Cu-Ag / SiO2-coated PDMS composite antibacterial osseointegration promoting coating as well as preparation method and application thereof

Abstract

The invention discloses a Cu-Ag / SiO2-coated PDMS composite antibacterial osseointegration promoting coating and a preparation method and application thereof, and belongs to the technical field of biomedical materials and surface engineering.The preparation method comprises the steps that S1, copper salt and silver salt are dissolved in a solvent to prepare a mixed metal precursor solution; s2, mixing the mixed metal precursor solution with PDMS (Polydimethylsiloxane) organic silicon resin containing SiO2 nanoparticles to form a precursor composite system; s3, a reducing agent is added into the precursor composite system, metal ions are subjected to in-situ reduction in the PDMS-SiO2 hybrid network and are subjected to domain limitation, and Cu-Ag bimetallic nanoparticles are formed; and S4, applying the system to the surface of a pretreated matrix, and curing to form the composite antibacterial osseointegration promoting coating. The method is mild in process condition, simple in step, easy to regulate and control, suitable for spraying, spin coating, dip coating and other coating modes, and capable of being applied to surface functional modification of orthopedic and oral titanium-based implants.

Description

Technical Field

[0001] This invention belongs to the field of biomedical materials and surface engineering technology, specifically relating to a Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integrative coating, its preparation method, and its application. Background Technology

[0002] Titanium and titanium alloys are widely used as the preferred materials for orthopedic and dental implants due to their excellent mechanical properties, good biocompatibility, and stable corrosion resistance. However, as bioinert materials, titanium and titanium alloys do not possess inherent antibacterial capabilities. Once bacteria colonize and biofilms form on the implant surface, implant-related infections are highly likely to occur, which is one of the leading causes of postoperative complications, implant failure, and even revision surgery. This not only increases patient suffering and financial burden but also poses a serious challenge to clinical treatment.

[0003] Currently, common strategies for antibacterial modification of implant surfaces mainly include the following categories: First, loading antibiotics through physical adsorption or chemical bonding, but this has problems such as rapid burst release, induction of bacterial resistance, and potential allergic reactions; second, constructing antibacterial coatings based on single metal ions such as silver, zinc, and copper, however, such coatings often have limitations such as uncontrollable metal ion release, potential cytotoxicity, insufficient long-term effectiveness, and potential interference with the normal bone healing process; third, introducing organic antibacterial components such as quaternary ammonium salts and chitosan, which generally have disadvantages such as poor chemical stability, short duration of action, and insufficient bonding strength with the matrix. Overall, most existing technologies struggle to achieve a good balance between the two key properties of "long-lasting antibacterial effect" and "biosafety / osteoporosis promotion."

[0004] Therefore, developing novel functional coatings for implant surfaces that exhibit synergistic mechanisms and combine long-lasting antibacterial properties with excellent osteointegration capabilities has become an important and urgent research direction in the field of biomaterials. This can not only effectively reduce the risk of infection and improve the success rate of implantation surgery, but also has significant implications for promoting the clinical application of functional implantable devices. Summary of the Invention

[0005] The present invention aims to provide a Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integration coating, its preparation method, and its application. This coating achieves nano-confinement and interface regulation by constructing a PDMS-SiO2 hybrid network, enabling uniform distribution and stable immobilization of Cu-Ag bimetallic nanoparticles within the PDMS-SiO2 hybrid matrix, thus achieving long-term, controllable release of metal ions. This design aims to synergistically leverage the broad-spectrum antibacterial efficacy and osteogenic activity of Cu and Ag, effectively promoting bone tissue integration while inhibiting bacterial infection on the implant surface, thereby improving the clinical success rate and long-term stability of the implant.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: This invention provides a method for preparing a Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating, comprising the following steps: S1: Dissolve copper salt and silver salt in a solvent in a certain proportion to prepare a mixed metal precursor solution; S2: The mixed metal precursor solution is added to polydimethylsiloxane (PDMS) silicone resin containing SiO2 nanoparticles, and then ultrasonically dispersed and stirred to form a uniformly dispersed precursor composite system. S3: Under inert atmosphere and low temperature conditions, a reducing agent is added to the precursor composite system to reduce and confine copper and silver ions in situ in the PDMS-SiO2 hybrid network, forming Cu-Ag bimetallic nanoparticles. S4: The obtained system is applied to the surface of a pretreated titanium or titanium alloy substrate by spraying, spin coating or dip coating, and then thermosetting to form a composite antibacterial and osteointegrative coating that is stably bonded to the substrate.

