Scalp lotion with moisturizing, soothing and tightening effects as well as preparation method and application of scalp lotion

Abstract

The invention relates to the field of scalp water, in particular to scalp water with moisturizing, soothing and tightening effects as well as a preparation method and application of the scalp water. According to the scalp lotion, the active ingredients such as the perigord truffle extract, the mistletoe extract, the artemisia capillaris extract and the denseflower rodendranthus herb extract are selected, and the raw materials such as the humectant, the tripeptide-1, the witch hazel extract, the dipotassium glycyrrhizinate and the bisabolol with the moisturizing, relieving and tightening effects are selected, so that the scalp lotion has the moisturizing, relieving and tightening properties; meanwhile, selection of the raw materials is optimized, the process and matching of the extracts are improved, and the performance of tightening the scalp, relieving the scalp and the like is further improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of scalp water, in particular to a scalp water with moisturizing, soothing and firming effects, a preparation method and application thereof. BACKGROUND

[0002] Scalp water is a cosmetic product for the care of scalp skin. It can target multiple aspects to condition the scalp to a healthier and stronger state. Moisturizing is the basic function of such products, which replenishes water to dry scalp, improves tightness and dandruff caused by water deficiency, restores the natural moisture balance of the scalp, and lays the foundation for subsequent care. Soothing focuses on soothing sensitivity and discomfort, which can effectively reduce redness, itching and burning sensation of the scalp caused by environmental irritation, improper cleaning or internal stress, and improve the comfort and tolerance of the scalp. Firming is a higher level of care goal, aiming to improve the elasticity and support of the scalp skin, strengthen the scalp structure, help to improve the weakening of hair support caused by relaxation, and make the overall hair root more stable.

[0003] Invention patent CN121177182A discloses a water solution for scalp care and a preparation method thereof. The solution is prepared by mixing a plurality of raw materials in sequence, wherein the plurality of raw materials include, by concentration parts: mixed cytokine 0.005%; vitamin B12 0.00006%; allantoin 0.2%; nicotinamide 0.7%; chamomile extract 5%; aloe extract 5%; ginkgo extract 5%; ganoderma extract 5%; polyol preservative 1%; and water 100%.

[0004] Invention patent CN121177162A discloses a composition for regulating scalp microecology and its application. The composition for regulating scalp microecology comprises the following components: emu oil, alternanthera philoxeroides leaf oil, Brazilian green propolis and hydrogenated castor oil.

[0005] Invention patent CN121370680A discloses a composite acid gel for caring for the scalp and a preparation method thereof. The gel is prepared by combining salicylic acid, glycolic acid, piroctone olamine, Echinacea-pediococcus fermentum, emulsifier and water, wherein the Echinacea-pediococcus fermentum is prepared by enzymatic hydrolysis of Echinacea with Bacillus subtilis protease and then aerobic fermentation with lactic acid bacteria. The combination of salicylic acid, glycolic acid and azelaic acid in the composite acid gel weakens the irritation of salicylic acid, but retains its effect of dissolving sebum in hair follicles; in addition, glycolic acid and azelaic acid accelerate keratin metabolism and regulate sebum secretion, creating a safe and healthy shield for the scalp environment, and the polysaccharides and flavonoids in Echinacea-pediococcus fermentum are beneficial to its antioxidant and immunomodulatory effects.

[0006] The prior art is less related to the effect of further improving the tightness of the scalp on the basis of improving moisturizing and soothing. Therefore, it is a technical problem to provide a scalp lotion with high-efficiency moisturizing, soothing and tightening effects. SUMMARY

[0007] The present application provides a scalp lotion with moisturizing, soothing and tightening effects, a preparation method and application thereof.

[0008] The present application provides a cosmetic composition, which comprises, by weight, 0.1-2 parts of black truffle extract, 0.1-2 parts of ginkgo kadsura extract, 0.2-2.5 parts of artemisia extract and 0.2-2.5 parts of yellow flower nine-wheel grass extract.

[0009] As a preferred embodiment, the weight ratio of the black truffle extract and the yellow flower nine-wheel grass extract is 1:0.3-2.5, preferably 1:0.8-1.3.

[0010] As a preferred embodiment, the black truffle extract and the yellow flower nine-wheel grass extract are obtained by extraction with 10-25 wt% ethanol solution, filtration, concentration and drying.

