Composition with instant red fading effect and application thereof

Abstract

The invention provides a composition with an instant red fading effect and application of the composition. The composition comprises a pinus densiflora extract, a radix gentianae extract and inositol choline glycerophosphate. The Pinus densiflora extract, the Gentiana scabra Bunge extract and the glycerophosphoinositide choline salt have a synergistic effect, and are combined for compatibility to inhibit release of inflammatory factors, so that vasodilatation and redness signals caused by inflammation are blocked from the source, and the skin barrier is immediately faded, relieved and repaired.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition with immediate redness-reducing effect and its application. Background Technology

[0002] External stimuli, allergic reactions, and environmental changes can all cause skin redness. In recent years, with the increase in the number of people with sensitive skin, the cosmetics industry has an increasingly urgent need for products with immediate redness-reducing and soothing effects.

[0003] CN118766816A discloses a composition with immediate redness-reducing effect and its application, wherein the composition comprises an extract of *Ipomoea quamoclit* peel and an extract of *Magnolia denudata* flower; the mass ratio of the extract of *Ipomoea quamoclit* peel and the extract of *Magnolia denudata* flower is (1-8):3. The composition exhibits significant immediate redness-reducing effect, with a 61.72% decrease in skin redness difference (a-value) and a 12.73% decrease in overall blood flow density 60 minutes after repair.

[0004] CN118986766A discloses an acne-reducing and redness-reducing composition, its application, and an acne-reducing essence. The composition comprises the following components in parts by weight: 1 part of asiaticoside-II, 0.67-70 parts of supramolecularly encapsulated salicylic acid, and 0.17-60 parts of a traditional Chinese medicine plant complex, including extracts of Coptis chinensis, Scutellaria baicalensis, Lonicera japonica, Salvia miltiorrhiza, Asparagus cochinchinensis, Sophora flavescens, Salix babylonica bark, and Phellodendron amurense bark. The composition achieves gentle yet effective acne-reducing, redness-soothing, and inflammatory cell-inhibiting effects.

[0005] CN119345056A discloses an instant redness-reducing composition and its application, a cosmetic composition and its preparation method, wherein the composition includes magnolin and fibronectin. It has high safety and can quickly relieve and eliminate skin redness.

[0006] Some existing products are slow to take effect and cannot meet the need for immediate redness reduction; some fast-acting products often have short-lasting effects or rely on high concentrations of ingredients. Therefore, there is still a need to provide highly effective cosmetic compositions for immediate redness reduction, focusing on inhibiting inflammatory factors, repairing the skin barrier, and reducing neurovascular reactivity. Summary of the Invention

[0007] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition with immediate redness-reducing effect and its application. The composition of this invention can block the vasodilation and redness signals caused by inflammation at the source, and immediately reduce redness, soothe and repair the skin barrier.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a composition having an immediate redness-reducing effect, the composition comprising red pine extract, gentian extract and glycerophosphate inositol choline salt.

[0010] In this invention, the extracts of red pine, gentian, and glycerophosphate inositol choline salt have a synergistic effect. The combined formulation inhibits the release of inflammatory factors, with histamine release inhibition rate of 46.09-70.07%, TNF-α expression inhibition rate of 46.46-76.94%, and skin erythema inhibition rate of 25.87-49.32%. It blocks the vasodilation and redness signals caused by inflammation at the source, and immediately reduces redness, soothes, and repairs the skin barrier.

[0011] Preferably, the composition comprises, by weight, 0.1-5 parts of red pine extract, 0.5-8 parts of gentian extract, and 0.01-2 parts of glycerophosphate inositol choline salt.

[0012] The weight parts of the red pine extract can be 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0013] The gentian extract can be present in the following weight proportions: 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, or 8 parts.

[0014] The weight parts of the glycerophosphoinositol choline salt can be 0.01 parts, 0.05 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 1 part, 1.3 parts, 1.5 parts, 1.8 parts, or 2 parts, etc.

[0015] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0016] Preferably, the composition comprises, by weight, 1-3 parts of red pine extract, 2-5 parts of gentian extract, and 0.05-0.5 parts of glycerophosphate inositol choline salt.

