Boschniakia rossica planting method

Abstract

The invention relates to the technical field of boschniakia rossica planting, and particularly discloses a boschniakia rossica planting method which comprises the following steps: S1, seed photochemical synergistic germination induction, S2, artificial parasitic body combination, S3, parasitic body seedling hardening and seedling strengthening, S4, site selection and transplantation, S5, growth microenvironment intelligent regulation and control, S6, staged precise nutrition supply and S7, target harvesting and regeneration management. According to the boschniakia rossica planting method provided by the invention, a parasitic establishment process between the boschniakia rossica and the host alder is transferred from an uncontrollable field environment to a highly controllable artificial environment, so that the predictability, the stability and the efficiency of planting are fundamentally improved.

Description

Technical Field

[0001] This invention relates to the field of Cistanche deserticola cultivation technology, and more specifically to a method for cultivating Cistanche deserticola. Background Technology

[0002] Cistanche deserticola, a rare medicinal plant of the Orobanchaceae family, has attracted much attention due to its unique medicinal value. However, its obligate root parasitic biological characteristics, namely that its seeds must depend on a host plant, primarily alder, and that specific signals emitted by the alder root system are required for germination and successful establishment of a parasitic connection to obtain nutrients, constitute a fundamental bottleneck in its resource development and utilization.

[0003] Currently, the propagation of Cistanche deserticola mainly relies on the cultivation of wild resources and limited biomimetic cultivation. Existing technical approaches generally focus on intervening in the seeds and host through physical or chemical methods in natural or semi-natural habitats to promote parasitism under wild conditions. Conventional practices include collecting mature seeds and then directly broadcasting or sowing them in rows in wild alder forests, supplemented by management measures such as root pruning and mulching. These methods attempt to mimic and optimize the natural reproductive process of Cistanche deserticola in complex natural environments; however, this technical approach, which heavily relies on natural habitats, has several inherent drawbacks that are difficult to overcome.

[0004] First, the germination of Cistanche deserticola seeds requires the reception of specific chemical signals from the host root system, as well as the synergistic effect of multiple factors such as suitable temperature, humidity, and soil microbial community. This process is highly uncertain and uncontrollable in the wild environment, resulting in extremely low and unstable seed germination rates.

[0005] Secondly, even if the seeds germinate, it is highly unlikely that their tiny radicles can successfully locate and attach to the host root system in the vast soil space, thereby forming an effective parasitic haustorium. This makes it difficult to guarantee the overall parasitic success rate and results in huge fluctuations in yield.

[0006] Furthermore, existing methods typically require the host tree, alder, to reach a certain age and have a high degree of canopy closure. This not only limits the selection of suitable planting areas, but the long production cycle also delays the realization of industrial benefits. From a production model perspective, the aforementioned technologies are difficult to standardize, and the production process is significantly constrained by climatic conditions, failing to meet the industrial demands for large-scale and stable production, resulting in a long-standing supply-demand imbalance for Cistanche deserticola.

