Soothing and repairing composition containing high-purity oxyresveratrol and application thereof

Abstract

The invention relates to a soothing and repairing composition containing high-purity oxyresveratrol and application of the soothing and repairing composition. The composition is prepared from the following components: high-purity oxyresveratrol, a ganoderma lucidum extract, a hop extract, a cynara scolymus leaf extract and a vegetarian flower extract, the weight ratio of the high-purity oxidized resveratrol to the ganoderma lucidum extract to the humulus lupulus extract to the Huangfang flower extract is (0.05-0.5): (0.1-1): (0.1-0.8): (0.1-1). Through reasonable compounding of the four components of the high-purity oxidized resveratrol, the ganoderma lucidum extract, the humulus lupulus extract and the parsley flower extract, the soothing and repairing effects are achieved in the aspects of promoting collagen regeneration, promoting barrier protein, activating bitter receptors, inhibiting inflammatory factors and the like.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a soothing and repairing composition containing high-purity oxidized resveratrol and its application. Background Technology

[0002] Oxidized resveratrol is a natural derivative of resveratrol, found in plants such as mulberry. Studies have shown that it possesses superior antioxidant and anti-inflammatory activity compared to resveratrol, thus demonstrating great potential in soothing, repairing, and anti-aging.

[0003] Most commercially available oxidized resveratrol is obtained through plant extraction or chemical synthesis, with a purity generally below 98%, containing impurities such as unreacted raw materials and residual solvents. These impurities may not only reduce its efficacy but also cause safety issues such as skin irritation and allergies. Furthermore, due to the presence of these impurities, commercially available oxidized resveratrol typically appears yellow to brownish-yellow. This darker color limits its application in colorless or light-colored skincare products. To maintain the original color of light-colored skincare products, its dosage needs to be reduced, which also leads to a decrease in efficacy. Therefore, purifying existing oxidized resveratrol to improve its purity and refine the color of products using it is of great significance. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a soothing and repairing composition containing high-purity oxidized resveratrol and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a soothing and repairing composition containing high-purity oxidized resveratrol, comprising the following components: high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, artichoke leaf extract, and senna flower extract, wherein the weight ratio of the high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, and senna flower extract is (0.05-0.5):(0.1-1):(0.1-0.8):(0.1-1); The method for preparing high-purity oxidized resveratrol includes the following steps: S1. Heat the oxidized resveratrol powder with a purity of 80-95% to 210-215℃ until the powder is completely melted into a liquid state to obtain the first melt liquid; S2. Cool the crude melt obtained in step S1 at a rate of 2-5℃ / h to 185-190℃, and keep it at the temperature for 1-3h to crystallize the oxidized resveratrol and obtain the first crystal of oxidized resveratrol. The uncrystallized liquid is recovered. S3. Heating and sweating: The oxidized resveratrol crystals obtained in step S2 are heated to 195-199℃ at a rate of 2-3℃ / h and held at the temperature for 0.5-1.5h. The sweating liquid precipitated from the oxidized resveratrol crystals is discharged and recovered to obtain the second oxidized resveratrol crystals. S4. The second crystal of oxidized resveratrol obtained in step S3 is further heated to 210-215℃ to a molten state to obtain a second melt. S5. Repeat steps S2-S4 at least once, collect the second melt, cool and crystallize to obtain the high-purity oxidized resveratrol, wherein the purity of the high-purity oxidized resveratrol is >99.5%.

[0006] Oxidized resveratrol is extracted from mulberry roots. During the extraction and purification process, some components with structures similar to oxidized resveratrol (such as morin A) and some pigments and other impurities may be present. This invention uses a melt crystallization method to prepare high-purity oxidized resveratrol. The melt crystallization method is based on the different crystallization points of the substances being separated, and the separation of the target components is achieved by controlling the temperature, as detailed below: In step S1, oxidized resveratrol powder with a purity of 80-95% is first heated to 210-215℃ to melt, thus obtaining the first melt liquid; Step S2 involves cooling the first molten liquid to 185-190℃ (at a rate of 2-5℃ / h) and holding it at that temperature for 1-3 hours. This temperature range is 10-15℃ below the melting point, which is the supercooled liquid region of oxidized resveratrol. Slow cooling (2-5℃ / h) can avoid crystallization that would cause impurities to be trapped in the crystals due to rapid crystallization. Then, holding the temperature at that time allows the oxidized resveratrol molecules to preferentially form regular crystals, while impurities (such as unoxidized resveratrol, oligomers, residual solvents, etc.) are difficult to enter the crystal lattice due to the difference in melting point between the impurities and the target product (usually the melting point of impurities is lower), and remain in the uncrystallized liquid, thus achieving the initial purification of solid-liquid separation.

