Bean crop seedling stage salt tolerance identification device convenient to observe

Abstract

A bean crop seedling stage salt tolerance identification device convenient to observe relates to the technical field of breeding tests and is used for solving the problems that bean seedlings cannot stand and the whole-stage observation of the growth process cannot be carried out when salt tolerance identification is carried out on the bean crop seedling stage by utilizing an existing culture tray. Comprising seed strips, lifting baskets and culture boxes, the seed strips are telescopic transparent pieces, planting cavities are formed in the seed strips, and seed supports with holes are arranged in the planting cavities; the lifting basket is provided with a placing groove, the bottom of the lifting basket is provided with a bottom hole communicated with the placing groove, after seeds are placed in the planting cavity of the seed branches, the seed branches are placed in the placing groove, and then the lifting basket is placed in a culture box filled with a nutrient solution or saline water. The telescopic seed strips are arranged, so that the growth supporting requirements of seeds in the seedling emergence stage and the seedling stage are met, and support is provided for young stems. And the accuracy of salt tolerance identification is improved by carrying out uniform stress in cooperation with the basket.

Description

Technical Field

[0001] This invention relates to the field of breeding experiment technology, specifically to a device for identifying salt tolerance in legume seedlings that is easy to observe. Background Technology

[0002] Seed salt tolerance testing is an important research method for assessing the germination and early growth ability of plant seeds under salt stress, and is often used to screen salt-tolerant varieties or elucidate molecular mechanisms. Because legume seedlings are slender and weak with poor support, they are more prone to lodging and curling under salt stress, leading to problems such as roots being unable to contact the stress solution, cotyledon rotting, and damage to the growing point, severely affecting the accuracy of identification results. Existing circular culture trays cannot be used for salt tolerance identification of legume seeds because they cannot provide support. Furthermore, existing culture trays are opaque and have limited space, making it difficult to achieve in-situ observation and data collection of root development dynamics, and failing to meet the needs of modern molecular breeding for precise phenotypic identification. Therefore, there is an urgent need to develop an identification device specifically designed for salt tolerance identification of legume seedlings, providing both support and observation functions. Summary of the Invention

[0003] The purpose of this invention is to provide a convenient device for assessing salt tolerance in legume seedlings. This addresses the problem encountered when using existing culture trays to assess salt tolerance in legume seedlings, where the young stems are delicate and weak, easily lodging, preventing contact with the stress solution, causing cotyledon rot, and damaging the growing point. The device of this invention meets the support requirements of legume seeds from germination to seedling growth, while ensuring that the germination, root development, and stem and leaf growth of each seed can be clearly observed and recorded.

[0004] The technical solution adopted by the present invention to solve its technical problem is: a salt tolerance identification device for legume seedlings that is easy to observe, including a seed strip, a basket, and a culture box; the seed strip is a retractable transparent part, the seed strip has a planting cavity, and the planting cavity has a perforated seed holder; the basket has a placement groove, and the bottom of the basket has a bottom hole communicating with the placement groove. After placing seeds in the planting cavity of the seed strip, the seed strip is placed in the placement groove, and then the basket is placed in a culture box containing nutrient solution or saline solution.

[0005] Furthermore, the seed strip is generally rectangular, with its upper and lower ends having a rigid structure and its middle section having a corrugated portion.

[0006] Furthermore, the seed strip has through holes at both the upper and lower ends, and a telescopic cylinder is provided inside the corrugated part of the seed strip. The top of the telescopic cylinder is in contact with and fixed to the upper end of the seed strip, and the bottom of the telescopic cylinder is in contact with and fixed to the lower end of the seed strip. The through holes and the inner cavity of the telescopic cylinder constitute a planting cavity.

[0007] Furthermore, the seed strip has a length-fixing mechanism at both ends. The length-fixing mechanism includes a screw and a nut. The nut of the screw is fixedly connected to the lower end of the seed strip. The screw passes through the upper end of the seed strip and extends out of the seed strip. The nut is set on the screw extending out of the seed strip and contacts the upper end of the seed strip.

[0008] Furthermore, the seed strip has a length-fixing mechanism at both ends, the length-fixing mechanism including an I-shaped shaping card, and the seed strip is located inside the shaping card.

[0009] Furthermore, the middle part of the seed strip has a length-fixing mechanism, the length-fixing mechanism including an I-shaped shaping card, and the seed strip is located inside the shaping card.

