High-throughput pipetting plate making system

Abstract

The invention relates to a high throughput pipetting plate making system. The high-throughput pipetting plate making system comprises a pipetting head module, a pipetting device cleaning device, a pipetting arm and a stacking device. Wherein the pipetting head module comprises a pipetting mechanism, a liquid sucking and spraying driving mechanism and pipetting head quick-changing equipment, and the pipetting mechanism is detachably connected with the liquid sucking and spraying driving mechanism through the pipetting head quick-changing equipment; the pipetting device cleaning equipment comprises a plurality of mutually independent cleaning channels, and at least one pipetting suction head extends into each cleaning channel; the pipetting arm drives the pipetting head module to move to a preset working position; wherein the preset working positions comprise a sample plate liquid absorption position, a reaction plate liquid spraying position and a cleaning position; the stacking device is used for storing consumables and carrying out consumable interaction on the consumables and the pipette head module. The system can be flexibly used in a multi-flux manner, is high in efficiency, is not liable to generate cross contamination, automatically and interactively takes and places consumables, improves the consumable feeding efficiency, improves the plate making efficiency, and guarantees the pipetting precision.

Description

Technical Field

[0001] This application relates to the field of pipetting technology, and in particular to high-throughput pipetting systems. Background Technology

[0002] In fields such as biological experiments and medical testing, high-throughput liquid handling platforms are the core equipment for reaction plate preparation. The pipetting system is responsible for transferring samples from the sample plate to the reaction plate. As a key execution component, its performance directly affects the plate preparation efficiency, accuracy, and reliability of experimental results.

[0003] Currently, the throughput of existing pipetting and plate-making equipment in China is limited. Most equipment features a fixed 96-channel design, resulting in slow plate-making speeds and failing to meet the high-efficiency requirements of large-scale experiments. Their pipetting performance is also insufficient, with a minimum dispensing volume of only 0.8 μL and a small aspiration volume, making them unsuitable for the precise pipetting needs of micro-volume experiments. Secondly, the loading efficiency of sample plates and reaction plates, as core consumables, is a key factor restricting the overall efficiency of the pipetting and plate-making system. Existing pipetting and plate-making equipment has low loading and unloading efficiency, and frequent refilling during high-throughput plate-making can easily interrupt the process. The low level of automation in loading, relying on manual labor, also makes it prone to consumable positioning errors due to manual operation, affecting pipetting accuracy. Furthermore, poor coordination and disconnect between transport rhythm and pipetting progress severely hinder plate-making efficiency. In addition, the cleaning structure design of existing pipetting and plate-making equipment is unreasonable. Most existing cleaning tanks are single-water tank structures, with all pipetting tips being cleaned together, which easily leads to cross-contamination between channels. Furthermore, as core consumables, the loading efficiency of sample plates and reaction plates is a key factor restricting the overall efficiency of the pipetting plate preparation system. Existing pipetting plate preparation equipment has low loading and unloading efficiency, and frequent material replenishment during high-throughput plate preparation can easily interrupt the plate preparation process. The degree of automation in loading is low, relying on manual labor, and manual operation can easily lead to consumable positioning deviations, affecting pipetting accuracy. The coordination between the equipment is poor, and the transfer rhythm is out of sync with the pipetting progress, which seriously drags down the plate preparation efficiency. Summary of the Invention

[0004] Based on this, a high-throughput pipetting system is provided, which has the problems of not being able to be used flexibly at multiple throughputs, low efficiency in material feeding, low plate making efficiency, and easy cross-contamination.

[0005] Embodiments of this application propose a high-throughput pipetting system, comprising:

[0006] A pipette module includes a pipetting mechanism, a suction-and-spray driving mechanism, and a quick-change pipette tip device. The pipetting mechanism is provided with a pipette tip connection part. The pipetting mechanism is detachably connected to the suction-and-spray driving mechanism through the quick-change pipette tip device. The suction-and-spray driving mechanism drives the pipetting mechanism to provide positive or negative pressure to the pipette tip connection part.

[0007] A pipette cleaning device includes a water inlet mechanism, a first drainage mechanism, and several independent cleaning channels. The water inlet mechanism is connected to the cleaning channels to input cleaning solution, and the first drainage mechanism is connected to the cleaning channels to discharge the cleaning solution. Each cleaning channel allows at least one pipette tip to be inserted.

[0008] A pipette arm, driven and connected to the pipette head module, is used to drive the pipette head module to move to a preset working position; wherein, the preset working position includes a sample plate aspiration position, a reaction plate spray position, and a washing position;

[0009] A stack device for storing consumables and for interacting with the pipette tip module.

[0010] In one embodiment, the stacking device includes:

[0011] A hierarchical stack module includes several independent storage spaces, configured to independently access the consumables within any of the independent storage spaces; the consumables include sample boards;

[0012] A stackable module, including stacked storage space, is configured to sequentially access consumables within the stacked storage space in a first-in-first-out (FIFO) order; the consumables include reaction plates.

[0013] In one embodiment, the hierarchical stack module includes:

[0014] A tiered storage unit is provided with several layers of independent storage spaces arranged vertically for storing the consumables in layers.

[0015] A location identification unit is used to acquire the identification information and first location information of the consumable, as well as the type information of the hierarchical storage unit;

[0016] The handling unit is communicatively connected to the position detection unit and is used to retrieve the consumable from the independent storage space at the corresponding position according to the first position information, or to put the consumable back into the independent storage space at the corresponding position.

[0017] In one embodiment, the transport unit includes:

[0018] Lifting assembly, used to provide lifting driving force;

[0019] A rotating component, mounted on the lifting component, is used to provide rotational movement about a preset rotation axis; multiple hierarchical storage units are provided and arranged circumferentially along the preset rotation axis;

[0020] A telescopic pick-and-place assembly, mounted on the rotating assembly, is used to provide telescopic movement in the horizontal direction to pick up and place the consumables in the independent storage space.

[0021] In one embodiment, the lifting assembly is provided with a counterweight mechanism, which is connected to the load end of the lifting assembly to balance the load of the lifting assembly.

[0022] And / or, the telescopic take-up and drop-down assembly is provided with an in-situ detection unit for detecting whether the consumable exists in the independent storage space on the telescopic take-up and drop-down assembly and at the corresponding position.

[0023] In one embodiment, the stacked module includes:

[0024] A stacking storage unit is provided, wherein the stacking storage space is provided for stacking multiple of the consumables in a vertical direction;

[0025] A transfer unit is used to reciprocate horizontally below the stacked storage unit to feed or remove the consumables into or from the stacked storage unit.

[0026] The lifting unit, located below the transfer unit, is used to move vertically to cooperate with the stacked storage unit, to release the consumables at the bottom of the stacked storage unit onto the transfer unit, or to lift and store the consumables on the transfer unit into the stacked storage space.

[0027] In one embodiment, the stacked storage unit includes:

[0028] A latching mechanism is provided at the bottom of the stacked storage space; the latching mechanism includes a rotatable latching member and a latching drive assembly driven by the latching member. The latching member is used to abut against the consumables at the bottom of the stacked storage space and store them in the stacked storage space. The latching drive assembly drives the latching member to rotate, so as to drive the latching member to abut against or release the consumables.

[0029] In one embodiment, the latching drive assembly includes:

[0030] An electromagnetic drive component is driven and connected to the latching component, and is used to drive the latching component to rotate when energized; a latching reset component is driven and connected to the latching component, and is used to provide a reset driving force for the latching component after the electromagnetic drive component is de-energized.

[0031] In one embodiment, the lifting unit includes:

[0032] The lifting tray is used to feed the consumables into the stacked storage unit along the direction of the transfer unit. The lifting tray is provided with a first side and a second side in sequence, and the top height of the second side is higher than the top height of the first side.

[0033] A lifting drive assembly, connected to the lifting pallet drive, is used to drive the lifting pallet to rise and fall to the plate picking station and the positioning station;

[0034] In the board picking station, the first and second guard edges together abut against the consumable material at the bottom of the stacked storage unit to support the consumable material.

[0035] At the positioning station, the second stop is used to stop and limit the horizontal movement of the consumable on the transfer unit, while the first stop does not interfere with the horizontal movement of the consumable, so that the consumable is positioned at a preset interactive position for feeding the consumable into the stacked storage unit.

[0036] In one embodiment, the high-throughput pipetting system further includes a material transport module for transporting the consumables;

[0037] The logistics transmission module includes:

[0038] The sample plate conveying mechanism is used to interact with the layered stacking module for consumables and to convey the sample plate to the sample plate aspiration position.

[0039] The reaction plate conveying mechanism is used to interact with the stacked module for consumables, and to convey the reaction plate to the reaction plate spraying position or the next station.

[0040] According to the high-throughput pipetting system of this application embodiment, the pipetting arm drives the pipetting head module to move automatically. The quick-change device for the pipetting head detachably connects the pipetting mechanism to the aspiration-spraying liquid drive mechanism, allowing the pipetting mechanism to be separated from the aspiration-spraying liquid drive mechanism. The aspiration-spraying liquid drive mechanism can connect to pipetting mechanisms with different channels, such as 96 channels and 384 channels, to adapt to the pipetting needs of different experimental scenarios such as high throughput and low throughput, without the need to configure a complete set of equipment separately for different throughputs; or pipetting mechanisms of different volumes, covering various specifications such as 10ul, 30ul, 50ul, 200ul and 1000ul, which can meet the liquid transfer operations of micro-volume, regular volume and even large range, and adapt to the accuracy and range requirements of different experiments; or pipetting mechanisms of different specifications and types of consumables from different manufacturers. With the above setup, since the aspiration and spraying liquid drive mechanism is reusable, only different specifications of pipetting mechanisms need to be replaced to achieve the functions of different specifications of pipettes. There is no need to purchase a complete set of pipetting equipment separately for each pipetting need, which greatly reduces the overall procurement cost of the equipment. At the same time, in the maintenance process, faulty pipetting mechanisms can be disassembled, repaired or replaced individually without stopping the entire set of equipment for maintenance. Experimenters can quickly replace the corresponding specifications of pipetting mechanisms according to real-time experimental needs without having to readjust the power system of the entire set of equipment. This allows for flexible switching of experimental scenarios, reduces maintenance costs and equipment downtime, and improves experimental efficiency.

[0041] Secondly, the pipetting device cleaning equipment in the high-throughput pipetting system of this application embodiment can clean the pipetting tips. During the cleaning process, the water inlet mechanism delivers cleaning solution to the cleaning channel, where the cleaning solution cleans the workpiece. The cleaning solution in the cleaning channel is then discharged through the first drainage mechanism. When the workpiece to be cleaned is configured as a pipetting tip, the aspiration and spraying liquid driving mechanism drives the pipetting tip to move, causing each pipetting tip to be inserted into its corresponding independent cleaning channel. By simulating the repeated aspiration and dispensing of cleaning solution through the pipetting tip, the cleaning solution fully contacts the inner and outer walls of the pipetting tip, removing residual liquid, impurities, or contaminants from the pipetting tip, thus achieving a cleaning effect. Since the cleaning channels corresponding to each pipetting tip are independent, the problem of cross-contamination between pipetting tips can be avoided, ensuring cleaning cleanliness and improving the accuracy of experimental or testing results.

[0042] Furthermore, the stacking device in the high-throughput pipetting system of this application embodiment has a large storage capacity for consumables, reducing the frequency of replenishment and avoiding process interruptions; through automated interactive picking and placing of consumables, it eliminates reliance on manual labor, solves the positioning deviation problem caused by manual operation, improves consumable feeding efficiency and plate making efficiency, while ensuring pipetting accuracy. Attached Figure Description

[0043] Figure 1 This is a schematic diagram of the structure of a high-throughput pipetting plate-making system according to an embodiment of this application.

[0044] Figure 2 This is a schematic diagram of the structure of a high-throughput pipetting plate-making system according to another embodiment of this application.

[0045] Figure 3 This is a schematic diagram of the structure of a layered stack module in a high-throughput pipetting system according to an embodiment of this application.

[0046] Figure 4 This is a schematic diagram of the transport unit of a layered stack module in a high-throughput pipetting system according to an embodiment of this application.

[0047] Figure 5 This is a schematic diagram of the structure of a rotating component in a high-throughput pipetting system according to an embodiment of this application.

[0048] Figure 6 This is a schematic diagram of the structure of a telescopic pick-and-place component in a high-throughput pipetting system according to an embodiment of this application.

[0049] Figure 7 This is a schematic diagram of the stacked module in a high-throughput pipetting system according to an embodiment of this application.

[0050] Figure 8 This is a partial cross-sectional view of a stacked module in a high-throughput pipetting system according to an embodiment of this application.

[0051] Figure 9 This is a schematic diagram of the transfer unit of a stacked module in a high-throughput pipetting system according to an embodiment of this application.

[0052] Figure 10 This is a partial structural diagram of the docking hole in a high-throughput pipetting system according to an embodiment of this application.

[0053] Figure 11 This is a schematic diagram of the lifting unit of a stacked module in a high-throughput pipetting system according to an embodiment of this application.

[0054] Figure 12 This is a schematic diagram of the overall structure of a high-throughput pipetting and plating system according to an embodiment of this application. Figure 1 .

[0055] Figure 13 This is a schematic diagram of the overall structure of a high-throughput pipetting and plating system according to an embodiment of this application. Figure 2 .

[0056] Figure 14 This is a top view of a high-throughput pipetting system according to an embodiment of this application.

[0057] Figure 15This is a schematic diagram illustrating the liquid aspiration and spraying drive mechanism in a pipette module according to an embodiment of this application.

[0058] Figure 16 This is a schematic diagram of the structure of a pipette tip module according to an embodiment of this application.

[0059] Figure 17 This is a cross-sectional view showing the piston shaft assembly structure in a pipette module according to an embodiment of this application.

[0060] Figure 18 This is a cross-sectional view of the pipetting mechanism in a pipetting head module according to an embodiment of this application.

[0061] Figure 19 for Figure 18 A magnified view of a portion of point A in the middle.

[0062] Figure 20 This is an exploded view of the piston shaft assembly in a pipette module according to an embodiment of this application.

[0063] Figure 21 This is a schematic diagram of the retractor mechanism after resetting in a pipette head module according to an embodiment of this application.

[0064] Figure 22 This is a schematic diagram of the pipette tip retraction mechanism in an embodiment of this application during the retraction process.

[0065] Figure 23 This is a schematic diagram of the pipette tip support assembly in a high-throughput pipetting system according to an embodiment of this application.

[0066] Figure 24 This is a schematic diagram of the structure of a quick-change pipette tip device according to an embodiment of this application.

[0067] Figure 25 This is a partial structural schematic diagram of a quick-change pipette tip device according to an embodiment of this application.

[0068] Figure 26 This is a front view of a quick-change pipette tip device according to an embodiment of this application.

[0069] Figure 27 for Figure 26 Sectional view at point PP.

[0070] Figure 28 This is a schematic diagram of the pipetting mechanism in a pipetting device according to an embodiment of this application.

[0071] Figure 29 This is a schematic diagram of the structure of a pipetting device cleaning apparatus according to an embodiment of this application.

[0072] Figure 30 This is a cross-sectional view of a pipetting apparatus cleaning device according to an embodiment of this application.

[0073] Figure 31 This is an exploded view of the cleaning equipment of a pipetting apparatus according to an embodiment of this application after it has been flipped over.

[0074] Figure 32 This is a cross-sectional view showing the cleaning channel in a pipetting apparatus cleaning device according to an embodiment of this application.

[0075] Figure 33 This is a schematic diagram of an automatic water inlet control mechanism in a pipette cleaning device according to an embodiment of this application.

[0076] Figure 34 This is a schematic diagram illustrating the structure of the frame module in a high-throughput pipetting system according to an embodiment of this application.

[0077] Figure 35 This is a schematic diagram illustrating the structure of the outer tube assembly in a high-throughput pipetting system according to an embodiment of this application.

[0078] Figure 36 This is a schematic diagram of the front door structure in a high-throughput pipetting system according to an embodiment of this application.

[0079] Figure 37 This is a schematic diagram of the pipetting arm in a high-throughput pipetting system according to an embodiment of this application.

[0080] Figure 38 This is a schematic diagram of the Z-axis drive assembly of the pipetting arm in a high-throughput pipetting system according to an embodiment of this application.

[0081] Figure 39 This is a cross-sectional view of the Z-axis drive assembly of the pipetting arm in a high-throughput pipetting system according to an embodiment of this application.

[0082] Figure 40 This is a schematic diagram of the sample plate conveying mechanism of the material transport module in a high-throughput pipetting system according to an embodiment of this application.

[0083] Figure 41 for Figure 40 A cross-sectional view at point QQ.

[0084] Figure 42 This is a schematic diagram of the plate gripping component of the material transfer module in a high-throughput pipetting system according to an embodiment of this application.

[0085] Figure 43 This is a schematic diagram of the reaction plate conveying mechanism of the material transfer module in a high-throughput pipetting system according to an embodiment of this application.

[0086] Figure 44 This is a schematic diagram of the barcode scanning module in a high-throughput pipetting system according to an embodiment of this application.

