Enzymatic reactor

Abstract

The invention discloses an enzymatic reactor, which comprises a sealed cavity, a reaction kettle, a reaction kettle and a control system, and the sealed cavity is internally provided with an accommodating space for accommodating reactants; the temperature control module is attached to the outer side surface of the sealing cavity; the weighing module is used for acquiring the mass of the contained reactants; the stirring module comprises a stirring part and a driving part, the stirring part is arranged in the containing space, the driving part is arranged outside the containing space, and the driving part drives the stirring part to rotate in an induction driving mode. According to the enzymatic reactor, the heating efficiency can be improved, the heating time requirement of enzymatic reaction can be met without heating of other liquid heat transfer media, the cleanliness degree is high, and the size is small. The mass of the reactant in the sealed cavity is obtained through the weighing module so as to be used for weighing and metering of the reaction system configuration, the reaction precision can be improved, the driving part of the stirring module is not in contact with the reactant, and the enzymatic reaction is prevented from being influenced.

Description

Technical Field

[0001] This invention relates to an enzymatic reactor. Background Technology

[0002] Due to the rapid development of the mRNA vaccine field, the demand for large-scale enzymatic reaction preparation is gradually increasing. Enzymatic reaction preparation needs to meet GMP standards, and there are high requirements for reactant temperature control, feed accuracy, reactant weighing accuracy, and agitation. Therefore, currently there is no equipment capable of scaling up enzymatic reactions from pilot-scale to industrial production, and various pilot-scale equipment cannot meet the needs of industrial production.

[0003] For example, the equipment currently available for pilot-scale enzymatic reactions typically falls into three categories: high-precision constant-temperature water baths, wave-type bioreactors, and fermenter-type enzymatic reaction systems. The disadvantages of these devices are explained below:

[0004] Firstly, while high-precision constant-temperature water baths offer high temperature control accuracy, reaching 0.03℃, the limitations of water baths prevent stirring, requiring manual shaking for mixing during the reaction, and all material replenishment must be done manually. Therefore, they are only suitable for early-stage research and development and related phases, and lack the capability for large-scale production.

[0005] Secondly, wave reactors based on cell production, possessing similar functions, have also been attempted for application in enzymatic reaction production processes. However, in practical use, it has been found that: due to the long cell production cycle (over 12 hours), wave reactors employ a single-sided heating mode, with only a heating block at the bottom of the reactor. If the power is too high, the bottom temperature will overheat, exceeding 40°C, causing inactivation of the bioreactive enzymes and production failure. Conversely, reducing the power results in excessively long heating times; the typical reaction time for enzymatic reactions is 1-2 hours, while the heating time for wave reactors can reach up to 2 hours, affecting yield. Furthermore, because their feeding system is developed based on cell feeding, the related design precision is relatively low and the pipelines are relatively thick, leading to significant waste of relatively expensive enzymatic reaction raw materials.

[0006] Finally, fermenter-type enzymatic reaction systems are developed based on glass fermenters. These reaction devices are developed with reference to bacterial fermentation production equipment, resulting in poor compatibility with enzymatic reactions, excessive stirring shear forces, and a rigid connection between the agitator and the top of the fermenter, requiring integrated offline sterilization. They also carry the risk of cross-contamination between different species. Furthermore, these devices typically require an external TCU for temperature control, have built-in condensate media requiring regular cleaning, and are bulky, making them difficult to use at GMP levels. Moreover, like the aforementioned wave reactors, they suffer from relatively coarse feeding, significant loss of raw materials for enzymatic reactions, and lack the potential for further automated production management. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to overcome the shortcomings of low yield and high operation difficulty of enzymatic reactions using existing equipment, and to provide an enzymatic reactor.

[0008] The present invention solves the above-mentioned technical problems through the following technical solution:

[0009] An enzymatic reactor comprising:

[0010] A sealed cavity, the interior of which has a containment space for accommodating reactants;

[0011] A temperature control module, comprising a temperature regulating part and a temperature detection part, wherein the temperature regulating part is attached to the outer surface of the sealed cavity, and the temperature detection part is located within the accommodating space;

[0012] A weighing module, the weighing module being used to obtain the mass of the reactants contained therein;

[0013] The stirring module includes a stirring part and a driving part. The stirring part is disposed within the receiving space, and the driving part is disposed outside the receiving space. The driving part drives the stirring part to rotate by means of induction.

