Herbal composition and preparation for optimizing cell viability and resisting aging

Abstract

The invention provides a herbal medicine composition and a preparation for optimizing cell viability and resisting aging. The herbal medicine composition comprises the following components in percentage by weight: 5%-80% of a rhizoma polygonati extract, 5%-80% of a radix asparagi extract, 5%-80% of a pleurospermum tibetanum extract, 5%-80% of a mirabilis himalaica extract and 5%-80% of a tribulus terrestris extract. The herbal composition has a compound that protects cells from oxidative damage, enhances the cell repair mechanism, and improves the metabolic process with age decline; the composition is beneficial to optimization of cell activity and anti-aging, has wide benefits in anti-inflammatory, anti-oxidation, adaptability and other effects, is used for supporting overall activity, relieving pressure and enhancing immune function, has relatively low side effect risk, and supports anti-aging in a more comprehensive and more balanced manner.

Description

TECHNICAL FIELD

[0001] The present invention relates to pharmaceutical and nutraceutical compositions, and more particularly to a herbal composition, formulation for optimizing cellular vitality and anti-aging. BACKGROUND

[0002] Aging is a multifaceted biological process that includes not only physical changes to one or more bodies, but also complex changes at the cellular and molecular levels. These changes manifest as a gradual decline in the functionality and regenerative capacity of cells, tissues, and organs, leading to decreased physiological capacity and increased susceptibility to various age-related diseases such as cardiovascular disease, diabetes, cognitive decline, and osteoporosis. One of the primary contributors to aging is oxidative stress, which is caused by the accumulation of free radicals that damage cells, proteins, and deoxyribonucleic acid (DNA), thereby accelerating the aging process. Environmental factors, such as pollution, ultraviolet (UV) radiation, poor diet, and chronic stress, further exacerbate the aging process, leading to the early onset of age-related diseases. The cumulative effects of these environmental factors result in a decrease in quality of life, particularly as one or more bodies age.

[0003] The World Health Organization (WHO) emphasizes that the proportion of the global population that is aging is increasing, noting that the percentage of one or more bodies that are aged is projected to grow in the coming years. This shift in population structure has led to increased focus on improving the quality of life for older one or more bodies, with an emphasis on the need for effective interventions to promote healthy aging. While synthetic drugs and supplements are frequently used in current anti-aging practices, these synthetic drugs and supplements come with adverse side effects and limited long-term efficacy. Synthetic drugs and supplements often only target surface symptoms, such as wrinkles and fatigue, without addressing the underlying biological mechanisms of aging. Furthermore, synthetic drugs and supplements can fail to effectively support cellular health and longevity at a deeper level.

[0004] While synthetic longevity supplements can be effective to some extent, they come with drawbacks such as high cost, potential interactions with other drugs, and long-term health risks. Additionally, synthetic supplements fail to address the interconnected nature of aging, which involves not only the physical aspects of the body but also the cellular and molecular health of the body.

[0005] Accordingly, there is a growing need for natural compositions that can effectively support longevity and cellular health while minimizing adverse effects and promoting overall health. SUMMARY

[0006] This summary is intended to introduce selected concepts in a simplified form to provide a basic understanding of the concepts further described in the disclosure. This summary is not intended to identify key or essential inventive concepts or to delineate the scope of the disclosure.

[0007] In order to overcome the defects of the existing synthetic drugs and supplements in side effects and poor effect in optimizing cell vitality and anti-aging, the present application provides a herbal composition and preparation for optimizing cell vitality and anti-aging. The herbal composition has compounds for protecting cells from oxidative damage, enhancing cell repair mechanisms and improving metabolic processes that decline with age; it is beneficial for optimizing cell vitality and anti-aging, and has wide benefits in anti-inflammatory, antioxidant and adaptive effects, for supporting overall vitality, reducing stress and enhancing immune function, with relatively low risk of side effects, to support anti-aging in a more comprehensive and balanced way.

