient. Currently, there are no reports or products on the soothing and anti-allergy effects of this combination. Therefore, developing a skin cleansing product, such as a facial cleanser based on natural plant active ingredients, with soothing and anti-allergy effects has considerable market potential.

[0005] However, in subsequent experiments, the invention team discovered that due to the complex composition of the ingredients in products such as facial cleansers, the bioactivity of chamomile extract, poria cocos extract, and purslane extract is easily affected by other ingredients, thus failing to fully exert their soothing and anti-allergic effects. Therefore, how to successfully formulate a facial cleanser product with stable soothing and anti-allergic effects by combining chamomile extract, poria cocos extract, and purslane extract is a technical challenge that must be solved in the process of commercializing this product.

[0006] Furthermore, the invention team also discovered that when chamomile extract, poria cocos extract, and purslane extract are directly added to products such as facial cleansers, the short contact time makes it difficult for these extracts to quickly penetrate the skin due to the barrier of the stratum corneum, resulting in less than ideal soothing and anti-allergic effects. Therefore, improving the transdermal absorption of chamomile extract, poria cocos extract, and purslane extract in rinse-off products is a technical problem that needs to be solved. Summary of the Invention

[0007] This invention provides a soothing and anti-allergic plant extract composition and a facial cleanser. First, the soothing and anti-allergic plant extract composition of this invention uses chamomile extract, poria cocos extract, and purslane extract as raw materials. This invention has discovered that these three extracts have excellent compatibility, and when combined in appropriate proportions, they can produce a synergistic effect in soothing and allergic skin. Second, this invention loads the soothing and anti-allergic plant extract composition onto wheat bran and then applies it to the facial cleanser. This improves the activity and stability of the soothing and anti-allergic plant extract in the system. Simultaneously, combined with the physical massage and exfoliating effect of wheat bran, it promotes the transdermal absorption of active ingredients, thereby enhancing the product's soothing and anti-allergic efficacy.

[0008] The specific technical solution of the present invention includes: First, the present invention provides a soothing and anti-allergic plant extract composition comprising chamomile extract, poria cocos extract and purslane extract; wherein the mass ratio of chamomile extract, poria cocos extract and purslane extract in the composition is 100:(10-30):(30-50).

[0009] This invention relates to a soothing and anti-allergic plant extract composition using chamomile extract, poria cocos extract, and purslane extract as active ingredients. Through experiments, this invention has found that these three extracts have excellent compatibility; when combined in appropriate proportions, they produce a synergistic effect of "1+1+1>3" in soothing and anti-allergic effects on the skin. Replacing some of these extracts with other substances with similar effects does not achieve a similar level of synergistic effect. Through research and analysis, the team of this invention has discovered that their synergistic mechanism may be as follows: First, compared to single-pathway effects, the three components in the composition of this invention achieve their soothing and anti-allergic effects through different pathways, resulting in better efficacy. Specifically, the flavonoids in purslane significantly inhibit trypsin secretion and mast cell degranulation, thus exerting a soothing effect; chamomile is rich in polyphenols, which can reduce ROS levels and downregulate T cell activation, migration, and differentiation, thereby exerting a soothing and anti-allergic effect; Poria cocos contains a large amount of polysaccharides, which can significantly inhibit cyclooxygenase (COX) activity and suppress the release of inflammatory factors such as prostaglandins and leukotrienes; simultaneously, polysaccharides also have significant moisturizing and barrier repair effects on the skin.

[0010] Secondly, this invention discovers that purslane, chamomile, and poria cocos not only enhance their soothing and anti-allergic effects through multiple pathways, but more importantly, they also have a synergistic effect while exerting their individual effects. Specifically, purslane extract, while inhibiting mast cell degranulation to reduce skin sensitivity, can also significantly enhance the effect of poria cocos polysaccharides in downregulating the expression of pro-inflammatory factors and reducing inflammatory responses; simultaneously, poria cocos extract, while reducing the release of inflammatory factors, can also enhance the function of chamomile extract in scavenging ROS and reducing the body's oxidative stress response. In summary, this invention, by combining purslane, chamomile, and poria cocos, can achieve long-lasting and significant anti-inflammatory and soothing effects.

[0011] Preferably, the Poria cocos extract and Portulaca oleracea extract are water extracts or alcohol-water extracts.

[0012] Secondly, the present invention provides a soothing and anti-allergic facial cleanser containing a composition of soothing and anti-allergic plant extracts, comprising the following raw materials in weight percentages: surfactant 40-6%, moisturizer 3-10%, soothing and anti-allergic bran particles 0.1-1.5%, thickener 0.1-0.3%, preservative 0-1%, pH adjuster 0-0.5%, and solvent.

[0013] The soothing and anti-allergic bran granules include a bran granule carrier that has undergone enzymatic hydrolysis and cooking, and chamomile extract, poria cocos extract and purslane extract loaded in the bran granule carrier.

[0014] As described in the background section, on the one hand, this invention has found that due to the complex composition of the ingredients in products such as facial cleansers, the bioactivity of chamomile extract, poria cocos extract, and purslane extract is easily affected by other ingredients, thus failing to fully exert their soothing and anti-allergic effects. On the other hand, we have found that when chamomile extract, poria cocos extract, and purslane extract are directly added to products such as facial cleansers, the short contact time makes it difficult for the active ingredients in the extracts to quickly penetrate into the skin due to the barrier of the stratum corneum, resulting in a less than ideal soothing and anti-allergic effect.