[0014] This enzyme-catalyzed reactor uses a sealed cavity to contain the reactants of the enzyme-catalyzed reaction, thus isolating the reactants from the outside air flow. Compared with the non-fully temperature-controlled closed reaction tanks currently used in preparation, it can improve heating efficiency and meet the temperature rise time requirements of the enzyme-catalyzed reaction without the need for other liquid heat transfer media. It also has a high degree of cleanliness and small size.

[0015] By placing the temperature adjustment part of the temperature control module on the outer surface of the sealed cavity, and placing the temperature detection part inside the containment space of the sealed cavity, the temperature detection response speed of the reactants can be improved, so as to reach the target temperature more quickly.

[0016] The system includes a weighing module and a stirring module. The weighing module obtains the mass of the reactants inside the sealed cavity for weighing and measuring the reaction system, thereby improving the accuracy of the reaction.

[0017] Simultaneously, the stirring unit located within the sealed cavity is driven to rotate via induction, achieving stirring while isolating the reactants from the external environment. Furthermore, the driving unit of the stirring module does not come into contact with the reactants, thus avoiding interference with the enzymatic reaction.

[0018] Therefore, this enzyme reactor can meet the requirements of industrial production of enzyme-catalyzed reactions in terms of temperature control efficiency and stirring capacity.

[0019] Preferably, the weighing module indirectly obtains the mass of the contained reactants by obtaining the mass of the entire sealed cavity.

[0020] This weighing module indirectly obtains the mass of the contained reactants by acquiring the mass of the entire sealed cavity, providing a relatively simple and reliable solution for obtaining the mass of the internal reactants. Compared with other methods of obtaining mass, it can simplify the structure of the enzyme reactor and is beneficial to meeting the high environmental cleanliness requirements of enzyme-catalyzed reactions.

[0021] Preferably, the enzyme reactor further includes a base, the upper surface of which is used to support the sealed cavity, and the weighing sensor of the weighing module is integrated on the upper surface of the base and directly contacts the bottom surface of the sealed cavity.

[0022] The weighing module obtains the mass of the reactants contained in the sealed cavity by carrying the sealed cavity and weighing it. Compared with other detection schemes, it has a simpler structure and a higher degree of integration.

[0023] Preferably, the drive unit is integrated into the base and located near the upper surface of the base;

[0024] The drive unit is positioned within the base corresponding to the center of the sealed cavity, and the weighing sensor is arranged circumferentially around the drive unit.

[0025] This structural design allows the weighing sensor of the weighing module and the drive unit of the stirring module to be arranged simultaneously at the bottom of the sealed cavity, without interference between the weighing sensor and the drive unit, and the drive unit can effectively drive the stirring unit to rotate.

[0026] Preferably, the detection accuracy of the weighing sensor is less than or equal to 1g. This structural design can effectively meet the weighing accuracy requirements of enzymatic reactions.

[0027] Preferably, the enzymatic reactor further includes a feeding module, which uses a servo motor as a power source.

[0028] By using a feeding module powered by a servo motor, feeding accuracy can be improved and material loss reduced compared to the current solution that uses a peristaltic pump.

[0029] Preferably, the enzyme reactor further includes an automated control module, which is connected to a servo motor signal.

[0030] By using an automated control module to signal-control the servo motor of the feeding module to turn it on or off, the feeding accuracy and the automation level of the entire enzyme reactor can be improved.

[0031] Preferably, the feeding module further includes an electronic balance, the detection accuracy of which is less than or equal to 1g;

[0032] Preferably, the pipeline connecting the feeding module and the sealed cavity is a pressure-resistant thin tube.

[0033] Preferably, the temperature detection unit is an insertion thermometer that is matched with the material bag of the reactants.

