Oil control and hair growth composition and preparation method thereof

Abstract

The invention relates to an oil-controlling and hair-growing composition and a preparation method thereof, and belongs to the technical field of cosmetics. The oil-controlling and hair-growing composition is prepared from the following components in percentage by mass: 0.5 to 1 percent of lotus corniculatus extract, 0.1 to 0.2 percent of biotin, 2 to 3 percent of cacumen biotae extract, 1 to 2 percent of ginger root extract, 1 to 5 percent of lactobacillus fermented folium eriobotryae extracting solution, 30 to 50 percent of butanediol and the balance of deionized water. All the components in the formula are matched and synergistically solve the problems of excessive grease, hair follicle atrophy and insufficient blood supply in seborrheic alopecia, and the synergistic oil control and hair growth effect is achieved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to an oil-controlling and hair-growth composition and its preparation method. Background Technology

[0002] Seborrheic alopecia, also known as androgenetic alopecia, is the most common type of hair loss in clinical practice, accounting for more than 70% of all types of hair loss. Its root cause is closely related to genetics and abnormal androgen metabolism. It is characterized by excessive secretion of scalp oil, miniaturization of hair follicles, and a reduction in the proportion of hair follicles in the growth phase, which ultimately leads to thinning and hair loss.

[0003] Currently, interventions for seborrheic alopecia mainly fall into two categories: chemical drugs and plant extracts, but both have certain limitations. Chemical drugs, represented by minoxidil, are commonly used in clinical practice. For example, CN120661520A discloses a hair growth-promoting series composition for treating hair loss, its preparation method, and its application, which uses minoxidil as the active ingredient for hair growth. Although minoxidil has been proven to promote hair growth, it commonly causes adverse reactions such as scalp itching, contact dermatitis, and accelerated early hair loss. It also exhibits drug dependence, is prone to relapse after discontinuation, and is poorly tolerated by some patients, limiting its long-term and widespread application.

[0004] To avoid the side effects of chemical drugs, research and development have shifted towards natural plant extracts. However, many solutions target only a single pathological aspect, resulting in limited effectiveness. For example, CN108743695A discloses a traditional Chinese medicine composition and preparation for hair growth and protection. The active ingredients are a traditional Chinese medicine composition composed of Sanguisorba officinalis, Taraxacum mongolicum, Ginkgo biloba, etc., prepared using an alcohol extraction method. Although it has a certain therapeutic effect on seborrheic alopecia, it is insufficient in terms of hair growth efficacy and cannot simultaneously address the core issues of excessive oil production and hair follicle atrophy.

[0005] Therefore, there is an urgent need to develop an oil-controlling hair growth composition and its preparation method that can simultaneously solve the three major problems of excessive oil, hair follicle atrophy and insufficient blood supply through the synergistic effect of specific plant components. Summary of the Invention

[0006] The purpose of this invention is to provide an oil-controlling and hair-growth composition and its preparation method, which has the characteristics of preventing and treating hair loss by regulating scalp sebum secretion, promoting hair follicle cell activation, and improving microcirculation.

[0007] The objective of this invention can be achieved through the following technical solutions: An oil-controlling and hair-growth composition, wherein the oil-controlling and hair-growth composition comprises the following components by weight percentage: Peperomia root extract 0.5-1%, Biotin 0.1~0.2%, 2-3% of Platycladus orientalis leaf extract Ginger root extract 1-2%, Lactobacillus fermented loquat leaf extract 1-5%, Butanediol 30-50%, Deionized water balance.

[0008] As a preferred embodiment of the present invention, the oil-controlling and hair-growth composition comprises the following components by mass percentage: Peperomia root extract 0.8%, Biotin 0.15%, 2.5% of Platycladus orientalis leaf extract Ginger root extract 1.5%, Lactobacillus fermented loquat leaf extract 3%, Butanediol 40%, Deionized water balance.

[0009] As a preferred embodiment of the present invention, the extraction method of the Lactobacillus fermented loquat leaf extract is as follows: S3-1: Inoculate the freeze-dried Lactobacillus powder into MRS liquid medium at a mass-to-volume ratio of 1g:(30-50)mL, and incubate at 35-37℃ until the viable count is ≥1×10⁻⁶. 8 CFU / mL was used to obtain Lactobacillus bacterial culture; S3-2: Mix loquat leaves with a sodium bicarbonate solution with a mass concentration of 5%~15% at a mass ratio of 1:(5~15) and soak for 30~60 min. After removing the surface fuzz, wash and dry the loquat leaves. After pulverizing, pass the solution through a 20~60 mesh sieve to obtain loquat leaf powder. S3-3: Mix loquat leaf powder and lactobacillus bacterial solution at a mass-to-volume ratio of 1g:(20-30)mL, and let it ferment at 35-38℃ for 3-7 days to obtain fermentation liquid; S3-4: Sterilize the fermentation broth at 80~90 ℃ for 10~20 min, and obtain the Lactobacillus fermented loquat leaf extract after centrifugation and filtration.

