Preparation method of antibacterial peptide type amino acid organic water-soluble fertilizer for leaf surfaces

Abstract

The invention discloses a preparation method of an antibacterial peptide type amino acid organic water-soluble fertilizer for leaf surfaces, which comprises the following steps: carrying out salt-reducing antioxidant compounding on amino acid mother liquor and animal blood enzymatic hydrolysate to obtain a solution A, adsorbing, coating and drying bacillus subtilis fermentation liquor to prepare powder B, and preparing the solution A and the powder B when the solution A and the powder B are used to obtain the organic water-soluble fertilizer for leaf surface spraying. Nutrition supply and disease prevention and control are synchronously realized, the chlorophyll content of plants is increased, and the bottlenecks of low amino acid activity, nutrient antagonism, single function, easy inactivation of bioactive components and the like of existing products are solved.

Description

Technical Field

[0001] This invention relates to the field of foliar organic water-soluble fertilizer technology, specifically a method for preparing foliar antibacterial peptide-type amino acid organic water-soluble fertilizer. Background Technology

[0002] Foliar fertilizer is a fertilizer in which nutrients are applied to the surface of crop leaves and absorbed by the leaves to exert their functions. It is a supplement to make up for insufficient nutrient absorption by the root system and has the advantages of rapid absorption, strong effect, small dosage and high efficiency.

[0003] Foliar fertilizers come in a wide variety of types, broadly categorized as water-soluble macro- and micro-element foliar fertilizers, organic humic acid foliar fertilizers, microbial foliar fertilizers, natural mineral foliar fertilizers, plant stimulant foliar fertilizers, and pesticide-based foliar fertilizers. Different types of foliar fertilizers have significantly different advantages and disadvantages. For example, the high salt content of traditional chemical fertilizers can easily cause fertilizer damage and disrupt the soil microecology, while the large organic molecules in ordinary organic foliar fertilizers are difficult for leaves to absorb directly, resulting in slow fertilizer effects. Amino acid water-soluble fertilizers, which emerged in the 1990s, provide crops with a large amount of high-quality small-molecule nitrogen, but their nutritional function is limited and they lack the ability to control biological stress (diseases). In 2010, the EU and the US included "peptides" in the category of biostimulants (such as animal blood hydrolysates), promoting the development of stress-resistant and growth-promoting functional fertilizers. Antimicrobial peptides derived from animals, plants, and microorganisms have broad-spectrum antibacterial properties and are not prone to inducing resistance (such as cecropin and defensin), and have good control potential against downy mildew, powdery mildew, bacterial wilt, etc.

[0004] The compound use of foliar fertilizers can promote growth, prevent diseases and increase yield while reducing dosage and saving labor time, which is the direction of foliar fertilizer development. However, the bottleneck of peptide compound technology is mainly manifested in poor stability and single nutritional effects, such as: ultraviolet degradation, hydrolysis by plant epidermal enzymes (proteases), rainwater rinsing, and charge neutralization precipitation after mixing with high-salt amino acid mother liquor. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer. This method simultaneously achieves nutrient supply and disease control, increases plant chlorophyll content, and overcomes the bottlenecks of existing products such as low amino acid activity, nutrient antagonism, limited functionality, and easy inactivation of bioactive components. Furthermore, the separate preparation of solution A and powder B allows for immediate mixing and significantly improves product stability.

[0006] The technical solution adopted by the present invention to solve the above-mentioned technical problems is as follows:

[0007] A method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer includes the following steps:

[0008] Step 1: Preparation of agricultural amino acid stock solution for desalination and diluted amino acid mother liquor;

[0009] Step 2: Dilute animal blood enzymatic hydrolysate for antioxidant purposes;

[0010] Step 3: Prepare foliar amino acid organic water-soluble fertilizer solution A by compounding.

[0011] Step 4: Bacillus subtilis fermentation broth adsorption and coating to prepare B powder.

[0012] Step 5: Mix liquid A and powder B at a ratio of 1L:5-10g, dilute 100-1000 times and spray or drip irrigate.

[0013] In step 1, add an equal volume of saturated lime water to the agricultural amino acid stock solution, stir gently for 30-120 minutes, let stand for 1-4 hours, filter, take the clear liquid, and then adjust the amino acid ratio in the clear liquid with water and glutamic acid so that glutamic acid accounts for 70-80% of the total amino acids, alanine accounts for 12.5-16% of the total amino acids, lysine accounts for 3-5% of the total amino acids, phenylalanine accounts for 2.5-5% of the total amino acids, isoleucine accounts for 1.5-2% of the total amino acids, valine accounts for 0.25-1% of the total amino acids, aspartic acid accounts for 0.2-0.9% of the total amino acids, and other amino acids account for 0.05-0.1% of the total amino acids. Adjust the pH to 6.5-7.5 to form a diluted amino acid stock solution.

