Oil control composition as well as application and cosmetic thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses an oil control composition and application thereof and cosmetics, the oil control composition comprises the following components: bletilla striata root hydrolat, kombucha fermentation liquor and an oat kernel extract solution; the mass ratio of the rhizoma bletillae root hydrolat to the kombucha fermentation liquor to the oat kernel extract solution is (1-10): (1-5): (1-5). The three components are compounded for use, a remarkable synergistic effect is achieved, the 5alpha-reductase inhibition rate of the composition can be increased synergistically, the highest 5alpha-reductase inhibition rate reaches 28.18%, and the composition has an efficient oil control effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and discloses an oil-controlling composition and its uses, as well as cosmetics. Background Technology

[0002] Oily skin is essentially the physiological process of sebum secretion by sebaceous glands. Sebaceous glands, as appendages of the skin, are mainly distributed on the face, scalp, chest, and back. The sebum they secrete is composed of triglycerides, fatty acids, squalene, cholesterol esters, and other components. Under normal circumstances, it forms a sebum film, playing a vital role in moisturizing, locking in moisture, and protecting against external stimuli. The normal sebum secretion rate in healthy adults is approximately 1 mg / 10 cm³ every 3 hours. 2 When the secretion exceeds 1.5 mg / 10 cm 2 Excess sebum buildup on the skin's surface not only directly leads to oily, sticky skin but also triggers a series of problems: First, excess sebum mixes with dead skin cells and environmental dust to form plugs, clogging hair follicles and causing pores to appear enlarged. Second, sebum provides ample nutrition for lipophilic microorganisms such as Propionibacterium acnes and Malassezia, causing them to multiply abnormally and produce metabolic products that stimulate inflammation of the hair follicle sebaceous glands, leading to inflammatory acne and folliculitis. Long-term, recurring outbreaks can also leave acne marks and scars, seriously affecting skin health and appearance. Furthermore, excessive oil production accelerates makeup fading and causes skincare products to pill, reducing the stability of skincare and makeup effects. Therefore, preventing excessive oil production and maintaining healthy skin is increasingly valued.

[0003] Currently, there are various oil-control products on the market. Most traditional products rely on physical adsorption (such as kaolin, talc, and silica) or surface degreasing ingredients (such as soap base and sulfates). They can only temporarily remove the oil secreted on the skin surface through physical adsorption or chemical exfoliation, and cannot regulate the synthesis and secretion function of sebaceous glands from the source, which is a "treat the symptoms but not the root cause" approach. Some chemical oil-control ingredients (such as salicylic acid and azelaic acid) can dissolve oil and unclog pores, but they are highly irritating. Long-term use can easily damage the lipid structure of the skin barrier, leading to a decrease in the skin's ability to retain moisture, and thus causing "oily on the outside but dry on the inside," that is, a contradictory state in which there is excess oil on the skin surface and insufficient moisture content in the stratum corneum. This is especially unsuitable for people with sensitive skin or acne-prone skin whose skin barrier is already fragile. Furthermore, most oil-control products have a single effective ingredient and a single target, resulting in limited oil control dimensions and unsatisfactory oil control effects, failing to achieve efficient oil control from multiple dimensions.

[0004] Therefore, the technical problem to be solved by the present invention is: how to provide a novel oil-control composition with multi-dimensional and efficient oil-control effects to overcome the shortcomings of existing products. Summary of the Invention

[0005] The purpose of this invention is to provide an oil-controlling composition containing Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution. Combining these three components has a synergistic effect in increasing the oil-controlling effect of the composition.

[0006] In addition, the present invention also provides the use of the oil-controlling composition and cosmetics containing the oil-controlling composition.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] An oil-controlling composition comprising the following components: Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution; wherein the mass ratio of Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution is 1~10:1~5:1~5.