[0087] Figure label:

[0088] 0001, Sample plate; 0002, Reaction plate;

[0089] 1000, Pipette Module;

[0090] 1100. Pipetting mechanism;

[0091] 1110, Piston chamber assembly; 1111, Piston chamber; 1111a, First quick-change part; 11111, First fixing plate; 11111a, Second receiving groove; 11112, Second fixing plate; 1112, Pipe tip connection part; 11120, Pipe tip; 11121, First receiving groove; 11122, Second sealing ring;

[0092] 1120 Piston shaft assembly; 1121 Piston shaft pressure plate; 1121a Second quick-change part; 1122 Piston shaft; 11221 Rod part; 11222 Fixing part; 1123 Flexible fixing assembly; 11231 Piston shaft fixing plate; 11231a First fixing groove; 11231b Second fixing groove; 11232 Flexible component;

[0093] 1130. Sealing assembly;

[0094] 1200. Suction and spray liquid drive mechanism;

[0095] 1300. Quick-change pipette tip device;

[0096] 1310. Positioning module; 1301. Cavity positioning submodule; 1302. Shaft positioning submodule; 1311. Positioning groove; 13111. Positioning groove; 13112. Positioning inlet; 13113. Clamping port; 13114. First spring seat; 1312. Positioning baffle; 13121. Second spring seat;

[0097] 1320. Quick-change module; 1321. Clamping mechanism; 13211. Clamping block; 13211a. Clamping surface; 1322. Quick-change drive mechanism; 13221. Moving cam block; 13221a. Cam inclined surface; 13222. Linear drive component; 132221. Drive plate; 132222. Locking shaft; 132222a. Drive end; 132222b. Operating part; 13223. Base plate; 132231. Drive slide groove;

[0098] 1330. Reset mechanism; 1331. First elastic reset element; 1332. Second elastic reset element;

[0099] 1400 Head retraction mechanism; 1410 Head retraction plate; 1420 Head retraction drive component; 1421 Head retraction rod; 14211 Protrusion; 1422 Head retraction reset component;

[0100] 1500, Specification identification module; 1510, Identification element; 1520, Sensing element;

[0101] 1600. Interface side panel; 1610. Interface connection board; 1620. Interface mounting plate;

[0102] 1700, First guide mechanism; 1710, First guide bearing; 1720, First guide shaft;

[0103] 2000, Pipette cleaning equipment;

[0104] 2010, Tank structure; 2011, Water inlet chamber; 2011a, Second inclined surface structure; 20111, Single water inlet chamber; 20112, Cross partition; 2012, Drainage chamber; 2012a, First inclined surface structure; 2013, Cleaning tank; 2014, Mounting base plate; 20141, Sealing strip; 2015, Cleaning base;

[0105] 2020, Channel mechanism; 2021, Cleaning channel;

[0106] 2030. Water inlet mechanism; 2031. Water inlet connector;

[0107] 2040, First drainage mechanism;

[0108] 2050, Second drainage mechanism;

[0109] 2060. Overflow monitoring agencies;

[0110] 2070. Ultrasonic cleaning transducer;

[0111] 2080 Automatic water inlet control mechanism; 2081 Liquid storage container; 20811 Water inlet; 20812 Water outlet; 20813 Vent; 2082 Water replenishment component; 20821 Water inlet pipe; 20822 Pressure reducing valve; 20823 Automatic water inlet valve; 2083 Water delivery component; 2084 Liquid level monitoring component;

[0112] 3000, pipette arm;

[0113] 3100, Y-axis drive assembly; 3110, Y-axis displacement unit; 3111, guide rail mounting plate; 3112, first guide rail; 3120, Y-axis drive unit; 3121, Y-axis motor; 3122, Y-axis synchronous pulley; 3123, Y-axis synchronous belt; 3124, synchronous belt connecting plate; 3125, cable connecting plate; 3130, cable chain assembly;

[0114] 3200, Z-axis drive assembly; 3210, Z-axis displacement unit; 3211, Z-axis mounting plate; 3212, second guide shaft; 3212a, guide shaft fixing plate; 3213, second guide bearing; 3214, vertical plate; 3220, Z-axis drive unit; 3221, nut and screw transmission element; 3222, Z-axis synchronous pulley; 3223, Z-axis synchronous belt; 3224, Z-axis motor;

[0115] 4000, Pipette tip support assembly; 4100, Pipette tip base plate; 4200, Support plate; 4300, Pipette tip holder;

[0116] 5000, Frame module; 5100, Machining beam; 5110, Caster parts; 5120, Tabletop; 5200, Crossbeam; 5300, Vertical beam; 5400, Exterior components; 5410, Side panels; 5420, Rear panel; 5430, Top panel; 5440, Front panel; 5441, Front door; 5441a, Constant load spring; 5441b, Lock; 5441c, Breathing light; 5441d, Sub-panel; 5500, Lighting components;

[0117] 6000, Logistics Transmission Module;

[0118] 6100, Sample plate conveying mechanism;

[0119] 6110, Sample plate positioning assembly; 6111, Positioning side plate; 6111a, First coarse positioning ramp; 6112, Elastic side plate; 6112a, Second coarse positioning ramp; 6113, Positioning elastic element; 6113a, Positioning bead; 6113b, Positioning spring;

[0120] 6120. Sample board gripping assembly; 6121. Gripping drive component; 6121a. Gripping base plate; 6121b. Gripping motor; 6121c. Gripping synchronous pulley; 6121d. Gripping synchronous belt; 6121e. Gripping slide rail; 6122. Gripper; 6122a. First gripping plate; 6122b. Second gripping plate;

[0121] 6200, Reaction plate conveying mechanism;

[0122] 6210. Reaction plate positioning assembly; 6211. Reaction plate positioning component;

[0123] 6220. Reaction plate shaping assembly; 6221. Clamping plate; 6222. Clamping drive component;

[0124] 6230, Reaction Plate Conveyor Belt;

[0125] 7000, barcode scanning module; 7100, fixed barcode reader; 7200, mounting bracket; 7300, scanner connector;

[0126] 8000, Stack device;

[0127] 8100, layered stacked modules;

[0128] 8110, Tiered storage unit; 8111, Independent storage space

[0129] 8120, Location Identification Unit;

[0130] 8130, Transport Unit;

[0131] 8131, Lifting assembly; 81311, Lifting motor; 81312, Lifting synchronous pulley; 81313, Lifting synchronous belt; 81313a, Lifting mounting plate; 81314, Lifting guide rail; 81315, Lifting frame; 81316, Counterweight mechanism; 81316a, Pulley structure; 81316b, Wire rope; 81316c, Counterweight block;

[0132] 8132, Rotary assembly; 81321, Rotary motor; 81322, Rotary drive gear; 81323, Rotary driven gear;

[0133] 8133, Telescopic pick-and-place assembly; 81331, Pick-and-place rack; 81332, Pick-and-place tray; 81333, Pick-and-place motor; 81334, Pick-and-place guide rail; 81335, In-situ detection unit;

[0134] 8140, Layered Framework;

[0135] 8200, stacked module;

[0136] 8210, Stacked storage unit; 8211, Stacked cage; 82111, Stacked storage space; 8212, Snap-on mechanism; 82121, Snap-on component; 82122, Snap-on drive assembly; 82122a, Electromagnetic drive component; 82122b, Snap-on reset component; 82122c, Snap-on connecting rod;

[0137] 8220, Transfer Unit;

[0138] 8221. Transfer frame; 82211. Fixed base; 8222. Transfer component; 8223. Transfer drive wheel; 8224. Transfer driven wheel; 8225. Transfer motor;

[0139] 8226. Diffuse reflection sensor;

[0140] 8227, butt joint column;

[0141] 8228, mating hole;

[0142] 8230, Lifting Unit;

[0143] 8231, Lifting tray; 82311, First retaining edge; 82312, Second retaining edge;

[0144] 8232, Lifting drive assembly;

[0145] 8233, Lifting Guide Components;

[0146] 8240, Stacked Frames. Detailed Implementation

[0147] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0148] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0149] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0150] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0151] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0152] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0153] See Figure 1 and Figure 2At least one embodiment of this application proposes a high-throughput pipetting system, which includes a pipetting head module 1000, a pipetting device cleaning device 2000, a pipetting arm 3000, and a stacking device 8000. The pipetting head module 1000 includes a pipetting mechanism 1100, a suction-and-spray mechanism 1200, and a pipetting head quick-change device 1300. The pipetting mechanism 1100 is provided with a pipette tip connection portion 1112. The pipetting mechanism 1100 is detachably connected to the suction-and-spray mechanism 1200 via the pipetting head quick-change device 1300. The suction-and-spray mechanism 1200 drives the pipetting mechanism 1100 to provide positive or negative pressure to the pipette tip connection portion 1112. The pipetting device cleaning device 2000 includes a water inlet mechanism 2030, a first drainage mechanism 2040, and several independent cleaning channels 2021. The water inlet mechanism 2030 is connected to the cleaning channel 2021 to input cleaning fluid, and the first drainage mechanism 2040 is connected to the cleaning channel 2021 to discharge cleaning fluid. Each cleaning channel 2021 is for at least one pipette tip 11120 to extend into. The pipette arm 3000 is drivenly connected to the pipette head module 1000 to drive the pipette head module 1000 to a preset working position. The preset working position includes a sample plate 0001 suction position, a reaction plate 0002 spray position, and a cleaning position. The stacking device 8000 is used to store consumables and to exchange consumables with the pipette head module 1000.

[0154] According to the high-throughput pipetting system of this application embodiment, the pipetting arm 3000 drives the pipetting head module 1000 to move automatically. The pipetting head quick-change device 1300 detachably connects the pipetting mechanism 1100 to the aspiration-spraying liquid drive mechanism 1200, so that the pipetting mechanism 1100 can be separated from the aspiration-spraying liquid drive mechanism 1200. The aspiration-spraying liquid drive mechanism 1200 can connect to pipetting mechanisms 1100 with different channels, such as 96 channels and 384 channels, which can adapt to the pipetting needs of different experimental scenarios such as high throughput and low throughput, without the need to configure a complete set of equipment separately for different throughputs; or pipetting mechanisms 1100 with different volumes, covering various specifications such as 10ul, 30ul, 50ul, 200ul and 1000ul, which can meet the liquid transfer operations of micro-volume, regular volume and even large range, and adapt to the accuracy and range requirements of different experiments; or pipetting mechanisms 1100 with different specifications and different types of consumables from different manufacturers. With the above setup, since the aspiration and spraying liquid drive mechanism 1200 is reusable, only different specifications of pipetting mechanism 1100 need to be replaced to achieve the functions of different specifications of pipettes. There is no need to purchase a complete set of pipetting equipment separately for each pipetting need, which greatly reduces the overall procurement cost of the equipment. At the same time, in the maintenance process, the faulty pipetting mechanism 1100 can be disassembled, repaired or replaced separately without stopping the entire set of equipment for maintenance. Experimenters can quickly replace the corresponding specification of pipetting mechanism 1100 according to real-time experimental needs without re-adjusting the power system of the entire set of equipment. This allows for flexible switching of experimental scenarios, reduces maintenance costs and equipment downtime, and improves experimental efficiency.

[0155] Secondly, the pipetting device cleaning device 2000 in the high-throughput pipetting system of this application embodiment can clean the pipetting tips 11120. During the cleaning process, the water inlet mechanism 2030 delivers cleaning fluid to the cleaning channel 2021. The cleaning fluid cleans the workpiece to be cleaned within the cleaning channel 2021, and the cleaning fluid in the cleaning channel 2021 is discharged through the first drainage mechanism 2040. When the workpiece to be cleaned is configured as a pipetting tip 11120 of the pipetting device, the suction and spraying liquid driving mechanism 1200 drives the pipetting tip 11120 to move, so that the pipetting tip 11120 is inserted into the corresponding independent cleaning channel 2021 one by one. By simulating the repeated suction and discharge of cleaning fluid by the pipetting tip 11120, the cleaning fluid fully contacts the inner and outer walls of the pipetting tip 11120, removing residual liquid, impurities, or contaminants on the pipetting tip 11120, thus achieving the cleaning effect. Since the cleaning channels 2021 corresponding to each pipette tip 11120 are independent, the problem of cross-contamination between pipette tips 11120 can be avoided, ensuring the cleanliness of the cleaning and improving the accuracy of experimental or test results.

[0156] Furthermore, the stacking device 8000 in the high-throughput pipetting system of this application embodiment has a large storage capacity for consumables, reducing the frequency of replenishment and avoiding process interruptions; through automated interactive picking and placing of consumables, it eliminates reliance on manual labor, solves the positioning deviation problem caused by manual operation, improves consumable feeding efficiency and plate making efficiency, and at the same time ensures pipetting accuracy.

[0157] In some embodiments, the stacking device 8000 includes a hierarchical stacking module 8100 and a stacked stacking module 8200. The hierarchical stacking module 8100 includes a plurality of independent storage spaces and is configured to independently access consumables in any of the independent storage spaces. The consumables in the hierarchical stacking module 8100 include a sample plate 0001. The stacked stacking module 8200 includes a stacked storage space 82111 and is configured to sequentially access consumables in the stacked storage space 82111 in a first-in-first-out order. The consumables in the stacked stacking module 8200 include a reaction plate 0002.

[0158] Specifically, the hierarchical stack module 8100's several independent storage spaces are independent of each other and do not interfere with each other, enabling independent access. Consumables can be directly accessed and placed in any independent storage space without following a fixed order, without moving consumables in other spaces. This module is compatible with the stack of sample board 0001. Because the sample information carried within sample board 0001 varies, specific sample board 0001 needs to be flexibly retrieved according to requirements during experiments, without following a fixed order. The independent access capability allows direct location and placement of the target sample board 0001 without moving other consumables.

[0159] The stacked storage space 82111 of the stacked stacking module 8200 is accessed in a first-in, first-out (FIFO) order, meaning consumables are retrieved and placed in the order they are inserted. This is suitable for consumables like reaction plates 0002 that can be processed in batches. Because reaction plates 0002 are produced in high-throughput batches, the large batches of reaction plates are indistinguishable, with identical channel specifications and materials, and no unique sample information. Therefore, the order of retrieval and placement does not affect the experimental results; FIFO retrieval is sufficient without additional precise positioning operations.

[0160] Through the above configuration, the stacking device 8000 uses a combination of layered and stacked stacking to precisely match the characteristics of consumables. For the differentiated information requirements of sample plates 0001, independent access ensures retrieval flexibility and avoids the impact of sample plate 0001 confusion on experimental results. For the undifferentiated characteristics of reaction plates 0002, sequential access simplifies the operation process, eliminating the need for complex positioning mechanisms. Sample plates 0001 do not need to be searched in a fixed order; the target consumable can be retrieved directly, reducing unnecessary actions. Reaction plates 0002 are stacked in batches and retrieved sequentially, increasing storage density while accelerating retrieval speed and reducing process interruptions during high-throughput plate making. The flexible retrieval of sample plates 0001 can quickly respond to different liquid aspiration needs of the pipetting head module 1000, and the sequential retrieval of reaction plates 0002 can match the batch spraying rhythm. Both prevent the transfer and pipetting progress from becoming disconnected, improving the overall plate making efficiency of the system.

[0161] See Figure 3 and Figure 4 In some embodiments, the hierarchical stack module 8100 includes a hierarchical storage unit 8110, a position identification unit, and a handling unit 8130. The hierarchical storage unit 8110 has several layers of independent storage spaces arranged vertically for layered placement of consumables. The position identification unit is used to acquire the identification information and first position information of the consumables, as well as the type information of the hierarchical storage unit 8110. The handling unit 8130 is communicatively connected to the position detection unit and is used to retrieve consumables from the corresponding independent storage space based on the first position information, or to place consumables back into the corresponding independent storage space.

[0162] Specifically, the hierarchical storage unit 8110 divides the vertical space into several independent storage spaces, giving each sample plate 0001 an independent storage location. These independent storage spaces do not interfere with each other, ensuring the independence of sample plate 0001 storage while efficiently utilizing vertical space to increase storage density. The location identification unit collects the identification information of consumables, such as barcodes to distinguish different samples and the first location information corresponding to the consumable, i.e., the coordinates of its independent storage space. It also collects the type information of the hierarchical storage unit 8110, providing support for accurate retrieval and placement. The handling unit 8130 communicates with the location identification unit, receives the first location information transmitted by it, and then accurately locates the independent storage space where the target consumable is located, completing the plate retrieval or placement operation. The entire process does not rely on a fixed order, nor does it require moving consumables in other independent storage spaces. The barcode information includes, but is not limited to, the sample plate 0001 number, sample type, sample plate 0001 type, number of replicated samples, number of replicates, reaction plate 0002 type, reagent information, and reaction plate 0002 number.

[0163] Through the above settings, precise traceability and retrieval of consumables are achieved. The position identification unit binds the identification information of the consumables with the first position information. The stacking device 8000 can automatically find the storage coordinates of the consumables based on the barcode information identified by the position identification unit and automatically complete the output action of the consumables, enabling rapid positioning of any target consumable. The vertically arranged independent storage space maximizes the use of vertical space, increases the number of consumables stored within a limited area, reduces the frequency of replenishment during high-throughput experiments, avoids process interruptions, and improves storage efficiency and space utilization. The handling unit 8130 automatically performs pick-and-place actions according to the first position information without manual intervention, which reduces labor intensity and avoids deviations caused by manual positioning, ensuring the accuracy of docking with the pipetting head module 1000 and improving pipetting accuracy.

[0164] Furthermore, the in-situ detection unit acquires the type information of the hierarchical storage unit 8110, which can adapt to the storage needs of consumables of different specifications. It can meet diverse experimental scenarios without changing modules, thus improving system flexibility. Different types of hierarchical storage units 8110 may have different layer heights and number of layers, but the height of the first layer is the same. The layer height and number of layers parameters of the stacking cage type are stored in the database. The size parameters of the hierarchical storage unit 8110 can be retrieved by scanning the barcode of the hierarchical storage unit 8110.

[0165] In some embodiments, the hierarchical stack module 8100 further includes a hierarchical frame 8140, on which the hierarchical storage unit 8110 and the handling unit 8130 are mounted or connected. To support the stable operation of each unit, the overall hierarchical frame 8140 needs to have a certain rigidity, and the weight of the whole machine is required to be less than or equal to 40kg. Considering manufacturing costs, a riveted frame is formed by bending sheet metal and then riveting it, and an interactive panel is mounted on the riveted frame.

[0166] See Figure 4 In some embodiments, the handling unit 8130 includes a lifting assembly 8131, a rotating assembly 8132, and a telescopic pick-and-place assembly 8133. The lifting assembly 8131 is used to provide lifting driving force; the rotating assembly 8132 is mounted on the lifting assembly 8131 and is used to provide rotational movement about a preset rotation axis; multiple layered storage units 8110 are provided and arranged circumferentially along the preset rotation axis; the telescopic pick-and-place assembly 8133 is mounted on the rotating assembly 8132 and is used to provide telescopic movement in the horizontal direction to pick up and place consumables in independent storage spaces.

[0167] Specifically, the lifting assembly 8131 provides vertical driving force to adapt to the vertical layered structure of the layered storage unit 8110. Based on the layer height information of the target consumable, it drives the entire handling unit 8130 to rise and fall to the corresponding height. The rotating assembly 8132 is installed on the lifting assembly 8131 and provides rotational movement around a preset rotation axis. The layered storage unit 8110 is arranged circumferentially along this rotation axis. By rotating, the rotating assembly 8132 can drive the subsequent telescopic pick-and-place assembly 8133 to align with the independent storage space in different directions, effectively covering all storage positions. The telescopic pick-and-place assembly 8133 is installed on the rotating assembly 8132 and provides telescopic movement in the horizontal direction. When the lifting assembly 8131 is positioned at the target layer height and the rotating assembly 8132 is aligned with the target storage position, the telescopic pick-and-place assembly 8133 extends to pick up and place the consumable in the corresponding independent storage space. After completing the action, it retracts and resets.

[0168] Through the above configuration, the coordinated operation of the lifting component 8131, the rotating component 8132, and the telescopic pick-and-place component 8133 can cover all independent storage spaces of the hierarchical storage unit 8110, meeting the need for flexible retrieval of consumables and avoiding access failures or consumable damage caused by inaccurate positioning. The circumferential arrangement of the hierarchical storage unit 8110, in conjunction with the rotating component 8132, maximizes the storage capacity within a limited footprint, while ensuring smooth pick-and-place operations, reducing unnecessary travel, significantly shortening single access time, and improving the continuity of high-throughput experiments. No manual intervention is required throughout the process; the standardized operation process reduces labor intensity and avoids deviations caused by manual operation. This structure can adapt to storage units with different numbers of layers and different circumferential distributions, and can accommodate various consumable storage requirements without significant modifications. The transport unit 8130 is communicatively connected to the pipetting head module 1000, allowing the movement rhythm of the transport unit 8130 to match the pipetting progress of the pipetting head module 1000, preventing the access action from becoming disconnected from the pipetting process and ensuring the overall plate-making efficiency of the system.

[0169] In some embodiments, the lifting assembly 8131 includes a lifting motor 81311, a lifting synchronous pulley 81312, and a lifting synchronous belt 81313. The lifting motor 81311 is configured as a stepper motor, driving the lifting pulleys to rotate, thereby driving the lifting synchronous belt 81313 to rotate. A lifting mounting plate 81313a is mounted on the lifting synchronous belt 81313, and a rotating assembly 8132 is mounted on the lifting mounting plate 81313a. The lifting assembly 8131 also includes a lifting guide rail 81314, on which the lifting mounting plate 81313a is slidably connected.