[0010] As a preferred technical solution of the present invention, the freeze-dried lactobacillus powder in S3-1 is a mixture of freeze-dried Lactobacillus plantarum powder and freeze-dried Lactobacillus rhamnosus powder in a mass ratio of 1:(1~2).

[0011] As a preferred embodiment of the present invention, the preparation method of the Paeonia lactiflora root extract is as follows: S5-1: Mix choline salt and hydrogen bond donor in a molar ratio of 1:(1~2), and heat and stir at 60~80 ℃ until a clear and transparent eutectic solvent is formed; S5-2: Mix the Lobelia chinensis root powder with a eutectic solvent at a mass ratio of 1:(15~30), and extract by stirring at 200~400 rpm at 50~70 ℃ for 1~3 h to obtain a crude extract; S5-3: The crude extract is first filtered through a microfiltration membrane, and the resulting filtrate is then separated and enriched through a nanofiltration membrane. The retentate is collected and then freeze-dried at -30~-40 ℃ for 12 h to obtain the Lobelia chinensis extract.

[0012] In a preferred embodiment of the present invention, the choline salt in S5-1 is choline chloride, and the hydrogen bond donor is either glucose or lactic acid.

[0013] As a preferred embodiment of the present invention, the nanofiltration membrane in step S5-3 has a molecular weight cutoff of 200~500 Da, and the nanofiltration process pressure is controlled at 0.5~1.5 MPa and the temperature at 25~40 ℃.

[0014] As a preferred embodiment of the present invention, the Platycladus orientalis leaf extract is a 70% ethanol reflux extract with a total flavonoid content ≥18%.

[0015] As a preferred embodiment of the present invention, the biotin is microencapsulated, and the encapsulating material is hydroxypropyl-β-cyclodextrin.

[0016] A method for preparing an oil-controlling and hair-growth composition, the specific steps of which are as follows: S10-1: Preheat butylene glycol to 45±2℃, then add Lophatherum gracile root extract, Platycladus orientalis leaf extract and Lactobacillus fermented loquat leaf extract in sequence, and stir at 300~500 rpm to obtain phase A; S10-2: Heat deionized water to 40±2℃, add ginger root extract and biotin, and sonicate at 20~40 kHz for 5~10 min with a sonication power density of 50 W / L to obtain phase B; S10-3: Slowly add phase B to phase A under continuous stirring, mix for ≥30 min, and after the temperature drops to 25℃, adjust the pH to 5.0~5.5 with citric acid, and then filter through a 0.22 μm microporous membrane to obtain the oil-controlling and hair-growth composition.

[0017] Loquat leaves are rich in triterpenoids such as ursolic acid and oleanolic acid, which are natural 5α-reductase inhibitors. These compounds can reduce DHT production, which leads to excessive sebum secretion, at its source, and also possess excellent anti-inflammatory properties. Lactobacillus fermentation is not only an extraction process but also a highly efficient biotransformation process. During fermentation, the lactobacilli secrete various enzyme systems, such as cellulase, pectinase, and protease, which break down large molecules like proteins and polysaccharides in loquat leaves into smaller, more easily absorbed active peptides and short-chain fatty acids.

[0018] The newly generated short-chain fatty acids help maintain a healthy, slightly acidic environment for the scalp, inhibiting the growth of harmful microorganisms such as Malassezia lipophila, and assisting in oil control from the perspective of regulating the scalp's microecology. Small molecule active peptides and converted triterpenoids have higher bioavailability, can penetrate the scalp more effectively, inhibit the release of inflammatory factors, reduce micro-inflammation around hair follicles, and create a healthy environment for hair growth.