[0034] By using an insertion thermometer that is compatible with the reactant bag as the temperature detection unit, the accuracy and speed of obtaining the current actual temperature of the reactant can be improved. This allows for more precise temperature control of the temperature regulating unit based on the current actual temperature, enabling the reactant to reach the target temperature more quickly.

[0035] Preferably, there are multiple temperature regulating units, which are distributed on different outer surfaces of the sealed cavity.

[0036] By arranging multiple temperature control units and distributing them on different outer surfaces of the sealed cavity, the uniformity of temperature control is improved to meet the temperature uniformity requirements of the enzymatic reaction.

[0037] Preferably, the temperature regulating unit uses a semiconductor thermostat or electric heating for temperature control.

[0038] Compared to other temperature control devices, using semiconductor thermostats offers higher temperature control precision, which is beneficial for meeting the high cleanliness requirements of enzymatic reactions. Furthermore, the simple structure of semiconductor thermostats allows for the placement of multiple thermostats on different outer surfaces of the sealed cavity to improve temperature uniformity. Meanwhile, electric heating is highly efficient, with a faster heating rate, enabling the target temperature to be reached more quickly.

[0039] Preferably, the temperature control range of the semiconductor thermostat or electric heating element is at least 30°C to 40°C.

[0040] This structural design can effectively adapt to the temperature control range of enzyme-catalyzed reactions.

[0041] Preferably, the temperature control accuracy of the semiconductor thermostat is less than or equal to 1°C. This structural design effectively adapts to the temperature control accuracy range of enzymatic reactions.

[0042] Preferably, the shape of the receiving space of the sealed cavity is cuboid, and the outer surface of the sealed cavity for attaching the temperature regulating part is planar.

[0043] By adopting a cuboid shape as the shape of the sealed cavity, on the one hand, the outer surface of the sealed cavity can provide a flat surface for the temperature regulation unit to be attached. The structure is simple and can ensure a large area of ​​contact between the temperature regulation unit and the sealed cavity, thereby improving the temperature control effect and accuracy. On the other hand, it is also suitable for the shape of the reactant strip.

[0044] Preferably, the enzyme reactor further includes an outer casing that covers both the sealed cavity and the outer surface of the temperature regulating unit.

[0045] This enzyme-catalyzed reactor, by setting an outer shell to simultaneously cover the outer surfaces of the sealed cavity and the temperature regulation unit, further reduces the impact of external environmental temperature changes on the sealed cavity and the temperature regulation unit, further improves the temperature control efficiency of the temperature regulation unit on the sealed cavity, and meets the needs of industrial production of enzyme-catalyzed reactions.

[0046] Preferably, both the outer casing and the sealed cavity are made of stainless steel.

[0047] The outer casing and sealed cavity are made of stainless steel to meet the high environmental cleanliness requirements of the enzymatic reaction site.

[0048] Preferably, the outer casing and the sealed cavity are integrated.

[0049] By integrating the outer casing with the sealed cavity, manufacturing is easier and insulation performance is better.

[0050] Preferably, the volume of the containment space is defined as V1, and the volume of the reactants contained in the sealed cavity is defined as V2, with the value of volume V1 ranging from 1.1V2 to 1.3V2.

[0051] By controlling the volume of the containment space between 1.1 and 1.3 times the volume of the reactants, it is possible to avoid excessively large containment space that could cause temperature fluctuations, while also avoiding excessively small containment space that would not be able to effectively contain the reactants.

[0052] Preferably, the driving unit of the stirring module drives the stirring unit to rotate via magnetic induction.

[0053] Compared to other induction-driven solutions, magnetic induction-driven technology is more mature and outputs less shear force, which can effectively avoid excessive stirring of the reactants.

[0054] Preferably, the driving unit of the stirring module can also adjust the height of the stirring unit within the accommodating space by means of inductive driving.

[0055] By using a sensor-driven mechanism to adjust the height of the stirring unit within the containment space, the stirring requirements during the enzymatic reaction process can be further met by adjusting the height of the stirring unit. On the other hand, it eliminates the need for a separate mechanical structure within the containment space to adjust the height of the driving unit, thus avoiding any impact on the enzymatic reaction within the containment space caused by additional mechanical structures.

