A digital PCR droplet generation device

Abstract

The application discloses a digital PCR micro-droplet generation device, and relates to the field of microbiology devices, which comprises a digital PCR box body, a moving part and a micro-droplet generation part, wherein the digital PCR box body is provided with a box door, the digital PCR box body is provided with an installation groove, the installation groove is slidably connected with a pull-out plate, and a plurality of PCR reagent grooves are arranged at one end of the pull-out plate; when the output end of the third motor drives the rotating disc to rotate, the slide bar is located at the eccentric position of the rotating disc, the sliding ring is located in the through hole, the slide bar is located inside the sliding ring, the two sides of the sliding ring are fixedly connected with the plug blocks through the connecting rods, the slide bar pushes the sliding ring and the lower installation table to reciprocate relative to the upper installation table, high-throughput and fully-automatic micro-droplet generation is realized, and the PCR reagent in the PCR dropper can be dispersed into the droplet groove efficiently and quickly.

Description

Technical Field

[0001] This invention relates to the field of microbiological devices, and more specifically, to a digital PCR droplet generation device. Background Technology

[0002] The digital PCR droplet generator is a key component of digital PCR technology. It disperses the PCR reaction system into thousands of independent droplets, thereby achieving parallel amplification at the single-molecule level. This technology features high sensitivity, high specificity, high tolerance, and absolute quantification, and has wide applications in life science research and clinical fields.

[0003] However, in existing technologies, droplet generation is usually controlled by pressure-driven devices and flow regulation devices to generate PCR reagents on a chip. Due to blockage by impurities at the shearing opening or rear end of the chip channel, the generated droplets are uneven in size and are greatly affected by contamination. Furthermore, the droplet generation process requires the droplets to be slowly spread throughout the chip channel on one side, which is particularly inefficient for detecting low-abundance nucleic acids. To address this, we designed a digital PCR droplet generation device. Summary of the Invention

[0004] In view of the problems in existing technologies, such as the significant impact of contamination on droplet generation and the low detection efficiency, especially for low-abundance nucleic acids, the purpose of this invention is to provide a digital PCR droplet generation device.

[0005] To solve the above problems, the present invention adopts the following technical solution.

[0006] A digital PCR droplet generation device includes a digital PCR chamber, a moving part, and a droplet generation part. The digital PCR chamber is provided with a door, and the digital PCR chamber is provided with an installation groove. A pull plate is slidably connected in the installation groove. One end of the pull plate is provided with a plurality of PCR reagent slots, and the other end is provided with a plurality of PCR dropper slots. A drop plate is provided on one side of the pull plate, and the drop plate is provided with a plurality of drop slots.

[0007] The moving part is mounted on the digital PCR chamber, and the moving part is used to drive the droplet generation part to move within the three-dimensional space of the digital PCR chamber.

[0008] The droplet generating unit is mounted on the moving part, and the droplet generating unit includes a vibration component for vibrating and dripping liquid onto the dripping tank.

[0009] Furthermore, a set of limiting grooves is provided on the inner wall of the digital PCR chamber, and a first screw is installed in the limiting grooves.

[0010] Furthermore, the moving part includes:

[0011] A first motor is fixedly installed on the outer wall of the digital PCR box. The output end of the first motor is coaxially fixedly connected to one of the first screws. Each of the first screws is coaxially fixedly connected to a pulley. The same transmission belt is wound around the pulleys. A slider is provided in the limiting groove. The slider is provided with a threaded hole that matches the thread of the first screw. A moving stage is provided below the slider.

[0012] A second motor is fixedly installed on the moving platform, and a second slider is provided on the moving platform. A lead screw mechanism is provided between the second motor and the second slider. A lifting hydraulic cylinder is fixedly installed on the second slider, and the end of the lifting hydraulic cylinder away from the second slider is fixedly connected to the microdroplet generating part.

[0013] Furthermore, the droplet generation unit also includes:

[0014] An upper mounting platform is connected to the output end of the lifting hydraulic cylinder, and a lower mounting platform is provided below the upper mounting platform. Multiple mounting pipes are provided at the end of the lower mounting platform away from the upper mounting platform, and the vibration component is arranged between the upper mounting platform and the lower mounting platform.