[0014] In step 2, water is added to the animal blood enzymatic hydrolysate to dilute it to a crude protein content of 20-40%, small molecule peptides accounting for 50-70% of the total peptides, free amino acids 15-35%, total iron 0.1-1 ug / ml, and B vitamins 0.001-0.1 ug / ml. 0.03-0.8% of antioxidant vitamin C or tea polyphenols are added to the diluent, and the pH of the diluent is adjusted to 6.5-7.5 using KOH or ethanolamine.

[0015] In step 3, using the above-mentioned amino acid mother liquor dilution as a solvent and nutrient base, the above-mentioned animal blood enzymatic hydrolysate dilution is added at a volume ratio of (3-10):3 to form a compound solution. The mixture is stirred at 50 rpm for 30 min. 0.01-0.5% alkyl polysaccharide APG or Tween 80 is added to the compound solution. The compound solution is preheated to 55-60°C using a plate or tubular heat exchanger, and the temperature is precisely controlled to 72°C and maintained for 15-30 seconds. The heat exchanger is then immediately cooled to 25-30°C and bottled as solution A.

[0016] In step 4, bentonite, attapulgite, or diatomaceous earth is pulverized to 200-400 mesh and then subjected to heat expansion treatment in a flash dryer at 300-500℃. The expanded material is then mixed with the fermentation broth, which is a concentrated culture medium. The fermentation broth of antimicrobial peptides from Bacillus subtilis (fermentation preparation method and specific components can be found in the literature Chen Hua et al. Lipopeptide antibiotics produced by Bacillus subtilis JA) Purification and electrospray mass spectrometry identification of Bacillus subtilis JA. Acta Microbiologica Sinica, 2008, 48(1):116–120 and Chen Hua, et al. Study on the antibacterial effects of lipopeptides and volatile substances of Bacillus subtilis JA. Bulletin of Microbiology, 2008, 35(1):1–4)

[0017] The ratio of puffed material to fermentation broth is 10-20 g: 1 L. After thorough stirring, filtration, and solid-phase air drying at room temperature, the mixture is pulverized to 200-400 mesh and added to a chitosan solution at a concentration of 0.5-1.5% w / v. The pH is adjusted to 5.0 with acetic acid or citric acid, and the mixture is stirred for 2-10 min. The fluidized bed dryer is operated at an inlet temperature of 35-40℃ and an air velocity of 1.5 m / s to obtain powder B.

[0018] The beneficial effects that can be achieved by adopting the above-mentioned technical solution in this invention are:

[0019] 1. Pretreatment with a mixture of agricultural amino acid stock solution and lime water to reduce the concentration of amino acid in the mother liquor. To control salt content and prevent high salt poisoning;

[0020] 2. The nutritional composition of the diluted amino acid mother liquor is clearly defined, with the main components including water, glutamic acid, alanine, lysine, phenylalanine, isoleucine, and trace amounts of inorganic salts.

[0021] 3. After dilution, animal blood enzymatic hydrolysate is treated with antioxidant vitamin C or tea polyphenols to prevent heme iron from catalyzing lipid oxidation and producing aldehyde and ketone odors.

[0022] 4. Solution A is prepared by combining amino acid mother liquor dilution and animal blood enzymatic hydrolysate dilution, and nonionic surfactants such as alkyl polysaccharide APG or Tween are added to improve leaf spreading and adhesion.

[0023] 5. After adsorbing the Bacillus subtilis fermentation broth with expanded bentonite, attapulgite, or diatomaceous earth, the mixture is coated with chitosan and dried to obtain powder B. However, the problem of poor product stability arises from the need to prepare solution A and powder B immediately before use.

