Compound enzyme preparation-containing nutritional feed for regulating intramuscular fat of black pigs and preparation method of compound enzyme preparation-containing nutritional feed

Abstract

The invention discloses a compound enzyme preparation-containing nutritional feed for regulating and controlling intramuscular fat of black pigs and a preparation method of the compound enzyme preparation-containing nutritional feed, and relates to the technical field of livestock and poultry breeding feeds. The invention discloses a black pig skeletal muscle cell fat directional transport feed which is prepared from the following components in parts by weight: 850-920 parts of basal feed, 5-12 parts of fat directional deposition compound enzyme group, 25-40 parts of amino acid-enzymatic synthesis substrate combination and 2-6 parts of lipolytic enzyme specific inhibitor. According to the black pig skeletal muscle cell fat directional transport feed, fat precursor directional transport is realized through the compounded fat directional deposition compound enzyme group, a fat synthesis pathway targeting black pig skeletal muscle cells and lipoprotein lipase; acetyl-coenzyme A carboxylase and fatty acid synthetase catalyze fat synthesis step by step, only intramuscular fat generation pathways are activated, subcutaneous and abdominal fat deposition paths are blocked, the technical defects that fat deposition of an existing single enzyme preparation is non-directional, and the body fat rate is caused to be abnormally increased are overcome, meanwhile, an exclusive amino acid-enzymatic synthesis substrate combination is matched, and the body fat synthesis efficiency is improved. Sufficient adaptive substrates are supplied to enzyme group catalytic reaction, and the problem that a single enzyme preparation is insufficient in catalytic efficiency is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of livestock and poultry breeding feed, in particular to a black pig intramuscular fat regulation nutrition feed containing a compound enzyme preparation and a preparation method thereof. BACKGROUND

[0002] Black pig meat has extremely high economic value due to the marbling pattern, excellent tenderness and unique flavor formed by its rich intramuscular fat (IMF). Intramuscular fat content is a core indicator for measuring pork quality. Currently, the main way to improve black pig IMF in the industry relies on genetic selection and later high-energy feed fattening. However, genetic improvement takes a long time, and traditional nutritional enhancement methods often lead to energy excess, which increases IMF while more easily causing excessive accumulation of subcutaneous and abdominal fat, resulting in decreased lean meat rate, reduced feed reward, and potential health risks, making it impossible to achieve precise unification of quality and efficiency.

[0003] In the prior art, feed additive research for improving meat quality has focused on single functional components. For example, adding general-purpose digestive enzymes aims to improve overall feed utilization, but its effect lacks tissue specificity and cannot distinguish between the metabolic pathways of intramuscular and subcutaneous fat, limiting the effectiveness and stability of targeted IMF improvement. Some studies have focused on functional amino acids, but they usually use conventional balanced addition mode, only serving as protein synthesis raw materials, and fail to establish a targeted "enzyme-substrate" synergy relationship with key enzymes in the fat synthesis metabolic pathway, making the nutrient flow unable to be precisely guided to the IMF deposition site. Moreover, these studies only focus on promoting synthesis, ignoring the importance of inhibiting decomposition, lack of functional components that can selectively stabilize synthesized intramuscular fat, resulting in poor deposition efficiency and easy loss of results due to metabolic fluctuations.

[0004] Therefore, there is an urgent need for a technology that can systematically solve the above bottlenecks. The purpose of the present application is to break through the traditional single and extensive nutritional regulation mode, design a multi-component synergistic nutritional strategy, and achieve precise intervention in the whole chain of black pig intramuscular fat metabolism from synthesis initiation, substrate supply to product stabilization, thereby efficiently and directionally optimizing meat quality traits while ensuring growth performance. SUMMARY

[0005] The present application aims at solving the problems in the prior art, and provides a black pig intramuscular fat regulation nutrition feed containing a complex enzyme preparation and a preparation method.

