Real-time cell flow rate-based droplet delay adjustment method and system for flow sorters

By dynamically adjusting the droplet delay time and breakpoint position through real-time monitoring of cell flow rate, the problem of poor sorting effect caused by fixed droplet delay time is solved, the purity and yield of sorting are improved, the operation process is simplified, and the cost is reduced.

CN121720908BActive Publication Date: 2026-06-05SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Filing Date
2026-02-26
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

In existing technologies, the droplet delay time is fixed and cannot adapt to changes in the environment or individual factors in real time, resulting in a decrease in cell sorting yield and purity, especially when loading samples at high speed.

Method used

By monitoring cell flow rate in real time and dynamically adjusting droplet delay time, combined with automatic stabilization control of droplet breakpoint position, the stability and accuracy of droplet delay are ensured. A flow cytometer droplet delay adjustment method and system based on real-time cell flow rate is adopted.

Benefits of technology

It improves the purity and yield of cell sorting, simplifies the operation process, reduces experimental costs, and is suitable for cell sorting needs in a variety of biomedical fields.

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Abstract

The application provides a flow cytometer droplet delay adjustment method and system based on real-time cell flow rate, relates to the field of flow sorting, and ensures accurate control of the time of loading charges to droplets in the sorting process by monitoring the cell flow rate in real time and dynamically adjusting the droplet delay time, even if factors such as temperature, nozzle state, air pressure, pipeline and the like change. Meanwhile, real-time dynamic adjustment of the droplet delay time can effectively cope with the random distribution of the cell flow rate when high-speed loading, and ensure that cells at different positions can be accurately sorted. The application realizes automatic calibration and self-stabilization functions, does not require a large number of experimental supplies and a complex optimization process, and only needs to set the initial value of the droplet delay on the basis of real-time monitoring and adjustment, so that accurate control of the droplet delay can be achieved. The application not only improves the sorting efficiency, but also reduces the experimental cost and operation difficulty, and is suitable for cell sorting requirements in various biomedical fields.
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