Whitening and spot-fading composition based on fermented jasmine flower extract

Abstract

The invention discloses a whitening and spot-fading composition based on a fermented jasmine flower extract. The whitening and spot-fading composition comprises the following components in parts by mass: 0.1-1 part of a nano emulsifier, 0.5-8 parts of the fermented jasmine flower extract, 0.5-1.5 parts of arbutin and 1-3 parts of tranexamic acid. The nano emulsifier is a mixture of hydrogenated lecithin and phytosterol, the mass fraction ratio of arbutin to tranexamic acid is 0.5: 1, and the fermented jasmine flower extract specifically comprises a jasmine flower extract, a lactobacillus fermentation extract, water, 1, 2-hexanediol and 1, 2-pentanediol. The average particle size of the emulsion is about 256.56 nm through a nano emulsification technology, and compared with a conventional system, the transdermal absorption efficiency of active ingredients such as the jasmine flower extract, arbutin and tranexamic acid can be greatly improved, so that the active ingredients can reach action targets more easily; the problem of non-uniform dispersion caused by component solubility difference can be solved, the active component analysis risk is remarkably reduced, the system stability is improved, and the whitening and spot fading effects are enhanced.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a simple-to-use nanoemulsification technology combined with specific plant active ingredients to achieve a highly effective whitening and spot-fading composition and its preparation method. Background Technology

[0002] With rising living standards and increased skincare awareness, consumers' demand for whitening and spot-fading cosmetics is growing, especially among those with sensitive skin. They not only require products with excellent whitening and spot-fading effects, but also demand strict requirements for gentleness, safety, and stability. The formation mechanism of skin pigmentation is complex, mainly related to factors such as excessive activation of melanocytes, abnormally elevated tyrosinase activity, oxidative stress induced by ultraviolet radiation, and inflammatory responses. Therefore, developing gentle whitening compositions that can precisely target the melanin production pathway and also have repairing effects has become a research hotspot in the cosmetics field.

[0003] Currently, most whitening and spot-fading cosmetics on the market achieve their effects by adding active ingredients such as arbutin, tranexamic acid, vitamin C and its derivatives. Among them, arbutin can inhibit tyrosinase activity to reduce melanin synthesis, and tranexamic acid can inhibit the proliferation of melanocytes and the transport of melanosomes. Both are recognized as highly effective whitening ingredients.

[0004] However, traditional whitening compositions have many technical bottlenecks: active ingredients in emulsions with conventional particle sizes (usually greater than 500nm) have difficulty penetrating the skin's stratum corneum barrier, resulting in low transdermal absorption efficiency and failure to fully exert their effects; on the other hand, some products increase the concentration of active ingredients in pursuit of rapid effects, or use highly irritating emulsifiers to improve dispersibility, which can easily cause adverse reactions such as skin redness and stinging, and are especially unsuitable for sensitive skin.

[0005] To address the issue of low absorption efficiency of active ingredients, nanoemulsion technology is increasingly being applied in the cosmetics industry. Due to their small particle size (typically less than 500 nm) and large specific surface area, nanoemulsions can effectively improve the dispersion uniformity and transdermal penetration of active ingredients, while reducing the risk of their precipitation.

[0006] However, existing nanoemulsification technologies used in whitening products still have shortcomings: First, some nanoemulsifiers have complex compositions and questionable safety, or require special co-emulsifiers to stabilize the system, increasing the complexity of product formulations; second, the preparation of nanoemulsions often requires stringent process conditions such as high shear rates and long-term homogenization, which not only consumes a lot of energy but also makes it difficult to achieve large-scale industrial production, thus limiting their widespread application in the cosmetics field.

[0007] In the selection of active ingredients, natural plant extracts are highly favored due to their gentleness. Jasmine, as a traditional medicinal plant, has certain anti-inflammatory and soothing effects. However, extracts obtained through conventional extraction methods have low levels of active ingredients and are prone to carrying plant-derived impurities. Direct application in cosmetics can easily lead to decreased stability or allergic reactions. Fermentation technology can optimize the composition of plant extracts through microbial metabolism, improving the purity and bioactivity of active ingredients while reducing irritation. However, research on the application of fermented jasmine flower extract combined with arbutin and tranexamic acid, along with nanoemulsification technology, in whitening and spot-fading repair products has not yet been reported.

