Hair-blacking dietary composition and preparation method thereof

Abstract

The present invention discloses a hair-blacking dietary composition and a preparation method thereof, and relates to the technical field of functional food science, traditional Chinese medicine dietary therapy modernization and dermatobiology cross technology, the hair-blacking dietary composition comprises the following components by mass: 15-20% of first composite powder, 12-18% of second composite powder, 1-6% of third composite powder, and 2-8% of fourth composite powder, the first composite powder comprises rhizoma polygonati, radix astragali, radix angelicae sinensis and pericarpium citri reticulatae, the second composite powder comprises nostoc sphaeroids kutz, black mulberries and fructus lycii, the third composite powder comprises kelp and fructus alpiniae oxyphyllae, and the fourth composite powder comprises semen ziziphi spinosae and herba cistanche. The hair-blacking dietary composition can improve the hair-blacking effect and efficiency and promote hair growth.

Description

Technical Field

[0001] This application relates to the interdisciplinary fields of functional food science, modernization of traditional Chinese medicine diet therapy, and skin biology, and more specifically, to a hair-darkening dietary composition and its preparation method. Background Technology

[0002] Gray hair, especially premature graying, is a complex phenomenon involving genetics, imbalance of internal organs, oxidative stress, trace element deficiency, psychological factors, and dysfunction of the hair follicle microenvironment. Existing hair-darkening products suffer from the following systemic defects, resulting in limited, unstable, and unclear mechanisms of action:

[0003] First, it is a mere accumulation of ingredients rather than a systematic construction: existing solutions often simply mix or extract known hair-darkening ingredients such as black sesame, Polygonum multiflorum, and mulberry, which is a nutritional supplement approach and fails to design a multi-level compatibility and synergistic process with intrinsic functional logic based on the systemic needs of hair follicle melanocytes for survival, synthesis, and signal transduction.

[0004] Second, the crude process leads to loss of activity and low utilization: traditional processes such as high-temperature extraction and long-term boiling are very easy to destroy heat-sensitive components and cannot convert the key nutrients in the raw materials into bioavailable forms.

[0005] Third, there is a lack of systematic intervention on the gut-skin axis and the neuro-endocrine-hair follicle axis: existing products mostly focus on directly nourishing hair follicles, ignoring the gut microbiota that affects nutrient absorption and metabolite production, as well as the systematic regulation that affects the hair follicle cycle through the HPA axis, resulting in a single dimension of intervention.

[0006] Fourth, there is a lack of modern scientific explanation and in-depth verification: traditional formulations are highly empirical, but they cannot explain how the components work together or through which signaling pathways at the molecular and cellular level, and they lack direct comparative evidence with conventional solutions.

[0007] Therefore, developing hair-darkening dietary combinations through a four-level synergistic network encompassing localized anti-oxidation, systemic support, nutrient conversion, and homeostasis regulation to improve hair-darkening efficiency and effectiveness is a technical problem that needs to be solved. Summary of the Invention

[0008] This invention provides a hair-darkening dietary composition that systematically regulates the microenvironment for melanin synthesis in hair follicles through a four-level synergistic enhancement preparation technology. More specifically, this invention discloses a four-level functional base material formulation system with Polygonatum sibiricum-Astragalus membranaceus-Angelica sinensis-Citrus reticulata peel (first synergy), Nostoc commune-Black mulberry-Goji berry (second synergy), Laminaria japonica-Alpinia oxyphylla (third synergy), and Ziziphus jujuba var. spinosa-Cistanche deserticola (fourth synergy) as the core, and the composite product prepared by specific low-temperature co-soaking, time-coupled extraction, water-soil co-frying, and wine-soil co-processing processes to preserve structure and efficacy. It aims to solve the problem of non-pathological gray hair caused by nutritional signal transduction disorders, oxidative stress, and systemic dysfunction from multiple dimensions such as anti-oxidation, system regulation, trace element supplementation, and neuro-endocrine regulation.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] In a first aspect, the present invention provides a hair-darkening dietary composition comprising the following components by weight percentage: 15-20% of a first compound powder, 12-18% of a second compound powder, 1-6% of a third compound powder, and 2-8% of a fourth compound powder;

[0011] The first compound powder includes Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel; the second compound powder includes Nostoc commune, black mulberry, and wolfberry; the third compound powder includes Laminaria japonica and Alpinia oxyphylla; and the fourth compound powder includes Ziziphus jujuba var. spinosa and Cistanche deserticola.

[0012] Furthermore, in the first compound powder, the mass ratio of Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata is 1:0.3-0.4:0.3-0.6:0.1-0.3;

[0013] In the second compound powder, the mass ratio of Nostoc commune, black mulberry, and wolfberry is 1:3-4:0.5-0.8;

[0014] In the third composite powder, the mass ratio of kelp to Alpinia oxyphylla is 1:0.8-1.2;

[0015] In the fourth composite powder, the mass ratio of jujube seed to cistanche is 1:0.4-0.7.