[0170] In some embodiments, the lifting assembly 8131 is provided with a counterweight mechanism 81316, which is connected to the load end of the lifting assembly 8131, i.e., the lifting mounting plate 81313a, and is used to balance the load of the lifting assembly 8131. The lifting assembly 8131 needs to drive the rotating assembly 8132, the telescopic picking and placing assembly 8133, and the sample plate 0001 to be picked up and placed to move together. The combined load of these components increases the pressure of the lifting drive. The counterweight mechanism 81316 offsets part of the load with its own weight, making the force on the lifting assembly 8131 more balanced.

[0171] Through the above configuration, the counterweight mechanism 81316 reduces drive energy consumption and losses, avoids lifting and swaying caused by uneven force, ensures the accuracy of longitudinal positioning of the handling unit 8130, and indirectly improves the stability of sample board 0001 picking and placing. At the same time, with the load reduced, the starting, stopping, and speed adjustment response of the lifting component 8131 is more sensitive, shortening the action time. Together with the rotating component 8132 and the telescopic picking and placing component 8133, it improves the overall storage and retrieval efficiency. In addition, the balanced force reduces the deformation risk of the lifting component 8131, avoids structural loosening caused by long-term heavy load, and ensures the stability of the layered stacking module 8100 during long-term high-frequency operation.

[0172] Specifically, the counterweight mechanism 81316 includes a pulley structure 81316a and a wire rope 81316b mounted on the lifting frame 81315. The wire rope 81316b is connected to the pulley structure 81316a. One end of the wire rope 81316b is connected to the counterweight block 81316c, and the other end is connected to the lifting mounting plate 81313a. By changing the direction of force through the pulley structure 81316a, the weight of the counterweight block 81316c is balanced with the load weight of the lifting mounting plate 81313a, thus achieving power-off hovering.

[0173] Through the above configuration, the cooperation between the pulley structure 81316a and the wire rope 81316b ensures that the tension of the counterweight 81316c is evenly transmitted to the lifting mounting plate 81313a, effectively reducing the tension on the synchronous belt and extending the service life of the lifting synchronous belt 81313. The compact structural design does not occupy additional lateral space and is suitable for the compact layout requirements of integrated equipment. The optimized force balance and power-off stability of the lifting component 8131 solve the problems of unreliable suspension and excessive tension in traditional counterweight structures, while also preventing mechanical damage caused by falling during power outages.

[0174] See Figure 5In some embodiments, the rotating assembly 8132 includes a rotary motor 81321, a rotary drive gear 81322, and a rotary driven gear 81323. The rotary motor 81321 is configured as a stepper motor with an encoder and is directly connected to the rotary drive gear 81322. The rotary drive gear 81322 meshes with the rotary driven gear 81323, and the rotary driven gear 81323 is connected to the telescopic pick-and-place assembly 8133.

[0175] See Figure 6 In some embodiments, the telescopic pick-and-place assembly 8133 includes a pick-and-place frame 81331 and a pick-and-place tray 81332, a pick-and-place motor 81333, a pick-and-place synchronous pulley, a pick-and-place synchronous belt, and a pick-and-place guide rail 81334 disposed on the pick-and-place frame 81331. The pick-and-place motor 81333 drives the pick-and-place synchronous pulley to rotate, and the pick-and-place synchronous pulley drives the pick-and-place synchronous belt to rotate. The pick-and-place tray 81332 is fixedly connected to the pick-and-place synchronous belt and slidably connected to the pick-and-place guide rail 81334. Through the above arrangement, the pick-and-place motor 81333 drives the pick-and-place tray 81332 to move telescopically in the horizontal direction.

[0176] In some embodiments, the telescopic take-up and drop-down assembly 8133 is provided with an in-situ detection unit 81335 for detecting whether consumables exist in the independent storage space on the telescopic take-up and drop-down assembly 8133 and at the corresponding position.

[0177] Specifically, the in-situ detection unit 81335 is integrated on the telescopic pick-and-place assembly 8133. On the one hand, it detects whether the telescopic pick-and-place assembly 8133 itself has carried consumables, such as confirming whether the consumables have been successfully picked up after picking up the board, and confirming whether the assembly is empty after placing the board. On the other hand, it detects whether there are consumables in the target independent storage space, such as confirming that there are consumables to be retrieved at this location before picking up the board.

[0178] The above settings prevent accidental operation and damage to consumables, avoid performing a board retrieval operation when there are no consumables in the target space, and avoid performing a board placement operation when there are already consumables in the target space. This also prevents sample board 0001 from being damaged by collision or compression, ensuring the structural safety of consumables and the hierarchical storage unit 8110. The detection results provide a clear signal for starting and stopping the telescopic pick-and-place component 8133, preventing process interruptions due to failed grabbing or misplacement. No manual confirmation of consumable status is required; the entire process of detection, judgment, and action execution is automated, reducing human intervention points, lowering operational complexity, adapting to the continuous operation requirements of high-throughput experiments, and improving the overall board manufacturing process continuity.

[0179] It is understandable that the height of the lifting component 8131 is relatively high, and its perpendicularity to the table surface is greatly affected by assembly and processing errors. In order to reduce the processing difficulty, an adjustment device is designed to make the telescopic picking and placing component 8133 flush with and centered with the target sample plate 0001.

[0180] See Figure 7 In some embodiments, the stackable module 8200 includes a stackable storage unit 8210, a transfer unit 8220, and a lifting unit 8230. The stackable storage unit 8210 is provided with a stackable storage space 82111 for stacking multiple consumables in a vertical direction. The transfer unit 8220 is used to reciprocate horizontally below the stackable storage unit 8210 to feed or remove consumables into or from the stackable storage unit 8210. The lifting unit 8230 is located below the transfer unit 8220 and is used to move vertically to cooperate with the stackable storage unit 8210 to release the bottommost consumable in the stackable storage unit 8210 onto the transfer unit 8220, or to lift the consumables on the transfer unit 8220 and store them in the stackable storage space 82111.

[0181] Specifically, the stacked storage unit 8210 constructs a stacked storage space 82111 along the vertical direction, stacking multiple reaction plates 0002 layer by layer to maximize the use of vertical space and increase the amount of consumables stored per unit area. The transfer unit 8220 is located below the stacked storage unit 8210 and can reciprocate horizontally to remove the reaction plates 0002 from the stacked storage unit 8210 and transfer them to the system interaction position, or to transfer the reaction plates 0002 to be stored to a preset position below the stacked storage unit 8210, thereby realizing the horizontal transfer of consumables. The lifting unit 8230 is located below the transfer unit 8220 and can move up and down vertically. It works in conjunction with the stacking storage unit 8210. When picking up a plate, the lifting unit 8230 rises to receive the reaction plate 0002 at the bottom of the stacking storage unit 8210. After the stacking storage unit 8210 releases the consumables, the lifting unit 8230 descends to place the reaction plate 0002 on the transfer unit 8220. When placing a plate, the lifting unit 8230 rises to lift the reaction plate 0002 on the transfer unit 8220 and send it into the stacking storage space 82111 to complete the storage.

[0182] Through the above settings, vertical stacking increases the number of reaction plates 0002 stored in a single stacking storage unit 8210, reducing the frequency of material replenishment during high-throughput plate making, avoiding process interruptions, and adapting to the batch processing needs of large-scale experiments. Manual loading and unloading of reaction plates is eliminated; the automated picking, placing, and transferring of reaction plates 0002 is achieved through the coordinated action of the stacking module 8200, freeing users from manual labor, reducing labor intensity, and avoiding positioning errors caused by manual operation. The vertical stacking of reaction plates 0002 combined with horizontal transfer ensures standardized and continuous picking and placing operations, with short single-time storage and retrieval times, matching the rhythm requirements of high-throughput plate making and improving overall process efficiency. The horizontal transfer direction of the transfer unit 8220 can interface with the logistics transmission module 6000 or the pipette head module 1000, and the lifting rhythm of the lifting unit 8230 can be matched with the actions of the transfer unit 8220 and the stacking storage unit 8210, ensuring the overall plate making efficiency of the system.

[0183] In some embodiments, the stackable stack module 8200 further includes a stacking frame 8240, and the stacking storage unit 8210, the transfer unit 8220, and the lifting unit 8230 are all mounted or connected to the stacking frame 8240.

[0184] In some embodiments, the stacking storage unit 8210 includes a stacking cage 8211, which is provided with a stacking interface. The stacking interface can be configured as a pin and screw hole. The pin is positioned and engaged with the corresponding position of the transfer unit 8220 to achieve accurate recovery and removal of the reaction plate 0002.

[0185] Specifically, in some embodiments, the limiting structure of the stacking cage 8211 uses eight cylindrical rods, with two cylindrical rods at each of the four corners, corresponding to the four right-angled sides of the reaction plate 0002, forming a surrounding limiting boundary. The cylindrical rods extend vertically, collectively forming the limiting frame of the stacking storage space 82111, ensuring that the reaction plate 0002 is always in a preset position when stacked vertically.

[0186] See Figure 8 In some embodiments, the stacked storage unit 8210 includes a latching mechanism 8212 disposed at the bottom of the stacked storage space 82111. The latching mechanism 8212 includes a rotatable latching member 82121 and a latching drive assembly 82122 drivenly connected to the latching member 82121. The latching member 82121 is used to abut against consumables at the bottom of the stacked storage space 82111 and store them inside the stacked storage space 82111. The latching drive assembly 82122 drives the latching member 82121 to rotate, so as to drive the latching member 82121 to abut against or release consumables.

[0187] Specifically, the latching component 82121 is a rotatable structure used to abut against the bottommost reaction plate 0002 in the stacked storage space 82111. Through mechanical limiting, all stacked reaction plates 0002 are stably stored within the space, preventing consumables from falling out. The latching drive assembly 82122 provides the power for the rotation of the latching component 82121. When it is necessary to store the reaction plate 0002, the latching component 82121 is driven to rotate to the abutment position, cooperating with the lifting unit 8230 to firmly fix the reaction plate 0002 within the stacked space. When it is necessary to release the reaction plate 0002, the latching component 82121 is driven to rotate in the opposite direction to disengage from the abutment, allowing the bottommost reaction plate 0002 to smoothly fall into the transfer unit 8220 with the lifting unit 8230, achieving orderly release.

[0188] With the above configuration, the latching mechanism 8212 is synchronized with the lifting unit 8230 and the transfer unit 8220 through system control, enabling it to complete storage or release without manual intervention, thus meeting the continuous operation requirements of high-throughput plate production. Furthermore, the mechanical latching structure is simple and can accommodate SBS standard reaction plates 0002 of varying thicknesses, satisfying diverse batch storage needs without structural adjustments.

[0189] In some embodiments, the latching drive assembly 82122 includes an electromagnetic drive component 82122a and a latching reset component 82122b. The electromagnetic drive component 82122a is driven to the latching component 82121 and is used to drive the latching component 82121 to rotate when energized. The latching reset component 82122b is driven to the latching component 82121 and is used to provide a reset driving force for the latching component 82121 after the electromagnetic drive component 82122a is de-energized.

[0190] In some embodiments, rotatable latching members 82121 are symmetrically arranged in pairs at the bottom of the stacked storage unit 8210. The front end spacing of their respective parts is designed to be 82.8 mm, which matches the 82.4 mm spacing between the top two ends of the reaction plate 0002 and the 85 mm skirt width. This mechanical limitation enables the stacked reaction plates 0002 to be supported and isolated in layers. The electromagnetic drive 82122a is configured as a telescopic electromagnet, which serves as a power source and is connected to the latching members 82121 via a latching link 82122c. When the bottom reaction plate 0002 needs to be released, the electromagnetic drive 82122a is energized to generate magnetic attraction. The internal magnetic column pulls the latching link 82122c to rotate, thereby causing the rotatable latching members 82121 on both sides to rotate synchronously. This causes the front end of the latching member 82121 to disengage from the skirt of the upper reaction plate 0002, releasing the limitation on the lower reaction plate 0002 and releasing the reaction plate 0002. When reaction plate 0002 descends to the preset distance, the electromagnetic drive component 82122a is de-energized, and the magnetic attraction disappears. The latch reset component 82122b is configured as a torsion spring. The torsion spring releases the pre-stored elastic potential energy, driving the latch linkage 82122c to rotate in the opposite direction, causing the latch component 82121 to spring back and reset. At this time, the front end of the latch component 82121 is re-embedded between the upper and lower reaction plates 0002, and the bottom reaction plate 0002 can fall smoothly to the transfer unit 8220, while the upper reaction plate 0002 is supported by the latch component 82121 and maintains the stacked state.

[0191] With the above settings, the electromagnetic drive responds quickly and can drive the latching component 82121 to rotate rapidly; the latching reset component 82122b ensures that the latching component 82121 is stably reset after power failure, avoiding the latching component 82121 from jamming due to power interruption; the electromagnetic drive structure is simple and small in size, and is suitable for the bottom installation space of the stacked storage unit 8210.

[0192] See Figure 9 In some embodiments, the transfer unit 8220 includes a transfer frame 8221 and a transfer component 8222. The transfer component 8222 carries consumables and extends horizontally, and is movably mounted on the transfer frame 8221 in the horizontal direction. The transfer unit 8220 also includes a transfer motor 8225, a transfer drive wheel 8223, and a transfer driven wheel 8224, which are horizontally spaced apart on the transfer frame 8221. The transfer component 8222 includes a transfer conveyor belt, which is rotatably fitted onto the transfer drive wheel 8223 and the transfer driven wheel 8224. With this design, the transfer conveyor belt is driven to rotate by the transfer drive wheel 8223 and the transfer driven wheel 8224, resulting in a simple structure and high reliability. The transfer conveyor belt can be a flat belt, a V-belt, or other types of belt.

[0193] With the above configuration, the consumables are placed on the transfer member 8222 of the transfer unit 8220. The transfer member 8222 carries the consumables, and by moving the transfer member 8222 horizontally, it can move the consumables horizontally as well. The transfer member 8222 can reciprocate horizontally to move the consumables. In other embodiments, the transfer member 8222 can move only horizontally, as long as it can move the consumables. Furthermore, the transfer member 8222 can move forward or backward in the horizontal direction, depending on the actual usage scenario.

[0194] In some embodiments, the transfer unit 8220 further includes a diffuse reflection sensor 8226 disposed on the transfer frame 8221. When multiple stacked storage units 8210 are arranged horizontally, the transfer frame 8221 is also arranged horizontally with diffuse reflection sensors 8226 at positions corresponding to the stacked storage units 8210 and in corresponding numbers. Each diffuse reflection sensor 8226 is used to detect the presence status of the reaction plate 0002 in the transfer unit to determine whether the plate has been successfully retrieved or delivered.

[0195] In some embodiments, the lifting unit 8230 includes a lifting tray 8231 and a lifting drive assembly 8232. Along the direction in which consumables are fed from the transfer unit 8220 to the stacking storage unit 8210, the lifting tray 8231 is provided with a first stop 82311 and a second stop 82312 in sequence, and the top height of the second stop 82312 is higher than the top height of the first stop 82311. The lifting drive assembly 8232 is drivenly connected to the lifting tray 8231 and is used to drive the lifting tray 8231 to rise and fall to the plate picking station and the positioning station. In the board picking station, the first stop 82311 and the second stop 82312 together abut against the bottommost consumable in the stacked storage unit 8210 to support the consumable; in the positioning station, the second stop 82312 is used to stop the horizontal movement of the consumable on the limiting transfer unit 8220, while the first stop 82311 does not interfere with the horizontal movement of the consumable, so that the consumable is positioned at the preset interactive position for feeding the consumable into the stacked storage unit 8210.

[0196] Specifically, at the board retrieval station, the lifting tray 8231 rises to a height adapted to the bottom of the stacked storage unit 8210. The first and second guard edges 82311 together abut against the reaction plate 0002 at the bottom of the stacked storage unit 8210, forming a stable support. After the latching mechanism 8212 releases the consumable, the lifting tray 8231 drives the reaction plate 0002 to descend smoothly, transferring it to the transfer unit 8220 below. At the positioning station, the lifting tray 8231 rises to a height adapted to the transfer unit 8220. The second guard edge 82312 limits the reaction plate 0002 on the transfer unit 8220 by its own height, preventing it from moving horizontally. At the same time, the first guard edge 82311, being at a lower height, does not interfere with the movement of the consumable, allowing the reaction plate 0002 to be accurately positioned at the preset interactive position in the stacked storage unit 8210, facilitating subsequent lifting and storage.

[0197] With the above settings, at the board retrieval station, the first and second guard edges 82311 and 82312 jointly support the reaction board 0002, preventing the consumable from tilting or falling due to uneven force on a single guard edge. Combined with the release action of the latching mechanism 8212, this ensures the bottom reaction board 0002 is smoothly transferred to the transfer unit 8220. At the positioning station, the second guard edge 82312 precisely stops and limits the movement, while the first guard edge 82311 does not interfere with the transfer, ensuring that the reaction board 0002 is in a preset interactive position before being sent into the stacked storage unit 8210. This avoids storage failure or consumable collision due to positioning deviation, ensuring accurate docking with the stacked storage unit 8210.

[0198] See Figure 11 In some embodiments, the lifting unit 8230 further includes a lifting guide assembly 8233, which includes two vertically arranged lifting guide columns, and the lifting plate is slidably connected to the lifting guide columns.

[0199] In the stacking stack module 8200 of the stacking device 8000 provided in this application embodiment, three stacking stack modes can be set:

[0200] Mode 1: It consists of two stacked storage units 8210 for loading. Both stacked storage units 8210 serve as batch supply units for reaction plates 0002, and output the reaction plates 0002 to be processed to the system in a first-in-first-out order.

[0201] Mode 2: It consists of two stacked storage units 8210 for recycling. Both stacked storage units 8210 serve as batch storage units for reaction plates 0002, storing the processed reaction plates 0002 in a first-in-first-out order.

[0202] Mode 3: It consists of a stacked storage unit 8210 for loading and a stacked storage unit 8210 for recycling. The stacked storage unit 8210 for loading outputs the reaction plate 0002 to be processed, and the stacked storage unit 8210 for recycling simultaneously collects the corresponding processed reaction plate 0002.

[0203] It is understandable that the stack device 8000 can be flexibly configured according to specific usage requirements and is not limited to the three modes mentioned above.

[0204] In some embodiments, the high-throughput pipetting system further includes a material transfer module 6000 for material transfer. The material transfer module 6000 includes a sample plate 0001 conveying mechanism and a reaction plate 0002 conveying mechanism. The sample plate 0001 conveying mechanism interacts with the layered stacking module 8100 to transport the sample plate 0001 to the sample plate 0001 aspiration position; the reaction plate 0002 conveying mechanism interacts with the stacked stacking module 8200 to transport the reaction plate 0002 to the reaction plate 0002 spray position or the next station.

[0205] Specifically, the sample plate 0001 conveying mechanism interacts with the hierarchical stacking module 8100 to receive the independently stored sample plate 0001. When the hierarchical stacking module 8100 retrieves the target sample plate 0001 according to experimental requirements, the sample plate 0001 conveying mechanism interacts with the module to receive the sample plate 0001 and transfer it to the sample plate 0001 aspiration position, providing consumables for the aspiration operation of the pipette head module 1000. Simultaneously, the used sample plate 0001 can be recycled to the corresponding independent storage space of the hierarchical stacking module 8100, or recycled to the stacked storage space 82111 of the stacked stacking module 8200.