[0008] In addition, the pH stability range of existing whitening and spot-fading products is relatively narrow, with most being stable under neutral or weakly alkaline conditions. However, the skin surface is weakly acidic (pH 4.5-6.0). When the product's pH value does not match the skin's physiological environment, it will not only damage the skin barrier function but may also lead to the degradation of active ingredients, further reducing efficacy.

[0009] Therefore, developing a whitening and spot-fading repair composition that employs a safe and stable nanoemulsion system, combines highly efficient and gentle compound active ingredients, and maintains good stability within the skin's physiological pH range has become an urgent problem to be solved in the field of cosmetic technology. Summary of the Invention

[0010] The purpose of this invention is to solve the problems existing in the prior art, and to propose a whitening and spot-fading composition based on fermented jasmine flower extract.

[0011] To achieve the above objectives, the present invention adopts the following technical solution: A skin-whitening and spot-fading composition based on fermented jasmine flower extract, comprising the following components in parts by weight: 0.1-1 parts nano-emulsifier, 0.5-8 parts fermented jasmine flower extract, 0.5-1.5 parts arbutin, and 1-3 parts tranexamic acid.

[0012] Furthermore, the nanoemulsifier is a mixture of hydrogenated lecithin and phytosterols, and the mass fraction ratio of arbutin to tranexamic acid is 0.5:1.

[0013] Based on the aforementioned scheme, the fermented jasmine flower extract is specifically composed of: jasmine flower extract, lactic acid bacteria fermentation extract, water, 1,2-hexanediol, and 1,2-pentanediol.

[0014] As a further embodiment of the present invention, the fermented jasmine flower extract contains ≥3% jasmine flower extract.

[0015] Furthermore, the ratio of the nanoemulsifier to the fermented jasmine flower extract is (0.1-1):1.

[0016] Furthermore, the ratio of the fermented jasmine flower extract to arbutin is (0.5-3):1.

[0017] Further, 0.05-0.2 parts of chelating agent; 0.15-1 part thickener; 0.1-5 parts humectant; 0.1-5 parts preservative; 5-20 parts polyols; 2-20 parts moisturizer; 0.5-10 parts skin conditioning agent; 0.1-5 parts antioxidant; 0.1-2 parts pH adjuster; Water is added to the remaining amount.

[0018] Furthermore, the polyol is selected from any one or at least two combinations of glycerol, butanediol, 1,3-propanediol, propylene glycol, dipropylene glycol, methylpropanediol, 1,2-pentanediol, and 1,2-hexanediol.

[0019] Furthermore, the polyol content is 5-20 parts by mass, and the mass percentage of polyol and nano-emulsifier is (5-20):1.

[0020] Furthermore, the composition is prepared using nanoemulsification technology, and the pH of the composition is 5.0-6.5.

[0021] The beneficial effects of this invention are as follows: Compared with existing technologies, the present invention has the following significant advantages: This invention is the first to construct a composite formula with "lactic acid bacteria fermented jasmine flower extract" as the core, forming a precise synergistic effect with a specific nano-emulsifier, arbutin, tranexamic acid, and polyol system. It innovatively constructs a composite system of "fermented jasmine flower extract, arbutin, and tranexamic acid": fermentation technology enhances the activity of jasmine flower extract and reduces irritation, thereby enhancing anti-inflammatory and soothing effects; the optimized ratio of the three components inhibits melanin production through multiple pathways while also providing soothing and repairing synergy, balancing high-efficiency whitening with gentleness, making it suitable for sensitive skin.

[0022] Hydrogenated lecithin and phytosterols form a biomimetic liposome structure, which, when combined with fermentation extracts at a ratio of (0.1–1):1, significantly enhances the loading rate of lipid-soluble active ingredients and the skin barrier penetration ability. This ratio far exceeds the amount of traditional emulsifiers used, achieving "high efficacy at low concentrations." Arbutin and tranexamic acid, combined at a ratio of 0.5:1, can maximize the inhibition of both tyrosinase activity and inflammatory pigmentation pathways. This ratio is significantly more effective than common ratios such as 1:1 or 2:1, exhibiting unexpected synergistic effects.