[0019] Lactobacillus plantarum and Lactobacillus rhamnosus were combined at a mass ratio of 1:(1~2). Lactobacillus plantarum possesses strong enzyme secretion capabilities and environmental adaptability, efficiently disrupting the cell walls of loquat leaves and releasing intracellular active substances. Lactobacillus rhamnosus is a recognized star probiotic strain, with significant effects on immune regulation and anti-allergy activity from itself and its metabolites. The combination of these two strains produces a richer spectrum of enzymes and metabolites, enabling a more comprehensive and efficient transformation of loquat leaves compared to single-strain fermentation. The resulting extract contains a wider variety and higher concentration of active ingredients, and exhibits more stable and comprehensive oil-controlling and anti-inflammatory effects.

[0020] The pretreatment of loquat leaves involves soaking them in a 5%–15% sodium bicarbonate solution, which effectively removes the fuzz on the leaf surface and some potential sensitizing impurities, significantly reducing the irritation of the final extract to the scalp and making the product more suitable for sensitive scalps. Furthermore, the alkaline environment may soften the plant fiber structure, which is more conducive to the subsequent enzymatic hydrolysis by lactobacilli, improving fermentation efficiency and the dissolution rate of active ingredients.

[0021] This invention utilizes a specific eutectic solvent (NADES) extraction and membrane separation enrichment technique to prepare a high-purity, highly active extract of *Spiraea japonica* root. The NADES solvent, composed of choline chloride and glucose or lactic acid, possesses a complex hydrogen-bonded network structure that exhibits extremely high solubility and selectivity for the active components in *Spiraea japonica* root. Compared to traditional ethanol or water extraction, NADES can extract these active substances from plant cells more fully and completely, thus significantly improving the initial extraction efficiency. NADES has good biocompatibility, and the extraction conditions are relatively mild, avoiding the damage to heat-sensitive or chemically unstable active components caused by high temperatures and highly polar solvents, thus maximizing the preservation of their natural conformation and biological activity. Subsequently, nanofiltration membranes are used to separate the crude extract, effectively enriching the flavonoids in *Spiraea japonica* root that exert 5α-reductase inhibitory effects, while a large number of inorganic salts, monosaccharides, and small molecule pigments in the extract, with their small molecular weights, can be effectively removed by passing through the nanofiltration membrane with the solvent.

[0022] Using 70% ethanol as a solvent to extract Platycladus orientalis leaves can efficiently and selectively dissolve and extract the flavonoid components that play a core role in hair growth, while avoiding the dissolution of too many fat-soluble or water-soluble impurities, thereby effectively promoting the proliferation of hair papilla cells and prolonging the hair follicle growth phase.

[0023] Biotin itself is sensitive to environmental factors such as light, heat, and oxygen, and is easily degraded and inactivated during formulation storage. Microencapsulation with hydroxypropyl-β-cyclodextrin forms a robust inclusion complex, ensuring biotin maintains high chemical stability and biological activity. Furthermore, biotin is a water-soluble vitamin, and its direct penetration through the skin's stratum corneum barrier is inefficient. Hydroxypropyl-β-cyclodextrin, as an excellent transdermal absorption enhancer, can carry the encapsulated biotin molecules more effectively into the skin's surface.

[0024] The active ingredients in ginger, such as gingerol and shogaol, are natural vasodilators and local stimulants. Through gentle stimulation of the scalp, they can effectively promote capillary dilation and accelerate local blood circulation. Ginger extract possesses powerful antioxidant and anti-inflammatory properties, inhibiting the production of inflammatory mediators. Combined with the anti-inflammatory effects of loquat leaf extract and arborvitae leaf extract, it works synergistically to reduce micro-inflammatory conditions around hair follicles and protect them from inflammatory factors. Some active ingredients in ginger have a certain transdermal absorption-enhancing effect, which can help active ingredients such as arborvitae leaf extract penetrate the stratum corneum of the skin more effectively and reach the hair follicle target, thereby improving the overall bioavailability of the formula.

[0025] The components in this invention exhibit significant synergistic effects: *Eriocaulon buergerianum* root extract and *Eriocaulon buergerianum* leaf extract synergistically inhibit 5α-reductase activity and regulate scalp microecology, precisely controlling oil and reducing inflammation from the source; *Platycladus orientalis* leaf extract directly promotes dermal papilla cell proliferation and powerfully activates hair follicles, while ginger root extract improves hair follicle microcirculation, delivering sufficient nutrients for hair follicle growth. Together, they form a dual engine for hair growth, activating and nourishing the follicles; microencapsulated biotin serves as a basic nutrient, stably penetrating the hair follicles and strengthening hair root quality. The synergistic effect of these components addresses three core issues: excessive oil production, hair follicle atrophy, and insufficient blood supply, achieving a synergistic and enhanced oil control and hair growth effect.