[0006] To solve the above technical problems, the present application provides the following technical scheme: on the one hand, a black pig intramuscular fat regulation nutrition feed containing a complex enzyme preparation, the black pig intramuscular fat regulation nutrition feed is composed of 850-920 parts by weight of a basic feed, 5-12 parts by weight of a fat directional deposition complex enzyme group, 25-40 parts by weight of an amino acid-enzymatic synthesis substrate combination, and 2-6 parts by weight of a lipase specific inhibitor. The fat directional deposition complex enzyme group is composed of lipoprotein lipase, acetyl-CoA carboxylase, and fatty acid synthase in a mass ratio of (2-3):(4-5):(2-4). The amino acid-enzymatic synthesis substrate combination is composed of leucine, isoleucine, glutamic acid, and alanine in a mass ratio of (3-5):(2-3):(1-2):(1-2). The lipase specific inhibitor is a natural tea polyphenol modified product.

[0007] Further, the basic feed is composed of 500-580 parts by weight of corn flour, 180-220 parts by weight of soybean meal, 60-80 parts by weight of bran, 40-60 parts by weight of secondary powder, 8-12 parts by weight of stone powder, 5-8 parts by weight of calcium hydrogen phosphate, and 3-5 parts by weight of salt.

[0008] Further, in the fat directional deposition complex enzyme group, the lipoprotein lipase has an enzyme activity of ≥2000 U / g, the acetyl-CoA carboxylase has an enzyme activity of ≥1500 U / g, and the fatty acid synthase has an enzyme activity of ≥1800 U / g.

[0009] Further, the leucine, isoleucine, glutamic acid, and alanine in the amino acid-enzymatic synthesis substrate combination are all food-grade L-type amino acids, and the purity of each amino acid is ≥99%.

[0010] Further, the preferred mass ratio of the fat directional deposition complex enzyme group is lipoprotein lipase:acetyl-CoA carboxylase:fatty acid synthase = 2:5:3. The preferred mass ratio of the amino acid-enzyme synthesis substrate combination is leucine: isoleucine: glutamic acid: alanine = 4:2:1:1.

[0011] Further, the feed product is in granular form, the moisture content is controlled at 10-12%, the particle hardness is 2.5-3.0 kg / cm2, and the particle size is 3-4 mm.

[0012] Further, the preparation process of the natural tea polyphenol modified product is as follows: The tea polyphenol and citric acid are mixed at a mass ratio of 5:1, then placed in a 60-65℃ water bath environment for constant temperature stirring at 200r / min for 2h, cooled to room temperature, and dried to a moisture content of ≤5%, and then pulverized to 100 mesh to obtain.

[0013] On the other hand, a method for preparing a black pig intramuscular fat regulation nutritional feed containing a complex enzyme preparation, the steps of the method are as follows: S100, the corn flour, soybean meal, bran, and second flour in the base feed are respectively pulverized to 80-100 mesh and sieved, and then uniformly mixed to obtain a base mixture, the stone powder, calcium hydrogen phosphate, and salt are ground to 120 mesh and sieved, and then uniformly mixed to obtain a mineral premix, which is ready for use; S200, weigh the lipoprotein lipase, acetyl coenzyme A carboxylase, and fatty acid synthase, and stir at 150-200r / min at room temperature for 10-15min, then uniformly mix to obtain a fat directional deposition complex enzyme group, which is sealed and stored away from light; S300, weigh the leucine, isoleucine, glutamic acid, and alanine, and stir at 120-150r / min at room temperature for 8-10min, then uniformly mix to obtain an amino acid-enzyme synthesis substrate combination, which is used to prepare a lipase-specific inhibitor, which is ready for use; S400, put the base mixture and the mineral premix of S100 into a horizontal mixer, and stir at 250-300r / min for 20-25min to obtain a base material, then add the fat directional deposition complex enzyme group and the amino acid-enzyme synthesis substrate combination to the base material, and stir at 200-250r / min for 15-20min, then add the lipase-specific inhibitor, and stir at 180-200r / min for 10-12min, then mix until no obvious granular material is observed, and obtain a mixture; S500, send the mixture into a granulator, control the granulation temperature at 70-75℃, and control the ring die aperture at 3-4mm to obtain a granular feed, then cool the granular feed to room temperature using a cooler, screen out the broken material, and obtain a finished feed.

[0014] Further, in the S200, when preparing the fat directional deposition complex enzyme group, the stirring environment is a light-proof and dry environment, the environmental humidity is less than or equal to 45%, and the storage temperature of the prepared complex enzyme group under sealed light-proof conditions is 2-8 DEG C.