[0023] By using nanoemulsification technology, the average particle size of the emulsion is approximately 256.56 nm. Compared with conventional systems, this significantly improves the transdermal absorption efficiency of active ingredients such as jasmine flower extract, arbutin, and tranexamic acid, making it easier for them to reach their target sites. It also solves the problem of uneven dispersion caused by differences in the solubility of the ingredients, significantly reduces the risk of active ingredient leaching, improves system stability, and enhances the whitening and spot-fading effects. Utilizing natural nanoemulsifiers, with hydrogenated lecithin containing ≥75% phosphatidylcholine, the system can be stabilized without the need for co-emulsifiers, enhancing its gentleness and safety. Simultaneously, the optimized preparation process eliminates the reliance on high-energy-consuming and demanding processes inherent in traditional nanoemulsions, reducing power consumption and facilitating large-scale production, thus overcoming bottlenecks in industrial applications.

[0024] The (5–20):1 ratio of polyol to nanoemulsifier maintains emulsion stability while providing excellent moisturizing and film-forming properties. The pH 5.0–6.5 provides a physiologically weakly acidic environment for the skin, optimizing the stability of fermentation extracts and nanoemulsifiers. At the same time, it avoids the use of sulfites, completely avoiding their defects such as sensitization, discoloration, and incompatibility with vitamin C derivatives, achieving a breakthrough in both "high efficiency and safety".

[0025] This composition exhibits good stability within a pH range of 5.0-6.5, and no stratification or precipitation was observed during accelerated testing. It is suitable for various formulations such as lotions, serums, and creams, and can be widely used in daily skincare products to meet the needs of people with sensitive skin for gentle and effective whitening and spot-fading. Attached Figure Description

[0026] Figure 1 The image shown is from before using Example 1; Figure 2 Figures showing usage of Example 1 for 28 days; Figure 3 A partial view of the VISIA test before using Example 1; Figure 4 Partial view of VISIA test results after using Example 1 for 28 days; Figure 5 This is the average particle size distribution diagram for Example 1; Figure 6 This is the average particle size distribution diagram for Example 2; Figure 7 Electron microscope images and particle size distribution diagrams of Example 1; Figure 8 The electron microscope images and particle size distribution diagrams for Example 2 are shown. Detailed Implementation

[0027] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0028] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this patent, and should not be construed as limiting this patent. Those skilled in the art can understand the specific meaning of the above terms in this patent according to the specific circumstances.

[0029] Reference Figure 1-4 A whitening and spot-fading composition based on fermented jasmine flower extract, the core active components of which include: 0.1-1 parts of nano-emulsifier, 0.5-8 parts of fermented jasmine flower extract, 0.5-1.5 parts of arbutin, and 1-3 parts of tranexamic acid. The specific composition of fermented jasmine flower extract is: jasmine flower extract, lactic acid bacteria fermentation extract, water, 1,2-hexanediol, and 1,2-pentanediol. The content of jasmine flower extract in fermented jasmine flower extract is ≥3%. The ratio of nanoemulsifier to fermented jasmine flower extract is (0.1-1):1, the ratio of fermented jasmine flower extract to arbutin is (0.5-3):1, and the mass fraction ratio of arbutin to tranexamic acid is 0.5:1. It also includes the following components in parts by weight: 0.05-0.2 parts chelating agent 0.15-1 part thickener 0.1-5 parts moisturizer 0.1-5 parts preservative 5-20 parts polyols 2-20 parts moisturizer 0.5-10 parts skin conditioning agent 0.1-5 parts antioxidant 0.1-2 parts pH adjuster Water replenishment to the remaining amount; The polyol is selected from any one or at least two combinations of glycerol, butanediol, 1,3-propanediol, propylene glycol, dipropylene glycol, methylpropanediol, 1,2-pentanediol, and 1,2-hexanediol. The polyol content is 5-20 parts by mass, and the mass percentage of polyol to nano-emulsifier is (5-20):1. After production, the pH of the composition is 5.0-6.5.

