Culture device for filamentous fungus hyphae and control system thereof

Abstract

The invention relates to the technical field of fungus culture, in particular to a filamentous fungus hypha culture device and a control system thereof.The filamentous fungus hypha culture device comprises a vertical frame, two sets of transverse frames are installed on the side wall of the vertical frame, and the two sets of transverse frames are symmetrically installed front and back; a separation structure is mounted on the vertical frame, a cutting structure is mounted on the transverse frame, and a fungus frame is placed on the separation structure; a movable groove is formed in the inner wall of the transverse frame, and a collecting frame is fixedly installed on the inner wall of the vertical frame. The separating structure comprises a fixing frame, the fixing frame is fixedly installed on the inner wall of the transverse frame, and guide cylinders are fixedly installed at the front end and the rear end of the inner side of the fixing frame. According to the culture device, the blades capable of horizontally moving are arranged on the inner sides of the transverse frames, hyphae wound on the outer sides of the movable frames can be cut off, adhesion between the fungus frames and the movable frames can be relieved, the fungus frames can be conveniently taken out, operation is convenient, labor is saved, functionality is high, and application and popularization are facilitated.

Description

Technical Field

[0001] This invention relates to the field of fungal culture technology, specifically to a device and control system for culturing filamentous fungal hyphae. Background Technology

[0002] When culturing fungi, the fungi are often placed on a culture rack for cultivation. After the growth period is over, the fungi are taken out. Most existing culture racks are arranged in a matrix to allow as many fungi as possible to be cultivated in the same amount of time, thereby increasing the productivity of fungi.

[0003] In response, Chinese patent application number CN202211339636.X discloses a functionally adjustable device for edible fungi cultivation, comprising a base, a support, a top plate, an adjustment mechanism, a cultivation rack mechanism, and a cutting mechanism. The support is fixedly installed on the upper end of the base, the top plate is fixedly installed on the upper end of the support, the adjustment mechanism is located at the outer end of the support, the cultivation rack mechanism is located at the outer end of the adjustment mechanism, and the cutting mechanism is located at the upper end of the base. The adjustment mechanism includes a first connecting rod, a first connecting frame, a first rotating shaft, a second connecting rod, and a second connecting frame. When the cultivation racks on this device need to be stored, the three rotating shafts are rotated sequentially from top to bottom, with the lengths of the three rotating shafts increasing sequentially downwards. This can drive the cultivation racks to rotate, causing the cultivation racks to flip upwards and multiple cultivation racks to be stacked vertically together, thereby saving more space.

[0004] This device uses a rotatable culture rack with a vertically movable cutting blade to cut off the roots of the fungal spawn. However, the cutting blade is vertically oriented, so the culture rack must be flipped downwards to fit the blade when cutting the roots. After flipping, the culture rack is vertical, making it difficult to place the spawn box stably on it. Therefore, workers need to hold the spawn box by hand; otherwise, the spawn box will shift relative to the culture rack, pulling on the mycelium and causing the spawn inside to be stretched, deformed, and loosened. This is laborious and carries the risk of damaging the spawn, making it difficult to promote and use the device.

[0005] Therefore, in order to solve the above problems, a cultivation device for filamentous fungal hyphae and its control system are proposed. Summary of the Invention