[0008] The present application solves the above technical problems by the following technical solutions:

[0009] The present application provides a herbal composition for optimizing cell vitality and anti-aging, comprising: 5%-80% of an extract of Polygonatum cirrhifolium, 5%-80% of an extract of Asparagus cochinchinensis, 5%-80% of an extract of Pleurospermum hookeri var. thomsonii, 5%-80% of an extract of Oxyba phus himalaicus, and 5%-80% of an extract of Tribulus terrestris; wherein % refers to the mass percentage of each extract in the herbal composition.

[0010] In some embodiments, the herbal composition comprises: 25%-40% of an extract of Polygonatum cirrhifolium, 25%-40% of an extract of Asparagus cochinchinensis, 10%-20% of an extract of Pleurospermum hookeri var. thomsonii, 10%-20% of an extract of Oxyba phus himalaicus, and 10%-15% of an extract of Tribulus terrestris.

[0011] In specific embodiments, the herbal composition comprises: 30%-40% of an extract of Polygonatum cirrhifolium, 30%-40% of an extract of Asparagus cochinchinensis, 10%-15% of an extract of Pleurospermum hookeri var. thomsonii, 10%-15% of an extract of Oxyba phus himalaicus, and 10%-12% of an extract of Tribulus terrestris.

[0012] In a certain embodiment, the herbal composition comprises: 30% of an extract of Polygonatum cirrhifolium, 30% of an extract of Asparagus cochinchinensis, 15% of an extract of Pleurospermum hookeri var. thomsonii, 15% of an extract of Oxyba phus himalaicus, and 10% of an extract of Tribulus terrestris.

[0013] In some embodiments, the preparation method of the herbal composition comprises the following steps in sequence: extracting raw materials of each extract, and then concentrating, separating, encapsulating and mixing each extract; the raw materials comprise Polygonatum sibiricum, Polygonatum sibiricum, Ostericum tsugetense, Mirabilis himalaica and Tribulus terrestris.

[0014] In specific embodiments, the extraction process comprises the following steps in sequence: harvesting the raw materials, and then cleaning, drying, grinding and extracting each raw material to obtain each extract.

[0015] In the present application, the harvesting process is performed at the optimal maturity of each raw material to ensure the optimal concentration of bioactive compounds, which is beneficial to increasing the content of active substances in the subsequent extracts; the optimal maturity is conventional in the art, and the optimal maturity of Polygonatum sibiricum and Polygonatum sibiricum is Polygonatum sibiricum and Polygonatum sibiricum in late autumn of the third growth year, the optimal maturity of Ostericum tsugetense and Mirabilis himalaica is Ostericum tsugetense and Mirabilis himalaica at the flowering stage, and the optimal maturity of Tribulus terrestris is Tribulus terrestris when the fruit is fully developed.

[0016] In preferred embodiments, the cleaning step of each raw material comprises the following steps in sequence: preliminary washing, secondary washing and final rinsing.

[0017] Preferably, the reagent for the preliminary washing comprises purified water containing 0.5% sodium bicarbonate.

[0018] Preferably, the secondary washing is performed by ultrasonic cleaning, and the ultrasonic frequency and power are 40 kHz and 300 W, respectively.

[0019] Preferably, the reagent for the final rinsing is distilled water.

[0020] In the present application, it is known to those skilled in the art that the drying step is performed on the raw material after the cleaning step.

[0021] In the present application, the drying step can be air drying, solar drying, oven drying, freeze drying, microwave drying or infrared drying.

[0022] In preferred embodiments, the drying step comprises the following steps in sequence: cool drying and hot air drying, to retain more active substances in each raw material, thereby improving the stability of the quality of the subsequent preparation.

[0023] Preferably, the temperature and time of the cool drying are 25-30°C.

[0024] Preferably, the temperature and time of the hot air drying are 45°C and 8-12 h, respectively.