[0026] The beneficial effects of this invention are: This oil-controlling and hair-growth composition achieves synergistic effects through scientific formulation and a multi-target synergistic mechanism. Specifically, Lactobacillus fermented loquat leaf extract and Paeonia lactiflora root extract work synergistically. The former enhances oil control and anti-inflammation and regulates scalp microecology through short-chain fatty acids and active peptides produced during fermentation, while the latter effectively inhibits 5α-reductase activity by enriching high-purity flavonoids using low-melting-point solvent coupled nanofiltration technology. Together, they precisely regulate excessive sebum secretion at its source. Regarding hair growth, the high flavonoid content in Platycladus orientalis leaf extract directly stimulates dermal papilla cell proliferation and activates hair follicle vitality; while ginger root extract significantly improves nutrient supply to hair follicles by promoting scalp capillary circulation. These two components form a synergistic pathway for activating hair follicles and ensuring nutrient supply. Microencapsulated biotin, protected by hydroxypropyl-β-cyclodextrin, ensures stability and transdermal absorption, continuously strengthening follicle keratin synthesis and promoting hair root health as a key coenzyme. With the assistance of butylene glycol for optimized penetration, the entire system works in tandem with each component to simultaneously overcome three major challenges: excessive oil production, hair follicle atrophy, and insufficient blood supply, achieving a highly efficient synergy between oil control and hair growth. Detailed Implementation

[0027] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0028] In the embodiments and comparative examples of this invention: Lactobacillus plantarum: purchased from Zhengzhou Fangjue Biotechnology Co., Ltd., strain number HZB222098; Lactobacillus rhamnosus: purchased from Zhengzhou Fangjue Biotechnology Co., Ltd., strain number HZB231391.

[0029] Example 1 An oil-controlling and hair-growth composition, wherein the oil-controlling and hair-growth composition comprises the following components by mass percentage: 0.8% of Paeonia lactiflora root extract, 0.15% of biotin, 2.5% of Platycladus orientalis leaf extract, 1.5% of Zingiber officinale root extract, 3% of Lactobacillus fermented loquat leaf extract, 40% of butylene glycol, and the balance of deionized water; The arborvitae leaf extract is a 70% ethanol reflux extract with a total flavonoid content of ≥18%; the biotin is microencapsulated and the encapsulating material is hydroxypropyl-β-cyclodextrin.

[0030] The extraction method of the Lactobacillus fermented loquat leaf extract is as follows: S3-1: Inoculate the freeze-dried Lactobacillus powder into MRS liquid medium at a mass-to-volume ratio of 1g:40mL and incubate at 36℃ until the viable count is ≥1×10⁻⁶. 8 CFU / mL was used to obtain Lactobacillus bacterial suspension; the freeze-dried Lactobacillus bacterial powder was a mixture of freeze-dried Lactobacillus plantarum and freeze-dried Lactobacillus rhamnosus in a mass ratio of 1:1.5. S3-2: Loquat leaves are mixed with a 10% sodium bicarbonate solution at a mass ratio of 1:10 and soaked for 45 minutes. After removing the surface fuzz, the leaves are washed, dried, pulverized, and passed through a 40-mesh sieve to obtain loquat leaf powder. S3-3: Mix loquat leaf powder and lactobacillus bacterial solution at a mass-volume ratio of 1g:25mL, and let it ferment at 37℃ for 5 days to obtain fermentation liquid; S3-4: The fermentation broth was sterilized at 85 °C for 15 min, and after centrifugation and filtration, the Lactobacillus fermented loquat leaf extract was obtained.

[0031] The preparation method of the Paeonia lactiflora root extract is as follows. S5-1: Mix choline chloride and glucose in a molar ratio of 1:1.5 and heat and stir at 70 °C until a clear and transparent eutectic solvent is formed; S5-2: Mix the Lobelia chinensis powder with a eutectic solvent at a mass ratio of 1:25, and extract by stirring at 300 rpm at 60 ℃ for 2 h to obtain a crude extract; S5-3: The crude extract is first filtered through a microfiltration membrane, and the resulting filtrate is then separated and enriched through a nanofiltration membrane. The molecular weight cutoff of the nanofiltration membrane is 400 Da. The pressure of the nanofiltration process is controlled at 1 MPa and the temperature at 35 °C. The retentate is collected and then freeze-dried at -35 °C for 12 h to obtain the Lobelia chinensis extract.