[0007] In step S1, the copper and silver salts include copper-based and silver-based salts that can be dissolved in aqueous solutions or alcohol solvents, specifically including copper nitrate (anhydrous / hydrated), copper sulfate (anhydrous / hydrated), silver nitrate, silver acetate, etc., and the purity of the salts is not lower than that of analytical grade, so as to avoid the interference of impurities on the antibacterial properties and biocompatibility of the coating.

[0008] In step S1, copper salt and silver salt are dissolved in a solvent at a molar ratio (atomic ratio) of Cu to Ag of 5:1 to 1:1, preferably 3:1 to 1:1, to prepare a mixed metal precursor solution.

[0009] In step S1, the solvent includes water, anhydrous ethanol, ethylene glycol, and glycerol, and polyvinylpyrrolidone (PVP) is added to the system as a dispersant and film-forming aid, with a mass fraction of 0.5% to 5% of the total solvent mass, to improve the dispersion stability of metal salt particles and enhance the adhesion between the coating and the titanium alloy substrate.

[0010] In step S2, the particle size of SiO2 nanoparticles is 10nm to 200nm.

[0011] In step S3, the low temperature condition refers to the condition of 0℃~5℃.

[0012] In step S3, the reducing agent includes ascorbic acid, sodium borohydride, hydrazine hydrate, polyol reducing agent, sugar reducing agent, and natural plant extract reducing agent; the polyol reducing agent includes ethylene glycol and glycerol, and the natural plant extract reducing agent includes tea polyphenols and gallic acid.

[0013] In step S4, the substrate is a titanium or titanium alloy substrate, and the pretreatment includes one or more combinations of grinding, pickling, and passivation.

[0014] In step S4, the curing conditions are: curing temperature 50℃~200℃, curing time 1h~6h, and curing atmosphere is air, nitrogen or inert gas.

[0015] The composite antibacterial and osteogenic integrative coating (hereinafter referred to as the composite coating) obtained by the above preparation method comprises a continuously distributed PDMS organosilicon matrix, SiO2 nanoparticles uniformly dispersed in the matrix, and Cu-Ag bimetallic nanoparticles generated in situ and confined in the PDMS-SiO2 network structure. The composite coating has a continuous and dense structure with a thickness of 1 μm to 50 μm.

[0016] The PDMS-SiO2 hybrid network provides structural confinement and stable loading for the Cu-Ag bimetallic nanoparticles, ensuring their uniform distribution and slow release within the composite coating. The Cu-Ag bimetallic nanoparticles achieve broad-spectrum antibacterial function through the synergistic effect of Cu and Ag. While maintaining antibacterial efficacy, the controlled silver content effectively reduces adverse effects on surrounding tissue cells. Therefore, this composite coating possesses excellent antibacterial properties, biocompatibility, and tissue compatibility, making it suitable as a surface functional modification layer for orthopedic and dental titanium or titanium alloy implants, and applicable for long-term in vivo application of titanium and titanium alloy implants.

[0017] This composite coating, through the sustained-release effect of bimetallic nanoparticles in a hybrid network, endows the implant with highly efficient broad-spectrum antibacterial properties with an inhibition rate of over 99.5% against common pathogenic bacteria such as Staphylococcus aureus and Escherichia coli, achieving sustained inhibition of pathogenic bacteria. At the same time, its osteointegration function stems from the synergistic effect of SiO2 nanoparticles and metal ions, which can effectively regulate the behavior of osteoblast-related cells, significantly promote the adhesion, spread and proliferation of osteoblasts, and thus enhance the integration efficiency between the implant and the surrounding bone tissue.

