Application of pleurotus sajor-caju in biotransformation and degradation

Abstract

The invention belongs to the field of microbial biotransformation and degradation technologies and fermentation engineering thereof, and particularly relates to application of pleurotus sajor-caju in biotransformation and degradation. According to the invention, Pleurotus sajor-caju MT is combined with wood pulp sponge to prepare a product capable of rapidly degrading the corpses, the Cu and Cd contents of the corpses and the surrounding environment of the corpses can be reduced in the degradation process, the product has the advantages of ideal degradation time, high public acceptance degree, less peculiar smell and the like, and meanwhile, the surrounding soil and environment can be effectively improved; and benign win-win of surrounding animals and plants is promoted. Particularly, a macro fungus degradation product taking wood pulp sponge as a framework has the price advantage, the production period of the product is shorter, expensive compression molding equipment does not need to be customized, the pollution rate of product culture is reduced, and the application prospect is wide.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of microbial bioconversion and degradation technology and fermentation engineering, and particularly relates to application of Pleurotus sajor-caju in bioconversion and degradation. BACKGROUND

[0002] According to the Pet Funeral Consumer Alliance, 246,240 tons of carbon dioxide are released annually due to animal cremation, equivalent to 41,040 cars, posing a huge challenge to the natural and human living environment. In terms of domestic, there are as many as 200 million pets in China at present, and pets generally undergo cremation after death. The energy consumption required by cremation equipment is huge, and the waste generated by it pollutes the air and environment to a certain extent. In addition, more than 95% of the 9,000 pet funeral and burial processing companies do not comply with regulations and have no qualifications. The domestic pet funeral industry is chaotic, but has great development potential. Loop Living Cocoon™ is the first biodegradable coffin for human corpses in the world, which is made of local mushroom varieties in the Netherlands and upgraded hemp fibers, and can biodegrade in 45 days and return to nature in the most natural way to enrich nutrients, proving the strong potential of fungal bioconversion and degradation in handling human and animal remains. However, the production of the above Loop Living Cocoon™ has the problems of expensive complete equipment, complicated operation, high product pollution rate, and high product price, and there is no reported fungus and related products that can be used for animal degradation in China, which needs to be filled in the domestic blank. SUMMARY

[0003] In order to obtain a new product for corpse degradation, the following technical solutions are provided in the application:

[0004] The application discloses application of Pleurotus sajor-caju MT in bioconversion and degradation, and the Pleurotus sajor-caju MT has a preservation number of CCTCC M 2021011.

[0005] Preferably, the bioconversion and degradation is corpse degradation.

[0006] Preferably, the bioconversion and degradation is animal corpse degradation.

[0007] Preferably, the bioconversion and degradation is pet corpse degradation.

[0008] Preferably, the specific method of the application is that the Pleurotus sajor-caju MT is covered around the corpse. Specifically, the specific method of the application is that:

[0009] (1) Pleurotus sajor-caju MT mycelium preparation:

[0010] First, prepare the liquid medium: 200-220g peeled potatoes, 8-10g protein peptone, 20-22g glucose, and add water to 1L; then inoculate Pleurotus sajor-caju MT into the liquid medium and vortex for 20-30min, then culture at 24-26℃, 160-200r / min for 1-2 days, then stand at the same temperature for 2-4h, then culture at 24-26℃, 60-100r / min for 4-5 days, to obtain Pleurotus sajor-caju MT;

[0011] (2) Cover the Pleurotus sajor-caju MT mycelium on the surface of the wood pulp sponge, and then wrap the corpse with the wood pulp sponge for degradation.

[0012] Preferably, the bioconversion and degradation are to reduce the Cu and Cd content in the remains and soil during the degradation of the corpse.

