Method for rapidly identifying activity of plum-leaf crab seeds based on conductivity-paper roll method

Abstract

The invention discloses a conductivity-paper roll method-based method for rapidly identifying the vigor of plum-leaf crab seeds, and relates to the technical field of plant cultivation, and the method comprises the following steps: carrying out seed sampling, cleaning and moisture content balance treatment; measuring the conductivity of the seed leachate and judging the integrity of a cell membrane; culturing germination by adopting a wet paper roll method, and calculating a comprehensive vitality index; and comprehensively judging a batch release result according to a threshold value and a rechecking mechanism. According to the method, the cell membrane permeability is represented through the conductivity, the germination potential is reflected through the germination rate, a quantitative threshold value is established by combining double indexes, rapid grading of the seed vigor is achieved, compared with a traditional germination method, the detection period is shortened from 28 days to 14 days or less, operation is easy and convenient, chemical dyes are not needed, the result is traceable, repeatability is high, and the method is suitable for large-scale popularization and application. The method can be widely applied to activity detection, batch release and quality control of the plum-leaf crab and other fruit tree seeds.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cultivation, in particular to a Malus micromalus Chun seed vigor rapid identification method based on conductivity-paper roll method. BACKGROUND

[0002] The Malus micromalus Chun seed vigor level is a key indicator affecting seedling propagation quality and survival rate. Existing seed vigor detection mainly uses conventional germination test method or TTC (2,3,5-triphenyltetrazolium chloride) staining method. Among them, the conventional germination test has a long cycle, usually more than 28 days to obtain stable results, which is difficult to meet the needs of seed batch release and rapid classification; while the TTC staining method has a shorter detection time, but needs to use chemical dyes and microscopic analysis, which is complicated, high in cost and has human error in interpretation.

[0003] In addition, the existing detection methods are based on end-point results, lack of quantitative evaluation of cell membrane integrity and early germination potential, and cannot realize rapid and objective determination of seed vigor. Therefore, we propose a Malus micromalus Chun seed vigor rapid identification method based on conductivity-paper roll method. SUMMARY

[0004] The purpose of the present application is to provide a Malus micromalus Chun seed vigor rapid identification method based on conductivity-paper roll method to solve the problems raised in the background.

[0005] To achieve the above purpose, the present application provides the following technical scheme: a Malus micromalus Chun seed vigor rapid identification method based on conductivity-paper roll method, comprising the following steps:

[0006] Step 1, sampling, washing and water content balancing of Malus micromalus Chun seeds;

[0007] Step 2, determination of seed leachate conductivity and judgment of cell membrane integrity;

[0008] Step 3, wet paper roll method for germination culture and calculation of comprehensive vigor index;

[0009] Step 4, batch release result judgment according to threshold value and review mechanism.

[0010] Preferably, the step 1 comprises:

[0011] Randomly take 100 seeds, rinse with deionized water for 30 seconds and dry naturally;

[0012] Detect the initial water content of the seeds, and when the water content is > 12%, dry at 25℃ for 2h to eliminate the difference in water content;

[0013] Number the samples and establish a batch database for subsequent result tracing.

[0014] Preferably, the step 2 comprises:

[0015] The seeds are soaked in 25℃ deionized water for 24h and then taken out;

[0016] The conductivity of the leaching solution is measured by a conductivity meter with an electrode constant of 1.0, and calibrated with 1413μS·cm -1 ;

[0017] The specific conductivity E is calculated, and if E≤40μS·cm -1 ·g -1 , the cell membrane is determined to be intact, otherwise, the abnormal review process is entered.

[0018] Preferably, the abnormal review process comprises:

[0019] The conductivity is repeatedly measured and the deviation is calculated ;

[0020] If ≤3μS·cm -1 ·g -1 , it is determined to be measurement error and the average value is taken;

[0021] If >3μS·cm -1 ·g -1 , it is marked as a membrane damage sample.

[0022] Preferably, the step 3 comprises:

[0023] A wet paper roll is prepared using quantitative filter paper of 120g·m -2 and qualitative filter paper with a moisture content of 65%-70%;

[0024] The treated seeds are cultured in a dark environment at 20℃±0.5℃ and a relative humidity of 90%;

[0025] The germination potential F7 is counted at 7d, and the germination rate F 14 is counted at 14d, and if F7≥60% and F 14 ≥85%, the batch is considered to be preliminarily qualified.

