Baby formula milk powder simulating breast milk as well as preparation method and application of baby formula milk powder

Abstract

The invention discloses infant formula milk powder simulating breast milk as well as a preparation method and application of the infant formula milk powder, and belongs to the technical field of food. The infant formula milk powder comprises the following raw materials in parts by weight: raw sheep milk, a whey protein compound, compound grease, compound vitamins, compound minerals, prebiotics, probiotics, choline chloride, taurine, nucleotide, docosahexaenoic acid and arachidonic acid. The whey protein compound is subjected to enzymolysis by adopting a specific enzyme, so that the average molecular weight of whey protein is reduced, the molecular mass distribution of the whey protein is basically consistent with that of whey protein in breast milk, and the whey protein compound is combined with raw sheep milk to be closer to the structure and proportion of the whey protein in the breast milk, and has the effects of obviously improving immunity, resisting allergy and being easy to digest and absorb.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of food technology, and particularly relates to a baby formula milk powder simulating breast milk and a preparation method and application thereof. BACKGROUND

[0002] Breast milk is the most ideal natural food for babies, which contains a variety of nutrients with appropriate proportions and unique biological activity functions, and can meet the nutritional needs of baby growth and development, and provide immune protection and promote the development of intestinal health for babies. However, due to insufficient breast milk supply, physical condition limitations of the mother and other reasons, baby milk powder becomes an important substitute for breast milk. Traditional baby milk powder is mostly made of cow milk or goat milk, and the whey protein composition thereof is significantly different from that of breast milk, which leads to the mismatch of the amino acid pattern of the milk powder and the needs of babies, and is prone to cause infantile allergy; the whey protein in the traditional milk powder has a large molecular weight, and the digestive system of babies has not yet developed completely, so that the babies are difficult to fully digest and absorb, and are prone to have digestive discomfort symptoms such as abdominal distension and diarrhea.

[0003] Some research and development of milk powder simulating the whey protein of breast milk have appeared in the prior art, for example, adding a single whey protein component (such as lactoferrin) to improve the biological activity of the milk powder. These technologies still have obvious deficiencies: first, only one or several whey protein components are adjusted, the overall proportion of the whey protein of breast milk is not accurately simulated, and the needs of babies for a variety of biological active proteins cannot be met; second, the molecular weight distribution of the whey protein is uneven, and the content of some small molecular peptides is too high to cause intestinal irritation of babies, or the content of large molecular peptides is too high to affect the digestion and absorption; third, the synergistic effect of the whey protein and other nutrients is not considered, so that the overall nutritional efficacy of the milk powder cannot reach the level of breast milk. Therefore, it is a key requirement in the field of infant formula food to develop a baby milk powder which can accurately simulate the structure and proportion of the whey protein of breast milk, and has balanced nutrition, digestion and absorption, low allergy and improved immunity. SUMMARY

[0004] In view of the above, the purpose of the present application is to provide a baby formula milk powder simulating breast milk and a preparation method and application thereof, which accurately regulates the proportion of whey protein components, optimizes the molecular structure by combining with enzyme modification technology, and realizes the overall simulation of the whey protein system of breast milk by compounding functional adjuvants, so as to meet the needs of baby growth and development, and has the characteristics of significantly improving immunity, low allergy and easy digestion.

[0005] In order to achieve the above-mentioned purpose of the application, the present application provides the following technical solutions: The application provides a kind of infant formula milk powder simulating breast milk, comprising the following raw materials by weight: raw sheep milk 55-65 parts, whey protein complex 5-15 parts, compound oil 1-4 parts, complex vitamins 0.03-0.2 parts, complex minerals 0.03-0.2 parts, prebiotics 3-6 parts, probiotics 0.06-0.2 parts, choline chloride 0.005-0.015 parts, taurine 0.003-0.01 parts, nucleotides 0.004-0.01 parts, docosahexaenoic acid 0.05-0.2 parts and arachidonic acid 0.05-0.2 parts; when preparing the whey protein complex, the raw materials used include α-lactalbumin, β-lactoglobulin, lactoferrin, immunoglobulin, and the mass ratio of the above raw materials is (2.8-3.2):(1.8-2.2):(0.08-0.12):(0.04-0.06), respectively.

[0006] Preferably, the compound oil includes soybean oil, olive oil, coconut oil, and medium-chain fatty acid MCT, and the mass ratio of the above raw materials is (6.5-8.5):(3.5-5.5):(1.5-3.5):(1-3), respectively.