[0018] Based on this, the present invention also provides the application of the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integration coating in the preparation of a functional modification layer on the surface of orthopedic or dental titanium and titanium alloy implants. The implants are suitable for long-term in-vivo implantation and can improve the safety and long-term stability of the implants.

[0019] Compared with the prior art, the present invention has the following beneficial effects: (1) Synergistic enhancement of antibacterial properties: Through the bimetallic synergistic antibacterial mechanism formed by Cu and Ag, the composite coating achieves a high-efficiency and broad-spectrum antibacterial effect against a variety of common pathogens while significantly reducing the amount of silver used, and effectively reduces the risk of cytotoxicity that may be caused by traditional high-silver-content coatings.

[0020] (2) Stable and controllable structure and release: Cu-Ag nanoparticles are confined and loaded in situ using PDMS-SiO2 hybrid network, which not only avoids the aggregation and rapid release of nanoparticles, but also realizes the long-term, stable and controllable release of active ions, thereby ensuring the long-lasting antibacterial function of the coating.

[0021] (3) Excellent biocompatibility and osteogenic activity: The surface characteristics and component design of the coating have good cell compatibility, which can significantly promote the adhesion, spread, proliferation and osteogenic gene expression of osteoblasts on its surface, thereby effectively improving the integration efficiency between the implant and bone tissue.

[0022] (4) Excellent process adaptability and application potential: The preparation method has mild process conditions, simple steps and easy control. It is suitable for various coating methods such as spraying, spin coating and dip coating. It has good process adaptability and large-scale production potential. It can be widely used in the surface functionalization modification of orthopedic and oral titanium-based implants. Attached Figure Description

[0023] Figure 1 This is a schematic diagram illustrating the synthesis and coating process of the Cu-Ag / SiO2@PDMS composite coating prepared in Example 1 of the present invention. Figure 2 The images show the morphology and elemental distribution of Cu-Ag bimetallic nanoparticles and SiO2 nanoparticles in the composite coating prepared in Example 1 of this invention; where A: low-magnification overall morphology of the composite coating (simultaneously showing Cu-Ag bimetallic nanoparticles and SiO2 nanoparticles); B: low-magnification scanning electron microscope morphology of SiO2 nanoparticles; C: high-magnification scanning electron microscope morphology of SiO2 nanoparticles; F: scanning electron microscope morphology of Cu-Ag bimetallic nanoparticles; D, E, G, H, I: elemental distribution of Cu-Ag bimetallic nanoparticles; Figure 3 The graph shows the electrochemical impedance spectroscopy results of the Cu-Ag / SiO2@PDMS composite coating in this invention; where A: Nyquist plot; B: Bode plot; Figure 4 The figure shows the in vitro antibacterial performance test results of the Cu-Ag / SiO2@PDMS composite coating in this invention; where A: antibacterial effect against Staphylococcus aureus; B: antibacterial effect against Escherichia coli; C: inhibition rate against Staphylococcus aureus; D: inhibition rate against Escherichia coli. Figure 5The figures show the in vitro observation results of osteoblast adhesion and survival on different implant surfaces in this invention; where A: osteoblast status on the metal surface without composite coating; B: osteoblast status on the Cu-Ag / SiO2@PDMS composite coating surface. Figure 6 The diagram shows the in vivo antibacterial and inflammatory response evaluation results of the composite coating in the implant-related infection model of this invention; where A: schematic diagram of the experimental process of the animal bone defect model; B: bacterial culture results of tissues surrounding the implant; C: scanning electron microscope image of bacterial adhesion on the implant surface; D: CD68 immunohistochemical staining results of tissues surrounding the implant; E: iNOS immunohistochemical staining results of tissues surrounding the implant. Figure 7 The images show the imaging and histological analysis results of the Cu-Ag / SiO2@PDMS composite coating promoting osseointegration in vivo in this invention; where A: histological section results of major organs (heart, liver, spleen, lung, kidney); B: imaging reconstruction results of bone tissue around the implant; C: histological section results of the implant-bone interface. Detailed Implementation

[0024] The present invention will be further illustrated by the following embodiments. It should be understood that the embodiments are merely illustrative of the specific implementation and effects of the present invention and are not intended to limit the scope of protection of the present invention.