[0016] Furthermore, it also includes: 14-16% black sesame powder, 5-6% black bean peptide powder, 3-5% walnut kernel and lotus seed powder, 2-3% Ganoderma lucidum spore powder, 2-3% black bean husk, 2-3% inulin, 2-3% black garlic extract, 1-2% yam powder, 1-2% Rehmannia glutinosa powder, 1-1.5% mulberry leaf powder, 0.5-1.0% microencapsulated B vitamins, 0.5-1.0% anti-caking agent, and fillers to bring the total to 100%.

[0017] Further, the preparation method of the walnut kernel and lotus seed powder includes: simmering a mixture of walnut kernels and lotus seeds in a decoction of chrysanthemum and hawthorn for 10-20 minutes, then removing, drying, and pulverizing. Preferably, the ratio of chrysanthemum to hawthorn is 1:1, with 20 times the amount of water added and boiled for 15 minutes, then filtered. A mixture of walnut kernels and lotus seeds in a 2:1 ratio is added to the above decoction and simmered for 15 minutes. The mixture is then removed, dried at 60℃ until the moisture content is ≤7%, and pulverized through an 80-mesh sieve. Chrysanthemum flavonoids are natural surfactants that, together with hawthorn acid, construct an in-situ natural emulsification system, optimizing the release form of walnut oil and lotus seed starch.

[0018] Secondly, the present invention also provides a method for preparing a hair-darkening dietary composition, comprising the following steps:

[0019] a) Preparation of the first compound powder: Astragalus membranaceus, Angelica sinensis, and tangerine peel are mixed with water, boiled and maintained at a gentle boil for 5 minutes, Polygonatum sibiricum is added, and heating is stopped immediately after boiling again. The mixture is soaked together for 2 hours, and solid-liquid separation is performed to obtain solid and extract. The solid is dried and then pulverized together to obtain the first compound powder.

[0020] b) Preparation of the second composite powder: Ge Xian Mi, black mulberry, and wolfberry are put into the extract solution at a temperature maintained at 45-50℃, soaked together at a constant temperature for 1.5 hours, dried and then pulverized together to obtain the second composite powder;

[0021] c) Preparation of the third compound powder: Kelp and Alpinia oxyphylla are mixed, boiled together with water for 0.5 to 1.5 hours, then removed and stir-fried together with calcined loess at 100 to 120°C for 15 to 25 minutes. The loess is then sieved out, cooled, and then pulverized together.

[0022] d) Preparation of the fourth compound powder: Soak jujube seed and cistanche in 40-60 degree liquor for 48-96 hours, then take them out and stir-fry them together with calcined loess at a temperature of 110-130℃ for 10-20 minutes. Sift out the loess, cool and then grind them together.

[0023] e) Mix the first composite powder, the second composite powder, the third composite powder, and the fourth composite powder with black sesame powder, black bean peptide powder, walnut kernel powder, Ganoderma lucidum spore powder, black bean husk, inulin, black garlic extract, yam powder, Rehmannia glutinosa powder, mulberry leaf powder, microencapsulated B vitamins, anti-caking agent, and filler to obtain the final product.

[0024] It should be noted that in step a), the extract is rich in astragalus polysaccharides, angelica ferulic acid, polygonatum oligosaccharides, tangerine peel flavonoids and other bioactive molecules that nourish qi, blood and yin.