[0007] Preferably, the probiotics include Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716, and Lactobacillus helveticus R0052, and the mass ratio of the above raw materials is (0.5-1.5):(1.5-2.5):(0.5-1.5), respectively.

[0008] Preferably, the prebiotics include 2'-fucosyllactose, galacto-oligosaccharide, and lactulose, and the mass ratio of the three is (0.5-1.5):(2.5-3.5):(2.5-3.5), respectively.

[0009] The application provides a preparation method of the infant formula milk powder, comprising the following steps: mixing and enzymatically hydrolyzing a whey protein complex raw material solution with a complex protease to obtain whey protein complex; uniformly mixing raw sheep milk, whey protein complex, compound oil, complex vitamins, complex minerals, choline chloride, and taurine, adjusting pH, and then homogenizing, sterilizing, and concentrating to obtain milk powder; and uniformly mixing the milk powder with prebiotics, probiotics, docosahexaenoic acid, arachidonic acid, and nucleotides.

[0010] Preferably, the complex protease includes trypsin and papain, and the addition amount of the complex protease is 0.2-0.6 wt% of the total mass of the whey protein complex raw material solution.

[0011] Preferably, the mass ratio of the trypsin to the papain is (1-3):(0.5-1.5); the enzyme activity of the trypsin is 3500-4500 U / g, and the enzyme activity of the papain is 90-110 thousand U / g; the enzymolysis conditions include 45-60 DEG C and 1-3 h.

[0012] Preferably, the pH is 6.8-7.2.

[0013] Preferably, the homogenization conditions include 25-35 MPa, 35-45 DEG C and 1-4 times.

[0014] The application provides application of the infant formula milk powder or the infant formula milk powder prepared by the preparation method in preparation of an immune-enhancing and anti-allergic product.

[0015] The application has the following beneficial effects: The application realizes comprehensive bionics of the whey protein system of breast milk by precisely regulating the proportion of whey protein components, optimizing the molecular structure by combining with enzyme modification technology, and compounding functional excipients, so that the growth and development requirements of infants are met, the immune-enhancing, low-allergic and easy-digesting characteristics are achieved, and the problems of unbalanced whey protein proportion and large molecular structure difference in traditional milk powder are solved.

[0016] The milk powder prepared by the application has excellent digestibility and low allergenicity, the average molecular weight of the whey protein after enzyme hydrolysis is matched with the substrate characteristics of the digestive enzymes in the intestinal tract of infants, the whey protein is easy to be digested and absorbed by infants, the symptoms of indigestion such as abdominal distension and diarrhea can be effectively reduced, and the allergenicity is reduced. Meanwhile, the contents of lactoferrin and immunoglobulin in the milk powder are close to those in breast milk, and the two components play the roles of antibacterial, anti-inflammatory and immune regulation in cooperation. The milk powder not only optimizes the whey protein system, but also reasonably compiles components such as vegetable oil, probiotics, prebiotics, vitamins and minerals, so that the nutritional composition of the milk powder is highly close to that of breast milk, and the comprehensive requirements of infants for growth and development are met.

[0017] The application adopts advanced processes such as ultrasonic pretreatment, ultra-high temperature instantaneous sterilization and spray drying, which can not only ensure the safety and stability of the product, but also maximize the retention of the biological activity of nutritional components, and solves the problems of large nutritional loss and low survival rate of probiotics in the traditional preparation process. DETAILED DESCRIPTION

[0018] The present application provides a kind of infant formula milk powder simulating breast milk, including the following weight parts raw materials: raw sheep milk 55-65 parts, whey protein complex 5-15 parts, compound oil 1-4 parts, complex vitamin 0.03-0.2 parts, complex mineral 0.03-0.2 parts, prebiotic 3-6 parts, probiotic 0.06-0.2 parts, choline chloride 0.005-0.015 parts, taurine 0.003-0.01 parts, nucleotide 0.004-0.01 parts, docosahexaenoic acid 0.05-0.2 parts and arachidonic acid 0.05-0.2 parts; preferably including the following weight parts raw materials: raw sheep milk 57-63 parts, whey protein complex 7-13 parts, compound oil 2-3 parts, complex vitamin 0.04-0.18 parts, complex mineral 0.04-0.18 parts, prebiotic 4-5 parts, probiotic 0.08-0.18 parts, choline chloride 0.006-0.013 parts, taurine 0.004-0.009 parts, nucleotide 0.005-0.009 parts, docosahexaenoic acid 0.06-0.18 parts and arachidonic acid 0.06-0.18 parts.The above-mentioned raw materials of the present application are all edible raw materials for infants if not specially stated, and all come from commercially available products well known in the art.