[0030] The processing technology is as follows: It employs nano-emulsification technology, which has two key aspects: I. Optimize the proportions; First, dissolve the nano-emulsifier with polyol and emollient, heat to 70-85℃, and start homogenization at a speed of 5000-10000 rpm for 3-7 minutes, which is the A phase. Water, thickener, humectant, chelating agent, and preservative are mixed evenly and heated to 70-85℃ to form phase B; Then add phase B to phase A, homogenize at 5000-10000 rpm for 5-15 minutes until well mixed; then cool down, add the remaining raw materials, and mix well.

[0031] Specifically, in the compositions of the present invention with nanoparticle sizes: In nano-emulsifiers, hydrogenated lecithin contains ≥70% phosphatidylcholine, giving it extremely excellent emulsifying properties. It can emulsify any type of oil when used alone, without considering HLB value. Moreover, it can be used to prepare nano-emulsions. When used as an emulsifier, the preparation process is simple and convenient, with excellent stability, a skin-friendly and soft feel, and it can help the active ingredients penetrate. Fermented jasmine flower extract consists of jasmine flower extract, lactic acid bacteria fermentation products, 1,2-pentanediol, and 1,2-hexanediol.

[0032] Using a natural fermentation process, it retains its plant fragrance while enriching more small molecule peptide products. It contains 198 kinds of peptides with an average length of 4-6 amino acids. They are mainly small molecule peptides, which can be more easily absorbed by human skin. In addition, it also contains some flavonoids and polyphenols.

[0033] MITF is a major regulator of melanin production and the ultimate target of multiple signal transduction pathways. Fermented jasmine flower extract mainly regulates MITF expression through the MC1R and Wnt signaling pathways, thereby inhibiting melanin production.

[0034] Fermented jasmine flower extract acts as the first messenger, binding to the receptor MC1R on the cell membrane in coordination with the ASIP signaling protein. Subsequently, under the action of adenylate cyclase, it promotes the production of the second messenger cAMP in melanocytes. Under the action of cAMP, downstream effectors such as PKA and CREB inhibit the activity of tyrosinase, reduce melanin synthesis, and thus inhibit the melanin production process, thereby achieving a whitening effect.

[0035] Meanwhile, fermented jasmine flower extract can also reduce the expression of MITF through the Wnt signaling pathway, thereby inhibiting the activity of tyrosinase and reducing melanin synthesis.

[0036] In addition, fermented jasmine flower extract can significantly inhibit the expression of melanin-related enzyme genes such as TYR, TYRP1, TRP1, and TRP2, reduce the entry of melanin-related enzymes into melanosomes, inhibit the production of tannins and melanin, and thus reduce the mixed pigments generated by the interaction of tannins and melanin, achieving whitening and anti-inflammatory effects through multiple pathways.

[0037] Arbutin and tranexamic acid, as two classic whitening ingredients, can synergistically enhance the effects of fermented jasmine extract. Arbutin reduces melanin production by competitively inhibiting tyrosinase activity. Tranexamic acid blocks the transport of melanin to keratinocytes, thus preventing pigment deposition.

[0038] When combined with fermented jasmine flower extract, the three components work synergistically at different stages: fermented jasmine flower extract regulates signaling pathways to inhibit MITF expression and reduce tyrosinase production; arbutin directly inhibits tyrosinase activity; and tranexamic acid prevents melanin deposition. This multi-target synergistic effect can achieve a more comprehensive whitening and repairing effect.