[0006] The purpose of this invention is to provide a cultivation device and control system for filamentous fungal hyphae, to solve the problems mentioned in the prior art. In the prior art, the device uses a rotatable cultivation rack with a vertically movable cutting blade to cut off the roots of the fungus. However, the cutting blade is vertically positioned, so the cultivation rack must be flipped downwards to fit the blade. After flipping, the cultivation rack is vertical, making it difficult to stably place the fungal frame on it. Therefore, workers need to manually support the frame; otherwise, the frame will shift relative to the rack, pulling on the hyphae and causing the fungus inside to stretch, deform, and loosen. This is laborious and carries the risk of damaging the fungus, making the device difficult to widely use.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a cultivation device for filamentous fungal hyphae and its control system, comprising: a vertical frame, wherein two sets of horizontal frames are installed on the side wall of the vertical frame, and the two sets of horizontal frames are symmetrically installed front and back; A separation structure is installed on the vertical frame, a cutting structure is installed on the horizontal frame, and a mushroom basket is placed on the separation structure; The inner wall of the horizontal frame is provided with a movable groove, and a collection frame is fixedly installed on the inner wall of the vertical frame. The separation structure includes a fixed frame, which is fixedly installed on the inner wall of the horizontal frame. Guide cylinders are fixedly installed at the front and rear ends of the inner side of the fixed frame. Rotating shafts are inserted into the left and right sides of the fixed frame. A semi-circular abutment block is fixedly installed in the middle of the rotating shaft. The surface of the semi-circular abutment block has a concave surface. A first worm gear is fixedly installed at the right end of the rotating shaft. An installation frame is fixedly installed at the right end of the inner side of the vertical frame. A rotating rod is movably installed on the inner side of the installation frame through a bearing. A first worm gear is fixedly installed in the middle of the rotating rod. A second worm gear is fixedly installed at the rear end of the rotating rod. A handle is fixedly installed at the front end of the rotating rod. A movable frame is movably installed on the top surface of the fixed frame. Guide columns are fixedly connected to the front and rear ends of the bottom surface of the movable frame. Grooves are opened on the left and right side walls of the movable frame. The cutting structure includes a threaded rod, which is movably mounted on the inner side of a set of crossbeams on the rear side via a bearing. A second worm gear is fixedly connected to the right end of the threaded rod. A guide rod is fixedly mounted on the inner side of a set of crossbeams on the front side. A movable frame is slidably mounted on the guide rod. A blade is provided in the middle of the movable frame.

[0008] Preferably, the collection frame is located on the lower side of the separation structure and is installed at an angle.

[0009] Preferably, the fixing frame is perpendicular to the rotation axis, the semi-circular abutment block is located inside the fixing frame, the semi-circular abutment block corresponds one-to-one with the fixing frame, and the arc length of the semi-circular abutment block is greater than half of the whole.

[0010] Preferably, the thickness of the semicircular abutment block is greater than the thickness of the concave surface, and two adjacent sets of semicircular abutment blocks are symmetrically arranged front and back, with the arc edge of the semicircular abutment block fitting against the bottom surface of the movable frame.

[0011] Preferably, the first worm gear meshes with the first worm, and the second worm meshes with the second worm gear.

[0012] Preferably, the threaded rod passes through the rear end of the movable frame and is threadedly connected to the movable frame, and the movable frame protrudes from inside the crossbeam via a movable slot.

[0013] Preferably, the blade is located inside the right side of the vertical frame, the blade is located on the upper side of the fixed frame, and the blade is perpendicular to the rotation axis.

[0014] A culture control system for filamentous fungal hyphae: S1. Basic Culture Medium Formulation PDA medium (most commonly used): Potato starch: 4-6 g / L • Glucose: 20 g / L • Agar: 15 g / L (solid) Sterilize at 121℃ for 20 minutes. S2. Cultivation Steps Solid culture: 1. Inoculation: Use an inoculation needle to pick up spores or mycelial blocks and spot or streak them onto a plate. 2. Incubation: Incubate upside down at 25-28℃ to avoid condensation. 3. Time: 3-7 days until the mycelium fully covers the plate. Liquid culture: 1. Inoculation: Add spore suspension at an inoculation rate of 5-10%. 2. Conditions: 25-28℃, shake culture at 150-200 rpm. 3. Time: 2-5 days, the mycelium grows in a flocculent manner. S3. Key Parameters • Temperature: 25-30℃ (most bacterial species) pH: 5.5-6.5 • Humidity: >85% (solid culture) • Lighting: Dark or dim light S4. Precautions 1. Strict aseptic technique 2. Regularly observe the morphology and color of the mycelium. 3. Transfer in a timely manner to prevent spore aging. 4. Ensure adequate aeration during liquid culture. S5. Frequently Asked Questions • Contamination: Strengthen sterilization and operational procedures Slow growth: Check temperature, nutrients, and pH. • Sparse hyphae: Optimize carbon-nitrogen ratio and trace elements Storage: Store at 4℃ for short periods, or at -80℃ for long periods in glycerol tubes.