[0015] The vibration assembly includes: a third motor disposed within the upper mounting platform; a through hole is provided at one end of the upper mounting platform near the lower mounting platform; a turntable is coaxially fixedly connected to the output end of the third motor; the turntable is coaxially disposed within the through hole; a sliding rod is provided at the end of the turntable away from the third motor; a set of slots is provided on the lower mounting platform; a sliding ring is provided on the outer side of the sliding rod; connecting rods are fixedly connected to both sides of the sliding ring; a plug is fixedly connected to the end of the connecting rod away from the sliding ring; and the plug is fixedly engaged within the slot.

[0016] Furthermore, the turntable is provided with an adjustment component, and the slide rod is disposed on the adjustment component. The slide rod is eccentrically disposed on the turntable through the adjustment component.

[0017] Furthermore, the adjustment component includes:

[0018] A fixed ring is fixedly installed on the turntable. A sliding groove is provided on the inner side wall of the fixed ring. A second screw is provided in the sliding groove. A fourth motor is fixedly installed on the upper mounting platform. The output end of the fourth motor is coaxially and fixedly connected to the second screw.

[0019] A slider three is fixedly connected to the side wall of the slide rod. The slider three is located in the slide groove and has a threaded hole that matches the thread of the second screw.

[0020] Furthermore, the PCR reagent slot contains PCR reagents, and the PCR dropper slot contains PCR droppers; a limiting slot is provided on the inner wall of the mounting slot, and multiple rollers are provided on the side wall of the pull plate, with the rollers set in the limiting slot; a sealing groove is provided on the side wall of the digital PCR box, and a sealing plate is provided at the end of the pull plate near the sealing groove.

[0021] Furthermore, the upper mounting platform is equipped with a pneumatic pressure control device and a constant flow control device, and a connecting air pipe is connected between the pneumatic pressure control device and the constant flow control device. The end of the connecting air pipe away from the upper mounting platform is connected to the mounting pipe.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] (1) This application uses the vibration component in the droplet generation section to make the slide rod push the sliding ring and the lower mounting stage reciprocate relative to the upper mounting stage, thereby realizing high-throughput and fully automatic droplet generation, which facilitates efficient and rapid dispersion of PCR reagents in the PCR dropper into the droplet tank; similar to 3D printing, the PCR reagents in the PCR dropper are dispersed into the droplet tank, which greatly improves the droplet generation rate and saves high chip costs compared with the prior art.

[0024] (2) This application uses a control panel to control the adjustment component to adjust the eccentric position of the slide bar on the turntable according to the required microdroplet particle size and dispersion degree. When the slide bar rotates with the turntable, the maximum distance of one unit of reciprocating movement of the slide bar in the sliding ring is also different, thereby realizing the adjustment of the particle size and dispersion degree of microdroplet generation.

[0025] (3) This application places the prepared PCR reagents and the required PCR droppers in the PCR reagent slot and PCR dropper slot on the pull-out plate, respectively, and installs the dropper plate. This facilitates the replacement of expensive chips with 3D printed flat-lay chips, avoiding the blockage caused by impurities at the cut opening or back end of the chip flow channel, which leads to uneven droplet size. At the same time, it is less affected by contamination. It facilitates subsequent PCR amplification and detection, and the operation is simple and convenient. It has a significant improvement in efficiency in detecting low abundance nucleic acids. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall structure of a digital PCR droplet generation device provided in an embodiment of this application;

[0027] Figure 2 This is a schematic diagram of the installation structure of the moving part and the droplet generation part of a digital PCR droplet generation device provided in an embodiment of this application;

[0028] Figure 3A schematic diagram of the internal structure of the droplet generation section of a digital PCR droplet generation device provided in this application embodiment. Figure 1 ;

[0029] Figure 4 A schematic diagram of the vibration component structure of a digital PCR droplet generation device provided in this application embodiment;

[0030] Figure 5 A schematic diagram of the internal structure of the droplet generation section of a digital PCR droplet generation device provided in this application embodiment. Figure 2 ;

[0031] Figure 6 This is a schematic diagram of the adjustment component structure of a digital PCR droplet generation device provided in an embodiment of this application;

[0032] Figure 7 This is a schematic diagram of the ring frame structure of a digital PCR droplet generation device provided in an embodiment of this application.