[0206] The reaction plate 0002 conveying mechanism interacts with the stacked module 8200 for consumables. After the stacked module 8200 releases the reaction plates 0002 in sequence, the reaction plate 0002 conveying mechanism receives the reaction plates 0002 and transfers them to the liquid spraying position of the reaction plate 0002 for the pipette head module 1000 to complete the liquid spraying operation. After the liquid spraying is completed, the reaction plate 0002 can continue to be transferred to the next station, such as the heat sealing station or the drying station, or recycled to the stacking storage space 82111 of the stacked module 8200.

[0207] Through the above setup, the logistics transmission module 6000 and the stacking device 8000 are used together to replace the manual transfer of consumables, realizing the automated connection of sample plate 0001 and reaction plate 0002 from the stacking device 8000 to the pipetting station. This avoids process interruptions caused by manual handling, solves the problem of low loading efficiency on existing equipment, and adapts to the continuous operation requirements of high-throughput plate production. The conveying mechanism moves along a preset trajectory with high positioning accuracy, accurately delivering sample plate 0001 and reaction plate 0002 to the corresponding liquid aspiration and spraying stations. This avoids the impact of consumable positioning deviations on pipetting accuracy and solves the positioning error problem caused by manual operation.

[0208] See Figure 10 and Figure 11 In some embodiments, the transfer unit 8220 includes a docking structure. Several transfer units 8220 can be arranged horizontally and connected in sequence. Adjacent transfer units 8220 can dock through the docking structure, or they can dock with the logistics transmission module 6000 through the docking structure.

[0209] In some embodiments, the docking structure includes a docking post 8227 and a docking hole 8228 that fit together, with the docking post 8227 and docking hole 8228 respectively disposed on two adjacent transfer units 8220; or one disposed on a transfer unit 8220 and the other disposed on an adjacent logistics transmission module 6000. The above docking structure is simple and easy to operate. Each transfer unit 8220 has a docking post 8227 and a docking hole 8228 at both ends, allowing any transfer unit 8220 to successfully dock during the docking process.

[0210] In other embodiments, other forms of docking structures may also be used. For example, a magnetically coupled docking structure.

[0211] In some embodiments, the transfer unit 8220 further includes a fixing seat 82211. The transfer frame 8221 has fixing seats 82211 at both ends along the horizontal direction. A docking post 8227 and a docking hole 8228 are respectively disposed on the fixing seats 82211 at both ends of the transfer frame 8221. By adjusting the relative positions of the fixing seats 82211 and the transfer frame 8221, the relative positions of the docking structure and the transfer frame 8221 can be adjusted, thereby improving the success rate of alignment between the two transfer units 8220 and increasing alignment efficiency.

[0212] See Figures 15 to 19In some embodiments, the pipetting mechanism 1100 includes a piston cavity assembly 1110 and a piston shaft assembly 1120. The piston cavity assembly includes a plurality of pipette tip connecting portions 1112, and the piston shaft assembly 1120 is slidably connected to the piston cavity assembly 1110. The aspiration and spraying liquid driving mechanism 1200 drives the piston shaft assembly 1120 to reciprocate and slide to provide positive or negative pressure to the pipette tip connecting portions 1112. The piston shaft assembly 1120 includes a piston shaft pressure plate 1121, a piston shaft 1122, and a flexible fixing assembly 1123. The piston shaft pressure plate 1121 is used to connect to the pipetting tip quick-change device 1300 and is detachably connected to the aspiration and spraying liquid driving mechanism 1200 through the pipetting tip quick-change device 1300. The piston shaft 1122 is slidably connected to the piston cavity assembly 1110. The flexible fixing assembly 1123 flexibly connects the piston shaft 1122 to the piston shaft pressure plate 1121, and the piston shaft 1122 reciprocates with the piston shaft pressure plate 1121.

[0213] Specifically, in actual production and assembly processes, slight deviations in the machining accuracy and installation position of the piston shaft pressure plate 1121 and the piston shaft 1122 are inevitable. If a rigid connection is used, these errors will cause the piston shaft 1122's movement trajectory to deviate, and may even lead to problems such as jamming and sealing failure. However, the piston shaft 1122 achieves a flexible connection with the piston shaft pressure plate 1121 through the flexible fixing component 1123. The flexible connection can compensate for these errors through its own deformation or gaps, ensuring that the piston shaft 1122 always slides along the preset trajectory. At the same time, the elimination of assembly errors can ensure the sealing and smooth movement between the piston shaft 1122 and the piston cavity assembly 1110, avoiding air pressure leakage or uneven movement resistance caused by shaft misalignment, thereby stabilizing the liquid volume accuracy of the suction and spraying. The suction and spraying drive mechanism 1200 drives the piston shaft assembly 1120 to reciprocate and slide to provide positive or negative pressure to the suction head connection part 1112, thereby realizing one suction and one spray or one suction and multiple sprays by the pipetting head 11120.

[0214] In some embodiments, the piston shaft 1122 is made of a material with a low coefficient of friction and good wear resistance, such as ceramic or stainless steel. This design reduces friction during sliding of the piston shaft 1122, lowers the power loss of the liquid suction and spraying drive mechanism 1200, ensures drive efficiency, and prevents jamming or trajectory deviation of the piston shaft 1122 due to excessive frictional resistance, thus ensuring smooth liquid suction and spraying operation and accurate liquid transfer. Since the piston shaft 1122 requires long-term, high-frequency reciprocating motion, the wear resistance of the material prevents rapid wear and scratches on its surface, ensuring sealing, preventing leakage, extending the service life of the piston shaft 1122, and reducing equipment maintenance and replacement costs.

[0215] See Figure 19 and Figure 20In some embodiments, the flexible fixing assembly 1123 includes a piston shaft fixing plate 11231 and a flexible member 11232. The piston shaft fixing plate 11231 is fixed below the piston shaft pressure plate 1121. The piston shaft fixing plate 11231 is provided with a first fixing groove 11231a and a second fixing groove 11231b that are connected to each other and pass through the piston shaft fixing plate 11231. The top end of the piston shaft 1122 passes through the first fixing groove 11231a and extends into the second fixing groove 11231b. The flexible member 11232 is disposed in the second fixing groove 11231b and fills the space between the piston shaft 1122 and the piston shaft fixing plate 11231, and / or fills the space between the piston shaft 1122 and the piston shaft pressure plate 1121.

[0216] Combination Figure 17 and Figure 19 In some embodiments, the piston shaft 1122 includes a shaft portion and a fixing portion 11222 disposed at the top end of the shaft portion. The rod portion 11221 is placed in a first fixing groove 11231a, and the fixing portion 11222 is placed in a second fixing groove 11231b. The top end of the fixing portion 11222 abuts against the bottom of the piston shaft pressure plate 1121, and a flexible member 11232 fills the gap between the bottom end of the fixing portion 11222 and the bottom of the groove of the second fixing groove 11231b. The flexible member 11232 fills the gap between the piston shaft pressure plate 1121 and the piston shaft fixing plate 11231, eliminating the backlash difference.

[0217] Specifically, the reciprocating sliding of the shaft enables the pipetting tip 11120 to perform liquid suction and spraying actions; the fixing part 11222 is configured as a protrusion 14211 with a diameter larger than that of the shaft, used for assembly and connection with the piston shaft fixing plate 11231 and the piston shaft pressure plate 1121. The flexible part 11232 itself has compressible deformation characteristics, which can fill the gap between the bottom end of the fixing part 11222 and the bottom of the second fixing groove 11231b, so that the components form a tight contact.

[0218] In some embodiments, the flexible element 11232 includes a first sealing ring, a silicone pad, etc., and is made of a flexible material.

[0219] In some embodiments, the pipette tip connection portion 1112 is disposed at one end of the piston cavity assembly 1110 away from the piston shaft 1122 and communicates with the piston cavity assembly 1110. The pipette tip connection portion 1112 is used for a detachable and sealed connection with the pipette tip 11120. The pipette tip module 1000 also includes a pipette tip retraction mechanism 1400, which includes a pipette tip retraction plate 1410 and a pipette tip retraction drive member 1420. The pipette tip retraction plate 1410 is used to abut against the pipette tip 11120. The pipette tip retraction drive member 1420 is drivenly connected to the pipette tip retraction plate 1410 and is used to drive the pipette tip retraction plate 1410 to abut against the pipette tip 11120 and push the pipette tip 11120 away from the pipette tip connection portion 1112.

[0220] See Figure 17 and Figure 18 The piston cavity assembly 1110 also includes a piston cavity 1111, which is configured as a plurality of cavity structures with independent channels or a multi-channel cavity structure.

[0221] Specifically, the piston chamber 1111 can be configured in two structures to suit different experimental scenarios based on pipetting throughput requirements. When the piston chamber 1111 is configured as a combination of several independent single-channel chambers, each corresponding to a pipetting unit, the channels are independent of each other. If one channel malfunctions or needs replacement, it will not affect the normal operation of other channels. The number of channels can be flexibly combined to meet small to medium throughput pipetting needs, while also facilitating later maintenance and component replacement. When the piston chamber 1111 is configured as a multi-channel chamber structure, its integrated chamber integrates multiple independent channels. This high integration and small space occupation enables high-throughput synchronous pipetting, significantly improving the efficiency of batch pipetting.

[0222] The suction head connection 1112 is disposed on the piston cavity 1111, and the suction head connection 1112 is provided with a suction and spray cavity for the piston shaft 1122 to be inserted. Specifically, when the piston shaft 1122 slides upward, a negative pressure is formed inside the suction and spray cavity, and liquid can be aspirated by the pipette tip 11120; when the piston shaft 1122 slides downward, the pressure inside the suction and spray cavity increases, and liquid is sprayed.

[0223] The bottom of the pipette tip connection 1112 is detachably and sealed to the pipette tip 11120. Specifically, the detachability allows for quick replacement of the pipette tip 11120, meeting the requirements for disposable consumables in experiments and avoiding cross-contamination; the sealing ensures the airtightness of the cavity during the aspiration process, preventing problems such as inaccurate pipetting volume and aspiration failure caused by air pressure leakage.

[0224] See Figure 17In some embodiments, a first receiving groove 11121 is formed on the bottom outer wall of the pipette tip connection 1112, and a second sealing ring 11122 is installed in the first receiving groove 11121. The top opening of the pipette tip 11120 covers the bottom outer wall of the pipette tip connection 1112, and the second sealing ring 11122 achieves a sealing connection between the pipette tip connection 1112 and the pipette tip 11120. When the pipette tip 11120 needs to be replaced, it can be separated from the pipette tip connection 1112 through its top opening under external force, thus achieving disassembly. When the top opening of the pipette tip 11120 covers the bottom outer wall of the pipette tip connection 1112, the inner wall of the pipette tip 11120 will be tightly fitted with the second sealing ring 11122. The elasticity of the second sealing ring 11122 causes it to undergo a certain degree of compression deformation, filling the gap between the outer wall of the pipette tip connection 1112 and the inner wall of the pipette tip 11120, thus forming a reliable sealing layer between them. The first receiving groove 11121 provides installation and limiting space for the second sealing ring 11122, preventing the second sealing ring 11122 from shifting or falling off during the assembly of the pipette tip 11120 or during pipetting, ensuring the stability of the sealing structure. When replacing the pipette tip 11120, only a certain external force needs to be applied to overcome the friction between the second sealing ring 11122 and the inner wall of the pipette tip 11120, causing the top opening of the pipette tip 11120 to separate from the pipette tip connection 1112, completing the disassembly and replacement of the pipette tip 11120. Through the above settings, the requirements for the use of disposable pipette tips 11120 in experiments are met, cross-contamination of samples is avoided, and the reliability of the seal is maintained during pipetting.

[0225] In some embodiments, the bottom outer wall of the pipette tip connector 1112 and the inner wall of the opening of the pipette tip 11120 can be configured for an interference fit to form a seal. Specifically, the inner diameter of the inner wall of the opening of the pipette tip 11120 is slightly smaller than the outer diameter of the bottom outer wall of the pipette tip connector 1112. When the pipette tip 11120 is fitted onto the bottom of the pipette tip connector 1112, a certain amount of external force is required for assembly. After assembly, the inner wall of the pipette tip 11120 will generate a clamping force on the outer wall of the pipette tip connector 1112 due to elastic deformation, so that the two form a tight fit without gaps. With the above configuration, no additional flexible sealing elements such as sealing rings are needed to achieve a seal.

[0226] See Figure 18When the pipetting operation is completed and the pipetting tip 11120 needs to be replaced, the tip retraction drive 1420 outputs power to drive the tip retraction plate 1410 to move towards the pipetting tip 11120. After the tip retraction plate 1410 moves to the preset position, it abuts against the pipetting tip 11120. The tip retraction drive 1420 continues to output thrust, and the tip retraction plate 1410 transmits the force to the pipetting tip 11120. This force overcomes the connection force between the pipetting tip 11120 and the tip connection part 1112. When the force reaches the preset threshold, the pipetting tip 11120 will separate from the bottom outer wall of the tip connection part 1112, completing the disassembly. Afterward, the tip retraction drive 1420 drives the tip retraction plate 1410 to reset, waiting for the next tip retraction.

[0227] In some embodiments, the tip retraction drive 1420 includes a tip retraction rod 1421 and a tip retraction reset member 1422. The tip retraction rod 1421 is slidably disposed in the piston cavity 1111 and fixedly connected to the tip retraction plate 1410. The suction-dispensing liquid drive mechanism 1200 drives the tip retraction rod 1421 to slide through the piston shaft pressure plate 1121, causing the tip retraction plate 1410 to abut against and push the pipette tip 11120. The tip retraction reset member 1422 is connected between the tip retraction rod 1421 and the piston cavity 1111, and / or connected between the tip retraction plate 1410 and the piston cavity 1111. The tip retraction reset member 1422 is used to drive the tip retraction rod 1421 and the tip retraction plate 1410 to reset in a direction away from the pipette tip 11120. This implements a suction-dispensing liquid drive mechanism 1200 as a power source to control the suction-dispensing liquid function and the tip retraction function.

[0228] Specifically, see Figure 18 , Figure 21 and Figure 22The liquid suction and spraying drive mechanism 1200 first drives the piston shaft pressure plate 1121 to reciprocate and slide. Through the flexible fixing component 1123, it drives the piston shaft 1122 to move synchronously. The piston shaft 1122 changes the air pressure in the liquid suction and spraying chamber of the suction head connection part 1112, providing positive or negative pressure to the suction head connection part 1112, thereby completing the liquid spraying and suction actions. During this stage, the head retraction rod 1421 and the head retraction plate 1410 do not participate in the work and are in the standby position. When the aspiration and spraying of liquid is completed and the pipette tip 11120 needs to be disassembled, the aspiration and spraying drive mechanism 1200 continues to drive the piston shaft pressure plate 1121 to move towards the pipette tip 11120. At this time, the piston shaft pressure plate 1121 will touch the pipette tip retraction rod 1421 and drive it to slide synchronously. The pipette tip retraction rod 1421 then pushes the pipette tip retraction plate 1410 to move towards the pipette tip 11120 until the pipette tip retraction plate 1410 abuts against the pipette tip 11120 and applies a pushing force, finally overcoming the connection force between the pipette tip 11120 and the tip connection part 1112, and realizing the disassembly of the pipette tip 11120. After the pipetting head retraction action is completed, the driving force of the suction and spraying mechanism 1200 is removed, and the pipetting head retraction reset member 1422 releases its stored elastic potential energy, generating a pulling force in the direction away from the pipetting head 11120, which drives the pipetting head retraction rod 1421 and the pipetting head retraction plate 1410 back to the initial position, preparing for the next pipetting and retraction cycle.

[0229] With the above settings, there is no need to configure a separate drive source for the head retraction function, which reduces the number of parts in the equipment, simplifies the overall mechanical structure, and reduces production and maintenance costs.

[0230] In some embodiments, the retraction reset member 1422 is configured as a spring. Specifically, the spring is sleeved on the outside of the retraction rod 1421, with one end of the spring abutting against the piston cavity 1111 and the other end connected to the protrusion 14211 of the retraction rod 1421, forming an axial elastic constraint. Alternatively, one end of the spring can be fixed to the bottom of the piston cavity 1111, and the other end connected to the retraction plate 1410.

[0231] Understandably, the head-down drive unit 1420 can also be configured as other power-independent drive units.

[0232] See Figure 17 In some embodiments, the pipetting mechanism 1100 further includes a sealing assembly 1130, which is disposed at the mating connection between the piston cavity assembly 1110 and the piston shaft assembly 1120. The sealing assembly 1130 is used to eliminate mating gaps and ensure the airtightness of the cavity during pipetting.

[0233] Combination Figure 18In some embodiments, the piston cavity assembly 1110 includes a first fixing plate 11111 and a second fixing plate 11112. The first fixing plate 11111 is installed above the piston cavity 1111, and the second fixing plate 11112 has a first through hole for sliding connection of the piston shaft 1122. The second fixing plate 11112 is installed below the piston cavity 1111, and the second fixing plate 11112 has a second through hole for the suction head connection portion 1112 to pass through.

[0234] See Figure 17 and Figure 18 In some embodiments, the sealing assembly 1130 includes a third sealing ring fitted onto the rod portion 11221 of the piston shaft 1122. Specifically, a second receiving groove 11111a is formed on the inner wall of the first through hole, and the third sealing ring is received in the second receiving groove 11111a.

[0235] It is understandable that the sealing assembly 1130 can also be configured as other sealing structures with sealing effect to achieve the sealing of the piston shaft 1122.

[0236] See Figure 14 and Figure 23 In some embodiments, the high-throughput pipetting system further includes a pipette tip support assembly 4000, which supports and positions the pipette tip 11120. Specifically, the pipette tip support assembly 4000 includes a pipette tip base plate 4100, a support plate 4200, and a pipette tip holder 4300. The pipette tip base plate 4100 is fixed to the frame module 5000, and the pipette tip holder 4300 is fixed to the pipette tip base plate 4100 via the support plate 4200. The pipette tip holder 4300 supports the pipette tip 11120. The pipette tip support assembly 4000 is positioned at a preset working position, located between the sample plate 0001 aspiration position and the reaction plate 0002 spray position.

[0237] In some embodiments, the quick-change pipette tip device 1300 can be configured with a bolted connection structure, etc. Other detachable connection structures can be configured according to actual needs, such as snap-fit ​​connections, magnetic connections, pin connections, etc. Different connection structures can adapt to different usage scenarios. For example, snap-fit ​​connections can achieve tool-free quick assembly and disassembly, while magnetic connections can reduce mechanical wear during assembly and disassembly. The overall configuration can be flexibly selected based on factors such as the weight of the pipette mechanism 1100, usage frequency, and accuracy requirements.

[0238] See Figure 24In some embodiments, the quick-change device 1300 for pipetting tips includes a positioning module 1310 and a quick-change module 1320. The positioning module 1310 is used to position the pipetting mechanism 1100. The quick-change module 1320 includes a clamping mechanism 1321 and a quick-change drive mechanism 1322. The clamping mechanism 1321 has a clamping state and a loosening state that clamps or loosens the pipetting mechanism 1100 positioned at the positioning module 1310. The quick-change drive mechanism 1322 is driven to the clamping mechanism 1321 and drives the clamping mechanism 1321 to switch between the clamping state and the loosening state.