[0011] As a preferred embodiment, the preparation method of the black truffle extract comprises: taking dried black truffle, crushing, adding 10-40 times the weight of 10-25 wt% ethanol solution, heating and stirring at 50-65°C for 3-8 hours for extraction, cooling, filtering, concentrating and drying.

[0012] As a preferred embodiment, the preparation method of the yellow flower nine-wheel grass extract comprises: taking dried yellow flower nine-wheel grass, crushing, adding 12-45 times the weight of 10-25 wt% ethanol solution, heating and stirring at 52-65°C for 2-7.5 hours for extraction, cooling, filtering, concentrating and drying.

[0013] As a preferred embodiment, the artemisia extract is obtained by extraction with 60-80 wt% ethanol solution, filtration, concentration and drying.

[0014] As a preferred embodiment, the preparation method of the artemisia extract comprises: taking dried artemisia, crushing, adding 12-50 times the weight of 60-80 wt% ethanol solution, heating and stirring at 50-62°C for 1.5-6 hours for extraction, cooling, filtering, concentrating and drying.

[0015] As a preferred embodiment, the preparation method of the ginkgo kadsura extract comprises: extraction with 65-80 wt% ethanol solution, concentration of the extraction solution, first extraction with ethyl acetate, second extraction with n-butanol, and mixing of the two extracts after concentration under reduced pressure.

[0016] As a preferred aspect, the preparation method of the Ginkgo-Gall extract includes: using 65-80wt% ethanol solution for extraction, concentrating the obtained extraction solution, adding ethyl acetate for first extraction, then separating the layers to obtain ethyl acetate extraction phase and water phase after the first extraction, concentrating the ethyl acetate extraction phase to remove ethyl acetate to obtain product one, adding n-butanol to the water phase after the first extraction for second extraction, then separating the layers to obtain n-butanol extraction phase and water phase after the second extraction, concentrating the n-butanol extraction phase under reduced pressure to remove n-butanol to obtain product two, mixing product one and product two to obtain the Ginkgo-Gall extract.

[0017] As a preferred aspect, the preparation method of the Ginkgo-Gall extract includes: crushing the dried Ginkgo-Gall, adding 10-50 times of 65-80wt% ethanol solution by weight, heating and stirring for extraction at 50-60℃ for 1-6 hours, filtering to obtain the extraction solution, concentrating to obtain the concentrate, adding 1.5-3 times of ethyl acetate by weight of the concentrate, stirring for 1-3 hours for first extraction, then separating the layers to obtain ethyl acetate extraction phase and water phase after the first extraction, concentrating the ethyl acetate extraction phase under reduced pressure to remove ethyl acetate to obtain product one, adding 1.5-3 times of n-butanol by weight of the water phase after the first extraction, stirring for 1-3 hours for second extraction, then separating the layers to obtain n-butanol extraction phase and water phase after the second extraction, concentrating the n-butanol extraction phase under reduced pressure to remove n-butanol to obtain product two, mixing product one and product two to obtain the Ginkgo-Gall extract.

[0018] The application also provides a preparation method of the cosmetic composition, which includes mixing the raw materials and sterilizing.

[0019] The application also provides a scalp water with moisturizing, soothing and tightening effects, which comprises, by weight percentage, 0.1-10% of the composition, 3-6% of a moisturizing agent, 1-4% of a lipid supplement, 0.01-0.1% of tripeptide-1, 0.1-0.8% of hamamelis virginiana extract, 0.01-0.1% of dipotassium glycyrrhizate, 0.01-0.2% of bisabolol, 0.3-1.5% of an emulsifier, 0.08-0.5% of a preservative, 0.1-0.8% of a pH adjuster and the balance of water.

[0020] As a preferred aspect, the moisturizing agent includes at least one of panthenol, sodium hyaluronate, glycerol, sodium PCA, propylene glycol, butylene glycol and ethylene glycol.

[0021] As a preferred aspect, the lipid supplement includes at least one of squalane, ceramide, grape seed oil and caprylic / capric triglyceride.

[0022] As a preferred embodiment, the emulsifier comprises at least one of Tween-60, polyglyceryl-3 methylglucose distearate, cetyl stearyl glucoside.