[0015] Compared with the prior art, the beneficial effects of the present invention are: the cultivation device of the present invention, by setting a blade that can be moved horizontally inside the horizontal frame, can cut off the mycelium wrapped around the outside of the movable frame, so as to release the adhesion between the mycelium frame and the movable frame, and the mycelium frame can be easily removed. The operation is convenient and labor-saving, highly functional, and easy to promote and use.

[0016] The system includes a separation structure, a cutting structure, and a mushroom basket. Rotating the handle drives a rotating rod, which in turn drives a first worm and a second worm. The first worm meshes with a first worm wheel. As the rotating rod rotates, it drives a rotating shaft via the first worm and the first worm wheel. The surface of the rotating shaft is staggered with semi-circular abutment blocks, with adjacent sets of semi-circular abutment blocks arranged symmetrically. The rotating shaft passes through a fixed frame, causing the semi-circular abutment blocks to rotate within the fixed frame. The semi-circular abutment blocks are staggered on the rotating shaft, and adjacent sets of semi-circular abutment blocks... The components are symmetrically arranged, so when the rotating shaft rotates, adjacent sets of semicircular abutment blocks and concave surfaces alternately contact the bottom surface of the movable frame. The thickness of the concave surface is less than the thickness of the semicircular abutment block. When the edge of a semicircular abutment block contacts the movable frame, the edges of all semicircular abutment blocks contact the movable frame, and all movable frames are at their highest point. When the middle of a semicircular abutment block contacts the movable frame, the middle of the concave surface of the adjacent semicircular abutment block contacts the movable frame, and the movable frame contacting the middle of the concave surface is at its lowest point. This causes adjacent sets of movable frames to alternately undulate, and because of the mushroom frame... The bottom surface of the frame contacts multiple sets of movable frames. Therefore, when some movable frames descend, the remaining movable frames are at their highest point, ensuring stable support for the mushroom frame. When the movable frames move downwards away from the mushroom frame, since the height of the mushroom frame and the fixed frame remains unchanged, the movement of the movable frames between the fixed frame and the mushroom frame will not pull on the mycelium, and a gap can be left between the movable frames and the mushroom frame. The second worm gear meshes with the second worm wheel. When the rotating rod rotates, it drives the threaded rod to rotate through the second worm gear and the second worm wheel. Since the threaded rod passes through the movable frame and is threadedly connected to the movable frame, the movable frame... The end is slidably mounted on the guide rod, so the rotation of the threaded rod drives the moving frame to move within the movable slot. When the moving frame moves, it drives the blade to move horizontally. The blade is located between the fungal frame and the movable frame at the lowest point. Therefore, the movement of the blade can cut the mycelium between the fixed frame and the fungal frame, so that the fungal frame is separated from the separation structure. This makes it easy to remove the fungal frame from the separation structure. The cut mycelium will fall downwards onto the top surface of the collection frame and move forward along the inner wall of the collection frame, which is convenient for staff to process the mycelium uniformly. The operation is convenient and labor-saving, highly functional, and easy to promote and use. Attached Figure Description

[0017] Figure 1 This is a front view schematic diagram of the structure of the present invention; Figure 2 This is a front view schematic diagram of the movable frame structure of the present invention; Figure 3 This is an exploded view of the structure of the present invention; Figure 4 This is a side view schematic diagram of the structure of the first worm gear and the rotating rod of the present invention; Figure 5 This is an exploded view of the structure of the fixed frame and the movable frame of the present invention; Figure 6 This is a side sectional view of the structure of the movable frame of the present invention; Figure 7 This is a top sectional view of the cross frame structure of the present invention; Figure 8 This is a schematic diagram of the semi-circular abutment block and concave surface of the present invention; Figure 9 This is a schematic diagram of the semi-circular abutment block and concave surface of the movable frame of the present invention in its normal state; Figure 10 This is a schematic diagram of the semi-circular abutment block and concave surface when the movable frame of the present invention is in the movable state; Figure 11 This is a schematic diagram of the semi-circular abutment block and concave surface of the present invention; Figure 12 This is a schematic diagram showing the position and height structure of the fixed frame, movable frame, and blade of the present invention.