[0025] Unless otherwise specified, the experimental methods used in the embodiments were performed in accordance with conventional techniques in the art; the reagents and materials involved were all commercially available conventional products.

[0026] Each embodiment included three or more parallel experiments, with data expressed as mean ± standard deviation to ensure the reliability and accuracy of the experimental results.

[0027] Example 1 Preparation of Cu-Ag / SiO2@PDMS composite coating: This embodiment provides a method for preparing a Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating, the steps of which are as follows: (1) Preparation of mixed metal precursor solution: Weigh out 0.16 g of copper nitrate trihydrate and 0.042 g of silver nitrate to make the molar ratio of Cu to Ag about 2.7:1. Dissolve them together in 20 mL of ethanol / deionized water mixed solvent (volume ratio 1:1), and add 1 wt% polyvinylpyrrolidone. Stir magnetically for 30 minutes at room temperature to obtain a homogeneous and stable mixed metal precursor solution.

[0028] (2) Construction of precursor complex system: Take 100 mg of SiO2 nanoparticles with an average particle size of approximately 50 nm and add them to a mixture of 10 g PDMS resin and Sylgard 184 curing agent (resin to curing agent mass ratio of 10:1). After thorough mixing, add all of the mixed metal precursor solution obtained in step (1). Then, ultrasonically disperse the resulting system for 30 minutes and continue magnetic stirring for 2 hours to obtain a uniform precursor composite system.

[0029] (3) In-situ reduction and confined formation of Cu-Ag bimetallic nanoparticles: The aforementioned precursor composite system was transferred to a three-necked flask, and nitrogen gas was introduced to create an inert protective environment. The temperature was then lowered to 0℃–5℃ under ice-water bath conditions. Under continuous stirring, 10 mL of a 0.1 mol / L ascorbic acid aqueous solution was slowly added dropwise. After the addition was complete, the reaction continued under nitrogen protection for 2 hours. The system color was observed to gradually change from light to a stable brownish-red, indicating that Cu-Ag bimetallic nanoparticles had been generated in situ within the PDMS-SiO2 hybrid network and were effectively confined.

[0030] (4) Coating and curing: The Ti6Al4V substrate, which had been ultrasonically cleaned with acetone and ethanol and dried with nitrogen, was fixed in a spraying device. The composite system obtained in step (3) was uniformly sprayed onto the surface of the substrate at a spraying pressure of 0.3 MPa and a spraying distance of 15 cm. After spraying, the substrate was heat-cured at 120°C for 2 hours, and after cooling, the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating was obtained.

[0031] The composite coating structure obtained by the above method is shown in the figure. Figure 1 As shown, the morphology and elemental distribution of the nanoparticles in the coating are as follows: Figure 2 As shown.

[0032] Example 2 Corrosion resistance and electrochemical stability evaluation (EIS): To evaluate the long-term stability and corrosion resistance of the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating in a simulated body fluid environment, this embodiment uses electrochemical impedance spectroscopy (EIS) to test the composite coating samples prepared in Example 1, and uses uncoated samples or samples coated only with PDMS-SiO2 coating as controls.

[0033] In the experiment, the sample was immersed in simulated body fluid (SBF) at 37°C for 30 days to simulate the long-term service state of the implant in the in vivo environment. After immersion, electrochemical tests were performed using a three-electrode system, with the sample as the working electrode, a platinum sheet as the counter electrode, and a saturated calomel electrode as the reference electrode. Electrochemical impedance spectroscopy was performed under open-circuit potential conditions, with an AC perturbation voltage amplitude of 10 mV and a test frequency range of 10 Hz. 5 Hz to 10 -2 Hz.

[0034] The Nyquist and Bode plots obtained from the test are as follows: Figure 3 As shown. By Figure 3 As can be seen, compared with the control sample, the sample coated with Cu-Ag / SiO2@PDMS composite coating exhibits a larger impedance modulus and a more stable phase angle characteristic throughout the entire test frequency range, indicating that the composite coating still maintains good electrochemical stability under long-term SBF immersion conditions.

