Essential oil pellet for resisting stress of cats as well as preparation method and application of essential oil pellet

Abstract

The invention discloses an essential oil pellet for resisting stress of cats as well as a preparation method and application of the essential oil pellet, and belongs to the technical field of veterinary traditional Chinese medicine preparations. The essential oil pellet is prepared from the following raw materials: extracts of wormwood, lavender and rhizoma acori graminei; the mass ratio of wormwood to lavender to rhizoma acori graminei is (1-2): (1-2): (1-2). The essential oil pellet prepared by the invention is filled into a nylon bag to prepare a sachet, and the sachet is suspended on the neck of a cat, added into a cat collar or put into cat clothes for use, so that the anti-stress capability of the cat can be remarkably improved, specifically, the feed intake of the cat is improved, and the weight of the cat is increased; meanwhile, the antioxidant index is improved, and the level of oxidative metabolite MDA in blood is reduced; in addition, the levels of cortisol and aldosterone as stress hormones and interleukin-6 as an inflammatory cytokine in cat serum can also be reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of veterinary traditional Chinese medicine preparations, in particular to a kind of essential oil micro-pellets for cat anti-stress and preparation method and application thereof. BACKGROUND

[0002] In recent years, China's rapid economic development, people's living standards have improved significantly, as a companion animal pets have entered thousands of households, raising cats has become a hot, more and more by young people, pet cat health is increasingly concerned by people. However, a large number of stress stimuli in the environment pose a serious threat to the health of cats. Stress stimuli encountered in daily life of pet cats mainly include hunger, cold and heat, transportation, contact with companion animals, noise, vaccine injection, cage change, allergic reaction, hormone changes during pregnancy of female cats, fear and fright, environmental mutation, owner's scolding and cold, etc. Stress can cause many behavioral changes in cats, such as decreased food intake, reduced grooming behavior, quiet and lying, reduced play behavior, reduced exploration behavior, reduced interaction with other cats and humans, increased vigilance, increased urine marking and aggressive behavior, etc. When cats are subjected to severe, sudden, long-term or chronic stress, they may even induce diseases such as severe gastrointestinal reactions (vomiting, diarrhea, anorexia, pica, etc.), induce depression, sensory hypersensitivity syndrome, and psychogenic alopecia; some cause serious urinary system diseases such as urinary incontinence, frequent urination, and color change, which may exacerbate the symptoms of cat spontaneous cystitis; the most serious part of the cats suffers from immune system suppression diseases, and even causes sudden death. SUMMARY

[0003] The present application aims to solve the above problems in the prior art, and provides essential oil micro-pellets for cat anti-stress and preparation method and application thereof.

[0004] The technical solution adopted by the present application is as follows:

[0005] An essential oil micro-pellet for cat anti-stress, the essential oil micro-pellet is composed of the following raw materials: extracts of wormwood, lavender and rhizoma alocasia; the mass ratio of the wormwood, lavender and rhizoma alocasia is 1-2:1-2:1-2.

[0006] Preferably, the mass ratio of the wormwood, lavender and rhizoma alocasia is 2:2:1.

[0007] The present application simultaneously provides a preparation method of the above essential oil micro-pellet, comprising the following steps:

[0008] S1, the raw materials wormwood, lavender and rhizoma alocasia are weighed according to the proportion, the plant essential oils of the three raw materials are extracted by steam distillation method and mixed to obtain a mixed essential oil; the mixed essential oil is dissolved in ethanol to obtain an essential oil ethanol solution;

[0009] S2. Add the essential oil ethanol solution from S1 dropwise to a 10% β-cyclodextrin aqueous solution, stir at 40°C for 3 hours; then let stand at 4°C for 12 hours and filter. The filtrate is dried under vacuum to obtain a dry powder.

[0010] S3. Mix the dry powder, microcrystalline cellulose, chitosan powder and talc powder in S2 evenly, add a small amount of water to moisten, stir until a soft wet block is formed, and use an extruder to extrude the wet block through a 2 mm aperture to form columnar particles.

[0011] S4. Roll the columnar particles from S3 into rounded particles using a rounding machine at a speed of 800–1200 rpm to obtain microsphere particles;

[0012] S5. Lightly spray EC / HPMC aqueous alcohol solution onto the surface of the microspheres in S4, then roll dry, controlling the coating thickness to 5% of the total weight; dry in an oven at 50℃ to constant weight to obtain anti-stress sustained-release microspheres.

[0013] Preferably, the volume ratio of the mixed essential oil to ethanol in S1 is 1:3.

[0014] Preferably, in S3, by mass percentage, there are 80% dry powder, 10% microcrystalline cellulose, 5% chitosan powder, and 5% talc.

[0015] Preferably, the particle size of the microspheres in S4 is 1–2 mm.

