Animal behavior experimental device for smoke exposure

Abstract

The invention belongs to the technical field of biological experiment equipment, and particularly provides an animal behavior experiment device for smoke exposure, which comprises an open field box body with an open top; the cover plate is detachably arranged at the top of the open field box body, and a closed experiment cavity is formed by the cover plate and the open field box body; the smoke generating device is connected with the open field box body through a pipeline and is used for conveying smoke to the experiment chamber; the behavior observation device and the optical genetic stimulation device are both integrally arranged on the cover plate, and the behavior observation device is matched with the smoke generation device and used for conducting behavioral observation when the animal is exposed to the smoke; and the photogenetic stimulation device is matched with the behavior observation device and is used for carrying out behavioral observation when the animals are subjected to photogenetic intervention. According to the device, environmental fluctuation, time delay and stress interference caused by animal transfer among different devices or step-by-step experiments in traditional research are overcome, and the research accuracy and efficiency are greatly improved.

Description

Technical Field

[0001] This application belongs to the field of biological experimental equipment technology, specifically relating to an animal behavior experimental device for smoke exposure. Background Technology

[0002] In animal experimental studies involving smoking behavior and air pollution exposure, the smoke exposure animal behavior experimental device is an important research tool. It is used to apply specific smoke stimuli to experimental animals under controlled conditions and observe and analyze their behavioral responses.

[0003] However, existing smoke exposure experiments typically use a separate smoke generator to expose mice to a specific concentration of smoke before transferring them to a behavioral testing device for behavioral evaluation. This separate procedure not only increases the complexity of the experiment but may also cause stress responses in the mice due to environmental transfer, affecting the accuracy of the experimental results. Summary of the Invention

[0004] To overcome the shortcomings of the prior art, this application proposes an animal behavior experiment device for smoke exposure, which is specifically implemented through the following technical solution: An animal behavior experiment apparatus for smoke exposure includes: An open-top enclosure; A cover plate is detachably mounted on the top of the open field box and forms a sealed experimental chamber with the open field box. A smoke generator is connected to the open space box via a pipe and is used to deliver smoke into the experimental chamber; Both the behavioral observation device and the optogenetic stimulation device are integrated on the cover plate. The behavioral observation device works in conjunction with the smoke generator to conduct behavioral observations while the animal is exposed to smoke. The optogenetic stimulation device works in conjunction with the behavioral observation device to conduct behavioral observations while the animal undergoes optogenetic intervention.

[0005] In an optional embodiment, the behavioral observation device is configured with connecting lines, and the optogenetic stimulation device has an optical fiber; the cover plate has through holes for the connecting lines and / or the optical fiber to pass through. The through-hole is provided with an elastic sealing structure, which has a slit that closes in its natural state. The slit is configured to allow the connecting line to pass through while maintaining the airtightness of the experimental chamber.

[0006] In an optional embodiment, the resilient sealing structure includes a first sealing gasket and a second sealing gasket connected together; both the first and second sealing gaskets have the slit, and the extension directions of the slits of the first and second sealing gaskets are perpendicular to each other.

[0007] In an optional embodiment, the behavior observation device includes a camera device for acquiring images and an illumination component for illuminating the camera device, wherein the camera device and the illumination component are separately configured and each is equipped with a connection line; The through hole includes a first through hole and a second through hole. The connection line of the camera device extends out of the experimental cavity through the first through hole. The elastic sealing structure at the first through hole is configured to allow the connection line of the camera device to pass through and maintain the airtightness of the experimental cavity. The connection line of the lighting component extends out of the experimental chamber through the second through hole, and the elastic sealing structure at the second through hole is configured to allow the connection line of the lighting component to pass through and maintain the airtightness of the experimental chamber.

[0008] In an optional embodiment, the lighting assembly includes a first lighting lamp and a second lighting lamp, which are respectively disposed on opposite edges of the cover plate; The camera device includes an infrared camera, which is located between the first light source and the second light source.