[0025] In step b), on the one hand, under a low-temperature environment of 45℃, the reducing sugars in Astragalus membranaceus and Polygonatum sibiricum undergo a slow initial Maillard reaction to generate intermediates such as highly reactive α-dicarbonyl compounds. At this time, the black mulberry anthocyanins (strong nucleophiles) in the system are not passive antioxidants, but actively intercept these intermediates, undergoing nucleophilic addition reactions to generate novel anthocyanin-sugar derivatives and more complex anthocyanin-sugar-amino acid adducts. The enlarged hydrophobic framework and newly added functional groups of the anthocyanin-sugar derivatives can form multiple complementary interactions with the active site of tyrosinase (strong π-π stacking, hydrophobic interaction, potential coordination bonds), constructing a molecular scaffold, physically stabilizing the active conformation of the enzyme protein, resulting in a significant increase in the content of the enzyme's α-helix structure and an order-of-magnitude extension of the catalytic half-life. As an ultra-stable molecular scaffold, it directly locks in tyrosinase, fundamentally enhancing the function of the core engine of melanin synthesis. The unique conformation of this new molecule enables it to more effectively activate the crucial Nrf2 / ARE signaling pathway within cells, powerfully initiating the transcription of a series of endogenous antioxidant enzyme genes, such as superoxide dismutase (SOD) and glutathione peroxidase (GPx). This transforms the oxidative defense mode of hair follicle melanocytes from relying on external aid to self-arming, establishing a durable and fundamental antioxidant microenvironment. Enhanced melanin synthesis further strengthens the activity of tyrosinase in melanocytes, prompting them to secrete melanin, allowing black hair to grow from the roots of gray hair. On the other hand, during the co-soaking process, various polysaccharides (from kudzu root, astragalus, and polygonatum), polyphenols (anthocyanins and flavonoids), and trace amounts of proteins / peptides spontaneously and orderly self-assemble through non-covalent forces such as hydrogen bonds and hydrophobic interactions to form a polysaccharide-polyphenol-protein composite nanostructure with uniform nanoscale size. This structure is a natural bioactive carrier that can resist excessive degradation in the upper digestive tract, reach the colon intact, and be preferentially utilized by specific beneficial bacteria (such as Trichophyton and Bifidobacterium) as a selective substrate, thereby significantly optimizing the bacterial community structure and directionally promoting the production of its metabolites—short-chain fatty acids (especially butyrate). The direct activation of hair follicle stem cells by butyrate, a key messenger in polysaccharide-polyphenol-protein composite nanostructure: Butyrate absorbed into the bloodstream acts as an important systemic signaling molecule, reaching the hair follicle and directly acting on the root cause of hair regeneration. Specifically, it does so in three ways: First, through epigenetic regulation: inhibiting histone deacetylase, allowing the chromatin of hair follicle stem cells to open, directly activating key genes that maintain stemness and promote proliferation; second, through activation of core growth pathways: upregulating the activity of the Wnt / β-catenin signaling pathway in hair follicle stem cells, which is the core molecular switch driving hair follicles from the resting phase to the growth phase; and third, through improving the local microenvironment: providing energy to hair follicle cells and exerting anti-inflammatory effects, creating a hair follicle microenvironment conducive to regeneration and differentiation.

[0026] Specifically, in step c), kelp and Alpinia oxyphylla are mixed, and 10 times the volume (w / v) of purified water is added. The mixture is then refluxed at a gentle boil for 60 minutes. The kelp and Alpinia oxyphylla are removed, centrifuged, and drained. Calcined loess (calcined at 400℃ for 2 hours and passed through a 40-mesh sieve) is heated to 110℃ in a stir-frying pan. The drained mixture is added and stir-fried over low heat for 20 minutes until the surface of the material is dry, the color deepens, and a caramelized aroma is emitted. The mixture is quickly removed from the pan, the loess is sieved out, and the stir-fried material is spread out to cool to room temperature. The cooled kelp and Alpinia oxyphylla are then pulverized together and passed through a 100-mesh sieve. This co-cooking pre-compounding process allows some of the volatile components of Alpinia oxyphylla to penetrate the kelp tissue, while trace elements such as iodine and zinc in the kelp dissolve into the decoction and are adsorbed by Alpinia oxyphylla, achieving a preliminary component exchange. The abundant silicon, aluminum, iron, calcium and other oxides in loess can react with the organic acids, polysaccharides and trace elements in kelp during the heating process. This can promote the transformation of trace elements from inorganic to organic complex state and fix the warm components of Alpinia oxyphylla, producing a synergistic effect of soil-catalyzed transformation and warm-natured mineral absorption.

[0027] In step d), the jujube seed and cistanche are mixed and completely submerged in 50-degree (v / v) pure grain liquor. The mixture is placed in a sealed container and soaked at 25±5℃ for 72 hours, stirring once daily. The herbs are then removed and drained. Calcined loess is heated to 120℃ in a stir-frying pan, and the liquor-soaked herbs are added and stir-fried over low heat for 15 minutes until the surface of the herbs is uniformly deep yellow, swollen, and emits a rich, mixed, caramelized aroma. The herbs are quickly removed from the pan, sieved to completely separate the loess, and the stir-fried mixture is spread out to cool to room temperature. The cooled jujube seed and cistanche are then pulverized together and passed through a 90-mesh sieve. Ethanol, as a moderately polar solvent, can simultaneously and efficiently extract fat-soluble saponins (such as spinosin and jujube seed saponin A) from jujube seed and phenylethanol glycosides (such as echinacoside and verbascoside) from cistanche, achieving simultaneous dissolution and co-enrichment of the core active ingredients of both herbs on their surface. Calcination of loess provides a uniform and mild thermal field, which heat-treats the mixed active ingredients enriched on the surface, promoting esterification, cyclization, and other reactions to generate new flavor compounds and potentially more active derivatives. The mineral surface of loess can also act as a catalyst or adsorbent in the reaction process. This process aims to achieve the coupling of the calming (Ziziphus jujuba seed) and kidney-warming (Cistanche deserticola) effects at the molecular level, enhancing their regulatory effect on the neuroendocrine system.