[0025] In a preferred embodiment, the dried end point of the Rhizoma Polygonati has a moisture content of 8-12%.

[0026] In a preferred embodiment, the dried end point of the Asparagi has a moisture content of 8-12%.

[0027] In a preferred embodiment, the dried end point of the Orostachys fimbriatus has a moisture content of 6-10%.

[0028] In a preferred embodiment, the dried end point of the Mirabilis himalaica has a moisture content of 5-8%.

[0029] In a preferred embodiment, the dried end point of the Tribulus terrestris has a moisture content of 5-8%.

[0030] In the present application, it is understood by those skilled in the art that the processing object of the grinding step is the raw material processed by the above drying step.

[0031] In the present application, the grinding step is performed in a hammer mill or a cryogenic mill to maintain the integrity of the ingredients in the herbal composition and minimize degradation caused by heat during the grinding process.

[0032] In the present application, each of the raw materials can be ground into a powder with a particle size of 0.1-60 microns; by the grinding step, it is helpful for the active ingredients in each raw material to be leached out in the extraction step to increase the release of active ingredients.

[0033] In a preferred embodiment, the Rhizoma Polygonati and the Asparagi are ground into a powder with a particle size of 60-80 mesh, respectively.

[0034] In a preferred embodiment, the Orostachys fimbriatus is ground into a powder with a particle size of 80-100 mesh.

[0035] In a preferred embodiment, the Mirabilis himalaica and the Tribulus terrestris are ground into a powder with a particle size of 100-120 mesh, respectively.

[0036] In the present application, it is understood by those skilled in the art that the processing object of the extraction step is the raw material processed by the above grinding step.

[0037] In the present application, the extraction method can include soaking, percolation or ultrasonic-assisted extraction; in the soaking method, the ground herbal ingredients are soaked in one or more solvents, and the bioactive compounds are released over time by gentle stirring to enhance the extraction efficiency; in the percolation method, the solvents pass through the ground herbal ingredients, dissolving the ground herbal ingredients and carrying away the bioactive compounds; in the ultrasonic-assisted extraction method, high-frequency vibrations are generated in the solvent using ultrasonic waves, increasing the penetration of the solvent into the ground herbal ingredients and improving the release of bioactive compounds.

[0038] In a preferred embodiment, the extraction method includes ultrasonic extraction; the power, temperature and time of the ultrasonic extraction are 600 W, 70℃ and 85 min, respectively; and the solid-liquid ratio of the ultrasonic extraction is 1:13 g / mL.

[0039] In the present application, the solid-liquid ratio of the ultrasonic extraction refers to the ratio of the mass of each raw material after the grinding step to the volume of the extraction liquid.

[0040] In a preferred embodiment, the extraction step includes primary extraction and secondary extraction.

[0041] In the present application, the extraction reagent of the extraction step is one or more of water, ethanol, methanol, water-ethanol mixture, supercritical fluid and ionic liquid.

[0042] Preferably, the reagent for the primary extraction includes purified water with a pH value of 6.8-7.2.

[0043] Preferably, the reagent for the secondary extraction includes a 60% ethanol solution.

[0044] In a preferred embodiment, the extraction step further includes gradient extraction.

[0045] Preferably, the reagent for the gradient extraction includes a 30% ethanol solution, a 60% ethanol solution and a 90% ethanol solution.

[0046] In the present application, ethanol solution is used for extraction; on the one hand, ethanol is beneficial to the extraction of flavonoids and phenolic substances; on the other hand, water extracts polysaccharides.

[0047] In the present application, the concentrating method can be vacuum evaporation, rotary evaporation or freeze concentration; wherein the vacuum evaporation includes using reduced pressure to lower the boiling point of the excess solvent, so that the solvent can be effectively removed at a lower temperature, thereby preserving the bioactive compounds; the rotary evaporation includes using rotation and controlled heat to gently evaporate the excess solvent, improving the separation of the excess solvent from the bioactive compounds and enhancing the concentration of the extract; the freeze concentration includes removing the frozen excess solvent by sublimation, thereby obtaining a more concentrated bioactive compound extract.

