Living germplasm conservation device for breeding northeast wood frogs

Abstract

The invention discloses a germplasm preservation living body conservation device for northeast wood frog breeding, which relates to the technical field of aquatic product breeding and cultivation and comprises a semi-embedded breeding box, a partition plate, a breeding platform, a wood frog hibernation pipe, a circulating water pumping hose and a circulating water return pipe. The semi-embedded breeding box is adopted as a main body structure for wood frog overwintering and breed conservation, multiple sets of wood frog hibernation pipes are arranged in the semi-embedded breeding box to serve as hibernation sheltering points of wood frogs, the problem that wood frogs are stacked and interfere with one another in a traditional overwintering pond is effectively solved, the semi-embedded breeding box can be rapidly set up and put into use, and the survival rate of the wood frogs is increased. The wood frog hibernation shelter can be rapidly arranged in a wood frog breeding site, the main flow guide pipe is arranged in the wood frog hibernation pipe, a slow flow channel for water circulation is formed between the main flow guide pipe and the wood frog hibernation pipe, and hibernation of wood frogs is not affected while it is guaranteed that water at the wood frog hibernation shelter is fresh through water circulation; the risk of local oxygen deficit of the wood frogs is effectively reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of aquaculture, in particular to a kind of gynaecium preservation live body seed storage device for breeding of Northeast forest frog. BACKGROUND

[0002] Northeast forest frog is an important ecological and economic species in China, and the live body preservation of its germplasm is the basis for maintaining population reproduction and ensuring the sustainable development of related industries. Overwintering seed preservation is the most technically difficult and risky core link in this process. In the natural state, Northeast forest frog hides in deep water areas that do not freeze in autumn and hibernates in underwater stones, fallen leaves or silt. This process is crucial for the development of its gonads and reproduction the following year.

[0003] Currently, overwintering artificial seed preservation mainly uses outdoor soil pools or indoor cement pools to simulate natural water bodies. However, such traditional methods have the problem of uncontrollable overwintering environment. Outdoor water pools are easily affected by extreme weather and have the risk of freezing through. Indoor water pools have high energy consumption and are difficult to maintain a stable low-temperature water environment. Moreover, the survival conditions of outdoor wild water pools are poor. Forest frogs accumulate and hide in open water areas, which easily causes mutual interference and extrusion. Local water bodies are prone to oxygen deficiency and waste accumulation, leading to increased mortality.

[0004] Therefore, there is an urgent need for an integrated seed preservation device that can provide a stable, safe and controllable overwintering environment for Northeast forest frog and efficiently connect with the reproduction process. SUMMARY

[0005] The purpose of the present application is to provide a gynaecium preservation live body seed storage device for breeding of Northeast forest frog to solve the problems raised in the background.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solution: a gynaecium preservation live body seed storage device for breeding of Northeast forest frog, comprising: a semi-buried breeding box with a heat preservation inner cavity inside; a partition plate fixedly installed at the inner top end of the semi-buried breeding box; a breeding platform slidably installed inside the semi-buried breeding box and located on one side of the partition plate, the breeding platform is provided with a honeycomb structure and an upper surface provided with a cobblestone cushion layer, and a hatching slope is fixedly installed at one end of the breeding platform close to the partition plate; a plurality of forest frog hibernation tubes are vertically installed above the breeding platform, a plurality of forest frog hibernation cabins are fixedly installed on the outer side wall of the forest frog hibernation tube, a water passing hole is provided through the inner side wall of the forest frog hibernation cabin, and the water passing hole extends to the inside of the forest frog hibernation tube; A circulating water suction hose is located below the breeding platform and connected to the bottom end of the wood frog hibernation pipe, and a circulating water return pipe is located on the side of the partition away from the breeding platform and penetrates the partition at the bottom end, and a silent circulating pump is provided between the top end of the circulating water return pipe and the top end of the circulating water suction hose.