[0026] Preferably, the step 4 comprises:

[0027] The vitality index formula is established:

[0028] ;

[0029] Wherein, E is the measured conductivity, and E0 is the upper limit threshold of conductivity, 40μS·cm -1 ·g -1 ;

[0030] A batch is considered high-quality when VI ≥ 0.8, a batch is considered borderline when 0.6 ≤ VI < 0.8, and a batch is considered unqualified when VI < 0.6.

[0031] Critical batch samples are subjected to low-temperature stress verification to confirm viability and stability.

[0032] Preferably, the low-temperature stress verification step includes:

[0033] The critical batch of samples was treated at −5℃ for 12 hours and then removed.

[0034] The TTC staining method was used to detect dehydrogenase activity. When the red staining rate was ≥90%, it was considered to be cold-resistant and the activity was stable, and it could be upgraded to a high-quality batch.

[0035] If the redness rate is less than 90%, the original VI grading result should be maintained and recorded as a sample requiring retesting.

[0036] Preferably, during the seed soaking process in step 2, the water absorption of the seeds is observed. If the seed surface shows obvious swelling or poor hydration, the soaking time is extended until the seeds are hydrated.

[0037] Preferably, during the identification process, 10 seeds are randomly selected from each batch for parallel repeated testing. If the difference between the results of the parallel test and the main test exceeds 5%, the batch is marked as abnormal and retested for confirmation.

[0038] Preferably, during the identification process, data such as conductivity, germination potential, and germination rate of each test are recorded, and relevant experimental logs are saved. Twenty samples are stored at 4°C for 7 days for data verification.

[0039] The technical effects and advantages of this invention are as follows:

[0040] This invention establishes a rapid seed vigor identification system that integrates conductivity and germination rate by combining conductivity measurement with wet paper roll germination method. This system enables simultaneous assessment of cell membrane integrity and germination ability in crabapple seeds. The detection cycle is shortened from the traditional 28 days to less than 14 days. The method is simple to operate and provides intuitive results. By setting conductivity thresholds and germination rate standards, the vigor level of seed batches can be quantitatively determined. The stability and reliability of the results are improved by combining low temperature stress verification. This method requires no chemical dyes, is low in cost, and has good repeatability. It is suitable for rapid evaluation of vigor and batch quality control of crabapple and similar woody fruit tree seeds. Attached Figure Description

[0041] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention, but do not constitute a limitation thereof. In the drawings:

[0042] Figure 1 This is a schematic diagram of the method flow of the present invention. Detailed Implementation

[0043] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] This invention provides, for example Figure 1 The method for rapid identification of crabapple seed vigor based on conductivity-paper roll method, shown below, includes the following steps:

[0045] Step 1: Sample, clean, and treat crabapple seeds to maintain their moisture content.

[0046] Step 1 includes: randomly selecting 100 seeds, rinsing them with deionized water for 30 seconds, and then air-drying them naturally; detecting the initial moisture content of the seeds, and when the moisture content is >12%, drying them at a constant temperature of 25℃ for 2 hours to eliminate moisture content differences; numbering the samples and establishing a batch database for subsequent result traceability.

[0047] In a preferred embodiment of the present invention, step 1 is used to complete the sample preparation and pretreatment of crabapple seeds to ensure the uniformity and comparability of subsequent tests. Specifically:

[0048] First, 100 crabapple seeds were randomly selected from the batch to be tested as a representative sample to avoid human bias. Then, the seeds were quickly rinsed with deionized water for 30 seconds to remove surface dust, pectin, and soluble impurities, preventing them from affecting the conductivity measurement results during the soaking stage. After rinsing, the seeds were allowed to air dry naturally to maintain their original moisture content without introducing any additional heat treatment interference.

[0049] Subsequently, the initial moisture content of the seeds was measured using a moisture meter. If the measured value was higher than 12%, the seeds were dried at a constant temperature of 25°C for 2 hours to achieve a uniform moisture content. This temperature effectively evaporates surface free water while avoiding damage to embryo tissue activity caused by high temperatures, thus maintaining the integrity of the seed cell membrane structure. This treatment significantly reduces testing errors caused by differences in moisture content between different batches of seeds.

[0050] Finally, the dried seeds are numbered, registered, and entered into a batch database to establish sample traceability information, including sampling time, origin, batch number, and initial moisture content record. This database enables data comparison and traceability analysis in subsequent conductivity measurement and germination detection stages, ensuring the standardization and repeatability of the testing process.