[0239] Through the above settings, the positioning module 1310 achieves accurate and stable pre-positioning of the pipetting mechanism 1100, preventing the pipetting mechanism 1100 from shifting or shaking during disassembly, assembly, or operation; the quick-change drive mechanism 1322 of the quick-change module 1320 can drive the clamping mechanism 1321 to flexibly switch between the clamping state and the loosening state. In the clamping state, it can reliably lock the positioned pipetting mechanism 1100, ensuring the structural stability during the pipetting process; in the loosening state, it can quickly release the constraint, and the disassembly of the pipetting mechanism 1100 can be completed without complicated operations. The pre-positioning function of the positioning module 1310 eliminates the need for repeated calibration during the installation of the traditional pipetting mechanism 1100. Operators only need to place the pipetting mechanism 1100 into the positioning module 1310 for initial fixation. The quick-change module 1320 directly controls the state switching of the clamping mechanism 1321 via the quick-change drive mechanism 1322. Locking or loosening the pipetting mechanism 1100 can be achieved simply by operating the quick-change drive mechanism 1322, significantly simplifying the assembly and disassembly process and reducing the time required for a single pipetting mechanism 1100 replacement to a short time, thus solving the problem of cumbersome assembly and disassembly using traditional screw connections. In high-throughput experimental scenarios, it is often necessary to frequently change pipetting mechanisms 1100 of different specifications or those that have already undergone aspiration and dissipation. The modular structure of this device allows for rapid assembly and disassembly of the pipetting mechanism 1100, and the coordinated actions of positioning and quick-change have high repeatability, meeting the operational requirements for high-frequency pipetting mechanism 1100 replacements and preventing experimental interruptions due to excessively long pipetting mechanism 1100 replacement procedures. In summary, by working together with the positioning module 1310 and the quick-change module 1320, the pipetting mechanism 1100 can be quickly locked and disassembled, making the pipetting mechanism 1100 easy to install and disassemble with short time consumption, meeting the need for rapid head changing in high-throughput experimental scenarios, and improving experimental efficiency.

[0240] See Figure 24 and Figure 25In some embodiments, the positioning module 1310 includes a positioning groove 1311, which has a positioning slot 13111, a positioning inlet 13112, and a clamping port 13113 communicating with the positioning slot 13111. The pipetting mechanism 1100 extends into the positioning groove 13111 through the positioning inlet 13112. In the clamping state, the clamping mechanism 1321 extends into the positioning groove 13111 through the clamping port 13113 to apply a clamping force to the pipetting mechanism 1100. The positioning groove 13111, which accommodates the pipetting mechanism 1100, has a shape and size adapted to the part of the pipetting mechanism 1100 that needs to be positioned. The positioning inlet 13112 provides a clear guide path for the insertion of the pipetting mechanism 1100, facilitating quick alignment by the operator.

[0241] With the above setup, the operator pushes the pipetting mechanism 1100 into the positioning groove 13111 through the positioning inlet 13112. The positioning groove 13111 limits the pipetting mechanism 1100, keeping it in a preset installation posture and completing the initial position fixation to prevent the pipetting mechanism 1100 from shifting. When it is necessary to completely fix the pipetting mechanism 1100, the quick-change drive mechanism 1322 of the quick-change module 1320 will drive the clamping mechanism 1321 to move. The clamping mechanism 1321 extends into the positioning groove 13111 through the clamping port 13113, directly acting on the designated part of the pipetting mechanism 1100 and applying clamping force to firmly lock the pipetting mechanism 1100 in the positioning groove 13111, ensuring that the pipetting mechanism 1100 will not loosen during operation.

[0242] In some embodiments, the positioning module 1310 further includes a positioning baffle 1312, which is disposed at one end of the positioning groove 1311 away from the positioning inlet 13112, for the pipetting mechanism 1100 to abut against. The positioning baffle 1312 is a limiting component installed at the end of the positioning groove 1311, away from the positioning inlet 13112, providing a precise stop for the axial travel of the pipetting mechanism 1100, defining the final installation position of the pipetting mechanism 1100 in the positioning groove 13111, and preventing the pipetting mechanism 1100 from deviating in position due to being pushed too deep or too shallow.

[0243] With the above setup, when the operator pushes the pipetting mechanism 1100 into the positioning groove 13111 through the positioning inlet 13112, the pipetting mechanism 1100 will move along the guide direction of the positioning groove 13111 until its end abuts against the positioning baffle 1312. At this point, the pipetting mechanism 1100 reaches the preset standard installation position. No additional calibration is required; the operator can directly determine whether the pipetting mechanism 1100 is properly positioned through the abutment action. The pipetting mechanism 1100 is ultimately constrained to the same position by the positioning baffle 1312, ensuring that pipetting mechanisms 1100 installed by different batches and personnel are in the same working posture, avoiding the impact of installation position differences on subsequent pipetting accuracy. This eliminates the need for manual measurement or observation of whether it is in place; precise positioning can be achieved solely through mechanical abutment, lowering the operational threshold and improving the ease of assembly and disassembly.

[0244] In some embodiments, the clamping mechanism 1321 includes a clamping block 13211, which is slidably connected to the clamping port 13113. The clamping block 13211 is provided with a clamping surface 13211a, which is used to extend into the positioning groove 13111 and abut against the pipetting mechanism 1100. The quick-change drive mechanism 1322 drives the clamping block 13211 to slide back and forth in the clamping port 13113, so as to drive the clamping surface 13211a to abut or separate from the pipetting mechanism 1100. Specifically, the sliding connection between the clamping block 13211 and the clamping port 13113 ensures that the clamping block 13211 can smoothly perform linear reciprocating motion within the clamping port 13113 without jamming. The clamping surface 13211a matches the contour of the part to be clamped in the pipetting mechanism 1100, which increases the contact area with the pipetting mechanism 1100, allowing the clamping force to be applied evenly and avoiding excessive local stress that could damage the pipetting mechanism 1100. At the same time, the clamping port 13113 provides precise motion guidance and stroke constraint for the clamping block 13211, limiting the range of motion of the clamping block 13211.

[0245] In the clamping state, the quick-change drive mechanism 1322 outputs power to push the clamping block 13211 to slide along the clamping opening 13113 towards the positioning groove 13111. At this time, the clamping surface 13211a of the clamping block 13211 extends into the positioning groove 13111 and abuts against the surface of the positioned pipetting mechanism 1100, continuously applying pressure to firmly lock the pipetting mechanism 1100 in the positioning groove 13111, ensuring that the pipetting mechanism 1100 will not loosen or shift during operation. In the releasing state, the quick-change drive mechanism 1322 reverses the drive, causing the clamping block 13211 to slide along the clamping opening 13113 away from the positioning groove 13111. The clamping surface 13211a then separates from the pipetting mechanism 1100, releasing the pressure constraint on the pipetting mechanism 1100. At this time, the operator can easily remove the pipetting mechanism 1100 from the positioning groove 13111, completing the disassembly.

[0246] See Figure 26 In some embodiments, the quick-change drive mechanism 1322 includes a movable cam block 13221 and a linear drive member 13222. The movable cam block 13221 is provided with a cam inclined surface 13221a for abutting against the clamping block 13211. The linear drive member 13222 is drivenly connected to the movable cam block 13221 and can drive the movable cam block 13221 to reciprocate linearly, thereby driving the clamping block 13211 to slide within the clamping port 13113. The quick-change drive mechanism 1322 realizes the conversion of power direction through the transmission of the cam inclined surface 13221a. The overall motion process is divided into two states: clamping drive and releasing drive. Specifically:

[0247] In the clamping state, the linear drive 13222 outputs a positive driving force, pushing the moving cam block 13221 to move linearly. At this time, the cam inclined surface 13221a on the moving cam block 13221 will abut against the contact surface of the clamping block 13211. As the moving cam block 13221 continues to move forward, the cam inclined surface 13221a will generate an upward component force on the clamping block 13211, pushing the clamping block 13211 to slide upward along the clamping port 13113 until the clamping surface 13211a of the clamping block 13211 abuts against the pipetting mechanism 1100 and completes the locking.

[0248] In the released state, the linear drive 13222 outputs a reverse driving force, pulling the moving cam block 13221 to perform a linear reset motion. The contact pressure between the cam inclined surface 13221a and the clamping block 13211 gradually disappears. After the clamping block 13211 loses the support force of the inclined surface, it will slide down along the clamping port 13113, releasing the clamping constraint on the pipetting mechanism 1100 and completing the release action.

[0249] Through the above configuration, the cam inclined surface 13221a converts the horizontal linear motion of the linear drive component 13222 into the vertical lifting motion of the clamping block 13211. This eliminates the need for additional reversing transmission components, resulting in a shorter power transmission path, lower losses, and improved drive efficiency. The tilt angle of the cam inclined surface 13221a is positively correlated with the linear stroke of the moving cam block 13221 and the lifting stroke of the clamping block 13211. By controlling the moving distance of the moving cam block 13221, the clamping stroke and clamping force of the clamping block 13211 can be precisely adjusted. If the quick-change drive mechanism 1322 has multiple clamping blocks 13211, multiple cam inclined surfaces 13221a can be positioned at corresponding locations on one moving cam block 13221 to simultaneously drive all clamping blocks 13211 to lift and lower synchronously, ensuring consistent locking force at each clamping point and improving the stability of the pipetting mechanism 1100.

[0250] In some embodiments, the linear drive 13222 includes a drive plate 132221 and a locking shaft 132222. The drive plate 132221 is mounted on the positioning groove 1311, and the locking shaft 132222 is threadedly connected to the drive plate 132221. The locking shaft 132222 is provided with a drive end 132222a, which is rotatably connected to the movable cam block 13221. The drive plate 132221 provides stable support and motion guidance for the locking shaft 132222, and the drive plate 132221 is machined with an internal threaded hole adapted to the locking shaft 132222 for threaded connection.

[0251] With the above configuration, when the operator rotates the locking shaft 132222, since the locking shaft 132222 and the drive plate 132221 are threadedly connected, the rotational motion of the locking shaft 132222 is converted into a linear reciprocating motion along its own axis. This, in turn, pushes or pulls the movable cam block 13221, which is rotatably connected to it, causing it to reciprocate along a preset path. The movable cam block 13221 then transmits the linear power to the clamping block 13211 through its own cam inclined surface 13221a, completing the clamping or loosening action on the pipetting mechanism 1100. The threaded drive has a fixed lead; for every fixed angle of rotation of the locking shaft 132222, a fixed distance of linear displacement is generated. By controlling the number of rotations of the locking shaft 132222, the stroke of the movable cam block 13221 can be precisely adjusted, thereby controlling the clamping force and stroke of the clamping block 13211, ensuring consistent locking performance. The drive can be completed by manually rotating the locking shaft 132222, without the need for special tools, which improves the convenience of the overall operation.

[0252] In some embodiments, one end of the locking shaft 132222 is provided with an operating part 132222b exposed to the drive plate 132221, which is provided with a knob or anti-slip texture to facilitate operation.

[0253] See Figure 27In some embodiments, the quick-change module 1320 further includes a reset mechanism 1330, which is drivenly connected to the clamping block 13211 and applies a driving force to the clamping block 13211 toward restoring it to the released state. The elastic force of the reset mechanism 1330 does not directly dominate the releasing action of the clamping block 13211, but plays a role in assisting the releasing and resetting with less effort. Specifically, when the quick-change drive mechanism 1322 applies a locking driving force, the elastic reset force generated by the reset mechanism 1330 is completely canceled out by the driving force of the quick-change drive mechanism 1322, and the threaded drive of the quick-change drive mechanism 1322 has a self-locking characteristic, which can firmly lock the clamping position of the clamping block 13211, preventing the reset mechanism 1330 from popping the clamping block 13211 open, thus ensuring the stability of the locking state of the pipetting mechanism 1100. When the operator reverses the quick-change drive mechanism 1322 to release the locking drive force, the reset mechanism 1330 will immediately release the stored elastic force, providing auxiliary power for the reset of the clamping block 13211. The operator only needs to apply a small reverse drive force to drive the clamping block 13211 to quickly return to the loosened state, which greatly reduces the force required for the loosening operation, simplifies the disassembly process of the pipetting mechanism 1100, and strengthens the core function of quick-change of the device.

[0254] In the clamped state, the locking force of the quick-change drive mechanism 1322 is greater than the elastic force of the positioning mechanism, resulting in a force balance. The elastic force of the reset mechanism 1330 exists only as a reserve force and cannot exceed the locking force of the quick-change drive mechanism 1322, thus not affecting the locking stability. In the released state, the locking force of the quick-change drive mechanism 1322 gradually decreases, and the elastic force of the reset mechanism 1330 changes from being canceled out to becoming an auxiliary force, forming a combined force with the operator's reverse operating force to achieve effortless reset. The elastic parameters of the reset mechanism 1330 are precisely calculated to ensure that its elastic force meets the effort-saving requirements for assisted release without exceeding the self-locking critical force of the quick-change drive mechanism 1322.

[0255] With the above settings, in the driving state, the cam inclined surface 13221a of the moving cam block 13221 drives the clamping block 13211 to slide along the clamping port 13113 toward the positioning groove 13111. The reset mechanism 1330 generates elastic deformation and accumulates potential energy as the clamping block 13211 moves. The reset force increases positively correlated with the clamping stroke. Since the locking driving force output by the quick-change drive mechanism 1322 is always greater than the reset force, the two form a force balance, and the clamping block 13211 can stably stay in the clamping position. In the unlocked state, the quick-change drive mechanism 1322 pulls the moving cam block 13221 backward in the opposite direction, and the driving force it applies to the clamping block 13211 is gradually dissipated. The elastic potential energy of the reset mechanism 1330 is then released. The reset force and the operator's reverse operating force form a resultant force, which drives the clamping block 13211 to slide in the opposite direction along the clamping port 13113 to the initial release position, thereby releasing the clamping constraint on the pipetting mechanism 1100.

[0256] Specifically, in some embodiments, the reset mechanism 1330 includes a spring, a torsion spring, an elastic structure, etc.

[0257] In some embodiments, the reset mechanism 1330 includes a first elastic reset member 1331, which is connected between the clamping block 13211 and the positioning groove 1311, and applies a first driving force to the clamping block 13211 to separate it from the pipetting mechanism 1100.

[0258] Specifically, the first elastic reset member 1331 is configured as a first spring, and the positioning groove 1311 is provided with a first spring seat 13114. One end of the first spring is fixedly disposed in the first spring seat 13114, and the other end is connected to the clamping block 13211. When the quick-change drive mechanism 1322 pushes the clamping block 13211 to slide along the clamping port 13113 toward the positioning groove 13111 by the inclined surface of the moving cam block 13221, the first spring will be compressed with the displacement of the clamping block 13211, and the elastic potential energy will be accumulated simultaneously. At this time, the first driving force generated by the first spring will increase with the increase of the clamping stroke, but this force will be completely offset by the locking driving force of the quick-change drive mechanism 1322. Moreover, the threaded self-locking structure of the quick-change drive mechanism 1322 can securely lock the position of the clamping block 13211, so it will not affect the locking stability of the pipetting mechanism 1100. When the operator reverses the operation of the locking shaft 132222, the locking driving force of the quick-change drive mechanism 1322 gradually disappears. The first spring will quickly release the accumulated elastic potential energy and apply a first driving force to the clamping block 13211 in the direction away from the pipetting mechanism 1100. This force, together with the operator's reverse operation force, will cause the clamping block 13211 to reset along the clamping port 13113 until the clamping block 13211 is completely separated from the pipetting mechanism 1100, thus releasing the clamping constraint.

[0259] In some embodiments, the reset mechanism 1330 includes a second elastic reset member 1332, which is drivenly connected to the movable cam block 13221. In the pressed state, the movable cam block 13221 slides to the pressed position, and in the released state, the movable cam block 13221 slides to the released position. The second elastic reset member 1332 applies a second driving force to the movable cam block 13221 to slide from the pressed position to the released position.

[0260] Specifically, the second elastic reset member 1332 is configured as a second spring, and a second spring seat 13121 is provided on the positioning baffle 1312. One end of the first spring is fixedly installed in the second spring seat 13121 of the positioning baffle 1312, and the other end is connected to the moving cam block 13221.

[0261] With the above settings, when the quick-change drive mechanism 1322 drives the movable cam block 13221 to slide towards the pressing position, the second spring will be compressed along with the displacement of the movable cam block 13221, and the elastic potential energy will be accumulated simultaneously. At this time, the second driving force generated by the spring will increase with the increase of the cam block stroke, but this force will be completely offset by the locking driving force of the quick-change drive mechanism 1322. At the same time, the thread self-locking characteristic of the quick-change drive mechanism 1322 can firmly lock the position of the movable cam block 13221. Therefore, the movable cam block 13221 can be stably kept in the pressing position, ensuring the locking effect on the pipetting mechanism 1100. When the operator reverses the locking shaft 132222, the locking driving force of the quick-change drive mechanism 1322 gradually disappears. The second spring will quickly release the accumulated elastic potential energy and apply a second driving force toward the loosened position to the moving cam block 13221. This force, together with the operator's reverse operating force, will drive the moving cam block 13221 to reset to the loosened position. Then, through the transmission of the cam inclined surface 13221a, it will assist the clamping block 13211 to return to its original position, releasing the clamping constraint on the pipetting mechanism 1100.

[0262] In the embodiments of this application, compared with the structure that only sets the first elastic reset member 1331, the second elastic reset member 1332 provides an auxiliary second driving force from the end of the moving cam block 13221, which forms a double assistance with the first driving force at the end of the clamping block 13211, further reducing the force required for the operator to release the operation, greatly shortening the disassembly time of the pipetting mechanism 1100, and enhancing the quick-change function of the device.

[0263] See Figure 27In some embodiments, the quick-change drive mechanism 1322 further includes a base plate 13223, which is disposed at the bottom end of the positioning groove 1311 and abuts against the bottom surface of the moving cam block 13221; the bottom surface of the positioning groove 1311 abuts against the top surface of the moving cam block 13221; wherein, the base plate 13223 and the positioning groove 1311 enclose and construct a drive slide 132231, and the moving cam block 13221 switches between the pressing position and the releasing position in the drive slide 132231 through reciprocating linear motion. Specifically, the base plate 13223 can be configured as a rigid flat plate structure, which is fixed to the bottom of the positioning groove 1311 at a preset installation position by bolts or slots. Its top surface is completely in contact with the bottom surface of the moving cam block 13221. The inner bottom surface of the positioning groove 1311 facing the moving cam block 13221, that is, the lower surface of the groove, is in close contact with the top surface of the moving cam block 13221. The two form a bidirectional limit on the moving cam block 13221, which restricts the displacement of the moving cam block 13221 in the vertical direction and prevents the cam block from moving up and down during the movement.