[0017] The weight parts of the red pine extract can be 1 part, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, or 3 parts, etc.

[0018] The gentian extract can be present in parts by weight of 2, 2.2, 2.5, 2.7, 3, 3.3, 3.5, 3.8, 4, 4.5, or 5 parts, etc.

[0019] The weight parts of the glycerophosphoinositol choline salt can be 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, or 0.5 parts, etc.

[0020] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0021] The composition of the present invention has better anti-inflammatory and redness-reducing effects when the ratio is within the above range.

[0022] Preferably, the red pine extract is prepared by a method comprising the following steps:

[0023] The raw material of red pine, enzyme and water are mixed and subjected to enzymatic hydrolysis. Solid-liquid separation is performed, the precipitate is taken for alcohol extraction, ultrasonic treatment is performed, and the liquid is collected to obtain the red pine extract.

[0024] The red pine extract prepared by the specific method of this invention, when combined with gentian extract and glycerophosphate inositol choline salt, has better anti-inflammatory and redness-reducing effects.

[0025] Preferably, the mass ratio of the red pine raw material, enzyme and water is 1:(0.01-0.1):(5-10).

[0026] The specific point values ​​in (0.01-0.1) can be 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09 or 0.1, etc.

[0027] The specific point values ​​in (5-10) can be 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5 or 10, etc.

[0028] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0029] Preferably, the temperature of the enzymatic hydrolysis reaction is 35-45℃, for example, 35℃, 36℃, 37℃, 38℃, 39℃, 40℃, 41℃, 42℃, 43℃, 44℃ or 45℃, etc.; the time is 1-3 h, for example, 1 h, 1.2 h, 1.5 h, 1.7 h, 2 h, 2.3 h, 2.5 h, 2.8 h or 3 h, etc.

[0030] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0031] Preferably, the enzyme comprises any one or a combination of at least two of cellulase, β-glucosidase, or laccase.

[0032] Preferably, the enzymes include cellulase, β-glucosidase, and laccase.

[0033] This invention preferably uses a combination of cellulase, β-glucosidase and laccase to process red pine raw materials. The three enzymes have a synergistic effect, which can fully destroy the macromolecular components in red pine raw materials, facilitate the dissolution of active substances, and enhance the anti-inflammatory and redness-reducing effects of the composition.

[0034] Preferably, the mass ratio of cellulase, β-glucosidase and laccase is (1-5):(1-5):(1-5).

[0035] The specific point values ​​in the first (1-5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0036] The specific point values ​​in the second (1-5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0037] The specific point values ​​in the third (1-5) can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5, etc.

[0038] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0039] Preferably, the reagent for the alcohol extraction is a 65-90 vol% aqueous ethanol solution, such as 65 vol%, 67 vol%, 70 vol%, 73 vol%, 75 vol%, 78 vol%, 80 vol%, 85 vol%, or 90 vol%.

[0040] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0041] Preferably, the ratio of the red pine raw material to the ethanol aqueous solution is 1 g:(3-4) mL.

[0042] The specific point values ​​in (3-4) can be 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9 or 4, etc.

[0043] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0044] Preferably, the power of the ultrasound is 100-300 W, for example, 100 W, 120 W, 150 W, 170 W, 200 W, 230 W, 250 W, 280 W or 300 W; the duration is 20-60 min, for example, 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min.

[0045] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0046] Preferably, the collected liquid further includes a concentration and drying step.

[0047] Preferably, the gentian extract is prepared by a method comprising the following steps:

[0048] The gentian raw material was subjected to alcohol extraction, solid-liquid separation, and the liquid was collected to obtain the gentian extract.

[0049] Preferably, the reagent for alcohol extraction is an aqueous solution of 50-90 vol% alcohol.

[0050] The specific point values ​​in the 50-90 vol% range can be 50 vol%, 55 vol%, 60 vol%, 65 vol%, 70 vol%, 75 vol%, 80 vol%, 85 vol%, or 90 vol%, etc.