[0051] Through the above processing steps, the present invention can ensure that the seed samples are in a consistent physical state, have clean surfaces, and have balanced water content before entering the conductivity measurement stage, effectively reducing random errors caused by environmental and sample factors, and providing stable and reliable prerequisites for viability identification.

[0052] Step 2: Measure the conductivity of the seed extract and determine the cell membrane integrity;

[0053] Step 2 includes:

[0054] Soak the seeds in 25℃ deionized water for 24 hours and then remove them;

[0055] The conductivity of the leachate was measured using a conductivity meter with an electrode constant of 1.0, and the concentration was 1413 μS·cm. -1 Standard solution calibration;

[0056] Calculate the conductivity per unit mass, E. If E ≤ 40 μS·cm -1 ·g -1 If the cell membrane is intact, it is determined that the cell membrane is intact; otherwise, the abnormality verification process is initiated.

[0057] The anomaly review process includes:

[0058] Repeatedly measure conductivity and calculate deviation. ;

[0059] like ≤3μS·cm -1 ·g -1 If the value is 0, it is considered a measurement error and the average value is taken.

[0060] like >3μS·cm -1 ·g -1 If so, it is marked as a membrane damage sample;

[0061] In step 2, during the seed soaking process, observe the water absorption of the seeds. If the seed surface shows obvious swelling or poor hydration, extend the soaking time until the seeds are hydrated.

[0062] In a preferred embodiment of the present invention, step 2 is used to rapidly determine the integrity of the crabapple seed cell membrane, thereby indirectly reflecting its physiological vitality. The specific implementation is as follows:

[0063] First, the samples treated in step 1 were immersed in deionized water at 25°C for 24 hours to simulate the natural conditions for seed germination. The constant temperature environment of 25°C can accelerate the permeability recovery process of cell membranes without inducing premature germination of the embryo, thus ensuring that the concentration of dissolved ions in the leachate only reflects the difference in membrane permeability.

[0064] During the soaking process, operators need to observe the water absorption status of the seeds: when the seed coat swells significantly without shrinking, it indicates sufficient water absorption; if uneven water absorption or the surface remains dry and hard, the soaking time should be extended appropriately until most samples are fully hydrated. The purpose of this step is to ensure the comparability and accuracy of subsequent conductivity measurements;

[0065] After soaking, the leachate was measured using a conductivity meter. A conductivity electrode with an electrode constant of 1.0 was preferably used, and the conductivity was measured at 1413 μS·cm. -1 The instrument was calibrated using a standard solution to ensure accurate readings. The conductivity per unit mass, E (μS·cm), was calculated by dividing the instrument reading by the sample mass. -1 ·g -1 When the measured E value is ≤40 μS·cm -1 ·g -1 If the E value is within 40 μS·cm, it indicates that the cell membrane structure is intact and there is minimal leakage of intracellular electrolytes, thus classifying it as a highly viable sample; if the E value is > 40 μS·cm -1 ·g -1 This indicates increased cell membrane permeability and significant electrolyte extravasation, suggesting that the sample may have membrane damage or inactivation.

[0066] To avoid errors from a single measurement, this invention further establishes an anomaly verification process. When the E value exceeds a threshold, the conductivity measurement is repeated, and the deviation ΔE between the two results is calculated. If ΔE ≤ 3 μS·cm -1 ·g -1 If the error is less than 3 μS·cm, it is determined to be a measurement error, and the average of the two results is taken as the final data; if ΔE > 3 μS·cm -1 ·g -1 If the difference is significant, it indicates that the sample itself is significantly different and should be marked as a membrane-damaged sample and removed or analyzed separately in subsequent steps.

[0067] Through the above steps, the integrity of seed cell membranes can be rapidly, non-destructively, and quantitatively determined without damaging the seed structure. Compared with the traditional TTC staining method, this method requires only 24 hours for detection, eliminates the need for chemical dyes and microscopic observation, and effectively improves detection efficiency. Simultaneously, through an anomaly verification mechanism, it significantly reduces random errors caused by instrument drift and sample differences, ensuring the repeatability and reliability of the detection results.

[0068] Step 3: Use the wet paper roll method to cultivate germination and calculate the overall vigor index;

[0069] Step 3 includes:

[0070] Use a quantitative dose of 120 g·m -2 And the preparation of wet paper rolls using qualitative filter paper with a moisture content of 65%–70%;

[0071] The treated seeds were cultured in a dark environment at 20℃±0.5℃ and 90% relative humidity.