[0015] Compared with the prior art, the black pig intramuscular fat regulation nutritional feed containing a complex enzyme preparation and the preparation method have the following beneficial effects: Firstly, the fat directional deposition complex enzyme group of the present application can precisely target the fat synthesis pathway of black pig skeletal muscle cells, the lipoprotein lipase realizes directional transport of fat precursors, the acetyl coenzyme A carboxylase and the fatty acid synthase catalyze fat synthesis step by step, only activate the intramuscular fat generation pathway, and block the subcutaneous and abdominal fat deposition pathways, thereby solving the technical defects of the prior art that the fat deposition of the single enzyme preparation is not directional and causes abnormal increase of body fat rate.

[0016] Secondly, the present application relies on the synergistic effect of the amino acid-enzymatic synthesis substrate combination and the specific inhibitor of lipase, on the one hand, the branched-chain amino acid ensures the raw materials required for skeletal muscle growth, and glutamic acid and alanine are simultaneously used as fat synthesis substrates, so that the muscle growth and intramuscular fat synthesis are promoted simultaneously, and the problem of antagonism between muscle fat deposition and lean meat rate caused by the addition of single amino acid is solved.

[0017] Other advantages, objects, and features of the present application will be set forth in part in the following specification taken in conjunction with the accompanying drawings, and in part will become apparent to those skilled in the art from a consideration of the following specification and drawings, or can be learned from the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0019] Figure 1 The flow chart of the preparation method of the black pig intramuscular fat regulation nutritional feed containing a complex enzyme preparation. DETAILED DESCRIPTION

[0020] In order to further illustrate the technical means and effects adopted by the present application to achieve the predetermined object of the present application, the specific embodiments, structures, features and effects according to the present application are described in detail below in combination with the drawings and preferred embodiments.

[0021] In order to improve the problems of non-directional fat deposition, insufficient enzyme and substrate cooperation, and poor intramuscular fat retention of existing black pig intramuscular fat regulation feed, first, the application scenarios involved in the present application are described. The present application is mainly applied to the scenes of livestock and poultry feed with high requirements for intramuscular fat directional deposition, lean meat rate guarantee and meat quality and flavor improvement in black pig fattening breeding. In these scenes, the traditional feed has problems such as low efficiency of intramuscular fat synthesis, excessive accumulation of subcutaneous and abdominal fat, and mutual antagonism between muscle fat deposition and lean meat rate, which seriously affects the meat quality and economic benefits of black pig breeding. The present application is designed and precisely matched by the synergistic effect of fat directional deposition complex enzyme group, amino acid-enzyme synthesis substrate combination and lipase specific inhibitor, aiming to realize the black pig special nutritional feed with intramuscular fat directional synthesis, precise substrate supply and stable deposition of deposited fat.

[0022] The black pig intramuscular fat regulation nutritional feed containing a complex enzyme preparation consists of 850-920 parts by weight of base feed, 5-12 parts by weight of fat directional deposition complex enzyme group, 25-40 parts by weight of amino acid-enzyme synthesis substrate combination, and 2-6 parts by weight of lipase specific inhibitor; wherein the base feed consists of 500-580 parts by weight of corn flour, 180-220 parts by weight of soybean meal, 60-80 parts by weight of bran, 40-60 parts by weight of secondary powder, 8-12 parts by weight of stone powder, 5-8 parts by weight of calcium hydrogen phosphate, and 3-5 parts by weight of salt, providing basic energy and nutritional support for the growth of black pigs; the fat directional deposition complex enzyme group is compounded by lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid synthase in a mass ratio of (2-3):(4-5):(2-4), and the optimal ratio is 2:5:3, and the enzyme activities of the three enzymes are ≥2000 U / g, ≥1500 U / g and ≥1800 U / g respectively, achieving directional synthesis of intramuscular fat; the amino acid-enzyme synthesis substrate combination is compounded by leucine, isoleucine, glutamic acid and alanine in a mass ratio of (3-5):(2-3):(1-2):(1-2), and the optimal ratio is 4:2:1:1, each amino acid is a food-grade L-amino acid with a purity of ≥99%, providing suitable substrates for fat synthesis and ensuring muscle growth; the lipase specific inhibitor is a natural tea polyphenol modified product, which is prepared by mixing tea polyphenol and citric acid in a mass ratio of 5:1, stirring at 60-65℃ for 2h, cooling and drying to a water content of ≤5%, and crushing to 100 mesh, which can selectively inhibit intramuscular fat decomposition; the feed product is in the form of granules, with a water content of 10-12%, a particle hardness of 2.5-3.0 kg / cm2, and a particle size of 3-4 mm, which is suitable for the feeding and digestion characteristics of black pigs.

