Application of raspberry tea extract in increasing content of coenzyme Q10 in blood
By promoting coenzyme Q10 synthesis through berry tea extract, the problem of decreased coenzyme Q10 levels in the blood caused by high-fat diets and statin drugs was solved, and the coenzyme Q10 levels in rat blood were significantly increased. It has antioxidant, blood sugar and lipid regulation, and hangover relief and liver protection effects.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-03
- Publication Date
- 2026-03-10
AI Technical Summary
In the current technology, high-fat diets and statin drugs can lead to a decrease in the level of coenzyme Q10 in the blood. A lack of coenzyme Q10 may cause various cardiovascular and cerebrovascular diseases and skin aging. The application of berry tea extract has not yet been reported.
By applying berry tea extract to rats, the levels of coenzyme Q10 in the blood of rats fed a high-fat diet and administered simvastatin via gavage were significantly increased, indicating that berry tea extract promotes the synthesis of coenzyme Q10.
Berry tea extract significantly increased the level of coenzyme Q10 in rat blood, restoring or surpassing the decrease caused by a high-fat diet and statins, and has significant antioxidant, blood sugar and lipid regulation, and hangover relief and liver protection effects.
Smart Images
Figure CN121622798A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of health food technology, and in particular to the preparation of berry tea extract to increase coenzyme Q in the blood. 10 Application in products with high content. Background Technology
[0002] Coenzyme Q 10 (CoQ 10 Coenzyme Q10 is a naturally occurring compound found in the human body, present in every cell. 10 It has antioxidant properties, helps maintain heart health, provides energy, boosts the immune system, and maintains healthy skin. Coenzyme Q10 10 It can be used as an adjunct treatment for cardiovascular diseases, providing nutrients to myocardial cells and enhancing metabolic rate, thus having a certain auxiliary effect on cardiovascular and cerebrovascular diseases. In addition, Coenzyme Q... 10 It participates in the cell's energy metabolism process, promotes the production of ATP, provides the energy needed by the cell, and increases the body's vitality and endurance.
[0003] Coenzyme Q 10 A deficiency in coenzyme Q can lead to various diseases; the human body lacks coenzyme Q. 10 This can easily lead to insufficient blood supply to the brain and heart, resulting in fatigue, poor heart function, memory loss, dark circles under the eyes, dull skin, and weakened immunity. If coenzyme Q is not supplemented in time... 10 It is more likely to cause various cardiovascular and cerebrovascular diseases such as heart failure, arrhythmia, myocardial infarction, angina pectoris, coronary heart disease, stroke, hypertension, hyperlipidemia, and arteriosclerosis. At the same time, the skin ages faster, thus accelerating aging.
[0004] Unhealthy lifestyle habits, such as consuming a high-fat, high-sugar diet, may affect the body's coenzyme Q10 levels. 10 The reason is that a high-fat diet may increase oxidative stress, leading to a decrease in coenzyme Q10 levels. 10 Increased consumption; while a high-sugar diet may affect coenzyme Q. 10 The synthesis and stability of coenzyme Q are affected, thus reducing its intracellular levels. Furthermore, adverse reactions to some drugs can also lead to decreased levels of coenzyme Q. 10 A decrease in levels, such as with statins, is caused by coenzyme Q10. 10 Coenzyme Q10 biosynthesis occurs in the mitochondrial endoplasmic reticulum. An important enzyme in this pathway is HMG-CoA reductase, and statins reduce coenzyme Q10 levels by inhibiting HMG-CoA reductase activity. 10 The synthesis of [something] causes myopathy and rhabdomyolysis.
[0005] Wild vine tea, also known as small-leaf vine tea, longevity vine, and Maoyan wild vine tea, is a precious wild vine plant tea. Wild vine tea possesses various protective effects, including anti-inflammatory, antioxidant, blood sugar and lipid-regulating, and hangover-relieving properties. It mainly grows in southern China, such as Zhangjiajie in Hunan Province, and is a natural edible resource. Although wild vine tea has unique health benefits and rich nutritional value, it has not been fully developed and utilized, especially regarding the effects of wild vine tea extract on coenzyme Q10 levels in the blood. 10 The impact of content has not yet been reported. Summary of the Invention
[0006] This invention provides the application of berry tea extract in increasing the level of coenzyme Q10 in the blood. This invention studies the effect of berry tea extract on the level of coenzyme Q10 in the blood of rats. 10 The effect of content: When berry tea extract was applied to rats on a long-term high-fat diet, it significantly increased the level of coenzyme Q in the rat blood. 10 Content. Simultaneously, when berry tea extract was administered to rats long-term via gavage with simvastatin, it significantly increased the level of coenzyme Q in the rat blood. 10 content.
