Fungicide for improving transplanting survival rate of xanthoceras sorbifolia bunge and preparation method thereof

Abstract

The invention relates to a microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge and a preparation method thereof.The microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge is characterized by comprising trichoderma harzianum, yeast cell walls and organic matter, and the organic matter contains monosaccharide or polysaccharide capable of being utilized by the trichoderma harzianum. The transplanting survival rate of xanthoceras sorbifolia bunge can be increased.

Description

Technical Field

[0001] This invention relates to a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* and its preparation method. Background Technology

[0002] Xanthoceras sorbifolium Bunge is a rare woody oilseed plant and an excellent species for soil and water conservation, sand control, and ornamental purposes. It possesses excellent characteristics such as salt and alkali tolerance, drought resistance, and tolerance to poor soil conditions, and is mainly distributed in arid and semi-arid regions. With its beautiful tree shape, vibrant flowers, and high oil content in its kernels, Xanthoceras sorbifolium Bunge has medicinal uses for its flowers, fruit shells, and fruit stalks, making it highly valuable economically, socially, and ecologically.

[0003] Located in Northwest China, Gansu is mostly arid and semi-arid. Its unique climate has created unique landforms, such as grottoes and stone forests, which have high sightseeing and tourism value.

[0004] However, due to the influence of climate and planting methods, the soil suffers from desertification and salinization, with a pH level between 7.8 and 8.5. This means that some soils are no longer suitable for planting, and even if planting is forced, the harvest is rarely commensurate with the labor invested.

[0005] Due to its ornamental value and its ability to adapt to the harsh climate of Northwest China and maintain and improve the soil, the Chinese pistache tree is now widely cultivated in the region.

[0006] However, due to the harsh climate in Northwest China, even plants like *Xanthoceras sorbifolium* frequently die, necessitating transplantation. Because of the poor root healing ability of *Xanthoceras sorbifolium* and the saline-alkaline, high-pH soil of Northwest China, the survival rate of transplanted *Xanthoceras sorbifolium* trees is not high, around 80%. There is an urgent market need for products or methods to improve the survival rate of transplanted *Xanthoceras sorbifolium* trees. Summary of the Invention

[0007] This invention provides a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* and its preparation method, solving the technical problem of improving the survival rate of transplanted *Xanthoceras sorbifolium*.

[0008] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: A microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* comprises *Trichoderma harzianum*, yeast cell walls, organic matter, and a nitrogen source, wherein the organic matter contains monosaccharides or polysaccharides that can be utilized by *Trichoderma harzianum*.

[0009] It also includes a rooting agent; the rooting agent is one or more of sodium naphthaleneacetate, potassium indolebutyrate, and potassium indoleacetate; the organic matter is one or more of potassium humate, mushroom residue, and decomposed manure; the nitrogen source is one or more of potassium nitrate, urea, ammonium sulfate, and amino acids.

[0010] The mass ratio of Trichoderma harzianum, yeast cell wall, organic matter, nitrogen source and rooting agent is 2-10:10-50:50-80:3-6:0-0.1.

[0011] A method for preparing a microbial agent to improve the survival rate of transplanted *Xanthoceras sorbifolium* involves mixing *Trichoderma harzianum* and yeast cell walls to obtain product A; then mixing product A, organic matter, a nitrogen source, and a rooting agent to obtain the microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*; or Mix the organic matter, nitrogen source and rooting agent to obtain product B; Mixing product A and product B together yields a fungicide for improving the survival rate of transplanted *Sapindus mukorossi*.

[0012] The invention has the following beneficial technical effects: 1. This application proposes to prevent pathogen infection of the root system of *Xanthoceras sorbifolium* and improve the survival rate of *Xanthoceras sorbifolium* by adding *Trichoderma harzianum* and organic matter that can be utilized by *Trichoderma harzianum*.