[0051] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0052] Preferably, the alcohol includes any one or a combination of at least two of ethanol, methanol, 1,2-hexanediol, 1,2-pentanediol, ethylene glycol, propylene glycol, butanediol, glycerol, polyethylene glycol, or sorbitol.

[0053] Preferably, the ratio of gentian raw material to extraction reagent is 1 g:(5-15) mL.

[0054] The specific point values ​​in (5-15) can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15, etc.

[0055] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0056] Preferably, the alcohol extraction time is 1-5 h, for example, it can be 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h or 5 h, etc.

[0057] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0058] The methods for preparing gentian extract involved in this invention include, but are not limited to, the methods described above. Gentian extracts prepared by methods commonly used in the art are also applicable to this invention.

[0059] In a second aspect, the present invention provides the use of the composition as described in the first aspect in the preparation of cosmetics.

[0060] Preferably, the cosmetic includes any one of toner, lotion, cream, serum, spray, or mask.

[0061] Preferably, the composition has a mass percentage content of 0.01-15% in the cosmetic, such as 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 5%, 8%, 10%, 13%, or 15%.

[0062] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0063] Preferably, the cosmetic also includes an excipient matrix, which includes any one or a combination of at least two of antioxidants, moisturizers, emollients, emulsifiers, thickeners, solubilizers, chelating agents, pH adjusters, solvents, or preservatives.

[0064] Thirdly, the present invention provides a toner with an immediate redness-reducing effect, wherein the toner comprises, by weight, 0.01-15 parts of the composition described in the first aspect, 1-5 parts of the redness-reducing agent, and 50-95 parts of the excipient matrix.

[0065] The composition may be in parts by weight of 0.01, 0.05, 0.1, 0.5, 1, 2, 5, 8, 10, 13, or 15 parts, etc.

[0066] The redness-reducing agent can be present in parts by weight of 1, 1.2, 1.5, 1.7, 2, 2.3, 2.5, 2.8, 3, 3.5, 4, 4.5, or 5 parts, etc.

[0067] The weight percentage of the auxiliary material matrix can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, or 95 parts, etc.

[0068] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0069] The toner provided by this invention can inhibit the release of inflammatory factors, block the vasodilation and redness signals caused by inflammation at the source, and immediately reduce redness, soothe and repair the skin barrier.

[0070] Preferably, the redness-reducing agent comprises any one or a combination of at least two of serine, bisabolol, or tranexamic acid.

[0071] Preferably, the redness-reducing agent includes serine, bisabolol, and tranexamic acid.

[0072] The present invention preferably uses a combination of serine, bisabolol and tranexamic acid as an adjuvant to help enhance the anti-inflammatory and redness-reducing effects of the composition.

[0073] Preferably, the mass ratio of serine, bisabolol and tranexamic acid is (1-10):(1-10):(1-10).

[0074] The specific point values ​​in the first (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0075] The specific point values ​​in the second (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0076] The specific point values ​​in the third (1-10) can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, etc.

[0077] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0078] Compared with the prior art, the present invention has the following beneficial effects:

[0079] In this invention, the extracts of red pine, gentian, and glycerophosphate inositol choline salt have a synergistic effect. The combined formulation inhibits the release of inflammatory factors, with histamine release inhibition rate of 46.09-70.07%, TNF-α expression inhibition rate of 46.46-76.94%, and skin erythema inhibition rate of 25.87-49.32%. It blocks the vasodilation and redness signals caused by inflammation at the source, and immediately reduces redness, soothes, and repairs the skin barrier. Detailed Implementation

[0080] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0081] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0082] The sources of materials used in the following specific embodiments are as follows:

[0083] By weight, 5 parts of 1,3-propanediol, 0.05 parts of xanthan gum, 5 parts of glycerin, 0.05 parts of disodium EDTA, 0.5 parts of 1,2-hexanediol, 0.5 parts of p-hydroxyacetophenone and 88.9 parts of water were mixed evenly to prepare a cosmetic water excipient matrix.