[0015] Therefore, this invention has successfully solved the above two technical problems through subsequent improvements. Specifically, this invention adds bran particles to the formula. In the prior art, there are related reports (e.g., CN102321715A, CN105213287A, etc.) that bran has a physical massage effect and can remove aged keratin during use, thus it can be applied to various skin cleansing products. Unlike the prior art, in this invention, bran also serves as a carrier for the extract. On the one hand, after pretreatment, bran has a large specific surface area and a porous structure, which can stably load the active ingredients in the extract through impregnation and adsorption; under the protection of the carrier, the active ingredients have fewer opportunities to come into contact with other components in the system during production and storage, thus maintaining their biological activity for a longer period; on the other hand, after loading the extract into the bran particles, the release of active ingredients is promoted during kneading, and after the aged keratin layer is removed, the transdermal absorption of active ingredients is promoted. Therefore, the above combination of bran and extract has a synergistic effect, not a simple functional additive.

[0016] In addition, the bran particles in this invention undergo enzymatic hydrolysis-cooking pretreatment, which serves two purposes: firstly, it can appropriately soften the texture of the bran particles, reduce surface roughness, and thus improve the user experience; secondly, it can make the interior of the bran particles more porous, increase their specific surface area or porosity, and thus stably load more active ingredients from the extract.

[0017] Preferably, the preparation method of the soothing and anti-allergic bran granules includes the following steps: S1: Grind the wheat bran and sieve it to obtain wheat bran granules.

[0018] S2: Add the bran particles to an enzymatic hydrolysate containing cellulase, protease and amylase for enzymatic hydrolysis; filter and wash to obtain preliminarily softened and porous bran particles.

[0019] Wheat bran's main component is dietary fiber, which forms its framework. It also contains some protein and starch. Therefore, cellulase, protease, and amylase were selected for enzymatic hydrolysis. Specifically, since dietary fiber is the main reason for wheat bran's roughness and firm texture (providing a physical massage effect), moderate enzymatic hydrolysis of dietary fiber can minimize the roughness of the bran while maintaining its basic texture. Protein and starch are interspersed with dietary fiber in wheat bran; enzymatic hydrolysis by protease and amylase releases a large amount of space, thus giving the bran a higher load-bearing capacity.

[0020] S3: Add the bran particles obtained in S2 to water for steaming and cooking, then separate to obtain softened bran particles with pores.

[0021] Continuing to steam the bran can further reduce its surface roughness. It should be noted that, in our experiments, we found that if the bran particles are steamed before enzymatic hydrolysis, the efficiency of the enzymatic hydrolysis of dietary fiber will significantly decrease. This may be because the proteins and starches denature and gelatinize at high temperatures, respectively, and to some extent encapsulate the cellulose, making it difficult for the cellulose to fully contact the cellulase in the initial stages.

[0022] S4: The bran particles obtained in S3 are soaked in an impregnation solution containing chamomile extract, poria cocos extract and purslane extract, allowed to stand for adsorption, filtered, vacuum dried, pulverized and sieved to obtain soothing and anti-allergic bran particles.

[0023] Preferably, in S1, the particle size of the bran particles is less than 80 micrometers; in S4, the particle size of the soothing and anti-allergic bran particles is less than 100 micrometers.

[0024] After enzymatic hydrolysis and cooking, bran particles expand in volume due to the loosening process. To avoid causing pain to consumers' skin during friction, the particle size needs to be controlled. Ultimately, this invention found that a particle size of less than 100 micrometers provides a better user experience.

[0025] Preferably, in S2, the cellulase activity in the enzymatic hydrolysate is 0.2-0.4 U per gram of bran particles, the protease activity is 0.1-0.2 U per gram of bran particles, and the amylase activity is 0.1-0.2 U per gram of bran particles; the enzymatic hydrolysis temperature is 30-40℃, the pH of the enzymatic hydrolysate is 6-8, and the time is 1-3 hours.

[0026] As mentioned above, the purpose of enzymatic hydrolysis of bran particles in this invention is to moderately soften the texture and reduce surface roughness, while removing proteins and starches from the bran interior to increase its specific surface area or porosity. Therefore, the required degree of enzymatic hydrolysis for cellulase, protein, and starch differs during the process. For dietary fiber, moderate hydrolysis is necessary; insufficient hydrolysis will not effectively soften the texture or reduce roughness, while excessive hydrolysis will cause the bran skeleton to collapse. For protein and starch, it is necessary to remove them as completely as possible to release more loading space. To achieve these objectives, the degree of hydrolysis needs to be strictly controlled. Ultimately, this invention found that the above-mentioned hydrolysis conditions yielded the best results.

[0027] Preferably, in step S3, the cooking temperature is 100-130℃ and the time is 1-2 hours.

[0028] Preferably, in step S3, the solid-liquid ratio of the bran particles to water is 10-20 g / 100 mL.

[0029] Preferably, in step S4, the solid-liquid ratio of the bran particles to the impregnation liquid is 10-25 g / 100 mL; and the total content of the extract in the impregnation liquid is 1-10 g / 100 mL.