[0007] Therefore, how to provide a Cistanche deserticola cultivation method that improves the predictability, stability and efficiency of cultivation is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0008] In view of this, the present invention provides a method for cultivating Cistanche deserticola, which transfers the parasitic establishment process between Cistanche deserticola and its host Aldera stenoptera from an uncontrollable wild environment to a highly controllable artificial environment, thereby fundamentally improving the predictability, stability and efficiency of cultivation.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: A method for cultivating Cistanche deserticola includes the following steps: S1. Seed photo-chemical synergistic germination induction: Mature Cistanche deserticola seeds were collected, surface-sterilized, and placed in a non-sterile extract culture medium of Alder root solidified with agarose, and cultured in a light incubator. S2. Artificial Parasite Integration: The Cistanche deserticola seeds that successfully germinated and elongated their radicles in step S1 were transferred to a solid symbiotic culture medium. The solid symbiotic culture medium was covered with an ultrafine fiber mesh that simulated the surface structure of the root system. At the same time, the roots of the pre-cultured aseptic tissue culture seedlings of Aldera sibiricum were placed in the fiber mesh area, so that the radicles of Cistanche deserticola and the roots of Aldera sibiricum could come into natural contact in three-dimensional space. By controlling the carbon source and auxin concentration in the culture medium, the tips of the radicles of Cistanche deserticola were specifically adsorbed onto the roots of Aldera sibiricum and formed an artificial parasite. S3. Parasite hardening and seedling strengthening: The artificial parasites with stable parasitic connections from step S2 are transferred from the sterile environment to the seedling trays containing a light moisture-retaining substrate and cultivated in an environmental hardening room. By gradually reducing the air humidity and increasing the light intensity in a programmed manner, the parasites are adapted to the non-sterile environment, and their root development and plant vigor are promoted. S4. Site selection and transplanting: At the selected planting site, holes are drilled in the roots of the host alder according to the size of the artificial parasite's root system. The holes are backfilled with a planting substrate made of local soil, organic matter and water-retaining agent in a certain proportion. The healthy parasite seedlings are transplanted into the holes with their clumps intact, ensuring that their original parasitic connection structure and root system are intact. S5. Intelligent regulation of growth microenvironment: A shade net system with adjustable height and shading rate is built above the transplanting area. At the same time, soil temperature and humidity sensors and drip irrigation network are installed. Based on the sensor data feedback, irrigation and shading are automatically adjusted to create and maintain a relatively stable root zone and canopy microenvironment with light, temperature and humidity for the growth of Cistanche deserticola. S6. Precise staged nutrient supplementation: Based on the phenological stage of Cistanche deserticola's transformation from vegetative growth to reproductive growth, water-soluble fertilizers with different formulations are applied in a pulsed manner through the drip irrigation system. In the early stage of growth, nitrogen is emphasized to promote biomass accumulation, and in the early stage of flowering, phosphorus and potassium fertilizers and boron and zinc micro-fertilizers are applied to increase the content of medicinal components. S7. Target Harvesting and Regeneration Management: When the inflorescence of Cistanche deserticola has fully elongated but has not yet fully bloomed, use a sharp knife to cut and harvest the above-ground parts, retaining the underground corms and parasitic structures. Immediately after harvesting, spray with a fungicide and apply a root-promoting and shoot-growing fertilizer to nourish the underground parts and stimulate the sprouting of new, robust shoots.

[0010] This invention transforms the traditional wild parasitism process, which relies on uncertain natural conditions, into a highly controllable and standardized process in the laboratory and field through steps such as artificial germination induction, artificial parasitism integration, seedling hardening, transplanting, intelligent environmental control, precise nutrient management, and regeneration harvesting. Ultimately, it achieves high germination rate, high parasitism success rate, high survival rate, controllable growth cycle, consistent medicinal quality, and sustainable land use for Cistanche deserticola cultivation, fundamentally solving the core problems of low parasitism rate, unstable yield, and high susceptibility to natural environmental influences in existing technologies.

[0011] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, the illumination light used in the light incubator is provided by red LEDs and blue LEDs in a photon flux density ratio of 7:3, with 12-16 hours of light per day. Through the synergistic effect of the specific illumination light and the chemical signals of the alder root system, seed germination is efficiently and controllably initiated and the radicle is guided to grow in a directional manner.

[0012] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S1, the non-sterile extract of Alder root is prepared by the following method: take the fibrous roots of healthy one-year-old Alder seedlings, wash them, and mix them with distilled water at a weight-to-volume ratio of 1:5-1:10. After soaking at 4°C for 24-48 hours, filter and take the supernatant. The supernatant contains natural chemical signaling substances secreted by Alder root system, which synergistically regulate germination with the light irradiation in the light incubator.

[0013] Low-temperature water extraction can maximize the acquisition and preservation of the complete set of natural chemical signaling substances secreted by alder roots that can induce the germination of Cistanche deserticola seeds. This avoids the problem of insufficient induction efficiency that may exist with single compounds, and provides a highly efficient and more natural comprehensive induction environment for seed germination, thereby significantly improving the germination rate and uniformity of germination.