[0039] II. Synergistic optimization design of active ingredients; Components Function Coordination mechanism Fermented jasmine extract Inhibit melanin production and enhance whitening By regulating MITF expression through the MC1R and Wnt signaling pathways, it reduces tyrosinase activity and, together with arbutin and tranexamic acid, covers the key upstream (MITF expression), midstream (tyrosinase activity), and downstream (melanin transport) links of melanin production. Arbutin Competitive inhibition of tyrosinase activity It works synergistically with fermented jasmine flower extract by regulating MITF expression, jointly inhibiting melanin production from both the source and activity levels of tyrosinase. Tranexamic acid Prevent melanin from being transported into keratinocytes Together with arbutin, it covers two key links in tyrosinase activity and melanin transport, while also working with the anti-inflammatory effects of fermented jasmine extract to reduce inflammation-induced pigmentation. Table of synergistic principles of active ingredients The experimental data are as follows: Deionized water Add to 100 Disodium EDTA 0.05 copies Acrylic (ester) crosspolymers / C10-30 alkanol acrylate crosspolymers 0.15 copies Allantoin 0.2 copies Inositol 0.5 copies betaine 0.5 copies p-Hydroxyacetophenone 0.4 copies Nanoemulsifiers (hydrogenated lecithin and phytosterols) 0.5 copies 1,2-Pentanediol 2.00 copies 1,3-Propanediol 3.00 copies Butylene glycol 4.00 copies Stearyl glycyrrhizin ester 0.1 copies Coconut oil alcohol-caprylic / capric acid ester 2.00 copies C13-C16 isoparaffins 2.00 copies Sodium polyacrylamide dimethyl taurate 0.40 copies Japanese cedar bud extract 0.50 copies Fermented jasmine flower extract 3.00 copies Carnosine 0.20 copies Arbutin 0.80 copies Niacinamide 3.00 copies Citric acid 0.05 copies Example 1, Example 2, Comparative Examples 1-3.

[0040] Example 1 and Example 2 each provide an emulsifier composition, and Comparative Example 1, Comparative Example 2 and Comparative Example 3 each provide active ingredient compositions with different proportions. The formulation table is shown below.

[0041]

[0042] The composition is prepared as follows: Add each ingredient of phase A to the main pot according to the weight percentage of the raw materials, stir and heat to 75-85℃, and set aside.

[0043] Add each raw material of phase B to the water pot according to the weight percentage of the raw materials, stir and heat to 75-85℃, and set aside.

[0044] Add phase B to phase A and begin homogenization at a speed of 6000 rpm for 5 minutes.

[0045] After homogenization, begin cooling and add the C-phase raw materials in sequence, mixing thoroughly.

[0046] The preparation methods of the compositions of Comparative Examples 1-3, Example 2, and Blank Example are the same as those of Example 1.

[0047] The difference between Example 1 and Example 2 is that Example 1 contains the nano-emulsifier of the present invention, wherein the phosphatidylcholine content in the hydrogenated lecithin is ≥70%; Example 2 does not contain the nano-emulsifier of the present invention, but uses a hydrogenated lecithin as an emulsifier with a phosphatidylcholine content of about 25%.

[0048] The difference between Example 1 and Comparative Example 1 is that Comparative Example 1 does not contain arbutin or tranexamic acid.

[0049] The difference between Example 1 and Comparative Example 2 is that Comparative Example 2 does not contain arbutin.

[0050] The difference between Example 1 and Comparative Example 3 is that the Comparative Example does not contain tranexamic acid.

[0051] The difference between Example 1 and the blank example is that the blank example does not contain fermented jasmine flower extract, arbutin, or tranexamic acid.

[0052] Examples 1 and 2 provide a comparison of a combination of nanoemulsifier, fermented jasmine flower extract, arbutin, and tranexamic acid, and a combination of hydrogenated lecithin, fermented jasmine flower extract, arbutin, and tranexamic acid. The microstructure and whitening effect of adding the same amount of whitening and spot-fading active ingredients under the same emulsification particle size of ordinary emulsifier and nanoemulsifier were tested.

[0053] This embodiment 1 and comparative examples 1, 2, and 3 provide a synergistic effect of arbutin and tranexamic acid on the whitening and spot-fading effects of fermented jasmine flower extract.

[0054] Structural testing To visually demonstrate the structural differences between nanoemulsifiers (hydrogenated lecithin and phytosterols) and ordinary emulsions, Examples 1 and 2 were analyzed using electron microscopy and a particle size analyzer. The results are shown in the table below. Figures 5-8 .

[0055] Particle size and PDI data tables for Examples 1 and 2 Note: The PDI (polydispersity index) value is usually obtained by dynamic light scattering (DLS) and reflects the width and uniformity of the particle size distribution.

[0056] The PDI value typically ranges from 0 to 1. PDI=0: indicates complete monodispersity, meaning all particles are exactly the same size; PDI < 0.1: Highly monodisperse (extremely uniform size). This is an ideal emulsion state.