[0009] In this invention, the combination of three components—Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution—may have a synergistic effect in enhancing the oil-controlling effect of the composition. The mechanism may be as follows:

[0010] The polysaccharides in Bletilla striata root hydrosol can form a gentle protective film on the skin surface, reducing the outward penetration of sebum secreted by the sebaceous glands. At the same time, Bletilla striata root hydrosol has good moisturizing properties, which can relieve the compensatory oil production caused by dryness and indirectly help control oil.

[0011] Kombucha fermentation liquid can effectively inhibit Propionibacterium acnes in low amounts without disrupting other microbial communities around the skin. Propionibacterium acnes decomposes skin oil to produce free fatty acids, which stimulates the sebaceous glands to secrete excessive oil. Reducing the number of this bacterium can reduce this stimulation and indirectly control oil production.

[0012] Oat kernel extract solution can promote the repair and regeneration of skin stratum corneum cells, prevent the skin from compensating for dehydration by secreting more oil, thereby maintaining the skin's water-oil homeostasis and achieving a long-lasting oil-control effect.

[0013] The three components work synergistically to achieve multi-dimensional oil control through "moisturizing + antibacterial + repairing", resulting in highly effective oil control.

[0014] Preferably, the mass ratio of the Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution is 5:3:2.

[0015] Preferably, the Bletilla striata root hydrosol comprises 99 wt% Bletilla striata root water, 0.5 wt% p-hydroxyacetophenone and 0.5 wt% 1,2-hexanediol by mass percentage.

[0016] Preferably, the kombucha fermentation broth contains, by mass percentage, 0.05 wt% yeast / acetobacterium xylinum / black tea fermentation product, 40 wt% butylene glycol and 59.95 wt% water.

[0017] Preferably, the oat kernel extract solution comprises 0.55 wt% oat kernel extract and 99.45 wt% glycerol by weight percentage.

[0018] Furthermore, this invention discloses the use of the oil-controlling composition described above in the preparation of cosmetics.

[0019] Preferably, the cosmetic is an oil-controlling cosmetic.

[0020] Finally, this invention discloses a cosmetic product containing the oil-controlling composition described above.

[0021] Preferably, the content of the oil-control composition in the cosmetic is 5-15 wt%.

[0022] Preferably, the dosage form of the cosmetic is one of emulsion, aqueous solution, cream, or spray.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] The oil-controlling composition provided by this invention comprises three components: Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution. When these three components are used in combination, they have a significant synergistic effect, which can synergistically increase the 5α-reductase inhibition rate of the composition and achieve a highly efficient oil-controlling effect. Attached Figure Description

[0025] Figure 1 Diagram showing the severity of skin lesions;

[0026] Figure 2 VISIA-CR images of subject ① at D0, D14, and D28;

[0027] Figure 3 VISIA-CR images of subject ② at D0, D14, and D28;

[0028] Figure 4 VISIA-CR images of subject ③ at D0, D14, and D28;

[0029] Figure 5 VISIA-CR images of subject ④ at D0, D14, and D28;

[0030] Figure 6 VISIA-CR images of subject ⑤ at D0, D14, and D28;

[0031] Figure 7 VISIA-CR images of subject ⑥ at D0, D14, and D28. Detailed Implementation

[0032] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0033] Product Information:

[0034] Bletilla striata root hydrosol: Purchased from LanHai Biotechnology Co., Ltd., product name 611145 Bletilla striata root hydrosol, containing 99wt% Bletilla striata root water, 0.5wt% p-hydroxyacetophenone and 0.5wt% 1,2-hexanediol.

[0035] Kombucha fermentation broth: purchased from LanHai Biotechnology Co., Ltd., trade name 851001 Ferment BT, containing 0.05wt% yeast / Acetobacter xylinum / black tea fermentation product, 40wt% butylene glycol and 59.95wt% water.

[0036] Oat kernel extract solution: purchased from LanHai Biotechnology Co., Ltd., trade name 141031 Oat Biological Anti-inflammatory Compound, containing 0.55wt% oat kernel extract and 99.45wt% glycerin.