[0023] As a preferred embodiment, the pH regulator comprises at least one of citric acid, sodium citrate.

[0024] The present application also provides a preparation method of the scalp lotion, comprising the following steps: (1) heating water, adding a humectant and an emulsifier, stirring and mixing to obtain a mixture; (2) adding a lipid supplement, dipotassium glycyrrhizinate and farnesol to the mixture, stirring and mixing to obtain a mixed solution; (3) cooling the mixed solution, adding the cosmetic composition, tripeptide-1, hamamelis extract, a preservative and a pH regulator, stirring and mixing, standing, sterilizing to obtain the scalp lotion.

[0025] As a preferred embodiment, the water is heated to 50-65℃ in step (1).

[0026] As a preferred embodiment, the stirring speed is 100-300 rpm in step (1).

[0027] As a preferred embodiment, the stirring speed is 500-2000 rpm in step (2).

[0028] As a preferred embodiment, the stirring speed is 400-800 rpm in step (3).

[0029] In addition, the above-mentioned scalp lotion can also selectively add plant extracts, peptides and other active ingredients according to other functional requirements, for example but not limited to: one or more of Ganoderma atrum extract, Vitis vinifera seed oil, Macadamia integrifolia seed oil, mycosis peptide, tripeptide-1 copper, fructooligosaccharide, Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, Camellia sinensis leaf extract, Glycyrrhiza glabra root extract, Anthemis nobilis flower extract, Rosmarinus officinalis leaf extract.

[0030] The present application has the following advantages: The black truffle extract and the yellow flower nine round grass extract obtained by the 10-25wt% ethanol solution leaching process can effectively inhibit elastase, and have excellent firming scalp performance. Through comparison, it is found that: when the weight ratio of the black truffle extract and the yellow flower nine round grass extract is 1:0.1, 1:5.5, the simple superposition of the two can improve the elastase inhibition effect; when the weight ratio is in the range of 1:0.3-2.5, the elastase inhibition effect of the combination is better than that of single effect, and a synergistic effect is presented; especially when the weight ratio is in the range of 1:0.8-1.3, the elastase inhibition effect of the combination is the best compared with other ratios.

[0031] The white ginkgo mistletoe extract obtained by the 65-80wt% ethanol solution leaching + ethyl acetate first extraction + n-butanol second extraction process can effectively inhibit the activity of hyaluronidase and effectively relieve the skin. The present application studies that: the white ginkgo mistletoe extract obtained by low-concentration ethanol extraction has low relieving effect, the extract obtained by 65-80wt% ethanol leaching has excellent relieving performance. And the method of first ethyl acetate extraction and then n-butanol extraction can improve the relieving performance of the extract to a certain extent; and the method of first ethyl acetate extraction and then n-butanol extraction can significantly improve the relieving performance of the white ginkgo mistletoe extract.

[0032] The composition containing the black truffle extract, the white ginkgo mistletoe extract, the wormwood extract and the yellow flower nine round grass extract, and the moisturizing agent, the tripeptide-1, the hamamelis extract, the dipotassium glycyrrhizate, the bisabolol and other raw materials with moisturizing, soothing and firming effects, so that the scalp water has moisturizing, soothing and firming performance; at the same time, the present application optimizes the selection of raw materials and improves the black truffle extract, the yellow flower nine round grass extract and the white ginkgo mistletoe extract, further improves the performance of the raw materials in firming the scalp and soothing the scalp, and further improves the performance of the scalp water. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 : The elastase inhibition rate of each group.

[0034] Figure 2 : The hyaluronidase inhibition rate of each group. DETAILED DESCRIPTION

[0035] The present application will be further illustrated below in conjunction with the embodiments and the drawings. It should be understood that these embodiments are only used to illustrate the present application, and are not used to limit the scope of the present application.

[0036] Example One Black truffle extract: dry black truffle (Tuber sinense) was crushed and sieved to 50 mesh, 25 times the weight of the powder of 20wt% ethanol solution was added, and the mixture was heated and stirred at 55°C and 120 rpm for 4.5 hours. After cooling to room temperature, the mixture was filtered, and the filtrate was concentrated at 55°C under reduced pressure to a solid content of 19.3wt%. Then, the mixture was freeze-dried at -45°C to a water content of 1.97wt%.