[0014] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, the peak wavelength of the red LED in the light incubator is 660±5nm and the peak wavelength of the blue LED is 450±5nm. This combination of light quality can not only effectively promote seed germination, but also guide the Cistanche deserticola radicle to present a healthy morphology.

[0015] Red light at 660nm is the main absorption peak of the phytochrome Prf, which can effectively promote seed germination; blue light at 450nm participates in regulating plant morphogenesis and tropism. When the two are combined in a specific ratio, they not only drive the germination process by synergistically activating the light signaling pathway, but also guide the radicle to elongate healthily and in a directional manner, avoiding radicle malformation or growth stagnation.

[0016] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S2, the ultrafine fiber mesh simulating the root surface structure is a polyester fiber nonwoven fabric with a roughened surface. Its fiber diameter is similar to that of Alder root hairs, ranging from 10 to 20 micrometers, providing physical contact stimulation to the Cistanche deserticola embryonic root and guiding its search and contact path with Alder root system.

[0017] The fiber web simulates the physical environment of alder root hairs in natural soil, providing key physical contact stimulation for the radicle of Cistanche deserticola. This stimulation, together with chemical signals, guides the radicle's searching behavior. The three-dimensional structure provides natural contact and entanglement space for both root systems, avoiding damage that may be caused by forced contact, thus promoting the formation of parasitic haustoria and successful parasitism more naturally and efficiently.

[0018] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S2, the solid symbiotic culture medium is based on 1 / 2 MS medium, with the addition of indolebutyric acid at a concentration of 0.05-0.2 mg / L to promote the expansion of Alder roots on the fiber network, while the sucrose concentration is controlled at 10-15 g / L to maintain an adequate carbon source supply without inhibiting the parasitic recognition process.

[0019] Adding a low concentration of indolebutyric acid effectively stimulates the rapid growth and expansion of alder roots within the fiber network, increasing the probability of contact with the radicle of Cistanche deserticola. Maintaining a low sucrose concentration provides the necessary energy for the initial growth of both species while avoiding the potential inhibitory effect of a high-sugar environment on parasitic recognition signaling pathways.

[0020] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S3, the lightweight moisturizing substrate is composed of coconut coir, vermiculite and biochar in a volume ratio of 6:3:1, with a porosity greater than 60% and a pH value stable between 5.8 and 6.5, providing good structural support and buffer protection for the parasitic root system after it leaves the sterile environment.

[0021] The substrate, formulated with a specific ratio, combines high porosity, excellent water retention, and a stable weakly acidic environment. The addition of biochar further improves the substrate structure and helps adsorb harmful substances. This substrate provides a physical buffer and a water-air coordinated transition environment for the fragile parasites after they are removed from agar support, greatly reducing stress during hardening and improving seedling vigor and transplant survival rate.

[0022] Preferably, in the above-mentioned method for planting Cistanche deserticola, in step S4, the volume ratio of local soil, decomposed organic fertilizer and water-retaining agent in the planting substrate is 7:2:1, wherein the water-retaining agent is polyacrylamide type, which can maintain the soil moisture content in the root zone at 60%-70% of the field water holding capacity for a long time after the first saturation water absorption, effectively coping with short-term drought stress.

[0023] The planting substrate utilizes local soil to ensure environmental adaptability, while well-rotted organic fertilizer provides slow-release nutrients. After irrigation, it absorbs and locks in a large amount of water, slowly releasing it to the roots during drought. This characteristic allows the soil moisture in the root zone to be stably maintained within the optimal range for Cistanche deserticola growth, significantly reducing growth stress caused by water fluctuations. It is particularly suitable for mountainous or sloping areas where frequent irrigation is not possible, ensuring stable plant growth.

[0024] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S5, the initial shading rate of the shading net system is set to 80%, and is gradually adjusted to 60% as the Cistanche deserticola plants grow and the external light intensity changes. Finally, it is adjusted to 50% one month before harvesting, so as to simulate the light conditions in its natural habitat that vary with the canopy closure, and promote the accumulation of photosynthetic products.