[0037] It should be noted that the technical solution of this application can be achieved by purchasing other commercially available raw materials, and is not limited to the aforementioned raw material suppliers.

[0038] The specific formulations of the oil-controlling compositions in each embodiment and comparative example are shown in Table 1;

[0039] Table 1. Composition Formulation Table (Unit: Parts by Mass)

[0040] Bletilla striata root hydrosol Kombucha fermentation liquid Oat kernel extract solution Example 1 5 3 2 Example 2 1 10 1 Example 3 5 1 5 Comparative Example 1 0 6 4 Comparative Example 2 7.14 0 2.86 Comparative Example 3 6.25 3.75 0 Comparative Example 4 10 0 0 Comparative Example 5 0 10 0 Comparative Example 6 0 0 10

[0041] The preparation methods of the oil-controlling compositions in each embodiment and comparative example are shown below:

[0042] The oil-controlling composition can be obtained by mixing the components in proportion and stirring evenly.

[0043] Performance testing

[0044] Chicken embryo chorionic allantoic membrane experiment

[0045] 1. Experimental Principle

[0046] This experiment utilizes the intact, clear, and transparent chorioallantoic membrane vascular system of mid-stage hatched chicken embryos. A certain amount of the test substance is directly exposed to the chorioallantoic membrane of the chicken embryo. After a period of time, changes in chorioallantoic membrane toxicity indicators (such as hemorrhage, coagulation, and angiolysis) are observed. These indicators reflect changes in the morphology, structure, color, and permeability of blood vessels and vascular networks, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. These indicators are then combined to obtain a score to assess the eye irritation of the test substance.

[0047] 2. Experimental Objective

[0048] The standard describes the ability of a test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos, and the procedures for evaluating the potential eye irritation factors of the substance being assessed.

[0049] 3. Vascular effect

[0050] (1) Bleeding: Blood flows out from the blood vessels and / or capillaries of the CAM. Bleeding can take many forms, such as cauliflower-like, smooth, diffuse gauze-like or punctate bleeding (due to the selective outflow of blood from different areas of the vascular membrane).

[0051] (2) Coagulation: Denaturation of intravascular and extravascular proteins, usually only seen in large and medium-sized blood vessels, excluding changes in capillaries. Thrombosis: Intravascular coagulation, caused by various reasons that interrupt blood flow within blood vessels, such as changes in vascular pressure or swelling of the vessel wall, manifests as dark-colored coagulation spots within the blood vessel. Extravascular coagulation: Can manifest as dark-colored coagulation spots outside the blood vessel; it can also manifest as turbidity (opaqueness), appearing on all or part of the membrane, possibly resembling a milky white veil or appearing milky.

[0052] Note: It is necessary to check that blood clotting is not confused with changes in the physicochemical properties of the test substance in aqueous solution (such as the formation of colloids, precipitation, etc.).

[0053] (3) Vascular dissolution: The disappearance of blood vessels on the CAM membrane may be due to multiple factors such as hemorrhage and changes in vascular wall tension.

[0054] 4. Experimental materials and equipment

[0055] SPF chicken embryos: White Laihang fertilized chicken embryos. The supplier should be a designated production enterprise of SPF chicken (eggs) for veterinary drug production and testing, as recognized by the science and technology department. The quality of the chicken embryos should meet the requirements of national standards.

[0056] Teflon rings: 12mm in diameter, 2mm in thickness; egg candling device: LED high-brightness cold light source egg candling device; manual rotor; ophthalmic forceps: pointed forceps and round forceps; micro sampler; digital camera: digital camera with macro lens; cling film; scissors; incubator; constant temperature and humidity chamber; conical flask; physiological saline; 75% alcohol.