[0007] Step S3, the heating and sweating process, is a crucial purification step in the melt crystallization process: small amounts of impurities easily adsorb onto the crystal surface and at the grain boundaries. These impurities typically have melting points slightly lower than those of oxidized resveratrol. Slowly heating to 195-199℃ (close to the target substance's melting point but not completely melting) allows the impurities on the crystal surface to liquefy ("sweat") and be expelled, further purifying the crystal. The heating rate (2-3℃ / h) is lower than the cooling rate to avoid localized overheating that could cause the entire crystal to melt, ensuring that only impurities are removed.

[0008] Step S4 has the same melting temperature as S1, which allows the pure crystals after sweating to reliquefy, providing a uniform liquid raw material for the next round of crystallization, avoiding localized uneven concentration caused by crystal residue, and ensuring consistency across multiple rounds of crystallization.

[0009] The high-purity oxidized resveratrol obtained through the above process has a purity of over 99%. When this high-purity oxidized resveratrol is added to the composition and combined with Ganoderma lucidum extract, hops extract, and senna flower extract, it can further enhance the soothing and repairing effects of the composition. The functions of the above components are as follows: Oxidized resveratrol, a derivative of resveratrol, possesses enhanced antioxidant capacity. It can reduce AGEs induced by glycation end products (AGEs), increase collagen expression, and effectively inhibit MMP-1 expression, thereby reducing collagen degradation. Furthermore, oxidized resveratrol can inhibit the secretion of inflammatory factors such as TNF-α, IL-1β, and IL-6, exhibiting a certain soothing effect. The efficacy of oxidized resveratrol is related to its purity; high purity ensures a higher concentration of active ingredients, enabling more effective inhibition of inflammatory signaling pathways and reducing inflammation at its source. It can also more effectively reduce MMP-1 production and more effectively inhibit collagen degradation.

[0010] Ganoderma lucidum extract is a traditional soothing ingredient. Its polyphenols, polysaccharides, and triterpenoids can inhibit sensory receptors TRPV1 and TRPA1, relieving discomfort. It can also reduce inflammatory factors such as IL-1α and IL-8, achieving a soothing effect. Furthermore, Ganoderma lucidum extract can increase the synthesis of filaggrin, improving the skin barrier.

[0011] Hops extract can activate T2Rs, the sensory bitter taste receptors in the skin, thus enhancing the skin's protective barrier. Furthermore, hops extract can activate the expression of the antimicrobial protein β-defensin-3, resisting bacteria such as Staphylococcus aureus, and exhibiting anti-inflammatory and barrier-strengthening effects.

[0012] The extract of Su Fang flower is rich in essential oils, flavonoids, tannins and other components, and has a calming and astringent effect.

[0013] Preferably, the weight ratio of the high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract and Susania serratifolia flower extract is (0.08-0.2):(0.5-0.9):(0.1-0.3):(0.1-0.5).

[0014] Preferably, the weight ratio of the high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract and Susania serratifolia flower extract is 0.1:0.8:0.2:0.2.

[0015] In a second aspect, the present invention provides a method for preparing the soothing and repairing composition containing high-purity oxidized resveratrol as described in the first aspect, comprising the following steps: T1. Mix the high-purity oxidized resveratrol of the formula amount with 18-23 times the amount of butylene glycol at 20-60℃ to obtain a high-purity oxidized resveratrol solution. T2. Mix the hops extract and the succulent flower extract, then add 8-13 times the amount of water and mix well to obtain a mixed solution. T3. Mix the high-purity oxidized resveratrol solution, the mixed solution, and the Ganoderma lucidum extract to obtain the soothing and repairing composition containing high-purity oxidized resveratrol.