[0056] The positive and progressive effects of this invention are as follows:

[0057] This enzymatic reactor improves heating efficiency and meets the temperature rise time requirements of enzymatic reactions without the need for additional liquid heat transfer media. It also boasts high cleanliness and a compact size. A weighing module acquires the mass of reactants within the sealed chamber for weighing and metering the reaction system, improving reaction accuracy. Simultaneously, the drive unit of the stirring module does not contact the reactants, avoiding interference with the enzymatic reaction. Therefore, this enzymatic reactor meets the requirements for industrial-scale enzymatic reaction production in terms of temperature control efficiency and stirring capability. Attached Figure Description

[0058] Figure 1 This is a schematic diagram of an enzyme reactor module provided in a preferred embodiment of the present invention.

[0059] Figure 2 This is a schematic diagram of the sealed cavity of the enzyme reactor and the temperature adjustment section of the temperature control module provided in a preferred embodiment of the present invention.

[0060] Figure 3 This is a top view of a base provided in a preferred embodiment of the present invention.

[0061] Explanation of reference numerals in the attached figures:

[0062] Enzyme reactor 100

[0063] Sealed cavity 1, containing space 11

[0064] Temperature control unit 2

[0065] Weighing Module 3

[0066] Stirring module 4, stirring part 41, driving part 42

[0067] Base 5

[0068] Outer shell 6

[0069] Feeding module 200

[0070] Automation control module 300 Detailed Implementation

[0071] The present invention will be further illustrated by way of embodiments below, but the present invention is not limited to the scope of the embodiments described herein.

[0072] The preparation of enzyme-catalyzed reactions requires compliance with GMP standards, and high requirements are placed on reactant temperature control, feed accuracy, reactant weighing accuracy, and agitation. Currently, there are no commercially available enzyme-catalyzed reaction devices specifically designed to meet the specific requirements of enzyme-catalyzed reactions. Therefore, existing equipment cannot meet the need to scale up enzyme-catalyzed reactions from pilot-scale to industrial production.

[0073] To address this problem, the present invention provides an enzyme-catalyzed reactor 100, specifically comprising a sealed cavity 1, a temperature control module, a weighing module 3, a stirring module 4, a feeding module 200, and an automated control module 300. For example... Figure 2 As shown, the sealed cavity 1 has an internal space 11 for accommodating the reactants of the enzymatic reaction. The temperature control module includes a temperature regulating unit 2 and a temperature detection unit (not shown in the figure). The temperature regulating unit 2 is attached to the outer surface of the sealed cavity 1, while the temperature detection unit is located inside the accommodating space to detect the temperature within the accommodating space. Based on the detected temperature, the temperature of the temperature regulating unit 2 is controlled to achieve actual temperature control. Using a sealed cavity 1 to accommodate the reactants of the enzymatic reaction isolates the reactants from external airflow. Compared to the currently used non-fully temperature-controlled sealed reaction vessels, this method improves heating efficiency, eliminates the need for other liquid heat transfer media to meet the temperature rise time requirements of the enzymatic reaction, and offers high cleanliness and small size.

[0074] like Figure 1 As shown, the weighing module 3 is used to obtain the mass of the contained reactants, while the stirring module 4 includes a stirring part 41 and a driving part 42. The stirring part 41 is disposed inside the containing space 11, and the driving part 42 is disposed outside the containing space 11. The driving part 42 drives the stirring part 41 to rotate via induction. By setting up the weighing module 3 and the stirring module 4, the weighing module 3 obtains the mass of the reactants in the sealed cavity 1 for weighing and measuring the reaction system, thereby improving the accuracy of the reaction. At the same time, the stirring part 41 located in the containing space 11 of the sealed cavity 1 is driven to rotate via induction, so as to achieve stirring while isolating the reactants from the outside environment. Meanwhile, the driving part 42 of the stirring module 4 does not come into contact with the reactants, avoiding interference with the enzymatic reaction.

[0075] like Figure 1 As shown, the feeding module 200 is used to feed materials into the sealed cavity 1, and the automation control module 300 is used to realize automation control.