[0264] With the above settings, when the operator rotates the locking shaft 132222 and the quick-change drive mechanism 1322 outputs a positive driving force, the moving cam block 13221 will slide linearly along the drive slide groove 132231 towards the positioning groove 13111 under the action of the driving force until it reaches the preset pressing position. During this process, the directional constraint of the slide groove can ensure that the movement trajectory of the cam block does not deviate, and the cam inclined surface 13221a on its surface can accurately fit with the driven inclined surface of the pressing block 13211, realizing stable power transmission. When the quick-change drive mechanism 1322 outputs a reverse driving force and the second elastic reset member 1332 provides auxiliary reset power, the moving cam block 13221 will perform a linear reset movement along the drive slide groove 132231 away from the positioning groove 13111 until it returns to the preset release position.

[0265] See Figure 24 and Figure 28 In some embodiments, the positioning module 1310 includes a cavity positioning submodule 1301, which is used to position the piston cavity assembly 1110 of the pipetting mechanism 1100.

[0266] In some embodiments, the positioning module 1310 includes a shaft positioning submodule 1302, which is used to position the piston shaft assembly 1120 of the pipetting mechanism 1100.

[0267] In some embodiments, the pipetting mechanism 1100 includes a suction-dispensing driving mechanism 1200 and a pipetting mechanism 1100. The pipetting mechanism 1100 includes a piston cavity assembly 1110 and a piston shaft assembly 1120. The piston cavity assembly 1110 includes a pipetting tip 11120. The piston shaft assembly 1120 is slidably connected to the piston cavity assembly 1110. The suction-dispensing driving mechanism 1200 is driven to the piston shaft assembly 1120, and drives the piston shaft assembly 1120 to reciprocate and slide to realize the suction and dispensing of liquid by the pipetting tip 11120.

[0268] In some embodiments, the piston shaft assembly 1120 includes a piston shaft pressure plate 1121 and a piston shaft 1122. The piston shaft pressure plate 1121 is used to connect to the pipette head quick-change device 1300 and is detachably connected to the aspiration and spraying liquid drive mechanism 1200 via the pipette head quick-change device 1300. The piston shaft 1122 is slidably connected to the piston cavity assembly 1110.

[0269] In some embodiments, the piston cavity assembly 1110 further includes a piston cavity 1111 and a pipette tip connection portion 1112. The piston cavity 1111 is configured as a plurality of cavity structures with independent channels or a multi-channel cavity structure. The pipette tip connection portion 1112 is disposed on the piston cavity 1111 and is provided with a suction and spraying cavity for the piston shaft 1122 to be inserted. The bottom of the pipette tip connection portion 1112 is detachably and sealingly connected to the pipette tip 11120.

[0270] Specifically, in some embodiments, the piston cavity 1111 of the pipetting mechanism 1100 is provided with first quick-change parts 1111a on both sides. When the cavity positioning submodule 1301 is used to position the piston cavity assembly 1110 of the pipetting mechanism 1100, the positioning groove 1311 of the cavity positioning submodule 1301 is used to cooperate and connect with the first quick-change parts 1111a.

[0271] Specifically, in some embodiments, the piston shaft pressure plate 1121 of the pipetting mechanism 1100 is provided with second quick-change parts 1121a on both sides. When the shaft positioning submodule 1302 is used to position the piston shaft assembly 1120 of the pipetting mechanism 1100, the positioning groove 1311 of the shaft positioning submodule 1302 is used to cooperate and connect with the second quick-change parts 1121a.

[0272] In some embodiments, each positioning groove 13111 in the cavity positioning submodule 1301 is provided with two clamping blocks 13211, and each positioning groove 13111 in the shaft positioning submodule 1302 is provided with one clamping block 13211. The piston cavity assembly 1110 is larger and heavier than the piston shaft assembly 1120. Single-point clamping can easily lead to uneven force on the piston cavity assembly 1110, and may even cause localized stress concentration that damages the piston cavity assembly 1110. Two symmetrically distributed clamping blocks 13211 can simultaneously apply clamping force from both sides of the first quick-change part 1111a, so that the locking force is evenly distributed on both sides of the cavity. This ensures both the firmness of the locking and maintains the horizontal orientation of the cavity, preventing the pipette tip 11120 from tilting due to force on one side, thus affecting the pipetting accuracy. The piston shaft assembly 1120 also needs to reciprocate and move up and down with the first drive component. A single clamping block 13211 can eliminate the axial clearance of the piston shaft 1122. The piston shaft assembly 1120 is lightweight and small in size, and the locking force of single-point clamping can meet the fixing requirements.

[0273] In this embodiment of the application, compared to fixing the piston cavity assembly 1110 only through the quick-change pipette head device 1300, adding the fixing of the piston shaft assembly 1120 can eliminate the gap of the piston shaft 1122 and improve the pipetting accuracy.

[0274] In use, the quick-change pipette tip device 1300 in this embodiment is installed with the pipette mechanism 1100. The piston cavity 1111 of the pipette mechanism 1100 is inserted into the positioning groove 1311 of the cavity positioning submodule 1301, and the piston shaft pressure plate 1121 is inserted into the positioning groove 1311 of the shaft positioning submodule 1302. The pipette mechanism 1100 is pushed to the position where the piston cavity 1111 abuts against the positioning baffle 1312. The locking shaft 132222 is turned by hand. The locking shaft 132222 rotates and moves inward, pushing the moving cam block 13221 inward and pushing the clamping block 13211 upward, locking the piston cavity 1111 and the piston shaft pressure plate 1121, thus fixing the pipette mechanism 1100. Tighten the locking shaft 132222 by hand, and the locking shaft 132222 moves outward, pushing the moving cam block 13221 to move outward. The clamping block 13211 moves downward under the action of the reset mechanism 1330, releasing the piston chamber 1111 and the piston shaft pressure plate 1121, and the pipetting mechanism 1100 is released.

[0275] In some embodiments, the pipetting head module 1000 includes a pair of interface side plates 1620, and the pipetting mechanism 1100 and the quick-change pipetting device are installed between the two interface side plates 1620. Specifically, an interface connection plate 1610 is provided on the top of the interface side plate 1620 for connecting to the pipetting arm 3000.

[0276] A quick-change pipette tip device 1300 is connected to the bottom of the two interface side plates 1620, which is fixedly connected to the cavity positioning submodule 1301. The positioning groove 1311 of the cavity positioning submodule 1301 is used to connect with the first quick-change parts 1111a on both sides of the piston cavity 1111 of the pipetting mechanism 1100. The piston cavity assembly 1110 is detachably connected to the interface side plates 1620 through the quick-change pipette tip device 1300.

[0277] An interface mounting plate 1620 is fixedly connected between the two interface side plates 1620. A suction-and-spray liquid drive mechanism 1200, configured as a lead screw motor, is mounted on the interface mounting plate 1620. The output shaft of the lead screw motor is fixedly connected to the positioning groove 1311 of a quick-change pipette head device 1300, i.e., it is fixedly connected to the shaft positioning submodule 1302. The positioning groove 1311 of the shaft positioning submodule 1302 is used to engage with the second quick-change parts 1121a on both sides of the piston shaft pressure plate 1121 of the pipette mechanism 1100. The piston shaft assembly 1120 is detachably connected to the suction-and-spray liquid drive mechanism 1200 via the quick-change pipette head device 1300.

[0278] With the above configuration, the pipetting head module 1000, through the cavity positioning submodule 1301 and the shaft positioning submodule 1302, respectively positions and locks the piston cavity assembly 1110 and the piston shaft assembly 1120. Compared to the traditional structure that only fixes the cavity, this design eliminates the axial and radial clearances of the piston shaft 1122, ensuring precise control of the piston shaft 1122's stroke and improving the quantitative and positioning accuracy of pipetting.

[0279] In some embodiments, to improve the stability of the shaft positioning submodule 1302 and the piston shaft assembly 1120 during movement, while ensuring the accuracy of the pipette tip 11120 in aspirating and dispensing liquid, the pipette tip module 1000 is further provided with a first guide mechanism 1700. The first guide mechanism 1700 includes a first guide bearing 1710 mounted on the interface mounting plate 1620, and a first guide shaft 1720 connected inside the first guide bearing 1710. The bottom end of the first guide shaft 1720 extends downward and is fixedly connected to the positioning groove 1311 of the shaft positioning submodule 1302. The first guide bearing 1710 and the first guide shaft 1720 provide motion guidance for the shaft positioning submodule 1302 and the piston shaft assembly 1120, further improving the stability of the piston shaft assembly 1120 during movement and ensuring smooth and accurate liquid aspiration and dispensing.

[0280] See Figure 29 and Figure 30In some embodiments, the pipetting device cleaning equipment 2000 includes a tank mechanism 2010, a channel mechanism 2020 disposed within the tank mechanism 2010, a water inlet mechanism 2030 installed on the tank mechanism 2010, and a first drainage mechanism 2040 installed on the tank mechanism 2010.

[0281] See Figure 29 and Figure 30 At least one embodiment of this application provides a pipette cleaning device 2000, which includes a tank mechanism 2010, a channel mechanism 2020, a water inlet mechanism 2030, and a first drainage mechanism 2040. The channel mechanism 2020 is disposed within the tank mechanism 2010 and includes a plurality of independent cleaning channels 2021, each of which allows at least one item to be cleaned to extend into. The water inlet mechanism 2030 is mounted on the tank mechanism 2010 and connected to the cleaning channels 2021 for inputting cleaning fluid into the cleaning channels 2021. The first drainage mechanism 2040 is mounted on the tank mechanism 2010 and connected to the cleaning channels 2021 for discharging the cleaning fluid.

[0282] According to the pipette cleaning device 2000 of this application embodiment, during the cleaning process, the water inlet mechanism 2030 delivers cleaning fluid to the cleaning channel 2021. The cleaning fluid cleans the workpiece to be cleaned within the cleaning channel 2021, and the cleaning fluid in the cleaning channel 2021 is discharged through the first drainage mechanism 2040. When the workpiece to be cleaned is configured as a pipette tip 11120 of the pipette device, the pipette device drives the pipette tip 11120 to move, so that the pipette tip 11120 is inserted into the corresponding independent cleaning channel 2021 one by one. By simulating the repeated aspiration and dispensing of cleaning fluid by the pipette tip 11120, the cleaning fluid fully contacts the inner and outer walls of the pipette tip 11120, removing residual liquid, impurities, or contaminants on the pipette tip 11120, thus achieving the cleaning effect. Since the cleaning channel 2021 corresponding to each pipette tip 11120 is independent of each other, the problem of cross-contamination between pipette tips 11120 can be avoided, ensuring the cleanliness of the cleaning and improving the accuracy of experimental or test results.

[0283] In some embodiments, the cleaning solution is prepared as pure water.

[0284] In some embodiments, the cleaning fluid may also be configured as other cleaning fluids corresponding to cleaning needs.

[0285] In some embodiments, when the pipette tip 11120 of the pipette is configured with 384, the cleaning channel 2021 is correspondingly provided with 384. The cleaning channel 2021 is arranged vertically, and its inner diameter is adapted to the outer diameter of the pipette tip 11120, which facilitates the insertion of the pipette tip 11120 and improves the utilization efficiency of the cleaning solution.

[0286] Understandably, the number and size of the cleaning channels 2021 can be flexibly adjusted according to the specifications of the parts to be cleaned. For example, for pipette tips 11120 with 8 channels, 24 channels, 96 channels, 384 channels, or even 1536 channels, the same number of cleaning channels 2021 can be set up. There is no need to change the main structure of the equipment; only the channel mechanism 2020 needs to be replaced to meet the cleaning requirements of different throughputs, thereby reducing the user's equipment procurement costs.

[0287] See Figure 30 In some embodiments, the tank mechanism 2010 includes a water inlet chamber 2011 and a drain chamber 2012. The water inlet chamber 2011 is connected to the water inlet mechanism 2030. The drain chamber 2012 is independent of the water inlet chamber 2011 and is connected to the first drain mechanism 2040. The cleaning channel 2021 connects the water inlet chamber 2011 and the drain chamber 2012. The cleaning fluid passes sequentially through the water inlet mechanism 2030, the water inlet chamber 2011, the cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040.

[0288] Specifically, the drain chamber 2012 and the inlet chamber 2011 are independent of each other, ensuring that the clean cleaning fluid in the inlet chamber 2011 does not mix with the contaminated cleaning fluid in the drain chamber 2012, thus preventing contamination of the cleaning fluid in the inlet chamber 2011. The inlet mechanism 2030, connected to the inlet chamber 2011, delivers clean cleaning fluid to the inlet chamber 2011; the first drain mechanism 2040, connected to the drain chamber 2012, discharges the contaminated cleaning fluid from the drain chamber 2012. Together, they form a complete closed-loop path for the cleaning fluid through the inlet mechanism 2030, the inlet chamber 2011, the cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040, ensuring a fixed flow direction and no backflow.

[0289] See Figure 31 In some embodiments, the water inlet chamber 2011 includes a plurality of water inlet single chambers 20111, each water inlet single chamber 20111 being connected to the water inlet mechanism 2030; the water inlet mechanism 2030 includes a plurality of water inlet connectors 2031; each water inlet single chamber 20111 is connected to at least two water inlet connectors 2031.

[0290] The single-chamber inlet 20111 enables zoned liquid supply. Compared to the high-flow-rate requirement of a single liquid supply, the zoned liquid supply in this embodiment has a certain buffering and pressure-stabilizing effect. Specifically, the clean cleaning fluid delivered by the inlet mechanism 2030 first enters the single-chamber inlet 20111 of the inlet chamber 2011. The single-chamber inlet 20111 buffers and stabilizes the cleaning fluid, avoiding uneven delivery of the cleaning fluid caused by fluctuations in inlet pressure. The single-chamber inlet 20111 delivers the cleaning fluid more evenly to the corresponding cleaning channel 2021 of each single-chamber inlet 20111, meeting the requirements of high-throughput experiments for uniformity of cleaning effect.

[0291] In addition, if one inlet connector 2031 of a certain inlet single chamber 20111 fails due to pipeline blockage, the other inlet connector 2031 can still maintain most of the liquid supply to the single chamber, avoiding the shutdown of the cleaning channel 2021 corresponding to the entire inlet single chamber 20111 due to the failure of a single connector, thus improving the fault tolerance and reliability of the equipment operation.

[0292] See Figure 31 In some embodiments, four water inlet chambers 20111 are provided, each connected to two water inlet connectors 2031. Specifically, a cross-shaped partition 20112 is provided inside the water inlet chamber 2011; the cross-shaped partition 20112 divides the water inlet chamber 20111 into four independent water inlet chambers 20111 with uniform volume. It can be understood that when the pipetting device has 384 pipetting tips 11120, each water inlet chamber 20111 is connected to 96 cleaning channels 2021, ensuring that the cleaning fluid supply to all cleaning channels 2021 is as consistent as possible.

[0293] With the above settings, the water intake of the water inlet chamber 2011 is distributed in each independent water inlet chamber 20111, thereby improving the uniformity and stability of the water intake.

[0294] Understandably, depending on specific cleaning needs, the water inlet chamber 2011 can be divided into different numbers and / or different volumetric single water inlet chambers 20111 using partitions of different shapes and positions. Each single water inlet chamber 20111 can be connected to a different number of water inlet connectors 2031, such as two, four, or six, and the number and position of the water inlet connectors 2031 are variable.

[0295] In some embodiments, the drain chamber 2012 is located above the water inlet chamber 2011; the cleaning fluid passes sequentially from bottom to top through the water inlet mechanism 2030, the water inlet chamber 2011, the cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040. It is not necessary to consider the flow rate of the cleaning fluid in the cleaning channel 2021; only the drainage flow rate and drainage time of the drain mechanism need to be considered.

[0296] Specifically, when the drain chamber 2012 is located above the inlet chamber 2011, the cleaning fluid needs to overcome gravity and flow upwards under the combined action of the pressure from the inlet mechanism 2030 and the suction pull from the first drain mechanism 2040. The inlet mechanism 2030 delivers clean cleaning fluid to the lower inlet chamber 2011. After a stable pressure is formed in the inlet chamber 2011, the cleaning fluid flows upwards through the cleaning channel 2021 into the upper drain chamber 2012, and is finally discharged by the first drain mechanism 2040. During this process, the cleaning fluid always maintains a unidirectional flow state in the cleaning channel 2021. The contaminated cleaning fluid after use will quickly enter the drain chamber 2012 due to the suction of the first drain mechanism 2040, and will not stagnate in the cleaning channel 2021 or flow back to the inlet chamber 2011 due to gravity, thus completely avoiding the problem of contaminated cleaning fluid mixing with clean cleaning fluid.

[0297] In addition, the upward flow of the cleaning fluid is guided by the combined action of the inlet water pressure and the drainage suction, and the fluid flow state in the cleaning channel 2021 is controlled by the drainage end of the first drainage mechanism 2040. As long as the drainage flow rate of the first drainage mechanism 2040 is large enough and the drainage time is long enough, it can be ensured that there is always fresh cleaning fluid replenishment in the cleaning channel 2021 without the need to frequently adjust the inlet water flow rate.

[0298] See Figure 31 and Figure 32 In some embodiments, the tank mechanism 2010 includes a cleaning tank 2013, a mounting base plate 2014, and a cleaning base 2015. A channel mechanism 2020 is disposed within the cleaning tank 2013, and the upper part of the channel mechanism 2020 and the cleaning tank 2013 enclose a drainage cavity 2012. The mounting base plate 2014 is sealed to the bottom of the cleaning tank 2013, and the lower part of the channel mechanism 2020, together with the cleaning tank 2013 and the mounting base plate 2014, encloses a water inlet cavity 2011. The cleaning tank 2013 and the mounting base plate 2014 are both mounted on the cleaning base 2015.

[0299] In some embodiments, the mounting base plate 2014 is sealed to the cleaning tank 2013 via a sealing strip 20141.

[0300] In some embodiments, the channel mechanism 2020 and the cleaning tank 2013 are connected by a tight fit or adhesive bonding, or the channel mechanism 2020 and the cleaning tank 2013 can be integrally formed or detachably connected.

[0301] See Figure 30 In some embodiments, the pipetting device cleaning equipment 2000 further includes a second drainage mechanism 2050, which is installed on the tank mechanism 2010 and connected to the water inlet chamber 2011, for discharging the cleaning liquid in the water inlet chamber 2011 to prevent cross-contamination.

[0302] Specifically, after the cleaning operation is completed, cleaning fluid may remain on the inner wall of the water inlet chamber 2011 and at the lower end of the cleaning channel 2021. The second drainage mechanism 2050, directly connected to the water inlet chamber 2011, can completely drain the residual cleaning fluid through gravity drainage or low-pressure suction, preventing the residual fluid from remaining in the water inlet chamber 2011 or mixing with the newly injected clean cleaning fluid during the next cleaning. This cuts off the path for residual fluid to contaminate the new cleaning fluid at the source, ensuring that the initial environment for each cleaning is clean and meeting the cleanliness requirements of biological experiments and medical testing.

[0303] See Figure 30 In some embodiments, the bottom of the drain cavity 2012 is inclined towards the first drain mechanism 2040, referred to as the first inclined structure 2012a. The first inclined structure 2012a guides the cleaning fluid in the drain cavity 2012 to be discharged from the first drain mechanism 2040, facilitating drainage. Through the above arrangement, residual cleaning fluid in the drain cavity 2012 is discharged, preventing cleaning fluid contamination. After the contaminated cleaning fluid in the cleaning channel 2021 flows into the drain cavity 2012 under gravity, the inclined structure guides the cleaning fluid to converge towards the interface of the first drain mechanism 2040, preventing the cleaning fluid from accumulating at the bottom of the drain cavity 2012.