[0016] Thirdly, the present invention provides the application of the soothing and repairing composition containing high-purity oxidized resveratrol in the first aspect in the preparation of cosmetics.

[0017] Preferably, the cosmetic is a toner, lotion, cream, mask, or gel, and the amount of the soothing and repairing composition added is 5%-10% of the total weight of the cosmetic.

[0018] Fourthly, the present invention provides a soothing and repairing lotion comprising the following ingredients by weight percentage: 5%-10% of the soothing and repairing composition containing high-purity oxidized resveratrol as described in the first aspect, 3%-8% of moisturizer, 0.1%-0.3% of thickener, 0.03%-0.1% of chelating agent, 5%-8% of emulsifying agent, 0.5%-2% of preservative, 0.01%-0.3% of pH adjuster, and the balance being deionized water.

[0019] Preferably, the moisturizer is at least one selected from allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerol, budding stalk polysaccharide, and ceramide.

[0020] Preferably, the thickener is at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum.

[0021] Preferably, the chelating agent is EDTA-2Na.

[0022] Preferably, the emulsifying component is at least one of the following: caprylic / capric triglyceride, C14-22 alkyl alcohol / C12-20 alkyl glucoside, coconut oil-caprylate / capric acid ester, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglycerol-6 distearate, and polymethylsilsesquioxane.

[0023] Preferably, the preservative is at least one selected from butanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0024] Preferably, the pH adjuster is arginine and / or tromethamine.

[0025] Fifthly, the present invention provides a method for preparing the soothing and repairing lotion of the fourth aspect, comprising the following steps: (1) Take thickener, humectant and chelating agent, mix them evenly, add deionized water, homogenize at 70-80℃, keep warm for later use, and obtain pre-prepared component A; (2) Mix the emulsified components, homogenize them at 70-80℃, keep them warm for later use, and obtain the pre-prepared component B; (3) Mix the pre-prepared component A and pre-prepared component B, homogenize them, and obtain the emulsified base material; (4) Cool the emulsified base material to 45-55℃, add the soothing and repairing composition, stir until the material is uniform, then add the preservative and pH adjuster, stop stirring, discharge the material, and obtain the soothing and repairing emulsion.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) In this invention, the rational combination of four components—high-purity oxidized resveratrol, Ganoderma lucidum extract, hop extract, and Sufanghua flower extract—achieves a soothing and repairing effect by promoting collagen regeneration, promoting barrier proteins, activating bitter taste receptors, and inhibiting inflammatory factors.

[0027] (2) The high-purity oxidized resveratrol of the present invention is obtained by purifying crude oxidized resveratrol (purity of 80-94%) by melt crystallization. The purified oxidized resveratrol has a lighter and more transparent color when applied to the product, which broadens the application of the composition in colorless or light-colored skin care products. Attached Figure Description

[0028] Figure 1 The images show the physical samples of the high-purity oxidized resveratrol ethanol solutions from Example 1 and Comparative Example 9. Detailed Implementation

[0029] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The sources of the raw materials used in the following examples and comparative examples are as follows: Commercially available resveratrol oxidized powder: manufactured by Shanghai Keqin Technology Co., Ltd.; Ganoderma lucidum extract: manufactured by Seppic, trade name Ganocalm™; Hops extract: The manufacturer is Xi'an Tianzhicui Biotechnology Co., Ltd., and the product name is hops extract. Sufang Flower Extract: The manufacturer is Xi'an Huaxing Biotechnology Co., Ltd., and the product name is Sufang Flower Extract.