[0018] In the diagram: 1. Vertical frame; 11. Horizontal frame; 12. Movable groove; 13. Collection frame; 2. Separation structure; 21. Fixed frame; 22. Guide cylinder; 23. Rotating shaft; 24. Semi-circular abutment block; 25. Concave surface; 26. First worm gear; 27. Mounting frame; 28. Rotating rod; 29. ​​First worm; 210. Second worm; 211. Handle; 212. Movable frame; 213. Guide column; 214. Empty groove; 3. Cutting structure; 31. Threaded rod; 32. Second worm gear; 33. Guide rod; 34. Moving frame; 35. Blade; 4. Mushroom frame. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figures 1-12 One embodiment provided by the present invention: The vertical frame 1, horizontal frame 11, and mushroom basket 4 used in this application are products that can be purchased directly from the market. Their principles and connection methods are existing technologies well known to those skilled in the art, so they will not be described in detail here.

[0021] A device for cultivating filamentous fungal hyphae and its control system, comprising: a vertical frame 1, and two sets of horizontal frames 11 installed on the side wall of the vertical frame 1, the two sets of horizontal frames 11 being symmetrically installed front and back; A separation structure 2 is installed on the vertical frame 1, a cutting structure 3 is installed on the horizontal frame 11, and a mushroom basket 4 is placed on the separation structure 2; The inner wall of the horizontal frame 11 is provided with a movable groove 12, and the inner wall of the vertical frame 1 is fixedly installed with a collection frame 13. The separation structure 2 includes a fixed frame 21, which is fixedly installed on the inner wall of the horizontal frame 11. Guide cylinders 22 are fixedly installed at the front and rear ends of the inner side of the fixed frame 21. Rotating shafts 23 are inserted into the left and right sides of the fixed frame 21. A semi-circular abutment block 24 is fixedly installed in the middle of the rotating shaft 23. A concave surface 25 is opened on the surface of the semi-circular abutment block 24. A first worm gear 26 is fixedly installed at the right end of the rotating shaft 23. An installation frame 27 is fixedly installed at the right end of the inner side of the vertical frame 1. A rotating rod 28 is movably installed on the inner side of the installation frame 27 through a bearing. A first worm gear 29 is fixedly installed in the middle of the rotating rod 28. A second worm gear 210 is fixedly installed at the rear end of the rotating rod 28. A handle 211 is fixedly installed at the front end of the rotating rod 28. A movable frame 212 is movably installed on the top surface of the fixed frame 21. Guide columns 213 are fixedly connected to the front and rear ends of the bottom surface of the movable frame 212. Hollow slots 214 are opened on the left and right side walls of the movable frame 212. The cutting structure 3 includes a threaded rod 31, which is movably mounted on the inner side of a rear set of crossbeams 11 via bearings. A second worm gear 32 is fixedly connected to the right end of the threaded rod 31. A guide rod 33 is fixedly mounted on the inner side of a front set of crossbeams 11. A movable frame 34 is slidably mounted on the guide rod 33. A blade 35 is provided in the middle of the movable frame 34. By providing a movable blade 35 on the inner side of the crossbeams 11, the mycelium wrapped around the outside of the movable frame 212 can be cut off to release the adhesion between the mycelium frame 4 and the movable frame 212, so that the mycelium frame 4 can be easily removed. The operation is convenient and labor-saving, highly functional, and easy to promote and use.

[0022] Furthermore, the collection frame 13 is located on the lower side of the separation structure 2 and is installed at an angle. The collection frame 13 can collect the cut hyphae on the lower side, avoiding the hyphae from being scattered randomly, difficult to clean, rotting and deteriorating, and contaminating the equipment.

[0023] Furthermore, the fixed frame 21 is perpendicular to the rotating shaft 23. The semi-circular abutment block 24 is located inside the fixed frame 21. The semi-circular abutment block 24 corresponds one-to-one with the fixed frame 21. The arc length of the semi-circular abutment block 24 is greater than half of the whole. The semi-circular abutment block 24 can rotate stably between the fixed frame 21 and the movable frame 212. When the semi-circular abutment block 24 and the concave surface 25 drive the movable frame 212 to move up and down, the contact time between the semi-circular abutment block 24 and the movable frame 212 is longer than the contact time between the concave surface 25 and the movable frame 212. This makes the movable frame 212 stay at the highest point for a longer time, which is convenient for stable support of the mushroom frame 4.