[0028] In step e), the black sesame powder is preferably ultrafine black sesame powder, prepared by steaming with vinegar and ginger. Specifically, edible rice vinegar (total acid ≥ 5.0 g / 100 mL) and fresh ginger juice are mixed at a volume ratio of 95:5, and black sesame seeds are mixed with the vinegar and ginger liquid at a ratio of 1:2 (w / v). The mixture is then soaked at room temperature for 60 minutes. The mixture, along with the vinegar and ginger liquid, is heated to a boil and simmered for 10 minutes. The sesame seeds are then removed and steamed under normal pressure for 30 minutes. The mixture is dried at 50±2℃ until the moisture content is ≤5%, and then air-jet pulverized to a D90 ≤ 45 μm. Acetic acid chelates phytic acid, releasing minerals; gingerol promotes osmosis; steaming fixes nutrients; and ultrafine pulverization greatly improves bioavailability.

[0029] Preparation method of black soybean peptide powder: Black soybean powder was extracted with pH 5.0 acetic acid solution (containing 1% clove oil inclusion complex) at a ratio of 1:12 (w / v) at 40℃ for 2 hours. The extract was concentrated, the pH was adjusted to 7.5, and hydrolyzed at 50℃ with 1.5% complex protease (trypsin:flavor enzyme = 1:2) for 4 hours. The enzyme was inactivated, centrifuged, and spray-dried. Acidic pretreatment alters the higher-order structure of the protein, exposing enzyme cleavage sites; complex enzymatic hydrolysis controls the extraction of the target peptide fragment.

[0030] Black bean husk preparation method: Boil the black bean husk with a co-extract of pine needles and mulberry leaves.

[0031] Mixing is based on intermolecular forces, achieving spontaneous and uniform microscopic composite formation through precise feeding sequence and energy input. Specifically, a three-dimensional multi-directional motion mixer is used, and the mixing sequence and time include:

[0032] (1) Mix the first composite powder, the second composite powder, the third composite powder, and the fourth composite powder for 15 minutes each;

[0033] (2) Mix walnut kernel powder and black bean skin powder separately for 5 minutes each;

[0034] (3) Mix the powder from steps (1) and (2) with other components such as black sesame ultrafine powder, black bean peptide powder, and inulin, and stir for 15 minutes.

[0035] (4) Add anti-caking agent and filler and mix for 5 minutes.

[0036] After mixing, online near-infrared monitoring was used, and the uniformity RSD was <2.0%.

[0037] Furthermore, the preparation method of the black sesame powder includes: soaking black sesame seeds in edible rice vinegar containing 3-7% ginger juice for 0.5-1.5 hours, boiling for 5-15 minutes, steaming for 20-40 minutes, drying, and then crushing to a particle size D90≤50μm.

[0038] Furthermore, the preparation method of the black bean peptide powder includes: pretreating the black bean powder with an acidic solution of pH 5.0, and then hydrolyzing it with a complex protease at pH 7.5 and 50°C, with the degree of hydrolysis controlled at 15-20%.

[0039] Furthermore, the mixing in step e) is an interfacial self-assembly mixing, with a mixing time of 40–60 minutes and a mixing uniformity of ≥98%.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] 1. Using extracts rich in active ingredients from Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata as the reaction medium, and under precise temperature control of 45–50℃, the introduced raw materials such as Nostoc commune, black mulberry, and wolfberry not only undergo component dissolution and adsorption, but also drive the following two clearly directional transformations: First, covalent modification at the molecular chemical level: namely, the Maillard-polyphenol capture reaction, generating small molecule active compounds with new structures, which directly act on the hair follicle melanin synthesis process through molecular scaffolds and signal switches, strengthening the synthesis machinery and improving the efficiency and stability of melanin synthesis; Second, self-assembly at the supramolecular physical level: namely, the self-assembly of polyphenols, polysaccharides, and proteins, forming functionally customized composite nanocarriers, which directly act on the hair follicle growth cycle and stem cells through gut-hair follicle axis regulation, awakening the source of regeneration and fundamentally promoting healthy hair regeneration.

[0042] 2. By combining kelp and Alpinia oxyphylla, a trace element biotransformation and kidney-warming signaling system is constructed. Kelp is rich in iodine, zinc, and fucoidan, while Alpinia oxyphylla is pungent and warm, containing volatile oils. After being boiled together, kelp and Alpinia oxyphylla are stir-fried together with loess. In this process, the pungent and warm components of Alpinia oxyphylla penetrate into kelp, while the mineral heat medium of loess transforms trace elements such as calcium, iron, zinc, and iodine in kelp into more easily absorbed organic mineral salts, and stimulates the kidney-warming components of Alpinia oxyphylla. Together, they produce a synergistic effect of "promoting transformation through warmth and mineral transformation," targeting gray hair related to kidney essence deficiency.