[0023] Corresponding to the above black pig intramuscular fat regulation nutrition feed, the application also provides a preparation method of the black pig intramuscular fat regulation nutrition feed containing the composite enzyme preparation, such as Figure 1 As shown in the preparation method of the black pig intramuscular fat regulation nutrition feed containing the composite enzyme preparation, the specific steps are as follows: S100, the corn flour, soybean meal, bran and second flour in the basic feed are respectively crushed to 80-100 meshes and sieved, and then uniformly mixed to obtain a basic mixture, the stone powder, calcium hydrogen phosphate and salt are ground to 120 meshes and sieved, and then uniformly mixed to obtain a mineral premix, which is ready for use; S200, the lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid synthase are weighed, stirred at 150-200 r / min at room temperature for 10-15 min, uniformly mixed to obtain a fat directional deposition composite enzyme group, and then sealed and stored away from light; S300, the leucine, isoleucine, glutamic acid and alanine are weighed, stirred at 120-150 r / min at room temperature for 8-10 min, uniformly mixed to obtain an amino acid-enzyme synthesis substrate combination, a lipase specific inhibitor is prepared, and then ready for use; S400, the basic mixture and the mineral premix of S100 are put into a horizontal mixer, stirred at 250-300 r / min for 20-25 min to obtain a basic material, the fat directional deposition composite enzyme group and the amino acid-enzyme synthesis substrate combination are added to the basic material, stirred at 200-250 r / min for 15-20 min, and then the lipase specific inhibitor is added, stirred at 180-200 r / min for 10-12 min, mixed until no obvious granular material is observed, and then a mixture is obtained; S500, the mixture is sent into a granulator, the granulation temperature is controlled at 70-75 DEG C, the ring die aperture is 3-4 mm, and then granular feed is prepared, after cooling to room temperature by a cooler, the broken material is removed by screening, and then finished feed is obtained.

[0024] Example one The present embodiment provides a black pig intramuscular fat regulation nutrition feed containing a composite enzyme preparation and a preparation process thereof, the feed is compounded with a fat directional deposition composite enzyme group, an amino acid-enzyme synthesis substrate combination and a lipase specific inhibitor, and is prepared through detailed process steps, which aims to verify the feasibility and synergistic effect of the technical scheme of the application in the directional improvement of the intramuscular fat content of black pigs.

[0025] Raw material preparation: Basic feed: 550 kg of corn flour, 200 kg of soybean meal, 70 kg of bran, 50 kg of second flour, 10 kg of stone powder, 6.5 kg of calcium hydrogen phosphate and 4 kg of salt.

[0026] Lipid-directed deposition complex enzyme group: weigh 2.0 kg of lipoprotein lipase with enzyme activity ≥2000 U / g, 5.0 kg of acetyl-CoA carboxylase with enzyme activity ≥1500 U / g, and 3.0 kg of fatty acid synthase with enzyme activity ≥1800 U / g (mass ratio 2:5:3).

[0027] Amino acid-enzymatic synthesis substrate combination: weigh 16 kg of food-grade L-leucine with purity ≥99%, 8 kg of L-isoleucine, 4 kg of L-glutamic acid, and 4 kg of L-alanine (mass ratio 4:2:1:1).

[0028] Lipase-specific inhibitor: mix 5 kg of tea polyphenols with 1 kg of citric acid, and stir in a 62°C water bath at 200 r / min for 2 hours. After the reaction is cooled to room temperature, dry it to moisture ≤5%, and crush it to 100 mesh to obtain about 5.8 kg of natural tea polyphenol modified product. From it, weigh 4.0 kg for standby.