[0013] Advantages of the present application:

[0014] The present application combines Pleurotus sajor-caju MT with wood pulp sponge to prepare a product that can quickly degrade the corpse, and can reduce the Cu and Cd content of the corpse and the environment around the corpse during the degradation process, has the advantages of ideal degradation time, high public acceptance, less odor, etc., and can also effectively improve the surrounding soil and environment, and promote the benign coexistence of plants and animals. Especially, the large-scale fungal degradation product with wood pulp sponge as the skeleton has the advantage of price, the production cycle is shorter, and there is no need to customize expensive compression mold equipment, and the pollution rate of product culture is reduced, which has a broad application prospect. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0016] The application aims to expand the application range of the relatively mature wood pulp sponge product in China, to manufacture a biodegradable coffin that can be used for degrading pet corpses by using the screened large fungus strains with the wood pulp sponge carrier, and to greatly simplify the production process of the same product in the international market, to reduce the sterile requirements of product production and to reduce the product price. The product can be designed as a hollow container with different shapes such as a cylinder or a cuboid. When used, the pet corpse is only needed to be placed in the container and covered with a lid, and then placed in a cool environment to complete the natural degradation. The large fungus block obtained by the application can be effectively used for pet corpse degradation, and the natural degradation is completed in about 45 days. The treatment process does not require professional operation, is simple to use, and the degradation process is safe, odorless, environmentally friendly, and even can effectively improve the surrounding soil environment. The energy consumption is reduced and the environment is protected without cremation treatment.

[0017] The process for preparing a cylindrical or cuboid degradation product includes the following steps:

[0018] Strain screening: according to the growth of the strain on the wood chip culture medium plate (mycelium growth rate, concentration white degree, degradation condition), a strain of Pleurotus sajor-caju is screened as the material. The strain is obtained by tissue separation method and named Pleurotus sajor-caju MT. The source is a heavy metal contaminated abandoned edible fungus cultivation base in Changsha, Hunan Province. The strain is also preserved in China Typical Culture Collection Center, Wuhan University, Wuhan, China, with the preservation number CCTCC M 2021011, which has been disclosed in CN112852640A.

[0019] 2, liquid medium formula (1L calculation): peeled potato 200-220g, protein peptone 8-10g, glucose 20-22g, add water to 1L.

[0020] 3, Pleurotus sajor-caju liquid mycelium preparation: add 5-7 pieces of soybean-sized mycelium blocks on the above-mentioned plate to each 1L of culture solution, vortex for 20~30min, then culture at 24~26℃, 160~200r / min for 1~2 days, then stand at the same temperature for 2~4h, then culture at 24~26℃, 60~100r / min for 4~5 days, and obtain Pleurotus sajor-caju liquid mycelium.

[0021] 4, mixing: place the clean hollow cylindrical or cuboid wood pulp sponge (cylinder: after absorbing water, diameter 35 cm / high 40 cm / thickness 4 cm, cuboid: length 40 cm / width 30 cm / high 20 cm / thickness 4 cm) and the matching solid cover wood pulp sponge (cylinder: after absorbing water, diameter 35 cm / thickness 5 cm, cuboid: length 40 cm / width 30 cm / thickness 5 cm) in a sterile environment, and treat with 1L of Pleurotus sajor-caju liquid mycelium per cubic decimeter volume. Distribute the mycelium evenly on the wood pulp sponge, and pour out the excess mycelium.

[0022] Application Evaluation

[0023] The same type of oyster mushroom biodegradable product was prepared according to the matrix molding method provided by Loop Living Cocoon™ as a control (Group A). ​​The application effect was compared with the oyster mushroom biodegradable product obtained with wood pulp sponge as the skeleton (Group B). Two strain control groups were set up (straw mushroom biodegradable product obtained with wood pulp sponge as the skeleton was Group C, and king oyster mushroom biodegradable product obtained with wood pulp sponge as the skeleton was Group D) and a blank group (Group E). The pet used in this study was a pigeon weighing about 600 grams that was slaughtered on the same day. It was only slaughtered by cutting its throat and bleeding it out, without destroying its internal organs. After slaughter, the pigeon was directly put into the product and covered. It was buried in a high place with a burial depth of about 0.4 meters and covered with soil. The degradation effect was evaluated after 45-50 days. Evaluation indicators: (1) Degradation status: Degradation days / Degradation days; (2) Heavy metal residue status: Heavy metal residue in the degraded part / Heavy metal residue in the degraded part / Heavy metal residue in the soil.

