Method for detecting environmental estrogen

By using the ELRBA kit containing estradiol derivative-bovine serum albumin conjugate and recombinant human estrogen receptor GST-ERα-LBD, the pollution risk associated with radiolabeling has been eliminated, enabling high-precision, rapid, and safe quantitative detection of environmental estrogens.

CN121633495APending Publication Date: 2026-03-10ZHENGZHOU UNIV +1
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Patent Information

Application Number
CN202511889255.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-15
Publication Date
2026-03-10

AI Technical Summary

Technical Problem

Existing methods for detecting environmental estrogens mostly rely on radiolabeled ligands, which pose a risk of radioactive contamination. Furthermore, traditional methods are difficult to accurately quantify all substances with estrogenic effects.

Method used

Using estradiol derivative-bovine serum albumin conjugate as the recognition molecule, combined with recombinant human estrogen receptor GST-ERα-LBD and enzyme-labeled secondary antibody, an indirect competitive reaction was carried out using an ELRBA kit to achieve the detection of estrogen in environmental samples.

Benefits of technology

It achieves high-precision, rapid, and safe quantitative detection of environmental estrogens, avoids the pollution problems caused by radioactive labeling, and has high specificity and sensitivity in the detection process.

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Abstract

The invention relates to the technical field of environmental monitoring and analysis, in particular to a detection method of environmental estrogen. According to the detection method, a recombinant human estrogen receptor GST-ER alpha-LBD is used as a biological recognition molecule, and an anti-GST antibody and a corresponding enzyme-labeled secondary antibody are used as reporter molecules; an estradiol derivative-bovine serum albumin conjugate with a proper connecting arm is coated on a microwell plate, and the equivalent concentration of environmental estrogen is detected in an indirect competition mode. The method for detecting the equivalent concentration of the environmental estrogen is accurate, reliable, sensitive, rapid, green and safe, the detection limit of the estradiol can be as low as 0.072 microgram / L, and the equivalent concentration of the environmental estrogen can be detected.
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