[0043] 3. By combining Ziziphus jujuba seed and Cistanche deserticola, a neuroendocrine homeostasis regulation system is constructed. Ziziphus jujuba seed and Cistanche deserticola are co-soaked in baijiu (Chinese white liquor) to synergistically extract the sedative sine and the kidney-warming echinacoside. Subsequent loess roasting serves two purposes: firstly, under a uniform heat field, the mixed active ingredients enriched on the surface of the medicinal materials are thermally modified; the mineral surface of loess can catalyze the release of melanoidins, which have strong antioxidant properties and regulate intestinal flora. Secondly, during loess roasting, the high temperature causes rapid evaporation of moisture from the medicinal materials and cell structure rupture. Simultaneously, loess particles embed into the surface of the medicinal materials, forming a loose and porous new structure. This structure increases the dissolution rate of components, making it easier to extract Ziziphus jujuba seed saponins and Cistanche deserticola phenylethanoid glycosides with water, resulting in a physical synergistic effect. This achieves a deep coupling of sedative and kidney-warming effects, aiming to regulate the function of the hypothalamus-pituitary-adrenal (HPA) axis, which is closely related to hair health, and improve microcirculatory disorders in hair follicles caused by stress and sleep disturbances.

[0044] 4. The hair-darkening dietary composition of the present invention is a composite product prepared by synergistically combining Ge Xian Mi-Black Mulberry-Goji Berry Compound Powder, Huang Jing-Huang Qi-Dang Gui-Chen Pi Compound Powder, Kun Bu-Yi Zhi Ren Compound Powder, and Suan Zao Ren-Rou Cong Rong Compound Powder with nutritional fortification components (such as vinegar-ginger steamed black sesame ultrafine powder, chrysanthemum and hawthorn boiled walnut kernel powder, black bean peptide powder, etc.) processed with specific structure-activity preservation technology. It aims to solve the problem of non-pathological gray hair caused by nutritional signal transduction disorders, oxidative stress and systemic dysfunction from multiple dimensions such as anti-oxidation, system regulation, trace element supplementation and neuro-endocrine regulation, and improve the hair-darkening effect and efficiency. Attached Figure Description

[0045] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0046] Figure 1 Before and after comparison of the hair-darkening formula used in Example 1;

[0047] Figure 2 Before and after comparison of using the hair-darkening formula of Example 2;

[0048] Figure 3 Before and after comparison of using the hair-darkening formula of Example 3;

[0049] Figure 4 : Figure 1 A magnified view of a portion of the image;

[0050] Figure 5 : Figure 4 Image showing the hair separated from the scalp. Detailed Implementation

[0051] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0052] Unless otherwise specified, the reagents, instruments, equipment, preparation methods, or processes described in the following embodiments are all commonly used techniques in the field.

[0053] Examples 1-3

[0054] A hair-darkening dietary composition, formulated as shown in Table 1, comprises the following components by weight percentage:

[0055] Table 1 Formulations of Examples 1-3

[0056]

[0057]

[0058] In Table 1, the antagonist is silicon dioxide and the filler is maltodextrin.

[0059] In Example 1, the mass ratio of Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel in the first compound powder is 1:0.3:0.3:0.1; the mass ratio of Nostoc commune, black mulberry, and wolfberry in the second compound powder is 1:3:0.5; the mass ratio of kelp and Alpinia oxyphylla in the third compound powder is 1:0.8; and the mass ratio of Ziziphus jujuba var. spinosa and Cistanche deserticola in the fourth compound powder is 1:0.4.

[0060] In Example 2, the mass ratio of Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel in the first compound powder is 1:0.4:0.6:0.3; the mass ratio of Nostoc commune, black mulberry, and wolfberry in the second compound powder is 1:4:0.8; the mass ratio of kelp and Alpinia oxyphylla in the third compound powder is 1:1.2; and the mass ratio of Ziziphus jujuba var. spinosa and Cistanche deserticola in the fourth compound powder is 1:0.7.

[0061] In Example 3, the mass ratio of Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel in the first compound powder is 1:0.35:0.4:0.2; the mass ratio of Nostoc commune, black mulberry, and wolfberry in the second compound powder is 1:3.5:0.6; the mass ratio of kelp and Alpinia oxyphylla in the third compound powder is 1:1; and the mass ratio of Ziziphus jujuba var. spinosa and Cistanche deserticola in the fourth compound powder is 1:0.6.