[0007] Specifically, this is achieved through the following technical solutions:
[0008] Firstly, this application provides information on the preparation of berry tea extract to enhance blood coenzyme Q. 10 Application in products with high content.
[0009] In some embodiments, the product is a health food, a pharmaceutical, or a laboratory preparation.
[0010] In some embodiments, the dosage form of the product includes any one of tablets, capsules, powders, granules, oral preparations, intravenous injections, or gels.
[0011] Secondly, this application provides a method for increasing coenzyme Q in the blood. 10 The product contains a certain amount of berry tea extract.
[0012] Thirdly, this application provides a method for increasing blood coenzyme Q not for the purpose of disease diagnosis and treatment. 10 The method involves administering berry tea extract or the aforementioned products to the test subjects.
[0013] In some embodiments, the amount of the berry tea extract used is 1.0-40 mg / kg.
[0014] Compared with the prior art, the advantages of the present invention are:
[0015] This invention demonstrates that by applying berry tea extract to rats on a long-term high-fat diet, the berry tea extract can promote the production of coenzyme Q. 10The synthesis of [the substance] significantly increased the level of coenzyme Q in rat blood. 10 Content. Simultaneously, when berry tea extract was administered to rats long-term via gavage with simvastatin, the berry tea extract was able to promote coenzyme Q. 10 The synthesis of [the substance] significantly increased the level of coenzyme Q in rat blood. 10 Content. Therefore, berry tea extract can be used to prepare products that enhance coenzyme Q. 10 Products with high content. Attached Figure Description
[0016] Figure 1 Coenzyme Q in rat blood in Example 3 10 Comparison chart of content;
[0017] Figure 2 Coenzyme Q in rat blood in Example 4 10 A comparison chart of content. Detailed Implementation
[0018] The technical solution of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0019] In the following examples, the high-fat diet was purchased from Jiangsu Xiehe Pharmaceutical Biotechnology Co., Ltd.; coenzyme Q in rat blood... 10 The content was determined using high performance liquid chromatography-mass spectrometry (HPLC-MS / MS).
[0020] Example 1: Preparation of Berry Tea Extract
[0021] Weigh 5g of berry tea raw material into a 250ml round-bottom flask, add 250ml of double-distilled water, and extract at 100℃ for 1.5h, then start stirring. Repeat the extraction twice, filtering while hot using a Buchner funnel. Combine the two extracts and rotary evaporate under reduced pressure at 70℃ to obtain an extract density of approximately 1.05g / mL. Pour out the extract and pre-freeze in a refrigerator, then freeze-dry in a freeze dryer. After freeze-drying, collect the powder, weigh it, and obtain 2.47g of berry tea extract.
[0022] Example 2: Preparation of Berry Tea Extract
[0023] Weigh 100g of berry tea raw material into a 1000ml round-bottom flask, add 500ml of double-distilled water, and extract at 50℃ for 1.5h, then start stirring. Repeat the extraction twice using the same method, filtering while hot using a Buchner funnel. Combine the two extracts and rotary evaporate under reduced pressure at 70℃ to obtain an extract density of approximately 1.15g / mL. Pour out the extract and pre-freeze in a refrigerator, then freeze-dry in a freeze dryer. After freeze-drying, collect the powder, weigh it, and obtain 40.28g of berry tea extract.
[0024] Experimental Example 1: Effects of Berry Tea Extract on Coenzyme Q in the Blood of Rats Dieted on a High-Fat Diet for an Extended Period 10 Effect of content
[0025] 1. Test Methods
[0026] 1.1 Twenty-four 6-week-old SD rats were purchased and randomly divided into three groups according to their body weight: blank group, high-fat diet group, and berry tea extract group, with eight rats in each group.
[0027] 1.2 The rats were housed in IVC (Independent Ventilation and Ventilation) cages with independent ventilation. During the entire rearing period, the rats' water and food were changed every two days, and the bedding was changed twice a week to keep the cages clean. The temperature in the rearing room was maintained at 20-25℃ and the humidity was maintained at 50-60%.
[0028] 1.3 After one week of acclimatization feeding, rat modeling was initiated. The control group received no treatment; the high-fat diet group received a high-fat diet daily; and the berry tea extract group received a high-fat diet daily, along with daily gavage administration of the berry tea extract prepared in Example 2 at a dose of 250 mg / kg (equivalent to 40 mg / kg in humans). After three months of this treatment, the levels of coenzyme Q in the blood of rats in each group were measured. 10 The content was determined, and the test results are as follows: Figure 1 As shown.