[0031] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0032] Examples 1-5 and Comparative Examples 1-5 Specifically, the method for preparing high-purity oxidized resveratrol includes the following steps: S1. Heating commercially available resveratrol oxidized powder to 212°C until the powder is completely melted into a liquid state, to obtain the first melt liquid; S2. The crude melt obtained in step S1 is cooled at a rate of 4℃ / h to 187℃ and held at that temperature for 2h to allow the oxidized resveratrol to crystallize, resulting in the first crystal of oxidized resveratrol. The uncrystallized liquid is then recovered. S3. Heating and sweating: The oxidized resveratrol crystals obtained in step S2 are heated to 197°C at a rate of 2°C / h and kept at the temperature for 1h. The sweating liquid precipitated from the oxidized resveratrol crystals is discharged and recovered to obtain the second oxidized resveratrol crystals. S4. The second crystal of oxidized resveratrol obtained in step S3 is further heated to 212°C until it melts, to obtain the second melt liquid; S5. Repeat steps S2-S4 once, collect the second melt, cool and crystallize to obtain the high-purity oxidized resveratrol.

[0033] The components and their masses of the soothing and repairing compositions containing high-purity oxidized resveratrol in Examples 1-5 and Comparative Examples 1-5 are shown in Table 1. The total mass of each composition is equal.

[0034] The preparation methods of the soothing and repairing compositions containing high-purity oxidized resveratrol in Examples 1-5 and Comparative Examples 1-5 include the following steps: T1. Mix the high-purity oxidized resveratrol of the formula amount with 20 times the amount of butylene glycol at 45°C to obtain a high-purity oxidized resveratrol solution. T2. Mix the hops extract and the succulent flower extract, then add 10 times the amount of water and mix well to obtain a mixed solution. T3. Mix the high-purity oxidized resveratrol solution, the mixed solution, and the Ganoderma lucidum extract to obtain the soothing and repairing composition containing high-purity oxidized resveratrol.

[0035] Table 1. Components and their mass ratios in the soothing and repairing compositions containing high-purity oxidized resveratrol in each group.

[0036] Comparative Example 5 The only difference between Comparative Example 5 and Example 1 is that in step S2 of the preparation of high-purity oxidized resveratrol, the crude melt obtained in step S1 is cooled at a rate of 1℃ / h to 187℃ and held at that temperature for 2h to allow the oxidized resveratrol to crystallize, thereby obtaining the first crystal of oxidized resveratrol, and the uncrystallized liquid is recovered.

[0037] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that in step S2 of the preparation of high-purity oxidized resveratrol, the crude melt obtained in step S1 is cooled at a rate of 6°C / h to 187°C and held at that temperature for 2 hours to allow oxidized resveratrol to crystallize, thereby obtaining the first crystal of oxidized resveratrol, and the uncrystallized liquid is recovered.

[0038] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that the preparation step S3 of the high-purity oxidized resveratrol is heating and sweating: the oxidized resveratrol crystals obtained in step S2 are heated to 212°C at a rate of 1°C / h and kept at a constant temperature for 1h. The sweating liquid precipitated from the oxidized resveratrol crystals is discharged and recovered to obtain the second oxidized resveratrol crystals.

[0039] Comparative Example 8 The only difference between Comparative Example 8 and Example 1 is that the preparation step S3 of the high-purity oxidized resveratrol is heating and sweating: the oxidized resveratrol crystals obtained in step S2 are heated to 212°C at a rate of 4°C / h and kept at a constant temperature for 1h. The sweating liquid precipitated from the oxidized resveratrol crystals is discharged and recovered to obtain the second oxidized resveratrol crystals.

[0040] Comparative Example 9 The only difference between Comparative Example 9 and Example 1 is that the high-purity resveratrol oxide in the composition is replaced with commercially available resveratrol oxide.

[0041] Test Example 1: Purity determination of oxidized resveratrol before and after purification The purity of oxidized resveratrol was tested by HPLC. The test samples were prepared by dissolving unpurified oxidized resveratrol (Comparative Example 9) and high-purity oxidized resveratrol from Examples 1 and 5-8 in methanol to a concentration of 1 mg / mL. The test conditions were as follows: C18-250 mm × 4.6 mm × 5 µm column, column temperature 30 °C, flow rate 1.0 mL / min, detection wavelength 326 nm, injection volume 2 µL, and mobile phase as shown in Table 2.