[0084] Cellulase, β-glucosidase, and laccase were purchased from Novozymes (China) Biotechnology Co., Ltd.; glycerophosphoinositol choline salt was purchased from Sederma SAS.

[0085] Preparation Example 1

[0086] This preparation example provides a red pine extract, which is prepared by a method comprising the following steps:

[0087] Wash the buds of European red pine with water, dry them, and then pulverize them for later use. Add 10 g of European red pine buds, 0.2 g of cellulase, 0.2 g of β-glucosidase, and 0.1 g of laccase to 75 g of water, mix well, and perform enzymatic hydrolysis at 40°C for 2 h. Filter, collect the precipitate, add 35 mL of 75 vol% ethanol aqueous solution, and sonicate at 200 W for 40 min. Filter, collect the liquid, concentrate and dry it to obtain the red pine extract.

[0088] Preparation Example 2

[0089] This preparation example provides a red pine extract, which is prepared by a method comprising the following steps:

[0090] Wash the buds of European red pine with water, dry them, and then pulverize them for later use. Add 10 g of European red pine buds, 0.3 g of cellulase, 0.4 g of β-glucosidase, and 0.3 g of laccase to 100 g of water, mix well, and perform enzymatic hydrolysis at 35°C for 3 h. Filter, collect the precipitate, add 30 mL of 90 vol% ethanol aqueous solution, and sonicate at 300 W for 20 min. Filter, collect the liquid, concentrate and dry it to obtain the red pine extract.

[0091] Preparation Example 3

[0092] This preparation example provides a red pine extract, which is prepared by a method comprising the following steps:

[0093] Wash the buds of European red pine with water, dry them, and then pulverize them for later use. Add 10 g of European red pine buds, 0.04 g of cellulase, 0.03 g of β-glucosidase, and 0.03 g of laccase to 50 g of water, mix well, and perform enzymatic hydrolysis at 45°C for 1 h. Filter, collect the precipitate, add 40 mL of 65 vol% ethanol aqueous solution, and sonicate at 100 W for 60 min. Filter, collect the liquid, concentrate and dry it to obtain the red pine extract.

[0094] Preparation Example 4

[0095] This preparation example provides a gentian extract, which is prepared by a method comprising the following steps:

[0096] Gentian root was washed with water, dried, and pulverized for later use. 10 g of gentian root was mixed with 150 mL of 90 vol% ethanol aqueous solution and extracted for 1 h. The mixture was filtered, the liquid was collected, concentrated, and dried to obtain the gentian extract.

[0097] Preparation Example 5

[0098] This preparation example provides a gentian extract, which is prepared by a method comprising the following steps:

[0099] Gentian root was washed with water, dried, and pulverized for later use. 10 g of gentian root was mixed with 50 mL of 50% methanol aqueous solution and extracted for 5 h. The mixture was filtered, the liquid was collected, concentrated, and dried to obtain the gentian extract.

[0100] Preparation Example 6

[0101] This preparation example provides a red pine extract, which differs from Preparation Example 1 only in that: no cellulase was added, and its reduced amount was proportionally allocated to β-glucosidase and laccase, while the other steps remained unchanged.

[0102] Preparation Example 7

[0103] This preparation example provides a red pine extract, which differs from Preparation Example 1 only in that: β-glucosidase is not added, and its reduced amount is proportionally allocated to cellulase and laccase, while the other steps remain unchanged.

[0104] Preparation Example 8

[0105] This preparation example provides a red pine extract, which differs from Preparation Example 1 only in that: laccase is not added, and its reduced amount is proportionally allocated to cellulase and β-glucosidase, while the other steps remain unchanged.

[0106] Example 1

[0107] This embodiment provides a composition with immediate redness-reducing effect, which comprises the following components by weight: 2 parts of red pine extract provided in Preparation Example 1, 3 parts of gentian extract provided in Preparation Example 4, and 0.1 parts of glycerophosphate inositol choline salt. The preparation method is simply to mix the above raw materials evenly.