[0076] In this embodiment, the weighing module 3 indirectly obtains the mass of the contained reactants by acquiring the mass of the entire sealed cavity 1, providing a relatively simple and reliable solution for obtaining the mass of the internal reactants. Compared with other methods of obtaining mass, this simplifies the structure of the enzyme reactor 100 and helps to meet the high environmental cleanliness requirements of the enzyme reaction site.

[0077] In this embodiment, the temperature detection unit is an insertion thermometer that is matched with the reactant bag to improve the accuracy and speed of obtaining the current actual temperature of the reactants. This allows for more precise temperature control of the temperature regulating unit based on the current actual temperature, enabling the reactants to reach the target temperature more quickly. Of course, in other embodiments, the temperature detection unit can also use any sensor capable of obtaining the current actual temperature of the reactants to achieve the temperature measurement purpose.

[0078] Specifically, such as Figure 1 As shown, the enzyme-catalyzed reactor 100 also includes a base 5. The upper surface of the base 5 supports the sealed cavity 1. The weighing sensor of the weighing module 3 is integrated on the upper surface of the base 5 and directly contacts the bottom surface of the sealed cavity 1. In this structural arrangement, the weighing module 3 obtains the mass of the reactants contained in the sealed cavity 1 by supporting the sealed cavity 1 and weighing it. Compared with other detection schemes, this method has a simpler structure and a higher degree of integration.

[0079] At the same time, such as Figure 1 and 3 As shown, in this embodiment, the drive unit 42 of the stirring module 4 is also integrated into the base 5 and is located relatively close to the upper surface of the base 5, so as to be closer to the stirring unit 41 inside the sealed cavity 1, thereby improving the sensing and driving capability. Specifically, in this embodiment, the drive unit 42 is set at the center position of the sealed cavity 1, and the weighing sensor is arranged around the drive unit 42 in the circumferential direction.

[0080] This structural arrangement allows the weighing sensor of the weighing module 3 and the drive unit 42 of the stirring module 4 to be arranged at the bottom of the sealed cavity 1 simultaneously, and the weighing sensor and the drive unit 42 do not interfere with each other, while the drive unit 42 can effectively drive the stirring unit 41 to rotate.

[0081] Specifically, the detection accuracy of the weighing sensor should be less than or equal to 1g to effectively meet the weighing accuracy requirements of enzyme-catalyzed reactions.

[0082] like Figure 2As shown, in this embodiment, the receiving space 11 of the sealed cavity 1 is rectangular, and the outer surface of the sealed cavity 1 for the temperature adjustment part 2 of the temperature control module to be attached is flat. By using a rectangular shape as the shape of the receiving space 11 of the sealed cavity 1, on the one hand, the outer surface of the sealed cavity 1 can provide a flat surface for the temperature adjustment part 2 to be attached, the structure is simple and can ensure a large area of ​​contact between the temperature adjustment part 2 and the sealed cavity 1, improving the temperature control effect and accuracy. On the other hand, it is also more suitable for the shape of the reactant strip. Of course, in other embodiments, the shape of the receiving space 11 of the sealed cavity 1 can also be set according to actual needs, such as using a cylindrical or polygonal shape.

[0083] Meanwhile, the number of temperature regulating units 2 is also set to four corresponding to the four sides of the rectangular shape of the sealed cavity 1, and they are respectively distributed on the four outer surfaces of the sealed cavity 1 to improve the uniformity of temperature control and meet the temperature uniformity requirements of the enzymatic reaction.

[0084] In this embodiment, the temperature regulating unit 2 uses a semiconductor thermostat for temperature control. Compared to other temperature control devices, using a semiconductor thermostat offers higher precision, which is beneficial for meeting the high environmental cleanliness requirements of enzymatic reactions. Furthermore, the semiconductor thermostat has a simple structure, allowing for the placement of multiple thermostats on different outer surfaces of the sealed cavity 1 to improve temperature uniformity. Of course, in other embodiments, other existing temperature regulating units 2 that meet the temperature control requirements can also be used to control the temperature of the sealed cavity 1. In other embodiments, electric heating can also be used for temperature control. Under the same temperature regulating unit size constraints, electric heating offers relatively higher heating efficiency, faster heating rate, and can reach the target temperature more quickly.