[0030] Preferably, in step S4, the solvent of the impregnation solution is water or an aqueous alcohol solution.

[0031] Preferably, in step S4, the static adsorption time is 24-36 hours; and the vacuum drying temperature is 40-60°C.

[0032] Preferably, the surfactant is selected from one or more of amino acid surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants.

[0033] Preferably, the moisturizer is selected from one or more of glycerin, sorbitol, propylene glycol and sodium hyaluronate.

[0034] Preferably, the thickener is selected from one or more of carbomer, xanthan gum, sodium chloride, and PEG-150 distearate sorbitol.

[0035] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The soothing and anti-allergic plant extract composition of the present invention uses chamomile extract, poria cocos extract and purslane extract as active ingredients. The present invention found that the above three extracts have excellent compatibility and can produce a synergistic effect of "1+1+1>3" in terms of skin soothing and anti-allergy when combined in an appropriate ratio.

[0036] (2) The present invention loads the soothing and anti-allergic plant extract composition into bran and then applies it to the facial cleanser. This can improve the activity stability of the soothing and anti-allergic plant extract in the system. At the same time, combined with the physical massage and exfoliation effect of bran, it can promote the transdermal absorption of active ingredients, thereby enhancing the soothing and anti-allergic effects of the product. Attached Figure Description

[0037] Figure 1 ROS fluorescence images of the blank group, model group, and experimental group 7; Figure 2 The effects of experimental groups 1-10 on ROS levels in zebrafish; Figure 3 The effect of experimental groups 1-10 on the relative expression level of COX-2 gene in zebrafish; Figure 4 The effect of experimental groups 1-10 on the relative expression level of TNF-α gene in zebrafish; Figure 5 The effect of experimental groups 1-10 on the relative expression level of IL-6 gene in zebrafish; Figure 6Images showing reddened facial skin on the subject (left: before use, right: after use). Detailed Implementation

[0038] First, a soothing and anti-allergic plant extract composition comprising chamomile extract, poria cocos extract and purslane extract; wherein the mass ratio of chamomile extract, poria cocos extract and purslane extract in the composition is 100:(10-30):(30-50).

[0039] Preferably, the Poria cocos extract and Portulaca oleracea extract are water extracts or alcohol-water extracts.

[0040] Secondly, a soothing and anti-allergic facial cleanser comprises the following raw materials in weight percentages: 40-60% surfactant, 3-10% moisturizer, 0.1-1.5% soothing and anti-allergic bran particles, 0.1-0.3% thickener, 0-1% preservative, 0-0.5% pH adjuster, and solvent. The soothing and anti-allergic bran particles comprise a bran particle carrier that has undergone enzymatic hydrolysis and cooking, and chamomile extract, poria cocos extract, and purslane extract loaded onto the bran particle carrier.

[0041] Preferably, the surfactant is selected from one or more of amino acid surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants. The humectant is selected from one or more of glycerin, sorbitol, propylene glycol, and sodium hyaluronate. The thickener is selected from one or more of carbomer, xanthan gum, sodium chloride, and PEG-150 distearate sorbitol. The preservative is selected from one or more of phenoxyethanol, sodium benzoate, potassium sorbate, and parabens. The pH adjuster is selected from citric acid and sodium hydroxide. The solvent is water.

[0042] Preferably, the preparation method of the soothing and anti-allergic bran granules includes the following steps: S1: Grind the wheat bran and sieve it to obtain wheat bran granules.

[0043] Preferably, in S1, the particle size of the bran particles is less than 80 micrometers.

[0044] S2: Add the bran particles to an enzymatic hydrolysate containing cellulase, protease and amylase for enzymatic hydrolysis; filter and wash to obtain preliminarily softened and porous bran particles.

[0045] Preferably, in S2, the cellulase activity in the enzymatic hydrolysate is 0.2-0.4 U per gram of bran particles, the protease activity is 0.1-0.2 U per gram of bran particles, and the amylase activity is 0.1-0.2 U per gram of bran particles; the enzymatic hydrolysis temperature is 30-40℃, the pH of the enzymatic hydrolysate is 6-8, and the time is 1-3 hours.

[0046] S3: Add the bran particles obtained in S2 to water for steaming and cooking, then separate to obtain softened bran particles with pores.

[0047] Preferably, in step S3, the cooking temperature is 100-130℃, and the time is 1-2 hours. The solid-liquid ratio of the bran particles to water is 10-20 g / 100 mL.

[0048] S4: The bran particles obtained in S3 are soaked in an impregnation solution containing chamomile extract, poria cocos extract and purslane extract, allowed to stand for adsorption, filtered, vacuum dried, pulverized and sieved to obtain soothing and anti-allergic bran particles with a particle size of less than 100 micrometers.

[0049] Preferably, in step S4, the solid-liquid ratio of the bran particles to the impregnation solution is 10-25 g / 100 mL; the total content of the extract in the impregnation solution is 1-10 g / 100 mL. The solvent of the impregnation solution is water or an alcohol-water solution.

[0050] Preferably, in step S4, the static adsorption time is 24-36 hours; and the vacuum drying temperature is 40-60°C.

[0051] (a) The effect of different extract combinations on soothing and anti-allergic effects Table 1 Weigh each ingredient according to the proportions in Table 1, mix them, and obtain the corresponding soothing and anti-sickness composition.

