An anti-stress yield-increasing plant growth regulator, and a preparation method and application thereof

Abstract

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulator technology, specifically relating to a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application. Background Technology

[0002] With increasingly scarce global arable land resources, soil salinization has become a significant environmental factor restricting agricultural production. Salt stress leads to increased soil osmotic pressure and exacerbated ion toxicity, severely inhibiting crop root development and water and nutrient absorption. It also triggers the accumulation of reactive oxygen species and membrane lipid peroxidation, ultimately resulting in stunted plant growth, decreased photosynthetic efficiency, and a significant reduction in yield. Traditional methods for improving saline-alkali land, such as water conservancy projects and chemical amendments, are often costly, time-consuming, and difficult to achieve rapid results during the crop growing season.

[0003] Currently, while single or simply compounded plant growth regulators can alleviate abiotic stress to some extent, most products have limited functionality, often focusing on a single physiological process (such as root promotion or antioxidant effects). In practice on saline-alkali land, they are difficult to synergistically achieve the dual goals of "stress resistance and survival" and "stable yield and increased income." Especially for staple crops such as wheat, yield formation is easily inhibited under saline-alkali conditions, urgently requiring a compound regulator that can systematically regulate multiple stress resistance pathways while simultaneously enhancing photosynthesis and yield traits.

[0004] In recent years, the use of natural plant extracts and other ingredients has gradually attracted attention. Suaeda salsa, as a typical halophyte, is rich in osmotic regulators and antioxidants, but its effects are limited when used alone. Meanwhile, signaling substances such as brassinolide and crocin play a core role in regulating growth and stress response. How to achieve functional complementarity and synergistic effects among the components through scientific formulation has become the key to developing highly efficient stress-resistant and yield-increasing products.

[0005] Against this backdrop, this invention integrates multiple substances such as active extracts of Suaeda salsa, propionyl brassinolide, crocin, and perilla lactone to construct a multi-pathway synergistic compound regulator. It aims to simultaneously activate the antioxidant defense, osmotic regulation, photosynthetic promotion, and systemic stress resistance signaling network in crops, thereby not only ensuring the normal physiological functions of wheat under saline-alkali stress but also significantly enhancing its yield potential, providing an effective solution for the sustainable development of agriculture in saline-alkali land. Summary of the Invention

[0006] The purpose of this invention is to provide a stress-resistant and yield-increasing plant growth regulator that improves the stress resistance of wheat in saline-alkali land, promotes wheat growth, and increases wheat yield.

[0007] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0008] A stress-resistant and yield-increasing plant growth regulator comprises the following raw materials in the indicated weight percentages: 0.04-0.06% Suaeda salsa extract, 0.01-0.02% stress-resistant biostimulant, 0.001-0.002% propionyl brassinolide, 0.01-0.05% cymoxanil, 0.5-2% polyaspartic acid, 0.8-1% γ-aminobutyric acid, 3-8% emulsifier, 2-5% penetrant, 1-2% co-emulsifier, 0.1-0.3% pH buffer, and deionized water to 100%.

[0009] Furthermore, the preparation method of the Suaeda salsa extract is as follows:

[0010] (1) Remove impurities from fresh Suaeda salsa stems and leaves, wash with water, dry in an oven at 40-50℃ until the moisture content is <8%, and pulverize through a 40-60 mesh sieve to obtain Suaeda salsa powder;

[0011] (2) Mix Suaeda salsa powder and 0.5-1 mol / L citric acid aqueous solution at a mass-volume ratio of 1 g: 15 mL and stir evenly; heat to 80-90℃ and extract at a constant temperature twice, each time for 1.5-2 h. During the extraction process, add 1-2% anhydrous sodium sulfate of the total mass of Suaeda salsa powder and citric acid aqueous solution; filter after the first extraction, add an equal amount of water to the filter residue for a second extraction, and obtain Suaeda salsa aqueous extract from the filtrate of the two extractions.

[0012] (3) Pour the water extract of Suaeda salsa into a rotary evaporator and concentrate it to 1 / 5 of the original volume to obtain a concentrated solution. Add anhydrous ethanol to the concentrated solution to make the volume fraction of ethanol in the system reach 60%. Let it stand at 4℃ for 12-24 hours, filter it with a filter press, and collect the filtrate.