[0062] Example 4

[0063] The preparation method of the hair-darkening dietary composition in Examples 1-3 includes the following steps:

[0064] a) Preparation of the first compound powder: Astragalus membranaceus, Angelica sinensis, and tangerine peel are mixed with water, boiled and maintained at a gentle boil for 5 minutes, Polygonatum sibiricum is added, and heating is stopped immediately after boiling again. The mixture is soaked together for 2 hours, and solid-liquid separation is performed to obtain solid and extract. The solid is dried and then pulverized together to obtain the first compound powder.

[0065] b) Preparation of the second composite powder: Ge Xian Mi, black mulberry and wolfberry are put into the extract solution at a temperature maintained at 45°C and soaked together for 1.5 hours. After drying, they are pulverized together to obtain the second composite powder.

[0066] c) Preparation of the third compound powder: Kelp and Alpinia oxyphylla are mixed, boiled together with water, then removed and stir-fried together with calcined loess. The loess is sieved out, cooled and then pulverized together.

[0067] d) Preparation of the fourth compound powder: Soak jujube seed and cistanche in white wine, then take them out and stir-fry them together with calcined loess. After sifting out the loess, cool them and grind them together.

[0068] e) Mix the first composite powder, the second composite powder, the third composite powder, and the fourth composite powder with black sesame powder, black bean peptide powder, walnut kernel powder, Ganoderma lucidum spore powder, black bean husk, inulin, black garlic extract, yam powder, Rehmannia glutinosa powder, mulberry leaf powder, microencapsulated B vitamins, anti-caking agent, and filler to obtain the final product.

[0069] Specifically, in step c), the co-cooking time is 0.5–1.5 hours, and the stir-frying is carried out at 100–120°C for 15–25 minutes; in step d), the alcohol content of the liquor is 40–60 degrees, the soaking time is 48–96 hours, the stir-frying temperature is 110–130°C, and the stir-frying time is 10–20 minutes; in step e), the mixing is an interfacial self-assembly mixing, the mixing time is 40–60 minutes, and the mixing uniformity is ≥98%.

[0070] The preparation method of black sesame powder includes: soaking black sesame seeds in edible rice vinegar containing 3-7% ginger juice for 0.5-1.5 hours, boiling for 5-15 minutes, steaming for 20-40 minutes, drying and crushing to a particle size D90≤50μm.

[0071] The preparation method of black bean peptide powder includes: black bean powder is pretreated with an acidic solution with a pH of 5.0, and then hydrolyzed with a complex protease at a pH of 7.5 and a temperature of 50°C, with the degree of hydrolysis controlled at 15-20%.

[0072] The preparation method of walnut kernel and lotus seed powder includes: simmering a mixture of walnut kernels and lotus seeds in a decoction of chrysanthemum and hawthorn for 10-20 minutes, then removing, drying, and pulverizing.

[0073] Comparative Example 1

[0074] Five-grain powder control group: Commercially available ordinary five-grain nutritional powder, main ingredients: black sesame 25%, black beans 25%, black rice 20%, walnuts 15%, yam 15%, representing the conventional approach of supplementing nutrition with black foods.

[0075] Comparative Example 2

[0076] The first composite powder in this invention is replaced with an equal amount of physically mixed powder of kudzu root, mulberry, and wolfberry without synergistic processing, while the processes for the remaining components remain unchanged.

[0077] Comparative Example 3

[0078] In this invention, the second composite powder is replaced with an equal amount of physically mixed powder of Astragalus membranaceus, Angelica sinensis, Citrus reticulata peel, and Polygonatum odoratum without synergistic processing, while the processes for the remaining components remain unchanged.

[0079] Comparative Example 4

[0080] The third composite powder in this invention is replaced with an equal amount of physically mixed kelp and Alpinia oxyphylla without synergistic processing, while the processes for the remaining components remain unchanged.

[0081] Comparative Example 5

[0082] The third composite powder in this invention is replaced with an equal amount of physically mixed powder of jujube seed and cistanche without synergistic processing, while the processes of the other components remain unchanged.

[0083] Example 1: Determination of Antioxidant Synergistic Index (SI)

[0084] The oxygen radical absorption capacity (ORAC value) of each single ingredient extract, its physical mixture, and the first, second, third, and fourth composite powders prepared by the synergistic process of this invention were measured respectively. SI = (measured ORAC of composite powder) / (sum of predicted ORAC of single ingredient powders) was calculated.

[0085] The results of the antioxidant synergy index (SI) measurement are shown in the table.