[0033] Explanation of the labels in the diagram:

[0034] 1. Digital PCR chamber; 11. Chamber door; 12. Moving part; 121. Moving stage; 122. Slider one; 13. First motor; 14. First screw; 141. Transmission belt; 15. Second motor; 151. Slider two; 16. Lifting hydraulic cylinder;

[0035] 2. Droplet generation section; 21. Upper mounting platform; 211. Through hole; 22. Lower mounting platform; 23. Connecting gas pipe; 24. Mounting pipe;

[0036] 3. Mounting slot; 31. Pull-out plate; 311. PCR reagent holder; 312. PCR dropper holder; 32. Dropper plate;

[0037] 4. Vibration assembly; 41. Third motor; 42. Turntable; 43. Fixed ring; 431. Slide groove; 432. Fourth motor; 433. Second screw; 44. Slide rod; 441. Sliding block; 45. Sliding ring; 46. Connecting rod; 47. Insert block; 471. Slot. Detailed Implementation

[0038] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0039] Example 1, please refer to Figures 1 to 7This is the first embodiment of the present invention. This embodiment provides a digital PCR droplet generation device, which includes a digital PCR box 1, a moving part 12 and a droplet generation part 2. The digital PCR box 1 is provided with a box door 11. The digital PCR box 1 is provided with an installation groove 3. A pull plate 31 is slidably connected in the installation groove 3. A plurality of PCR reagent slots 311 are provided at one end of the pull plate 31 and a plurality of PCR dropper slots 312 are provided at the other end. A drop plate 32 is provided on one side of the pull plate 31 and a plurality of drop slots are provided on the drop plate 32.

[0040] PCR reagents are placed in the PCR reagent tank 311, and PCR droppers are placed in the PCR dropper tank 312. A limiting slot is provided on the inner wall of the mounting tank 3, and multiple rollers are provided on the side wall of the pull plate 31. The rollers are set in the limiting slot. A sealing groove is provided on the side wall of the digital PCR box 1, and a sealing plate is provided at the end of the pull plate 31 near the sealing groove.

[0041] The upper mounting platform 21 is equipped with a pneumatic control device and a constant flow control device. A connecting air pipe 23 connects the pneumatic control device and the constant flow control device. The end of the connecting air pipe 23 away from the upper mounting platform 21 is connected to the mounting pipe 24.

[0042] During use: First, open the door 11, pull out the pull plate 31, place the prepared PCR reagent and the required PCR dropper into the PCR reagent slot 311 and PCR dropper slot 312 on the pull plate 31 respectively, and install the dropper plate 32. The moving part 12 drives the microdropper generation part 2 to install the PCR dropper in the PCR dropper slot 312 with the microdropper generation part 2.

[0043] The PCR reagent in the PCR reagent tank 311 is drawn in by the air pressure control device and the constant flow control device, and then the PCR reagent drawn in by the moving part 12 and the microdroplet generating part 2 is dispersed into the dropper tank on the dropper plate 32 by the cooperation of the moving part 12 and the microdroplet generating part 2.

[0044] Here, the droplet generation unit 2 causes the PCR reagent drawn into the PCR dropper to reciprocate during normal droplet dispensing. As the droplet reciprocates, the droplet nozzle is no longer in a single position. By adjusting the air pressure control device and the constant flow control device, compared to the existing technology where the droplet generation process requires slowly filling the entire chip channel through one side of the chip, this device increases the droplet drip rate in the PCR droplet, improves the droplet generation efficiency, facilitates subsequent PCR amplification and detection, and is simple and convenient to operate, especially showing a significant improvement in efficiency when detecting low-abundance nucleic acids.