[0025] By gradually and systematically reducing the shading rate, the dynamic changes in the light environment experienced by Cistanche deserticola from germination to maturity under native alder forests were accurately simulated. This avoided photoinhibition or excessive growth caused by sudden changes in light intensity, guided the plant to smoothly transition from vegetative growth to reproductive growth, and effectively promoted photosynthesis by moderately increasing light before harvest, maximizing the accumulation of medicinal active ingredients.

[0026] Preferably, in the above-mentioned method for cultivating Cistanche deserticola, in step S6, during the budding stage of Cistanche deserticola, the phosphorus and potassium fertilizer applied by drip irrigation is potassium dihydrogen phosphate with a concentration of 0.2%, and at the same time, boric acid with a concentration of 0.05% and zinc sulfate with a concentration of 0.02% are added, which can increase the content of phenylethanoid glycosides in Cistanche deserticola medicinal material.

[0027] During the budding stage, a critical period for the synthesis of medicinal components, the supply of phosphorus and potassium directly participates in and promotes energy metabolism and the synthesis and transport of secondary metabolites such as phenylethanoid glycosides; boron is essential for the transport of carbohydrates and the development of reproductive organs; zinc is a cofactor for many enzymes, and through the synergy of nutrients, it precisely regulates the metabolic flow of Cistanche deserticola, shifting from promoting vegetative growth to efficiently synthesizing target medicinal components, thereby directly and effectively improving the intrinsic quality and economic value of the final medicinal material.

[0028] As can be seen from the above technical solution, compared with the prior art, this invention discloses a method for cultivating Cistanche deserticola, realizing the transformation from traditional cultivation that relies on natural conditions to modern, precise, standardized, and factory-style production. It transfers the two most critical and uncontrollable core links in Cistanche deserticola cultivation—seed germination and parasitism—from the complex wild habitat to a laboratory environment for active intervention and precise control.

[0029] This invention utilizes the synergistic effect of specific light quality and natural signaling substances in the host root system to provide a stable and efficient comprehensive induction environment for seed germination, significantly improving germination vitality and uniformity. Through artificial guidance and standardized cultivation on a substrate simulating the natural rhizosphere structure, a Cistanche deserticola-Alnus symbiotic organism with uniform structure and stable parasitic connection is obtained. The standardized artificial parasitic seedlings produced are then subjected to systematic hardening and domestication, transplanting, intelligent microenvironmental regulation, and staged precise nutrient management, ensuring high survival rate, robust growth, and uniform quality of the plants throughout the entire growth process from transplanting to harvest.