[0057] 0.1 < PDI < 0.2: Narrow distribution. It is generally considered that the preparation process is good and the stability is relatively high.

[0058] 0.2 < PDI < 0.5: Medium distribution.

[0059] PDI > 0.5: Wide distribution (polydisperse), and there may be droplets with extremely uneven sizes or aggregation occurs.

[0060] The structural differences between Example 1 (nanoemulsifier: hydrogenated lecithin and phytosterol) and Example 2 (ordinary hydrogenated lecithin) can be intuitively reflected by the particle size and PDI data: The average particle size of the emulsion in Example 1 is only 256.56 nm, belonging to a typical nanoemulsion, and the PDI value is 15.75%, indicating excellent particle size uniformity in the system and a stable dispersion state; The particle size of Example 2 is as high as 3423.56 nm (about 3.4 μm), belonging to a conventional micron-sized emulsion, and the PDI value is 24.56%, indicating that the particle size difference is relatively significant, the distribution width is relatively large, and the system uniformity is relatively poor.

[0061] The core reason for this structural difference lies in the performance and ratio design of the emulsifier: In the nanoemulsifier selected in Example 1, the phosphatidylcholine content of hydrogenated lecithin is ≥70%. Without a co-emulsifier, fine and uniform nanoparticles can be formed through an optimized process (polyol, emollient dissolution, specific temperature and homogenization conditions). Its structure has good compatibility with the skin barrier and can reduce particle aggregation; However, the phosphatidylcholine content of the ordinary hydrogenated lecithin in Example 2 is only about 25%, and its emulsifying ability is limited. It cannot achieve nano-scale dispersion, resulting in particle aggregation and growth, forming a conventional emulsion with relatively uneven sizes. This structural difference directly affects the subsequent efficacy.

[0062] The nano-scale particles in Example 1 have a large specific surface area, which can improve the dispersion uniformity and transdermal absorption efficiency of active ingredients. The particle size of Example 2 is relatively large, and it is difficult for the particles to penetrate the stratum corneum of the skin. Moreover, uneven dispersion easily leads to the precipitation of active ingredients, which not only affects the efficacy but also reduces the stability of the system.

[0063] Functional testing The compositions prepared in the above examples and comparative examples were tested as follows.

[0064] Test 1: Patch test Test method: Take 0.02 g of each sample and apply it evenly on a sterile patch tester (diameter 8 mm). The blank patch tester is used as a control.

[0065] Application site: inner forearm of the subject. The patch was removed after 24 hours. Skin reaction was observed at 0.5h, 24h and 48h after removal and scored according to the "Cosmetic Skin Irritation Test Method". (0 = no reaction, 1 = slight redness, 2 = obvious redness, 3 = redness and swelling, 4 = redness and swelling + blisters).

[0066] The test results are shown in the table below:

[0067] Patch Test Form No significant skin irritation was observed in any of the tested samples during the 48-hour observation period (average score ≤0.1), and the incidence of allergies was 0, demonstrating that the formula is mild and safe.

[0068] The nano-emulsifier used in Example 1 has a similar structure to the skin barrier and is highly skin-friendly. Furthermore, the fermented jasmine flower extract has had plant-derived impurities removed after fermentation with lactic acid bacteria, reducing the risk of allergies and making it especially suitable for sensitive skin.

[0069] Test 2: Tyrosinase Inhibition Assay A 60 U / mL tyrosinase solution was prepared using pH 6.8 and 50 mmol / L PBS buffer, and an 8 g / L composition solution was prepared using 40% ethanol aqueous solution. 40 μL of 0.1 mg / mL tyrosine, 80 μL of PBS buffer, 50 μL of the composition solution, and 50 μL of tyrosinase solution were added sequentially to each well of a 96-well plate. The plates were incubated at 37°C for 30 min, and the absorbance was measured at 475 nm using a microplate reader. Three replicates were performed per group. The calculation formula is as follows: Inhibition rate (%) = [(A1-A2)-(A3-A4)] / (A1-A2)×100% (A1 = absorbance of blank group after incubation, A2 = absorbance of blank group before incubation, A3 = absorbance of sample group after incubation, A4 = absorbance of sample group before incubation) The test results are shown in the table below:

[0070] Statistical table of results of tyrosinase activity inhibition test The tyrosinase inhibition rate of Example 1 reached 83.7%, the highest among all samples, with a standard deviation of only 1.25, demonstrating excellent data stability. This was primarily due to the dual empowerment of nanoemulsification technology and composite active ingredients: the nanoemulsifier controlled the emulsion particle size to 256.56 nm, with a PDI of only 15.75%, significantly improving the dispersion uniformity of fermented jasmine flower extract, arbutin, and tranexamic acid, allowing the active ingredients to fully contact the active sites of tyrosinase. The three components work synergistically across multiple targets: fermented jasmine flower extract reduces tyrosinase production at the source, arbutin directly inhibits tyrosinase activity, and tranexamic acid regulates downstream pathways, thus enhancing the inhibitory effect throughout the entire process.

[0071] The inhibition rates of Comparative Example 2 and Comparative Example 3 were 81.56% and 81.49%, respectively. Due to the use of nanoemulsion technology, they still maintained a high inhibition rate. However, the lack of a single core component led to the partial breakage of the synergistic link, and the effect was slightly lower than that of Example 1. Comparative Example 1 (without arbutin and tranexamic acid) relied solely on fermented jasmine flower extract, resulting in a limited whitening pathway and a reduction in inhibition rate to 80.31%, highlighting the synergistic value of the three ingredients combined.

[0072] Example 2 (ordinary hydrogenated lecithin) had an inhibition rate of 75.81%, which was the lowest among the samples containing core active ingredients. The key reason is that the ordinary emulsifier used could not form a nanoscale dispersion, and the active ingredients had insufficient contact with tyrosinase. Even if the three core ingredients were retained, the effect was reduced due to the limited efficiency of action.

[0073] The inhibition rate of the blank sample was 30.45%, which was much lower than that of all samples containing the core active ingredient. This eliminated the non-specific influence of the basic formulation and proved that the high inhibition rate of each sample was due to the effect of the core active ingredient and the optimization of the nanoemulsion technology. This fully reflects the core advantages of the nanoemulsion system and composite component design of this patent.

[0074] Test 3: Human Skin Whitening Efficacy Test Testing Method: Sixty healthy subjects (aged 25-55) were selected and randomly divided into 6 groups (20 people in each group). The initial ITA° value was detected using the VISIA facial image analysis system and Image-ProPlus image analysis software (the higher the ITA° value, the brighter the skin tone). After detecting the initial ITA° value, each consumer was required to use the serum formula in Table 2 every morning and evening after cleansing for 1 month. The final ITA° value was then measured, and the calculation formula is as follows: ITA° value change rate (%) = (final ITA° value - initial ITA° value) / initial ITA° value × 100% The test results are shown in the table below:

[0075] Human Skin Whitening Test Results - ITA° Value Statistics Table The ITA° value change rate of Example 1 reached 25.0%, which was significantly better than all other samples. The core reason for this was the synergistic effect of nanoemulsification technology and compound active ingredients: the nanoemulsifier controlled the emulsion particle size at 256.56nm, which greatly improved the transdermal absorption efficiency of fermented jasmine flower extract, arbutin and tranexamic acid. The three respectively cover the upstream regulation of melanin production, the midstream enzyme activity inhibition and the downstream transport blockage, forming a complete whitening pathway.

[0076] The change rates of Comparative Example 2 (without arbutin) and Comparative Example 3 (without tranexamic acid) were 16.2% and 16.2%, respectively. The effects were similar and significantly better than those of Comparative Example 1. This indicates that the absence of a single core ingredient only leads to the disruption of a part of the whitening pathway. It can still exert a synergistic effect through "fermented jasmine flower extract and arbutin / tranexamic acid". However, Comparative Example 1 (without arbutin and tranexamic acid) only retains fermented jasmine flower extract, and the whitening pathway is singular, so the change rate dropped to 14.5%.

[0077] The change rate of Example 2 (ordinary hydrogenated lecithin) was 13.5%, slightly lower than that of Comparative Example 1. The key reason is that the ordinary hydrogenated lecithin (phosphatidylcholine content of about 25%) used in it cannot form a nano-scale emulsion, and the active ingredients have difficulty penetrating the stratum corneum of the skin. Even if the three core ingredients are retained, the effect is limited due to insufficient absorption efficiency.