[0042] Table 2 Data of the mobile phase Time / min A(water) / % B (acetonitrile) / % 0 78 22 15 76 24 24 5 95 30 5 95 31 78 22 40 78 22 The purity of oxidized resveratrol in the test sample was calculated using the following peak area normalization formula: ; Where: Amain peak—the peak area of ​​the main peak in the sample solution; Atotal peak—the sum of the peak areas in the sample solution. The calculation results are shown in Table 3.

[0043] Table 3 Purity data of oxidized resveratrol in each group sample purity / % Example 1 99.6 Comparative Example 5 98.5 Comparative Example 6 97.1 Comparative Example 7 98.1 Comparative Example 8 97.6 Comparative Example 9 (before purification) 92.2 As shown in Table 3, the purity of resveratrol oxidized in Examples 1, Comparative Examples 5-8 and Comparative Example 9 was improved.

[0044] Based on the data from Example 1 and Comparative Examples 5-6, the purity of the oxidized resveratrol obtained in Comparative Examples 5-6 was lower than that in Example 1. This may be because the cooling rate in step S2 of Comparative Example 5 was too slow, resulting in slow crystal growth and potential crystal agglomeration, which encapsulated a small amount of impurities and reduced purity. In contrast, the cooling rate in step S2 of Comparative Example 6 was too fast, leading to rapid crystal precipitation, irregular crystal structure, and easy encapsulation of uncrystallized impurities, resulting in increased intracrystalline impurities and a significant decrease in purity. This indicates that the cooling rate in step S2 is one of the factors affecting the purity of oxidized resveratrol. A cooling rate of 2-5℃ / h in step S2 of the preparation of high-purity oxidized resveratrol can significantly improve its purity.

[0045] Based on the data from Example 1 and Comparative Examples 7-8, the purity of the oxidized resveratrol obtained in Comparative Examples 7-8 was lower than that in Example 1. This may be because the cooling rate in step S3 of Comparative Example 7 was too slow, resulting in insufficient crystal evaporation and incomplete liquefaction and removal of impurities on the crystal surface or at grain boundaries, thus affecting purity. In contrast, the cooling rate in step S3 of Comparative Example 8 was too fast, leading to excessively rapid local heating. This caused not only impurities on the crystal surface to melt, but also some of the target crystals to melt simultaneously, reducing both yield and purity. This indicates that the cooling rate in step S3 is also a factor affecting the purity of oxidized resveratrol.

[0046] The oxidized resveratrol from Example 1 and Comparative Example 9 was added to anhydrous ethanol to prepare a 1% (w / w) solution. Figure 1 As can be seen, the left side shows the ethanol solution of commercially available oxidized resveratrol (Comparative Example 9), and the right side shows the ethanol solution of high-purity oxidized resveratrol (Example 1). The comparison shows that the ethanol solution of high-purity oxidized resveratrol (Example 1) is lighter in color and clearer, while the ethanol solution of commercially available oxidized resveratrol (Comparative Example 9) is darker in color and more turbid. This indicates that the purity of oxidized resveratrol is a key factor affecting the solution color, and the present invention can significantly improve the purity of oxidized resveratrol by limiting the cooling rate of steps S2 and S3 in the preparation process.

[0047] Test Example 2: Collagen Content Test of the Composition The compositions of Examples 1-5 and Comparative Examples 1-9 were used as test samples to determine their effect on collagen I content. The method is as follows: Cell seeding and recovery culture: Human dermal fibroblasts (HSF) in the logarithmic growth phase were harvested, digested with trypsin to prepare a single-cell suspension, and counted using a cell counting chamber to adjust the cell density to 2000 cells / 100 μL. 100 μL of the above cell suspension (containing 2000 cells) was added to each well of a 96-well cell culture plate. The culture medium used was DMEM complete medium containing 10% fetal bovine serum (FBS) + 1% penicillin / streptomycin. The seeded 96-well plates were placed in a 37°C, 5% CO2 incubator and cultured for 24 h to allow cell adhesion and recovery. After 24 h, the 96-well plates were removed, and the supernatant in each well was carefully aspirated using a pipette. Grouping: Divide the cells into a control group and an experimental group, with 3-5 replicates in each group to ensure experimental reproducibility.