[0013] (4) The filtrate was concentrated again using a rotary evaporator to obtain a thick paste concentrate, which was then freeze-dried or spray-dried under vacuum to obtain Suaeda salsa extract.

[0014] Furthermore, the stress-relief biostimulant includes perillaldehyde.

[0015] Furthermore, the emulsifier is fatty alcohol polyoxyethylene ether.

[0016] Furthermore, the penetrant is sodium lignosulfonate.

[0017] Furthermore, the co-emulsifier is propylene glycol.

[0018] A method for preparing a stress-resistant and yield-increasing plant growth regulator includes the following steps:

[0019] First, heat deionized water to 40-50℃, add polyaspartic acid and γ-aminobutyric acid, and stir until completely dissolved; then add emulsifier and co-emulsifier, and stir evenly to form an aqueous phase; then dissolve propionyl brassinolide, crotonin, and stress-resistant biostimulant in ethanol, and slowly add them to the aqueous phase, shear at 3000-5000 r / min for 20 minutes; finally, add Suaeda salsa extract and penetrant, stir evenly, cool to room temperature, and add pH buffer to obtain the final product, plant growth regulator.

[0020] The present invention also provides the application of the stress-resistant and yield-increasing plant growth regulator, specifically for improving the stress resistance of crops in saline-alkali land and increasing yield.

[0021] Furthermore, the crop in question is wheat.

[0022] All raw materials used in this invention are commercially available.

[0023] Perilla lactone can activate the antioxidant system, enhance the activity of key enzymes such as SOD and POD, strengthen the cell's ability to scavenge ROS from the source, and reduce membrane lipid peroxidation. Reducing membrane lipid peroxidation can maintain the integrity and stability of the cell membrane, thereby ensuring the normal physiological function of root cells, promoting root elongation, and improving root water absorption capacity. It can also stabilize chloroplast structure, increase photosynthetic rate and chlorophyll content, and alleviate photosynthetic inhibition under salt stress.

[0024] Suaeda salsa is a typical salt-tolerant plant. It synthesizes and accumulates osmotic regulators such as betaine, proline, and soluble sugars, which help wheat cells maintain osmotic potential, alleviate cell dehydration caused by salt and alkali stress, enhance root water absorption, and reduce the damage to cell structure caused by ion toxicity. Suaeda salsa extract works synergistically with perillaldehyde in the formula to further enhance the activity of antioxidant enzymes such as SOD and POD in wheat. A large number of antioxidants synthesized by Suaeda salsa itself (such as flavonoids and polyphenols) are present in the extract, which can directly act as antioxidants to assist the endogenous system in scavenging reactive oxygen species and reducing membrane lipid peroxidation. The synergistic effect of Suaeda salsa extract and polyaspartic acid in the formula is enhanced: on the one hand, it alleviates root damage caused by salt and alkali, promotes root elongation and root hair development; on the other hand, it supplements nutrient absorption, improving the root system's absorption efficiency of nitrogen, phosphorus, potassium, and other nutrients in the soil, providing a material basis for wheat growth and yield formation.

[0025] Propionyl brassinolide effectively promotes cell division and elongation, accelerates the repair of damaged tissues and the growth of stems and leaves, and shortens the recovery period after stress; it also significantly improves the photosynthetic efficiency of leaves, promotes the transport and accumulation of photosynthetic products to grains, and increases yield.

[0026] Crown-like substance: As a "coordinating and amplifying signal of systemic stress response," it enhances the systemic and coordinated nature of plant adaptive responses. It can synergize with propionyl brassinolide to balance the allocation of growth and defense resources, and significantly improve the plant's response sensitivity and efficiency to other stress-resistant active substances in the entire formula (such as Suaeda salsa extract and sage lactone), making the compound's effect faster and more lasting.

[0027] Polyaspartic acid, with its high molecular weight properties, can chelate soil nutrients, improve the availability of nutrients in saline-alkali environments, and synergistically promote root absorption with Suaeda salsa extract; at the same time, it helps to improve the rhizosphere microenvironment and indirectly reduce the direct damage of salt ions to the roots.