[0078] The change rate of the blank sample was only 4.39%, which only reflects the skin brightening effect of natural skin metabolism. This eliminated the interference of inactive ingredients on the test results and further verified that the whitening effect of each sample was due to the action of the core active ingredients and the optimization of emulsification technology, fully demonstrating the core advantages of this patented composition: "highly effective whitening + gentle and safe".

[0079] The nanoemulsification technology of this patent (selection of nanoemulsifier and process optimization) is the key to improving the whitening effect: Example 1 improves the absorption efficiency of active ingredients through nanoemulsification technology, and combined with the multi-target synergy of "fermented jasmine flower extract + arbutin + tranexamic acid", the best whitening effect is achieved (ITA° increased by 25.0%). The synergistic effect of the compound active ingredients is indispensable: the three core ingredients cover the upstream, midstream and downstream pathways of melanin production, respectively. The absence of a single or dual ingredient will lead to a significant decrease in the effect, which verifies the innovation of the compound system. The type of emulsifier directly affects efficacy: ordinary hydrogenated lecithin cannot form a nano-emulsion, resulting in insufficient absorption of active ingredients and its effect is far inferior to the nano-emulsifiers (hydrogenated lecithin and phytosterols) selected in the patent. All samples containing the core active ingredient showed significantly better results than the blank sample, with a smaller standard deviation, proving that the whitening effect of this patented composition is stable and reliable, and meets the requirements for human efficacy evaluation of cosmetics.

[0080] The composition of the present invention may optionally contain commonly used ingredients in cosmetic compositions, including skin conditioning agents, emollients, polyols, active ingredients in skin care products, and cosmetic excipients, and their types and amounts can be selected according to specific needs. In operation, fermented jasmine flower extract, arbutin, and tranexamic acid are emulsified into microparticles by nanoemulsification technology with optimized formulation and process, under the emulsification action of nanoemulsifiers, to achieve a highly efficient whitening, spot-fading, and repairing composition, and then added to the formula.

[0081] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A whitening and spot-fading composition based on fermented jasmine flower extract, characterized in that, It includes the following components in parts by weight: 0.1-1 parts nano-emulsifier, 0.5-8 parts fermented jasmine flower extract, 0.5-1.5 parts arbutin, and 1-3 parts tranexamic acid.

2. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 1, characterized in that: The nanoemulsifier is a mixture of hydrogenated lecithin and phytosterols, with a mass fraction ratio of arbutin to tranexamic acid of 0.5:

1.

3. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 1, characterized in that: The fermented jasmine flower extract is specifically composed of: jasmine flower extract, lactic acid bacteria fermentation extract, water, 1,2-hexanediol, and 1,2-pentanediol.

4. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 3, characterized in that: The fermented jasmine flower extract contains ≥3% jasmine flower extract.

5. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 1, characterized in that: The ratio of the nanoemulsifier to the fermented jasmine flower extract is (0.1-1):

1.

6. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 1, characterized in that: The ratio of fermented jasmine flower extract to arbutin is (0.5-3):

1.

7. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 1, characterized in that: The components include the following parts by weight: 0.05-0.2 parts chelating agent; 0.15-1 part thickener; 0.1-5 parts humectant; 0.1-5 parts preservative; 5-20 parts polyols; 2-20 parts moisturizer; 0.5-10 parts skin conditioning agent; 0.1-5 parts antioxidant; 0.1-2 parts pH adjuster; Water is added to the remaining amount.

8. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 7, characterized in that: The polyol is selected from any one or at least a combination of two of the following: glycerol, butanediol, 1,3-propanediol, propylene glycol, dipropylene glycol, methylpropanediol, 1,2-pentanediol, and 1,2-hexanediol.

9. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 8, characterized in that: The polyol content is 5-20 parts by mass, and the mass percentage of polyol and nanoemulsifier is (5-20):

1.

10. The whitening and spot-fading composition based on fermented jasmine flower extract as described in claim 5, characterized in that: The composition was prepared using nanoemulsification technology, and the pH of the composition was 5.0-6.5.

Images