[0052] Among them, chamomile water extract was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-045; Poria cocos water extract was purchased from Shaanxi Bolin Biotechnology Co., Ltd.; Portulaca oleracea water extract was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-241; green tea water extract (as a similar efficacy substitute for chamomile water extract) was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-120; oat water extract (as a similar efficacy substitute for Poria cocos water extract) was purchased from Shaanxi Pinhong Biotechnology Co., Ltd.; and Centella asiatica water extract (as a similar efficacy substitute for Portulaca oleracea water extract) was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-116.

[0053] Efficacy testing (1) Anti-allergic activity test (determination of tryptase inhibition rate in zebrafish) Principle: Zebrafish mast cells, with structures and functions similar to those of mammals, participate in the body's immune and allergic responses. N-benzoyl-DL-arginine p-nitrobenzamide hydrochloride (BAPNA) is a tryptase-specific substrate, allowing for the detection of tryptase expression levels in zebrafish. Measuring the tryptase expression level and inhibition rate induced by a substance in zebrafish over 24 hours allows for quantitative analysis of the substance's sensitizing and anti-allergic efficacy.

[0054] S1: Each group of soothing and anti-allergic composition solutions (1.0 mg / mL) was prepared using distilled water as the sample group; a Compoud 48 / 80 solution (10 μg / mL) was prepared using distilled water as the model control group; and distilled water was used as the solvent control group.

[0055] S2: Select zebrafish with normal development and 3 dpf (wild AB type zebrafish, Nanjing Yishu Lihua Biotechnology Co., Ltd.) and place them in 96-well plates, 5 zebrafish per well. Add 100 μL of distilled water to the model control group and 100 μL of each group's soothing and anti-allergic composition solution to the sample group. Set up 4 replicates for each group and incubate at 28℃ for 1 h.

[0056] S3: Add 100 μL of compoud 48 / 80 solution to each well and incubate at 28 °C for 1 h.

[0057] S4: Take 100 μL of supernatant from each well and place it in another 96-well plate. Add 100 μL of BAPNA (1 mg / mL) and incubate at 38°C for 1 h.

[0058] S5: Use an ELISA reader to detect the absorbance (OD405 value) and calculate the Tryptase expression level using the following formula.

[0059] Tryptase expression level / % = (OD 样品组 - OD 溶剂组 ) / OD 溶剂组 ×100% Tryptase inhibition rate / % = (Tryptase 模型组 - Tryptase 样品组 Tryptase 模型组 ×100% The data from the above tests are shown in Table 2: Table 2 The data comparison in Table 2 shows that the soothing and anti-allergic compositions in each experimental group can reduce the amount of tryptase secretion caused by compoud 48 / 80 stimulation to a certain extent. Among them: Experimental groups 1-3 used a single extract, while experimental groups 4-6 used two extracts each. Compared with experimental group 7, which used a combination of three extracts (chamomile + poria cocos water + purslane), the results showed that the combination of "chamomile + poria cocos water + purslane" was more advantageous, indicating that these three specific extracts can produce a synergistic effect.

[0060] In experimental groups 8-10, one of the extracts in experimental group 7 was replaced with an extract that is currently recognized as having similar efficacy. The results showed that the inhibition rate of Tryptase in experimental groups 8-10 was significantly lower than that in experimental group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0061] (2) Antioxidant activity test (ROS production in zebrafish) Zebrafish embryos with normal development 24 h after fertilization were selected and randomly divided into three groups: a blank control group (supplemented with culture water only), a model group (2.0 mmol / L H2O2), and 10 experimental groups (2.0 mmol / L H2O2 + 1.0 mg / mL, experimental groups 1-10). Each well contained 20 embryos, and each group had 3 parallel wells. All groups were cultured at 28.5℃ in a light-dark incubator with a 14 h / 10 h light-dark cycle for 96 h, with the solution changed every 24 h.

[0062] After culture, the embryos were first washed with zebrafish culture water, and then stained with DCFH-DA (20 μg / mL) for 1.2 h (in the dark). After staining, the embryos were washed again with zebrafish culture water, and then anesthetized with 0.02% 3-ethoxyaniline methanesulfonate. The fluorescence intensity in the zebrafish was then observed under a stereofluorescence microscope, and the fluorescence intensity of the samples was measured using ImageJ software to analyze the amount of ROS produced in the samples.

[0063] pass Figure 1 and Figure 2 Data comparison shows that the soothing and anti-allergic compositions in each experimental group can reduce ROS produced by H2O2 stimulation in zebrafish to a certain extent, and decrease fluorescence intensity. Specifically: Experimental groups 1-3 used a single extract, while experimental groups 4-6 used two extracts each. Compared with experimental group 7, which used a combination of three extracts (chamomile + poria cocos water + purslane), the results showed that the combination of "chamomile + poria cocos water + purslane" was more advantageous, indicating that these three specific extracts can produce a synergistic effect.