[0028] Gamma-aminobutyric acid (GABA) is an important stress metabolite that acts as a "rapid osmotic regulator and metabolic buffer." It accumulates rapidly in the early stages of stress, participates in maintaining cellular osmotic pressure and pH homeostasis, provides the carbon and nitrogen skeleton, helps maintain basal energy metabolism, and provides metabolic substrate support for stress response.

[0029] Beneficial effects

[0030] The present invention combines the extract of Suaeda salsa with anti-stress active ingredients such as propionyl brassinolide, crocin, and sage lactone to form a synergistic anti-stress effect: it not only strengthens the core anti-stress pathways of anti-oxidation and osmotic regulation, but also enhances the tolerance of wheat in saline-alkali land in all aspects by promoting photosynthesis and anti-stress signal transduction, and finally achieves the dual effect of stress resistance and increased yield.

[0031] This invention's regulator is specifically designed for wheat grown in saline-alkali soils, offering highly targeted benefits. This formula not only significantly enhances wheat's tolerance to saline-alkali stress by strengthening its antioxidant system and osmotic regulation capabilities, ensuring its basic survival and normal physiological functions, but more importantly, it effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product transport to grains, thereby achieving a significant yield increase under adverse conditions. It simultaneously solves the problems of "survival" and "income increase" in saline-alkali land agriculture. Detailed Implementation

[0032] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.

[0033] Example 1

[0034] A stress-resistant and yield-increasing plant growth regulator comprises the following raw materials in the following weight percentages: 0.04% Suaeda salsa extract, 0.01% stress-resistant biostimulant, 0.001% propionyl brassinolide, 0.01% cymoxanil, 0.5% polyaspartic acid, 0.8% γ-aminobutyric acid, 3% emulsifier, 2% penetrant, 1% co-emulsifier, 0.1% pH buffer, and deionized water to make up to 100%.

[0035] The preparation method of the Suaeda salsa extract is as follows:

[0036] (1) Remove impurities from fresh Suaeda salsa stems and leaves, wash with water, dry in an oven at 40°C until the moisture content is <8%, and pulverize through a 40-60 mesh sieve to obtain Suaeda salsa powder;

[0037] (2) Mix Suaeda salsa powder and 0.5 mol / L citric acid aqueous solution at a mass-volume ratio of 1 g: 15 mL and stir evenly; heat to 90℃ and extract at a constant temperature twice, each time for 1.5 h. During the extraction process, add 1% anhydrous sodium sulfate of the total mass of Suaeda salsa powder and citric acid aqueous solution; filter after the first extraction, add an equal amount of water to the filter residue for a second extraction, and obtain Suaeda salsa aqueous extract from the filtrate of the two extractions.

[0038] (3) Pour the water extract of Suaeda salsa into a rotary evaporator and concentrate it to 1 / 5 of the original volume to obtain a concentrated solution. Add anhydrous ethanol to the concentrated solution to make the volume fraction of ethanol in the system reach 60%. Let it stand at 4℃ for 12 hours, filter it with a filter press, and collect the filtrate.

[0039] (4) The filtrate was concentrated again using a rotary evaporator to obtain a thick paste concentrate, which was then freeze-dried or spray-dried under vacuum to obtain Suaeda salsa extract.

[0040] The stress-relief biostimulant includes perillaldehyde.

[0041] The emulsifier is fatty alcohol polyoxyethylene ether.

[0042] The penetrant is sodium lignosulfonate.

[0043] The emulsifier is propylene glycol.

[0044] A method for preparing a stress-resistant and yield-increasing plant growth regulator includes the following steps:

[0045] First, deionized water is heated to 40°C, and polyaspartic acid and γ-aminobutyric acid are added and stirred until completely dissolved. Then, emulsifier and co-emulsifier are added and stirred evenly to form an aqueous phase. Next, propionyl brassinolide, crotonin, and stress-resistant biostimulant are dissolved in ethanol and slowly added to the aqueous phase. The mixture is sheared at 3000 r / min for 20 minutes. Finally, Suaeda salsa extract and penetrant are added, stirred evenly, cooled to room temperature, and then pH buffer is added to obtain the final product, a plant growth regulator.