[0019] In the present application, the raw materials used in the preparation of the whey protein complex include α-lactalbumin, β-lactoglobulin, lactoferrin, immunoglobulin; preferably the raw materials are made of α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin.The mass ratio of α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin is (2.8-3.2):(1.8-2.2):(0.08-0.12):(0.04-0.06), preferably (2.9-3.1):(1.9-2.1):(0.09-0.11):(0.05-0.058).The above-mentioned raw materials of the present application are all commercially available products well known in the art if not specially stated.

[0020] In the present application, the compound oil includes soybean oil, olive oil, coconut oil, medium-chain fatty acid MCT, and is preferably made of soybean oil, olive oil, coconut oil and medium-chain fatty acid MCT.The mass ratio of soybean oil, olive oil, coconut oil and medium-chain fatty acid MCT is (6.5-8.5):(3.5-5.5):(1.5-3.5):(1-3), preferably (6.8-8.3):(3.8-5.3):(1.8-3.3):(1.2-2.8).The above-mentioned raw materials of the present application are all edible raw materials for infants if not specially stated, and all come from commercially available products well known in the art.

[0021] In the present application, the probiotics include Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052, preferably are made of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052. The mass ratio of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052 in the present application is (0.5-1.5):(1.5-2.5):(0.5-1.5), preferably (0.8-1.3):(1.8-2.3):(0.8-1.3). The viable count ratio of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052 in the present application is (0.8-1.6):(1-3):(0.5-1.5), preferably (0.9-1.5):(1.5-2.5):(0.8-1.3).

[0022] The strains selected in the present application are safe and effective probiotic strains in infant food, and the combination of the three selected strains can regulate the intestinal flora balance of infants, enhance the intestinal barrier function, be beneficial to the absorption of milk powder raw materials, improve the immunity of the body, and also have low allergenicity when compounded with other raw materials. The above-mentioned strains in the present application are all from commercially available strains well known in the art.

[0023] In the present application, the prebiotics include 2'-fucosyllactose, galacto-oligosaccharide and lactulose, preferably are composed of 2'-fucosyllactose, galacto-oligosaccharide and lactulose. The mass ratio of 2'-fucosyllactose, galacto-oligosaccharide and lactulose in the present application is (0.5-1.5):(2.5-3.5):(2.5-3.5) in turn, preferably (0.8-1.3):(2.8-3.3):(2.8-3.3). The combination of the above-mentioned three raw materials in the present application can serve as a carbon source for probiotics, promote the proliferation of probiotics, inhibit the growth of harmful bacteria, and synergistically play the intestinal health care role with probiotics. The above-mentioned raw materials in the present application are all edible raw materials for infants, and are all commercially available products well known in the art.

[0024] The present application also provides a preparation method of the infant formula milk powder, which comprises the following steps: mixing and enzymatically hydrolyzing a whey protein complex raw material solution with a complex protease to obtain a whey protein complex; uniformly mixing raw goat milk, the whey protein complex, compound oil, complex vitamins, complex minerals, choline chloride and taurine, and then homogenizing, sterilizing and concentrating to obtain milk powder; and uniformly mixing the milk powder with prebiotics, probiotics, docosahexaenoic acid, arachidonic acid and nucleotides. The preparation method set in the present application retains the effective components and functions of the raw materials, so that the raw materials synergistically play the effects, and the prepared milk powder is uniform in particles and free of odor.

[0025] In the present application, the complex protease comprises trypsin and papain, and the adding amount of the complex protease is 0.2-0.6 wt% of the total mass of the raw material solution of the whey protein complex, preferably 0.4 wt%. The complex protease in the present application is composed of trypsin and papain; the mass ratio of the trypsin to the papain is (1-3):(0.5-1.5), preferably 2:1; the enzyme activity of the trypsin is 3500-4500 U / g, preferably 4000 U / g, and the enzyme activity of the papain is 90-110 thousand U / g, preferably 100 thousand U / g. The enzymolysis conditions in the present application include 45-60℃ and 1-3 h, preferably 50℃ and 2 h. The enzymolysis product obtained by using the specific enzyme can ensure that the amino acid pattern of the milk powder matches the needs of infants, and at the same time, provides sufficient immune active substances for infants. The enzymolysis in the present application is carried out under specific process parameters, so that the average molecular weight of the whey protein is basically consistent with the molecular weight distribution of the whey protein in breast milk, which can not only avoid the problem that large molecular proteins are difficult to digest, but also prevent intestinal irritation caused by excessive small molecular peptides, while retaining the biological activity of the whey protein.