[0013] 2. This application incorporates yeast cell walls. In the early stages, the three-dimensional network structure of the yeast cell walls adsorbs Trichoderma harzianum, thereby creating a population effect of Trichoderma harzianum, which can better control pathogens. At the same time, Trichoderma harzianum releases β-glucanase by absorbing organic matter, which degrades β-glucan in the yeast cell walls. The β-glucan produced enhances the plant's immunity and resistance, promotes better root growth, and further improves the survival rate of transplanted Xanthoceras sorbifolium.

[0014] 3. In terms of the preparation method, this application first mixes Trichoderma harzianum with yeast cell walls to prepare product A, and then mixes it with other substances. This avoids the problem of excessively high local concentrations of certain substances due to adsorption by yeast cell walls, which would affect the recovery of plant roots and reduce the transplant survival rate of Xanthoceras sorbifolium. Detailed Implementation

[0015] To better understand the above technical solutions, the technical solutions of the present invention will be described in detail below with reference to preferred examples. Example 1

[0016] A microbial agent for improving the survival rate of transplanted *Sapindus mukorossi* is composed of *Trichoderma harzianum*, yeast cell walls, organic matter, and a nitrogen source in a mass ratio of 4:15:69:12.

[0017] The organic matter is potassium humate; the nitrogen source is amino acid powder.

[0018] A method for preparing a microbial agent to improve the survival rate of transplanted *Xanthoceras sorbifolium* involves mixing *Trichoderma harzianum* and yeast cell walls to obtain product A; then mixing product A, organic matter, and a nitrogen source to obtain the microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*. Example 2

[0019] A microbial agent for improving the survival rate of transplanted *Sapindus mukorossi* is composed of *Trichoderma harzianum*, yeast cell walls, organic matter, nitrogen source, and rooting agent in a mass ratio of 4:15:69:12:0.03.

[0020] The organic matter is potassium humate; the nitrogen source is amino acid powder; and the rooting agent is potassium indolebutyrate.

[0021] A method for preparing a microbial agent to improve the survival rate of transplanted *Xanthoceras sorbifolium* is characterized by mixing *Trichoderma harzianum* and yeast cell walls to obtain product A; mixing product A, organic matter, nitrogen source, and rooting agent to obtain the microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*. Example 3

[0022] A microbial agent for improving the survival rate of transplanted *Sapindus mukorossi* is composed of *Trichoderma harzianum*, yeast cell walls, organic matter, nitrogen source, and rooting agent in a mass ratio of 4:15:69:12:0.03.

[0023] The organic matter is potassium humate; the nitrogen source is amino acid powder; and the rooting agent is potassium indolebutyrate.

[0024] A method for preparing a fungal agent to improve the survival rate of transplanted *Sapindus mukorossi* is characterized by mixing the cell walls of *Trichoderma harzianum* and yeast to obtain product A. Mix the organic matter, nitrogen source and rooting agent to obtain product B; Mixing product A and product B together yields a fungicide for improving the survival rate of transplanted *Sapindus mukorossi*. Example 4

[0025] A microbial agent for improving the survival rate of transplanted *Sapindus mukorossi* is composed of *Trichoderma harzianum*, yeast cell walls, organic matter, nitrogen source, and rooting agent in a mass ratio of 6:20:70:4:0.03.

[0026] The organic matter is a combination of mushroom residue and decomposed pig manure in a mass ratio of 2:1; the nitrogen source is a combination of ammonium sulfate and urea in a mass ratio of 3:1; and the rooting agent is sodium naphthaleneacetate.

[0027] A method for preparing a fungal agent to improve the survival rate of transplanted *Sapindus mukorossi* is characterized by mixing the cell walls of *Trichoderma harzianum* and yeast to obtain product A. Mix the organic matter, nitrogen source and rooting agent to obtain product B; Mixing product A and product B together yields a fungicide for improving the survival rate of transplanted *Sapindus mukorossi*. Example 5

[0028] A microbial agent for improving the survival rate of transplanted *Sapindus mukorossi* is composed of *Trichoderma harzianum*, yeast cell walls, organic matter, nitrogen source, and rooting agent in a mass ratio of 8:22:69:3:0.03.

[0029] The organic matter is well-rotted pig manure; the nitrogen source is urea; and the rooting agent is sodium naphthaleneacetate.