[0037] Primula veris extract: dry primula veris (Primula veris) was crushed and sieved to 50 mesh, 30 times the weight of the powder of 22.5wt% ethanol solution was added, and the mixture was heated and stirred at 57.5°C and 100 rpm for 3.5 hours. After cooling to room temperature, the mixture was filtered, and the filtrate was concentrated at 55°C under reduced pressure to a solid content of 21.78wt%. Then, the mixture was freeze-dried at -50°C to a water content of 1.83wt%.

[0038] The above extracts and the combination thereof were tested for their skin tightening effects, and the inhibition rate of elastase was tested as the detection index.

[0039] Test reagents and solutions used: Composition of samples: Sample I: black truffle extract; Sample II: primula veris extract; Sample III: black truffle extract and primula veris extract in a weight ratio of 1:0.1; Sample IV: black truffle extract and primula veris extract in a weight ratio of 1:0.3; Sample V: black truffle extract and primula veris extract in a weight ratio of 1:0.8; Sample VI: black truffle extract and primula veris extract in a weight ratio of 1:1; Sample VII: black truffle extract and primula veris extract in a weight ratio of 1:1.3; Sample VIII: black truffle extract and primula veris extract in a weight ratio of 1:2.5; Sample IX: black truffle extract and primula veris extract in a weight ratio of 1:5.5.

[0040] 0.1M, pH8 TRIS-HCl buffer (referred to as buffer); tea polyphenol solution (0.9wt%); substrate solution (2mM N-succinyl-alanine-alanine-alanine-p-nitroaniline solution); elastase solution (0.175U / mL porcine pancreatic elastase solution); the solvents used for tea polyphenol solution, substrate solution and elastase solution were buffer; sample solution (0.8wt% solution of samples 1-9, solvent was buffer containing 0.5wt% Tween-60).

[0041] Group setting: Model group: 25 μL elastase solution + 50 μL substrate solution + 25 μL buffer solution; Blank group: 50 μL substrate solution + 50 μL buffer solution; Positive group: 25 μL tea polyphenol solution + 25 μL elastase solution + 50 μL substrate solution; Sample I-IX group: 25 μL sample solution + 25 μL elastase solution + 50 μL substrate solution.

[0042] Each group of solutions is prepared according to the above, and after being placed at room temperature in the dark for 20 min, the absorbance is tested at 420 nm by using an enzyme label instrument, the inhibition rate is calculated according to the absorbance, and each group is tested in triplicate to take an average value.

[0043] The elastase inhibition rate calculation formula is 100% x [1- (OD1-OD0) / (OD2-OD0)]. OD0: absorbance of the blank group; OD1: absorbance of the sample group; OD2: absorbance of the model group.

[0044] The elastase inhibition rate obtained by testing and the proportion K value of the inhibition rate improvement compared with the sample II are calculated according to each group, and the test results are shown in Table 1 and Figure 1 .

[0045] Table 1: Elastase inhibition rate Group Elastase inhibition rate K value Positive group 60.34% / Sample I 43.15% 9.05% Sample II 39.57% / Sample III 42.05% 6.27% Sample IV 45.62% 15.29% Sample V 52.24% 32.02% Sample VI 54.83% 38.56% Sample VII 50.79% 28.35% Sample VIII 44.68% 12.91% Sample IX 41.31% 4.40% According to the test results in Table 1 and Figure 1 It can be obtained that the black truffle extract and the yellow flower nine round grass extract obtained by the 65-80 wt% ethanol solution extraction process can effectively inhibit elastase, and have excellent firming scalp performance. Samples III-IX are combinations of the two, and it is found through comparison that: when the black truffle extract and the yellow flower nine round grass extract are in a weight ratio of 1:0.1, 1:5.5, the effect shows a simple superposition relationship; when the weight ratio is in the range of 1:0.3-2.5, the combination produces an elastase inhibition effect better than the single effect, showing a synergistic effect, especially when the weight ratio is in the range of 1:0.8-1.3, the combination produces the best effect of improving the elastase inhibition.