[0048] In a specific embodiment, the concentrating method includes vacuum concentration.

[0049] Preferably, the temperature and pressure of the vacuum concentration are 55°C and -0.08 MPa, respectively.

[0050] In the present application, the separation method can be liquid-liquid extraction separation, chromatography separation or membrane filtration separation. Wherein the liquid-liquid extraction separation separates bioactive compounds by using two immiscible liquids, in which the bioactive compounds are transferred from one phase to another based on solubility characteristics; the chromatography separation is a separation technique that separates bioactive compounds based on chemical affinity, size and charge differences using a stationary phase and a mobile phase; the membrane filtration separation involves separating components based on molecular weight and size by passing the bioactive compounds through a membrane with a specific pore size.

[0051] In a specific embodiment, the separation method includes resin separation.

[0052] Preferably, the resin of the resin separation includes ADS-8 resin and HPD-100 resin.

[0053] Preferably, the column parameters of the resin separation are: 1.5 cm x 30 cm glass column, flow rate 2 mL / min.

[0054] In the present application, the encapsulation can be spray coating, freeze encapsulation or microencapsulation. Wherein the spray coating involves spraying a liquid encapsulation material onto the separated bioactive compounds to form a protective layer, enhance stability and control the release of the bioactive compounds; the freeze encapsulation is configured to solidify the encapsulation material around the separated bioactive compounds, ensuring minimal degradation and maintaining efficacy during storage; the microencapsulation is a technique that encapsulates the separated bioactive compounds in tiny capsules, providing controlled release, improved stability and enhanced bioavailability by protecting the separated bioactive compounds from external factors such as light, heat and air.

[0055] In a specific embodiment, the encapsulation method includes spray drying encapsulation.

[0056] Preferably, the spray-dried encapsulated agent is maltodextrin.

[0057] Preferably, the spray-dried encapsulated agent has an inlet temperature of 160℃ and an outlet temperature of 85℃.

[0058] In a specific embodiment, the mixing is performed in a V-shaped mixer.

[0059] Preferably, the mixer has a rotation speed of 15 rpm.

[0060] Preferably, the mixing is performed for 30 minutes.

[0061] In a specific embodiment, the method further comprises a sieving step after the mixing step, wherein the sieving step comprises using a 100-mesh sieve.

[0062] In the present application, the herbal composition can improve cell survival rate, anti-apoptosis, ROS level, antioxidant enzyme activity (quantitative radical scavenging and enzyme activity improvement range), mitochondrial function indicators (ATP production, membrane potential maintenance), and long-lived gene expression.

[0063] The present application also provides a preparation comprising the herbal composition as described above.

[0064] In the present application, the dosage form of the preparation is one or more of tablets, capsules, powders, granules, suspensions, emulsions, gels, creams, and lotions.

[0065] On the basis of common general knowledge in the art, the above-mentioned preferred conditions can be combined arbitrarily, thereby obtaining various preferred examples of the present application.

[0066] The reagents and raw materials used in the present application are commercially available.

[0067] The positive progress effect of the present application is that:

[0068] 1. The herbal composition of the present application is beneficial for optimizing cell viability and anti-aging, and has a wide range of benefits in terms of anti-inflammatory, antioxidant, and adaptogenic effects, for supporting overall vitality, reducing stress, and enhancing immune function, with relatively low side effect risk, in a more comprehensive and balanced manner to support anti-aging;

[0069] 2. The herbal composition of the present application has the effects of enhancing cell regeneration, improving antioxidant biomarkers, regulating hormone balance, enhancing immune function, and improving cell energy production;

[0070] 3. The herbal composition of the present application helps to combat oxidative stress and promotes overall health, supports respiratory health and enhances the immune system, enhances stress resilience and overall vitality, with synergistic effects between the extracts, maximizing health benefits while maintaining optimal potency. DETAILED DESCRIPTION

[0071] The present application is further illustrated by the following examples, which are not intended to limit the application in any way. The experimental methods in the following examples, for which specific conditions are not specified, are selected in accordance with conventional methods and conditions, or according to the instructions of the commercial product.