[0010] Furthermore, the upper and lower surfaces of the seed strip each have grooves, and the shaping clip extends into the grooves.

[0011] Furthermore, the seed strip includes an upper box and a lower box. The bottom of the upper box is open, and the top of the lower box is open. The upper box contains an upper cylinder, and the lower box contains a lower cylinder. The length and width dimensions of the upper box are the same as the cross-sectional dimensions of the inner cavity of the lower box. The inner cavities of the upper and lower cylinders form a planting cavity. After the upper box is inserted into the lower box, the upper cylinder extends into the lower cylinder, and the seed holder is located inside the upper cylinder.

[0012] Furthermore, the upper surface of the basket has a partition plate that divides the space above the basket into two areas. In each area, there are several clamping partition plates that are equally spaced, and a placement groove is formed between each pair of adjacent clamping partition plates.

[0013] Furthermore, the seed holder is a cross-shaped, star-shaped, or mesh structure.

[0014] The beneficial effects of this invention are as follows: By placing seeds within the planting chamber of the seed strip, this invention provides support for the young stems, preventing them from collapsing due to their thinness, weakness, and poor support. The expandable design of the seed strip can adapt to the support needs of seedlings of different heights, thus meeting the growth needs of seeds at different stages of emergence and seedling development. The transparent structure of the seed strip allows for convenient observation of individual seed emergence and seedling growth by placing different seeds in different planting chambers. This enables 360-degree in-situ, non-destructive observation of root development and stem and leaf growth for each seed, meeting the needs of molecular breeding for precise phenotypic data collection. The basket design allows for uniform assembly of the seed strips. Placing the basket in the culture box provides uniform stress to the seeds within different seed strips, ensuring consistent treatment conditions for each individual plant and significantly improving the accuracy, repeatability, and efficiency of salt tolerance identification. Attached Figure Description

[0015] Figure 1 This is a three-dimensional diagram of a type of embodiment of the present invention; Figure 2 This is a front view of a strip according to Embodiment 1 of the present invention; Figure 3 This is a three-dimensional diagram of a seed strip in Embodiment 2 of the present invention; Figure 4 This is a top view of one embodiment of the present invention; Figure 5 This is a front view of the compressed state of the strip in Embodiment 1 of the present invention; Figure 6 This is a front view of the unfolded state of the strip in Embodiment 1 of the present invention; Figure 7 This is a three-dimensional view of the basket of the present invention; Figure 8 This is a top view of the basket of the present invention; Figure 9 This is a three-dimensional assembly drawing of the seed strip and basket of the present invention; Figure 10 This is a three-dimensional diagram of the identification device of the present invention; Figure 11 This is a front view of a strip according to Embodiment 3 of the present invention; Figure 12 This is a front view of a strip according to Embodiment 4 of the present invention; Figure 13 This is a front view of the prototyping card of the present invention; Figure 14 This is a three-dimensional diagram of a species in Embodiment 5 of the present invention; Figure 15 This is a three-dimensional diagram of a species in Embodiment Six of the present invention; Figure 16 This is a cross-sectional view of Embodiment Six of the present invention; Figure 17 This is a three-dimensional diagram of the unfolded state of the strips in Embodiment Six of the present invention; In the diagram: 1. Seed strip, 10. Groove, 11. Through hole, 12. Corrugated part, 13. Seed holder, 14. Screw, 15. Nut, 16. Telescopic cylinder, 17. Shaping clip, 171. Clearance hole, 18. Upper box, 181. Upper cylinder, 19. Lower box, 191. Lower cylinder, 2. Basket, 21. Handle, 22. Partition plate, 23. Clamping partition, 24. Bottom hole, 25. Placement groove, 3. Culture box. Detailed Implementation

[0016] like Figure 10 As shown, the salt tolerance identification device for legume seedlings of the present invention, which is easy to observe, includes seed strips 1, a basket 2, and a culture box 3. The structure and usage of the present invention will be described in detail below with reference to the accompanying drawings.