[0046] Example Two Ginkgo biloba mistletoe extract A: Dried ginkgo biloba mistletoe was pulverized and passed through a 50-mesh sieve to obtain pulverized material. A 75wt% ethanol solution, 20 times the weight of the pulverized material, was added. The mixture was heated and stirred at 57°C and 100 rpm for 3 hours. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3 times the weight of the pulverized material. A concentrate was obtained by adding ethyl acetate, 2 times the weight of the concentrate, and stirring at 45°C and 80 rpm for 2 hours for the first extraction. The mixture was then allowed to stand at room temperature for 1 hour to separate the layers, yielding an ethyl acetate extract and an aqueous phase after the first extraction. The ethyl acetate extract was concentrated under reduced pressure at 50°C to remove the ethyl acetate, yielding product one. A second extraction was performed by adding n-butanol, 2 times the weight of the aqueous phase, to the aqueous phase after the first extraction. The mixture was stirred at 50°C and 80 rpm for 2 hours, yielding a second extraction. The mixture was then allowed to stand at room temperature for 1.5 hours to separate the layers, yielding an n-butanol extract and an aqueous phase after the second extraction. The n-butanol extract was concentrated under reduced pressure at 65°C to remove the n-butanol, yielding product two. Product one and product two were mixed to obtain ginkgo biloba mistletoe extract.

[0047] Ginkgo biloba mistletoe extract B: Dried ginkgo biloba mistletoe was pulverized and passed through a 50-mesh sieve to obtain a powder. A 65wt% ethanol solution (25 times the weight of the powder) was added, and the mixture was heated and stirred at 55°C and 100 rpm for 2.8 hours for extraction. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3.2 times the weight of the powder. A concentrate (2.3 times the weight of the concentrate) was added, and the mixture was stirred at 40°C and 80 rpm for 2.2 hours for the first extraction. The mixture was then allowed to stand at room temperature for 1.2 hours to separate the layers, yielding the ethyl acetate extract. The ethyl acetate extract was concentrated under reduced pressure at 45°C to remove ethyl acetate, yielding product one. Two to three times the weight of n-butanol was added to the aqueous phase after the first extraction, and the mixture was stirred at 55°C and 80 rpm for 1.8 hours for a second extraction. The mixture was then allowed to stand at room temperature for 1.5 hours to separate the layers, yielding the n-butanol extract and the aqueous phase after the second extraction. The n-butanol extract was concentrated under reduced pressure at 67°C to remove n-butanol, yielding product two. Product one and product two were mixed to obtain the *Mistletoe ginseng* extract.

[0048] Ginkgo biloba mistletoe extract C: Compared to extract A, it uses low-concentration ethanol extraction. The specific process is as follows: Dried *Mistletoe* sp. was pulverized and passed through a 50-mesh sieve to obtain a powder. A 30wt% ethanol solution, 20 times the weight of the powder, was added. The mixture was heated and stirred at 57°C and 100 rpm for 3 hours to extract the powder. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3 times the weight of the powder. A concentrate was obtained by adding ethyl acetate, 2 times the weight of the concentrate, and stirring at 45°C and 80 rpm for 2 hours for the first extraction. The mixture was then allowed to stand at room temperature for 1 hour to separate the layers, yielding an ethyl acetate extract and an aqueous phase after the first extraction. The ethyl acetate extract was concentrated under reduced pressure at 50°C to remove the ethyl acetate, yielding product one. A second extraction was performed by adding n-butanol, 2 times the weight of the aqueous phase, and stirring at 50°C and 80 rpm for 2 hours. The mixture was then allowed to stand at room temperature for 1.5 hours to separate the layers, yielding an n-butanol extract and an aqueous phase after the second extraction. The n-butanol extract was concentrated under reduced pressure at 65°C to remove the n-butanol, yielding product two. Product one and product two were mixed to obtain *Mistletoe* sp. extract.