[0048] Blank group: Add 100 μL of pure DMEM medium without FBS and antibiotics to each well; Experimental group: The test samples (combinations of Examples 1-5 and Comparative Examples 1-9) were first dissolved in DMSO to prepare a stock solution, then diluted with DMEM medium containing 10% FBS + 1% penicillin / streptomycin to a final concentration of 0.1%. 100 μL of this drug-containing medium was added to each well. The treated 96-well plates were returned to a 37°C, 5% CO2 cell culture incubator and cultured for another 24 h. Cells from each group were collected, the medium was discarded, and the cells were washed twice with PBS. 1 mL of TRIzol lysis buffer was added, cells were scraped, transferred to EP tubes, and stored at -80°C for later use. The content of type I collagen in HSF cells was detected using a human type I collagen (ColⅠ) ELISA kit (ELISA kit, catalog number ml057630). The results were expressed as protein content promotion rate: Protein content promotion rate = |(protein content... 实验组 - Protein content 空白组 Protein content 空白组 ×100%. The type I collagen promotion rate reflects the composition's ability to promote the synthesis of type I collagen in the skin. A higher promotion rate indicates that the composition can stimulate skin cells to synthesize more type I collagen, thereby enhancing the skin's repair ability and barrier function, achieving a better soothing and repairing effect. Test results are shown in Table 4.

[0049] Table 4. Type I collagen promotion rate data for each composition Group / Performance Type I collagen promotion rate (%) Example 1 231.3 Example 2 218.4 Example 3 223.6 Example 4 209.1 Example 5 202.8 Comparative Example 1 110.7 Comparative Example 2 126.8 Comparative Example 3 131.4 Comparative Example 4 150.2 Comparative Example 5 190.3 Comparative Example 6 167.8 Comparative Example 7 184.5 Comparative Example 8 174.1 Comparative Example 9 145.0 As shown in Table 4, and in conjunction with the data from Examples 1-5, when the weight ratio of high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, and Susania spicata flower extract is within the range of (0.08-0.2):(0.5-0.9):(0.1-0.3):(0.1-0.5), the composition exhibits a better type I collagen promotion rate, meaning the composition has a better soothing and repairing effect.

[0050] Based on the data from Example 1 and Comparative Examples 1-4, it can be seen that when any one of high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, and Susania spicata flower extract is missing, the soothing and repairing effect of the composition is significantly reduced, indicating that the above four components synergistically improve the soothing and repairing effect of the composition.

[0051] As can be seen from the data of Example 1 and Comparative Examples 4-9, the type I collagen promotion rate of the compositions of Comparative Examples 4-9 is lower than that of Example 1. Combined with the purity data of oxidized resveratrol in Table 3, it can be seen that the higher the purity of oxidized resveratrol, the better the soothing and repairing effect of the composition.

[0052] Application Example 1-5 and Comparative Application Example 1-9 The compositions of Examples 1-5 and Comparative Examples 1-9 were added to the emulsion at a concentration of 8 wt% to obtain the soothing and repairing emulsions of Application Examples 1-5 and Comparative Application Examples 1-9. The formulations are shown in Table 5.

[0053] The preparation methods of the soothing and repairing lotions used in Examples 1-7 and Comparative Examples 1-5 include the following steps: (1) Take thickener, humectant and chelating agent, mix them evenly, add deionized water, homogenize at 75°C, keep warm for later use, and obtain pre-prepared component A; (2) Mix the emulsified components, homogenize them at 75°C, keep them warm for later use, and obtain the pre-prepared component B; (3) Mix the pre-prepared component A and pre-prepared component B, homogenize them, and obtain the emulsified base material; (4) Cool the emulsified base material to 50°C, add the soothing and repairing composition, stir until the material is uniform, then add the preservative and pH adjuster, stop stirring, discharge the material, and obtain the soothing and repairing emulsion.

[0054] Table 5. Soothing and Repairing Lotion Formulations for Application Examples 1-5 and Comparative Application Examples 1-9

[0055] Comparative Application Example 10 Compared to Application Example 10, the emulsion did not contain a soothing and repairing composition containing high-purity oxidized resveratrol, but instead used an equal amount of deionized water instead of the composition. The preparation method was the same as in Application Example 1.