[0007] Preferably, a main flow guide pipe is fixedly installed inside the wood frog hibernation pipe, and an upper flow guide cover and a lower flow guide cover are fixedly installed at both ends of the main flow guide pipe, respectively, and the upper flow guide cover and the lower flow guide cover are located at both ends of the inside of the wood frog hibernation pipe, and a flow guide inner cavity for guiding water to pass through the wood frog hibernation cabin is formed between the upper flow guide cover, the lower flow guide cover, the main flow guide pipe and the wood frog hibernation pipe. A plurality of flow guide through slots are provided on the outside of the main flow guide pipe and are staggered with the plurality of wood frog hibernation cabins.

[0008] Preferably, a main water return pipe and a main water suction pipe are provided, the main water return pipe is fixedly installed at the output end of the silent circulating pump and connected to the top end of the circulating water return pipe, and the main water suction pipe is fixedly installed at the input end of the silent circulating pump and connected to the top end of the circulating water suction hose. An oxygen supplement pipe is fixedly installed in the middle of the main water return pipe and has an inner diameter smaller than that of the main water return pipe, and a valve is installed between the oxygen supplement pipe and the main water return pipe.

[0009] Preferably, a water return flow guide cover is fixedly installed in the middle of the side of the partition close to the breeding platform, the water return flow guide cover is provided in an arc shape with an opening downward, and the bottom end of the circulating water return pipe is located inside the water return flow guide cover.

[0010] Preferably, a plurality of guide sliding sleeves are fixedly installed on the upper surface of the breeding platform, and a plurality of guide rods are fixedly installed inside the semi-buried breeding box, and the plurality of guide rods are slidably installed inside the plurality of guide sliding sleeves. A winch is fixedly installed at the top end of the semi-buried breeding box and fixedly connected to the upper surface of the breeding platform at the input end, the winch is used to provide power for adjusting the height of the breeding platform, and the breeding platform is adjusted to corresponding heights during the entry of the wood frog into the breeding period, the hibernation period and the exit from the hibernation period.

[0011] Preferably, a ceiling is fixedly installed above the breeding platform, and a plurality of support rods are installed between the lower surface of the ceiling and the upper surface of the breeding platform. An entrance and exit is fixedly installed at the top end of the semi-buried breeding box and located between the ceiling and the breeding platform, and the entrance and exit is used for the wood frog to enter and exit.

[0012] Preferably, an air exchange cover is fixedly installed on the upper surface of the ceiling. The air exchange net is arranged in a frame structure and is fixedly installed between the air exchange cover and the ceiling, and is used to achieve air exchange of the semi-buried breeding box after the ceiling and the entrance are attached.

[0013] Preferably, an outer inspection door is arranged at the top end of the outer side wall of the semi-buried breeding box, and the water state between the partition and the semi-buried breeding box can be inspected after the outer inspection door is opened. An inner inspection door is arranged at the top end of the partition, and the ice surface state above the breeding platform can be inspected after the inner inspection door is opened.

[0014] Preferably, the wood frog hibernation cabin is arranged in a cup-shaped structure.

[0015] Preferably, a drain pipe is fixedly installed at the output end of the mute circulating pump.