[0064] In experimental groups 8-10, one of the extracts in experimental group 7 was replaced with an extract that is currently recognized as having similar efficacy. The results showed that the ability of experimental groups 8-10 to clear ROS generated in zebrafish was significantly lower than that of experimental group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0065] (3) Anti-inflammatory activity test (testing of COX-2 activity and inflammatory factors in zebrafish) Zebrafish embryos with normal development were selected 7-8 hours after fertilization. The experiment was divided into a blank group (only culture water was added), a model group (15 μg / mL LPS), and a drug treatment group (15 μg / mL LPS + 1.0 mg / mL experimental group 1-10), with 60 embryos in each group. After incubation for 72 hours, the zebrafish embryos were collected, total RNA was extracted, cDNA was reverse transcribed, genes were amplified, and the relative expression levels of COX-2, TNF-α, and IL-6 genes were measured.

[0066] COX-2 is an inducible enzyme that is highly expressed under inflammatory stimulation and plays a leading role in inflammatory responses such as pain, fever, and swelling. Interleukin-6 (IL-6) is a multifunctional cytokine produced by various cells (including lymphocytes, monocytes, and macrophages) and is widely involved in the regulation of immune and inflammatory responses. Elevated levels of tumor necrosis factor-α (TNF-α) gene expression in the blood also indicate the presence of an inflammatory response.

[0067] pass Figure 3 , Figure 4 and Figure 5 Data comparison shows that the soothing and anti-allergic compositions in each experimental group can reduce the release of inflammatory factors such as COX-2, TNF-α, and IL-6 produced in zebrafish by LPS stimulation to a certain extent. Among them: Experimental groups 1-3 used a single extract, while experimental groups 4-6 used two extracts each. Compared with experimental group 7, which used a combination of three extracts (chamomile + poria cocos water + purslane), the results showed that the combination of "chamomile + poria cocos water + purslane" was more advantageous, indicating that these three specific extracts can produce a synergistic effect.

[0068] In experimental groups 8-10, one of the extracts in experimental group 7 was replaced with an extract that is currently recognized as having similar effects. The results showed that the downregulation ability of experimental groups 8-10 on inflammatory factors in zebrafish was significantly lower than that of experimental group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0069] (3) Skin irritation test Select 8-week-old male SD rats (provided by Shanghai Slack Experimental Animal Co., Ltd., production license number: SCXK (Shanghai) 2017-0005), and shave the hair on both sides of the spine on their backs (ensure not to damage the epidermis, with an area of 3 cm × 3 cm). After 24 hours, apply 0.5 mL of the soothing and anti-allergic composition solution in the test group 7 on one side of the epidermis of the depilated area. First, cover it with gauze and cellophane, and then fix it with non-irritating adhesive tape and bandage. The other side of the epidermis serves as a control. After 4 hours, remove the covering and wash the epidermis with warm water. Observe the epidermis condition of the applied area and score it at 1 h, 24 h, 48 h, and 72 h after washing. Make a comprehensive evaluation based on the average value of the scores of the test animals, and determine the skin irritation intensity according to the highest average score at the three time points of 24 h, 48 h, and 72 h.

[0070] Among them, the criteria for classifying skin irritation intensity are as follows: average score < 0.5, non-irritating; average score 0.5 to < 2.0, slightly irritating; average score 2.0 to < 6.0, moderately irritating; average score 6.0 to 8.0, strongly irritating.

[0071] The results of the skin irritation test are shown in Table 3: Table 3 From the results in Table 3, it can be seen that the soothing and anti-allergic composition solution in test group 7 has no irritation to the back skin of rats.

[0072] (2) Application of the soothing and anti-allergic composition in facial cleanser Example 1 A soothing and anti-allergic facial cleanser, which comprises the following raw materials in mass percentages: sodium cocoyl glutamate 25%, sodium lauroamphoacetate 15%, cocamidopropyl hydroxysultaine 10%, sorbitol 4%, sodium hyaluronate 0.5%, soothing and anti-allergic bran particles 1%, carbomer 0.2%, sodium benzoate 0.5%, appropriate amount of citric acid, and the balance being deionized water.

[0073] Preparation process: (1) Preparation of soothing and anti-allergic bran particles: Grind the bran, sieve it, and obtain oat bran particles with a particle size less than 80 microns.

[0074] Add the oat bran particles to an enzymatic hydrolysis solution at a temperature of 37°C and a pH of 7 (the enzyme activity of cellulase is 0.3 U per gram of bran particles, the enzyme activity of papain is 0.15 U per gram of bran particles, and the enzyme activity of amylase is 0.15 U per gram of bran particles), and carry out enzymatic hydrolysis for 2 h under stirring conditions; after the enzymatic hydrolysis is completed, filter and wash the product to obtain preliminarily softened bran particles with pores.

[0075] The bran particles obtained in the previous step were added to a steamer containing water at a solid-liquid ratio of 15g / 100mL for steaming at 115℃ for 1.5h. After steaming, the bran particles were separated to obtain bran particles that were softened twice and had pores.

[0076] The bran granules obtained in the previous step were soaked in an aqueous solvent soaking solution (chamomile water extract (source same as test group 7) content of 3g / 100mL, poria water extract (source same as test group 7) content of 0.6g / 100mL, purslane water extract (source same as test group 7) content of 1.2g / 100mL) at a solid-liquid ratio of 20g / 100mL. After standing for adsorption for 36h, the mixture was filtered, vacuum dried at 60℃, pulverized, and sieved to obtain soothing and anti-allergic bran granules with a particle size of less than 100 micrometers.