[0026] In the present application, the homogenization conditions include 25-35 MPa, 35-45℃, and 1-4 times, preferably 30 MPa, 40℃, and 2 times. The homogenization treatment in the present application can refine the fat globules and protein particles in the emulsion, improve the solubility and stability of the milk powder, and avoid the stratification phenomenon.

[0027] In the present application, the pH is 6.8-7.2, preferably pH 7.0. The pH is adjusted by using the pH adjuster well known in the art. The pH in the present application is close to that of breast milk, which can reduce the stimulation to the intestinal tract of infants.

[0028] The present application provides the application of the infant formula milk powder obtained by the preparation method of the infant formula milk powder in the preparation of immune-enhancing and low-allergy products.

[0029] The technical solutions provided by the present application will be described in detail below in conjunction with the embodiments, but they should not be understood as limiting the scope of protection of the present application.

[0030] In the following examples, if not specifically stated, the methods are conventional methods.

[0031] In the following examples, the materials, reagents, etc. used, if not specifically stated, can be obtained from commercial channels.

[0032] Example 1 A kind of infant formula milk powder simulating breast milk is made from the following raw materials by mass fraction: raw sheep milk (from Asaf milk sheep, Spanish pasture) 60 parts, whey protein complex 10 parts, compound oil 2.5 parts, complex vitamins 0.08 parts, complex minerals 0.09 parts, prebiotics 4 parts, probiotics 0.09 parts, choline chloride 0.009 parts, taurine 0.005 parts, nucleotides 0.006 parts, docosahexaenoic acid 0.15 parts and arachidonic acid 0.15 parts.

[0033] The whey protein complex is prepared using raw materials consisting of α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin in a mass ratio of 3:2:0.1:0.05.

[0034] The compound oil is composed of soybean oil, olive oil, coconut oil and medium-chain fatty acid MCT in a mass ratio of 7.5:4.5:2.5:2.

[0035] The complex minerals are purchased from Beijing Jin Kang Pu Food Technology Co., Ltd., and each gram of the complex minerals comprises: copper 3550 μg, iron 50 mg, zinc 47 mg, manganese 240 μg, iodine 940 μg and selenium 235 μg; the complex vitamins are purchased from Beijing Jin Kang Pu Food Technology Co., Ltd., and each gram of the complex vitamins comprises: vitamin A 2350 μg RE, vitamin D 80 μg, vitamin E 53 mg α-TE, vitamin K1 350 μg, vitamin B1 3550 μg, vitamin B2 2350 μg, vitamin B6 800 μg, vitamin B12 3.1 μg, biotin 45 μg, nicotinamide 19000 μg, folic acid 535 μg, pantothenic acid 15500 μg and vitamin C 475 mg. The probiotics are composed of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052 in a mass ratio of 1:2:1 and a live bacteria ratio of 1.2:1.8:1.

[0036] The prebiotics are composed of 2'-fucosyllactose, galactooligosaccharide and lactulose in a mass ratio of 1:3:3.

[0037] A preparation method of an infant formula milk powder simulating breast milk, comprising the following steps: (1) Preparation of whey protein complex: weigh α-lactalbumin, β-lactoglobulin, lactoferrin, immunoglobulin according to the proportion, mix them uniformly, then add deionized water to prepare a whey protein solution with a mass concentration of 13%; the whey protein solution is subjected to ultrasonic pretreatment (power 350 W, time 12 min), then complex protease (trypsin (4000 U / g): papain (100000 U / g)) is added in a mass ratio of 2:1, the enzyme addition amount is 0.4 wt% of the total mass of the whey protein complex, and the enzyme hydrolysis is carried out at 50°C for 2 h; after the enzyme hydrolysis is completed, the enzyme is inactivated at 90°C for 12 min, and then cooled to room temperature to obtain the whey protein complex.

[0038] (2) The raw goat milk, whey protein complex, compound oil, galacto-oligosaccharide, complex vitamin, complex mineral substance, choline chloride and taurine are mixed uniformly and completely dissolved according to the weight ratio, and the mixture is homogenized at 15000 r / min for 30 min, the temperature is controlled at 30°C, the mixing time is 20 min, then the pH is adjusted to 7.0 to obtain a mixed liquid.