[0304] See Figure 30 In some embodiments, the bottom of the water inlet chamber 2011 is inclined toward the second drainage mechanism 2050, referred to as the second inclined structure 2011a. The second inclined structure 2011a guides the cleaning fluid in the water inlet chamber 2011 to be discharged by the second drainage mechanism 2050, facilitating drainage. Through the above arrangement, residual cleaning fluid in the water inlet chamber 2011 is discharged, preventing cleaning fluid contamination.

[0305] It is understandable that the first inclined structure 2012a and the second inclined structure 2011a can be configured to change their inclination direction according to the different positions and numbers of the first drainage mechanism 2040 and the second drainage mechanism 2050. For example, when the first drainage mechanism 2040 is arranged on both sides of the drainage cavity 2012, the first inclined structure 2012a can be inclined from the middle of the drainage cavity 2012 to both sides.

[0306] In some embodiments, the first drainage mechanism 2040 is configured as a first drainage connector; and / or, the second drainage mechanism 2050 is configured as a second drainage connector.

[0307] See Figure 29 and Figure 30In some embodiments, the pipette cleaning device 2000 further includes an overflow monitoring mechanism 2060, which is communicatively connected to the water inlet mechanism 2030 and is used to monitor the liquid level signal of the cleaning fluid in the pipette cleaning device 2000, which is denoted as the first liquid level signal; when the first liquid level signal is abnormal, a stop water inlet signal is sent to the water inlet mechanism 2030.

[0308] When the overflow monitoring agency detects an abnormality in the drainage, it stops the water intake.

[0309] Specifically, the overflow monitoring mechanism 2060 is configured as an overflow detection valve, which has a preset detection line. When the drain chamber 2012 is above the inlet chamber 2011, the overflow detection valve is located on the side wall of the drain chamber 2012. When an abnormal drainage occurs, the cleaning fluid in the drain chamber 2012 will increase. When the first liquid level signal in the drain chamber 2012 reaches the detection line of the overflow monitoring valve, the water intake is stopped to prevent the cleaning fluid from overflowing the tank mechanism 2010.

[0310] Through the above settings, the overflow monitoring mechanism 2060 monitors the first liquid level signal of the cleaning fluid in the tank mechanism 2010 and can respond quickly in case of drainage abnormalities, such as when the first drainage mechanism 2040 is blocked or the drainage rate lags behind the inlet rate. When the liquid level of the cleaning fluid in the drainage chamber 2012 above the inlet chamber 2011 rises to the preset detection line of the overflow detection valve due to poor drainage, the overflow monitoring mechanism 2060 immediately sends a stop inlet signal to the inlet mechanism 2030, cutting off the cleaning fluid supply at the source and preventing the cleaning fluid from accumulating and overflowing into the tank mechanism 2010, thus avoiding pollution of the surrounding environment or damage to other components. The communication connection between the overflow monitoring mechanism 2060 and the inlet mechanism 2030 forms a closed loop of monitoring and control, automatically triggering protective actions without manual intervention. On the one hand, it avoids potential safety hazards such as short circuits and component corrosion caused by overflowing cleaning fluid. On the other hand, timely shutdown of the supply reduces excessive cleaning fluid entering the drain chamber 2012, lowering the pressure on the drain chamber 2012 due to prolonged high liquid levels, extending the structural lifespan of the tank mechanism 2010, and reducing the frequency and cost of equipment maintenance caused by overflow. Furthermore, the overflow monitoring mechanism 2060 replaces traditional manual liquid level checks, providing 24-hour real-time monitoring of the liquid level, especially in high-throughput cleaning scenarios, preventing overflow problems caused by human negligence.

[0311] In some other embodiments, the water inlet chamber 2011 may be located above the drain chamber 2012, and the overflow detection valve may be located on the side wall of the water inlet chamber 2011. This ensures that the flow direction of the cleaning fluid matches its gravity direction, maximizing the flow of the cleaning fluid within the cleaning channel 2021 and improving cleaning cleanliness.

[0312] See Figure 29 and Figure 30 In some embodiments, the pipetting device cleaning equipment 2000 further includes an ultrasonic cleaning transducer 2070, disposed on the tank mechanism 2010, for generating ultrasonic vibrations to the cleaning fluid in the cleaning channel 2021. High-quality cleaning is achieved through the ultrasonic cleaning transducer 2070.

[0313] See Figure 33 In some embodiments, the pipetting device cleaning equipment 2000 further includes an automatic water inlet control mechanism 2080, which includes a liquid storage container 2081, a water replenishment component 2082, a water delivery component 2083, a liquid level monitoring component 2084, and a first controller. The liquid storage container 2081 stores the cleaning solution; the water replenishment component 2082 is connected to the liquid storage container 2081 and replenishes the liquid storage container 2081 with the cleaning solution; the water delivery component 2083 is connected between the liquid storage container 2081 and the water inlet mechanism 2030 and delivers the cleaning solution from the liquid storage container 2081 to the water inlet mechanism 2030; the liquid level monitoring component 2084 is disposed on the liquid storage container 2081 and monitors the liquid level signal of the cleaning solution in the liquid storage container 2081, which is recorded as a second liquid level signal; the first controller is communicatively connected to the liquid level detection mechanism, receives and controls the water replenishment component 2082 and the water delivery component 2083 to start and stop according to the second liquid level signal.

[0314] With the above setup, the automatic water inlet control mechanism 2080 stores cleaning fluid in the storage container 2081, and the liquid level monitoring component 2084 monitors the second liquid level signal in the storage container 2081 in real time. When the liquid level is lower than a preset threshold, the first controller can automatically control the water replenishment component 2082 to start, replenishing the cleaning fluid to the storage container 2081. This eliminates the need for frequent manual checks of the liquid level and manual water addition, making it particularly suitable for high-throughput cleaning scenarios, significantly reducing the frequency of manual intervention and simplifying operations. The water delivery component 2083 connects the storage container 2081 and the water inlet mechanism 2030, continuously supplying cleaning fluid to the water inlet mechanism 2030. Simultaneously, the coordination between the liquid level monitoring component 2084 and the first controller prevents the storage container 2081 from experiencing liquid interruption or overflow.

[0315] In some embodiments, the liquid storage container 2081 is provided with an inlet 20811, an outlet 20812, and a vent 20813. The water replenishment assembly 2082 includes an inlet pipe 20821 and a pressure reducing valve 20822 and an automatic water inlet valve 20823 disposed on the inlet pipe 20821. The pressure reducing valve 20822 stabilizes the pressure of the cleaning fluid input into the liquid storage container 2081. The inlet 20811 is connected to a water source via the inlet pipe 20821. The water delivery assembly 2083 includes an outlet pipe, and the outlet 20812 is connected to the inlet connector 2031 of the water inlet mechanism 2030 via the outlet pipe. The vent 20813 is disposed on the top of the liquid storage container 2081 and is used to communicate with the interior of the liquid storage container 2081 to maintain air pressure balance.

[0316] In some embodiments, the liquid level monitoring component 2084 is configured as a four-float sensor, installed on the top inner wall of the liquid storage container 2081 and extending downward. Specifically, the four-float sensor includes four float contacts arranged in series at intervals along the vertical direction. The four float contacts are located at different heights, and all float contacts are normally open contacts. They are conductive when immersed in the cleaning fluid and disconnected when not immersed in the cleaning fluid. The four float contacts are labeled L1-L4 from top to bottom.

[0317] Specifically, based on the different signal states of the four float contacts, the automatic water inlet control mechanism 2080 operates in the following ways:

[0318] When float contacts L1, L2, L3, and L4 are all in the conducting state, the automatic water intake mode is at the limit level. At this time, it is determined that there is a risk of overflow in the liquid storage container 2081 and an alarm is triggered. The automatic water intake control mechanism 2080 stops working to prevent the continuous replenishment of cleaning fluid from causing overflow.

[0319] When float contact L1 is in the open state and L2, L3, and L4 are in the closed state, the automatic water intake mode is in normal operation. The automatic water intake control mechanism 2080 continues to operate and simultaneously controls the water replenishment component 2082 to stop replenishing cleaning fluid to the liquid storage container 2081, maintaining the current liquid level to meet the water supply requirements.

[0320] When float contacts L1 and L2 are in the open state and L3 and L4 are in the closed state, the automatic water intake mode is in normal operation, and the automatic water intake control mechanism 2080 remains in operation. At this time, the liquid level in the liquid storage container 2081 is in the normal range, and there is no need to adjust the state of the water replenishment component 2082. The cleaning fluid is stably delivered to the water intake mechanism 2030 only through the water delivery component 2083.

[0321] When float contacts L1, L2, and L3 are in the open state and L4 is in the closed state, the automatic water intake mode is in normal operation. The automatic water intake control mechanism 2080 keeps running and simultaneously controls the water replenishment component 2082 to start, replenishing cleaning fluid into the liquid storage container 2081 to avoid the liquid level being too low and affecting subsequent liquid supply.

[0322] When the float contacts L1, L2, L3, and L4 are all in the open state, the automatic water intake mode is in the limited state. At this time, it is determined that there is no cleaning fluid in the liquid storage container 2081 and an alarm is triggered. The automatic water intake control mechanism 2080 stops to prevent the water supply component 2083 from running dry and being damaged.

[0323] When the float contact appears in any combination other than the five states mentioned above, the automatic water intake mode is set to the shutdown level. At this time, it is determined that the second liquid level signal is incorrect, which may be due to sensor failure or abnormal signal transmission. The automatic water intake control mechanism 2080 stops to avoid misoperation caused by signal errors and to ensure the safe operation of the equipment.

[0324] It is understood that the level monitoring component 2084 is not limited to four float sensors; it can be other numbers, more float sensors, or other types of components capable of level monitoring. The number of float sensors can be flexibly adjusted according to actual needs, further enhancing the adaptability of the automatic water inlet control mechanism 2080.

[0325] In some embodiments, the pipetting device cleaning equipment 2000 further includes a second controller, which is communicatively connected to the water inlet mechanism 2030 and the first drainage mechanism 2040 respectively; wherein, the second controller controls the water inlet mechanism 2030 and the first drainage mechanism 2040 to work synchronously, driving the cleaning fluid to form a flow state in the cleaning channel 2021.

[0326] With the above settings, the flowing cleaning fluid can avoid the stagnation of cleaning fluid caused by the traditional method of water inlet followed by water outlet. On the one hand, the contaminated cleaning fluid after use can be discharged in time and will not mix with the newly injected clean cleaning fluid, ensuring that the clean water that comes into contact with the parts to be cleaned is of high purity each time. On the other hand, the flowing cleaning fluid can create a flushing effect on the parts to be cleaned in the cleaning channel 2021. Combined with the repeated suction and discharge action of the pipette tip 11120, it can more efficiently remove residual contaminants from the surface.

[0327] In addition, the synchronous control logic of the second controller can accurately match the inlet flow rate and the outlet flow rate. On the one hand, it prevents the inlet flow rate from exceeding the outlet flow rate, which would cause the liquid level in the outlet chamber 2012 to rise abnormally and avoid triggering the overflow monitoring mechanism 2060 to shut down frequently, thus ensuring the continuity of the cleaning process. On the other hand, it prevents the outlet flow rate from being too large, which would cause the pressure in the inlet chamber 2011 to be too low and prevent insufficient liquid supply to the cleaning channel 2021. Synchronous control can ensure that the cleaning channel 2021 in each independent area can obtain a stable liquid supply, avoid incomplete cleaning of some channels due to pressure imbalance, and ensure the consistency of the cleaning effect of multiple channels.

[0328] See Figure 15 and Figure 16 In some embodiments, the pipetting head module 1000 includes a specification recognition module 1500, which includes a recognition element 1510 and a sensing element 1520. The recognition element 1510 is disposed on the pipetting mechanism 1100, and the sensing element 1520 is disposed on the aspiration and dispensing liquid driving mechanism 1200. The sensing element 1520 recognizes the specification of the pipetting mechanism 1100 through the recognition element 1510.

[0329] The specification recognition module 1500 identifies the specifications of the pipetting mechanism 1100. After the pipetting mechanism 1100 is assembled onto the main body of the device via the pipetting tip quick-change device 1300, the recognition element 1510 and the sensing element 1520 enter the sensing range and form an effective signal connection. The pipetting device can automatically adjust its operating parameters according to the identified specifications of the pipetting mechanism 1100, eliminating the need for manual settings and improving pipetting accuracy and efficiency.

[0330] It is understood that the identification method of the identification element 1510 and the sensing element 1520 can be configured as contact identification and non-contact identification.

[0331] In some embodiments, the identification element 1510 includes a magnet, and the sensing element 1520 includes a Hall sensor. When the magnet is installed on the outer wall of the piston cavity 1111, the Hall sensor is installed on the device body at the position corresponding to the magnet. After the pipetting mechanism 1100 is installed on the device body, the magnet on the outer wall of the piston cavity 1111 enters the sensing area of ​​the Hall sensor. The sensor senses the change in magnetic field strength and then outputs an electrical signal corresponding to the magnetic field. The electronic control system of the device body will pre-store the specification data of the pipetting mechanism 1100 corresponding to different magnet configurations. After receiving the electrical signal from the sensor, the system matches it with the data in the database to automatically determine the specific specifications of the pipetting mechanism 1100 and synchronously adjust the operating parameters of the device.

[0332] Among them, the magnet and the Hall sensor have no mechanical contact, which is a non-contact identification method. It will not cause wear and jamming, can adapt to the high-frequency replacement of the pipetting mechanism 1100, extend the service life of the components, and has high identification stability and reliability.

[0333] In some embodiments, multiple magnets may be provided, which may have different shapes and arrangements in various positions. Different numbers, shapes and arrangements of magnets can be used to distinguish different specifications of pipetting mechanisms 1100.

[0334] In some embodiments, the identification element 1510 includes a micro switch, and the sensing element 1520 includes a mechanical triggering structure. When the micro switch is installed on the outer wall of the piston cavity 1111, the mechanical triggering structure is installed on the device body at the position corresponding to the micro switch. After the pipetting mechanism 1100 is installed on the device body, the mechanical triggering structure on the device body will make precise contact with the micro switch on the outer wall of the piston cavity 1111 and apply a preset mechanical pressure to change the circuit state of the micro switch, such as from open to closed, or from closed to open. The electronic control system of the device body will pre-store the specification data of the pipetting mechanism 1100 corresponding to different micro switch triggering states or triggering combinations. After receiving the on / off signal of the micro switch, the system will match it with the data in the database to automatically determine the specific specifications of the pipetting mechanism 1100 and adjust the operating parameters of the device synchronously.

[0335] Understandably, multiple microswitches can be configured with different shapes or different arrangements to distinguish different specifications of pipetting mechanisms 1100.

[0336] Among them, microswitches and mechanical triggering structures are contact-based identification methods, which provide stable and reliable signals, more accurate identification results, and low cost.

[0337] See Figures 34 to 39 In some embodiments, the high-throughput pipetting system further includes a frame module 5000, with the sample plate 0001 aspiration position, the reaction plate 0002 spray position, and the cleaning position arranged along the Y-axis on the frame module 5000; the pipetting arm 3000 includes a Y-axis drive assembly 3100 and a Z-axis drive assembly 3200 disposed at the output end of the Y-axis drive assembly 3100. The Y-axis drive assembly 3100 is mounted on the frame module 5000 and is used to drive the Z-axis drive assembly 3200 to move along the Y-axis direction; the output end of the Z-axis drive assembly 3200 is drivenly connected to the pipetting head module 1000 and is used to drive the pipetting head module 1000 to move along the Z-axis and Y-axis directions.

[0338] The frame module 5000 provides structural support, ensuring that other modules maintain high precision and stability during operation, reducing vibration and deformation, and protecting key internal components from the influence of the external environment.

[0339] See Figure 34 and Figure 35 In some embodiments, the frame module 5000 includes a frame assembly and an exterior assembly 5400. The frame assembly employs a sheet metal bending and riveting structure and is equipped with machined beams 5100, a tabletop 5120, crossbeams 5200, and vertical beams 5300. Casters 5110 are installed at the bottom of the machined beams 5100. The crossbeams 5200 and vertical beams 5300 employ U-shaped or G-shaped structures to enhance rigidity and strength.

[0340] In some embodiments, the appearance component 5400 is mounted on the frame component to ensure high precision, stability, and protective performance of the equipment operation. The appearance component 5400 includes a top plate 5430, an interface side plate 1620, a rear plate 5420, and a front plate 5440. The front plate 5440 is equipped with a breathing light 5441c and a front door 5441, providing dustproof and safety protection functions. The components of the appearance component 5400 are mainly made of aluminum sheet metal, while the connectors and reinforcing corner pieces are machined from stainless steel. The entire component is mounted on the frame module 5000. The main exterior parts of the appearance component 5400 are powder-coated white, while the front plate 5440 is made of dark acrylic sheet, allowing users to visually observe the equipment's operating process. The front door 5441 is mounted on the front plate 5440 in a sliding manner, meeting the requirements of reasonable height, smooth switching operation, and reliable structure. The breathing light 5441c is configured as an RGB color breathing light strip to indicate the equipment's operating status. Specifically, the front door 5441 is made of black semi-transparent acrylic material. Its double-sided transparent area needs to be processed and protected, and the color separation lines need to be uniform and regular. The internal transparent area needs to be larger than the external transparent area, and both sides and the edges around the door are polished according to the appearance requirements and sprayed with black fine matte umbrella paint.

[0341] See Figure 36In some embodiments, the front door 5441 has a constant load spring 5441a mounted on a roller, and a secondary plate 5441d attached to its end. In use, the constant load spring 5441a, passing through a guide shaft on the roller, serves as one end, while the secondary plate 5441d serves as the other end. Two constant load springs 5441a are fixed to the machined beam 5100 on one side and to the front door 5441 on the other. The weight of the front door 5441 is slightly greater than the load of the constant load springs 5441a. When the operator pushes the front door 5441 upwards, the front door 5441 slides along a preset trajectory. At this time, the constant load spring 5441a is stretched, generating an elastic force opposite to the weight of the front door 5441, offsetting part of the weight of the front door 5441, reducing pushing resistance, and achieving effortless sliding. After the front door 5441 slides to the preset height, it is locked and fixed by the latch 5441b structure, maintaining the open position. The spring remains stretched to balance the weight of the front door 5441 and prevent excessive force on the latch 5441b. After unlocking the latch 5441b, the front door 5441 slowly slides downward to reset under the combined action of its own weight and the elastic restoring force of the constant load spring 5441a, and the spring gradually contracts to its initial state.

[0342] Through the above configuration, the frame assembly provides stable structural support and comprehensive protection, reducing vibration and deformation during equipment operation. It also achieves dust prevention, safety protection, and equipment organization, meeting 5S management goals and resolving the issues of insufficient protection and cluttered appearance of existing equipment. The exterior component 5400 is fixed to the frame assembly with screws, enclosing non-display areas inside the equipment, concealing pipes, wiring, and other messy structures, while preventing dust from entering the equipment, forming a safety barrier.

[0343] See Figure 13 In some embodiments, the frame module 5000 further includes a lighting component 5500 disposed on the frame assembly for providing lighting.