[0056] Test Example 3: Human Efficacy Test of Each Group of Lotions The emulsions prepared using Application Examples 1-5 and Comparative Application Examples 1-10 were used as test samples to test their skin soothing and repair effects. The method is as follows: (1) Seventy-five volunteers aged 18-45 (facial TWEL ≥ 20 g / m²h) were selected and randomly divided into 15 groups of 5 people each. During the test, each group randomly used the lotion from Application Examples 1-5 and Control Application Examples 1-10, once in the morning and once in the evening. The method of use was to apply 1 g of the sample evenly to the entire face after cleansing and moisturizing in the morning and evening, and gently massage until fully absorbed. Follow-up visits were conducted on days 0 and 7 to test various indicators.

[0057] (2) On the days of follow-up visits on days 0 and 7, the subjects did not apply any skin care products. After cleansing, they sat quietly for 30 minutes in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10%. The initial TEWL value of the subjects after cleansing was tested using the Tewameter®™ Hex (Courage+Khazaka) transdermal moisture loss probe. The Visia-7 was used to take facial pictures, and the exported red area images were analyzed to obtain the initial a* value. (3) Analyze the improvement of each indicator, and take the average value of the data. The improvement is calculated as follows: Skin redness a* value improvement rate (%) = (a* value) T0 -a* value T7 ) / a*value T0 ×100%; TEWL value improvement rate = (TEWL value) T0 -TEWL value T7 ) / TEWL value T0 ×100%. See Table 6 for specific data.

[0058] Table 6. Results of human efficacy tests for each group of emulsions. Group / Performance a* value improvement rate TEWL value improvement rate Application Example 1 9.8 14.3 Application Example 2 8.9 12.9 Application Example 3 10.2 14.5 Application Example 4 8.3 13.4 Application Example 5 8.1 12.6 Comparative Application Example 1 4.5 6.5 Comparative Application Example 2 4.9 7.4 Comparative Application Example 3 5.2 7.9 Comparative Application Example 4 6.1 9.0 Comparative Application Example 5 7.9 11.7 Comparative Application Example 6 6.8 10.2 Comparative Application Example 7 7.6 11.0 Comparative Application Example 8 7.2 10.5 Comparative Application Example 9 6.1 8.6 Comparative Application Example 10 3.8 5.7 As shown in Table 6, and combining the data from Application Example 1 and Comparative Application Examples 1-4, it can be seen that the four components in this invention—high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, and Susania spicata flower extract—can synergistically achieve a soothing and repairing effect.

[0059] Combining the data from Application Example 1 and Comparative Application Examples 5-9, it can be seen that the higher the purity of oxidized resveratrol, the better the soothing and repairing effect of the emulsion.

[0060] In summary, this invention improves the soothing and repairing effect of the emulsion by rationally combining four components: high-purity oxidized resveratrol, Ganoderma lucidum extract, hops extract, and Susania spicata flower extract, obtained through a specific preparation method.

[0061] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A soothing repair composition containing high purity oxyresveratrol, characterized in that, The composition comprises high-purity oxymatrine, red ganoderma extract, hop extract, chrysanthemum extract and flos lonicerae extract, and the weight ratio of the high-purity oxymatrine, red ganoderma extract, hop extract and flos lonicerae extract is (0.05-0.5):(0.1-1):(0.1-0.8):(0.1-1). The preparation method of the high-purity oxymatrine comprises the following steps: S1, heat the oxymatrine powder with a purity of 80-95% to 210-215°C until the powder is completely melted into a liquid state to obtain a first molten liquid; S2, cool the crude molten liquid obtained in step S1 at a rate of 2-5°C / h to 185-190°C, and keep the temperature constant for 1-3h to make the oxymatrine crystallize, and recover the liquid that does not crystallize to obtain oxymatrine first crystals; S3, temperature rising and sweating: heat the oxymatrine crystals obtained in step S2 to 195-199°C at a rate of 2-3°C / h, keep the temperature constant for 0.5-1.5h, discharge and recover the sweating liquid in which the oxymatrine crystals are precipitated to obtain oxymatrine second crystals; S4, continue to heat the oxymatrine second crystals obtained in step S3 to 210-215°C to a molten state to obtain a second molten liquid; S5, repeat steps S2-S4 at least once, collect the second molten liquid, and cool and crystallize to obtain the high-purity oxymatrine, and the purity of the high-purity oxymatrine is >99.5%.