[0009] In an optional embodiment, the optogenetic stimulation device further includes an external laser, the through-hole includes a third through-hole, and the external laser is located outside the experimental cavity; One end of the optical fiber is connected to the external laser, and the other end of the optical fiber extends into the experimental cavity through the third through hole and extends for a predetermined length; the elastic sealing structure at the third through hole is configured to allow the optical fiber to pass through and maintain the airtightness of the experimental cavity.

[0010] In an optional embodiment, the edge of the cover plate is provided with a sealing strip, and the cover plate covers the top of the open space box and presses the sealing strip.

[0011] In an optional embodiment, the conduit includes an input pipe and an output pipe, which are respectively connected to the top of opposite side walls of the open space enclosure.

[0012] In an optional embodiment, the input pipe and / or the output pipe are provided with a flow control valve for adjusting the smoke flow rate; The output pipe is equipped with a filter device for purifying the discharged gas.

[0013] In an optional embodiment, the open space enclosure includes an opaque enclosure, and the cover plate includes an opaque cover plate.

[0014] This application has at least the following beneficial effects: This application proposes an animal behavior experiment device for smoke exposure. By integrating the behavioral observation device and the optogenetic stimulation device on the cover plate and working in conjunction with the smoke generator, it enables simultaneous smoke exposure to experimental animals in the same closed and controlled environment, as well as real-time behavioral observation and / or precise optogenetic neural intervention. This overcomes the environmental fluctuations, time delays, and stress interference caused by the need to transfer animals between different devices or conduct step-by-step experiments in traditional research. It achieves a high degree of uniformity of experimental conditions and instantaneous correlation of data, greatly improving the accuracy and efficiency of the research. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of an animal behavior experiment device for smoke exposure according to this application. Figure 1 ; Figure 2 This is a schematic diagram of an animal behavior experiment device for smoke exposure according to this application. Figure 2 ; Figure 3 This is a schematic diagram of an animal behavior experiment device for smoke exposure according to this application. Figure 3 ; Figure 4 This is a schematic diagram of an animal behavior experiment device for smoke exposure according to this application. Figure 4 ; Figure 5 This is a schematic diagram of an animal behavior experiment device for smoke exposure according to this application. Figure 5 .

[0017] Figure label: 1-Opening box; 2-Cover plate; 211 - First through hole; 212 - Second through hole; 213 - Third through hole; 22-Elastic sealing structure; 221-Slit; 222-First sealing gasket; 223-Second sealing gasket; 3-Smoke generating device; 311 - Input transistor; 312 - Output transistor; 4-Behavioral observation device; 41-Camera device; 42-Lighting assembly; 421 - First lighting lamp; 422 - Second lighting lamp; 5-Optical genetic stimulation device; 51 - Fiber optic cable; 52 - External laser; 6-Experimental chamber; 71 - Connection lines for the camera device; 72 - Connection lines for the lighting components. Detailed Implementation

[0018] Various embodiments of this application will be described more fully below. This application may have various embodiments, and adjustments and changes may be made therein. However, it should be understood that there is no intention to limit the various embodiments of this application to the specific embodiments disclosed herein, but rather this application should be understood to cover all adjustments, equivalents, and / or alternatives falling within the spirit and scope of the various embodiments of this application.

[0019] In the following, the terms “comprising” or “may include” as used in the various embodiments of this application indicate the presence of the disclosed functions, operations, or elements, and do not limit the addition of one or more functions, operations, or elements. Furthermore, as used in the various embodiments of this application, the terms “comprising,” “having,” and their cognates are intended only to indicate a particular feature, number, step, operation, element, component, or combination of the foregoing, and should not be construed as primarily excluding the presence of one or more other features, numbers, steps, operations, elements, components, or combinations of the foregoing, or the possibility of adding one or more combinations of features, numbers, steps, operations, elements, components, or combinations of the foregoing.

[0020] like Figure 1 As shown, an animal behavior experiment apparatus for smoke exposure includes: Open space box 1, the top of open space box 1 is open; The cover plate 2 is detachably installed on the top of the open field box 1 and forms a closed experimental chamber 6 with the open field box 1. The smoke generator 3 is connected to the open space box 1 through a pipe and is used to deliver smoke to the experimental chamber. The behavioral observation device 4 and the optogenetic stimulation device 5 are both integrated on the cover plate 2. The behavioral observation device 4 works in conjunction with the smoke generator 3 to conduct behavioral observations while the animal is exposed to smoke. The optogenetic stimulation device 5 works in conjunction with the smoke generator 3 to conduct optogenetic interventions while the animal is exposed to smoke.