[0045] Example 2, as Figure 1 and Figure 2As shown, this is the second embodiment of the present invention. Unlike the previous embodiment, this embodiment provides a moving part 12 for a digital PCR droplet generation device. The moving part 12 is disposed on the digital PCR housing 1 and is used to drive the droplet generation part 2 to move within the three-dimensional space of the digital PCR housing 1. The moving part 12 includes:

[0046] A first motor 13 is fixedly installed on the outer wall of the digital PCR chamber 1. The output end of the first motor 13 is coaxially fixedly connected to one of the first screws 14. Each of the first screws 14 is coaxially fixedly connected to a pulley. The same transmission belt 141 is wound around the pulleys. A set of limiting grooves is opened on the inner wall of the digital PCR chamber 1. The first screws 14 are installed in the limiting grooves. A slider 122 is provided in the limiting grooves. The slider 122 is provided with a threaded hole that matches the thread of the first screw 14. A moving stage 121 is provided below the slider 122.

[0047] Furthermore, a second motor 15 is fixedly installed on the moving stage 121, and a second slider 151 is provided on the moving stage 121. A screw mechanism is provided between the second motor 15 and the second slider 151. A lifting hydraulic cylinder 16 is fixedly installed on the second slider 151, and the end of the lifting hydraulic cylinder 16 away from the second slider 151 is fixedly connected to the microdroplet generating part 2.

[0048] During use: The output end of the first motor 13 drives one of the first screws 14 to rotate. Since a group of first screws 14 are connected by a transmission belt 141 and a set of pulleys, and multiple sliders 122 on the moving stage 121 are respectively set in the limiting grooves on the inner wall of the digital PCR box 1, and the sliders 122 are provided with threaded holes that are compatible with the threads of the first screws 14, when a group of first screws 14 rotate, the moving stage 121 moves horizontally along the length of the digital PCR box 1 inside the digital PCR box 1.

[0049] Similarly, the output of the second motor 15 drives the slider 151 to move the lifting hydraulic cylinder 16 horizontally along the width of the digital PCR chamber 1. The lifting hydraulic cylinder 16 drives the droplet generation unit 2 to move vertically along the height of the digital PCR chamber 1, which facilitates rapid droplet generation similar to 3D printing.

[0050] Example 3, as Figures 3 to 7 As shown, this is the third embodiment of the present invention. Unlike the previous embodiment, this embodiment provides a droplet generation unit 2 of a digital PCR droplet generation device. The droplet generation unit 2 is mounted on the moving part 12. The droplet generation unit 2 includes a vibration component 4 for vibrating and dropping liquid on the droplet tank.

[0051] The droplet generation unit 2 also includes an upper mounting platform 21 connected to the output end of the lifting hydraulic cylinder 16. A steering mechanism is provided between the output end of the lifting hydraulic cylinder 16 and the upper mounting platform 21 to ensure that the droplet generation unit 2 can be adjusted to rotate around the output end of the lifting hydraulic cylinder 16. This, in conjunction with the moving part 12, enables rapid droplet generation similar to 3D printing. A lower mounting platform 22 is provided below the upper mounting platform 21. Multiple mounting tubes 24 are provided at the end of the lower mounting platform 22 away from the upper mounting platform 21. The vibration component 4 is located between the upper mounting platform 21 and the lower mounting platform 22.

[0052] The vibration assembly 4 includes: a third motor 41 disposed in the upper mounting platform 21; a through hole 211 is provided at one end of the upper mounting platform 21 near the lower mounting platform 22; a turntable 42 is coaxially fixedly connected to the output end of the third motor 41; the turntable 42 is coaxially disposed in the through hole 211; a slide rod 44 is provided at one end of the turntable 42 away from the third motor 41; a set of slots 471 is provided on the lower mounting platform 22; a sliding ring 45 is provided on the outer side of the slide rod 44; a connecting rod 46 is fixedly connected to both sides of the sliding ring 45; a plug 47 is fixedly connected to one end of the connecting rod 46 away from the sliding ring 45; and the plug 47 is fixedly locked in the slot 471.