[0108] Example 2

[0109] This embodiment provides a composition with immediate redness-reducing effect, which comprises the following components by weight: 3 parts of red pine extract provided in Preparation Example 2, 2 parts of gentian extract provided in Preparation Example 5, and 0.5 parts of glycerophosphate inositol choline salt. The preparation method is simply to mix the above raw materials evenly.

[0110] Example 3

[0111] This embodiment provides a composition with immediate redness-reducing effect, which comprises the following components by weight: 1 part of red pine extract provided in Preparation Example 3, 5 parts of gentian extract provided in Preparation Example 4, and 0.05 parts of glycerophosphate inositol choline salt. The preparation method is simply to mix the above raw materials evenly.

[0112] Example 4

[0113] This embodiment provides a composition with immediate redness-reducing effect, comprising the following components by weight: 5 parts of red pine extract provided in Preparation Example 1, 0.5 parts of gentian extract provided in Preparation Example 4, and 2 parts of glycerophosphate inositol choline salt. The preparation method involves mixing the above raw materials evenly.

[0114] Example 5

[0115] This embodiment provides a composition with immediate redness-reducing effect, which comprises the following components by weight: 0.1 parts of red pine extract provided in Preparation Example 1, 8 parts of gentian extract provided in Preparation Example 4, and 0.01 parts of glycerophosphate inositol choline salt. The preparation method is simply to mix the above raw materials evenly.

[0116] Examples 6-8

[0117] This embodiment provides a composition with immediate redness-reducing effect. The only difference between this composition and Example 1 is that the "red pine extract provided in Preparation Example 1" is replaced with an equal amount of "red pine extract provided in Preparation Examples 6-8", while all other raw materials remain unchanged.

[0118] Comparative Example 1

[0119] This comparative example provides a composition with immediate redness-reducing effect, which differs from Example 1 only in that: red pine extract is not added, and its reduced amount is allocated to gentian extract and glycerophosphate inositol choline salt in proportion, while the remaining raw materials remain unchanged.

[0120] Comparative Example 2

[0121] This comparative example provides a composition with immediate redness-reducing effect, which differs from Example 1 only in that: gentian extract is not added, and its reduced amount is allocated proportionally to red pine extract and glycerophosphate inositol choline salt, while the remaining ingredients remain unchanged.

[0122] Comparative Example 3

[0123] This comparative example provides a composition with immediate redness-reducing effect, which differs from Example 1 only in that: glycerophosphate inositol choline salt is not added, and its reduced amount is proportionally allocated to the red pine extract and gentian extract, while the remaining raw materials remain unchanged.

[0124] Application Example 1

[0125] This application example provides a toner with an immediate redness-reducing effect, which comprises the following components by weight: 5 parts of the composition provided in Example 1, 1 part of serine, 1 part of bisabolol, 1 part of tranexamic acid and 70 parts of excipient matrix.

[0126] Application Example 2

[0127] This application example provides a toner with an immediate redness-reducing effect, which comprises the following components by weight: 4 parts of the composition provided in Example 2, 0.4 parts of serine, 0.3 parts of bisabolol, 0.3 parts of tranexamic acid, and 60 parts of excipient matrix.

[0128] Application Example 3

[0129] This application example provides a toner with an immediate redness-reducing effect, which comprises the following components by weight: 5.5 parts of the composition provided in Example 3, 2 parts of serine, 1.5 parts of bisabolol, 1.5 parts of tranexamic acid, and 80 parts of excipient matrix.

[0130] Application Example 4-8

[0131] This application example provides a toner with an immediate redness-reducing effect. The only difference between this application example and application example 1 is that the "composition provided in example 1" is replaced with an equal amount of the "composition provided in examples 4-8", while all other ingredients remain unchanged.

[0132] Application Example 9

[0133] This application example provides a toner with an immediate redness-reducing effect. The only difference between this toner and application example 1 is that serine is not added, and its reduced amount is proportionally allocated to bisabolol and tranexamic acid, while the other ingredients remain unchanged.

[0134] Application Example 10

[0135] This application example provides a toner with an immediate redness-reducing effect. The only difference between this toner and application example 1 is that bisabolol is not added, and its reduced amount is proportionally allocated to serine and tranexamic acid, while the other ingredients remain unchanged.