[0021] The animal behavior experiment device for smoke exposure in this application integrates the behavior observation device 4 and the optogenetic stimulation device 5 on the cover plate 2 and works in conjunction with the smoke generating device 3. This enables simultaneous smoke exposure to experimental animals in the same closed and controlled environment, with real-time behavioral observation and / or precise optogenetic neural intervention. It overcomes the environmental fluctuations, time delays, and stress interference caused by the need to transfer animals between different devices or conduct step-by-step experiments in traditional research. It achieves a high degree of uniformity of experimental conditions and instantaneous correlation of data, greatly improving the accuracy and efficiency of the research.

[0022] The open field chamber 1 comprises an opaque chamber, forming a cubic open field. The cover plate 2 comprises an opaque cover plate 2, which can completely cover the top of the cubic open field. The use of opaque materials for the open field chamber 1 and cover plate 2 in this application helps to reduce the interference of external ambient light on the internal experiments, making the experimental environment more controllable.

[0023] Furthermore, such as Figures 1-5 As shown, the behavioral observation device 4 is equipped with connecting lines, and the optogenetic stimulation device 5 has an optical fiber 51; the cover plate 2 has through holes for the connecting lines and / or optical fiber 51 to pass through.

[0024] An elastic sealing structure 22 is provided at the through hole. The elastic sealing structure 22 has a slit 221 that closes in its natural state. The slit 221 is configured to allow the connecting line to pass through and maintain the airtightness of the experimental chamber 6.

[0025] Among them, the elastic sealing structure 22 is a silicone sealing layer, which is attached to the top of the channel hole opened on the open field cover plate 2.

[0026] This application provides an elastic sealing structure 22 with a naturally closing slit 221 at the through hole of the cover plate 2. While allowing the connecting lines of the behavioral observation and photogenetic stimulation device 5 to pass through flexibly, the slit 221 effectively maintains the high airtightness of the experimental chamber 6 by virtue of its natural closing characteristics and the elastic tight wrapping during threading. This ensures the stability and uniformity of the smoke concentration during the experiment and prevents the leakage of harmful smoke.

[0027] like Figures 1-5 As shown, the elastic sealing structure 22 includes a first sealing gasket 222 and a second sealing gasket 223, which are connected together. Both the first sealing gasket 222 and the second sealing gasket 223 have slits 221, and the extending directions of the slits 221 of the first sealing gasket 222 and the second sealing gasket 223 are perpendicular to each other. The slits 221 on the first sealing gasket 222 and the second sealing gasket 223 are cross-shaped.

[0028] This application, by setting a first sealing gasket 222 and a second sealing gasket 223 with mutually perpendicular slits 221, implements cross- and multi-directional elastic compression and wrapping of the connecting line passing through, which significantly improves the static sealing reliability and dynamic following sealing capability at the through hole, and realizes the long-term and absolute airtightness of the experimental chamber 6 under various working conditions.

[0029] like Figures 1-5 As shown, the behavioral observation device 4 includes a camera device 41 for acquiring images and an illumination assembly 42 for illuminating the camera device 41. The camera device 41 and the illumination assembly 42 are separately arranged and each is equipped with a connection line. The through-holes include a first through-hole 211 and a second through-hole 212. The connection line 71 of the camera device extends out of the experimental chamber 6 through the first through-hole 211. The elastic sealing structure 22 at the first through-hole 211 is configured to allow the connection line 71 of the camera device to pass through while maintaining the airtightness of the experimental chamber 6. The connection line 72 of the illumination assembly extends out of the experimental chamber 6 through the second through-hole 212. The elastic sealing structure 22 at the second through-hole 212 is configured to allow the connection line 72 of the illumination assembly to pass through while maintaining the airtightness of the experimental chamber 6.