[0035] The above results demonstrate that the confinement of Cu-Ag bimetallic nanoparticles by the PDMS-SiO2 hybrid network can impart antibacterial function to the coating while maintaining its corrosion resistance and structural integrity in physiological environments, thus meeting the requirements for long-term use of implants in vivo.

[0036] Example 3 In vitro antibacterial performance evaluation of Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating: To verify the inhibitory effect of the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating prepared in Example 1 on common pathogenic bacteria, Staphylococcus aureus and Escherichia coli were selected as representative Gram-positive and Gram-negative bacteria, respectively. The in vitro antibacterial performance of the composite coating was tested according to ISO 22196:2011 "Measurement of antibacterial activity on plastics and other non-porous surfaces".

[0037] In the experiment, the composite coating samples were placed in sterile culture plates, and pre-prepared solutions with a concentration of 10 were added. 6 A bacterial suspension of CFU / mL was used to ensure full contact between the sample surface and the bacterial solution, and the samples were incubated at 37°C for 2–6 hours. Samples without coating or without a PDMS-SiO2 coating containing Cu-Ag bimetallic nanoparticles served as control groups. After incubation, the bacteria on the sample surface were eluted, and the number of surviving colonies on the surface of each group of samples was counted using a plate count method.

[0038] Experimental results showed that under different culture time conditions, the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating significantly reduced the number of Staphylococcus aureus and Escherichia coli on the sample surface, with an inhibition rate of over 99.5%. In contrast, the control group samples did not show a significant antibacterial effect under the same conditions. The statistical results of bacterial colony morphology and inhibition rate are arranged in the order of experiment and photograph, as follows: Figure 4 As shown.

[0039] The above results demonstrate that the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating of the present invention exhibits stable and long-lasting broad-spectrum antibacterial properties against both Gram-positive and Gram-negative bacteria under in vitro conditions.

[0040] Example 4 In vitro osteogenic compatibility evaluation of Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integration coating: To evaluate the effect of the Cu-Ag / SiO2@PDMS composite antibacterial osteopromoting coating on osteogenic-related cell behavior, this embodiment observes the adhesion, growth, and survival status of osteoblasts on different sample surfaces in vitro.

[0041] In the experiment, MC3T3-E1 osteoblasts were seeded onto the surface of the Cu-Ag / SiO2@PDMS composite antibacterial osteogenic integrative coating sample prepared in Example 1, with uncoated samples or samples coated only with PDMS-SiO2 coating serving as controls. After culturing for 72 hours, the cells were fixed, and the distribution of cells on the sample surface was analyzed by microscopic observation and fluorescence staining.

[0042] Experimental results showed that cells adhered well to the composite coating surface and spread out, exhibiting intact cell morphology, and the cell number gradually increased with increasing culture time. Fluorescence staining results showed that the composite coating surface was dominated by viable cell signals, with no obvious cell damage or death observed. The relevant cell morphology and viability results are arranged in the order of photographing, as follows: Figure 5 As shown.

[0043] The above results indicate that the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integrative coating maintains its antibacterial function while also exhibiting good in vitro biocompatibility, providing a suitable adhesion and growth interface for osteoblast-related cells.

[0044] Example 5 In vivo verification of the antibacterial and osteointegrative properties of Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating: The antibacterial properties, biosafety, and osteointegration ability of the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegration-promoting coating prepared in Example 1 were comprehensively evaluated in vivo using an animal bone defect model.

[0045] In the experiment, implants coated with the composite coating were inserted into bone defects in experimental animals, with uncoated implants or implants coated only with PDMS-SiO2 serving as controls. Samples were taken 3 days post-operation for observation of bacterial adhesion and immunohistochemical staining analysis of inflammation-related markers in the tissue surrounding the implants.

[0046] The results showed that, compared with the control group, the number of bacteria adhering to the implant was significantly reduced and the expression levels of inflammation-related markers were lower in the peri-implant tissue coated with Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating. The relevant in vivo antibacterial effects and inflammatory response assessment results are listed in the order of sampling and photography, such as... Figure 6 As shown.