[0085] Specifically, the temperature control range of the semiconductor thermostat should be at least 30℃ to 40℃, and the temperature control accuracy of the semiconductor thermostat should be less than or equal to 1℃ in order to effectively adapt to the temperature control range of the enzyme-catalyzed reaction.

[0086] The enzyme-catalyzed reactor 100 also includes an outer casing 6, which simultaneously covers the outer surfaces of the sealed cavity 1 and the temperature regulating unit 2. By providing the outer casing 6 to simultaneously cover the outer surfaces of both the sealed cavity 1 and the temperature regulating unit 2, the influence of external environmental temperature changes on the sealed cavity 1 and the temperature regulating unit 2 is further reduced, and the temperature control efficiency of the temperature regulating unit 2 on the sealed cavity 1 is further improved, meeting the needs of industrial production of enzyme-catalyzed reactions. In this embodiment, as shown... Figure 2 As shown, the outer shell 6 and the sealed cavity 1 are integrated, which facilitates manufacturing and provides better insulation. Both the outer shell 6 and the sealed cavity 1 are made of stainless steel to meet the high environmental cleanliness requirements of the enzymatic reaction site.

[0087] Regarding the volume of the containment space 11 of the sealed cavity 1, if the volume of the containment space 11 is V1 and the volume of the contained reactants is V2, then the value of the volume V1 of the containment space 11 should be between 1.1V2 and 1.3V2 to avoid excessively large volumes of the containment space 11 leading to temperature fluctuations, and to avoid excessively small volumes of the containment space 11 failing to effectively contain the reactants. Specifically, in this embodiment, if the volume of the contained reactants is 1L, then the volume of the containment space 11 of the sealed cavity 1 can be designed according to a specification of 1.2L. If the volume of the contained reactants is 5L, then the volume of the containment space 11 of the sealed cavity 1 can be designed according to a specification of 6L.

[0088] In addition, in this embodiment, the driving unit 42 of the stirring module 4 drives the stirring unit 41 to rotate via magnetic induction. Compared with other induction driving schemes, magnetic induction driving technology is more mature and outputs relatively smaller shear force, which can effectively avoid over-stirring of the reactants.

[0089] Meanwhile, the drive unit 42 of the stirring module 4 can also adjust the height of the stirring unit 41 in the accommodating space 11 by means of induction drive. On the one hand, by adjusting the height position of the stirring unit 41, the stirring requirements in the enzymatic reaction process can be further met. On the other hand, it eliminates the need to set up a mechanical structure in the accommodating space 11 for adjusting the height of the drive unit 42, thus avoiding the impact of setting up an additional mechanical structure on the enzymatic reaction in the accommodating space 11.

[0090] Of course, in other embodiments, any existing stirring module 4 can be used to achieve non-contact stirring. At the same time, other non-magnetic induction methods can be used to adjust the stirring height.

[0091] In this embodiment, the feeding module 200 of the enzyme reactor 100 uses a servo motor as its power source. Feeding the enzyme reactor 100 with a servo motor improves feeding accuracy and reduces material loss compared to the current method using a peristaltic pump. Specifically, the feeding module 200 also includes an electronic balance with a detection accuracy of 1g or less. Furthermore, the automation control module 300 is connected to the servo motor signal to control its operation, thereby improving feeding accuracy and the overall automation level of the enzyme reactor 100.

[0092] This enzyme-catalyzed reactor 100 improves heating efficiency and meets the temperature rise time requirements of enzyme-catalyzed reactions without the need for other liquid heat transfer media. It also boasts high cleanliness and a small size. The mass of reactants within the sealed chamber 1 is obtained through the weighing module 3 for weighing and metering the reaction system, improving reaction accuracy. Simultaneously, the drive unit 42 of the stirring module 4 does not contact the reactants, avoiding interference with the enzyme-catalyzed reaction. Therefore, the enzyme-catalyzed reactor 100 meets the requirements for industrial-scale enzyme-catalyzed production in terms of temperature control efficiency and stirring capability.