[0039] (3) The mixed liquid is homogenized at 30 MPa and 40°C, and the homogenization is repeated twice; the mixed emulsion after homogenization is subjected to ultra-high temperature instant sterilization (UHT) at 121°C for 4 s; then the emulsion is concentrated to 30% at 45°C and 150 MPa; the concentrated liquid is spray dried, the inlet air temperature is set to 150°C, the outlet air temperature is set to 100°C, and the feeding speed is set to 0.8 L / h; finally, the dried milk powder is sieved through a 35-mesh sieve to obtain the milk powder.

[0040] (4) The milk powder is uniformly mixed with prebiotics, probiotics, docosahexaenoic acid, arachidonic acid and nucleotides under the conditions of a temperature of 25°C and a relative humidity of 30% to obtain an infant formula milk powder (viable bacterial count 1.8×10 6 / g).

[0041] Example 2 An infant formula milk powder simulating breast milk is prepared from the following raw materials by mass: raw goat milk 55 parts, whey protein complex 5 parts, compound oil 1 part, complex vitamin 0.03 part, complex mineral substance 0.03 part, prebiotics 3 parts, probiotics 0.06 part, choline chloride 0.005 part, taurine 0.003 part, nucleotides 0.004 part, docosahexaenoic acid 0.05 part and arachidonic acid 0.05 part.

[0042] In the preparation of the whey protein complex, the raw materials used are composed of α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin, and the mass ratio is 2.8:1.8:0.08:0.04 in turn.

[0043] The composite oil is composed of soybean oil, olive oil, coconut oil, medium-chain fatty acid MCT, and the mass ratio is 6.5:3.6:1.5:1 in turn.

[0044] The composite minerals and the composite vitamins are the same as those in Example 1.

[0045] The probiotics are composed of Bifidobacterium longum subsp. longum BB536, Fermented Lactobacillus muci-nosus CECT5716 and Lactobacillus helveticus R0052, and the mass ratio of the three is 0.5:1.5:0.5 in turn, and the live bacteria ratio is 1:2:1 in turn.

[0046] The prebiotics are composed of 2'-fucosyllactose, galacto-oligosaccharide and lactulose, and the mass ratio of the three is 0.5:2.5:2.5 in turn.

[0047] A preparation method of a simulated breast milk infant formula milk powder, the steps are as follows: (1) Preparation of whey protein complex: weigh α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin according to the proportion, mix them uniformly, then add deionized water to prepare a whey protein solution with a mass concentration of 10%; the whey protein solution is subjected to ultrasonic pretreatment (power 330W, time 10min), then a complex protease (trypsin (3500U / g): papain (90,000U / g)) with a mass ratio of 3:1 is added, the enzyme addition amount is 0.2wt% of the total mass of the whey protein complex, and the enzyme is hydrolyzed at 45℃ for 1h; after the enzyme hydrolysis is completed, the enzyme is inactivated at 90℃ for 12min, and then cooled to room temperature to obtain the whey protein complex.

[0048] (2) The raw goat milk, whey protein complex, compound oil, composite vitamins, composite minerals, choline chloride and taurine are mixed uniformly and completely according to the above weight ratio, and the mixture is homogenized at 12000r / min for 20min, the temperature is controlled at 28℃, the mixing time is 15min, then the pH is adjusted to 6.8 to obtain a mixed liquid.

[0049] (3) The mixed liquid is homogenized at 20MPa and 45℃, repeated 2 times; the homogenized mixed emulsion is subjected to ultra-high temperature instantaneous sterilization (UHT) at 121℃ for 4s; then it is concentrated to 25% at 40℃ and 120MPa; the concentrated liquid is spray dried, the inlet air temperature is set to 145℃, the outlet air temperature is set to 100℃, and the feeding speed is set to 0.9L / h; finally, the dried milk powder is passed through a 30 mesh screen to obtain the milk powder.

[0050] (4) The milk powder is uniformly mixed with the prebiotics, probiotics, docosahexaenoic acid, arachidonic acid and nucleotides under the conditions of temperature 25℃ and relative humidity 30% to obtain the infant formula milk powder.

[0051] Example 3 A kind of infant formula milk powder simulating breast milk is made from the following raw materials by mass fraction: raw sheep milk 65 parts, whey protein complex 15 parts, compound oil 4 parts, complex vitamins 0.2 parts, complex minerals 0.15 parts, prebiotics 6 parts, probiotics 0.15 parts, choline chloride 0.013 parts, taurine 0.01 parts, nucleotides 0.001 parts, docosahexaenoic acid 0.2 parts and arachidonic acid 0.2 parts.