[0030] This invention overcomes the inherent problems of existing technologies, such as over-reliance on specific natural habitats and the resulting low parasitism rate, long production cycle, and unstable yield and quality. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0032] Figure 1 The attached figure is a flowchart illustrating a method for cultivating Cistanche deserticola according to the present invention. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] This invention discloses a method for cultivating Cistanche deserticola, comprising the following steps: S1. Seed photo-chemical synergistic germination induction: Mature Cistanche deserticola seeds were collected, surface-sterilized, and placed in a non-sterile extract culture medium of Alder root solidified with agarose, and cultured in a light incubator. S2. Artificial Parasite Integration: The Cistanche deserticola seeds that successfully germinated and elongated their radicles in step S1 were transferred to a solid symbiotic culture medium. The solid symbiotic culture medium was covered with an ultrafine fiber mesh that simulated the surface structure of the root system. At the same time, the roots of the pre-cultured aseptic tissue culture seedlings of Aldera sibiricum were placed in the fiber mesh area, so that the radicles of Cistanche deserticola and the roots of Aldera sibiricum could come into natural contact in three-dimensional space. By controlling the carbon source and auxin concentration in the culture medium, the tips of the radicles of Cistanche deserticola were specifically adsorbed onto the roots of Aldera sibiricum and formed an artificial parasite. S3. Parasite hardening and seedling strengthening: The artificial parasites with stable parasitic connections from step S2 are transferred from the sterile environment to the seedling trays containing a light moisture-retaining substrate and cultivated in an environmental hardening room. By gradually reducing the air humidity and increasing the light intensity in a programmed manner, the parasites are adapted to the non-sterile environment, and their root development and plant vigor are promoted. S4. Site selection and transplanting: At the selected planting site, holes are drilled in the roots of the host alder according to the size of the artificial parasite's root system. The holes are backfilled with a planting substrate made of local soil, organic matter and water-retaining agent in a certain proportion. The healthy parasite seedlings are transplanted into the holes with their clumps intact, ensuring that their original parasitic connection structure and root system are intact. S5. Intelligent regulation of growth microenvironment: A shade net system with adjustable height and shading rate is built above the transplanting area. At the same time, soil temperature and humidity sensors and drip irrigation network are installed. Based on the sensor data feedback, irrigation and shading are automatically adjusted to create and maintain a relatively stable root zone and canopy microenvironment with light, temperature and humidity for the growth of Cistanche deserticola. S6. Precise staged nutrient supplementation: Based on the phenological stage of Cistanche deserticola's transformation from vegetative growth to reproductive growth, water-soluble fertilizers with different formulations are applied in a pulsed manner through the drip irrigation system. In the early stage of growth, nitrogen is emphasized to promote biomass accumulation, and in the early stage of flowering, phosphorus and potassium fertilizers and boron and zinc micro-fertilizers are applied to increase the content of medicinal components. S7. Target Harvesting and Regeneration Management: When the inflorescence of Cistanche deserticola has fully elongated but has not yet fully bloomed, use a sharp knife to cut and harvest the above-ground parts, retaining the underground corms and parasitic structures. Immediately after harvesting, spray with a fungicide and apply a root-promoting and shoot-growing fertilizer to nourish the underground parts and stimulate the sprouting of new, robust shoots.

[0035] This invention transforms the traditional wild parasitism process, which relies on uncertain natural conditions, into a standardized, highly controllable process in the laboratory through steps such as artificial germination induction, artificial parasitism integration, seedling hardening, customized transplanting, intelligent environmental control, precise nutrient management, and regeneration harvesting. Ultimately, it achieves high germination rate, high parasitism success rate, high survival rate, controllable growth cycle, consistent medicinal quality, and sustainable land use for Cistanche deserticola cultivation, fundamentally solving the core problems of low parasitism rate, unstable yield, and high susceptibility to natural environmental influences in existing technologies.

[0036] To further optimize the above technical solution, the illumination in the light incubator is provided by red LEDs and blue LEDs in a 7:3 ratio of photon flux density, with 12-16 hours of illumination per day. Through the synergistic effect of specific illumination light and the chemical signals of alder roots, seed germination is efficiently and controllably initiated and the radicle is guided to grow in a directional manner.

[0037] To further optimize the above technical solution, in step S1, the non-sterile extract of alder roots is prepared by the following method: take the fibrous roots of healthy one-year-old alder seedlings, wash them, and mix them with distilled water at a weight-to-volume ratio of 1:5-1:10. After soaking at 4℃ for 24-48 hours, filter and take the supernatant. The supernatant contains natural chemical signaling substances secreted by alder roots, which synergistically regulate germination with the light irradiation in the light incubator.

[0038] Low-temperature water extraction can maximize the acquisition and preservation of the complete set of natural chemical signaling substances secreted by alder roots that can induce the germination of Cistanche deserticola seeds. This avoids the problem of insufficient induction efficiency that may exist with single compounds, and provides a highly efficient and more natural comprehensive induction environment for seed germination, thereby significantly improving the germination rate and uniformity of germination.

[0039] To further optimize the above technical solution, in the light incubator, the peak wavelength of the red LED is 660±5nm and the peak wavelength of the blue LED is 450±5nm. This combination of light quality can not only effectively promote seed germination, but also guide the radicle of Cistanche deserticola to present a healthy morphology.