[0046] Example 2

[0047] A stress-resistant and yield-increasing plant growth regulator comprises the following raw materials in the following weight percentages: 0.05% Suaeda salsa extract, 0.02% stress-resistant biostimulant, 0.002% propionyl brassinolide, 0.03% cymoxanil, 1.2% polyaspartic acid, 0.9% γ-aminobutyric acid, 5% emulsifier, 4% penetrant, 2% co-emulsifier, 0.2% pH buffer, and deionized water to make up to 100%.

[0048] The preparation method of the Suaeda salsa extract is as follows:

[0049] (1) Remove impurities from fresh Suaeda salsa stems and leaves, wash with water, dry in an oven at 45°C until the moisture content is <8%, and pulverize through a 40-60 mesh sieve to obtain Suaeda salsa powder;

[0050] (2) Mix Suaeda salsa powder and 1 mol / L citric acid aqueous solution at a mass-volume ratio of 1 g: 15 mL and stir evenly; heat to 85℃ and extract at a constant temperature twice, each time for 2 hours. During the extraction process, add 2% anhydrous sodium sulfate of the total mass of Suaeda salsa powder and citric acid aqueous solution; filter after the first extraction, add an equal amount of water to the filter residue for a second extraction, and obtain Suaeda salsa aqueous extract from the filtrate of the two extractions.

[0051] (3) Pour the water extract of Suaeda salsa into a rotary evaporator and concentrate it to 1 / 5 of the original volume to obtain a concentrated solution. Add anhydrous ethanol to the concentrated solution to make the volume fraction of ethanol in the system reach 60%. Let it stand at 4℃ for 18 hours, filter it with a filter press, and collect the filtrate.

[0052] (4) The filtrate was concentrated again using a rotary evaporator to obtain a thick paste concentrate, which was then freeze-dried or spray-dried under vacuum to obtain Suaeda salsa extract.

[0053] The stress-relief biostimulant includes perillaldehyde.

[0054] The emulsifier is fatty alcohol polyoxyethylene ether.

[0055] The penetrant is sodium lignosulfonate.

[0056] The emulsifier is propylene glycol.

[0057] A method for preparing a stress-resistant and yield-increasing plant growth regulator includes the following steps:

[0058] First, deionized water is heated to 45°C, and polyaspartic acid and γ-aminobutyric acid are added and stirred until completely dissolved. Then, emulsifier and co-emulsifier are added and stirred evenly to form an aqueous phase. Propionyl brassinolide, crotonin, and stress-resistant biostimulant are dissolved in ethanol and slowly added to the aqueous phase. The mixture is sheared at 4000 r / min for 20 minutes. Finally, Suaeda salsa extract and penetrant are added, stirred evenly, cooled to room temperature, and then pH buffer is added to obtain the final product, a plant growth regulator.

[0059] Example 3

[0060] A stress-resistant and yield-increasing plant growth regulator comprises the following raw materials in the following weight percentages: 0.06% Suaeda salsa extract, 0.02% stress-resistant biostimulant, 0.002% propionyl brassinolide, 0.05% cymoxanil, 2% polyaspartic acid, 1% γ-aminobutyric acid, 8% emulsifier, 5% penetrant, 2% co-emulsifier, 0.3% pH buffer, and deionized water to make up to 100%.

[0061] The preparation method of the Suaeda salsa extract is as follows:

[0062] (1) Remove impurities from fresh Suaeda salsa stems and leaves, wash with water, dry in an oven at 50°C until the moisture content is <8%, and pulverize through a 40-60 mesh sieve to obtain Suaeda salsa powder;

[0063] (2) Mix Suaeda salsa powder and 1 mol / L citric acid aqueous solution at a mass-volume ratio of 1 g: 15 mL and stir evenly; heat to 80℃ and extract at a constant temperature twice, each time for 2 hours. During the extraction process, add 2% anhydrous sodium sulfate of the total mass of Suaeda salsa powder and citric acid aqueous solution; filter after the first extraction, add an equal amount of water to the filter residue for a second extraction, and obtain Suaeda salsa aqueous extract from the filtrate of the two extractions.

[0064] (3) Pour the water extract of Suaeda salsa into a rotary evaporator and concentrate it to 1 / 5 of the original volume to obtain a concentrated solution. Add anhydrous ethanol to the concentrated solution to make the volume fraction of ethanol in the system reach 60%. Let it stand at 4℃ for 24 hours, filter it with a filter press, and collect the filtrate.

