Core-shell structure up-conversion nano-composite, preparation method and application

By coating UCNPs with a Zr-MOF (UIO-66-NH2) shell and loading them with horseradish peroxidase (HRP), the drawbacks of silica coating on UCNPs were overcome, achieving high efficiency, biocompatibility, and stability of UCNPs in bioassays, and enhancing detection efficiency and functionalization capabilities.

CN121674050APending Publication Date: 2026-03-17SHANGHAI XINMIAO BIOLOGICAL TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411229256.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-03
Publication Date
2026-03-17

AI Technical Summary

Technical Problem

Existing UCNPs coated with silica suffer from issues such as lack of core particles and co-encapsulation, making it difficult to control the shell thickness, limiting biofunctionalization, and requiring additional chemical treatment, which affects their application in biosensing.

Method used

Horseradish peroxidase (HRP) was loaded onto a Zr-MOF (UIO-66-NH2) shell. The shell thickness was precisely controlled through a layer-by-layer self-assembly method. The amino and phosphate groups provided by the MOF shell were used for biofunctionalization modification to prepare the UCNP@MOF/HRP probe.

Benefits of technology

This study achieves high biocompatibility and stability of UCNPs, improves the specificity and detection efficiency of biological assays, and provides catalytic colorimetric function.

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Abstract

The invention discloses an up-conversion nano-composite with a core-shell structure, a preparation method and application, and belongs to the technical field of biomedical bimodal nano-probes. According to the nanocomposite, up-conversion nanoparticles (UCNPs) serve as a core, metal-organic frameworks (MOFs) serve as a shell layer, horse radish peroxidase (HRP) is loaded on the shell layer, and UCNP (at) MOF / HRP is formed. Subsequent biological functional modification, such as coupling of an antibody, an antigen, nucleic acid and other substances, can be carried by an activating group or a metal coordination bond carried by a shell ligand of the upconversion nanocomposite MOF with the core-shell structure, so that the functions of specific recognition, targeted capture, specific loading and the like are realized.
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