[0024] 6. Experiments show that the organic water-soluble fertilizer solution A prepared by this method has a uniform appearance, no stratification, and is light yellow to brownish-yellow in color. It has a pH of 6.5-7.5, a density of 1.05-1.15 g / ml, and a water-insoluble content ≤ 0.5%. After 12 months of storage at room temperature (25℃) away from light, it shows no precipitation, stratification, or spoilage. Accelerated testing (40℃, 90 days) shows no abnormalities. The total amino acid content is ≥100 g / L, the organic matter content is ≥100 g / L, small molecule peptides (molecular weight < 1000 Da) account for ≥60% of the total peptide and amino acid content, and the iron content is ≥0.1 mg / L. The bacterial cell encapsulation rate in powder B reaches 85-95%, and the bacterial cell survival rate is 80-90% after 6 months of storage at room temperature, with 60-80% of the antimicrobial peptide activity retained. Attached Figure Description

[0025] Figure 1 This is a flowchart of the present invention. Detailed Implementation

[0026] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in these embodiments.

[0027] It should be noted that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes and to aid those skilled in the art in understanding and reading the invention. They are not intended to limit the scope of the invention and therefore have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to the size are not considered grounds for limitation. The following embodiments are provided to better understand the invention, but are not intended to limit it. Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods. Unless otherwise specified, the experimental materials used in the following embodiments were purchased from conventional biochemical reagent stores.

[0028] Unless otherwise specified, all reagents or materials described in the following examples are commercially available.

[0029] Unless otherwise specified in the technical solution, all concentrations are mass-volume concentrations (w / v).

[0030] In the technical solution, glutamic acid (solid powder, 99.5% purity, packaged in 25 kg packages) is sourced from food additive products of Fufeng Group Co., Ltd., and agricultural amino acid stock solution (main components are glutamic acid, alanine, valine, aspartic acid, etc.) is sourced from Xuzhou Green Source Agricultural Technology Co., Ltd. The amino acid stock solution is prepared by compounding the above-mentioned glutamic acid and agricultural amino acid stock solution. During the preparation process, glutamic acid accounts for 50-70% of the total amino acids, alanine accounts for 1-15% of the total amino acids, lysine accounts for 0.5-6% of the total amino acids, phenylalanine accounts for 0.5-4% of the total amino acids, isoleucine accounts for 0.1-3% of the total amino acids, valine accounts for 0.1-1% of the total amino acids, aspartic acid accounts for 0.2-0.9% of the total amino acids, and other amino acids account for 0.01-0.1% of the total amino acids.

[0031] The animal blood enzymatic hydrolysate in the technical solution uses edible hydrolyzed protein peptide products from Xiangyang Weien Biotechnology Co., Ltd.

[0032] Example 1: A method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer, comprising the following steps:

[0033] Step 1: Add an equal volume of saturated lime water to 1L of agricultural amino acid stock solution, stir gently for 30 minutes, let stand for 1 hour, filter, take the clear liquid, add water and stir to dilute, adjust the proportion of glutamic acid to 70% of the total amino acids, alanine to 16% of the total amino acids, lysine to 5% of the total amino acids, phenylalanine to 5% of the total amino acids, isoleucine to 2% of the total amino acids, valine to 1% of the total amino acids, aspartic acid to 0.9% of the total amino acids, and other amino acids to 0.1% of the total amino acids with water and glutamic acid, adjust the pH to 6.5 with KOH to obtain the diluted amino acid stock solution;

[0034] Step 2: Dilute 1L of animal blood enzymatic hydrolysate with water to a crude protein content of approximately 20%, small molecule peptides (molecular weight <1000 Da) of 50-55% (accounting for the total peptide content), free amino acids of 15-20%, total iron of 0.1 ug / ml, and B vitamins of 0.001 ug / ml. Add 0.03% vitamin C to the diluent and adjust the pH of the diluent to 7.5 with KOH.

[0035] Step 3: Take the diluted amino acid mother liquor and add the diluted animal blood enzymatic hydrolysate at a volume ratio of 1:1. Stir at 50 rpm for 30 min to form a compound solution. Add 0.01% alkyl polysaccharide APG to the compound solution. Preheat the compound solution to 55°C using a plate or tubular heat exchanger, precisely control the temperature to 72°C, hold for 15 seconds, and immediately cool it to 25°C using a heat exchanger. Bottle it as solution A.

[0036] Solution A is yellowish-brown, pH 7.3, density 1.08 g / ml, water-insoluble content 0.4%, and after 12 months of storage at room temperature (25℃) protected from light, it showed no precipitation, stratification, or spoilage. Accelerated testing (40℃, 90 days) showed no abnormalities. The total amino acid content is 103 g / L, the organic matter content is 119 g / L, small molecule peptides (molecular weight < 1000 Da) account for 65% of the total peptides and amino acids, and the iron content is 0.7 mg / L.