[0040] Red light at 660nm is the main absorption peak of the phytochrome Prf, which can effectively promote seed germination; blue light at 450nm participates in regulating plant morphogenesis and tropism. When the two are combined in a specific ratio, they not only drive the germination process by synergistically activating the light signaling pathway, but also guide the radicle to elongate healthily and in a directional manner, avoiding radicle malformation or growth stagnation.

[0041] To further optimize the above technical solution, in step S2, the ultrafine fiber mesh simulating the root surface structure is a polyester fiber nonwoven fabric with a roughened surface. Its fiber diameter is similar to that of alder root hairs, ranging from 10 to 20 micrometers. This provides physical contact stimulation to the embryonic roots of Cistanche deserticola and guides its search and contact path with alder roots.

[0042] The fiber web simulates the physical environment of alder root hairs in natural soil, providing key physical contact stimulation for the radicle of Cistanche deserticola. This stimulation, together with chemical signals, guides the radicle's searching behavior. The three-dimensional structure provides natural contact and entanglement space for both root systems, avoiding damage that may be caused by forced contact, thus promoting the formation of parasitic haustoria and successful parasitism more naturally and efficiently.

[0043] To further optimize the above technical solution, in step S2, the solid symbiotic culture medium is based on 1 / 2 MS medium, with the addition of indolebutyric acid at a concentration of 0.05-0.2 mg / L to promote the expansion of alder roots on the fiber network, while the sucrose concentration is controlled at 10-15 g / L to maintain an adequate carbon source supply without inhibiting the parasitic recognition process.

[0044] Adding a low concentration of indolebutyric acid effectively stimulates the rapid growth and expansion of alder roots within the fiber network, increasing the probability of contact with the radicle of Cistanche deserticola. Maintaining a low sucrose concentration provides the necessary energy for the initial growth of both species while avoiding the potential inhibitory effect of a high-sugar environment on parasitic recognition signaling pathways.

[0045] To further optimize the above technical solution, in step S3, the lightweight moisturizing substrate is made of coconut coir, vermiculite and biochar in a volume ratio of 6:3:1. Its porosity is greater than 60% and its pH value is stable between 5.8 and 6.5, providing good structural support and buffer protection for the parasite roots after they leave the sterile environment.

[0046] The substrate, formulated with a specific ratio, combines high porosity, excellent water retention, and a stable weakly acidic environment. The addition of biochar further improves the substrate structure and helps adsorb harmful substances. This substrate provides a physical buffer and a water-air coordinated transition environment for the fragile parasites after they are removed from agar support, greatly reducing stress during hardening and improving seedling vigor and transplant survival rate.

[0047] To further optimize the above technical solution, in step S4, the volume ratio of local soil, decomposed organic fertilizer and water-retaining agent in the planting substrate is 7:2:1. The water-retaining agent is polyacrylamide type, which can maintain the soil moisture content in the root zone at 60%-70% of the field capacity for a long time after the first saturation water absorption, effectively coping with short-term drought stress.

[0048] The planting substrate utilizes local soil to ensure environmental adaptability, while well-rotted organic fertilizer provides slow-release nutrients. After irrigation, it absorbs and locks in a large amount of water, slowly releasing it to the roots during drought. This characteristic allows the soil moisture in the root zone to be stably maintained within the optimal range for Cistanche deserticola growth, significantly reducing growth stress caused by water fluctuations. It is particularly suitable for mountainous or sloping areas where frequent irrigation is not possible, ensuring stable plant growth.

[0049] To further optimize the above technical solution, in step S5, the initial shading rate of the shading net system is set at 80%, and gradually adjusted to 60% as the Cistanche deserticola plants grow and the external light intensity changes. Finally, it is adjusted to 50% one month before harvesting to simulate the light conditions that vary with the canopy closure in its natural habitat and promote the accumulation of photosynthetic products.