[0029] Preparation method: S100: crush corn meal, soybean meal, bran, and second flour to 90 mesh and sieve, mix uniformly to obtain a basic mixture. Grind stone powder, calcium hydrogen phosphate, and salt to 120 mesh and sieve, mix uniformly to obtain a mineral premix.

[0030] S200: in a light-proof dry environment with ambient humidity ≤45%, put the weighed lipoprotein lipase, acetyl-CoA carboxylase, and fatty acid synthase into a mixer, stir at 180 r / min for 12 minutes, mix uniformly to obtain a lipid-directed deposition complex enzyme group, immediately put it into a sealed light-proof container, and store at 4°C for standby.

[0031] S300: put the weighed leucine, isoleucine, glutamic acid, and alanine into a mixer, stir at 135 r / min for 9 minutes, mix uniformly to obtain an amino acid-enzymatic synthesis substrate combination.

[0032] S400: put the basic mixture obtained in S100 and the mineral premix into a horizontal mixer, stir at 280 r / min for 22 minutes to obtain a uniform basic material. Add the lipid-directed deposition complex enzyme group (10.0 kg) prepared in S200 and the amino acid-enzymatic synthesis substrate combination (32.0 kg) prepared in S300 to the basic material in turn, adjust the stirring speed of the mixer to 230 r / min, and stir for 18 minutes. Finally, add the prepared lipase-specific inhibitor (4.0 kg), and stir at 190 r / min for 11 minutes until the mixture is uniform and there is no obvious granular material.

[0033] S500: The mixture is sent to a granulator, and the granulation temperature is controlled at 72°C, and a compression die with a ring die aperture of 3.5 mm is used for granulation. The prepared pellet feed is cooled to room temperature (about 25°C) by a cooler, and then is screened by a grading screen to remove powder and crushed material, and finally the finished feed with a moisture content of about 11% and a pellet hardness of about 2.8 kg / cm2 is obtained, and the total amount is about 941.5 kg.

[0034] Example Two This example aims to verify the effect of using the fat directional deposition complex enzyme group alone. The feed formula and preparation process thereof are basically the same as those in Example One, but only the enzyme group is added, and the amino acid-enzymatic synthesis substrate combination and the lipase-specific inhibitor are not added. The total amount of the base feed remains unchanged, and the weight of the missing components is supplemented by an equal amount of bran.

[0035] Only the base feed (composition same as Example One) and the fat directional deposition complex enzyme group (10.0 kg, same ratio as Example One) are included in the formula. The amino acid combination and the inhibitor are not added, and the excess of the formula (32.0 kg+4.0 kg=36.0 kg) is supplemented by bran, and during preparation, only the enzyme group is added to the base material body for mixing in S400. The remaining preparation steps and parameters are the same as those in Example One.

[0036] Example Three This example aims to verify the effect of using the amino acid-enzymatic synthesis substrate combination alone. The feed formula and preparation process thereof are basically the same as those in Example One, but only the amino acid combination is added, and the fat directional deposition complex enzyme group and the lipase-specific inhibitor are not added. The total amount of the base feed remains unchanged, and the weight of the missing components is supplemented by an equal amount of bran.

[0037] Only the base feed (composition same as Example One) and the amino acid-enzymatic synthesis substrate combination (32.0 kg, same ratio as Example One) are included in the formula. The enzyme group and the inhibitor are not added, and the excess of the formula (10.0 kg+4.0 kg=14.0 kg) is supplemented by bran, and during preparation, only the amino acid combination is added to the base material body for mixing in S400, and S200 is omitted, and the remaining preparation steps and parameters are the same as those in Example One.

[0038] Example Four

[0039] This example aims to verify the effect of using the lipase-specific inhibitor alone. The feed formula and preparation process thereof are basically the same as those in Example One, but only the inhibitor is added, and the fat directional deposition complex enzyme group and the amino acid-enzymatic synthesis substrate combination are not added. The total amount of the base feed remains unchanged, and the weight of the missing components is supplemented by an equal amount of bran.

[0040] The formula contains only basal feed (same composition as in Example One) and lipase-specific inhibitor (4.0 kg, same as in Example One), without adding enzyme group and amino acid group combination, the excess of the formula (10.0 kg + 32.0 kg = 42.0 kg) is made up by bran, during preparation, only the inhibitor is added to the basal material body in S400 for mixing. S200 and S300 are omitted, and the rest of the preparation steps and parameters are the same as in Example One.