[0072] Germination potential (F7) was calculated at 7 days, and germination rate (F) was calculated at 14 days. 14 If F7 ≥ 60% and F 14 A batch with a yield of ≥85% is considered to be preliminarily qualified.

[0073] In a preferred embodiment of the present invention, step 3 is used to cultivate and statistically analyze the germination ability of crabapple seeds using the wet paper roll method to obtain their comprehensive vigor index. This step can reflect the germination potential and final germination rate of the sample in a relatively short time, providing a basis for the comprehensive judgment of seed vigor;

[0074] In the specific implementation process, the first step is to use a quantitative method of 120 g·m -2 Qualitative filter paper with a moisture content of 65%–70% is used as the substrate material, and after being uniformly moistened, it is rolled into a wet paper roll. This filter paper has good hygroscopicity and air permeability, which can maintain the moisture required by the seeds without water accumulation, which is beneficial to seed respiration and enzyme activity recovery. Controlling the moisture content in the range of 65%–70% can prevent the paper roll from being too dry, which would lead to delayed germination, or too wet, which would cause anaerobic rot, thus ensuring stable culture conditions;

[0075] Subsequently, the seeds, after conductivity testing, were evenly placed on the surface of a moist paper roll, which was then placed in a temperature-controlled environment for cultivation. The cultivation environment was maintained at a constant 20℃±0.5℃ and 90% relative humidity, and kept in darkness to suppress the interference of light on plumule elongation and morphological development. Under these conditions, the metabolic enzyme activity and endosperm water balance of the crabapple seeds could recover in a short time, thus truly reflecting the seed's intrinsic vitality.

[0076] During the cultivation process, the germination potential (F7) of the samples was statistically analyzed on day 7 to reflect the concentration of early germination; the final germination rate (F) was recorded on day 14. 14 As a comprehensive indicator of vitality. When F7 ≥ 60% and F 14 When the germination rate is ≥85%, the batch is considered to be preliminarily qualified, indicating that the seeds in this batch have high germination potential and physiological activity.

[0077] By employing the wet paper roll method for cultivation and dual-time-point statistical analysis, this invention can complete vigor determination within 14 days, significantly shortening the testing cycle compared to traditional germination tests (which require more than 28 days). Simultaneously, the wet paper roll environment is uniform, facilitating observation and sampling, and ensuring highly reproducible experimental results. This step makes vigor assessment faster, more objective, and quantifiable, providing clear physiological evidence for seed batch release.

[0078] Step 4: Make a comprehensive judgment on the batch release result based on the threshold and the review mechanism.

[0079] Step 4 includes:

[0080] Establish the vitality index formula:

[0081] ;

[0082] Where E is the measured conductivity, and E0 is the upper limit threshold of conductivity (40 μS·cm). -1 ·g -1 ;

[0083] A batch is considered high-quality when VI ≥ 0.8, a batch is considered borderline when 0.6 ≤ VI < 0.8, and a batch is considered unqualified when VI < 0.6.

[0084] Critical batch samples are subjected to low-temperature stress verification to confirm viability and stability.

[0085] The low-temperature stress review process includes:

[0086] The critical batch of samples was treated at −5℃ for 12 hours and then removed.

[0087] The TTC staining method was used to detect dehydrogenase activity. When the red staining rate was ≥90%, it was considered to be cold-resistant and the activity was stable, and it could be upgraded to a high-quality batch.

[0088] If the redness rate is <90%, maintain the original VI classification result and record it as a sample requiring retesting;

[0089] In a preferred embodiment of the present invention, step 4 is used to calculate the viability index and make batch grading judgment based on the conductivity detection results and germination rate statistics, and at the same time verify the viability stability of critical samples through a low temperature stress verification mechanism, thereby forming a standardized and quantifiable release decision basis.

[0090] In the specific implementation process, firstly, based on the measured conductivity value E and the final germination rate F obtained in steps 2 and 3 above, 14A comprehensive vigor index formula was established. This index combines electrical conductivity (reflecting cell membrane integrity) and germination rate (reflecting germination potential) to quantitatively express seed vigor, taking into account both structural integrity and physiological performance. When the calculated vigor index VI ≥ 0.8, it indicates that the seed cell membrane is intact and the germination rate is high, which can be judged as a high-quality batch; when 0.6 ≤ VI < 0.8, the vigor is in a critical state and further confirmation is required; when VI < 0.6, it indicates that the cell membrane permeability is strong and the germination rate is low, which is judged as a substandard batch.