[0050] By gradually and systematically reducing the shading rate, the dynamic changes in the light environment experienced by Cistanche deserticola from germination to maturity under native alder forests were accurately simulated. This avoided photoinhibition or excessive growth caused by sudden changes in light intensity, guided the plant to smoothly transition from vegetative growth to reproductive growth, and effectively promoted photosynthesis by moderately increasing light before harvest, maximizing the accumulation of medicinal active ingredients.

[0051] To further optimize the above technical solution, in step S6, during the budding stage of Cistanche deserticola, the phosphorus and potassium fertilizer applied by drip irrigation is potassium dihydrogen phosphate with a concentration of 0.2%, and at the same time, boric acid with a concentration of 0.05% and zinc sulfate with a concentration of 0.02% are added, which can increase the content of phenylethanoid glycoside active ingredients in Cistanche deserticola.

[0052] During the budding stage, a critical period for the synthesis of medicinal components, the supply of phosphorus and potassium directly participates in and promotes energy metabolism and the synthesis and transport of secondary metabolites such as phenylethanoid glycosides; boron is essential for the transport of carbohydrates and the development of reproductive organs; zinc is a cofactor for many enzymes, and through the synergy of nutrients, it precisely regulates the metabolic flow of Cistanche deserticola, shifting from promoting vegetative growth to efficiently synthesizing target medicinal components, thereby directly and effectively improving the intrinsic quality and economic value of the final medicinal material.

[0053] Technical principle of this invention: This invention utilizes biomimetic simulation and artificial enhancement to actively construct and standardize the production of Cistanche deserticola-Alnus symbiotic units in a controlled environment, thereby transforming the uncontrollable wild parasitic process in traditional agriculture into a highly controllable industrialized biomanufacturing process.

[0054] First, by comprehensively simulating and synergistically inducing environmental signals to replace single, uncertain natural signals, a specific ratio of red and blue light is used to simulate the forest undergrowth light environment. This is supplemented by a natural chemical signal set secreted by the host root system, forming a photo-chemical synergistic initiation signal that efficiently and directionally activates the germination process of *Cistanche deserticola* seeds. Based on germination, the artificial parasite is actively assembled and standardized. By introducing a physical matrix simulating the root surface, contact guidance and mechanical stimulation are provided to the *Cistanche deserticola* radicle, allowing it to naturally contact the *Alnus altissima* root system within a pre-designed three-dimensional space. During this process, by precisely controlling the nutrient and hormone levels of the symbiotic culture medium, an optimal biochemical microenvironment is created, actively guiding and completing the formation and functional connection of the parasitic haustorium, thus reliably establishing the parasitic relationship.

[0055] Finally, the established, standardized artificial parasites are placed within an intelligent agronomic management system based on environmental feedback. Through transplanting substrates, dynamically adjustable light and water supply, and precise nutrient intervention matching phenological stages, optimal growth conditions are provided for the established symbiotic units, ensuring a smooth transition from laboratory to field growth and efficient nutrient accumulation.