[0077] (2) Preparation of facial cleanser: Deionized water, sorbitol, and sodium hyaluronate were added to an aqueous phase pot and stirred until completely dissolved. The mixture was then heated to 65°C to obtain an aqueous phase solution. While maintaining the temperature and stirring continuously, sodium cocoyl glutamate, sodium lauroamphoacetate, and cocamidopropyl hydroxysulfonate were slowly added to the aqueous phase solution. After the addition was complete and the mixture was stirred until no particles remained, carbomer was added to the system and stirred until homogeneous. The system was then cooled to 40°C, and soothing and anti-allergic bran granules and sodium benzoate were added and stirred slowly until homogeneous. The pH of the system was adjusted to 6.5 with citric acid. The mixture was then slowly cooled to 35°C while stirring until it became a fine paste. After pouring the paste into a container and allowing it to cool naturally, the facial cleanser was obtained.

[0078] Comparative Example 1 (Blank Cleansing Cream) The difference between this comparative example and Example 1 is that no soothing and anti-allergic bran particles are added, and the missing amount is made up with deionized water.

[0079] Comparative Example 2 (The extract and bran particles were added separately) The difference between this comparative example and Example 1 is that the extracts and bran particles are added separately, as follows: The formula includes the following ingredients by weight percentage: sodium cocoyl glutamate 25%, sodium lauroyl amphoteric acid 15%, cocamidopropyl hydroxysulfonate 10%, sorbitol 4%, sodium hyaluronate 0.5%, wheat bran granules 0.9%, chamomile water extract 0.06%, poria cocos water extract 0.012%, purslane water extract 0.024%, carbomer 0.2%, sodium benzoate 0.5%, citric acid as needed, and deionized water as the balance.

[0080] Preparation process: (1) Preparation of bran pellets: The bran was ground and sieved to obtain oat bran particles with a particle size of less than 80 micrometers.

[0081] Oat bran particles were added to an enzymatic hydrolysate at 37°C and pH 7 (with cellulase activity of 0.3 U per gram of bran particles, papain activity of 0.15 U per gram of bran particles, and amylase activity of 0.15 U per gram of bran particles) and enzymatically hydrolyzed for 2 hours under stirring. After hydrolysis, the mixture was filtered and the product was washed to obtain preliminarily softened and porous bran particles.

[0082] The bran particles obtained in the previous step were added to a steamer containing water at a solid-liquid ratio of 15g / 100mL for steaming at 115℃ for 1.5h. After steaming, the bran particles were separated, vacuum dried at 60℃, pulverized, and sieved to obtain secondary softened bran particles with a particle size of less than 100 micrometers and with pores.

[0083] (2) Preparation of facial cleanser: Deionized water, sorbitol, and sodium hyaluronate were added to an aqueous phase pot and stirred until completely dissolved. The mixture was then heated to 65°C to obtain an aqueous solution. While maintaining the temperature and stirring continuously, sodium cocoyl glutamate, sodium lauroamphoacetate, and cocamidopropyl hydroxysulfonate were slowly added to the aqueous solution. After the addition was complete and the mixture was stirred until no particles remained, carbomer was added to the system and stirred until homogeneous. The system was then cooled to 40°C, and bran granules, chamomile water extract, poria cocos water extract, purslane water extract, and sodium benzoate were added and stirred slowly until homogeneous. The pH of the system was adjusted to 6.5 with citric acid. The mixture was then slowly cooled to 35°C while stirring until it became a fine paste. After pouring the paste into a container and allowing it to cool naturally, the facial cleanser was obtained.

[0084] Comparative Example 3 (wheat bran particles without enzymatic hydrolysis) The difference between this comparative example and Example 1 is that the bran particles are not subjected to enzymatic hydrolysis. The specific procedure is as follows: Formula: Same as Example 1.

[0085] Preparation process: (1) Preparation of soothing and anti-allergic bran granules: The bran was ground and sieved to obtain oat bran particles with a particle size of less than 80 micrometers.

[0086] Oat bran particles were added to a steamer containing water at a solid-liquid ratio of 15g / 100mL and steamed at 115℃ for 1.5 hours. The bran particles were then separated to obtain bran particles.

[0087] The bran granules obtained in the previous step were soaked in an aqueous solvent soaking solution (chamomile water extract (source same as test group 7) content of 3g / 100mL, poria water extract (source same as test group 7) content of 0.6g / 100mL, purslane water extract (source same as test group 7) content of 1.2g / 100mL) at a solid-liquid ratio of 20g / 100mL. After standing for adsorption for 36h, the mixture was filtered, vacuum dried at 60℃, pulverized, and sieved to obtain soothing and anti-allergic bran granules with a particle size of less than 100 micrometers.

[0088] (2) Preparation of facial cleanser: Same as in Example 1.

[0089] Comparative Example 4 (wheat bran pellets were not cooked) The difference between this comparative example and Example 1 is that the bran particles are not subjected to steaming or cooking treatment. The specific procedure is as follows: Formula: Same as Example 1.

[0090] Preparation process: (1) Preparation of soothing and anti-allergic bran granules: The bran was ground and sieved to obtain oat bran particles with a particle size of less than 80 micrometers.

[0091] Oat bran particles were added to an enzymatic hydrolysate at 37°C and pH 7 (with cellulase activity of 0.3 U per gram of bran particles, papain activity of 0.15 U per gram of bran particles, and amylase activity of 0.15 U per gram of bran particles) and enzymatically hydrolyzed for 2 hours under stirring. After hydrolysis, the mixture was filtered and the product was washed to obtain preliminarily softened and porous bran particles.