[0136] Application Example 11

[0137] This application example provides a toner with an immediate redness-reducing effect. The only difference between this toner and application example 1 is that tranexamic acid is not added, and its reduced amount is proportionally allocated to serine and bisabolol, while the other ingredients remain unchanged.

[0138] Comparative Application Examples 1-3

[0139] This comparative application example provides a toner with an immediate redness-reducing effect. The only difference between this and application example 1 is that the "composition provided in Example 1" is replaced with an equal amount of the "composition provided in Comparative Examples 1-3", while all other ingredients remain unchanged.

[0140] Test Example 1

[0141] The compositions provided in Examples 1-8 and Comparative Examples 1-3 were used to prepare a 10 g / mL stock solution with dimethyl sulfoxide (DMSO), which was then diluted to a 0.5 g / mL test sample solution with IMDM medium containing 10% fetal bovine serum. Human peripheral blood basophilic cells KU812 were seeded in 96-well plates with IMDM medium containing 10% fetal bovine serum at a cell density of 4 × 10⁶ cells / well. 5 Samples / well. Incubate at 37℃ in a 5% CO2 incubator for 10 min. Add 20 μL of test sample solution to each well. The model control group and blank control group each receive 20 μL of culture medium. Each group has three replicates. After incubation for 2 h, add 5 μL of 40 nM crotonol-12-tetradecanoate-13-acetate (PMA) and 5 μL of 1 μM calcium ion carrier (A23187) to the experimental and model groups. Add 10 μL of DMSO to the blank group.

[0142] After 2 hours of incubation, cells were centrifuged to separate them, and the cell supernatant was collected. The levels of histamine and TNF-α in the supernatant were measured using an ELISA kit. The results showed that the levels in the blank group were significantly lower than those in the model group, indicating successful modeling. Histamine release inhibition rate (%) = (histamine content in the model group - histamine content in the experimental group) / histamine content in the model group × 100%; TNF-α expression inhibition rate (%) = (TNF-α content in the model group - TNF-α content in the experimental group) / TNF-α content in the model group × 100%.

[0143] The test results are shown in Table 1: Examples 1-3 and Comparative Examples 1-3 show that *Pinus tabuliformis* extract, *Gentiana scabra* extract, and glycerophosphate inositol choline salt have a synergistic effect, inhibiting the expression and release of histamine and TNF-α, thus blocking vasodilation and redness signals caused by inflammation at their source. Examples 4-5 show that the ratio of *Pinus tabuliformis* extract, *Gentiana scabra* extract, and glycerophosphate inositol choline salt affects the inhibitory effect on inflammatory factors. Examples 6-8 show that cellulase, β-glucosidase, and laccase have a synergistic effect, effectively destroying the macromolecular components in the *Pinus tabuliformis* raw material, facilitating the dissolution of active substances, and enhancing the anti-inflammatory efficacy of the composition.

[0144] Table 1

[0145]

[0146] Test Example 2

[0147] One hundred and fifty participants, aged 18-50 years, were randomly divided into 15 groups of 10 each. The amount of erythema on the forearm of each participant was measured using a Mexameter MX18 erythema analyzer (Courage-Khazaka Electronic, Germany), and this amount was set as 0 (baseline). 0.02 mL of 3% sodium dodecyl sulfate (SDS) aqueous solution was precisely pipetted into the center of each patch applicator chamber and then applied to the designated area on the volunteer's forearm. The patch applicator was removed after 24 hours. The amount of erythema was measured 4 hours later and set as 100%. One day later, the same amount of the toner provided in Examples 1-11 or Comparative Examples 1-3 was applied to the experimental area on the forearm of each participant; the control group received an equal amount of the toner's excipient base. Four hours after application, the amount of skin erythema was measured to calculate the skin erythema inhibition rate. Skin erythema inhibition rate (%) = (Erythema amount in control group - Erythema amount in experimental group) / Erythema amount in control group × 100%.