[0037] Step 4: Crush bentonite to 400 mesh, heat-expand at 400℃, and then add the cooled bentonite powder to the bacterial concentrate. In the Bacillus subtilis fermentation broth, the mixing ratio was 10g:1L. After stirring thoroughly for 60 min, the mixture was filtered, air-dried at room temperature, and then pulverized to 400 mesh. The pulverized mixture was added to a chitosan solution at a concentration of 0.5% w / v. The pH was adjusted to 5.0 with acetic acid and stirred for 2 min. The mixture was then dried in a fluidized bed dryer at an inlet temperature of 35℃ and an air velocity of 1.5 m / s to obtain powder B.

[0038] The bacterial cell encapsulation rate of B powder reaches 88%, the bacterial cell survival rate is 84% ​​after 6 months of storage at room temperature, and the antimicrobial peptide activity is retained at 69%.

[0039] Mix 1 L of solution A with 5 g of powder B, then dilute 100 times to form an organic water-soluble fertilizer.

[0040] The effect of this organic water-soluble fertilizer on the biomass of seed maize variety FL-52 was investigated. The application scheme and comparative data are shown in Table 1 below:

[0041] (1) A commercially available brand of amino acid water-soluble fertilizer and this product were compared, with other fertilization methods remaining the same;

[0042] (2) Foliar spraying three times: seedling stage (4 leaves), small trumpet stage (8 leaves), and large trumpet stage (12 leaves).

[0043] (3) Measurements were taken one week after the female parent was emasculated. The unit of measurement was centimeters (cm), and the measured data were plant height and ear height.

[0044] Table 1 compares this product with a commercially available brand of amino acid water-soluble fertilizer.

[0045]

[0046] Example 2:

[0047] Step 1: Add an equal volume of saturated lime water to 1L of agricultural amino acid stock solution, stir gently for 1 hour, let stand for 3 hours, filter, take the clear liquid, add water and stir to dilute, adjust the proportion of glutamic acid to 80% of the total amino acids, alanine to 12.5% ​​of the total amino acids, lysine to 3% of the total amino acids, phenylalanine to 2.5% of the total amino acids, isoleucine to 1.5% of the total amino acids, valine to 0.25% of the total amino acids, aspartic acid to 0.2% of the total amino acids, and other amino acids to 0.05% of the total amino acids with water and glutamic acid. Adjust the pH to 7.2 with ethanolamine to obtain the diluted amino acid stock solution.

[0048] Step 2: Dilute 1L of animal blood enzyme peptide hydrolysate with water to a crude protein content of approximately 30%, small molecule peptides (molecular weight <1000 Da) of 55-60% (accounting for the total peptide content), free amino acids of 18-23%, total iron of 0.6 ug / ml, and B vitamins of 0.05 ug / ml. Add 0.5% tea polyphenols to the diluent and adjust the pH of the diluent to 7.5 using ethanolamine.

[0049] Step 3: Take the above amino acid mother liquor dilution and add the above animal blood enzymatic hydrolysate dilution at a volume ratio of 10:3 to form a compound solution. Stir at 50 rpm for 30 min. Add 0.3% Tween 80 to the compound solution. Preheat the compound solution to 55°C using a plate or tubular heat exchanger, precisely control the temperature to 72°C, hold for 20 seconds, and immediately cool it to 30°C using a heat exchanger. Bottle it as solution A.

[0050] Solution A is dark yellowish-brown, pH 7.5, density 1.11 g / ml, water-insoluble matter content 0.4%, and after 12 months of storage at room temperature (25℃) protected from light, it showed no precipitation, stratification, or spoilage. Accelerated testing (40℃, 90 days) showed no abnormalities. The total amino acid content is 109 g / L, the organic matter content is 121 g / L, small molecule peptides (molecular weight < 1000 Da) account for 67% of the total peptides and amino acids, and the iron content is 1.1 mg / L.

[0051] Step 4: Crush the attapulgite soil to 400 mesh, perform thermal expansion treatment at 500℃, and then add the cooled attapulgite soil powder to the bacterial concentrate. In the Bacillus subtilis fermentation broth, the mixing ratio was 15g:1L. After stirring thoroughly for 60 min, the mixture was filtered, air-dried at room temperature, and then pulverized to 300 mesh. The pulverized mixture was added to a chitosan solution at a concentration of 1% w / v. The pH was adjusted to 5.0 with citric acid, and the mixture was stirred for 5 min. The mixture was then dried in a fluidized bed dryer at an inlet temperature of 40℃ and an air velocity of 1.5m / s to obtain powder B.