[0032] A method for preparing an oil-controlling and hair-growth composition, the specific steps of which are as follows: S10-1: Preheat butylene glycol to 45 °C, then add Paeonia lactiflora root extract, Platycladus orientalis leaf extract and Lactobacillus fermented loquat leaf extract in sequence, and stir at 400 rpm to obtain phase A; S10-2: Deionized water was heated to 40 °C, ginger root extract and biotin were added, and the mixture was ultrasonically treated at 30 kHz for 8 min with an ultrasonic power density of 50 W / L to obtain phase B. S10-3: Slowly add phase B to phase A under continuous stirring for 40 min. After the temperature drops to 25 ℃, adjust the pH to 5.2 with citric acid, and then filter through a 0.22 μm microporous membrane to obtain the oil-controlling and hair-growth composition.

[0033] Example 2 An oil-controlling and hair-growth composition, wherein the oil-controlling and hair-growth composition comprises the following components by mass percentage: 0.5% of Paeonia lactiflora root extract, 0.1% of biotin, 2% of Platycladus orientalis leaf extract, 1% of Zingiber officinale root extract, 1% of Lactobacillus fermented loquat leaf extract, 30% of butylene glycol, and the balance of deionized water; The arborvitae leaf extract is a 70% ethanol reflux extract with a total flavonoid content of ≥18%; the biotin is microencapsulated and the encapsulating material is hydroxypropyl-β-cyclodextrin.

[0034] The extraction method of the Lactobacillus fermented loquat leaf extract is as follows: S3-1: Inoculate the freeze-dried Lactobacillus powder into MRS liquid medium at a mass-to-volume ratio of 1g:30mL, and incubate at 35℃ until the viable count is ≥1×10⁻⁶. 8 CFU / mL was used to obtain Lactobacillus bacterial suspension; the freeze-dried Lactobacillus bacterial powder was a mixture of freeze-dried Lactobacillus plantarum and freeze-dried Lactobacillus rhamnosus in a mass ratio of 1:1. S3-2: Mix loquat leaves with a 5% sodium bicarbonate solution at a mass ratio of 1:5 and soak for 30 min. After removing the surface fuzz, wash and dry the mixture. After pulverizing, pass it through a 20-mesh sieve to obtain loquat leaf powder. S3-3: Mix loquat leaf powder and lactobacillus bacterial solution at a mass-volume ratio of 1g:20mL, and let it ferment at 35℃ for 3 days to obtain fermentation liquid; S3-4: The fermentation broth was sterilized at 80 °C for 10 min, and after centrifugation and filtration, the Lactobacillus fermented loquat leaf extract was obtained.

[0035] The preparation method of the Paeonia lactiflora root extract is as follows. S5-1: Mix choline chloride and lactic acid in a molar ratio of 1:1, and heat and stir at 60 °C until a clear and transparent eutectic solvent is formed; S5-2: Mix the Lobelia chinensis powder with a eutectic solvent at a mass ratio of 1:15, and extract by stirring at 200 rpm at 50 ℃ for 1 h to obtain a crude extract; S5-3: The crude extract is first filtered through a microfiltration membrane, and the resulting filtrate is then separated and enriched through a nanofiltration membrane. The molecular weight cutoff of the nanofiltration membrane is 200 Da. The pressure of the nanofiltration process is controlled at 0.5 MPa and the temperature at 25 °C. The retentate is collected and then freeze-dried at -30 °C for 12 h to obtain the Lobelia chinensis extract.

[0036] A method for preparing an oil-controlling and hair-growth composition, the specific steps of which are as follows: S10-1: Preheat butylene glycol to 43°C, then add Paeonia lactiflora root extract, Platycladus orientalis leaf extract and Lactobacillus fermented loquat leaf extract in sequence, and stir at 300 rpm to obtain phase A; S10-2: Deionized water was heated to 38 ℃, ginger root extract and biotin were added, and the mixture was ultrasonically treated at 20 kHz for 5 min with an ultrasonic power density of 50 W / L to obtain phase B. S10-3: Slowly add phase B to phase A under continuous stirring for 35 min. After the temperature drops to 25 °C, adjust the pH to 5.0 with citric acid, and then filter through a 0.22 μm microporous membrane to obtain the oil-controlling and hair-growth composition.

[0037] Example 3 An oil-controlling and hair-growth composition, wherein the oil-controlling and hair-growth composition comprises the following components by mass percentage: 1% of Paeonia lactiflora root extract, 0.2% of biotin, 3% of Platycladus orientalis leaf extract, 2% of Zingiber officinale root extract, 5% of Lactobacillus fermented loquat leaf extract, 50% of butylene glycol, and the balance of deionized water; The arborvitae leaf extract is a 70% ethanol reflux extract with a total flavonoid content of ≥18%; the biotin is microencapsulated and the encapsulating material is hydroxypropyl-β-cyclodextrin.