[0053] During use: When it is necessary to drip liquid into the dripping tank on the dripping plate 32, the displacement of the micro-droplet generating part 2 is controlled by the moving part 12. Since the sliding rod 44 is located at the eccentric position of the turntable 42, the sliding ring 45 is located in the through hole 211. At the same time, the sliding rod 44 is located inside the sliding ring 45, and both sides of the sliding ring 45 are fixedly connected to the plug 47 by the connecting rod 46. Since the lower mounting platform 22 has a slot 471 on the side close to the upper mounting platform 21 to facilitate the fixing and locking of the plug 47;

[0054] Therefore, when the turntable 42 is driven to rotate by the output end of the third motor 41, the eccentrically set slide rod 44 rotates around the center of the turntable 42, and the slide rod 44 is located inside the sliding ring 45. The sliding ring 45 is slidably connected to the upper mounting platform 21 through the connecting shaft. Since the sliding ring 45 is fixedly connected to the lower mounting platform 22 through the insert block 47, when the slide rod 44 rotates around the center of the turntable 42, it pushes the sliding ring 45 and the lower mounting platform 22 to reciprocate relative to the upper mounting platform 21.

[0055] During the reciprocating oscillation of the droplets, since the PCR dropper nozzle is no longer in a single position, the device increases the droplet rate within the PCR dropper by adjusting the air pressure control device and the constant flow control device, achieving high-throughput, fully automated droplet generation. This facilitates the efficient and rapid dispersion of PCR reagents from the PCR dropper into the dropping chamber. Similar to 3D printing, the dispersion of PCR reagents from the PCR dropper into the dropping chamber greatly improves droplet generation and dispersion efficiency, saving on the high cost of chips compared to existing technologies.

[0056] Please see Figure 6 The turntable 42 is equipped with an adjustment assembly, and a slide rod 44 is mounted on the adjustment assembly. The slide rod 44 is eccentrically mounted on the turntable 42 via the adjustment assembly. The adjustment assembly includes:

[0057] A fixed ring 43 is fixedly installed on the turntable 42. A sliding groove 431 is provided on the inner side wall of the fixed ring 43. A second screw 433 is provided in the sliding groove 431. A fourth motor 432 is fixedly installed on the upper mounting platform 21. The output end of the fourth motor 432 is coaxially and fixedly connected to the second screw 433.

[0058] Among them, a slider three 441 is fixedly connected to the side wall of the slide rod 44. The slider three 441 is located in the slide groove 431. The slider three 441 is provided with a threaded hole that matches the thread of the second screw 433.

[0059] The effect of this setting is that since the slider 441 fixedly connected to the slider 44 is set in the groove 431 on the inner side wall of the fixed ring 43, and the slider 441 is provided with a threaded hole that matches the thread of the second screw 433, when the output end of the fourth motor 432 is controlled by the control board to drive the second screw 433 to rotate, the slider 44 moves within the fixed ring 43, which makes it easy to adjust the eccentric position of the slider 44 on the turntable 42.

[0060] Because the slide rod 44 is eccentrically positioned on the turntable 42, the maximum distance of one unit of reciprocating movement of the slide rod 44 within the sliding ring 45 is also different as the slide rod 44 rotates with the turntable 42.

[0061] As the slide bar 44 gradually approaches the center of the turntable 42, the maximum distance of one unit of reciprocating movement of the slide bar 44 within the sliding ring 45 gradually decreases, that is, the vibration frequency of the lower mounting platform 22 gradually decreases; conversely, the vibration frequency of the lower mounting platform 22 gradually increases. When the slide bar 44 is located at the center of the turntable 42, the lower mounting platform 22 and the upper mounting platform 21 are relatively stationary.

[0062] While keeping the air pressure control device and the constant flow control device stationary, by adjusting the slide bar 44 to gradually move away from the center of the turntable 42, the vibration frequency of the lower mounting platform 22 gradually increases. Under the action of inertia, the particle size and dispersion of the microdroplets at the PCR dropper nozzle also increase, thereby achieving the adjustment of the particle size and dispersion of the generated microdroplets.