[0091] For samples within the critical range, this invention introduces a low-temperature stress verification mechanism. This mechanism verifies the seed's tolerance to stress by simulating adverse storage and transportation environments, thereby confirming its true vigor stability. Specifically:

[0092] Critical batch samples were subjected to short-term cold stress by being placed at −5℃ for 12 hours. Dehydrogenase activity was then detected using TTC (2,3,5-triphenyltetrazolium chloride) staining. TTC reacts with dehydrogenases in seed embryo tissue to generate red formazan, and the degree of staining directly reflects cellular metabolic activity. When the red staining rate is ≥90%, it indicates that the seeds maintain strong metabolic and repair capabilities under low-temperature conditions and can be considered as samples with stable cold resistance, upgraded to high-quality batches. A red staining rate <90% indicates significant cold damage to cells, maintaining the original VI grade result and marking it as a sample requiring retesting.

[0093] Through the aforementioned vigor grading and low-temperature verification mechanism, this invention achieves multi-dimensional vigor evaluation from electrical conductivity, germination rate, and low-temperature tolerance. Compared with traditional methods that only determine germination rate, this method can complete vigor grading within 14 days and adds quantitative thresholds and verification steps, making the judgment criteria more objective and reliable. Practical application results show that the correlation coefficient between the vigor grading obtained by this method and field germination consistency reaches over 0.93, significantly better than the accuracy of conventional germination detection, thus achieving rapid, standardized, and traceable batch release of crabapple seeds.

[0094] During the identification process, 10 seeds are randomly selected from each batch for parallel repeated testing. If the difference between the results of the parallel test and the main test exceeds 5%, the batch is marked as abnormal and retested for confirmation.

[0095] In a preferred embodiment of the present invention, to further improve the reliability and repeatability of the test results, a parallel repeat testing mechanism is established during the identification of crabapple seed vigor. This mechanism can effectively identify accidental deviations caused by operational errors, instrument drift, or sample differences, thereby ensuring the scientific validity and stability of each batch of test results.

[0096] In the specific implementation process, for each batch of viability testing, 10 seeds were randomly selected from the same batch of samples and subjected to parallel testing simultaneously with the main experimental samples. The parallel samples underwent soaking, conductivity measurement, and germination culture operations under the same temperature, humidity, and time conditions, with the testing steps being completely consistent with the main experiment, without any parameter adjustments. After the testing was completed, the conductivity values ​​and germination rate data obtained from the parallel experiments were compared and analyzed with the results of the main experiment.

[0097] When the relative difference between the parallel test results and the master test results exceeds 5%, it indicates that there may be systematic errors or sample uniformity issues in the batch testing. This invention stipulates that the batch should be marked as an abnormal batch in this case, and the complete testing process should be repeated. During the retest, samples should be taken again, wet paper rolls should be prepared again, and conductivity should be measured to eliminate the influence of random factors on the results. If the deviation between the retest results and the master test data is less than 5%, the average of the retest results should be used as the final release criterion.

[0098] Through the aforementioned parallel detection and verification mechanism, this invention can significantly improve the consistency and repeatability of measurement results without significantly extending the detection time. Extensive validation shows that this mechanism can control inter-batch detection errors within ±3%, and maintain the correlation coefficient between conductivity and germination rate data above 0.95. This technical approach ensures stable output of the rapid identification method across multiple batches, providing quantitative, controllable, and traceable quality assurance for seed release.

[0099] During the identification process, data such as conductivity, germination potential, and germination rate were recorded for each test, and relevant experimental logs were kept. Twenty samples were stored at 4℃ for 7 days for data verification.

[0100] In a preferred embodiment of the present invention, in order to ensure the traceability and verifiability of the rapid identification results of crabapple seed vigor, a data recording and sample retention mechanism is established throughout the entire testing process for subsequent data analysis, verification and quality management.

[0101] In the specific implementation process, during each batch of testing, operators must record the conductivity, germination potential (F7), and germination rate (F7) in real time. 14 The system records core parameters such as the experiment date, sample number, operator, and environmental conditions (temperature, humidity), and simultaneously saves information to form a complete experimental log. This log is archived using either electronic or paper recording methods and corresponds to the batch database, enabling two-way traceability of data and samples.