[0086] Table 2. Results of Antioxidant Synergistic Index (SI) Measurement

[0087] Element Synergy Index (SI) First composite powder Astragalus, Angelica sinensis, Citrus reticulata peel, Polygonatum sibiricum 1.41 Second composite powder Ge Xian Mi (a type of herb), black mulberry, and goji berries 1.31 Third compound powder Kelp, Alpinia officinarum 1.28 Fourth composite powder Sour jujube seed, Cistanche deserticola 1.33

[0088] As shown in Table 2, in the first compound powder, Astragalus membranaceus, Angelica sinensis, Citrus reticulata peel and Polygonatum sibiricum work synergistically to significantly enhance the adsorption effect of the carrier; in the second compound powder, Nostoc commune, black mulberry and wolfberry work synergistically to improve antioxidant properties; in the third compound powder, Laminaria japonica and Alpinia oxyphylla work synergistically to generate new activity through co-frying process; in the fourth compound powder, Ziziphus jujuba var. spinosa and Cistanche deserticola work synergistically to enhance the modification effect through co-processing with wine and soil.

[0089] Example 2: Effect of the third composite powder on the speciation of trace elements

[0090] Using an in vitro simulated gastrointestinal digestion model and ultrafiltration-atomic absorption combined method, the dissolution rate of zinc (Zn) and the proportion of organic complex in soluble Zn in the kelp-Alpinia oxyphylla composite powder prepared by the water-soil co-frying process of this invention and a simple mixture of the two were compared.

[0091] The results show that the Zn dissolution rate of the third composite powder of the present invention is increased by 32%, and the proportion of organically complexed Zn increases from 45% to 68% in the mixed powder. This further demonstrates that the co-processing of water and soil significantly improves the bioavailability and potential absorption forms of trace elements.

[0092] Example 3: Synergistic Regulation of HPA Axis-Related Indicators by the Fourth Composite Powder

[0093] A chronic restraint stress rat model was established. Groups included: blank control group, model group, Ziziphus jujuba seed extract group, Cistanche deserticola extract group, physical mixture group, and the fourth compound powder group of this invention. After 4 weeks of intervention, serum corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels and hypothalamic CRH mRNA expression were measured.

[0094] The results showed that the fourth compound powder group of the present invention was significantly better than each single-flavor group and the physical mixing group in reducing the expression of CORT, ACTH and CRH mRNA (p<0.05), which confirmed that the co-processing of wine and soil produced a unique neuro-endocrine regulatory synergistic effect.

[0095] Example 4: Comprehensive Performance Evaluation

[0096] The in vitro tyrosinase activation rate, the in vitro hair follicle melanin synthesis promotion rate, and the number of new black hairs after 8 weeks of use in mouse models were tested in Examples 1-3 and Comparative Examples 1-5, respectively. The results are shown in Table 3.

[0097] Table 3 Comprehensive Performance Evaluation Table

[0098]

[0099]

[0100] As shown in Table 3, the in vitro tyrosinase activation rate, in vitro hair follicle melanin synthesis promotion rate, and new black hair count in mouse models of Examples 1-3 were significantly better than those of Comparative Examples 1-5.

[0101] Modern medical research indicates that gray hair is caused by decreased tyrosinase activity, which prevents melanocytes from properly dividing melanin, thus leading to gray hair. This invention activates tyrosinase activity, increasing the melanin synthesis rate and thereby promoting hair darkening.

[0102] Example 4: Hair darkening and hair regrowth

[0103] 360 individuals aged 30-55 with premature gray hair were selected and divided into three groups. The first group used the hair-darkening formula from Example 1, the second group used the hair-darkening formula from Example 2, and the third group used the hair-darkening formula from Example 3. The dosage was 15 grams twice daily on an empty stomach, morning and evening, brewed with warm water, for 6 consecutive weeks.

[0104] Table 4. Number of new black hairs and rate of reduction in gray hair.

[0105] Average number of new black hairs (roots / cm) Average rate of reduction in gray hair (%) Example 1 28.5 41.8 Example 2 30.5 52.1 Example 3 36.7 61.3

[0106] Figure 1These are before-and-after comparison images of using the hair-darkening formula from Example 1. Figure 2 These are before-and-after comparison images of using the hair-darkening formula from Example 2. Figure 3 The images show a before-and-after comparison of the hair-darkening formula used in Example 3. The left side shows the hair before use, and the right side shows the hair after 6 weeks of use. It can be seen that after using the hair-darkening formula of the present invention for 6 weeks, the number of newly grown black hairs is significantly higher than the number of black hairs before 6 weeks.

[0107] Figure 4 To use a hair analyzer and hair mirror to Figure 1 The image on the right, magnified 50 times, clearly shows the enhancement of melanocytes from the hair root, with gray hair starting to turn black from the root, and new black hair growing next to the gray hair.