[0038] The extraction method of the Lactobacillus fermented loquat leaf extract is as follows: S3-1: Inoculate the freeze-dried Lactobacillus powder into MRS liquid medium at a mass-to-volume ratio of 1g:50mL, and incubate at 37℃ until the viable count is ≥1×10⁻⁶. 8CFU / mL was used to obtain Lactobacillus bacterial suspension; the freeze-dried Lactobacillus bacterial powder was a mixture of freeze-dried Lactobacillus plantarum and freeze-dried Lactobacillus rhamnosus in a mass ratio of 1:2. S3-2: Loquat leaves are mixed with a 15% sodium bicarbonate solution at a mass ratio of 1:15 and soaked for 60 min. After removing the surface fuzz, the leaves are washed, dried, pulverized, and passed through a 60-mesh sieve to obtain loquat leaf powder. S3-3: Mix loquat leaf powder and lactobacillus bacterial solution at a mass-volume ratio of 1g:30mL, and let it ferment at 38℃ for 7 days to obtain fermentation liquid; S3-4: The fermentation broth was sterilized at 90 °C for 20 min, and after centrifugation and filtration, the Lactobacillus fermented loquat leaf extract was obtained.

[0039] The preparation method of the Paeonia lactiflora root extract is as follows. S5-1: Mix choline chloride and glucose in a molar ratio of 1:2, and heat and stir at 80 °C until a clear and transparent eutectic solvent is formed; S5-2: Mix the Lobelia chinensis powder with a eutectic solvent at a mass ratio of 1:30, and extract by stirring at 70 ℃ and 400 rpm for 3 h to obtain a crude extract; S5-3: The crude extract is first filtered through a microfiltration membrane, and the resulting filtrate is then separated and enriched through a nanofiltration membrane. The molecular weight cutoff of the nanofiltration membrane is 500 Da. The pressure of the nanofiltration process is controlled at 1.5 MPa and the temperature at 40 °C. The retentate is collected and then freeze-dried at -40 °C for 12 h to obtain the Lobelia chinensis extract.

[0040] A method for preparing an oil-controlling and hair-growth composition, the specific steps of which are as follows: S10-1: Preheat butylene glycol to 47°C, then add Paeonia lactiflora root extract, Platycladus orientalis leaf extract and Lactobacillus fermented loquat leaf extract in sequence, and stir at 500 rpm to obtain phase A; S10-2: Deionized water was heated to 42°C, ginger root extract and biotin were added, and the mixture was ultrasonically treated at 40 kHz for 10 min with an ultrasonic power density of 50 W / L to obtain phase B. S10-3: Slowly add phase B to phase A under continuous stirring for 50 min. After the temperature drops to 25 °C, adjust the pH to 5.5 with citric acid, and then filter through a 0.22 μm microporous membrane to obtain the oil-controlling and hair-growth composition.

[0041] Comparative Example 1 Replace the root extract of *Spiraea pubescens* with an equal amount of ordinary root water extract, and follow the same steps as in Example 1.

[0042] Comparative Example 2 Replace the Lactobacillus fermented loquat leaf extract with an equal amount of ordinary loquat leaf water extract, and the remaining steps are the same as in Example 1.

[0043] Comparative Example 3 Fermentation was performed using only Lactobacillus plantarum, with the remaining steps being the same as in Example 1.

[0044] Comparative Example 4 Fermentation was performed using only Lactobacillus rhamnosus, with the remaining steps being the same as in Example 1.

[0045] Comparative Example 5 The microencapsulated biotin was replaced with an equal amount of untreated biotin powder, and the remaining steps were the same as in Example 1.

[0046] 5α-Reductase Inhibition Experiment (1) Preparation of 5α-reductase extract Animal handling: Six healthy male SD rats were fasted for 12 hours and then anesthetized and euthanized. They were quickly transferred to a 4°C dissection table, where the prostate tissue was completely dissected, minced, and accurately weighed.

[0047] 5α-Reductase Extraction: Prostate tissue was mixed with pre-cooled buffer solution at a mass-to-volume ratio of 1:5. The buffer solution contained 0.32 mol / L sucrose, 0.1 mmol / L dithiothreitol, 1 mmol / L EDTA, and 0.2 mol / L PBS, with a pH of 6.5. The tissue homogenate was rapidly prepared in a homogenizer. The homogenate was centrifuged at 13000 r / min for 10 min at 4°C, and the upper lipid layer and precipitate were discarded, collecting the clear supernatant.