[0092] The bran granules obtained in the previous step were soaked in an aqueous solvent soaking solution (chamomile water extract (source same as test group 7) content of 3g / 100mL, poria water extract (source same as test group 7) content of 0.6g / 100mL, purslane water extract (source same as test group 7) content of 1.2g / 100mL) at a solid-liquid ratio of 20g / 100mL. After standing for adsorption for 36h, the mixture was filtered, vacuum dried at 60℃, pulverized, and sieved to obtain soothing and anti-allergic bran granules with a particle size of less than 100 micrometers.

[0093] (2) Preparation of facial cleanser: Same as in Example 1.

[0094] Comparative Example 5 (wheat bran particles were first steamed and then enzymatically hydrolyzed) The difference between this comparative example and Example 1 is that the bran particles are first steamed and then enzymatically hydrolyzed. The specific procedure is as follows: Formula: Same as Example 1.

[0095] Preparation process: (1) Preparation of soothing and anti-allergic bran granules: The bran was ground and sieved to obtain oat bran particles with a particle size of less than 80 micrometers.

[0096] The oat bran particles obtained in the previous step were added to a steamer containing water at a solid-liquid ratio of 15g / 100mL for steaming at 115℃ for 1.5h. After steaming, the bran particles were separated to obtain softened bran particles.

[0097] The bran particles obtained in the previous step were added to an enzymatic hydrolysate at 37°C and pH 7 (with cellulase activity of 0.3 U per gram of bran particles, papain activity of 0.15 U per gram of bran particles, and amylase activity of 0.15 U per gram of bran particles), and enzymatic hydrolysis was carried out for 2 hours under stirring conditions. After the enzymatic hydrolysis was completed, the product was filtered and washed to obtain secondary softened and porous bran particles.

[0098] The bran granules obtained in the previous step were soaked in an aqueous solvent soaking solution (chamomile water extract (source same as test group 7) content of 3g / 100mL, poria water extract (source same as test group 7) content of 0.6g / 100mL, purslane water extract (source same as test group 7) content of 1.2g / 100mL) at a solid-liquid ratio of 20g / 100mL. After standing for adsorption for 36h, the mixture was filtered, vacuum dried at 60℃, pulverized, and sieved to obtain soothing and anti-allergic bran granules with a particle size of less than 100 micrometers.

[0099] (2) Preparation of facial cleanser: Same as in Example 1.

[0100] Comparative Example 6 (wheat bran granules without enzymatic hydrolysis-cooking treatment) The difference between this comparative example and Comparative Example 2 is that the bran particles do not undergo enzymatic hydrolysis-cooking treatment. The specific procedure is as follows: Formula: Same as comparative example 2.

[0101] Preparation process: (1) Preparation of bran pellets: The bran was ground and sieved to obtain oat bran particles with a particle size of less than 100 micrometers.

[0102] (2) Preparation of facial cleanser: Same as comparative example 2.

[0103] Human trials (1) Human skin patch test Test substances: Cleansing cream samples obtained in Example 1 and Comparative Examples 1-6.

[0104] Participants: 20 women and 20 men. Age: 20-50 years old. Health status: Participants had healthy skin and no history of skin diseases or allergies.

[0105] Patch Method: 20 mg of the test substance (facial cleanser sample) was evenly added to the chamber of the patch applicator. The patch applicator containing the test substance was then applied to the volunteer's back using non-irritating adhesive tape. Gently press the applicator onto the skin to ensure even application. The patch applicator remained in place for 24 hours. A blank control well was used (no substance was placed in the control well). The patch applicator was removed after a 30-minute interval, and skin reaction was observed after the indentation disappeared. Observations were taken at 24 and 48 hours post-patch testing.

[0106] The results were interpreted in accordance with the Cosmetic Hygiene Standards (2015 edition). The results showed that none of the tested samples exhibited adverse reactions such as skin rashes, papules, or blisters. This indicates that the samples in each case were mild and non-irritating.

[0107] (2) User experience test Test substances: Cleansing cream samples obtained in Example 1 and Comparative Examples 1-6.

[0108] Participants: 15 women and 15 men. Age: 20-50 years old. Health status: Participants had healthy skin and no history of skin diseases or allergies.

[0109] Test method: Take 1g of the test product cleansing cream sample into the palm of your hand, add about 20mL of water and knead until smooth. Then apply it to the face and massage with your palm for 1 minute. Score according to the user experience evaluation criteria in Table 4.

[0110] Table 4: User Experience Evaluation Criteria The test results are shown in Table 5.

[0111] Table 5: Results of User Experience Test As shown in Table 5, in Comparative Example 6, the bran particles were not subjected to enzymatic hydrolysis-cooking treatment, resulting in significant physical pain during massage. This is due to the hard texture and rough surface of the bran particles. In contrast, in Example 1, the bran particles underwent enzymatic hydrolysis-cooking treatment, which softened the texture and largely eliminated the roughness, thus improving the user experience. In Comparative Examples 3-4, only single enzymatic hydrolysis or cooking treatment was performed, resulting in limited softening and reduction in roughness, leading to a less than ideal user experience. In Comparative Example 5, the order of enzymatic hydrolysis and cooking was changed. Since cooking before enzymatic hydrolysis affects the efficiency of subsequent hydrolysis, the user experience was inferior to that of Example 1.