[0016] Technical effects and advantages of the present application: 1. The semi-buried breeding box is used as the main structure of the wood frog overwintering and seed preservation, a plurality of wood frog hibernation pipes are arranged in the semi-buried breeding box as hibernation shelters for the wood frogs, the problem of mutual interference of the wood frogs in the traditional overwintering pond is effectively solved, the semi-buried breeding box can be quickly built and put into use, and the breeding and breeding site of the wood frogs can be quickly arranged, the main flow pipe is arranged in the wood frog hibernation pipe, and a slow-flow channel for water circulation is formed between the main flow pipe and the wood frog hibernation pipe, which realizes water circulation, ensures the freshness of the water in the hibernation shelter of the wood frogs, does not affect the hibernation of the wood frogs, effectively reduces the risk of local hypoxia of the wood frogs, and thus improves the survival rate of the wood frog overwintering and seed preservation. 2. The breeding platform can move up and down, during the hibernation period of the wood frogs, the breeding platform is lowered to the lowest point, at this time, the wood frog hibernation pipe is located in the deep water area, and the wood frogs hibernate below the ice surface, during the entry and exit periods of the wood frogs, the breeding platform is raised to the middle point, at this time, the wood frog hibernation pipe is located on the water surface, at this time, the wood frogs can smoothly enter and exit the device, during the breeding and hatching period of the wood frogs, the breeding platform is raised to the highest point, at this time, the hatching slope at one end of the breeding platform and the pebble layer above the breeding platform are located on the water surface, at this time, the wood frogs can hatch and grow with the help of the hatching slope, and the up and down of the breeding platform during different breeding periods of the wood frogs can provide an ecological environment that matches the breeding cycle, and further improves the survival rate of the wood frog seed preservation. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present application.

[0018] Figure 2 It is a schematic diagram of the internal structure of the semi-buried breeding box of the present application.

[0019] Figure 3The figure is a structure diagram of the breeding platform of the present application and the wintering tube of the forest frog.

[0020] Figure 4 The figure is a structure diagram of the breeding platform of the present application.

[0021] Figure 5 The figure is a structure diagram of the partition of the present application.

[0022] Figure 6 The figure is a structure diagram of the wintering tube of the forest frog of the present application.

[0023] Figure 7 The figure is a sectional view of the structure of the wintering tube of the forest frog of the present application.

[0024] Figure 8 The figure is a structure diagram of the Figure 7 The figure is an enlarged schematic diagram of the result at A in the present application.

[0025] Figure 9 The figure is a structure diagram of the silent circulating pump of the present application.

[0026] In the figure: 1, semi-buried breeding box; 2, partition; 3, breeding platform; 4, wintering tube of forest frog; 11, outer inspection door; 21, inner inspection door; 31, pebble cushion layer; 32, hatching slope; 33, ceiling; 41, wintering cabin of forest frog; 42, water passage; 43, main guide tube; 44, guide channel; 45, circulating water suction hose; 46, circulating water return pipe; 47, silent circulating pump; 301, guide sleeve; 302, guide rod; 303, winch; 331, air exchange cover; 332, air exchange net; 333, entrance and exit; 34, support rod; 431, upper guide cover; 432, lower guide cover; 433, guide inner cavity; 461, water return guide cover; 471, main water return pipe; 472, main water suction pipe; 473, drain pipe; 474, oxygen supplement pipe. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0028] As Figures 1 to 9 shown, the germplasm preservation living seed device for breeding of the Northeast forest frog provided by the present application is essentially a semi-buried box with integrated structure and switchable functions, which simulates the complete ecological environment for overwintering preservation of the forest frog.

[0029] In terms of specific structural installation, the structural body can be constructed according to the inventive concept of this embodiment. In this embodiment, no special limitations are imposed.