[0024] Degradation status: Results showed that after approximately 45 days of degradation, the mycelial blocks in groups A and B were completely degraded, as was the pigeon muscle, leaving only a few coarse feathers and two leg bones. The animal remains exhibited a honeycomb-like, porous, and loose structure, without resembling the original animal, odorless, and completely dry with no exudate. Earthworms in the surrounding environment were significantly more numerous and larger, and peanut seedlings in the vicinity grew better than in other areas. In groups C and D, the pigeon muscle was completely degraded, leaving only a few coarse feathers and two leg bones. The remains resembled the original animal but had an odor. In group E, the pigeon muscle was completely degraded, leaving only a few coarse feathers and two leg bones. The remains resembled the original animal but had an odor.

[0025] Heavy metal residue status: Before degradation, the Cu content in pigeon muscle was higher than 1 ppm, and the Cu and Cd content in the soil was higher than 2 ppm. After approximately 45 days of degradation in groups A and B, the Cu content in mycelial residue and pigeon remains decreased to below 1 ppm, and the Cu and Cd content in the soil decreased to below 1 ppm. After approximately 45 days of degradation in group C, the Cu content in mycelial residue and pigeon remains decreased to below 1 ppm, and the Cu content in the soil decreased to below 1 ppm, while the Cd content in the soil was higher than 2 ppm. After approximately 45 days of degradation in group D, the Cu content in mycelial residue and pigeon remains was higher than 1 ppm, and the Cu content in the soil was higher than 1 ppm, while the Cd content in the soil decreased to below 1 ppm. In group E, the heavy metal (Cu and Cd) content in pigeons and soil remained unchanged before and after degradation.

[0026] Loop Living Cocoon™ products, along with similar large fungal products, show great promise in the degradation of pet carcasses. They offer advantages such as ideal degradation time, high public acceptance, and minimal odor, while also effectively improving surrounding soil and the environment, promoting a healthy balance between flora and fauna. In particular, the lung-shaped plesio ear degradation product, with its wood pulp sponge framework, offers a price advantage, shorter production cycle, and eliminates the need for expensive custom molding equipment, reducing the contamination rate during cultivation and demonstrating broad application prospects.

Claims

1. The application of Pleurotus sajor-caju MT in biotransformation and degradation, characterized in that, The accession number of the aforementioned Pleurotus sajor-caju MT is CCTCC M 2021011.

2. The application according to claim 1, characterized in that, The biotransformation and degradation refers to the degradation of the corpse.

3. The application according to claim 2, characterized in that, The biotransformation and degradation refers to the degradation of animal carcasses.

4. The application according to claim 3, characterized in that, The biotransformation and degradation refers to the degradation of pet carcasses.

5. The application according to claim 2, characterized in that, The specific method of application is as follows: cover the corpse with Pleurotus sajor-caju MT.

6. The application according to claim 5, characterized in that, The specific method for the application is as follows: (1) Preparation of Pleurotus sajor-caju MT mycelium: First, prepare a liquid culture medium: 200-220g peeled potatoes, 8-10g peptone, 20-22g glucose, and add water to 1L; then, inoculate Pleurotus sajor-caju MT into the liquid culture medium and vortex for 20-30 minutes, then culture at 24-26℃ and 160-200r / min for 1-2 days, then let it stand at the same temperature for 2-4 hours, and then culture at 24-26℃ and 60-100r / min for 4-5 days to obtain Pleurotus sajor-caju MT; (2) The mycelium of Pleurotus sajor-caju MT was covered on the surface of the wood pulp sponge, and then the corpse was wrapped in the wood pulp sponge for degradation.

7. The application according to claim 1, characterized in that, The biotransformation and degradation are aimed at reducing the Cu and Cd content in the remains and soil during the degradation of the carcass.