[0148] The test results are shown in Table 2: Application Examples 1-3 and Comparative Application Examples 1-3 show that *Pinus tabuliformis* extract, *Gentiana scabra* extract, and glycerophosphate inositol choline salt have a synergistic effect, reducing the generation of redness signals and exerting an immediate redness-reducing effect. Application Examples 4-5 show that the content ratio of *Pinus tabuliformis* extract, *Gentiana scabra* extract, and glycerophosphate inositol choline salt affects the redness-reducing effect. Application Examples 6-8 show that cellulase, β-glucosidase, and laccase have a synergistic effect, effectively breaking down the macromolecular components in the *Pinus tabuliformis* raw material, facilitating the dissolution of active substances, and enhancing the redness-reducing effect of the composition. Application Examples 9-11 show that serine, bisabolol, and tranexamic acid have a synergistic effect, further enhancing the redness-reducing effect of the composition.

[0149] Table 2

[0150]

[0151] This invention illustrates a composition with immediate redness-reducing effect and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of the raw materials in the product, addition of auxiliary components, and selection of specific methods all fall within the protection and disclosure scope of this invention.

[0152] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0153] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A composition having an immediate redness-reducing effect, characterized in that, The composition comprises red pine extract, gentian extract and glycerophosphate inositol choline salt.

2. The composition according to claim 1, characterized in that, The composition comprises, by weight, 0.1-5 parts of red pine extract, 0.5-8 parts of gentian extract, and 0.01-2 parts of glycerophosphate inositol choline salt; Preferably, the composition comprises, by weight, 1-3 parts of red pine extract, 2-5 parts of gentian extract, and 0.05-0.5 parts of glycerophosphate inositol choline salt.

3. The composition according to claim 1 or 2, characterized in that, The red pine extract was prepared by a method comprising the following steps: The raw material of red pine, enzyme and water are mixed and subjected to enzymatic hydrolysis. Solid-liquid separation is performed, the precipitate is taken for alcohol extraction, ultrasonic treatment is performed, and the liquid is collected to obtain the red pine extract.

4. The composition according to claim 3, characterized in that, The mass ratio of the red pine raw material, enzyme and water is 1:(0.01-0.1):(5-10); Preferably, the enzymatic hydrolysis reaction is carried out at a temperature of 35-45°C for 1-3 hours. Preferably, the enzyme comprises any one or a combination of at least two of cellulase, β-glucosidase, or laccase.

5. The composition according to claim 3 or 4, characterized in that, The enzymes include cellulase, β-glucosidase, and laccase; Preferably, the mass ratio of cellulase, β-glucosidase and laccase is (1-5):(1-5):(1-5).

6. The composition according to any one of claims 3-5, characterized in that, The reagent used for alcohol extraction is a 65-90 vol% aqueous ethanol solution; Preferably, the ratio of the red pine raw material to the ethanol aqueous solution is 1 g:(3-4) mL; Preferably, the power of the ultrasound is 100-300 W and the duration is 20-60 min; Preferably, the collected liquid further includes a concentration and drying step.

7. The use of the composition according to any one of claims 1-6 in the preparation of cosmetics.

8. The application according to claim 7, characterized in that, The cosmetics include any one of the following: toner, lotion, cream, serum, spray, or mask; Preferably, the composition has a mass percentage content of 0.01-15% in the cosmetic. Preferably, the cosmetic also includes an excipient matrix, which includes any one or a combination of at least two of antioxidants, moisturizers, emollients, emulsifiers, thickeners, solubilizers, chelating agents, pH adjusters, solvents, or preservatives.

9. A toner with instant redness-reducing effect, characterized in that, The lotion comprises, by weight, 0.01-15 parts of the composition according to any one of claims 1-6, 1-5 parts of the redness-reducing agent, and 50-95 parts of the excipient matrix.

10. The lotion according to claim 9, characterized in that, The redness-reducing adjuvant includes any one or a combination of at least two of serine, bisabolol, or tranexamic acid. Preferably, the redness-reducing agent includes serine, bisabolol, and tranexamic acid; Preferably, the mass ratio of serine, bisabolol and tranexamic acid is (1-10):(1-10):(1-10).

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