[0049] Ginkgo biloba mistletoe extract D: Compared to extract A, the extraction order is different; it is first extracted with n-butanol, then with ethyl acetate. The specific process is as follows: Dried *Mistletoe* sp. was pulverized and passed through a 50-mesh sieve to obtain a powder. A 75wt% ethanol solution, 20 times the weight of the powder, was added. The mixture was heated and stirred at 57°C and 100 rpm for 3 hours to extract the powder. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3 times the weight of the powder. A concentrate was obtained by adding n-butanol, 2 times the weight of the concentrate, and stirring at 50°C and 80 rpm for 2 hours for the first extraction. The mixture was then allowed to stand at room temperature for 1.5 hours to separate the layers, yielding the n-butanol extract and the aqueous phase after the first extraction. The n-butanol extract was concentrated under reduced pressure at 65°C to remove the n-butanol, yielding product one. A second extraction was performed by adding ethyl acetate, 2 times the weight of the aqueous phase after the first extraction, and stirring at 45°C and 80 rpm for 2 hours. The mixture was then allowed to stand at room temperature for 1 hour to separate the layers, yielding the ethyl acetate extract and the aqueous phase after the second extraction. The ethyl acetate extract was concentrated under reduced pressure at 50°C to remove the ethyl acetate, yielding product two. Product one and product two were mixed to obtain the *Mistletoe* sp. extract.

[0050] Ginkgo biloba mistletoe extract E: Compared to extract A, it does not undergo ethyl acetate extraction. The specific process is as follows: Dried mistletoe was pulverized and passed through a 50-mesh sieve to obtain a powder. A 75wt% ethanol solution, 20 times the weight of the powder, was added and the mixture was heated and stirred at 57°C and 100 rpm for 3 hours. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3 times the weight of the powder. A concentrate was obtained and 2 times the weight of the concentrate was added to n-butanol. The mixture was stirred at 50°C and 80 rpm for 2 hours for extraction. The mixture was then allowed to stand at room temperature for 1.5 hours to separate the layers, yielding the n-butanol extract phase and the aqueous phase after extraction. The n-butanol extract phase was concentrated under reduced pressure at 65°C to remove the n-butanol, yielding the mistletoe extract.

[0051] Ginkgo biloba mistletoe extract F: Compared to extract A, it does not undergo n-butanol extraction. The specific process is as follows: Dried mistletoe was pulverized and passed through a 50-mesh sieve to obtain pulverized material. A 75wt% ethanol solution, 20 times the weight of the pulverized material, was added. The mixture was heated and stirred at 57°C and 100 rpm for 3 hours to extract the pulverized material. The extract was filtered and concentrated under reduced pressure at 55°C until the concentrate was 3 times the weight of the pulverized material. A concentrate was obtained by adding ethyl acetate, 2 times the weight of the concentrate, and stirring at 45°C and 80 rpm for 2 hours for the first extraction. The mixture was then allowed to stand at room temperature for 1 hour to separate the layers, yielding an ethyl acetate extract and an aqueous phase after the first extraction. The ethyl acetate extract was concentrated under reduced pressure at 50°C to remove the ethyl acetate, yielding the mistletoe extract.

[0052] The skin-soothing effects of the above extracts were tested, with the inhibition rate of hyaluronidase as the detection indicator.

[0053] Reagents and solutions used in the test: Hyaluronidase (450 U / mg); Acetate buffer (acetic acid / sodium acetate, pH=5.6); Ehrlich reagent (1.6 g p-dimethylaminobenzaldehyde + 30 mL concentrated hydrochloric acid + 30 mL ethanol); Hyaluronidase solution (2.5 mg / mL, with acetate buffer as solvent); Sodium hyaluronate solution (0.5 mg / mL, with acetate buffer as solvent); Acetylacetone solution (3.5 mL acetylacetone + 50 mL 1.0 mol / L sodium carbonate solution).

[0054] Test procedure: Mix 0.1 mL of calcium chloride solution (0.25 mol / L) with 0.5 mL of hyaluronidase solution, and let stand at 37°C in the dark for 30 minutes. Add 0.5 mL of extract solution (dilute Ginkgo biloba extract with deionized water to a concentration of 0.1 wt%), and let stand at 37°C in the dark for 30 minutes. Then add 0.5 mL of sodium hyaluronate solution, and let stand at 37°C in the dark for 30 minutes. Allow to cool naturally to room temperature. Add 0.1 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of acetylacetone solution. Incubate in a boiling water bath for 15 minutes, then in an ice bath for 6 minutes. Add 1 mL of Ehrlich reagent and 3 mL of ethanol, mix, and let stand at room temperature for 30 minutes for color development. Then use a spectrophotometer to measure the absorbance at a wavelength of 528 nm. Calculate the hyaluronidase inhibition rate based on the absorbance. Perform three parallel tests for each group.