[0112] (3) Human efficacy test Test substances: Cleansing cream samples obtained in Example 1 and Comparative Examples 1-2; all samples were stored at room temperature for 3 months in advance (the purpose of which was to partially deactivate the active ingredients in Comparative Example 2 during storage).

[0113] Subject population: Sixty healthy subjects aged 20-50 years were selected. Volunteers with obvious erythema on their cheeks, nose and other areas at rest were randomly divided into 3 groups.

[0114] The testing period was 28 days. Data was collected from subjects after 28 consecutive days of use in a temperature and humidity controlled chamber. During the testing period, the product was used once daily, and other soothing hair care products should not be used simultaneously. On days 0 and 28 of use, researchers collected data on the subjects' skin stratum corneum moisture content, transepidermal water loss rate, and skin hemoglobin content.

[0115] Table 6: Changes in skin measurement indicators before and after product use As shown in Table 6, after 28 days of continuous use of the sample from Example 1, the stratum corneum moisture content of the skin was significantly increased, transepidermal water loss (TWEL) was significantly reduced, and the skin barrier function was significantly repaired. However, the effects were not significant for subjects using Comparative Example 1 and Comparative Example 2. Simultaneously, images of the facial redness area collected by Visia-CR showed that subjects who used the sample from Example 1 for 28 days exhibited a significant decrease in facial redness. Figure 6 ).

[0116] The above comparison demonstrates that loading each extract into bran particles significantly improves its activity and stability, thus resulting in more pronounced soothing and anti-inflammatory effects.

Claims

1. A soothing and anti-allergic plant extract composition, characterized in that: The composition includes chamomile extract, poria cocos extract and purslane extract; the mass ratio of chamomile extract, poria cocos extract and purslane extract in the composition is 100:(10-30):(30-50).

2. The soothing and anti-allergic plant extract composition as described in claim 1, characterized in that: The Poria cocos extract and Portulaca oleracea extract are water extracts or alcohol-water extracts.

3. A soothing and anti-allergic facial cleanser containing the soothing and anti-allergic plant extract composition according to claim 1 or 2, characterized in that: The raw materials include the following percentages by weight: surfactant 40-60%, humectant 3-10%, soothing and anti-allergic bran granules 0.1-1.5%, thickener 0.1-0.3%, preservative 0-1%, pH adjuster 0-0.5%, and solvent; The soothing and anti-allergic bran granules include bran granules that have undergone enzymatic hydrolysis and cooking, as well as chamomile extract, poria cocos extract and purslane extract loaded onto the bran granules.

4. The soothing and anti-allergic facial cleanser as described in claim 3, characterized in that: The preparation method of the soothing and anti-allergic bran granules includes the following steps: S1: Grind the wheat bran and sieve it to obtain wheat bran granules; S2: Add the bran particles to an enzymatic hydrolysate containing cellulase, protease, and amylase for enzymatic hydrolysis; filter and wash to obtain preliminarily softened and porous bran particles; The enzymatic hydrolysate contains 0.2-0.4 U of cellulase per gram of bran particle, 0.1-0.2 U of protease per gram of bran particle, and 0.1-0.2 U of amylase per gram of bran particle; the enzymatic hydrolysis temperature is 30-40℃, the pH of the enzymatic hydrolysate is 6-8, and the time is 1-3 hours. S3: Add the bran particles obtained in S2 to water for steaming and cooking, then separate to obtain softened bran particles with pores. S4: The bran particles obtained in S3 are soaked in an impregnation solution containing chamomile extract, poria cocos extract and purslane extract, allowed to stand for adsorption, filtered, vacuum dried, pulverized and sieved to obtain soothing and anti-allergic bran particles with a particle size of less than 100 micrometers.

5. The soothing and anti-allergic facial cleanser as described in claim 4, characterized in that: In S1, the particle size of the bran particles is less than 80 micrometers.

6. The soothing and anti-allergic facial cleanser as described in claim 4, characterized in that: In S3 The cooking temperature is 100-130℃, and the time is 1-2 hours; The solid-liquid ratio of the bran particles to water is 10-20 g / 100 mL.

7. The soothing and anti-allergic facial cleanser as described in claim 4, characterized in that: In S4, The solid-liquid ratio of the bran particles to the soaking liquid is 10-25 g / 100 mL; The total content of the extract in the impregnation solution is 1-10g / 100mL.

8. The soothing and anti-allergic facial cleanser as described in claim 4 or 7, characterized in that: In S4, the solvent of the impregnation solution is water or an aqueous alcohol solution.

9. The soothing and anti-allergic facial cleanser as described in claim 4 or 7, characterized in that: In S4, The static adsorption time is 24-36 hours; The vacuum drying temperature is 40-60℃.

10. The soothing and anti-allergic facial cleanser as described in claim 3, characterized in that: The surfactant is selected from one or more of amino acid surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants; The moisturizer is selected from one or more of glycerin, sorbitol, propylene glycol, and sodium hyaluronate; The thickener is selected from one or more of carbomer, xanthan gum, sodium chloride, and PEG-150 distearate sorbitol.

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