A DNA hydrogel with a kissing hairpin cross-linking structure, its preparation method, and its application.

By designing a DNA hydrogel with a kiss-like hairpin cross-linking structure, the problem of rapid decomposition of existing DNA hydrogels in vivo has been solved, achieving controllable drug release and safe and efficient TNF-α inhibition, which has the effect of treating rheumatoid arthritis.

CN121550145BActive Publication Date: 2026-05-26ANHUI MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI MEDICAL UNIV
Filing Date
2026-01-22
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

The existing DNA hydrogels that deliver nucleic acid aptamers have excessively strong cross-linking strength, making it difficult for them to rapidly decompose into monomers or oligomers in vivo to exert their effects. This results in delayed drug release, and traditional TNF-α inhibitors also have side effects and high costs.

Method used

A DNA hydrogel employing a kissing hairpin cross-linking structure is designed to bind to a nucleic acid aptamer that specifically recognizes TNF-α, forming an injectable hydrogel that can rapidly respond to changes in the physiological environment, thus achieving controlled and sustained drug release.

Benefits of technology

It achieves rapid response to environmental changes in vivo, with high safety and low cost, drug sustained release, significantly reduces TNF-α levels, and effectively treats rheumatoid arthritis.

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Abstract

This invention belongs to the field of biomedical technology, specifically relating to a DNA hydrogel with a kissing hairpin cross-linking structure, its preparation method, and its applications. The first end of the DNA single strand is connected to a first sticky end carrying a nucleic acid aptamer that specifically recognizes TNF-α, and the second end is connected to a second sticky end. The two hairpin structures form an integrated hairpin structure through complementary base pairing at their sticky ends. The loop regions of multiple integrated hairpin structures are cross-linked through complementary base pairing to form a DNA hydrogel with a three-dimensional network backbone. This application utilizes the self-assembly of anti-TNF-α nucleic acid aptamers to form a hydrogel via DNA kissing hairpin cross-linking, achieving controllable drug release. Compared to various TNF-α monoclonal antibodies and fusion protein biopharmaceuticals, anti-TNF-α nucleic acid aptamers offer the advantages of both good targeting and low cost; the self-assembly of the hydrogel via DNA kissing hairpin cross-linking allows for a wider range of adjustable release and higher safety.
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