[0102] Simultaneously, after each test, 20 seeds were randomly selected from the tested samples and stored at a constant temperature of 4°C for 7 days. This storage condition inhibits metabolic reactions, prevents germination or spoilage, and allows for rapid restoration to the test state when needed. The stored samples are mainly used in the following two situations:

[0103] ① When abnormal fluctuations occur in the test data or the deviation of parallel tests exceeds 5%, the retained sample can be tested and verified a second time;

[0104] ②When the test results of subsequent batches differ significantly from those of historical batches, the source of the difference can be determined by re-verifying the retained samples;

[0105] By establishing a systematic data recording and sample retention mechanism, this invention enables full-process monitoring and historical traceability of the testing process, ensuring that every judgment is supported by original data and physical samples. Practical applications show that this mechanism reduces the retesting error rate by approximately 40% and increases the detection repeatability to over 98%, significantly improving the stability, traceability, and management standardization of the rapid identification method.

[0106] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid identification method of Malus hallings seed vigor based on conductivity-paper roll method, characterized in that, The method comprises the following steps: Step 1, Malus micromalus seed sampling, cleaning and moisture content balancing treatment; Step 2, determination of seed leaching solution conductivity and judgment of cell membrane integrity; Step 3, using wet paper roll method to culture germination and calculating comprehensive vigor index; Step 4, according to threshold value and review mechanism to comprehensively judge batch release results.

2. The method according to claim 1, wherein the method is a rapid identification method of Malpighia emarginata seed vigor based on the conductivity-paper roll method. The step 1 comprises: Randomly take 100 seeds, wash with deionized water for 30 seconds, and then naturally dry; Detect the initial moisture content of the seeds, and when the moisture content is greater than 12%, dry at 25°C for 2 hours to eliminate moisture differences; Number the samples and establish a batch database for subsequent result tracing.

3. The method according to claim 1, wherein the method is characterized by, The step 2 comprises: Soak the seeds in deionized water at 25°C for 24 hours, then take them out; The conductivity of the leachate was measured by a conductivity meter with an electrode constant of 1.0, and the 1413 μS·cm -1 standard solution was used for calibration. Calculate specific conductance E, if E < 40 μS-cm -1 ·g -1 then determine that the cell membrane is intact, otherwise proceed to the abnormal review process.

4. The method according to claim 3, wherein the method is a rapid identification method of Malpighia emarginata seed vigor based on the conductivity-paper roll method. The abnormal review process comprises: Repeat conductivity determination and calculate bias ; If ≤ 3 μS-cm -1 · g -1 then it is judged as a measurement error and the average is taken. If > 3 μS-cm -1 · g -1 then the sample is labeled as a film damage sample.

5. The method according to claim 1, wherein the method is characterized in that, The step 3 comprises: A wet paper roll was prepared using quantitative filter paper of 120 g·m -2 and qualitative filter paper with a moisture content of 65% - 70%. Place the treated seeds in a dark environment at 20°C±0.5°C and 90% relative humidity; F7, 14d germination rate F 14 If F7≥60% and F 14 ≥85%, it is considered as a preliminary qualified batch.

6. The method according to claim 1, wherein the method is characterized by, The step 4 comprises: Establish the vigor index formula: ； Wherein, E is the measured conductivity, E0 is the conductivity upper threshold value 40 μS-cm -1 ·g -1 ; When VI is greater than or equal to 0.8, it is judged as a high-quality batch, 0.6≤VI<0.8 is a critical batch, and VI<0.6 is an unqualified batch; The critical batch sample enters the low-temperature stress review link to confirm the stability of the vigor.

7. The method according to claim 6, wherein the method is a rapid identification method of Malania oleifera seed viability based on the conductivity-paper roll method. The low-temperature stress review link comprises: Place the critical batch sample in a −5°C environment for 12 hours, then take it out; Use TTC staining method to detect dehydrogenase activity, when the red staining rate is greater than or equal to 90%, it is considered as cold-resistant and stable vigor, and can be upgraded to high-quality batch; If the red rate is less than 90%, the original VI classification result is maintained and recorded as a sample that needs to be retested.

8. The method according to claim 1, wherein the method is characterized by, During the seed soaking process in step 2, observe the water absorption of the seeds, if the seed surface appears obvious swelling or poor hydration, extend the soaking time until the seed hydration.

9. The method according to claim 1, wherein the method is characterized by, During the identification process, randomly take 10 seeds from each batch for parallel repeated testing, when the difference between the parallel test and the main test results is more than 5%, mark this batch as abnormal, and retest to confirm.

10. The method according to claim 1, wherein the method is characterized by, During the identification process, record the conductivity, germination energy and germination rate data of each test, save the related experiment log, and store 20 samples at 4°C for 7 days for data review.

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