[0057] 5. Grouping

[0058] Experimental group: The oil-controlling compositions of Examples 1-3 were diluted with pure water to a concentration of 2%;

[0059] Negative control: 0.9% sodium chloride solution;

[0060] Positive control: 0.1% mol / L sodium hydroxide.

[0061] 6. Test Methods

[0062] (1) Experimental procedure: Place the Teflon ring on the chorioallantoic membrane where there are fewer blood vessels, add 40 μl of each group of samples directly to the rubber ring, observe the CAM reaction, and record the time of occurrence of each toxic effect within 5 min of action. Six chicken embryos must be prepared for each sample.

[0063] (2) Observation of results

[0064] Observe and record bleeding, coagulation, and vascularization manifestations, and score them according to their severity.

[0065] (3) Data Analysis

[0066] Calculate the IS value to assess the irritant properties of the test substance; use the following formula to calculate the stimulus score (IS), and retain the result to two decimal places:

[0067]

[0068] In the formula:

[0069] secH (bleeding time): The average time at which bleeding begins to occur as observed on the CAM membrane, measured in seconds (s).

[0070] secL (vascular dissolution time): The average time at which vascular dissolution begins to occur as observed on the CAM membrane, measured in seconds (s).

[0071] secC (clotting time): The average time for clotting to begin to occur as observed on the CAM membrane, measured in seconds (s).

[0072] Note: If no toxic effect is observed within 5 minutes, the time is calculated as 301 seconds (s).

[0073] Based on the calculated IS values, the eye irritation of the test substances is classified according to the criteria in Table 2 below.

[0074] Table 2 Classification Criteria

[0075] Stimulus rating Irritant Classification IS=0 Non-irritating 0 < IS < 1 Mild irritation 1≤IS<5 Mild irritation 5≤IS<9 moderate irritation 9≤IS<10 Moderate intensity irritant IS≥10 Strongly irritating / corrosive

[0076] 7. Test Results

[0077] The experimental results are shown in Table 3.

[0078] Table 3. Experimental Results

[0079] sample IS value Irritating Example 1 0.00 Non-irritating Example 2 0.00 Non-irritating Example 3 0.00 Non-irritating negative control 0.00 Non-irritating Positive control 10.50 Strong irritant

[0080] As shown in Table 3, the oil-controlling compositions of the present invention are all non-irritating.

[0081] 5α-Reductase Inhibition Rate Test

[0082] 1. Experimental Objective and Principle

[0083] Sebum secretion is closely related to sebaceous gland function, which in turn is closely related to androgen regulation. 5α-reductase is a membrane protease located on microsomes and the cell nucleus. Using the reduced coenzyme NADPH as a hydrogen donor, it catalyzes the conversion of testosterone to dihydrotestosterone (DHT), which induces excessive sebum secretion from the sebaceous glands. Inhibiting 5α-reductase activity reduces DHT production and lowers sebum secretion levels. Through experimental design, if the enzyme activity of 5α-reductase in the test sample is lower than that in the negative control (physiological saline), it indicates that the catalytic rate of 5α-reductase in the negative control (physiological saline) is significantly faster than in the test sample. Therefore, the test sample has a certain inhibitory effect on 5α-reductase, thus achieving an oil-controlling effect.

[0084] 2. Test Indicators

[0085] The enzyme activity of the test sample was compared with that of the negative control (physiological saline). The enzyme activity of the test sample was lower than that of the negative control (physiological saline), indicating that the test sample had a significant inhibitory effect on 5α-reductase.

[0086] 3. Test Methods

[0087] Test samples: The oil-controlling compositions of each embodiment and comparative example were diluted with deionized water to a concentration of 10 wt%.

[0088] Negative control: physiological saline, concentration 10 wt%.

[0089] Sample pretreatment: Take 20 µL of sample solution and negative control (physiological saline) respectively, add them to the enzyme reaction system for catalysis, and measure the absorbance at 340 nm under a UV spectrophotometer. The test is repeated three times, and the average absorbance is used to calculate the enzyme activity. The 5α-reductase inhibition rate is calculated using the following formula.