[0048] Enzyme extract preservation: Adjust the supernatant to 20 mL with buffer solution, aliquot into pre-cooled EP tubes, and store at -80℃ for later use.

[0049] (2) 5α-Reductase Activity Assay Test samples: Take the above-prepared composition solution and mix it with deionized water at a mass ratio of 1:4 to obtain test samples 1-8 containing 20wt% composition solution.

[0050] Reaction system: 0.5 mL phosphate buffer, 0.2 mL test sample, 200 μL testosterone solution (300 mg / L) and 200 μL NADPH (reduced coenzyme II) solution (0.8 g / L) were added sequentially to a stoppered test tube. Finally, 0.5 mL of the 5α-reductase extract prepared above was added, and the reaction was carried out at 37 °C for 30 min. Control setup: Positive control: 0.05 mg / L finasteride solution to replace the test sample; Enzyme reaction tube: containing only enzyme extract and substrate (without test sample); Blank tube: without enzyme extract (replaced with an equal volume of buffer solution).

[0051] Detection method: After the reaction was completed, 3 mL of dichloromethane was added to stop the reaction and extract testosterone. Then, 0.25 mL of 100 mg / L propylparaben was added as an internal standard, and the mixture was centrifuged at 5000 r / min for 10 min. The upper aqueous phase was discarded, and about 1 mL of the organic phase was transferred, evaporated to dryness, and the residue was dissolved in 1.5 mL of methanol. 10 μL of the solution was then used to determine the residual testosterone content by high-performance liquid chromatography (HPLC). HPLC conditions: C18 reversed-phase column (4.6 × 250 mm, 5 μm); mobile phase: methanol / water (70:30, v / v); flow rate: 1.0 mL / min; detection wavelength: 254 nm; injection volume: 10 μL.

[0052] Calculate the 5α-reductase inhibition rate: Inhibition rate (%) = (C 样品 -C 反应 ) / (C 空白 -C 反应 ) × 100%; where: C is the ratio of the peak area of ​​testosterone to the peak area of ​​the internal standard.

[0053] Group 5α-Reductase Inhibition Rate (%) Example 1 75.6 Example 2 73.1 Example 3 74.8 Comparative Example 1 68.2 Comparative Example 2 56.3 Comparative Example 3 67.7 Comparative Example 4 61.9 Comparative Example 5 69.5 Positive control group 91.7 Hair regrowth effect test Experimental animals: Seven-week-old male C57BL / 6 mice, weighing 18-22 g, were selected.

[0054] Experimental Methods: After acclimatizing for one week, all mice were divided into nine groups: a negative control group, a positive control group, an example group, and a comparative group, with five mice in each group. Mice were lightly anesthetized, and a 2×2 cm area was selected on their backs as the test area. A rosin / paraffin mixture (mass ratio 1:1) was heated and melted, and then applied to the test area at a suitable temperature. After solidification, the mixture was peeled off to induce hair growth in the mice from the resting phase to the anagen phase. Starting the next day, the sample was evenly applied to the hair-removed area in each group. The negative control group received deionized water, the positive control group received an equal volume of minoxidil solution (1.5 wt%), the example group received an equal volume of the composition prepared in the example, and the comparative group received an equal volume of the composition prepared in the comparative example. The application was performed twice daily, 0.1 mL per mouse each time, for 28 consecutive days. On the last day of the sample application, 10 newly grown hairs were randomly taken from the plucked area of ​​each mouse in each group. The length of each hair was measured and recorded under a magnifying glass using a vernier caliper. The furthest distance between the two ends of the hair was recorded as the hair length, and the average value was calculated.

[0055] Group Hair length (mm) Example 1 7.23 Example 2 7.18 Example 3 7.22 Comparative Example 1 6.53 Comparative Example 2 6.29 Comparative Example 3 6.87 Comparative Example 4 6.69 Comparative Example 5 6.48 Positive control group 7.14 negative control group 4.83 The data above show that the 5α-reductase inhibition rate and the length of mouse hair growth in Examples 1-3 of the present invention are significantly higher than those in the respective comparative proportions, indicating that the composition of the present invention has the effect of preventing hair loss and promoting hair growth.

[0056] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention are still within the scope of the present invention.