2. The soothing repair composition containing high purity oxo-7-hydroxy-8- methoxy-tetrahydrodibenzo[a,h]anthracene-5,6-diol of claim 1, wherein The weight ratio of the high-purity oxymatrine, red ganoderma extract, hop extract and flos lonicerae extract is (0.08-0.2):(0.5-0.9):(0.1-0.3):(0.1-0.5).

3. The soothing repair composition containing high purity oxo-7-hydroxy-8, 9- dehydroferulaldehyde of claim 1, wherein, The weight ratio of the high-purity oxymatrine, red ganoderma extract, hop extract and flos lonicerae extract is 0.1:0.8:0.2:0.

2.

4. Process for the preparation of the soothing repair composition containing high purity oxyresveratrol according to any one of claims 1 to 3, characterized in that, The preparation method comprises the following steps: T1, mix the formula amount of high-purity oxymatrine with 18-23 times the amount of butanediol at 20-60°C to obtain a high-purity oxymatrine solution; T2, mix the hop extract and flos lonicerae extract, then add 8-13 times the amount of water and mix to obtain a mixed solution; T3, mix the high-purity oxymatrine solution, the mixed solution and the red ganoderma extract to obtain the soothing and repairing composition containing high-purity oxymatrine.

5. Use of the soothing and repairing composition containing high-purity oxymatrine according to any one of claims 1-3 in the preparation of a cosmetic.

6. Use of the high purity oxo-homosalen-containing soothing repair composition according to claim 5 for the preparation of a cosmetic product, characterized in that, The cosmetic is a cosmetic water, emulsion, cream, mask or jelly, and the addition amount of the soothing and repairing composition is 5%-10% of the total weight of the cosmetic.

7. A soothing repair emulsion characterized in that, The cosmetic comprises the following raw materials in the following weight percentages: 5%-10% of the soothing and repairing composition containing high-purity oxymatrine according to any one of claims 1-3, 3%-8% of a humectant, 0.1%-0.3% of a thickening agent, 0.03%-0.1% of a chelating agent, 5%-8% of an emulsifying component, 0.5%-2% of a preservative, 0.01%-0.3% of a pH regulator, and the balance is deionized water.

8. The soothing repair lotion of claim 7, wherein, The raw materials are selected from at least one of (a)-(f): (a) the humectant is at least one of allantoin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,3-butanediol, glycerin, pullulan, and ceramide; (b) the thickening agent is at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and arabinogalactan; (c) the chelating agent is EDTA-2Na; (d) the emulsifying ingredient is at least one of caprylic / capric triglyceride, C14-22 alkyl alcohol / C12-20 alkyl glucoside, coco-caprylate / caprate, dimethicone, hydrogenated palm kernel oil, cetostearyl alcohol, polyglyceryl-6 diisostearate, and methicone; (e) the preservative is at least one of 1,2-hexanediol and p-hydroxyacetophenone; (f) the pH adjuster is arginine and / or tromethamine.

9. Process for the preparation of a soothing repair emulsion according to claim 8, characterized in that, The steps include: (1) Mix the thickening agent, humectant, and chelating agent, add deionized water, homogenize at 70-80°C, and keep warm for standby, to obtain a pre-prepared A component; (2) Mix the emulsifying ingredients, homogenize at 70-80°C, and keep warm for standby, to obtain a pre-prepared B component; (3) Mix the pre-prepared A component and the pre-prepared B component, homogenize, to obtain an emulsified base; (4) Cool the emulsified base to 45-55°C, add the soothing and repairing composition, stir until the material is uniform, then add the preservative and the pH adjuster, stop stirring, discharge, and obtain the soothing and repairing emulsion.

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