[0030] A method for preparing a fungal agent to improve the survival rate of transplanted *Sapindus mukorossi* is characterized by mixing the cell walls of *Trichoderma harzianum* and yeast to obtain product A. Mix the organic matter, nitrogen source and rooting agent to obtain product B; Mixing product A and product B together yields a fungicide for improving the survival rate of transplanted *Sapindus mukorossi*.

[0031] The Trichoderma harzianum used in the embodiments of this application is Trichoderma harzianum T-22.

[0032] The following experimental data further illustrates the beneficial effects of the present invention: Experiment 1 1.1 Experimental Site: Bao'an Village, Dongsheng Town, Jingyuan County, Baiyin City, Gansu Province; Soil conditions: pH 8.3, organic matter 0.26%, total nitrogen 0.35 mg / kg, available potassium 116.53 mg / kg, available phosphorus 6.9 mg / kg, total water-soluble salt content 0.26%. It is classified as slightly saline-alkali soil, which has already affected the growth of most crops.

[0033] 1.2 Experimental test: Survival rate (%) after 1 year.

[0034] 1.3 Test Materials: 3-year-old *Xanthoceras sorbifolium* seedlings; Comparison 1 (except for the absence of yeast cell walls, all other aspects were the same as in Example 1); Comparison 2 (except for the absence of *Trichoderma harzianum*, all other aspects were the same as in Example 1); Comparison 3 (except for the substitution of potassium humate with amino acid powder, all other aspects were the same as in Example 1); Comparison 4 (except for the substitution of amino acid powder with potassium humate, all other aspects were the same as in Example 1); Comparison 5 (except for the preparation method of mixing *Trichoderma harzianum*, yeast cell walls, organic matter, and nitrogen source, all other aspects were the same as in Example 1); Comparison 6 (except for the preparation method of mixing *Trichoderma harzianum*, yeast cell walls, and nitrogen source to obtain Product A; mixing Product A with organic matter to obtain the inoculant for improving the survival rate of transplanted *Xanthoceras sorbifolium*, all other aspects were the same as in Example 1). Examples 1 to 5 are identical to Example 1, Comparative 7 (except that the preparation method is to mix Trichoderma harzianum, yeast cell walls and organic matter to obtain product A; and mix product A with a nitrogen source to obtain a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*), Comparative 8 (except that the preparation method is to mix Trichoderma harzianum, yeast cell walls and rooting agent to obtain product A; and mix product A with organic matter and nitrogen source to obtain a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*), Comparative 9 (except that the preparation method is to mix Trichoderma harzianum, yeast cell walls, nitrogen source and rooting agent to obtain product A; and mix product A with organic matter to obtain a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*), Comparative 9 (except that the preparation method is to mix Trichoderma harzianum, yeast cell walls, nitrogen source and rooting agent to obtain product A; and mix product A with organic matter to obtain a fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*), Comparative 9.

[0035] 1.4 Experimental methods: Three-year-old seedlings with uniform growth vigor were transplanted on October 15, 2024, with a row spacing of 2*2m and 80 seedlings transplanted per treatment. The survival rate (%) was counted one year after transplanting.

[0036] Except for the different treatments at each location, all other treatments were the same in this experiment.

[0037] 2 Results and Analysis Table 1

[0038] The comparisons in Table 1 between Comparison 1 (all identical to Example 1 except for the absence of yeast cell walls), Comparison 2 (all identical to Example 1 except for the absence of *Trichoderma harzianum*), Comparison 3 (all identical to Example 1 except for the substitution of potassium humate with amino acid powder), and Comparison 4 (all identical to Example 1 except for the substitution of amino acid powder with potassium humate) and Example 1 show that the selection of yeast cell walls, *Trichoderma harzianum*, and organic matter all affect the effectiveness of the product. Comparison 3 (all identical to Example 1 except for the substitution of potassium humate with amino acid powder) may be due to the lack of a carbon source that *Trichoderma harzianum* can directly utilize, thus affecting the effectiveness of the product. Comparison 4 (all identical to Example 1 except for the substitution of amino acid powder with potassium humate) may be due to the lack of a nitrogen source required for the growth of *Trichoderma harzianum*, also affecting the effectiveness.