[0024] Furthermore, the thickness of the semicircular abutment block 24 is greater than the thickness of the concave surface 25. The two adjacent sets of semicircular abutment blocks 24 are symmetrically arranged front and back. The arc edge of the semicircular abutment block 24 is in contact with the bottom surface of the movable frame 212. When the rotating shaft 23 rotates, it can drive the two adjacent sets of movable frames 212 to alternately rise and fall under the action of the semicircular abutment block 24 and the concave surface 25.

[0025] Furthermore, the first worm gear 26 meshes with the first worm 29, and the second worm 210 meshes with the second worm gear 32. The rotation of the first worm 29 can drive the rotating shaft 23 to rotate through the first worm gear 26, and the rotation of the second worm 210 can drive the threaded rod 31 to rotate through the second worm gear 32.

[0026] Furthermore, the threaded rod 31 passes through the rear end of the movable frame 34 and is threadedly connected to the movable frame 34. The movable frame 34 protrudes from the inside of the cross frame 11 through the movable slot 12. The rotation of the threaded rod 31 can drive the movable frame 34 to move horizontally within the cross frame 11.

[0027] Furthermore, the blade 35 is located inside the right side of the vertical frame 1, and above the fixed frame 21. The blade 35 is perpendicular to the rotation axis 23. When the movable frame 212 descends to its lowest point, the blade 35 is located above the movable frame 212. At this time, the blade 35 can pass over the movable frame 212 from above to cut the mycelium between the movable frame 212 and the fungal frame 4, providing conditions for the blade 35 to move inside the vertical frame 1.

[0028] A culture control system for filamentous fungal hyphae: S1. Basic Culture Medium Formulation PDA medium (most commonly used): Potato starch: 4-6 g / L • Glucose: 20 g / L • Agar: 15 g / L (solid) Sterilize at 121℃ for 20 minutes. S2. Cultivation Steps Solid culture: 1. Inoculation: Use an inoculation needle to pick up spores or mycelial blocks and spot or streak them onto a plate. 2. Incubation: Incubate upside down at 25-28℃ to avoid condensation. 3. Time: 3-7 days until the mycelium fully covers the plate. Liquid culture: 1. Inoculation: Add spore suspension at an inoculation rate of 5-10%. 2. Conditions: 25-28℃, shake culture at 150-200 rpm. 3. Time: 2-5 days, the mycelium grows in a flocculent manner. S3. Key Parameters • Temperature: 25-30℃ (most bacterial species) pH: 5.5-6.5 • Humidity: >85% (solid culture) • Lighting: Dark or dim light S4. Precautions 1. Strict aseptic technique 2. Regularly observe the morphology and color of the mycelium. 3. Transfer in a timely manner to prevent spore aging. 4. Ensure adequate aeration during liquid culture. S5. Frequently Asked Questions • Contamination: Strengthen sterilization and operational procedures Slow growth: Check temperature, nutrients, and pH. • Sparse hyphae: Optimize carbon-nitrogen ratio and trace elements Storage: Short-term storage at 4°C; long-term storage at -80°C for glycerol tubes. Working principle: During cultivation, multiple spawn bags are placed in the same spawn frame 4, and the spawn frame 4 is placed on the top surface of the movable frame 212. At this time, the bottom surface of the movable frame 212 is in contact with the arc edge of the semi-circular abutment block 24, so the top surfaces of multiple movable frames 212 remain flush. The movable frame 212 can stably support the spawn frame 4 from below, so that the spawn frame 4 is neatly arranged. As the cultivation time increases, mycelium will extend from the spawn bags, and since the spawn frame 4 has a hollow structure, some mycelium will extend downward from the hollow part and wrap around the outside of the movable frame 212 and