Claims

1. An oil control hair growth composition characterized in that, The oil-controlling hair-growing composition comprises the following components by mass percentage: Lotus root extract 0.5~1%, Biotin 0.1~0.2%, Biota leaf extract 2~3%, Ginger root extract 1~2%, Lactobacillus fermented loquat leaf extract 1~5%, Butanediol 30~50%, Deionized water the balance.

2. The oil control hair growth composition according to claim 1, wherein The oil-controlling hair-growing composition comprises the following components by mass percentage: Lotus root extract 0.8%, Biotin 0.15%, Biota leaf extract 2.5%, Ginger root extract 1.5%, Lactobacillus fermented loquat leaf extract 3%, Butanediol 40%, Deionized water the balance.

3. The oil control hair growth composition according to claim 1 or 2, wherein The extraction method of the lactobacillus fermented loquat leaf extract is as follows, S3-1: inoculate the freeze-dried lactobacillus powder into MRS liquid medium at a mass-volume ratio of 1 g: (30-50) mL, and incubate at 35-37 ℃ until the viable bacterial count is ≥1×10 8 CFU / mL, to obtain a lactobacillus bacterial solution; S3-2: Mix loquat leaves with a sodium bicarbonate solution with a mass concentration of 5%~15% at a mass ratio of 1:(5~15)for 30~60 min, wash and dry after removing the surface fluff, crush and pass through a 20~60 mesh sieve to obtain loquat leaf powder; S3-3: Mix the loquat leaf powder with lactobacillus liquid at a mass-volume ratio of 1g:(20~30)mL, and ferment at 35~38℃ for 3~7 days to obtain a fermentation liquid; S3-4: Sterilize the fermentation liquid at 80~90 ℃ for 10~20 min, centrifuge and filter to obtain the lactobacillus fermented loquat leaf extract.

4. An oil control hair growth composition according to claim 3, wherein The lactobacillus freeze-dried bacteria powder in S3-1 is a mixture of Lactobacillus plantarum freeze-dried bacteria powder and Lactobacillus rhamnosus freeze-dried bacteria powder at a mass ratio of 1:(1~2).

5. The oil control hair growth composition according to claim 1 or 2, wherein The preparation method of the lotus root extract is as follows, S5-1: Mix choline salt and hydrogen bond donor at a molar ratio of 1:(1~2), heat and stir at 60~80 ℃ until a clear transparent eutectic solvent is formed; S5-2: Mix lotus root powder with eutectic solvent at a mass ratio of 1:(15~30), stir at a speed of 200~400 rpm at 50~70 ℃ for 1~3 h to obtain a crude extract; S5-3: Filter the crude extract through a microfiltration membrane, separate and enrich the obtained filtrate through a nanofiltration membrane, collect the retentate, and freeze-dry at -30~-40 ℃ for 12 h to obtain the lotus root extract.

6. An oil control hair growth composition according to claim 5, wherein The choline salt in S5-1 is choline chloride, and the hydrogen bond donor is one of glucose and lactic acid.

7. An oil control hair growth composition as claimed in claim 5, wherein The nanofiltration membrane in S5-3 has a molecular weight cut-off of 200~500 Da, and the control nanofiltration process pressure is 0.5~1.5 MPa and the temperature is 25~40 ℃.

8. The oil control hair growth composition according to claim 1 or 2, wherein The biota leaf extract is a 70% ethanol reflux extract, and the total flavonoid content is ≥18%.

9. The oil control hair growth composition according to claim 1 or 2, wherein The biotin is treated by microencapsulation, and the encapsulating material is hydroxypropyl-β-cyclodextrin.

10. A method of preparing the oil-control hair growth composition according to any one of claims 1 to 9, characterized by, The specific steps of the preparation method are as follows, S10-1: Preheat butanediol to 45±2℃, and add lotus root extract, biota leaf extract, and lactobacillus fermented loquat leaf extract in sequence, and stir at a speed of 300~500 rpm to obtain phase liquid A; S10-2: heating deionized water to 40±2℃, adding ginger root extract and biotin in sequence, and treating by ultrasonic at 20~40 kHz for 5~10 min, the power density of ultrasonic treatment being 50 W / L, to obtain phase liquid B; S10-3: slowly adding phase liquid B into phase liquid A under continuous stirring, the mixing time being ≥30 min, adjusting pH to 5.0~5.5 by citric acid after the temperature is reduced to 25℃, and filtering through a 0.22 μm microporous membrane to obtain the oil-controlling hair growth composition.