[0063] As can be seen from the above, the working principle of this application is as follows:

[0064] First, open the door 11 and pull out the pull plate 31. Place the prepared PCR reagents and the required PCR droppers into the PCR reagent slot 311 and PCR dropper slot 312 on the pull plate 31, respectively. Then, install the dropper plate 32. The moving part 12 drives the microdroplet generating part 2 to install the PCR dropper in the PCR dropper slot 312. The PCR reagents in the PCR reagent slot 311 are drawn in by the air pressure control device and the constant flow control device. Then, the PCR reagents drawn in by the moving part 12 and the vibration component 4 are dispersed into the dropper slot on the dropper plate 32 through the cooperation of the moving part 12 and the vibration component 4.

[0065] The output of the first motor 13 drives one of the first screws 14 to rotate. Since the set of first screws 14 are connected by a transmission belt 141 and a set of pulleys, and multiple sliders 122 on the moving stage 121 are respectively set in the limiting grooves on the inner wall of the digital PCR box 1, and the sliders 122 are provided with threaded holes that are compatible with the threads of the first screws 14, the moving stage 121 moves horizontally along the length of the digital PCR box 1 when the set of first screws 14 rotates. Similarly, the output of the second motor 15 drives the second slider 151 to drive the lifting hydraulic cylinder 16 to move horizontally along the width of the digital PCR box 1, and the lifting hydraulic cylinder 16 drives the droplet generation part 2 to move vertically along the height of the digital PCR box 1.

[0066] When it is necessary to drip liquid into the dripping groove on the dripping plate 32, the displacement of the micro-droplet generating part 2 is controlled by the moving part 12. Since the sliding rod 44 is located at the eccentric position of the turntable 42, the sliding ring 45 is located in the through hole 211, and the sliding rod 44 is located inside the sliding ring 45. Both sides of the sliding ring 45 are fixedly connected to the plug 47 by the connecting rod 46. Since the lower mounting platform 22 has a slot 471 on the side close to the upper mounting platform 21 to facilitate the fixing and locking of the plug 47, when the turntable 42 is driven to rotate by the output end of the third motor 41, the sliding rod 44 pushes the sliding ring 45 and the lower mounting platform 22 to reciprocate relative to the upper mounting platform 21.

[0067] Furthermore, since the slider 441 fixedly connected to the slide rod 44 is located in the groove 431 on the inner side wall of the fixed ring 43, and the slider 441 has a threaded hole that matches the thread of the second screw 433, when the output end of the fourth motor 432 is controlled by the control board to drive the second screw 433 to rotate, the slide rod 44 moves within the fixed ring 43, which facilitates the adjustment of the eccentric position of the slide rod 44 on the turntable 42. As the slide rod 44 gradually approaches the center of the turntable 42, the maximum distance of one unit of reciprocating movement of the slide rod 44 within the sliding ring 45 gradually decreases, that is, the vibration frequency of the lower mounting platform 22 gradually decreases; conversely, the vibration frequency of the lower mounting platform 22 gradually increases. When the slide rod 44 is located at the center of the turntable 42, the lower mounting platform 22 and the upper mounting platform 21 are relatively stationary.

[0068] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and its improved concepts, should be covered within the scope of protection of the present invention.