the fixed frame 21. When it is necessary to remove the mycelium frame 4, the removal operation will be obstructed because the mycelium is wrapped around the outside of the movable frame 212 and the fixed frame 21. At this time, the handle 211 can be rotated to drive the rotating rod 28 to rotate. The rotating rod 28 drives the first worm 29 and the second worm 210 to rotate. The first worm 29 meshes with the first worm wheel 26. When the rotating rod 28 rotates, it drives the rotating shaft 23 to rotate through the first worm 29 and the first worm wheel 26. The surface of the rotating shaft 23 is staggered with semi-circular abutment blocks 24, and adjacent sets of semi-circular abutment blocks 24 are symmetrically arranged. At the same time, the rotating shaft 23 passes through the fixed frame 21. The rotating shaft 23 drives the semi-circular abutment blocks 24 to rotate inside the fixed frame 21. The semi-circular abutment blocks 24 are staggered on the rotating shaft 23, and adjacent sets of semi-circular abutment blocks 24 are symmetrically arranged. Therefore, the rotation... When the shaft 23 rotates, the two adjacent sets of semicircular abutment blocks 24 and concave surfaces 25 will alternately contact the bottom surface of the movable frame 212, and the thickness of the concave surface 25 is less than the thickness of the semicircular abutment block 24. When the edge of the semicircular abutment block 24 contacts the movable frame 212, the edges of all the semicircular abutment blocks 24 are in contact with the movable frame 212, and at this time all the movable frames 212 are at the highest point. When the middle of the semicircular abutment block 24 contacts the movable frame 212, the middle of the concave surface 25 on the adjacent semicircular abutment block 24 contacts the movable frame 212, and the movable frame 212 in contact with the middle of the concave surface 25 is at the lowest point, so that the two adjacent sets of movable frames 212 alternately undulate. Since the bottom surface of the mushroom frame 4 is in contact with multiple sets of movable frames 212, when some movable frames 212 descend, the remaining movable frames 212 are at the highest point, which can ensure stable support for the mushroom frame 4. When the movable frame 212 moves downward away from the fungal frame 4, since the height of the fungal frame 4 and the fixed frame 21 remains unchanged, the movement of the movable frame 212 between the fixed frame 21 and the fungal frame 4 will not pull on the mycelium, and a distance can be left between the movable frame 212 and the fungal frame 4. The second worm 210 meshes with the second worm wheel 32. When the rotating rod 28 rotates, it drives the threaded rod 31 to rotate through the second worm 210 and the second worm wheel 32. Since the threaded rod 31 passes through the movable frame 34 and is threadedly connected to the movable frame 34, and one end of the movable frame 34 is slidably mounted on the guide rod 33, the threaded rod 31... The rotation of the blade causes the movable frame 34 to move within the movable slot 12. As the movable frame 34 moves, it causes the blade 35 to move horizontally. The blade 35 is located between the fungal frame 4 and the movable frame 212 at its lowest point. Therefore, the movement of the blade 35 can cut the mycelium between the fixed frame 21 and the fungal frame 4, so that the fungal frame 4 can be separated from the separation structure 2. This makes it easier to remove the fungal frame 4 from the separation structure 2. The cut mycelium will fall downwards onto the top surface of the collection frame 13 and move forward along the inner wall of the collection frame 13, making it easier for staff to uniformly process the mycelium. The operation is convenient and labor-saving, highly functional, and easy to promote and use.