[0030] In this embodiment, a live-culture preservation device for the breeding of Northeast Forest Frog includes: The semi-embedded breeding box 1 has an insulated inner cavity. The semi-embedded breeding box 1 can be modified from a shipping container by adding an insulation and waterproof layer inside the existing container to construct a water-storage and insulation inner cavity. The partition 2 is fixedly installed at the top of the interior of the semi-buried breeding box 1. The partition 2 divides the interior of the semi-buried breeding box 1 into two areas. The larger area is used for the hibernation and breeding of forest frogs, and the smaller area is used for laying water circulation pipes and equipment maintenance. The external inspection door 11 is located at the top of the outer wall of the semi-submerged breeding box 1. After the external inspection door 11 is opened, the water condition between the partition 2 and the semi-submerged breeding box 1 can be checked. The inner inspection door 21 is located at the top of the partition 2. After the inner inspection door 21 is opened, the condition of the ice surface above the breeding platform 3 can be checked. The breeding platform 3 is installed inside the semi-embedded breeding box 1 and located on one side of the partition 2. The breeding platform 3 is designed with a hollow structure and the upper surface is provided with a pebble pad 31. A hatching slope 32 is fixedly installed at one end of the breeding platform 3 near the partition 2. The inside of the breeding platform 3 is provided with a grid plate as the base layer of the pebble pad 31. The upper surface of the hatching slope 32 is covered with aquatic plants or moss to construct a growth beach for forest frogs. The guide sleeve 301 and the guide rod 302 are provided. Multiple guide sleeves 301 are provided and fixedly installed on the upper surface of the breeding platform 3. Multiple guide rods 302 are provided and fixedly installed inside the semi-embedded breeding box 1. The multiple guide rods 302 are slidably installed inside the multiple guide sleeves 301. The winch 303 is fixedly installed at the top of the semi-buried breeding box 1 and its input end is fixedly connected to the upper surface of the breeding platform 3. The winch 303 is used to provide power for adjusting the height of the breeding platform 3. The breeding platform 3 is adjusted to the corresponding height during the hibernation period, hibernation period and emergence period of the forest frog. The canopy 33 is fixedly installed above the breeding platform 3, and multiple support rods 34 are installed between the lower surface of the canopy 33 and the upper surface of the breeding platform 3. The entrance 333 is fixedly installed at the top of the semi-buried breeding box 1 and located between the roof 33 and the breeding platform 3. The entrance 333 is used for the frogs to enter and exit. The upper surface of the entrance 333 is covered with moss to increase the integration of the device with the external environment and reduce the stress response of the frogs when entering and exiting the device. Ventilation hood 331 is fixedly installed on the upper surface of the ceiling 33; The ventilation net 332 is a frame structure and is fixedly installed between the ventilation hood 331 and the roof 33. The ventilation net 332 is used to realize ventilation of the semi-buried breeding box 1 after the roof 33 and the inlet / outlet 333 are attached. The frog hibernation tube 4 has multiple tubes, all of which are vertically installed above the breeding platform 3. Multiple frog hibernation chambers 41 are fixedly installed on the outer wall of the frog hibernation tube 4. The inner wall of the frog hibernation chamber 41 is provided with water passage holes 42, and the water passage holes 42 extend into the interior of the frog hibernation tube 4. The frog hibernation chamber 41 is designed with a cup-shaped structure. Both the frog hibernation tube 4 and the frog hibernation chamber 41 are made of PVC. The frog hibernation tube 4 is a PVC pipe with multiple grooves for the installation of the frog hibernation chamber 41 made by hot pressing on its outer surface. Water passage holes 42 are opened at the bottom of the grooves. The frog hibernation chamber 41 is a prefabricated cup-shaped structure, which is installed in the grooves opened on the outside of the frog hibernation tube 4 by hot melting. The cup-shaped structure of the frog hibernation chamber 41 can better meet the characteristics of the frog that prefers a small environment. The main flow pipe 43 is fixedly installed inside the frog hibernation tube 4. An upper flow guide shroud 431 and a lower flow guide shroud 432 are fixedly installed at both ends of the main flow pipe 43. The upper flow guide shroud 431 and the lower flow guide shroud 432 are located at both ends of the frog hibernation tube 4. The upper flow guide shroud 431, the lower flow guide shroud 432, the main flow pipe 43 and the frog hibernation tube 4 form a flow guide cavity 433 for guiding water through the frog hibernation chamber 41. When the water flows in the main flow pipe 43, the water in the frog hibernation chamber 41 is slowly drawn out through the flow guide cavity 433 to replace the water in the frog hibernation chamber 41. Furthermore, the water turns multiple times when passing through the flow guide cavity 433, which further reduces the flow speed of the water and thus reduces the disturbance to the hibernating frogs. The guide channel 44 is provided in multiple ways and is provided through the outside of the main flow pipe 43, and the multiple guide channels 44 are respectively staggered with multiple frog hibernation chambers 41. The circulating water pumping hose 45 and the circulating water return pipe 46 are located below the breeding platform 3 and connected to the bottom end of the frog hibernation pipe 4. The circulating water return pipe 46 is located on the side of the partition 2 away from the breeding platform 3 and its bottom end penetrates the partition 2. A silent circulating pump 47 is provided between the top end of the circulating water return pipe 46 and the top end of the circulating water pumping hose 45. The main return water pipe 471 and the main pumping water pipe 472 are fixedly installed at the output end of the silent circulation pump 47 and connected to the top end of the circulation return water pipe 46. The main pumping water pipe 472 is fixedly installed at the input end of the silent circulation pump 47 and connected to the top end of the circulation pumping hose 45. The oxygen supply pipe 474 is fixedly installed in the middle of the main return water pipe 471 and its inner diameter is smaller than that of the main return water pipe 471. A valve is installed between the oxygen supply pipe 474 and the main return water pipe 471. The valve can be a manual valve or a solenoid valve. Opening the valve allows the oxygen supply pipe 474 to be connected to the main return water pipe 471. The difference in inner diameter between the oxygen supply pipe 474 and the main return water pipe 471 creates a Venturi effect, which can draw in outside air during water circulation to achieve the effect of oxygenation. The return water guide hood 461 is fixedly installed on the middle part of the side of the partition 2 near the breeding platform 3. The return water guide hood 461 is designed with an arc-shaped structure and the opening faces downward. The bottom end of the circulating return water pipe 46 is located inside the return water guide hood 461. Drainage pipe 473 is fixedly installed at the output end of silent circulation pump 47. Drainage pipe 473 is used to drain the water inside the device after a breeding cycle of forest frogs is completed.