[0030] This application decouples the camera and lighting functions of the behavior observation device 4, and provides independent through holes and corresponding elastic sealing structures 22 for the camera device 41 and the lighting component 42 respectively. This allows the camera and lighting component 42 to be independently arranged in the optimal position of the cover plate 2 according to optical requirements, avoiding light interference and field of view obstruction that may occur with co-location, and significantly improving the clarity of image acquisition and the accuracy of behavior observation data. Furthermore, through line splitting, each through hole only needs to pass through a single or a small number of lines, effectively reducing the wiring density and sealing burden of a single sealing structure. This allows the elastic sealing slit 221 to form a tighter and more reliable wrap around the lines, greatly enhancing the overall sealing effect and completely eliminating the inconvenience of installation and maintenance caused by multiple lines tangling together.

[0031] like Figures 1-5 As shown, the lighting assembly 42 includes a first light 421 and a second light 422, which are respectively disposed on opposite edges of the cover plate 2. The camera device 41 includes an infrared camera, which is located between the first light 421 and the second light 422.

[0032] This application, by placing the first illumination lamp 421 and the second illumination lamp 422 on opposite sides of the cover plate 2 and centering the infrared camera between them, presents a symmetrical layout and a central observation configuration. This configuration can form a uniform and stable illumination field within the experimental chamber 6, minimizing blind spots and shadow interference, and providing the infrared camera with a clear panoramic and detailed imaging environment. This ensures high quality and data reliability of behavioral observation images under smoke exposure conditions.

[0033] like Figures 1-5 As shown, the optogenetic stimulation device 5 also includes an external laser 52, and the through-hole includes a third through-hole 213. The external laser 52 is located outside the experimental chamber 6. One end of an optical fiber 51 is connected to the external laser 52, and the other end of the optical fiber 51 extends into the experimental chamber 6 through the third through-hole 213 and extends for a predetermined length. The length of the optical fiber 51 is sufficient to allow an animal (such as a mouse) to move freely within the open field chamber 1 after carrying the implanted device. The elastic sealing structure 22 at the third through-hole 213 is configured to allow the optical fiber 51 to pass through while maintaining the airtightness of the experimental chamber 6.

[0034] This application places the laser, which generates significant heat and electromagnetic interference, outside the experimental cavity 6, fundamentally avoiding the impact of the light source's own thermal radiation on the temperature field and animal behavior within the cavity. Simultaneously, it eliminates the potential interference of equipment operation on the smoke flow field and observation optical path within the confined space, ensuring a highly pure and stable environment for behavioral observation and smoke exposure. Furthermore, the specially designed third through-hole 213 and the matching elastic sealing structure 22 provide a reliable and flexible seal tailored to the size and physical characteristics of the optical fiber 51. While ensuring the experimental cavity 6 is strictly sealed and preventing smoke leakage, it allows the optical fiber 51 to be flexibly and securely introduced, protecting the fragile optical fiber 51 line while maintaining the efficient and precise transmission of the optogenetic stimulation optical path.

[0035] In this embodiment, the cover plate 2 has a first through hole 211, two second through holes 212, and a third through hole 213, arranged linearly. The connection lines of the infrared camera pass through the first through hole 211, the connection lines of the first illumination lamp 421 and the second illumination lamp 422 pass through different second through holes 212, and the optical fiber 51 passes through the third through hole 213. This specific linear layout makes the connection lines of each functional module clear and orderly separated, fundamentally avoiding the crossing and tangling of multiple lines in a limited space, greatly simplifying the complexity of installation, debugging, and subsequent maintenance. Furthermore, the spatial separation of the lines effectively reduces the crosstalk between electromagnetic signals and heat, especially ensuring the purity of the infrared camera signal transmission and the stability of the imaging.

[0036] A sealing strip (not shown in the figure) is provided on the edge of the cover plate 2. The cover plate 2 covers the top of the open field chamber 1 and compresses the sealing strip. By providing a sealing strip on the edge of the cover plate 2 and utilizing the compression formed when the cover plate 2 covers the open field chamber 1, this application ensures the complete airtightness of the experimental chamber 6 at the circumferential edge, significantly preventing smoke leakage and ensuring high stability and spatial uniformity of smoke concentration during the experiment.