[0052] The whey protein complex is prepared using raw materials consisting of α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin in a mass ratio of 3.2:2.2:0.12:0.06, respectively.

[0053] The compound oil is composed of soybean oil, olive oil, coconut oil and medium-chain fatty acid MCT in a mass ratio of 8.5:5.5:3.5:3, respectively.

[0054] The complex minerals and the complex vitamins are the same as in Example 1.

[0055] The probiotics are composed of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716 and Lactobacillus helveticus R0052 in a mass ratio of 1.5:2.5:1.5, respectively, and in a viable count ratio of 0.8:1.5:0.9, respectively.

[0056] The prebiotics are composed of 2'-fucosyllactose, galactooligosaccharide and lactulose in a mass ratio of 1:3:3, respectively.

[0057] A method for preparing a kind of infant formula milk powder simulating breast milk, the steps are as follows: (1) Preparation of whey protein complex: α-lactalbumin, β-lactoglobulin, lactoferrin and immunoglobulin are weighed according to the proportion, mixed uniformly, then deionized water is added to prepare a whey protein solution with a mass concentration of 15%; the whey protein solution is subjected to ultrasonic pretreatment (power 400 W, time 15 min), then compound protease (trypsin (45,000 U / g): papain (110,000 U / g)) 1.5:1) is added, the enzyme addition amount is 0.6wt% of the total mass of the whey protein complex, and the enzyme is hydrolyzed at 55℃ for 3h; after the enzyme hydrolysis is completed, the enzyme is inactivated at 90℃ for 12 min, and then cooled to room temperature to obtain the whey protein complex.

[0058] (2) The raw sheep milk, whey protein complex, compound oil, complex vitamins, complex minerals, choline chloride and taurine are mixed uniformly and completely dissolved according to the above weight ratio, the mixed material is homogenized at 20,000 r / min for 35 min, the temperature is controlled at 30℃, the mixing time is 20 min, then the pH is adjusted to 7.2 to obtain the mixed liquid.

[0059] (3) The mixture is homogenized at 35 MPa, 45℃, repeated twice; the homogenized mixture is subjected to ultra-high temperature instant sterilization (UHT) at 121℃ for 4s; then concentrated at 50℃, 180 MPa to 35%; the concentrated solution is spray dried, with the inlet air temperature set at 180℃, the outlet air temperature at 110℃, and the feeding speed at 1L / h; finally, the dried milk powder is passed through a 40-mesh sieve to obtain the milk powder.

[0060] (4) The milk powder is uniformly mixed with prebiotics, probiotics, docosahexaenoic acid, arachidonic acid, and nucleotides at a temperature of 25℃ and a relative humidity of 30% to obtain the infant formula milk powder.

[0061] Comparative Example 1 The difference from Example 1 is that raw goat milk is replaced by raw cow milk.

[0062] Comparative Example 2 The difference from Example 1 is that the whey protein complex is composed of α-lactalbumin, β-lactoglobulin, lactoferrin, and immunoglobulin, with a mass ratio of 3:2:0.1:0.05.

[0063] In the preparation method, step (1) is omitted.

[0064] Comparative Example 3 The whey protein complex in Example 1 is replaced by hydrolyzed whey protein.

[0065] In the preparation method, step (1) is omitted.

[0066] Comparative Example 4 The difference from Example 1 is that, in the preparation of the whey protein complex, the raw materials are composed of α-lactalbumin, β-lactoglobulin, lactoferrin, and immunoglobulin, with a mass ratio of 2:2.5:0.05:0.07. The papain is replaced by neutral protease.

[0067] Comparative Example 5 The difference from Example 1 is that, in the preparation of the whey protein complex, the raw materials are composed of α-lactalbumin, β-lactoglobulin, lactoferrin, and immunoglobulin, with a mass ratio of 2.5:1.5:0.15:0.02. The papain is replaced by neutral protease.

[0068] Comparative Example 6 The difference from Example 1 is that the probiotics are composed of Bifidobacterium longum subsp. infantis CCFM1192, Lactobacillus muci-nosus CECT5716, and Lactobacillus helveticus R0052, with a mass ratio of 1:2:1.

[0069] Comparative Example 7 The difference from Example 1 is that the probiotics are composed of Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716, Lactobacillus rhamnosus HN001, and the mass ratio of the three is 1:2:1.