[0017] like Figures 1 to 2As shown, this is an embodiment of seed strip 1. Seed strip 1 is generally rectangular in shape and is a transparent plastic part. The upper and lower ends of seed strip 1 are rigid structures, which can be made of transparent plastic sheets. The middle of seed strip 1 has a corrugated part 12, which allows seed strip 1 to expand and contract. Figure 5 As shown, by squeezing the seed strip 1 at both ends, the seed strip 1 can be compressed, at which point the thickness of the seed strip 1 is minimized. Figure 6 As shown, stretching seed strip 1 to both sides allows it to unfold, increasing its thickness. Figure 1 , Figure 2 As shown, a row of equally spaced through holes 11 is provided at both the upper and lower ends of the seed strip 1, and the through holes 11 at the upper and lower ends of the seed strip 1 are aligned vertically. A telescopic cylinder 16 is located between the upper and lower ends of the seed strip 1. The telescopic cylinder 16 is essentially a corrugated pipe. The top of the telescopic cylinder 16 is in contact and fixed to the upper end of the seed strip 11, and the bottom of the telescopic cylinder 16 is in contact and fixed to the lower end of the seed strip 1. Both ends of the telescopic cylinder 16 are connected to the through holes 11. The size of the through holes 11 and the inner diameter of the telescopic cylinder 16 are designed to allow clear observation of the seeds from above. When the seed strip 1 extends or retracts, the telescopic cylinder 16 also extends or retracts accordingly. Figure 4 As shown, a seed holder 13 is provided inside the telescopic cylinder 16. The seed holder 13 has a perforated structure and can be cross-shaped, star-shaped, or mesh-like. The seed holder 13 is designed to support bean seeds. In use, bean seeds are inserted into the through hole 11 at the upper end of the seed strip 1, at which point the bean seeds fall onto the seed holder 13. The through hole 11 and the inner cavity of the telescopic cylinder 16 constitute the planting cavity for placing the seeds. Figure 6 As shown, after stretching the seed strip 1, the planting cavity above the seed holder 13 is the seedling growth zone D1, and the planting cavity below the seed holder 13 is the root growth zone D2.

[0018] In use, place the seeds in the planting chambers, with only one seed in each chamber for easy individual observation. The transparent seed strip 1 allows for side observation of seed germination and seedling morphology. The planting chambers provide support for the seedling stems, preventing them from falling over. The retractable design of the seed strip 1 can meet the support needs of seedlings at different growth stages and heights.

[0019] like Figure 4 As shown, this is a second embodiment of seed strip 1. The difference between embodiment 2 and embodiment 1 is that the through holes 11 in embodiment 2 have two rows. The design of two rows of through holes 11 can hold more seeds. In this case, for easier observation, the two rows of through holes 11 can be staggered.

[0020] like Figure 11As shown, this is Embodiment 3 of the seed strip 1. The difference between Embodiment 3 and Embodiment 1 is that a length-fixing mechanism is provided at both ends of the seed strip 1. The length-fixing mechanism includes a screw 14 and a nut 15. The nut of the screw 14 is fixed inside the lower end of the seed strip 1. The screw 14 passes through the upper end of the seed strip 1 and extends out a section. The nut 15 is fitted onto the screw 14. Due to the elasticity of the corrugated portion 12, the upper end of the seed strip 1 contacts the nut 12. When the nut 15 is screwed onto the screw 14, the position of the nut 15 on the screw 14 can be adjusted, thereby adjusting the height of the seed strip 1 and fixing its length. To ensure that the height of the seed strip 1 is consistent throughout its length, a length-fixing mechanism is provided at each end of the corrugated portion 12. The screw 14 and nut 15 are made of plastic.

[0021] like Figure 12 As shown, this is Embodiment Four of Seed Strip 1. The difference between Embodiment Four and Embodiment One is that a length-fixing mechanism is provided at both ends of Seed Strip 1, as shown in the figure. Figure 13 As shown, the length-fixing mechanism includes a shaping clip 17, which has an "]" shape. The upper end of the shaping clip 17 contacts the upper end of the seed strip 1, and the lower end of the shaping clip 17 contacts the lower end of the seed strip 1. The left and right ends of the seed strip 1 are placed inside the shaping clip 17. The height of the shaping clip 17 determines the height of the seed strip 1, and various sizes of shaping clips 17 can be provided as needed. The shaping clip 17 is made of plastic. To ensure that the shaping clip 17 is coplanar with the upper and lower ends of the seed strip 1, grooves are provided in the upper and lower surfaces of the seed strip 1, and the upper and lower ends of the shaping clip 17 are placed in the corresponding grooves.