[0031] When using the live breeding preservation device for the Northeast Forest Frog in this embodiment, a suitable site (slightly low-lying area) is selected before the forest frog enters hibernation period, and a pit slightly larger than the semi-buried breeding box 1 is dug. Then, the semi-buried breeding box 1 is transferred into the pit, ensuring that the uppermost third of the semi-buried breeding box 1 is above the ground (i.e., the upper surface of the inlet 333 is flush with the ground). Then, the gap between the semi-buried breeding box 1 and the pit is backfilled with insulating sand. After the semi-buried breeding box 1 is installed, water is injected into the semi-buried breeding box 1. At this point, all the preparations for the overwintering preservation of the forest frog are completed. When the forest frog is about to enter hibernation, the breeding platform 3 is driven upward by the winch 303, so that the breeding platform 3 moves to the middle position. At this time, the upper part of the forest frog hibernation tube 4 above the breeding platform 3 is above the water surface. At this time, a shallow water area is formed inside the semi-buried breeding box 1. The forest frogs enter the interior of the semi-buried breeding box 1 through the external entrance 333 and autonomously select the corresponding forest frog hibernation chamber 41. After the forest frog enters the semi-buried breeding box 1, the winch 303 is controlled to drive the breeding platform 3 downward until the breeding platform 3 moves to the lowest point. At this time, a deep water area is formed inside the semi-buried breeding box 1, the forest frog hibernation tube 4 is completely immersed in the water, and a gap is formed between the top of the forest frog hibernation tube 4 and the water surface. This gap lays the foundation for the subsequent freezing of the water surface. At this time, the roof 33 fits with the inlet 333 to seal the inlet 333, so as to prevent the forest frog's natural enemies from entering the semi-buried breeding box 1. When the outside temperature drops below freezing, the water surface inside the semi-submerged breeding box 1 freezes, forming an ice surface. The water below the ice surface is kept at 0-4℃ due to the temperature of the underground soil. At this time, the frog hibernation tube 4 is located in water at 0-4℃, providing a suitable temperature environment for the frogs' hibernation. During this process, the frogs hibernate in the frog hibernation chamber 41 to preserve their species. During this process, the silent circulation pump 47 operates at low power. The silent circulation pump 47 draws water from the middle of the semi-submerged breeding box 1 through the circulation pumping hose 45 and the main flow pipe 43, and transports this water to below the ice surface through the circulation return pipe 46 to achieve water circulation below the ice surface. The main flow pipe 43... When water is extracted, the internal flow rate increases. At this time, the main flow pipe 43 extracts static water from the frog hibernation chamber 41 through the staggered guide channels 44 and water passage holes 42, so that the water in the frog hibernation chamber 41 can be circulated. In addition, during the circulation process, the temperature of the frog hibernation habitat area composed of the frog hibernation pipes 4 is balanced, thereby ensuring the stability of the frog hibernation environment. Furthermore, when the water passes through the main pumping pipe 472 during the circulation process, the venturi tube composed of the oxygen supply pipe 474 and the main pumping pipe 472 can actively replenish the water with external oxygen, so as to ensure that the frog hibernation chamber 41 always maintains fresh oxygen-rich water, thereby improving the survival rate of frogs overwintering. When spring arrives, the forest frogs end their hibernation and enter their breeding cycle. At this time, the breeding platform 3 is driven upward to its highest point by the winch 303. At this point, a very shallow water area is formed between the upper surface of the breeding platform 3 and the water surface. The hatching slope 32 at one end of the breeding platform 3 is above the water surface. The pebble layer on the upper surface of the breeding platform 3 and the hatching slope 32 create a mudflat ecological environment for the forest frogs to breed. The forest frogs complete mating, egg laying and hatching above the breeding platform 3. After hatching, the tadpole-like forest frogs grow to the metamorphosis zone in the shallow water area above the breeding platform 3, and then use the hatching slope 32 to complete metamorphosis. After metamorphosis, the forest frogs leave the semi-buried breeding box 1 through the inlet 333. Thus, the breeding, overwintering and preservation work of the forest frogs is completed in one cycle.