Claims

1. A digital PCR droplet generation device, characterized in that, include: The digital PCR chamber (1), the moving part (12), and the droplet generating part (2) are provided. The digital PCR chamber (1) is provided with a door (11). The digital PCR chamber (1) is provided with an installation slot (3). A pull plate (31) is slidably connected in the installation slot (3). A plurality of PCR reagent slots (311) are provided at one end of the pull plate (31), and a plurality of PCR dropper slots (312) are provided at the other end. A drop plate (32) is provided on one side of the pull plate (31), and a plurality of drop slots are provided on the drop plate (32). The moving part (12) is disposed on the digital PCR chamber (1), and the moving part (12) is used to drive the droplet generating part (2) to move in the three-dimensional space of the digital PCR chamber (1); The droplet generating unit (2) is mounted on the moving unit (12), and the droplet generating unit (2) includes a vibration component (4) for vibrating the droplets on the dripping tank. A set of limiting grooves is provided on the inner wall of the digital PCR box (1), and a first screw (14) is installed in the limiting grooves. The moving part (12) includes: A first motor (13) is fixedly installed on the outer wall of the digital PCR box (1). The output end of the first motor (13) is coaxially fixedly connected to one of the first screws (14). Each of the first screws (14) is coaxially fixedly connected to a pulley. The same transmission belt (141) is wound around the pulleys. A slider (122) is provided in the limiting groove. A threaded hole that matches the thread of the first screw (14) is opened on the slider (122). A moving stage (121) is provided below the slider (122). A second motor (15) is fixedly installed on the moving stage (121), and a second slider (151) is provided on the moving stage (121). A screw mechanism is provided between the second motor (15) and the second slider (151). A lifting hydraulic cylinder (16) is fixedly installed on the second slider (151), and the end of the lifting hydraulic cylinder (16) away from the second slider (151) is fixedly connected to the microdroplet generating part (2). The droplet generation unit (2) further includes: An upper mounting platform (21) is connected to the output end of the lifting hydraulic cylinder (16). A lower mounting platform (22) is provided below the upper mounting platform (21). A plurality of mounting pipes (24) are provided at the end of the lower mounting platform (22) away from the upper mounting platform (21). The vibration component (4) is located between the upper mounting platform (21) and the lower mounting platform (22). The vibration assembly (4) includes: a third motor (41) disposed in the upper mounting platform (21), a through hole (211) is provided at one end of the upper mounting platform (21) near the lower mounting platform (22), a turntable (42) is coaxially fixedly connected to the output end of the third motor (41), the turntable (42) is coaxially disposed in the through hole (211), a slide rod (44) is provided at one end of the turntable (42) away from the third motor (41), a set of slots (471) is provided on the lower mounting platform (22), a sliding ring (45) is provided on the outside of the slide rod (44), a connecting rod (46) is fixedly connected to both sides of the sliding ring (45), a plug (47) is fixedly connected to one end of the connecting rod (46) away from the sliding ring (45), and the plug (47) is fixedly locked in the slot (471).

2. The digital PCR droplet generation device according to claim 1, characterized in that: The turntable (42) is provided with an adjustment component, and the slide rod (44) is set on the adjustment component. The slide rod (44) is eccentrically set on the turntable (42) through the adjustment component.

3. The digital PCR droplet generation device according to claim 2, characterized in that: The adjustment component includes: A fixing ring (43) is fixedly installed on the turntable (42). A groove (431) is provided on the inner side wall of the fixing ring (43). A second screw (433) is provided in the groove (431). A fourth motor (432) is fixedly installed on the upper mounting platform (21). The output end of the fourth motor (432) is coaxially and fixedly connected to the second screw (433). A slider three (441) is fixedly connected to the side wall of the slide rod (44). The slider three (441) is located in the slide groove (431). The slider three (441) is provided with a threaded hole that matches the thread of the second screw (433).

4. The digital PCR droplet generation device according to claim 1, characterized in that: The PCR reagent slot (311) contains PCR reagents, and the PCR dropper slot (312) contains PCR droppers; the inner wall of the mounting slot (3) has a limiting slot, the side wall of the pull plate (31) has multiple rollers, the rollers are set in the limiting slot, the side wall of the digital PCR box (1) has a sealing groove, and the end of the pull plate (31) near the sealing groove has a sealing plate.

5. The digital PCR droplet generation device according to claim 1, characterized in that: The upper mounting platform (21) is equipped with a pneumatic control device and a constant flow control device. A connecting air pipe (23) connects the pneumatic control device and the constant flow control device. The end of the connecting air pipe (23) away from the upper mounting platform (21) is connected to the mounting pipe (24).

Images