[0022] like Figure 14 The image shows a fifth embodiment of seed strip 1. The difference between this fifth embodiment and the first embodiment is that a length-fixing mechanism is provided in the middle of seed strip 1. This mechanism includes a shaping clip 17, which is a "]" shaped structure. The upper end of the shaping clip 17 contacts the upper end of seed strip 1, and the lower end of the shaping clip 17 contacts the lower end of seed strip 1. Both ends of the shaping clip 17 have clearance holes 171 to avoid through holes 11. Grooves 10 are provided at the middle of the upper end and the middle of the lower end of seed strip 1. The two ends of the shaping clip 17 are placed within the corresponding grooves 10, at which point the clearance holes 171 are aligned with the through holes 11.

[0023] like Figure 15 , Figure 16 As shown, this is Embodiment Six of Seed Strip 1. The difference between Embodiment Six and Embodiment One is that the structure of Seed Strip 1 is different. In Embodiment Six, Seed Strip 1 includes an upper box 18 and a lower box 19, as follows: Figure 16As shown, the upper box 18 is a rectangular hollow plastic part with an open bottom, and the lower box 19 is a rectangular hollow plastic part with an open top. Both the upper box 18 and the lower box 19 are transparent. The length of the upper box 18 is the same as the depth of the inner cavity of the lower box 19, and the width of the upper box 18 is the same as the width of the inner cavity of the lower box 19. The upper box 18 has equally spaced upper cylinders 181 on its inner side, and the lower box 19 has equally spaced lower cylinders 191 on its inner side. Both the upper cylinders 181 and the lower cylinders 191 are cylindrical transparent plastic parts. The outer diameter of the upper cylinder 181 is the same as the inner diameter of the lower cylinder 191, and the upper cylinders 181 and lower cylinders 191 correspond one-to-one. The upper box 18 has a through hole 11 communicating with the inner cavity of the upper cylinder 181 at its top, and the lower box 19 has a through hole 11 communicating with the inner cavity of the lower cylinder 191 at its bottom. After the upper box 18 is inserted into the lower box 19, the upper cylinder 181 extends into the corresponding lower cylinder 191, and the inner cavities of the upper cylinder 181 and the lower cylinder 191 together form a planting cavity. The friction between the upper box 18 and the lower box 19 ensures that they will not move on their own. A seed holder 13 is provided inside the upper cylinder 181. Figure 17 As shown, by adjusting the relative position between the upper box 18 and the lower box 19, the height of the entire seed strip 1 can be adjusted to meet the support needs of seedlings of different heights.

[0024] like Figure 7 As shown, the main body of the basket 2 is rectangular. Handles 21 are located on both the left and right side walls of the basket 2 for easy gripping. The upper surface of the basket 2 has partition plates 22, which divide the space above the basket 2 into two areas. Within each area, several equally spaced clamping partitions 23 are fixed. A placement groove 25 is formed between every two adjacent clamping partitions 23. The width of the placement groove 25 is the same as the width of the seed strip 1, and the placement groove 25 is used to place the seed strip 1. Figure 8 As shown, the basket 2 has several bottom holes 24, and the bottom holes 24 correspond one-to-one with the placement grooves 25 and are connected.

[0025] like Figure 9 As shown, during use, the seed strip 1 is inserted into the placement slot 25 on the basket 2, at which point the seed strip 1 and the basket 2 are relatively fixed. When the middle of the seed strip 1 is corrugated, to simplify the structure, a fixed-length mechanism may not be provided. The width of the upper and lower ends of the seed strip 1 is designed to be greater than the width of the corrugated part 12. Laterally oriented slots are provided on the side wall of the clamping partition 23 of the basket 2, and multiple slots are evenly spaced from top to bottom. The width of the slots is the same as the thickness of the upper and lower ends of the seed strip 1. During use, the upper end of the seed strip 1 is inserted into different slots according to the height of the seedling, so that the height of the seed strip 1 matches the seedling.