[0093] The enzyme reactor 100 features high-precision uniform temperature control (temperature range of 30-40℃, temperature control accuracy ±1℃), high-precision feeding (accuracy 0.1g), high-precision weighing (accuracy 1g), and low-shear stirring function. Furthermore, it meets the requirements for large-scale enzyme-catalyzed reactions while complying with GMP standards, enabling the industrial production of enzyme-catalyzed reactions.

[0094] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. An enzyme-catalyzed reactor, characterized in that, It includes: A sealed cavity, the interior of which has a containment space for accommodating reactants; A temperature control module, comprising a temperature regulating part and a temperature detection part, wherein the temperature regulating part is attached to the outer surface of the sealed cavity, and the temperature detection part is located within the accommodating space; A weighing module, the weighing module being used to obtain the mass of the reactants contained therein; The stirring module includes a stirring part and a driving part. The stirring part is disposed within the receiving space, and the driving part is disposed outside the receiving space. The driving part drives the stirring part to rotate by means of induction.

2. The enzymatic reactor as described in claim 1, characterized in that, The weighing module indirectly obtains the mass of the contained reactants by acquiring the mass of the entire sealed cavity.

3. The enzymatic reactor as described in claim 2, characterized in that, The enzyme reactor also includes a base, the upper surface of which is used to support the sealed cavity. The weighing sensor of the weighing module is integrated on the upper surface of the base and directly contacts the bottom surface of the sealed cavity.

4. The enzymatic reactor as described in claim 3, characterized in that, The drive unit is integrated into the base and located near the upper surface of the base; The drive unit is positioned within the base corresponding to the center of the sealed cavity, and the weighing sensor is arranged circumferentially around the drive unit.

5. The enzymatic reactor as described in claim 3, characterized in that, The detection accuracy of the weighing sensor is less than or equal to 1g.

6. The enzymatic reactor as described in claim 1, characterized in that, The enzyme-catalyzed reactor also includes a feeding module, which uses a servo motor as its power source.

7. The enzymatic reactor as described in claim 6, characterized in that, The enzyme reactor also includes an automated control module, which is connected to a servo motor signal.

8. The enzymatic reactor as described in claim 6, characterized in that, The feeding module also includes an electronic balance, the detection accuracy of which is less than or equal to 1g; And / or, the pipeline connecting the feeding module to the sealed cavity is a pressure-resistant thin tube.

9. The enzymatic reactor as described in claim 1, characterized in that, The temperature detection unit is an insertion thermometer that is matched with the material bag of the reactants.

10. The enzymatic reactor according to claim 1, characterized in that, The temperature regulating units are multiple and are distributed on different outer surfaces of the sealed cavity.

11. The enzymatic reactor as described in claim 10, characterized in that, The temperature control unit uses a semiconductor thermostat or electric heating to control the temperature.

12. The enzymatic reactor as described in claim 11, characterized in that, The temperature control range of the semiconductor thermostat or electric heating element is at least 30°C to 40°C. And / or, the temperature control accuracy of the semiconductor thermostat is less than or equal to 1°C.

13. The enzymatic reactor as described in claim 10, characterized in that, The sealed cavity has a rectangular parallelepiped shape, and the outer surface of the sealed cavity for attaching the temperature regulating part is a plane.

14. The enzymatic reactor as described in claim 13, characterized in that, The enzyme reactor also includes an outer casing, which covers both the outer surface of the sealed cavity and the temperature control unit.

15. The enzymatic reactor as described in claim 14, characterized in that, Both the outer casing and the sealed cavity are made of stainless steel. And / or, the outer casing and the sealed cavity are integrally formed.

16. The enzymatic reactor according to any one of claims 1-15, characterized in that, The volume of the containment space is defined as V1, and the volume of the reactants contained in the sealed cavity is defined as V2. The value of volume V1 is between 1.1V2 and 1.3V2.

17. The enzymatic reactor according to any one of claims 1-15, characterized in that, The driving unit of the stirring module drives the stirring unit to rotate via magnetic induction.

18. The enzymatic reactor as described in claim 17, characterized in that, The driving unit of the stirring module can also adjust the height of the stirring unit within the accommodating space by means of induction driving.

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