[0016] The present invention also provides the application of the above-mentioned essential oil microcapsules in the preparation of cat anti-stress products, wherein the essential oil microcapsules are put into nylon bags to make sachets, which are then hung around the cat's neck, added to the cat's collar, or placed in the cat's clothing for use.

[0017] The beneficial effects of this invention are as follows:

[0018] 1. The essential oil microcapsules prepared by this invention greatly enhance the stability of the essential oil compared to the original medicinal materials, resulting in a more stable and longer-lasting release.

[0019] 2. The essential oil microcapsules prepared in this invention are packaged into nylon bags to make sachets. These sachets can be hung around a cat's neck, added to a cat collar, or placed in a cat's clothing. They can significantly improve a cat's stress resistance, specifically by increasing the cat's food intake, thereby increasing the cat's weight; increasing antioxidant indicators and reducing the level of the oxidative metabolite MDA in the blood; and also reducing the levels of the stress hormones cortisol and aldosterone and the inflammatory cytokine interleukin-6 in the cat's serum. Attached Figure Description

[0020] Figure 1 Release curve of the anti-stress sachet. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0022] Example 1

[0023] Preparation of cat anti-stress essential oil microcapsule sachets

[0024] 1. First, the essential oils of Artemisia argyi, lavender and Acorus calamus were extracted by steam distillation with a mass ratio of 2:2:1. The mixed essential oils were dissolved in ethanol (volume ratio 1:3).

[0025] 2. Add essential oil ethanol solution dropwise to 10% β-cyclodextrin aqueous solution (β-CD), stir at 40°C for 3 hours; then place the sample in a refrigerator (4°C) and let it stand for 12 hours before filtering. The filtrate is then vacuum dried to obtain dry powder (β-CD@essential oil inclusion complex).

[0026] 3. Weigh out 80% of β-CD essential oil inclusion complex, 10% of microcrystalline cellulose (MCC), 5% of chitosan powder, and 5% of talc powder according to the proportions, mix them evenly, add a small amount of water to moisten, stir until a soft wet block is formed, and use an extruder to extrude the wet block through a 2 mm aperture to form columnar particles.

[0027] 4. Then roll the microspheres with a rolling mill at a speed of 800–1200 rpm to obtain approximately spherical microspheres (particle size controlled at 1–2 mm).

[0028] 5. After rounding, lightly spray EC / HPMC water-alcohol solution, then roll and dry, controlling the coating thickness to 5% of the total weight; dry in an oven at 50℃ to constant weight to obtain anti-stress sustained-release essential oil microcapsules.

[0029] 6. You can put essential oil microcapsules into a nylon bag to make a sachet and hang it around your pet's neck, or add it to a collar, or put it in your pet's clothing to achieve a similar effect.

[0030] Example 2

[0031] Efficacy test of cat anti-stress microcapsules

[0032] 1. Experimental Methods

[0033] Environmental change is one of the main factors causing stress in cats. An experiment was conducted at a cattery in Chengdu, primarily using British Shorthair cats. The sold cats were divided into three groups (control group 1, control group 2, and sachet group), with 10 cats in each group. Control group 1: empty nylon bags were hung; Control group 2: nylon bags containing powdered herbs (artemisia, lavender, and calamus in a 2:2:1 mass ratio) were hung; Sachet group: micro-pellet sachets prepared in Example 1 were hung. Starting from the day of arrival, the cats' food intake was recorded and analyzed daily, and blood samples were collected on the 10th day to test stress-related indicators.

[0034] 2. Effects on cats' food intake, water consumption, and basic physiological indicators

[0035] As shown in Table 1, environmental stress significantly reduced cats' food intake, which in turn affected their weight gain. The anti-stress sachet significantly improved cats' food intake on day 10, and its effect was better than that of the control group.

[0036] Table 1. Cat's food intake, water consumption, and basic physiological indicators

[0037]

[0038] 3. Effects on antioxidant levels in cats

[0039] Table 2 shows that environmental stress still significantly affected the levels of antioxidant indicators SOD and GSH-Px on day 10. The antioxidant indicators of the anti-stress sachet group were significantly higher than those of the control group 2. At the same time, the anti-stress sachet also significantly reduced the level of the oxidative metabolite MDA in the blood. Stars indicate significant differences compared with the control group 2.

[0040] Table 2. Detection of antioxidant levels in cats

[0041]

[0042] 4. Effects on cat stress hormones

[0043] Table 3 shows that the anti-stress sachet significantly reduced the levels of stress hormones cortisol and aldosterone in cat serum on day 10, while also reducing the level of the inflammatory cytokine interleukin-6. Starred items indicate significant differences compared to control group 2.

[0044] Table 3. Cat Stress Hormone Level Detection

[0045]

[0046] Example 3

[0047] The preparation method of the cat anti-stress essential oil microcapsule sachet in this embodiment is the same as that in Example 1, except that the ratio of mugwort, lavender and calamus in this embodiment is 2:1:1.