[0090]

[0091] 4. Test Results

[0092] The experimental results are shown in Table 4;

[0093] Table 4 5α−reductase inhibition rate

[0094] sample Enzyme activity 5α−reductase inhibition rate / % Example 1 4.624 28.18 Example 2 4.876 24.26 Example 3 4.925 23.50 Comparative Example 1 5.218 18.95 Comparative Example 2 5.284 17.92 Comparative Example 3 5.229 18.78 Comparative Example 4 5.325 17.29 Comparative Example 5 5.199 19.25 Comparative Example 6 5.298 17.71 negative control 6.438 /

[0095] As shown in Table 4, the oil-controlling compositions of Examples 1 to 3 of the present invention all have good 5α-reductase inhibition rates, with Example 1 showing a 5α-reductase inhibition rate as high as 28.18%, indicating that it has a very good oil-controlling effect.

[0096] Analysis of the data from Example 1 and Comparative Examples 1-6 shows that when any one of the components—Bletilla striata root hydrosol, kombucha fermentation broth, or oat kernel extract solution—is missing from the composition, the 5α-reductase inhibition rate of the composition decreases significantly, from 28.18% to approximately 18%, indicating that the oil-controlling effect of the composition is poor at this time. Furthermore, data from Comparative Examples 4-6 shows that when Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution are used individually, the kombucha fermentation broth is the most effective. The kombucha fermentation broth content in Comparative Examples 1 and 3 is higher than that in Example 1, and theoretically, its 5α-reductase inhibition rate should also be higher than that in Example 1. However, in reality, its 5α-reductase inhibition rate is much lower than that in Example 1. The above analysis demonstrates that the oil-controlling composition of the present invention, by combining Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution, has a synergistic effect in increasing the 5α-reductase inhibition rate of the composition, thus exerting a synergistic oil-controlling effect. All three components are indispensable.

[0097] Human body testing:

[0098] The oil-controlling composition of Example 1 was diluted with deionized water to a concentration of 10 wt%, and the following tests were performed.

[0099] Six participants with oily skin and acne were selected. On the day of the test, no products were applied to the participants' faces. Before the test, the participants cleansed their faces, dried them with tissues, and sat quietly for 30 minutes in a temperature- and humidity-controlled environment. The tester recorded the type and score of facial lesions before sample application using the Comprehensive Acne Grading System (GAGS), took photos with a VISIA-CR camera, and recorded skin oil levels using a skin oil testing probe. Participants used the sample twice daily, with follow-up tests conducted on days 14 and 28. Measurements for the same participant were performed by the same tester. The rate of change in skin oil levels and the acne removal rate before and after use were used as evaluation indicators.

[0100] The Comprehensive Acne Grading System (GAGS) divides acne-prone areas into five zones: Zone I: Forehead (2 points); Zone II: Right cheek (2 points); Zone III: Left cheek (2 points); Zone IV: Nose (1 point); Zone V: Jawline (1 point). It also grades 5 severity of lesions: 0 points = no lesions; 1 point ≥ 1 comedo; 2 points ≥ 1 papule; 3 points ≥ 1 pustule; 4 points ≥ 1 nodule / cyst. Each zone's score = zone factor score × lesion score (the principle is that the score for the zone is determined by the lesion with the most severe inflammatory response). The sum of the five zone scores is the total score. The severity of lesions is categorized as follows: Figure 1 As shown.

[0101] The test results are shown in Tables 5 and 6. Figures 2-7 As shown;

[0102] Figures 2-7 VISIA-CR photographs of subjects ① to ⑥ at D0, D14, and D28.