[0070] Experimental Example 1 The basic nutritional components of the samples were detected, and the protein content of each sample was detected by Kjeldahl determination, and the sample was repeatedly detected 10 times. The results are shown in Table 1. The total protein, whey protein and casein content of the milk powder prepared by the present application are basically consistent with those in breast milk.

[0071] Table 1: Results of basic nutritional component detection

[0072] Experimental Example 2 The milk powder prepared in Example 1 was subjected to allergen detection in Qingdao Huazhun Detection Technology Co., Ltd., and the detection results are shown in Table 2.

[0073] Table 2: Results of allergen detection

[0074] Experimental Example 3: Effect of immunity Healthy SPF level SD rat pups were selected. The test samples were the milk powder prepared in Examples 1-3 and Comparative Examples 1-7 of the present application, and the dose of the test sample for the animal experiment rats was 1.7 g / (kg·d), and the test sample was given for 28 days.

[0075] On the 14th and 28th day of the experiment, 10 rat pups were randomly selected from each group, and 5 mL of blood was collected by orbital venous blood collection method, and placed in a centrifuge tube containing sodium heparin. Centrifugation at 3000 r / min for 15 min, separate the serum and store in a-80℃ refrigerator for testing.

[0076] Immunoglobulin level detection: The contents of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) in the serum were detected by enzyme-linked immunosorbent assay (ELISA), and the operation was strictly in accordance with the corresponding kit instructions.

[0077] Cytokine detection: The levels of interleukin-2 (IL-2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the serum were detected by ELISA method, and the operation steps followed the corresponding kit instructions.

[0078] Table 3: Results of immunoglobulin detection (unit: mg / L)

[0079] Table 4 Serum cytokine levels (unit: pg / mL)

[0080] As can be seen from Table 3, from the serum immunoglobulin data, examples 1-3 are overall better than all the comparative formula, and the advantage is more significant as the experimental period is prolonged. The changes of raw sheep milk, whey protein complex and probiotics all affect the content of immunoglobulin, indicating that the combination of each raw material of the present application synergistically plays a more optimal role in improving immunity.

[0081] As can be seen from Table 4, the cytokine data shows that, compared with all the comparative groups, the content of anti-inflammatory factor IL-2 of examples 1-3 is higher, while the content of pro-inflammatory factors IL-6 and TNF-α is lower. The content of IL-2 of comparative examples 6-7 is lower than that of example 1 due to the replacement of probiotic strains, indicating that the combination of Bifidobacterium longum subsp. longum BB536, Fermented Lactobacillus muci lagosus CECT5716 and Lactobacillus helveticus R0052 can more accurately regulate the balance of immune cytokines, reduce inflammatory response and enhance immune defense ability. The comparison between the example group and the comparative example group shows that the changes of raw sheep milk, whey protein complex and probiotics all affect the content of each cytokine, indicating that the combination of each raw material of the present application synergistically plays a more optimal role in improving immunity.

[0082] Example 4 Analysis of low allergenicity effect On the 28th day of the experiment of example 1, the serum of each group of rat pups was collected, and the method was the same as that of serum collection in immunity index detection. The content of histamine and trypsin in serum was detected by ELISA method, and the operation was carried out according to the corresponding kit instruction.

[0083] Table 5 Serum allergen-related substance levels

[0084] As can be seen from Table 5, the serum histamine and trypsin levels of examples 1-3 are overall lower than those of comparative examples 1-7, and the two indicators of example 3 are the lowest. Comparative examples 2-3 do not use the original formula of whey protein ultrasonic enzymolysis process, and the allergen index is significantly deteriorated, which proves that this process can decompose macromolecular proteins into small molecular peptides, reducing the probability of immune recognition and sensitization.

[0085] Example 5 Detection of digestion function related indicators On the 28th day of the experiment of example 1, 10 rats of each group were fasted for 12h, then executed by decapitation, quickly dissected, and the stomach and small intestine were taken out, washed with normal saline, and then dried with filter paper. At the same time, the feces of rat pups in the past 24h were collected, weighed and stored in a refrigerator at -20℃.

[0086] Pepsin and trypsin activity detection: 0.5 g of gastric tissue and small intestinal tissue were taken, added with physiological saline homogenate, centrifuged at 3000 r / min for 15 min, and the supernatant was taken. The pepsin activity was detected by the Folin-phenol method, and the trypsin activity was detected by the casein hydrolysis method. The specific operation was carried out according to the related reagent kit instruction.