[0029] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Those skilled in the art can readily implement the present invention based on the accompanying drawings and the above description. However, any modifications, alterations, or variations made by those skilled in the art without departing from the scope of the present invention, utilizing the disclosed technical content, are equivalent embodiments of the present invention. Furthermore, any modifications, alterations, or variations made to the above embodiments based on the essential technology of the present invention are still within the protection scope of the present invention.

Claims

1. A device for cultivating filamentous fungal hyphae, comprising: A vertical frame (1) is provided, and two sets of horizontal frames (11) are installed on the side wall of the vertical frame (1), and the two sets of horizontal frames (11) are installed symmetrically in front and behind. Its features are: A separation structure (2) is installed on the vertical frame (1), a cutting structure (3) is installed on the horizontal frame (11), and a fungal frame (4) is placed on the separation structure (2). The inner wall of the horizontal frame (11) is provided with a movable groove (12), and a collection frame (13) is fixedly installed on the inner wall of the vertical frame (1). The separation structure (2) includes a fixing frame (21), which is fixedly installed on the inner wall of the cross frame (11). Guide cylinders (22) are fixedly installed at the front and rear ends of the inner side of the fixing frame (21). Rotating shafts (23) are inserted on the left and right sides of the fixing frame (21). A semi-circular abutment block (24) is fixedly installed in the middle of the rotating shaft (23). A concave surface (25) is opened on the surface of the semi-circular abutment block (24). A first worm gear (26) is fixedly installed at the right end of the rotating shaft (23). A mounting bracket is fixedly installed at the right end of the inner side of the vertical frame (1). 27), a rotating rod (28) is movably mounted on the inner side of the mounting frame (27) via a bearing. A first worm gear (29) is fixedly mounted in the middle of the rotating rod (28). A second worm gear (210) is fixedly mounted at the rear end of the rotating rod (28). A handle (211) is fixedly mounted at the front end of the rotating rod (28). A movable frame (212) is movably mounted on the top surface of the fixed frame (21). Guide columns (213) are fixedly connected to the front and rear ends of the bottom surface of the movable frame (212). Hollow slots (214) are opened on the left and right side walls of the movable frame (212). The cutting structure (3) includes a threaded rod (31), which is movably mounted on the inner side of a set of crossbeams (11) on the rear side via a bearing. A second worm gear (32) is fixedly connected to the right end of the threaded rod (31). A guide rod (33) is fixedly mounted on the inner side of a set of crossbeams (11) on the front side. A movable frame (34) is slidably mounted on the guide rod (33). A blade (35) is provided in the middle of the movable frame (34).

2. The device for cultivating filamentous fungal hyphae according to claim 1, characterized in that: The collection box (13) is located on the lower side of the separation structure (2) and is installed at an angle.

3. The device for cultivating filamentous fungal hyphae according to claim 1, characterized in that: The fixed frame (21) is perpendicular to the rotating shaft (23). The semi-circular abutment block (24) is located inside the fixed frame (21). The semi-circular abutment block (24) corresponds to the fixed frame (21) one by one. The arc length of the semi-circular abutment block (24) is greater than half of the whole.

4. The device for cultivating filamentous fungal hyphae according to claim 3, characterized in that: The thickness of the semicircular abutment block (24) is greater than the thickness of the concave surface (25). Two adjacent sets of semicircular abutment blocks (24) are symmetrically arranged front and back. The arc edge of the semicircular abutment block (24) is in contact with the bottom surface of the movable frame (212).

5. The device for cultivating filamentous fungal hyphae according to claim 1, characterized in that: The first worm wheel (26) meshes with the first worm (29), and the second worm (210) meshes with the second worm wheel (32).

6. The device for cultivating filamentous fungal hyphae according to claim 1, characterized in that: The threaded rod (31) passes through the rear end of the movable frame (34) and is threadedly connected to the movable frame (34), which is exposed from inside the cross frame (11) via the movable slot (12).

7. The device for cultivating filamentous fungal hyphae according to claim 1, characterized in that: The blade (35) is located inside the right side of the vertical frame (1), the blade (35) is located on the upper side of the fixed frame (21), and the blade (35) is perpendicular to the rotation axis (23).

8. A culture and control system for filamentous fungal hyphae according to any one of claims 1-7: S1. Basic Culture Medium Formulation PDA medium (most commonly used): Potato starch: 4-6 g / L • Glucose: 20 g / L • Agar: 15 g / L (solid) Sterilize at 121℃ for 20 minutes. S2. Cultivation Steps Solid culture:

1. Inoculation: Use an inoculation needle to pick up spores or mycelial blocks and spot or streak them onto a plate.

2. Incubation: Incubate upside down at 25-28℃ to avoid condensation.

3. Time: 3-7 days until the mycelium fully covers the plate. Liquid culture:

1. Inoculation: Add spore suspension at an inoculation rate of 5-10%.

2. Conditions: 25-28℃, shake culture at 150-200 rpm.

3. Time: 2-5 days, the mycelium grows in a flocculent manner. S3. Key Parameters • Temperature: 25-30℃ (most bacterial species) pH: 5.5-6.5 • Humidity: >85% (solid culture) • Lighting: Dark or dim light S4. Precautions 1. Strict aseptic technique 2. Regularly observe the morphology and color of the mycelium.

3. Transfer in a timely manner to prevent spore aging.

4. Ensure adequate aeration during liquid culture. S5. Frequently Asked Questions • Contamination: Strengthen sterilization and operational procedures Slow growth: Check temperature, nutrients, and pH. • Sparse hyphae: Optimize carbon-nitrogen ratio and trace elements Storage: Store at 4℃ for short periods, or at -80℃ for long periods in glycerol tubes.

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