[0052] The bacterial cell encapsulation rate of B powder reaches 85%, the bacterial cell survival rate is 86% after 6 months of storage at room temperature, and the antimicrobial peptide activity is retained at 79%.

[0053] Step 5: Mix 1 L of solution A with 8 g of powder B, and dilute 100 times to form an organic water-soluble fertilizer.

[0054] The effect of this organic water-soluble fertilizer on the photosynthetic capacity of the seed maize variety FL-52 was investigated by spraying it. The application scheme and comparative data are shown in Table 2 below:

[0055] (1) A commercially available brand of amino acid water-soluble fertilizer and this product were compared, with other fertilization methods remaining the same;

[0056] (2) Foliar spraying three times: seedling stage (4 leaves), small trumpet stage (8 leaves) and large trumpet stage (12 leaves).

[0057] (3) Measure one week after the female parent is emasculated. The unit of measurement is centimeters (cm). Measure the length, maximum width and thickness of the leaves at the ear position;

[0058] Table 2 compares this product with a commercially available brand of amino acid water-soluble fertilizer.

[0059]

[0060] Example 3:

[0061] Step 1: Add an equal volume of saturated lime water to 1L of agricultural amino acid stock solution, stir gently for 2 hours, let stand for 4 hours, filter, take the clear liquid, add water and stir to dilute, adjust the proportion of glutamic acid to 76% of the total amino acids, alanine to 14% of the total amino acids, lysine to 3.5% of the total amino acids, phenylalanine to 3.5% of the total amino acids, isoleucine to 1.75% of the total amino acids, valine to 0.75% of the total amino acids, aspartic acid to 0.45% of the total amino acids, and other amino acids to 0.05% of the total amino acids with water and glutamic acid. Adjust the pH to 7.5 with citric acid to obtain the diluted amino acid stock solution.

[0062] Step 2: Add water to 1L of animal blood enzymatic hydrolysate to dilute to a crude protein content of approximately 40%, small molecule peptides (molecular weight <1000 Da) of 65-70% (accounting for the total peptide content), free amino acids of 30-35%, total iron of 1 ug / ml, and B vitamins of 0.1 ug / ml. Add 0.8% antioxidant vitamin C to the diluent and adjust the pH of the diluent to 6.5 using KOH.

[0063] Step 3: Take the above amino acid mother liquor dilution and add the above animal blood enzymatic hydrolysis dilution at a volume ratio of 2:1 to form a compound solution. Stir at 50 rpm for 30 min. Add 0.5% Tween 80 to the compound solution. Preheat the compound solution to 60°C using a plate or tubular heat exchanger, precisely control the temperature to 72°C, hold for 20 seconds, and immediately cool it to 30°C using a heat exchanger. Bottle it as solution A.

[0064] Solution A is dark brown, pH 7.5, density 1.13 g / ml, water-insoluble content 0.4%, and after 12 months of storage at room temperature (25℃) protected from light, it showed no precipitation, stratification, or spoilage. Accelerated testing (40℃, 90 days) showed no abnormalities. The total amino acid content is 111 g / L, the organic matter content is 125 g / L, small molecule peptides (molecular weight < 1000 Da) account for 70% of the total peptides and amino acids, and the iron content is 1.4 mg / L.

[0065] Step 4: Crush the diatomaceous earth to 200 mesh, perform heat expansion treatment at 300℃, and then add the cooled diatomaceous earth powder to the bacterial concentrate. In the Bacillus subtilis fermentation broth, the mixing ratio was 20 g: 1 L. After stirring thoroughly for 60 min, the mixture was filtered, air-dried naturally at room temperature, pulverized to 200 mesh, and added to a chitosan solution at a concentration of 1.5% w / v. The pH was adjusted to 5.0 with acetic acid, and the mixture was stirred for 10 min. The fluidized bed dryer was operated at an inlet temperature of 40℃ and an air velocity of 1.5 m / s to obtain powder B.

[0066] The bacterial cell encapsulation rate of B powder reaches 95%, the bacterial cell survival rate is 90% after 6 months of storage at room temperature, and the antimicrobial peptide activity is retained by 80%.

[0067] Step 5: Mix 1 L of solution A with 10 g of powder B, and dilute 100 times to form an organic water-soluble fertilizer.

[0068] The effect of this organic water-soluble fertilizer on the thousand-grain weight of seed-producing maize variety FL-52 was investigated. The application scheme and comparative data are shown in Table 3 below.