[0047] Furthermore, the formation of new bone tissue around the implant was analyzed using imaging and histological methods. The results showed that continuous new bone tissue appeared around the implant in the composite coating group, forming a good bond at the bone-implant interface, while the control group showed less new bone formation. Relevant imaging reconstruction results and histological section results are arranged in the order of sampling and photographing, as follows: Figure 7 As shown.

[0048] The above results indicate that the Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating of the present invention can effectively inhibit implant-related infections in the in vivo environment while promoting the formation of new bone tissue and bone-implant interface integration, demonstrating good biosafety and application prospects.

Claims

1. A method for preparing a Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating, characterized in that, Includes the following steps: S1: Dissolve copper salt and silver salt in a solvent to prepare a mixed metal precursor solution; S2: The mixed metal precursor solution is added to PDMS silicone resin containing SiO2 nanoparticles, and then ultrasonically dispersed and stirred to form a uniformly dispersed precursor composite system. S3: Under inert atmosphere and low temperature conditions, a reducing agent is added to the precursor composite system to reduce and confine copper and silver ions in situ in the PDMS-SiO2 hybrid network, forming Cu-Ag bimetallic nanoparticles. S4: The obtained system is applied to the pretreated substrate surface by spraying, spin coating or dip coating, and then thermosetting to form a composite antibacterial and osteointegrative coating that is stably bonded to the substrate.

2. The preparation method of the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 1, characterized in that, In step S1, the copper salt and silver salt include anhydrous and hydrated copper nitrate, anhydrous and hydrated copper sulfate, silver nitrate, and silver acetate.

3. The method for preparing the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 1, characterized in that, In step S1, copper salt and silver salt are dissolved in a solvent at a molar ratio of Cu to Ag of 5:1 to 1:1 to obtain a mixed metal precursor solution. The solvent includes water, anhydrous ethanol, ethylene glycol, and glycerol, and polyvinylpyrrolidone is added to the system at a mass fraction of 0.5% to 5% of the total mass of the solvent.

4. The method for preparing the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 1, characterized in that, In step S2, the particle size of SiO2 nanoparticles is 10nm to 200nm.

5. The method for preparing the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 1, characterized in that, In step S3, the low temperature condition refers to the condition of 0℃~5℃; the reducing agent includes ascorbic acid, sodium borohydride, hydrazine hydrate, polyol reducing agent, sugar reducing agent, and natural plant extract reducing agent; wherein, the polyol reducing agent includes ethylene glycol and glycerol, and the natural plant extract reducing agent includes tea polyphenols and gallic acid.

6. The preparation method of the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 1, characterized in that, In step S4, the substrate is a titanium or titanium alloy substrate, and the pretreatment is one or more combinations of grinding, pickling, and passivation.

7. A Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating, characterized in that, The Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic integrative coating is prepared by any one of claims 1-6. The composite antibacterial and osteogenic integrative coating comprises a continuously distributed PDMS organosilicon matrix, SiO2 nanoparticles uniformly dispersed in the matrix, and Cu-Ag bimetallic nanoparticles generated in situ and confined in the PDMS-SiO2 network structure. The composite antibacterial and osteogenic integrative coating has a continuous and dense structure with a coating thickness of 1 μm to 50 μm.

8. The Cu-Ag / SiO2@PDMS composite antibacterial and osteointegrative coating according to claim 7, characterized in that, This composite antibacterial and osteointegrative coating has an inhibition rate of over 99.5% against Staphylococcus aureus and Escherichia coli, and also possesses broad-spectrum antibacterial properties, biocompatibility, and tissue compatibility.

9. The application of the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating as described in claim 7 or 8 in the preparation of a functional modification layer for the surface of orthopedic or dental titanium and titanium alloy implants.

10. The application of the Cu-Ag / SiO2@PDMS composite antibacterial and osteogenic coating according to claim 9 in the preparation of a functional modification layer for the surface of orthopedic or dental titanium and titanium alloy implants, characterized in that, The implant is suitable for long-term implantation within the body.

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