[0108] Figure 5 for Figure 4 The image shows the hair separated from the scalp, revealing that the roots of the white hair are new black hairs.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A hair-darkening dietary composition, characterized in that, It includes the following components by weight percentage: 15-20% of the first composite powder, 12-18% of the second composite powder, 1-6% of the third composite powder, and 2-8% of the fourth composite powder; The first compound powder includes Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel; the second compound powder includes Nostoc commune, black mulberry, and wolfberry; the third compound powder includes Laminaria japonica and Alpinia oxyphylla; and the fourth compound powder includes Ziziphus jujuba var. spinosa and Cistanche deserticola.

2. The hair-darkening dietary composition according to claim 1, characterized in that, In the first compound powder, the mass ratio of Polygonatum sibiricum, Astragalus membranaceus, Angelica sinensis, and Citrus reticulata peel is 1:0.3-0.4:0.3-0.6:0.1-0.3; In the second compound powder, the mass ratio of Nostoc commune, black mulberry, and wolfberry is 1:3-4:0.5-0.8; In the third composite powder, the mass ratio of kelp to Alpinia oxyphylla is 1:0.8-1.2; In the fourth composite powder, the mass ratio of jujube seed to cistanche is 1:0.4-0.

7.

3. The hair-darkening dietary composition according to claim 1 or 2, characterized in that, Also includes: Black sesame powder 14-16%, black bean peptide powder 5-6%, walnut kernel and lotus seed powder 3-5%, Ganoderma lucidum spore powder 2-3%, black bean husk 2-3%, inulin 2-3%, black garlic extract 2-3%, yam powder 1-2%, prepared rehmannia powder 1-2%, mulberry leaf powder 1-1.5%, microencapsulated B vitamins 0.5-1.0%, anti-caking agent 0.5-1.0%, and filler to 100%.

4. The hair-darkening dietary composition according to claim 3, characterized in that, The preparation method of the walnut kernel and lotus seed powder includes: simmering a mixture of walnut kernels and lotus seeds in a decoction of chrysanthemum and hawthorn for 10-20 minutes, then removing, drying, and pulverizing.

5. A method for preparing the hair-darkening dietary composition according to any one of claims 1-4, characterized in that, Includes the following steps: a) Preparation of the first compound powder: Astragalus membranaceus, Angelica sinensis, and tangerine peel are mixed with water, boiled and maintained at a gentle boil for 5 minutes, Polygonatum sibiricum is added, and heating is stopped immediately after boiling again. The mixture is soaked together for 2 hours, and solid-liquid separation is performed to obtain solid and extract. The solid is dried and then pulverized together to obtain the first compound powder. b) Preparation of the second composite powder: Ge Xian Mi, black mulberry, and wolfberry are put into the extract solution at a temperature maintained at 45-50℃, soaked together at a constant temperature for 1.5 hours, dried and then pulverized together to obtain the second composite powder; c) Preparation of the third compound powder: Kelp and Alpinia oxyphylla are mixed, boiled together with water, then removed and stir-fried together with calcined loess. The loess is sieved out, cooled and then pulverized together. d) Preparation of the fourth compound powder: Soak jujube seed and cistanche in white wine, then take them out and stir-fry them together with calcined loess. After sifting out the loess, cool them and grind them together. e) Mix the first composite powder, the second composite powder, the third composite powder, and the fourth composite powder with black sesame powder, black bean peptide powder, walnut kernel powder, Ganoderma lucidum spore powder, black bean husk, inulin, black garlic extract, yam powder, Rehmannia glutinosa powder, mulberry leaf powder, microencapsulated B vitamins, anti-caking agent, and filler to obtain the final product.

6. The method for preparing the hair-darkening dietary composition according to claim 5, characterized in that, In step c), the cooking time is 0.5 to 1.5 hours, and the stir-frying is carried out at 100 to 120°C for 15 to 25 minutes.

7. The method for preparing the hair-darkening dietary composition according to claim 5, characterized in that, In step d), the alcohol content of the liquor is 40-60 degrees, the soaking time is 48-96 hours, the stir-frying temperature is 110-130℃, and the stir-frying time is 10-20 minutes.

8. The method for preparing the hair-darkening dietary composition according to claim 5, characterized in that, The preparation method of the black sesame powder includes: soaking black sesame seeds in edible rice vinegar containing 3-7% ginger juice for 0.5-1.5 hours, boiling for 5-15 minutes, steaming for 20-40 minutes, drying, and then crushing to a particle size D90≤50μm.

9. The method for preparing the hair-darkening dietary composition according to claim 5, characterized in that, The preparation method of the black bean peptide powder includes: black bean powder is pretreated with an acidic solution with a pH of 5.0, and then hydrolyzed with a complex protease at a pH of 7.5 and a temperature of 50°C, with the degree of hydrolysis controlled at 15-20%.

10. The method for preparing the hair-darkening dietary composition according to claim 5, characterized in that, The mixing in step e) is an interfacial self-assembly mixing, with a mixing time of 40–60 minutes and a mixing uniformity of ≥98%.

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