[0344] See Figure 37 , Figure 38 and Figure 39 In some embodiments, the Y-axis drive assembly 3100 of the pipetting arm 3000 includes a Y-axis displacement unit 3110 and a Y-axis drive unit 3120. The Y-axis drive unit 3120 drives the Z-axis drive assembly 3200 to move along the horizontal Y-axis on the Y-axis displacement unit 3110, thereby enabling the pipetting head module 1000 to switch between the sample plate 0001 aspiration position, the reaction plate 0002 spray position, and the cleaning position, ensuring high-throughput pipetting efficiency and positioning accuracy.

[0345] Specifically, the Y-axis displacement unit 3110 includes two guide rail mounting plates 3111. The guide rail mounting plates 3111 serve as guiding references for Y-axis movement and are mounted on the machined beam 5100 of the frame assembly. A first guide rail 3112 is provided on the guide rail mounting plate 3111 along the Y-axis direction, and a first guide rail 3112 connecting plate is slidably connected to the first guide rail 3112. The Y-axis drive unit 3120 includes a synchronous belt drive element, which includes a Y-axis motor 3121, a Y-axis synchronous pulley 3122, a Y-axis synchronous belt 3123, a synchronous belt connecting plate 3124, and a cable connecting plate 3125. The Y-axis motor 3121 is driven by the Y-axis synchronous pulley 3122, which drives the Y-axis synchronous belt 3123 to rotate. The Y-axis synchronous belt 3123 is connected to the first guide rail 3112 connecting plate through the synchronous belt connecting plate 3124. The Z-axis drive assembly 3200 is fixedly connected to the first guide rail 3112 connecting plate.

[0346] The Y-axis drive assembly 3100 also includes a cable chain assembly 3130. The cable chain assembly 3130 includes a cable chain frame mounted on the frame module 5000, and a cable chain connected between the cable chain frame and the guide rail mounting plate 3111. The cable chain assembly 3130 is used to organize and transmit motor cables, preventing cable tangling or wear during movement. A timing belt connecting plate 3124 is screwed to the guide rail mounting plate 3111, and a cable connection plate 3125 is screwed to the timing belt connecting plate 3124. The cable chain connecting plate is mounted to the guide rail mounting plate 3111 with screws, the cable chain frame is fixed to the crossbeam 5200 of the frame module 5000 with screws, and both ends of the cable chain are fixed to the cable chain connecting plate and the cable chain frame with screws respectively.

[0347] With the above configuration, the Y-axis motor 3121 drives the Y-axis synchronous belt 3123 to move through the Y-axis synchronous pulley 3122. The Y-axis synchronous belt 3123 transmits power to the guide rail mounting plate 3111, thereby realizing the smooth horizontal movement of the Z-axis drive assembly 3200 and the pipette module 1000.

[0348] In some embodiments, the Z-axis drive assembly 3200 includes a Z-axis displacement unit 3210 and a Z-axis drive unit 3220. The Z-axis drive unit 3220 can drive the Z-axis to move vertically along the Z-axis, thereby driving the pipetting head module 1000 to move up and down in the vertical direction, realizing vertical displacement of actions such as liquid aspiration, liquid spraying, head retraction, and cleaning.

[0349] Specifically, the Z-axis displacement unit 3210 includes a Z-axis mounting plate 3211 and a second guide mechanism. The Z-axis mounting plate 3211 fixes the Z-axis drive assembly 3200 onto the Y-axis drive assembly 3100, and the second guide mechanism ensures the verticality of the lifting motion. The second guide mechanism includes a second guide shaft 3212 and a second guide bearing 3213, with the second guide bearing 3213 mounted on the Z-axis mounting plate 3211. The second guide shaft 3212 is slidably connected within the second guide bearing 3213 and is connected to the pipetting head module 1000 by a guide shaft fixing plate 3212a.

[0350] The Z-axis drive unit 3220 includes a nut-screw transmission element 3221, a Z-axis synchronous pulley 3222, a Z-axis synchronous belt 3223, a Z-axis motor 3224, and a motor brake. The Z-axis motor 3224 drives the nut to rotate via the Z-axis synchronous belt 3223, thereby driving the screw to rise and fall. The Z-axis motor 3224 is configured as a stepper motor or a servo motor with an encoder. The Z-axis displacement unit 3210 also includes two upright plates 3214, which serve as mounting bases for the Z-axis drive assembly 3200 and connect the Z-axis mounting plate 3211 and the first guide rail 3112 connecting plate. The pipette module 1000 is fixedly connected to the guide shaft fixing plate 3212a of the Z-axis drive assembly 3200 via the pipette quick-change device 1300.

[0351] With the above configuration, the Z-axis motor 3224 outputs rotational power, which is transmitted to the nut via the Z-axis synchronous pulley 3222 and the Z-axis synchronous belt 3223. The rotation of the nut drives the screw to perform linear lifting and lowering motion, realizing the vertical displacement of the pipette head module 1000. The encoder of the motor provides real-time feedback of the position signal, forming a closed-loop control and improving the positioning accuracy of the Z-axis. The Z-axis motor 3224 integrates a brake, which clamps the motor shaft when power is off, realizing Z-axis power-off self-locking and preventing the pipette head module 1000 from falling and damaging the equipment or contaminating the sample. The lifting stroke of the Z-axis drive assembly 3200 is adapted to the vertical height difference of the sample plate 0001, reaction plate 0002, and cleaning tank 2013, ensuring that the pipette head module 1000 can be smoothly inserted into the pipette tip 11120, sample liquid, and cleaning liquid, while reserving space for the tip retraction movement. The nut and screw transmission element 3221 has a compact structure, transmits large torque, adapts to the load requirements of the 384-channel pipette head, and the lifting and lowering movement is smooth and without swaying.

[0352] With the above settings, the pipette arm 3000 features fast movement speed, real-time and accurate position feedback, and high operational precision, which can effectively improve experimental efficiency and accuracy.

[0353] See Figures 40 to 43In some embodiments, the high-throughput pipetting system further includes a material transfer module 6000, which includes a sample plate conveying mechanism 6100 and a reaction plate conveying mechanism 6200. The sample plate conveying mechanism 6100 is used to convey the sample plate 0001 to the sample plate 0001 aspiration position; the reaction plate conveying mechanism 6200 is used to convey the reaction plate 0002 to the reaction plate 0002 spray position or the next station.

[0354] See Figures 40 to 42 In some embodiments, the sample plate conveying mechanism 6100 includes a sample plate gripping assembly 6120 and a sample plate positioning assembly 6110. The sample plate gripping assembly 6120 includes a gripping drive member 6121 and a gripper 6122 drivenly connected to the gripping drive member 6121. The gripping drive member 6121 drives the gripper 6122 to move the sample plate 0001 to the liquid absorption position of the sample plate 0001. The sample plate positioning assembly 6110 includes a positioning side plate 6111 and an elastic side plate 6112. The elastic side plate 6112 is provided with a positioning elastic member 6113 that presses the sample plate 0001 against the positioning side plate 6111 to position the sample plate 0001 at the liquid absorption position of the sample plate 0001.

[0355] Specifically, there are two grippers 6122. Each gripper 6122 is equipped with a first gripping plate 6122a and a second gripping plate 6122b. The first gripping plate 6122a is L-shaped and is used to position one corner of the sample plate 0001. The second gripping plate 6122b is used to abut and position the sample plate 0001 at the edge away from the first gripper 6122. The first gripping plate 6122a and the second gripping plate 6122b of the two grippers 6122 together enclose and construct a gripping space. The sample plate 0001 is placed in the gripping space and can move with the grippers 6122.

[0356] In some embodiments, the gripping drive 6121 includes a gripping base plate 6121a, a gripping motor 6121b, a gripping timing pulley 6121c, a gripping timing belt 6121d, and a gripping slide rail 6121e. The gripping base plate 6121a is mounted on the frame module 5000. Both the gripping motor 6121b and the gripping slide rail 6121e are mounted on the gripping base plate 6121a. The output shaft of the gripping motor 6121b is driven by the gripping timing pulley 6121c, which drives the gripping timing belt 6121d to rotate. The gripper 6122 is fixedly connected to the gripping timing belt 6121d and slidably connected to the gripping slide rail 6121e.

[0357] With the above configuration, the gripping motor 6121b drives the gripping synchronous wheel 6121c to rotate, and the gripping synchronous wheel 6121c drives the gripper 6122 to move the sample plate 0001 to the liquid absorption position of the sample plate 0001 through the gripping synchronous belt 6121d.

[0358] In some embodiments, the ends of the positioning side plate 6111 and the elastic side plate 6112 of the sample plate positioning assembly 6110 are respectively provided with a first coarse positioning slope 6111a and a second coarse positioning slope 6112a. The first coarse positioning slope 6111a and the second coarse positioning slope 6112a form an open flared structure for providing coarse positioning guidance for the sample plate 0001 to enter between the positioning side plate 6111 and the elastic side plate 6112. The inner wall of the elastic side plate 6112 is provided with a positioning elastic element 6113, which includes a positioning bead 6113a and a positioning spring 6113b connected between the positioning bead 6113a and the elastic side plate 6112. Multiple positioning elastic elements 6113 may be provided. Under the elastic force of the positioning spring 6113b, the positioning bead 6113a is pressed against the side wall of the sample plate 0001, thereby pressing the sample plate 0001 against the positioning side plate 6111.

[0359] With the above settings, the sample plate positioning component 6110 can be adapted to sample plates 0001 with different sizes to achieve accurate positioning.

[0360] It is understood that in other embodiments, the sample plate positioning component 6110 may also be configured as a central positioning structure. Specifically, the sample plate positioning component 6110 includes two positioning side plates 6111, and the inner walls of the two positioning side plates 6111 are provided with positioning elastic members 6113. The positioning elastic members 6113 on both sides can abut the sample plate 0001 placed in the two positioning side plates 6111 at the central position.

[0361] In this embodiment, the positioning elastic element 6113 can be configured as a combination of positioning bead 6113a and positioning spring 6113b, or it can be directly configured as a flexible spring or a spring sheet structure, etc., which will not be elaborated here.

[0362] See Figure 43 In some embodiments, the reaction plate conveying mechanism 6200 includes a reaction plate positioning assembly 6210 and a reaction plate shaping assembly 6220. The reaction plate positioning assembly 6210 includes a reaction plate positioning member 6211, which is used to position the reaction plate 0002 at the liquid spraying position of the reaction plate 0002. The reaction plate shaping assembly 6220 includes a pressing plate 6221 and a pressing drive member 6222 driven by the pressing plate 6221. The pressing drive member 6222 drives the pressing plate 6221 to press against the upper surface of the reaction plate 0002 to shape its flatness. The reaction plate conveying mechanism 6200 also includes two reaction plate conveyor belts 6230 for conveying the reaction plate 0002.

[0363] In some embodiments, the reaction plate positioning member 6211 is configured as a reaction plate clamping arm. When the reaction plate conveying mechanism 6200 drives the reaction plate 0002 to the liquid spraying position, the reaction plate clamping arm rotates to clamp the reaction plate 0002. Two sets of pressure plates 6221 are provided, respectively placed on both sides above the pressure plates 6221. The pressure drive motor drives the pressure plates 6221 to press against the upper surface of the reaction plate 0002, shaping the flatness of the reaction plate 0002. After the plate making is completed, the reaction plate clamping arm rotates to reset, the pressure plates 6221 are lifted to reset, and the reaction plate 0002 can be conveyed to the next station by the reaction plate conveying mechanism 6200.

[0364] It is understandable that the reaction plate positioning component 6211 can also be configured as a positioning structure for other linear motion positioning components, capable of limiting and positioning the reaction plate 0002 at the liquid spray position. The shaping position and number of the clamping plate 6221 are variable. Its shaping position can be at the four corners of the reaction plate 0002 or at the middle position of the four edges of the reaction plate 0002; the number of clamping plates 6221 can be four or six. Alternatively, the clamping plate 6221 can be installed directly on the pipetting head module 1000 without a separate clamping drive component 6222, sharing the drive of the pipetting arm 3000 with the pipetting head module 1000.

[0365] See Figure 44 In some embodiments, the high-throughput pipetting system further includes a barcode scanning module 7000, which is located upstream of the spray position of the reaction plate 0002 and is used to record barcode information on the reaction plate 0002 before spraying.

[0366] In some embodiments, the barcode scanning module 7000 is mounted on the frame module 5000. Specifically, the barcode scanning module 7000 includes a fixed barcode reader 7100, a mounting bracket 7200, and a scanner connector 7300. The mounting bracket 7200 is mounted on the frame module 5000, and the fixed barcode reader 7100 is connected to the mounting bracket 7200 via the scanner connector 7300. The scanner connector 7300 is rotatably connected to the mounting bracket 7200 to adjust the scanning angle of the fixed barcode reader 7100. This allows for flexible adjustment of the scanning angle to accommodate differences in barcode position on reaction plates 0002 of different specifications, as well as slight placement deviations of the reaction plates 0002 within the logistics transmission module 6000. This eliminates the need for frequent adjustments to the device or the placement of the reaction plates 0002, reducing operational complexity and improving scanning accuracy.

[0367] With the above setup, the barcode scanning module 7000 records the barcode information of reaction plate 0002 before spraying, binding the sample source, plate preparation parameters, and other data of each reaction plate 0002 to the barcode. Subsequently, the corresponding experimental data can be quickly retrieved via the barcode, effectively avoiding confusion of reaction plates 0002 in multiple batches of experiments. This meets the traceability requirements of biological experiments and medical testing, improving the reliability of experimental results. The barcode scanning module 7000 is located upstream of the spraying position of reaction plate 0002, completing the scanning process during the transport of reaction plate 0002 to the spraying position. This eliminates the need for an additional independent scanning step, does not occupy plate preparation time, ensures high-throughput plate preparation efficiency, and improves the automation level of the high-throughput pipetting plate preparation system.

[0368] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0369] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A high-throughput pipetting and plating system, characterized in that, The application relates to a pipetting device and a stack device thereof. The pipetting device comprises a pipetting head module, a pipetting device cleaning device and a pipetting arm. The pipetting head module comprises a pipetting mechanism, a suction and spraying liquid driving mechanism and a pipetting head quick-change device. The pipetting mechanism is provided with a suction head connecting portion. The pipetting mechanism is detachably connected with the suction and spraying liquid driving mechanism through the pipetting head quick-change device.

2. The high throughput pipetting and platting system of claim 1, wherein, The suction and spraying liquid driving mechanism drives the pipetting mechanism to provide positive pressure or negative pressure to the suction head connecting portion. The pipetting device cleaning device comprises a water inlet mechanism, a first water outlet mechanism and a plurality of independent cleaning channels. The water inlet mechanism is connected with the cleaning channels to input cleaning liquid.

3. The high throughput pipetting and platting system of claim 2, wherein, The first water outlet mechanism is connected with the cleaning channels to discharge the cleaning liquid. Each cleaning channel is provided with at least one pipetting suction head. The pipetting arm is drivingly connected with the pipetting head module and is used for driving the pipetting head module to move to a preset working position. The preset working position comprises a sample plate liquid suction position, a reaction plate liquid spraying position and a cleaning position.

4. The high throughput pipetting and platting system of claim 3, wherein, The stack device is used for storing consumables and performing consumable interaction between the consumables and the pipetting head module. The stack device comprises a layered stack module and a stack stack module. The layered stack module comprises a plurality of independent storage spaces and is configured to independently access the consumables in any independent storage space. The consumables comprise sample plates.

5. The high throughput pipetting and platting system of claim 4, wherein, The stack stack module comprises a stack storage space and is configured to sequentially access the consumables in the stack storage space in a first-in-first-out order. The consumables comprise reaction plates.

6. The high throughput pipetting and platting system of claim 2, wherein, The layered stack module comprises a layered storage unit and a position recognition unit. The layered storage unit is provided with a plurality of layers of independent storage spaces arranged in a vertical direction and is used for layering the consumables. The position recognition unit is used for acquiring identification information and first position information of the consumables and acquiring type information of the layered storage unit. A carrying unit is communicatively connected with the position detection unit and is used for taking out the consumables in the independent storage space at a corresponding position according to the first position information or placing the consumables back into the independent storage space at the corresponding position. The carrying unit comprises a lifting assembly and a rotating assembly. The lifting assembly is used for providing lifting driving force. The rotating assembly is mounted on the lifting assembly and is used for providing rotation movement around a preset rotation axis. The layered storage unit is provided with a plurality of layers and is arranged in a circumferential direction of the preset rotation axis. A telescopic taking and placing assembly is mounted on the rotating assembly and is used for providing telescopic movement in a horizontal direction to take and place the consumables in the independent storage space. The lifting assembly is provided with a counterweight mechanism connected to a load end of the lifting assembly and is used for balancing the load of the lifting assembly. And / or, the telescopic taking and placing assembly is provided with an in-place detection unit used for detecting whether the consumables exist in the independent storage space at a corresponding position on the telescopic taking and placing assembly. The stack stack module comprises a stack storage unit and a transfer unit. The stack storage unit is used for stacking a plurality of consumables in a vertical direction. The transfer unit is used for reciprocating movement in a horizontal direction below the stack storage unit to send the consumables into or take the consumables out of the stack storage unit. A lifting unit is arranged below the transfer unit and is configured to move in a vertical direction to cooperate with the stack storage unit to release the consumable at the bottom layer of the stack storage unit to the transfer unit or to lift and store the consumable on the transfer unit into the stack storage space.

7. The high throughput pipetting and platting system of claim 6, wherein, The stack storage unit comprises: A buckle mechanism is arranged at the bottom of the stack storage space. The buckle mechanism comprises a rotatable buckle member and a buckle driving assembly drivingly connected with the buckle member. The buckle member is configured to abut against the consumable at the bottom of the stack storage space and store the consumable into the stack storage space. The buckle driving assembly drives the buckle member to rotate to drive the buckle member to abut against and store or release the consumable.

8. The high throughput pipetting and platting system of claim 7, wherein, The buckle driving assembly comprises: An electromagnetic driving member drivingly connected with the buckle member and configured to drive the buckle member to rotate when powered; A buckle reset member drivingly connected with the buckle member and configured to provide a reset driving force for the buckle member after the electromagnetic driving member is powered off.

9. The high throughput pipetting and platting system of claim 7, wherein, The lifting unit comprises: A lifting tray arranged in a direction in which the transfer unit feeds the consumable to the stack storage unit. The lifting tray is sequentially provided with a first stop edge and a second stop edge. The top height of the second stop edge is higher than that of the first stop edge. A lifting driving assembly drivingly connected with the lifting tray and configured to drive the lifting tray to ascend to a plate taking station and a positioning station. In the plate taking station, the first stop edge and the second stop edge jointly abut against the consumable at the bottom layer of the stack storage unit to support the consumable. In the positioning station, the second stop edge is configured to stop and limit the horizontal movement of the consumable on the transfer unit, while the first stop edge does not interfere with the horizontal movement of the consumable, so that the consumable is positioned at a preset interaction position for feeding the consumable to the stack storage unit.

10. The high throughput pipetting and platting system of claim 2, wherein, The high-throughput pipetting plate making system further comprises a logistics transmission module configured to transmit the consumable. The logistics transmission module comprises: A sample plate conveying mechanism configured to interact with the layered stack module to convey the sample plate to a sample plate liquid suction position. A reaction plate conveying mechanism configured to interact with the stack stack module to convey the reaction plate to a reaction plate liquid injection position or a next station.

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