[0087] Fecal water content and pH value detection: 1 g of fecal sample was weighed, 9 mL of distilled water was added, and it was fully stirred and uniformly mixed. The water content of the feces was detected by using a moisture meter; the pH value of the fecal homogenate was directly detected by using a pH meter.

[0088] Table 6: Detection results of indicators related to digestive function

[0089] As shown in Table 6, the pepsin activity and trypsin activity of Examples 1-3 were higher than those of all the comparative examples. The fecal water content and pH value of Examples 1-3 were closer to the fecal characteristics of breastfed infants, while the fecal water content of Comparative Examples 2-3 was less than 60%, and the pH value was more than 7.0, indicating that the intestinal environment was alkaline, which easily led to incomplete protein digestion. The pH value of the feces of the comparative example was higher than that of the example because the proportion of casein in the raw cow milk was higher, which proved that the protein composition of the sheep milk was more suitable for the acid-base balance requirement of the infant intestinal tract, and further indicated that the milk powder was closer to breast milk.

[0090] The above only describes the preferred embodiments of the present application, and it should be noted that for ordinary skilled persons in the art, several improvements and refinements can be made without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. An infant formula that mimics human milk, characterized in that, The infant formula milk powder comprises the following raw materials by weight: raw goat milk 55-65 parts, whey protein complex 5-15 parts, compound oil 1-4 parts, compound vitamins 0.03-0.2 parts, compound minerals 0.03-0.2 parts, prebiotics 3-6 parts, probiotics 0.06-0.2 parts, choline chloride 0.005-0.015 parts, taurine 0.003-0.01 parts, nucleotides 0.004-0.01 parts, docosahexaenoic acid 0.05-0.2 parts, and arachidonic acid 0.05-0.2 parts; The whey protein complex is prepared from the following raw materials: α-lactalbumin, β-lactoglobulin, lactoferrin, and immunoglobulin, and the mass ratio of the above-mentioned raw materials is (2.8-3.2):(1.8-2.2):(0.08-0.12):(0.04-0.06), respectively.

2. The infant formula of claim 1, wherein, The compound oil comprises soybean oil, olive oil, coconut oil, and medium-chain fatty acid MCT, and the mass ratio of the above-mentioned raw materials is (6.5-8.5):(3.5-5.5):(1.5-3.5):(1-3), respectively.

3. The infant formula of claim 1, wherein, The probiotics comprise Bifidobacterium longum subsp. longum BB536, Lactobacillus fermentum CECT5716, and Lactobacillus helveticus R0052, and the mass ratio of the above-mentioned raw materials is (0.5-1.5):(1.5-2.5):(0.5-1.5), respectively.

4. The infant formula of claim 1, wherein, The prebiotics comprise 2'-fucosyllactose, galacto-oligosaccharide, and lactulose, and the mass ratio of the three is (0.5-1.5):(2.5-3.5):(2.5-3.5), respectively.

5. The process for the preparation of an infant formula as claimed in any of claims 1 to 4, characterized in that, The method comprises the following steps: mixing and enzymatically hydrolyzing a whey protein complex raw material solution with a compound protease to obtain a whey protein complex; uniformly mixing raw goat milk, the whey protein complex, compound oil, compound vitamins, compound minerals, choline chloride, and taurine, adjusting the pH, and then homogenizing, sterilizing, and concentrating to obtain a milk powder; and uniformly mixing the milk powder with prebiotics, probiotics, docosahexaenoic acid, arachidonic acid, and nucleotides.

6. The preparation method according to claim 5, characterized in that, The compound protease comprises trypsin and papain, and the addition amount of the compound protease is 0.2-0.6 wt% of the total mass of the whey protein complex raw material solution.

7. The preparation method according to claim 6, characterized in that, The mass ratio of the trypsin to the papain is (1-3):(0.5-1.5), the enzyme activity of the trypsin is 3500-4500 U / g, and the enzyme activity of the papain is 90-110 thousand U / g.

8. The preparation method according to claim 5, characterized in that, The pH is 6.8-7.

2.

9. The preparation method according to claim 5, characterized in that, The homogenization conditions comprise 25-35 MPa, 35-45℃, and 1-4 times.

10. Use of the infant formula milk powder of any one of claims 1-4 or the infant formula milk powder obtained by the preparation method of any one of claims 5-9 in the preparation of an immune-enhancing and hypoallergenic product.

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