[0041] Example Five This example aims to verify the synergistic effect of fat targeted deposition complex enzyme group and amino acid-enzymatic synthesis substrate combination, the feed formula contains basal feed, enzyme group and amino acid combination, but does not contain lipase-specific inhibitor, the total amount of basal feed remains unchanged, and the weight of the inhibitor is supplemented by an equal amount of bran.

[0042] The formula contains basal feed (same composition as in Example One), fat targeted deposition complex enzyme group (10.0 kg) and amino acid-enzymatic synthesis substrate combination (32.0 kg), without adding inhibitor (4.0 kg), the weight is made up by bran, during preparation, the enzyme group and amino acid combination are added to the basal material body in S400 for mixing in turn, without adding inhibitor, the rest of the preparation steps and parameters are the same as in Example One.

[0043] Example Six This example aims to verify the synergistic effect of fat targeted deposition complex enzyme group and lipase-specific inhibitor, the feed formula contains basal feed, enzyme group and inhibitor, but does not contain specific amino acid-enzymatic synthesis substrate combination, the total amount of basal feed remains unchanged, and the weight of the amino acid combination is supplemented by an equal amount of ordinary soybean meal.

[0044] The formula contains basal feed (same composition as in Example One), fat targeted deposition complex enzyme group (10.0 kg) and lipase-specific inhibitor (4.0 kg), without adding specific amino acid combination (32.0 kg), the weight is made up by ordinary soybean meal, during preparation, the enzyme group and inhibitor are added to the basal material body in S400 for mixing in turn, S300 is omitted, and the rest of the preparation steps and parameters are the same as in Example One.

[0045] Example Seven This example aims to verify the synergistic effect of amino acid-enzymatic synthesis substrate combination and lipase-specific inhibitor, the feed formula contains basal feed, amino acid combination and inhibitor, but does not contain specific fat targeted deposition complex enzyme group, the total amount of basal feed remains unchanged, and the weight of the enzyme group is supplemented by an equal amount of bran.

[0046] The formula comprises base feed (same composition as in Example 1), amino acid-enzyme synthesis substrate combination (32.0 kg), and lipolytic enzyme specific inhibitor (4.0 kg), without adding specific enzyme group (10.0 kg), the weight of which is made up by bran, and when preparing, the amino acid combination and inhibitor are sequentially added to the base material in S400 and mixed, step S200, and the remaining preparation steps and parameters are the same as in Example 1.

[0047] Example Eight This example aims to verify the effect of using ordinary complex enzymes to replace the specific enzyme group of the present application, and the feed formula and preparation process are basically the same as in Example 1, but an equal amount of commercial ordinary complex enzyme (containing amylase, protease, lipase) is used to replace the fat directional deposition complex enzyme group.

[0048] The formula and preparation process are the same as in Example 1, except that the fat directional deposition complex enzyme group (10.0 kg) is replaced by a commercial general-purpose complex enzyme preparation with a total enzyme activity value of 10.0 kg (mainly containing amylase, neutral protease, and lipase). The purpose of adding this ordinary complex enzyme is to promote overall nutrient digestion, rather than directional regulation of fat synthesis. The preparation step S200 is changed to mix the ordinary complex enzyme, and the remaining steps are the same as in Example 1.

[0049] Comparative test Test animals and grouping: 224 healthy and well-conditioned black pig finishing pigs of the same strain with a body weight of (65±2) kg were randomly divided into 8 groups, 28 pigs per group, and were fed with the feed prepared in Examples 1 to 8.

[0050] Feeding management: The test period was 60 days, all test pigs were raised in the same pig house with controllable environment, free access to feed and water, and the same daily management and immunization program.

[0051] Test indicators: Growth performance: The total feed consumption was recorded by weighing the pigs empty stomach in the morning at the beginning and end of the test, and the average daily gain (ADG) and feed conversion ratio (F / G) were calculated.

[0052] Carcass traits: At the end of the test, 8 test pigs with close average body weight were randomly selected from each group for slaughter to determine the carcass backfat thickness (at the 6th-7th intercostal space) and eye muscle area.