[0056] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0057] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cultivating Cistanche deserticola, characterized in that, Includes the following steps: S1. Seed photo-chemical synergistic germination induction: Mature Cistanche deserticola seeds were collected, surface-sterilized, and placed in a non-sterile extract culture medium of Alder root solidified with agarose, and cultured in a light incubator. S2. Artificial Parasite Integration: The Cistanche deserticola seeds that successfully germinated and elongated their radicles in step S1 were transferred to a solid symbiotic culture medium. The solid symbiotic culture medium was covered with an ultrafine fiber mesh that simulated the surface structure of the root system. At the same time, the roots of the pre-cultured aseptic tissue culture seedlings of Aldera sibiricum were placed in the fiber mesh area, so that the radicles of Cistanche deserticola and the roots of Aldera sibiricum could come into natural contact in three-dimensional space. By controlling the carbon source and auxin concentration in the culture medium, the tips of the radicles of Cistanche deserticola were specifically adsorbed onto the roots of Aldera sibiricum and formed an artificial parasite. S3. Parasite hardening and seedling strengthening: The artificial parasites with stable parasitic connections from step S2 are transferred from the sterile environment to the seedling trays containing a light moisturizing substrate and cultured in an environmental hardening room by gradually reducing air humidity and increasing light intensity in a programmed manner. S4. Site selection and transplanting: At the selected planting site, holes are drilled in the roots of the host alder according to the size of the artificial parasite's root system. The holes are backfilled with a planting substrate made of local soil, organic matter and water-retaining agent in a certain proportion. The healthy parasite seedlings are then transplanted into the holes with their clumps. S5. Intelligent control of growth microenvironment: A shade net system with adjustable height and shading rate is built above the transplanting area. At the same time, soil temperature and humidity sensors and drip irrigation network are installed. Irrigation and shading are automatically adjusted based on sensor data feedback. S6. Precise staged nutrient supplementation: Based on the phenological stage of Cistanche deserticola's transformation from vegetative growth to reproductive growth, water-soluble fertilizers with different formulations are applied in a pulsed manner through the drip irrigation system; S7. Target Harvesting and Regeneration Management: When the inflorescence of Cistanche deserticola has fully elongated but has not yet fully bloomed, use a sharp knife to cut and harvest the above-ground parts, retaining the underground corms and parasitic structures. Immediately after harvesting, spray with a fungicide and protectant and apply a root-promoting and shoot-growing fertilizer.

2. The method for cultivating Cistanche deserticola according to claim 1, characterized in that, The illumination in the light incubator is provided by a ratio of red LEDs to blue LEDs with a photon flux density of 7:3, with 12-16 hours of illumination per day.

3. A method for cultivating Cistanche deserticola according to claim 2, characterized in that, In step S1, the non-sterile extract of alder roots is prepared by the following method: take the fibrous roots of healthy one-year-old alder seedlings, wash them, mix them with distilled water at a weight-to-volume ratio of 1:5-1:10, extract at 4°C for 24-48 hours, and then filter to obtain the supernatant.

4. A method for cultivating Cistanche deserticola according to claim 2, characterized in that, The peak wavelength of the red LED is 660±5nm, and the peak wavelength of the blue LED is 450±5nm.

5. A method for cultivating Cistanche deserticola according to claim 4, characterized in that, In step S2, the ultrafine fiber mesh simulating the root surface structure is a polyester fiber nonwoven fabric with a roughened surface, and its fiber diameter is similar to that of alder root hair, ranging from 10 to 20 micrometers.

6. A method for cultivating Cistanche deserticola according to claim 5, characterized in that, In step S2, the solid symbiotic culture medium is based on 1 / 2 MS medium, with the addition of indolebutyric acid at a concentration of 0.05-0.2 mg / L, while the sucrose concentration is controlled at 10-15 g / L.

7. A method for cultivating Cistanche deserticola according to claim 6, characterized in that, The lightweight moisturizing matrix is ​​composed of coconut coir, vermiculite and biochar in a volume ratio of 6:3:1, with a porosity greater than 60% and a pH value stable between 5.8 and 6.

5.

8. A method for cultivating Cistanche deserticola according to claim 7, characterized in that, The volume ratio of local soil, well-rotted organic fertilizer and water-retaining agent in the planting substrate is 7:2:

1. The water-retaining agent is polyacrylamide type, which can maintain the soil moisture content in the root zone at 60%-70% of field capacity for a long time after the first saturation water absorption.

9. A method for cultivating Cistanche deserticola according to claim 8, characterized in that, The initial shading rate of the shade net system was set at 80%, which was gradually adjusted to 60% as the Cistanche deserticola plants grew and the external light intensity changed, and finally adjusted to 50% one month before harvest.

10. A method for cultivating Cistanche deserticola according to claim 1, characterized in that, In step S6, during the budding stage of Cistanche deserticola, the phosphorus and potassium fertilizer applied by drip irrigation is potassium dihydrogen phosphate with a concentration of 0.2%, and at the same time, boric acid with a concentration of 0.05% and zinc sulfate with a concentration of 0.02% are added.

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