[0037] like Figures 1-5 As shown, the pipeline includes an input pipe 311 and an output pipe 312, which are respectively connected to the top of opposite side walls of the open-field chamber 1. This application, through a symmetrical bottom pipe layout, can guide the formation of a stable and uniform transverse smoke flow field within the experimental chamber 6, effectively avoiding eddies, dead zones, or stratification phenomena that may occur due to unidirectional input or top emission of smoke. This ensures a high degree of consistency and stability in the spatial and temporal smoke exposure concentration received by the experimental animals throughout the chamber.

[0038] Furthermore, a fastener is provided at the connection between the input pipe 311 and the open-field housing 1, which fixes the end of the input pipe 311 to the open-field housing 1. In addition, to ensure the airtightness of the inside of the open-field housing 1, a smoke leakage prevention accessory is also provided at the connection between the input pipe 311 and the open-field housing 1, thereby further improving the stability and reliability of the equipment.

[0039] The input pipe 311 and / or output pipe 312 are equipped with flow control valves for adjusting the smoke flow rate. This application enables researchers to actively, in real-time, and precisely control the smoke inflow rate, outflow rate, and ventilation efficiency within the experimental chamber 6 via these flow control valves, thereby achieving precise control over the smoke concentration, exposure duration, and cumulative exposure dose within the chamber. This not only ensures a high degree of consistency and repeatability of smoke exposure conditions between different batches or experimental groups, effectively improving the comparability and reliability of experimental data, but also greatly enhances the experimental flexibility of the device, allowing it to flexibly simulate various real-world smoke exposure scenarios, from constant low-dose exposure to pulsed high-dose exposure.

[0040] like Figures 1-5 As shown, the output pipe 312 is equipped with a filter device for purifying the discharged gas. The filter device of this application can effectively purify the gas containing smoke particles and potentially harmful components before it is discharged into the external environment, thereby completely preventing the pollution of the laboratory environment by experimental waste gas.

[0041] In one embodiment, the filtration device is an activated carbon filter canister to purify the discharged waste gas.

[0042] The specific usage process of the animal behavior experimental device for smoke exposure in this application is as follows: During the smoke exposure open field experiment, the operator can open the cover plate 2, attach the camera device 41 to the elastic sealing structure 22 close to the center, and connect the connecting wires through the slit 221 of the elastic sealing structure 22 to an external computer; the lighting components 42 connected to both ends of the cover plate 2 are also connected to an external power source through the elastic sealing structure 22, and the required lighting conditions are adjusted. Before the experiment begins, the experimental animal is placed in the open field box 1, and the smoke type, concentration, and exposure time are set on the smoke generating device 3. After the required concentration is reached, the changes in the experimental animal's behavior are observed and recorded through the camera device 41.

[0043] If optogenetic experiments are required, the animal with the implanted optical fiber is first placed in the open field box 1, the optical fiber 51 is connected to the external laser 52 through the slit 221 of the corresponding elastic sealing structure 22, and then the cover plate 2 is closed, and the above operation is repeated.

[0044] After the experiment, the residual smoke is discharged through the output pipe 312. The operator can then lift the cover plate 2, remove the experimental animals, and clean the open space box 1 in preparation for the next experiment.

[0045] In various embodiments of this application, the expression "or" or "at least one of A and / or B" includes any combination or all combinations of the words listed simultaneously. For example, the expression "A or B" or "at least one of A and / or B" may include A, may include B, or may include both A and B.

[0046] The terms used in the various embodiments of this application (such as "first," "second," etc.) may modify various constituent elements in the various embodiments, but do not limit the corresponding constituent elements. For example, the above terms do not limit the order and / or importance of the elements. The above terms are only used for the purpose of distinguishing one element from other elements. For example, a first user device and a second user device refer to different user devices, although both are user devices. For example, without departing from the scope of the various embodiments of this application, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element.