[0072] In this document, the term "exemplary" is used to mean "serving as an example, instance, or illustration." Any implementation of the subject matter described herein is not necessarily considered to be "exemplary" solely based on its inclusion in this section.

[0073] The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a device or a subsystem or element or a structure or a component that is "comprised in" may include an additional device, subsystem, additional module, or element or structure or component that is not recited. The terms "in one embodiment", "in another embodiment", and similar language as used in this specification do not necessarily denote the same embodiment, unless otherwise specified.

[0074] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The systems, methods, and examples provided herein are illustrative only and not intended to be limiting.

[0075] Example 1

[0076] The present embodiment provides a herbal composition and preparation for optimizing cell vitality and anti-aging.

[0077] The herbal composition comprises: an extract of Polygonatum 30%, an extract of Asparagus 30%, an extract of Oreomyrrhis tibetica 15%, an extract of Mirabilis himalaica 15%, and an extract of Tribulus terrestris 10%; wherein % refers to the mass percentage content of each extract in the herbal composition.

[0078] The preparation method of the herbal composition comprises the following steps in sequence:

[0079] S1, raw materials of each extract are subjected to extraction treatment, and the raw materials are Polygonatum, Asparagus, Oreomyrrhis tibetica, Mirabilis himalaica, and Tribulus terrestris.

[0080] S2, each extract is further concentrated, separated, encapsulated, and mixed;

[0081] In step S1, the extraction process comprises the following steps in sequence:

[0082] ss1, harvesting the raw materials at the optimal maturity

[0083] The optimal maturity of Polygonatum sibiricum, Polygonatum sibiricum, Tibetan Eutrema japonicum and Himalayan Mirabilis is Polygonatum sibiricum and Polygonatum sibiricum in late autumn of the third year, Tibetan Eutrema japonicum and Himalayan Mirabilis at the flowering stage, and Tribulus terrestris at the stage of complete fruit development;

[0084] ss2, cleaning the harvested raw materials

[0085] The cleaning steps of Polygonatum sibiricum, Polygonatum sibiricum, Tibetan Eutrema japonicum, Himalayan Mirabilis and Tribulus terrestris each comprise in sequence: primary washing, secondary washing and final rinsing; the reagent for primary washing comprises purified water containing 0.5% sodium bicarbonate; the secondary washing method comprises ultrasonic cleaning, and the ultrasonic frequency and power are 40 kHz and 300 W respectively; the reagent for final rinsing is distilled water.

[0086] ss3, drying the raw materials treated by the cleaning step

[0087] The drying step comprises in sequence: cool drying and hot air drying; the temperature and time of cool drying are 25-30°C respectively; the temperature and time of hot air drying are 45°C and 8-12h respectively; the dried Polygonatum sibiricum has a water content of 10%, the dried Polygonatum sibiricum has a water content of 10%, the dried Tibetan Eutrema japonicum has a water content of 8%, the dried Himalayan Mirabilis has a water content of 6%, and the dried Tribulus terrestris has a water content of 6%.

[0088] ss4, grinding the dried raw materials

[0089] The dried Polygonatum sibiricum and Polygonatum sibiricum are ground into powders with a particle size of 60-80 mesh respectively; the dried Tibetan Eutrema japonicum is ground into a powder with a particle size of 80-100 mesh; the dried Himalayan Mirabilis and Tribulus terrestris are ground into powders with a particle size of 100-120 mesh respectively;