[0065] (4) The filtrate was concentrated again using a rotary evaporator to obtain a thick paste concentrate, which was then freeze-dried or spray-dried under vacuum to obtain Suaeda salsa extract.

[0066] The stress-relief biostimulant includes perillaldehyde.

[0067] The emulsifier is fatty alcohol polyoxyethylene ether.

[0068] The penetrant is sodium lignosulfonate.

[0069] The emulsifier is propylene glycol.

[0070] A method for preparing a stress-resistant and yield-increasing plant growth regulator includes the following steps:

[0071] First, deionized water is heated to 50°C, and polyaspartic acid and γ-aminobutyric acid are added and stirred until completely dissolved. Then, emulsifier and co-emulsifier are added and stirred evenly to form an aqueous phase. Next, propionyl brassinolide, crotonin, and stress-resistant biostimulant are dissolved in ethanol and slowly added to the aqueous phase. The mixture is sheared at 5000 r / min for 20 minutes. Finally, Suaeda salsa extract and penetrant are added, stirred evenly, cooled to room temperature, and then pH buffer is added to obtain the final product, a plant growth regulator.

[0072] Comparative Example 1

[0073] Compared with Example 3, this comparative example is identical to Example 3 except that no Suaeda salsa extract was added. All other raw materials and steps are the same as in Example 3.

[0074] Comparative Example 2

[0075] Compared with Example 3, this comparative example is identical to Example 3 except that the anti-stress biostimulant perillyl lactone is not added.

[0076] Performance testing

[0077] The wheat variety tested was “Xinchun 38”. A pot experiment was conducted on saline-alkali soil in the Yellow River Delta coastal area. The soil physicochemical properties were: pH 7.87, electrical conductivity (EC) 6.5 dS / m, available nitrogen 63.4 mg / kg, available phosphorus 54.7 mg / kg, and available potassium 119.5 mg / kg. The pot dimensions were 50cm (length) × 19cm (width) × 15cm (height).

[0078] The experiment consisted of six treatment groups: a control group (CK, water) without plant growth regulators; and control groups treated with plant growth regulators prepared in Examples 1-3 and Comparative Examples 1-2, diluted 400 times. Each treatment was repeated three times. Plump, uniform-sized seeds free from pests and diseases were selected. The seeds underwent 7 days of low-temperature vernalization, followed by transplanting the wheat into saline-alkali soil. When the wheat reached the two-leaf stage, the plant growth regulators for each treatment group were sprayed until water droplets dripped from the leaves. A total of four sprays were applied, with a 3-day interval between each application. All other management practices were the same as in the field.

[0079] Index Measurement: Two days after the final application of the plant growth regulator, samples were taken from each treatment group during the wheat seedling stage. One sample was used to measure plant height, root length, and leaf fresh weight; the other sample was immediately placed in an ultra-low temperature freezer at -80℃ for subsequent measurement of various antioxidant enzyme activities. Superoxide dismutase (SOD) was measured using the nitroblue tetrazolium method, and peroxidase (POD) was measured using the guaiacol method. Additionally, malondialdehyde (MDA) content was determined using the thiobarbituric acid reaction method. These data are shown in Table 1. After the wheat matured, the number of wheat ears per pot was measured, and the number of grains per ear from 10 randomly selected plants was measured. The wheat in the pots was harvested, dried, and the thousand-grain weight was measured to calculate the yield. These data are shown in Table 2.

[0080] Table 1 Results of wheat plant growth and stress resistance indices for each treatment.