[0032] It should be noted that the winch 303 and the silent circulating pump 47 involved in this embodiment are both corresponding products in the prior art. They can be controlled by the matching controller or manually. Furthermore, when the winch 333 drives the breeding platform 3 to rise and fall, the breeding platform 3 moves at an extremely low speed to avoid disturbing the forest frogs. It should be further noted that the semi-buried breeding box 1 in this embodiment is also equipped with oxygen sensors, temperature sensors and other sensors for detecting the water body and the state of the frogs. All of the above sensors are corresponding products in the prior art.

[0033] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A germplasm preservation device for the breeding of the Northeast Forest Frog, characterized in that, include: A semi-embedded breeding box (1) has an insulated inner cavity inside; The partition (2) is fixedly installed at the top of the interior of the semi-embedded breeding box (1); The breeding platform (3) is installed inside the semi-embedded breeding box (1) and located on one side of the partition (2). The breeding platform (3) is designed with a hollow structure and has a pebble pad layer (31) on its upper surface. The breeding platform (3) is fixedly installed with an incubation slope (32) at one end near the partition (2). The frog hibernation tube (4) is provided in multiple ways and is installed vertically above the breeding platform (3). Multiple frog hibernation chambers (41) are fixedly installed on the outer wall of the frog hibernation tube (4). The inner wall of the frog hibernation chamber (41) is provided with water passage holes (42) and the water passage holes (42) extend into the interior of the frog hibernation tube (4). A circulating water pumping hose (45) and a circulating water return pipe (46) are provided. The circulating water pumping hose (45) is located below the breeding platform (3) and connected to the bottom end of the frog hibernation pipe (4). The circulating water return pipe (46) is located on the side of the partition (2) away from the breeding platform (3) and its bottom end penetrates the partition (2). A silent circulating pump (47) is provided between the top end of the circulating water return pipe (46) and the top end of the circulating water pumping hose (45).

2. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: The main flow pipe (43) is fixedly installed inside the frog hibernation tube (4). The upper flow guide hood (431) and the lower flow guide hood (432) are fixedly installed at both ends of the main flow pipe (43). The upper flow guide hood (431) and the lower flow guide hood (432) are located at both ends inside the frog hibernation tube (4). The upper flow guide hood (431), the lower flow guide hood (432), the main flow pipe (43) and the frog hibernation tube (4) form a flow guide cavity (433) for guiding water through the frog hibernation chamber (41). The guide channel (44) is provided in multiple ways and is located on the outside of the main flow pipe (43), and the multiple guide channels (44) are respectively interleaved with multiple frog hibernation chambers (41).

3. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: The main return water pipe (471) and the main pumping water pipe (472) are fixedly installed at the output end of the silent circulation pump (47) and connected to the top end of the circulation return water pipe (46). The main pumping water pipe (472) is fixedly installed at the input end of the silent circulation pump (47) and connected to the top end of the circulation pumping hose (45). An oxygen supply pipe (474) is fixedly installed in the middle of the main return water pipe (471) and its inner diameter is smaller than that of the main return water pipe (471). A valve is installed between the oxygen supply pipe (474) and the main return water pipe (471).

4. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: The return water guide hood (461) is fixedly installed on the middle part of the side of the partition (2) near the breeding platform (3). The return water guide hood (461) is designed with an arc-shaped structure and the opening faces downward. The bottom end of the circulating return water pipe (46) is located inside the return water guide hood (461).

5. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: Guide sleeves (301) and guide rods (302), the guide sleeves (301) are provided in multiple and are all fixedly installed on the upper surface of the breeding platform (3), the guide rods (302) are provided in multiple and are all fixedly installed inside the semi-embedded breeding box (1), and the multiple guide rods (302) are slidably installed inside the multiple guide sleeves (301); A winch (303) is fixedly installed at the top of the semi-buried breeding box (1) and its input end is fixedly connected to the upper surface of the breeding platform (3). The winch (303) is used to provide power for adjusting the height of the breeding platform (3). The breeding platform (3) is adjusted to the corresponding height during the hibernation period, hibernation period and emergence period of the forest frog.

6. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: A canopy (33) is fixedly installed above the breeding platform (3), and multiple support rods (34) are installed between the lower surface of the canopy (33) and the upper surface of the breeding platform (3). The entrance (333) is fixedly installed at the top of the semi-buried breeding box (1) and located between the roof (33) and the breeding platform (3). The entrance (333) is used for the frogs to enter and exit.

7. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 6, characterized in that, Also includes: Ventilation hood (331), which is fixedly installed on the upper surface of the ceiling (33); The ventilation net (332) is a frame structure and is fixedly installed between the ventilation hood (331) and the roof (33). The ventilation net (332) is used to realize the ventilation of the semi-buried breeding box (1) after the roof (33) and the inlet (333) are attached.

8. The germplasm preservation device for the breeding of Northeast Forest Frog according to claim 1, characterized in that, Also includes: An external inspection door (11) is located at the top of the outer wall of the semi-submerged breeding box (1). When the external inspection door (11) is opened, the water condition between the partition (2) and the semi-submerged breeding box (1) can be checked. An inner inspection door (21) is located at the top of the partition (2). When the inner inspection door (21) is opened, the state of the ice surface above the breeding platform (3) can be checked.

9. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: The frog hibernation chamber (41) is designed with a cup-shaped structure.

10. The germplasm preservation and live breeding device for the Northeast Forest Frog as described in claim 1, characterized in that, Also includes: The drain pipe (473) is fixedly installed at the output end of the silent circulating pump (47).

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