[0039] The comparison of data from Table 1 between Example 5 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls, organic matter, and nitrogen source, all other methods are the same as Example 1), Example 6 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls, and nitrogen source to obtain Product A; mixing Product A with organic matter to obtain the inoculant for improving the survival rate of transplanted Xanthoceras sorbifolium, all other methods are the same as Example 1), and Example 7 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls, and organic matter to obtain Product A; mixing Product A with nitrogen source to obtain the inoculant for improving the survival rate of transplanted Xanthoceras sorbifolium, all other methods are the same as Example 1) and Example 1 shows that the preparation method directly affects the application effect.

[0040] Comparison 5 (except for the preparation method of mixing Trichoderma harzianum, yeast cell wall, organic matter and nitrogen source, all other aspects are the same as in Example 1) may be that when the yeast cell wall is mixed, it cannot adsorb Trichoderma harzianum, which prevents Trichoderma harzianum from forming a population effect, thus affecting the use effect.

[0041] Comparison 6 (except for the preparation method of mixing Trichoderma harzianum, yeast cell wall and nitrogen source to obtain product A; mixing product A with organic matter to obtain the fungal agent for improving the survival rate of transplanted Xanthoceras sorbifolium, all other aspects are the same as in Example 1) may be that the high concentration of nitrogen source in yeast cell wall affected the growth of Trichoderma harzianum, thus affecting the effect of use.

[0042] Comparison 7 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls and organic matter to obtain product A; mixing product A with a nitrogen source to obtain a fungal agent for improving the survival rate of transplanted Xanthoceras sorbifolium, all other aspects are the same as in Example 1) may have affected the population effect of Trichoderma harzianum.

[0043] The comparison of data from Table 1 between Example 8 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls, and rooting agents to obtain Product A; mixing Product A, organic matter, and nitrogen source to obtain the microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*, all other methods are consistent with Example 2), Example 9 (except for the preparation method of mixing Trichoderma harzianum, yeast cell walls, nitrogen source, and rooting agents to obtain Product A; mixing Product A with organic matter to obtain the microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium*, all other methods are consistent with Example 2), and Example 2 shows that the rooting agent and the way the rooting agent and nitrogen source are added also affect the effect. Adding the rooting agent to Trichoderma harzianum and yeast cell walls may cause the yeast cell walls to adsorb the rooting agent, resulting in an excessively high local concentration in the soil, which leads to premature root aging and thus affects the survival rate.

Claims

1. A fungal agent for improving the survival rate of transplanted *Sapindus mukorossi*, characterized in that, It includes Trichoderma harzianum, yeast cell walls, organic matter, and a nitrogen source, wherein the organic matter contains monosaccharides or polysaccharides that can be utilized by Trichoderma harzianum.

2. The microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* as described in claim 1, characterized in that, It also includes a rooting agent; the rooting agent is one or more of sodium naphthaleneacetate, potassium indolebutyrate, and potassium indoleacetate; the organic matter is one or more of potassium humate, mushroom residue, and decomposed manure; the nitrogen source is one or more of potassium nitrate, urea, ammonium sulfate, and amino acids.

3. The microbial agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* as described in claim 1, characterized in that, The mass ratio of Trichoderma harzianum, yeast cell wall, organic matter, nitrogen source and rooting agent is 2-10:10-50:50-80:3-6:0-0.

1.

4. The method for preparing the fungal agent for improving the survival rate of transplanted *Xanthoceras sorbifolium* as described in claim 1, characterized in that, Mixing the cell walls of *Trichoderma harzianum* and yeast yields product A; mixing product A, organic matter, a nitrogen source, and a rooting agent yields a microbial agent for improving the survival rate of transplanted *Sapindus mukorossi*; or Mix the organic matter, nitrogen source and rooting agent to obtain product B; Mixing product A and product B together yields a fungicide for improving the survival rate of transplanted *Sapindus mukorossi*.

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