[0026] The following describes the method of using the identification device of the present invention: S1. Place the seed in the planting cavity of the seed strip 1 in its contracted state, and support the seed with the seed holder 13. In the early stage of seed emergence, the young stem has weak support, and the planting cavity of the seed strip 1 in its contracted state can provide auxiliary support for the young stem, preventing the newly emerged seed from falling over. S2. As... Figure 9 As shown, insert the seed strip 1 into the placement slot 25 of the basket 2. S3, as... Figure 10 As shown, a basket 2 containing seed strip 1 is placed inside a culture box 3, which contains nutrient solution or saline solution required for the experiment. The nutrient solution or saline solution is absorbed by the roots after passing through the bottom hole 24 and the through hole 11. Once the seeds have emerged and are no longer prone to lodging, the seed strip 1 is stretched according to the seedling height. At this time, the seedling is in the seedling growth zone D1, and the roots pass through the seed holder 13 and enter the root growth zone D2.

[0027] This invention provides support for young stems by placing seeds within the planting chambers of seed strips, preventing them from collapsing due to their thinness and weakness. The expandable design of the seed strips accommodates the support needs of seedlings of varying heights, thus meeting the growth requirements of seeds at different stages of emergence and seedling development. The transparent structure of the seed strips allows for easy observation of individual seed emergence and seedling growth by placing different seeds in different planting chambers. This enables 360-degree in-situ, non-destructive observation of root development and stem / leaf growth for each seed, meeting the needs of molecular breeding for precise phenotypic data collection. The basket design allows for uniform assembly of the seed strips. Placing the basket within the culture box ensures uniform stress on seeds within different seed strips, guaranteeing consistent treatment conditions for each individual plant and significantly improving the accuracy, repeatability, and efficiency of salt tolerance identification.

Claims

1. A device for identifying salt tolerance of legume crops at seedling stage for easy observation, characterized in that, The application relates to a seedling strip, a basket and a culture box, wherein the seedling strip is a telescopic transparent piece, the seedling strip is provided with a planting cavity, the planting cavity is provided with a seed holder with holes, the basket is provided with a placing groove, the bottom of the basket is provided with a bottom hole communicating with the placing groove, and the seedling strip is placed in the placing groove after the seedling strip is placed in the planting cavity, then the basket is placed in the culture box containing nutrient solution or salt water.

2. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The seedling strip is a whole cuboid, the upper end and the lower end of the seedling strip are hard structures, and the middle part of the seedling strip is a corrugated part.

3. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 2, wherein, The upper end and the lower end of the seedling strip are provided with through holes, the corrugated part of the seedling strip is provided with a telescopic cylinder, the top of the telescopic cylinder is fixedly connected with the upper end of the seedling strip, the bottom of the telescopic cylinder is fixedly connected with the lower end of the seedling strip, and the through hole and the inner cavity of the telescopic cylinder form the planting cavity.

4. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The left and right ends of the seedling strip are provided with a size fixing mechanism, the size fixing mechanism comprises a screw rod and a nut, the screw rod is fixedly connected with the lower end of the seedling strip, the screw rod is arranged on the upper end of the seedling strip and extends out of the seedling strip, the nut is arranged on the screw rod extending out of the seedling strip, and the nut is in contact with the upper end of the seedling strip.

5. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The left and right ends of the seedling strip are provided with a size fixing mechanism, the size fixing mechanism comprises a shaped fixing clamp, and the seedling strip is arranged on the inner side of the fixing clamp.

6. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The middle part of the seedling strip is provided with a size fixing mechanism, the size fixing mechanism comprises a shaped fixing clamp, and the seedling strip is arranged on the inner side of the fixing clamp.

7. The device for identifying salt tolerance of legume crops at seedling stage for easy observation according to claims 5 and 6, characterized in that, The upper end surface and the lower end surface of the seedling strip are provided with grooves, and the fixing clamp extends into the grooves.

8. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The seedling strip comprises an upper box and a lower box, the bottom of the upper box is open, the top of the lower box is open, the upper box is provided with an upper cylinder, the lower box is provided with a lower cylinder, the length and width of the upper box are the same as the cross section size of the inner cavity of the lower box, the inner cavities of the upper cylinder and the lower cylinder form the planting cavity, the upper cylinder extends into the lower cylinder after the upper box is inserted into the lower box, and the seed holder is arranged in the upper cylinder.

9. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The upper surface of the basket is provided with a partition plate, the upper surface of the basket is divided into two areas by the partition plate, a plurality of clamping partition plates are arranged in each area at equal intervals, and the placing groove is formed between each two adjacent clamping partition plates.

10. The device for easy observation of salt tolerance of legume crops at seedling stage as claimed in claim 1, wherein, The seed holder is a cross-shaped, rice-shaped or net-shaped structure.

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