[0090] ss5, ultrasonic extraction of the ground raw materials

[0091] The power, temperature and time of ultrasonic extraction are 600 W, 70°C and 85 min respectively; the solid-liquid ratio of ultrasonic extraction is 1:13 g / mL; the extraction step comprises primary extraction and secondary extraction; the reagent for primary extraction comprises purified water with a pH value of 6.8-7.2; the reagent for secondary extraction comprises 60% ethanol solution;

[0092] In step S2, each extract is vacuum concentrated; the temperature and pressure of vacuum concentration are 55°C and -0.08 MPa respectively;

[0093] In step S2, the separation method includes resin separation; the resin of the resin separation includes ADS-8 resin and HPD-100 resin; the chromatographic column parameters of the resin separation are as follows: 1.5 cm x 30 cm glass column, flow rate 2 mL / min;

[0094] In step S2, the encapsulation method includes spray drying encapsulation; the reagent of the spray drying encapsulation is maltodextrin; the inlet temperature and outlet temperature of the spray drying encapsulation are 160°C and 85°C respectively;

[0095] In step S2, the mixing process is carried out in a V-type mixer; the rotation speed of the mixer is 15 rpm; the mixing time is 30 min;

[0096] In step S2, after the mixing step, there is also a step of sieving with a 100-mesh sieve.

[0097] The preparation of the embodiment includes the herbal composition as described above.

[0098] The herbal composition and preparation of the embodiment are beneficial for optimizing cell vitality and anti-aging, and have wide benefits in anti-inflammatory, antioxidant and adaptogenic effects, for supporting overall vitality, reducing stress and enhancing immune function, with relatively low side effect risk, to support anti-aging in a more comprehensive and balanced way.

[0099] Effect Example 1

[0100] The effect embodiment discloses specific effect advantages of Example 1 in optimizing cell vitality and anti-aging:

[0101] (1) Enhancing cell regeneration: the composition increases the cell proliferation rate in human fibroblast cell culture by 32%, and prolongs the cell lifespan by 25%.

[0102] (2) Improving antioxidant biomarkers: in animal models, the formula reduces malondialdehyde (MDA) levels by 45% and increases superoxide dismutase activity by 68%.

[0103] (3) Regulating hormone balance: the formula helps maintain optimal stress hormone levels, reducing cortisol levels by 38% in stress-induced animal models.

[0104] (4) Enhancing immune function: the composition significantly increases natural killer cell activity by 42% and macrophage phagocytosis by 35%, improving overall immune response.

[0105] (5) Improving cell energy production: the formula enhances mitochondrial function, increasing ATP production by 27% in a model of aging cells.

[0106] The language used in this specification has been chosen primarily for readability and instructional purposes and may not have been selected to define or limit the scope of the inventive subject matter. Therefore, it is intended that the scope of the invention is not limited by this detailed description, but rather by any claims published in the application based thereon. Accordingly, embodiments of the invention are intended to be illustrative, not restrictive, and the scope of the invention is set forth in the following claims.

Claims

1. An herbal composition for optimizing cell vitality and anti-aging, characterized in that, It comprises: 5%-80% extract of Polygonatum sibiricum, 5%-80% extract of Asparagus cochinchinensis, 5%-80% extract of Ostericum maximowiczianum, 5%-80% extract of Mirabilis himalaica, and 5%-80% extract of Tribulus terrestris; Wherein, % refers to the mass percentage of each extract in the herbal composition.

2. The herbal composition as claimed in claim 1, wherein, The herbal composition comprises: 25%-40% extract of Polygonatum sibiricum, 25%-40% extract of Asparagus cochinchinensis, 10%-20% extract of Ostericum maximowiczianum, 10%-20% extract of Mirabilis himalaica, and 10%-15% extract of Tribulus terrestris.

3. The herbal composition as claimed in claim 2, wherein, The herbal composition comprises: 30%-40% extract of Polygonatum sibiricum, 30%-40% extract of Asparagus cochinchinensis, 10%-15% extract of Ostericum maximowiczianum, 10%-15% extract of Mirabilis himalaica, and 10%-12% extract of Tribulus terrestris.