[0048] Example 4

[0049] The preparation method of the cat anti-stress essential oil microcapsule sachet in this embodiment is the same as that in Example 1, except that the ratio of mugwort, lavender and calamus in this embodiment is 1:2:1.

[0050] Example 5

[0051] The preparation method of the cat anti-stress essential oil microcapsule sachet in this embodiment is the same as that in Example 1, except that the ratio of mugwort, lavender and calamus in this embodiment is 1:2:2.

[0052] Example 6

[0053] The preparation method of the cat anti-stress essential oil microcapsule sachet in this embodiment is the same as that in Example 1, except that the ratio of mugwort, lavender and calamus in this embodiment is 2:1:2.

[0054] The anti-stress effects of the essential oil microcapsule sachets prepared in Examples 3-6 on cats were tested according to the method in Example 2. The control groups in Examples 3-6 were, in turn, powders of Artemisia argyi, lavender, and Acorus calamus in mass ratios of 2:1:1, 1:2:1, 1:2:2, and 2:1:2, respectively. The test results are shown in Table 4.

[0055] Table 4. Detection data of different ratios of traditional Chinese medicine for cat anti-stress

[0056]

[0057] Example 7

[0058] Detection of the release time of the main aromatic substances in cat anti-stress microcapsules

[0059] A 1L sealed, dry glass sampler with ventilation was used, with the air inlet at the bottom and the air outlet at the top, serving as the sampling port. 1g of anti-stress sustained-release microcapsules (prepared in Example 1) were placed in a nylon bag and placed inside the sampler. During sampling, 2L of air was introduced into the sampler through the air inlet and passed through an activated carbon adsorption tube. After pyrolysis adsorption, the adsorption tube was loaded onto a gas chromatograph for detection. Using eucalyptol in Artemisia argyi as a statistical index, the release of anti-stress sustained-release microcapsules at 1, 2, 3, and 4 weeks was statistically analyzed and compared with unextracted, proportionally proportioned powder of the medicinal material. The results are as follows: Figure 1 As shown in the results, the anti-stress sachet significantly enhances the stability of the essential oils compared to the original medicinal materials, resulting in a more stable and longer-lasting release.

[0060] The specification and drawings of this invention are intended to be illustrative rather than restrictive. Based on this invention, those skilled in the art can make substitutions and modifications to some of the technical features without creative effort, and all such modifications are within the scope of protection of this invention.

Claims

1. An essential oil micro-pellet for feline anti-stress, characterized in that, The essential oil micro-pellets are composed of extracts of wormwood, lavender and Acorus calamus; the mass ratio of the wormwood, lavender and Acorus calamus is 1-2:1-2:1-2.

2. The micro-pellets of essential oils according to claim 1, characterized in that, The mass ratio of the wormwood, lavender and Acorus calamus is 2:2:

1.

3. The method for preparing essential oil microcapsules as described in claim 1 or 2, characterized in that, The method comprises the following steps: S1, the raw materials of wormwood, lavender and Acorus calamus are weighed according to the proportion, and the essential oils of the three raw materials are extracted by steam distillation and mixed to obtain a mixed essential oil; the mixed essential oil is dissolved in ethanol to obtain an essential oil ethanol solution; S2, the essential oil ethanol solution in S1 is added dropwise to a 10% β-cyclodextrin aqueous solution, stirred at 40°C for 3 hours, then placed at 4°C for 12 hours, filtered, and the filtrate is dried under vacuum to obtain a dry powder; S3, the dry powder in S2, microcrystalline cellulose, chitosan powder and talc powder are mixed uniformly, a small amount of water is added to wet them, stirred until soft lumps are formed, and the lumps are extruded through a 2 mm aperture extruder to form columnar particles; S4, the columnar particles in S3 are rolled by a rolling machine at a speed of 800-1200 rpm to obtain microspheres; S5, the surface of the microspheres in S4 is lightly sprayed with an EC / HPMC water-alcohol solution, and then rolled and dried, the coating thickness is controlled at 5% of the total weight; the oven is dried at 50°C until the weight is constant to obtain anti-stress sustained-release micro-pellets.

4. The production method according to claim 3, characterized by, The volume ratio of the mixed essential oil to ethanol in S1 is 1:

3.

5. The preparation method according to claim 3, characterized in that, In S3, the dry powder is 80%, the microcrystalline cellulose is 10%, the chitosan powder is 5%, and the talc powder is 5% by mass percentage.

6. The preparation method according to claim 3, characterized in that, The particle size of the microspheres in S4 is 1-2 mm.

7. Use of the micro-pellets of essential oils according to claim 1 or 2 for the preparation of a cat anti-stress product, characterized in that, The essential oil micro-pellets are packed in nylon bags to make sachets, which are hung on the cat's neck, added to the cat's collar or put into the cat's clothes for use.

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