[0081]

[0082] As shown in Table 1, compared with the control group (CK), the wheat plants sprayed with the plant growth regulator of this invention exhibited significantly increased plant height and root length, indicating that the plant growth regulator of this invention can significantly improve the growth of wheat plants on saline-alkali land. Plants can resist reactive oxygen species damage by increasing the activity of antioxidant enzymes. The measured antioxidant enzyme activities showed a significant increase in SOD and POD activities, indicating that the plant growth regulator of this invention can improve the antioxidant capacity of wheat plants. Furthermore, the MDA data showed that, compared with the blank control group, the MDA content in wheat plants after applying the plant growth regulator of this invention was much lower than that in the CK group. MDA content reflects the level of lipid peroxidation of plant membranes and the degree of cell membrane damage; the higher the content, the greater the degree of membrane damage. The results of this study indicate that the application of the plant growth regulator of this invention can reduce the MDA content of wheat plants, improve the stress resistance of wheat plants, and reduce the degree of membrane damage. In summary, the above data show that the plant growth regulator of this invention can significantly improve the salt and alkali resistance of wheat and effectively promote plant growth.

[0083] Table 2. Wheat and its constituent yield factors in each treatment group

[0084]

[0085] As shown in Table 2, compared with the control group, the use of the plant growth regulator of the present invention significantly increased the number of ears, number of grains per ear, thousand-grain weight and theoretical yield of wheat at the harvest period. This indicates that the plant growth regulator of the present invention can significantly improve the salt and alkali resistance of wheat plants, thereby improving the absorption of nutrients by wheat plants and promoting plant growth and yield formation.

[0086] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. A stress-resistant and yield-increasing plant growth regulator, characterized in that, The raw materials include the following percentages by weight: Suaeda salsa extract 0.04-0.06%, anti-stress biostimulant 0.01-0.02%, propionyl brassinolide 0.001-0.002%, crotonin 0.01-0.05%, polyaspartic acid 0.5-2%, γ-aminobutyric acid 0.8-1%, emulsifier 3-8%, penetrant 2-5%, co-emulsifier 1-2%, pH buffer 0.1-0.3%, and deionized water to 100%. The stress-relief biostimulant is perilla lactone; the emulsifier is fatty alcohol polyoxyethylene ether; the penetrant is sodium lignosulfonate; and the co-emulsifier is propylene glycol.

2. The stress-resistant and yield-increasing plant growth regulator according to claim 1, characterized in that, The preparation method of the Suaeda salsa extract is as follows: (1) Remove impurities from fresh Suaeda salsa stems and leaves, wash with water, dry in an oven at 40-50℃ until the moisture content is <8%, and pulverize through a 40-60 mesh sieve to obtain Suaeda salsa powder; (2) Mix Suaeda salsa powder and 0.5-1 mol / L citric acid aqueous solution at a mass-volume ratio of 1 g: 15 mL and stir evenly; heat to 80-90℃ and extract at a constant temperature twice, each time for 1.5-2 h. During the extraction process, add 1-2% anhydrous sodium sulfate of the total mass of Suaeda salsa powder and citric acid aqueous solution; filter after the first extraction, add an equal amount of water to the filter residue for a second extraction, and obtain Suaeda salsa aqueous extract from the filtrate of the two extractions. (3) Pour the water extract of Suaeda salsa into a rotary evaporator and concentrate it to 1 / 5 of the original volume to obtain a concentrated solution. Add anhydrous ethanol to the concentrated solution to make the volume fraction of ethanol in the system reach 60%. Let it stand at 4℃ for 12-24 hours, filter it with a filter press, and collect the filtrate. (4) The filtrate was concentrated again using a rotary evaporator to obtain a thick paste concentrate, which was then freeze-dried or spray-dried under vacuum to obtain Suaeda salsa extract.

3. A method for preparing the stress-resistant and yield-increasing plant growth regulator according to any one of claims 1-2, characterized in that, Preparation includes the following steps: First, heat deionized water to 40-50℃, add polyaspartic acid and γ-aminobutyric acid, and stir until completely dissolved; then add emulsifier and co-emulsifier, and stir evenly to form an aqueous phase; then dissolve propionyl brassinolide, crotonin, and stress-resistant biostimulant in ethanol, and slowly add them to the aqueous phase, shear at 3000-5000 r / min for 20 minutes; finally, add Suaeda salsa extract and penetrant, stir evenly, cool to room temperature, and then add pH buffer to obtain the final product, plant growth regulator.

4. The application of the stress-resistant and yield-increasing plant growth regulator according to claim 1, characterized in that, The specific application is to improve the crop's resistance to stress in saline-alkali land and increase crop yield.

5. The application according to claim 4, characterized in that, The crop in question is wheat.

Images