[0053] Meat quality traits: The left carcass longissimus dorsi muscle sample was taken, and the intramuscular fat (IMF) content was determined by Soxhlet extraction method.

[0054] The experimental data were subjected to one-way ANOVA, and the results were expressed as mean ± standard deviation, with P<0.05 as the significant difference. According to the 60-day feeding experiment, the growth performance, carcass traits and meat quality traits of each group of black pigs were determined and statistically analyzed, and the results are shown in the following table: ; From the above table, the results of this feeding experiment fully verify the effect of the synergistic use of the fat directional deposition complex enzyme group, the amino acid-enzyme synthesis substrate combination and the lipase specific inhibitor.

[0055] In terms of core target directional improvement of intramuscular fat (IMF), Example One shows absolute advantages. The IMF content of its longissimus dorsi muscle reaches 4.52%, which is significantly higher than all example groups (P<0.05). Compared with the use of enzyme group alone (Example Two, 3.41%) or amino acid combination (Example Three, 2.97%), Example One proves that the "enzyme-substrate" precise matching can significantly improve the synthesis efficiency. Moreover, even if the enzyme group and the amino acid combination are used at the same time (Example Five, 3.89%), the IMF content is still significantly lower than Example One, which clearly shows that the lack of blocking of the decomposition pathway by the lipase specific inhibitor leads to significant loss of intramuscular fat that has been synthesized, and the net deposition efficiency is limited. The result of Example Eight using ordinary complex enzyme (3.05%) further confirms that non-directional digestive enzymes cannot replace the special enzyme group targeting the fat synthesis pathway in the present application.

[0056] In terms of balancing the improvement of IMF and body fat, Example One achieves the best results. While achieving the highest IMF, Example One has the lowest back fat thickness (22.5mm), which is not significantly different from Example Five (23.2mm), but significantly lower than Example Six (25.7mm) using only enzyme group and inhibitor, and Example Seven (25.2mm) lacking the core enzyme group. This shows that the complete system of the present application can direct the nutrient flow and metabolic reaction to intramuscular deposition, effectively avoiding the "overflow" of energy to subcutaneous fat. At the same time, the eye muscle area of Example One is the largest (48.7cm²), indicating that this scheme optimizes meat quality while fully ensuring the improvement of muscle growth and lean meat rate.

[0057] In terms of growth performance, Example One shows the unity of efficiency and economy. Its feed conversion ratio (3.08) is the lowest among all groups, which is equivalent to Example Five and Eight, but significantly better than other examples lacking any core component, which shows that the complete synergistic system optimizes the use of nutrients, directing more ingested energy to be converted into high-value intramuscular fat and muscle protein, rather than being used for maintenance or converted into useless body fat, thereby improving feed reward.

[0058] In summary, the results of Example 1 and the significant differences between each example demonstrate that there is an inseparable synergistic effect between the fat directional deposition complex enzyme group, the amino acid-enzymatic synthesis substrate combination and the lipase specific inhibitor, which together constitute a regulation system from promoting directional synthesis, providing exclusive substrates to inhibiting ineffective decomposition. This system is used to solve the problem of low efficiency, poor directionality and antagonism with lean meat growth of intramuscular fat deposition in black pig production, and realizes the simultaneous optimization of quality and efficiency in black pig production.

[0059] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with a preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the above disclosed technical content to obtain equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any modification, change and modification of the above embodiments according to the technical essence of the present application are still within the scope of the technical solution of the present application.

Claims

1. A black pig intramuscular fat regulating nutritional feed containing a complex enzyme preparation, characterized in that, The black pig intramuscular fat regulation nutrition feed is composed of 850-920 parts by weight of base feed, 5-12 parts by weight of fat directional deposition complex enzyme group, 25-40 parts by weight of amino acid-enzymatic synthesis substrate combination and 2-6 parts by weight of lipase specific inhibitor. The fat directional deposition complex enzyme group is compounded by lipoprotein lipase, acetyl-CoA carboxylase and fatty acid synthase at a mass ratio of (2-3):(4-5):(2-4). The amino acid-enzymatic synthesis substrate combination is compounded by leucine, isoleucine, glutamic acid and alanine at a mass ratio of (3-5):(2-3):(1-2):(1-2). The lipase specific inhibitor is a natural tea polyphenol modified product.

2. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, The base feed is composed of 500-580 parts by weight of corn flour, 180-220 parts by weight of soybean meal, 60-80 parts by weight of bran, 40-60 parts by weight of secondary powder, 8-12 parts by weight of stone powder, 5-8 parts by weight of calcium hydrogen phosphate and 3-5 parts by weight of salt.

3. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, In the fat directional deposition complex enzyme group, the enzyme activity of lipoprotein lipase is greater than or equal to 2000 U / g, the enzyme activity of acetyl-CoA carboxylase is greater than or equal to 1500 U / g, and the enzyme activity of fatty acid synthase is greater than or equal to 1800 U / g.

4. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, The leucine, isoleucine, glutamic acid and alanine in the amino acid-enzymatic synthesis substrate combination are all food-grade L-type amino acids, and the purity of each amino acid is greater than or equal to 99%.

5. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, The preferred mass ratio of the fat directional deposition complex enzyme group is lipoprotein lipase:acetyl-CoA carboxylase:fatty acid synthase=2:5:

3. The preferred mass ratio of the amino acid-enzymatic synthesis substrate combination is leucine:isoleucine:glutamic acid:alanine=4:2:1:

1.

6. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, The feed product is in the form of granules, the water content is controlled to be 10-12%, the granule hardness is 2.5-3.0 kg / cm2, and the granule particle size is 3-4 mm.

7. The black pig intramuscular fat regulation feed containing the complex enzyme preparation according to claim 1, characterized in that, The preparation process of the natural tea polyphenol modified product is as follows: The tea polyphenol and citric acid are mixed at a mass ratio of 5:1, then placed in a 60-65°C water bath environment for constant temperature stirring at 200 r / min for 2 h, cooled to room temperature, dried to a water content of less than or equal to 5%, and pulverized to 100 mesh to obtain the product.

8. A method for preparing a black pig intramuscular fat regulation nutrition feed containing a complex enzyme preparation, which is suitable for the black pig intramuscular fat regulation nutrition feed containing a complex enzyme preparation according to any one of claims 1-6, characterized in that, The steps of the method are as follows: S100, the corn flour, soybean meal, bran and secondary powder in the base feed are respectively pulverized to 80-100 mesh and sieved, and then uniformly mixed to obtain a base mixture; the stone powder, calcium hydrogen phosphate and salt are ground to 120 mesh and sieved, and then uniformly mixed to obtain a mineral premix, which is ready for use; S200, the lipoprotein lipase, acetyl-CoA carboxylase and fatty acid synthase are weighed, stirred at 150-200 r / min at room temperature for 10-15 min, and then uniformly mixed to obtain a fat directional deposition complex enzyme group, which is sealed and stored away from light; S300, the leucine, isoleucine, glutamic acid and alanine are weighed, stirred at 120-150 r / min at room temperature for 8-10 min, and then uniformly mixed to obtain an amino acid-enzymatic synthesis substrate combination, which is ready for use for preparing a lipase specific inhibitor. S400, the base mixture of S100 and the mineral premix are put into a horizontal mixer, and stirred at 250-300 r / min for 20-25 min to obtain a base material, then the fat directional deposition complex enzyme group and the amino acid-enzyme synthesis substrate combination are added, and stirred at 200-250 r / min for 15-20 min, then the lipase specific inhibitor is added, and stirred at 180-200 r / min for 10-12 min, until there is no obvious granular material, to obtain a mixture; S500, the mixture is sent into a granulator, the granulation temperature is controlled at 70-75 ℃, the ring die aperture is 3-4 mm, and granular feed is prepared, which is cooled to room temperature by a cooler, and then screened to remove broken materials, to obtain finished feed.

9. The method for preparing a black pig intramuscular fat-regulating nutritional feed containing a compound enzyme preparation according to claim 8, characterized in that, In the S200, when the fat directional deposition complex enzyme group is prepared, the stirring environment is a light-proof and dry environment, and the environmental humidity is ≤45%, and the storage temperature of the prepared complex enzyme group under sealed light-proof conditions is 2-8 ℃.

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