4. The herbal composition as claimed in claim 1, wherein, The preparation method of the herbal composition comprises the following steps in sequence: raw materials of each extract are respectively subjected to extraction treatment, and then each extract is subjected to concentration, separation, encapsulation and mixing; The raw materials comprise Polygonatum sibiricum, Asparagus cochinchinensis, Ostericum maximowiczianum, Mirabilis himalaica and Tribulus terrestris.

5. The herbal composition as claimed in claim 4, wherein, The extraction treatment comprises the following steps in sequence: The raw materials are harvested, and then subjected to cleaning, drying, grinding and extraction steps respectively to obtain each extract.

6. The herbal composition as claimed in claim 5, wherein, The cleaning step of each raw material comprises primary washing, secondary washing and final rinsing in sequence; The reagent for the primary washing comprises purified water containing 0.5% sodium bicarbonate; The secondary washing mode comprises ultrasonic washing, and the ultrasonic frequency and power of the ultrasonic washing are 40 kHz and 300 W respectively; The reagent for the final rinsing is distilled water.

7. The herbal composition as claimed in claim 5, wherein, The drying step of each raw material satisfies one or more of the following conditions: ①The drying step comprises shade drying and hot air drying in sequence; The temperature and time of the shade drying are 25-30°C respectively; The temperature and time of the hot air drying are 45°C and 8-12 h respectively; ②The water content at the end of the drying of the Polygonatum sibiricum is 8%-12%; ③The water content at the end of the drying of the Asparagus cochinchinensis is 8%-12%; ④The water content at the end of the drying of the Ostericum maximowiczianum is 6%-10%; ⑤The water content at the end of the drying of the Mirabilis himalaica is 5%-8%; ⑥The water content at the end of the drying of the Tribulus terrestris is 5%-8%.

8. The herbal composition as claimed in claim 5, wherein, The grinding and extraction steps of each extract satisfy one or more of the following conditions: ①The Polygonatum sibiricum and the Asparagus cochinchinensis are respectively ground into powders with a particle size of 60-80 mesh; ②The Ostericum maximowiczianum is ground into a powder with a particle size of 80-100 mesh; ③The Mirabilis himalaica and the Tribulus terrestris are respectively ground into powders with a particle size of 100-120 mesh; ④The extraction mode comprises ultrasonic extraction, and the power, temperature and time of the ultrasonic extraction are 600 W, 70°C and 85 min respectively; The solid-liquid ratio of the ultrasonic extraction is 1:13 g / mL; ⑤The extraction step comprises primary extraction and secondary extraction; The reagent for the primary extraction comprises purified water with a pH value of 6.8-7.2; The reagent of the secondary extraction includes 60% ethanol solution; The step of the extraction further includes gradient extraction; The reagent of the gradient extraction includes 30% ethanol solution, 60% ethanol solution and 90% ethanol solution.

9. The herbal composition as claimed in claim 4, wherein, The steps of the concentration, separation, encapsulation and mixing of each extract meet one or more of the following conditions: ① The concentration method includes vacuum concentration; the temperature and pressure of the vacuum concentration are 55℃ and -0.08MPa respectively; ② The separation method includes resin separation; the resin of the resin separation includes ADS-8 resin and HPD-100 resin; the column parameters of the resin separation are: 1.5cm×30cm glass column, flow rate 2mL / min; ③ The encapsulation method includes spray drying encapsulation; the reagent of the spray drying encapsulation is maltodextrin; the inlet temperature and outlet temperature of the spray drying encapsulation are 160℃ and 85℃ respectively; ④ The mixing process is carried out in a V-type mixer; the rotation speed of the mixer is 15rpm; the mixing time is 30min; ⑤ After the step of the mixing, a step of sieving is further included; the step of the sieving includes using a 100-mesh sieve.

10. A formulation characterized in that, It comprises the herbal composition according to any one of claims 1-9.