[0047] It should be noted that, in this application, unless otherwise explicitly specified and defined, terms such as "installation," "connection," and "fixation" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0048] In this application, those skilled in the art should understand that the terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings and are only for the purpose of describing this application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0049] The terminology used in the various embodiments of this application is for the purpose of describing particular embodiments only and is not intended to limit the various embodiments of this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which the various embodiments of this application pertain. The terms (such as those defined in a generally used dictionary) are to be interpreted as having the same meaning as in the context of the relevant technical field and are not to be interpreted as having an idealized or overly formal meaning, unless clearly defined in the various embodiments of this application.

Claims

1. An animal behavior experiment apparatus for smoke exposure, characterized in that, include: An open-top enclosure; A cover plate is detachably mounted on the top of the open field box and forms a sealed experimental chamber with the open field box. A smoke generator is connected to the open space box via a pipe and is used to deliver smoke into the experimental chamber; Both the behavioral observation device and the optogenetic stimulation device are integrated on the cover plate. The behavioral observation device works in conjunction with the smoke generator to conduct behavioral observations while the animal is exposed to smoke. The optogenetic stimulation device works in conjunction with the behavioral observation device to conduct behavioral observations while the animal undergoes optogenetic intervention.

2. The animal behavior experimental apparatus for smoke exposure according to claim 1, characterized in that, The behavioral observation device is equipped with a connection line, and the optogenetic stimulation device has an optical fiber; the cover plate has a through hole for the connection line and / or the optical fiber to pass through; The through-hole is provided with an elastic sealing structure, which has a slit that closes in its natural state. The slit is configured to allow the connecting line to pass through while maintaining the airtightness of the experimental chamber.

3. The animal behavior experimental apparatus for smoke exposure according to claim 2, characterized in that, The elastic sealing structure includes a first sealing gasket and a second sealing gasket, which are connected together; both the first sealing gasket and the second sealing gasket have the slit, and the extension directions of the slit of the first sealing gasket and the slit of the second sealing gasket are perpendicular to each other.

4. The animal behavior experimental apparatus for smoke exposure according to claim 2, characterized in that, The behavior observation device includes a camera device for acquiring images and an illumination component for illuminating the camera device. The camera device and the illumination component are set separately and each is equipped with a connection line. The through hole includes a first through hole and a second through hole. The connection line of the camera device extends out of the experimental cavity through the first through hole. The elastic sealing structure at the first through hole is configured to allow the connection line of the camera device to pass through and maintain the airtightness of the experimental cavity. The connection line of the lighting component extends out of the experimental chamber through the second through hole, and the elastic sealing structure at the second through hole is configured to allow the connection line of the lighting component to pass through and maintain the airtightness of the experimental chamber.

5. The animal behavior experimental apparatus for smoke exposure according to claim 4, characterized in that, The lighting assembly includes a first lighting lamp and a second lighting lamp, which are respectively disposed on opposite edges of the cover plate; The camera device includes an infrared camera, which is located between the first light source and the second light source.

6. The experimental apparatus for animal behavior testing in response to smoke exposure according to claim 2 or 4, characterized in that, The optogenetic stimulation device further includes an external laser, and the through-hole includes a third through-hole, with the external laser located outside the experimental cavity; One end of the optical fiber is connected to the external laser, and the other end of the optical fiber extends into the experimental cavity through the third through hole and extends for a predetermined length; the elastic sealing structure at the third through hole is configured to allow the optical fiber to pass through and maintain the airtightness of the experimental cavity.

7. The animal behavior experimental apparatus for smoke exposure according to claim 1, characterized in that, The cover plate is provided with a sealing strip on its edge, and the cover plate covers the top of the open space box and presses the sealing strip.

8. The animal behavior experimental apparatus for smoke exposure according to claim 1, characterized in that, The pipeline includes an input pipe and an output pipe, which are respectively connected to the top of opposite side walls of the open space enclosure.

9. The animal behavior experimental apparatus for smoke exposure according to claim 8, characterized in that, The input pipe and / or the output pipe are provided with flow control valves for adjusting the smoke flow rate; The output pipe is equipped with a filter device for purifying the discharged gas.

10. The animal behavior experimental apparatus for smoke exposure according to claim 1, characterized in that, The open space enclosure includes an opaque enclosure, and the cover plate includes an opaque cover plate.

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