[0103] Table 5. Therapeutic index of the oil-controlling composition in Example 1

[0104] Therapeutic effect index D14 D28 Subject ① 87.50% 93.75% Subject ② 59.09% 81.82% Subject ③ 12.50% 50.00% Subject 4 33.33% 66.67% Subject 5 58.33% 83.33% Subject 6 50.00% 75.00% average value 50.13% 75.09%

[0105] Note: Efficacy index = (Total score before treatment - Total score after treatment) / Total score before treatment × 100%;

[0106] Cure: Skin lesions subside, or only pigmentation remains, with a cure rate ≥90%;

[0107] Significant effect: Most skin lesions subside, with a cure rate of 60% ≤ efficacy index < 90%;

[0108] Effective: Partial regression of skin lesions, with efficacy index 20% ≤ efficacy index < 60%;

[0109] Ineffective: Skin lesions do not improve significantly, efficacy index is <20%, or skin lesions worsen.

[0110] Table 6 Acne-removing rate of the oil-controlling composition in Example 1

[0111] time D0 D14 D28 cure rate / 0.00% 16.67% Increased saturation / 16.67% 83.33% efficiency / 83.33% 100% Oil change rate / -7.18% -17.22%

[0112] Note: Acne removal rate:

[0113] Cure rate = (Number of cured cases / Total number of cases) × 100%;

[0114] Cure rate = (Number of cured cases + Number of cases with significant improvement) / Total number of cases × 100%;

[0115] Effective rate = (Number of cured cases + Number of cases with significant improvement + Number of cases with improvement) / Total number of cases × 100%;

[0116] , The average value is the average value of 6 subjects.

[0117] As shown in Tables 5 and 6, the oil-controlling composition of Example 1 of the present invention had a cure rate of 16.67%, an effectiveness rate of 83.33%, and an oil change rate of -7.18% on day 14. On day 28, the cure rate was 16.67%, the cure rate was 83.33%, the effectiveness rate was as high as 100%, and the oil change rate was -17.22%. The above data show that the oil-controlling composition of Example 1 of the present invention has an excellent oil-controlling effect.

[0118] Application example: An oil-control spray

[0119] The formula is shown in Table 7. The preparation method is to mix the oil-control composition, PHC and deionized water, stir evenly at 25°C, and prepare a spray to obtain the oil-control spray.

[0120] Table 7. Oil Control Spray Formulation Table

[0121] Raw material name wt% Deionized water TO100 PHC 0.50 Oil-controlling composition of Example 1 10

[0122] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. An oil-controlling composition, characterized in that, The oil-controlling composition comprises the following components: Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution; the mass ratio of Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution is 1~10:1~5:1~5.

2. The oil-controlling composition according to claim 1, characterized in that, The mass ratio of the Bletilla striata root hydrosol, kombucha fermentation broth, and oat kernel extract solution is 5:3:

2.

3. The oil-controlling composition according to claim 1, characterized in that, The Bletilla striata root hydrosol contains, by weight percentage, 99 wt% Bletilla striata root water, 0.5 wt% p-hydroxyacetophenone and 0.5 wt% 1,2-hexanediol.

4. The oil-controlling composition according to claim 1, characterized in that, The kombucha fermentation broth contains, by weight percentage, 0.05 wt% yeast / Acetobacter xylinum / black tea fermentation product, 40 wt% butylene glycol and 59.95 wt% water.

5. The oil-controlling composition according to claim 1, characterized in that, The oat kernel extract solution contains 0.55 wt% oat kernel extract and 99.45 wt% glycerol by weight percentage.

6. Use of the oil-controlling composition according to any one of claims 1 to 5 in the preparation of cosmetics.

7. The use according to claim 6, characterized in that, The cosmetic product in question is for oil control.

8. A cosmetic product, characterized in that, The cosmetic contains the oil-controlling composition as described in any one of claims 1 to 5.

9. The cosmetic product according to claim 8, characterized in that, The content of the oil-control composition in the cosmetic is 5-15 wt%.

10. The cosmetic product according to claim 8, characterized in that, The cosmetic product is in one of the following forms: emulsion, liquid, cream, or spray.

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