[0069] (1) A commercially available brand of amino acid water-soluble fertilizer and this product were compared, with other fertilization methods remaining the same;

[0070] (2) Foliar spraying three times: seedling stage (4 leaves), small trumpet stage (8 leaves) and large trumpet stage (12 leaves).

[0071] (3) After harvesting, three bases were randomly selected with two types of fertilization. After drying, the weight of 1,000 seeds was measured to examine the basic quality of the seeds.

[0072] Table 3 compares this product with a commercially available brand of amino acid water-soluble fertilizer.

[0073]

Claims

1. A method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer, characterized in that, Includes the following steps: Step 1: Preparation of agricultural amino acid stock solution for desalination and diluted amino acid mother liquor; Step 2: Dilute animal blood enzymatic hydrolysate for antioxidant purposes; Step 3: Prepare foliar amino acid organic water-soluble fertilizer solution A by compounding; Step 4: Bacillus subtilis fermentation broth adsorption and coating to prepare B powder; Step 5: Mix liquid A and powder B at a ratio of 1L:5-10g, and dilute 100 times to form an organic water-soluble fertilizer.

2. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 1, characterized in that: In step 1, add an equal volume of saturated lime water to the agricultural amino acid stock solution, stir gently, let stand, filter, and take the clear liquid. Then, adjust the amino acid ratio in the clear liquid with water and glutamic acid so that glutamic acid accounts for 70-80% of the total amino acids, alanine accounts for 12.5-16% of the total amino acids, lysine accounts for 3-5% of the total amino acids, phenylalanine accounts for 2.5-5% of the total amino acids, isoleucine accounts for 1.5-2% of the total amino acids, valine accounts for 0.25-1% of the total amino acids, aspartic acid accounts for 0.2-0.9% of the total amino acids, and other amino acids account for 0.05-0.1% of the total amino acids. Adjust the pH to 6.5-7.5 to form a diluted amino acid stock solution.

3. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 2, characterized in that: In step 1, stir gently for 30-120 minutes, let stand for 1-4 hours, and adjust the pH value with KOH, ethanolamine, or citric acid.

4. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 1, characterized in that: In step 2, water is added to the animal blood enzymatic hydrolysate to dilute it to a crude protein content of 20-40%, small molecule peptides accounting for 50-70% of the total peptides, free amino acids 15-35%, total iron 0.1-1 ug / ml, B vitamins 0.001-0.1 ug / ml, and 0.03-0.8% antioxidant is added to the diluent. The pH of the diluent is adjusted to 6.5-7.

5.

5. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 4, characterized in that: In step 2, the antioxidant is vitamin C or tea polyphenols, and the pH value is adjusted using KOH or ethanolamine.

6. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 1, characterized in that: In step 3, the above-mentioned amino acid mother liquor dilution is used as a solvent and nutrient base, and the above-mentioned animal blood enzymatic hydrolysate dilution is added at a volume ratio of (3-10):3 to form a compound solution. The solution is stirred at 50 rpm for 30 min, and alkyl polysaccharide APG or Tween 80 is added to the compound solution. The compound solution is preheated by a plate or tubular heat exchanger, and the heat exchanger is immediately cooled and bottled as solution A.

7. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 6, characterized in that: In step 3, the added alkyl polysaccharide APG or Tween 80 is 0.01-0.5%.

8. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 6, characterized in that: In step 3, the compound solution is preheated to 55-60°C by a plate or tubular heat exchanger, precisely controlled to 72°C, held for 15-30 seconds, and then immediately cooled to 25-30°C before being bottled as solution A.

9. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 1, characterized in that: In step 4, bentonite, attapulgite, or diatomaceous earth is pulverized to 200-400 mesh and subjected to thermal expansion treatment in a flash dryer at 300-500℃. The expanded material is mixed with the fermentation broth, stirred thoroughly, filtered, and allowed to air dry naturally at room temperature. After pulverizing to 200-400 mesh, it is added to the chitosan solution and stirred for 2-10 minutes. The fluidized bed dryer is operated at an inlet temperature of 35-40℃ and an air velocity of 1.5 m / s to obtain powder B.

10. The method for preparing a foliar antibacterial peptide-type amino acid organic water-soluble fertilizer according to claim 9, characterized in that: In step 4, the fermentation broth is a concentrated bacterial culture. The fermentation broth of Bacillus subtilis was prepared by mixing puffed material and fermentation broth at a ratio of 10-20 g: 1 L, adding chitosan solution at 0.5-1.5% w / v, and adjusting the pH to 5.0 with acetic acid or citric acid.

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