[0029] 2. Analysis of Experimental Results
[0030] Depend on Figure 1 It can be seen that, compared with the control group, the high-fat diet group had higher levels of coenzyme Q in the blood of rats. 10 The level was significantly reduced. Compared to before, the level of coenzyme Q in rat blood was significantly lower. 10 It decreased by 50.55%. This is because a high-fat diet increases oxidative stress, leading to a decrease in coenzyme Q10 levels. 10 Increased consumption of coenzyme Q10 leads to higher levels of coenzyme Q10 in rat blood. 10 The content was significantly reduced.
[0031] Compared with the high-fat diet group, the rats in the berry tea extract group had higher levels of coenzyme Q in their blood. 10 The level increased significantly. Compared to before, the level of coenzyme Q in rat blood was significantly higher.10 It increased by 2.4 times. This is because after gavage administration of berry tea extract, the extract can promote the production of coenzyme Q10. 10 The synthesis of [the substance] significantly increased the level of coenzyme Q in rat blood. 10 content.
[0032] Experimental Example 2: Effects of Berry Tea Extract on Coenzyme Q in the Blood of Rats Long-Term Oral Administration of Simvastatin 10 Effect of content
[0033] 1. Test Methods
[0034] 1.1 Twenty-four 6-week-old SD rats were purchased and randomly divided into three groups according to body weight: blank group, simvastatin group, and berry tea extract group, with eight rats in each group.
[0035] 1.2 The rats were housed in IVC (Independent Ventilation and Ventilation) cages with independent ventilation. During the entire rearing period, the rats' water and food were changed every two days, and the bedding was changed twice a week to keep the cages clean. The temperature in the rearing room was maintained at 20-25℃ and the humidity was maintained at 50-60%.
[0036] 1.3 After one week of acclimatization feeding, rats were used to establish a model. The control group received no treatment; the simvastatin group received simvastatin (10 mg / kg) by gavage daily; and the berry tea extract group received simvastatin (10 mg / kg) by gavage daily, along with the berry tea extract prepared in Example 1 at a dose of 6.25 mg / kg (equivalent to 1 mg / kg in humans). After three months of this treatment, the levels of coenzyme Q in the blood of rats in each group were measured. 10 The content was determined, and the test results are as follows: Figure 2 As shown.
[0037] 2. Analysis of Experimental Results
[0038] Depend on Figure 2 It can be seen that, compared with the blank group, the simvastatin group had higher levels of coenzyme Q in the blood of rats. 10 The content was significantly reduced; compared with before and after, the level of coenzyme Q in rat blood was significantly lower. 10 It decreased by 69.2%. The reason is coenzyme Q. 10 The biosynthesis of coenzyme Q10 occurs in the mitochondrial endoplasmic reticulum. An important enzyme in this pathway is HMG-CoA reductase. Simvastatin inhibits the activity of HMG-CoA reductase, thereby reducing the amount of coenzyme Q10. 10 The synthesis of coenzyme Q in rat blood leads to its higher levels. 10 The content was significantly reduced.
[0039] Compared with the simvastatin group, the rats in the berry tea extract group had lower levels of coenzyme Q in their blood. 10The level increased significantly. Compared to before, the level of coenzyme Q in rat blood was significantly higher. 10 It increased by 4.07 times. This is because after gavage administration of berry tea extract, the extract can promote the production of coenzyme Q10. 10 The synthesis of [the substance] significantly increased the level of coenzyme Q in rat blood. 10 content.
[0040] The above detailed embodiments describe the implementation of the present invention; however, the present invention is not limited to the specific details described in the above embodiments. Within the scope of the claims and technical concept of the present invention, various simple modifications and changes can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.
Claims
1. A type of berry tea extract for preparing blood coenzyme Q2. 10 Application in products with high content.
2. Use of the berry tea extract according to claim 1 for the manufacture of a product for raising the coenzyme Q 10 content in blood, characterized in that, The product is a health food, a medicine or a laboratory preparation.
3. Use of the berry tea extract according to claim 1 in the manufacture of a product for raising the level of coenzyme Q 10 in the blood, characterized in that The dosage form of the product includes any one of a tablet, a capsule, a powder, a granule, an oral agent, an intravenous injection, an effervescent agent, a candy, a pill or a gel.
4. A product for raising the level of coenzyme Q in blood, characterized in that, 10 The product includes a Rubus suavissimus extract. 5. A method of increasing the level of coenzyme Q in blood not for the purpose of diagnosis and treatment of diseases, characterized by, 10 The subject is administered the Rubus suavissimus extract or the product of claim 4. 6. The method of increasing the content of coenzyme Q in blood not for the purpose of disease diagnosis and treatment according to claim 5, characterized by, 10 The Rubus suavissimus extract is administered in an amount of 1.0-40 mg / kg.