[0055] The formula for calculating the hyaluronidase inhibition rate is 100% × [(OD200%)]. A -OD B )-(OD C -OD D )] / (OD A -OD B ); where OD C The absorbance values ​​for the extracted solution group were measured, OD. A These are the absorbance values ​​of the groups obtained after replacing the extraction solution with acetate buffer; OD B These are the absorbance values ​​of the groups obtained after replacing the extraction solution and hyaluronidase solution with acetate buffer; OD D The absorbance values ​​are the values ​​of the groups obtained after replacing the hyaluronidase solution with acetate buffer.

[0056] The inhibition rate data obtained from the test and the reduction ratio Q value of the inhibition rate compared with extract A are shown in Table 2 and 2. Figure 2 .

[0057] Table 2: Hyaluronidase inhibition rate and Q value Group Hyaluronidase inhibition rate Q value Viscum album extract A 64.35% / Viscum album extract B 62.03% 3.61% Viscum album extract C 41.79% 35.06% Viscum album extract D 57.82% 10.15% Viscum album extract E 50.24% 21.93% Viscum album extract F 55.16% 14.28% Combine Table 2 and Figure 2Test results show that the *Mistletoe* extract obtained by the present invention, using a 65-80 wt% ethanol solution extraction followed by a first ethyl acetate extraction and a second n-butanol extraction, effectively inhibits hyaluronidase activity and soothes the skin. A comparison of extracts A and C shows that the *Mistletoe* extract obtained from low-concentration ethanol extraction has a lower soothing effect, while the extract obtained by the present invention using 65-80 wt% ethanol extraction exhibits excellent soothing properties. A comparison of extracts A, D, E, and F shows that using ethyl acetate followed by n-butanol extraction can improve the soothing properties of the extract to some extent; furthermore, the sequential extraction of ethyl acetate and n-butanol in this invention significantly enhances the soothing properties of the *Mistletoe* extract.

[0058] Example 3 Preparation of a scalp lotion containing black truffle extract, ginkgo mistletoe extract, artemisia capillaris extract and scalp styrax extract.

[0059] Artemisia capillaris extract: Take dried Artemisia capillaris (aerial part), pulverize and pass through 50 mesh, add 20 times its weight of 70wt% ethanol solution, heat and stir at 55℃ and 100rpm for 3.5 hours for extraction, cool to room temperature, filter, concentrate under reduced pressure at 55℃ to a solid content of 24.3wt%, freeze dry at -50℃ to a water content of 1.95wt%, and obtain.

[0060] Witch hazel extract: Take dried witch hazel leaves, crush them, pass them through a 50-mesh sieve, add 30 times their weight of water, heat and stir at 85℃ and 100rpm for 3.5 hours for extraction, cool to room temperature, filter, concentrate under reduced pressure at 60℃ to a solid content of 20.3wt%, freeze dry at -54℃ to a water content of 2.76wt%, and obtain the extract.

[0061] Scalp lotion 1: Raw material composition, wt%: 5.5% composition, 5% moisturizer, 3% lipid supplement; 0.08% tripeptide-1, 0.3% witch hazel extract, 0.05% dipotassium glycyrrhizate, 0.04% bisabolol, 1.2% emulsifier, 0.3% preservative phenoxyethanol, 0.7% pH adjuster citric acid and balance water; The composition comprises, by weight, 1.2 parts black truffle extract, 1.5 parts mistletoe extract, 0.5 parts artemisia capillaris extract, and 1.2 parts senna extract; the composition is obtained by mixing and sterilizing the above extracts. Moisturizer: Panthenol, sodium hyaluronate, and glycerin in a weight ratio of 0.1:0.2:1; Lipid supplement: composed of squalane, ceramide, and grape seed oil in a weight ratio of 1:1:3; Emulsifier: Tween-60 and polyglycerol-3-methylglucose distearate in a weight ratio of 1:0.4.

[0062] The preparation method is as follows: (1) Heat water to 60°C, add humectant and emulsifier, stir and mix at 200 rpm for 20 minutes to obtain a mixture; (2) Add lipid supplement, dipotassium glycyrrhizate and bisabolol to the mixture, stir at 1500 rpm for 30 minutes to obtain a mixture; (3) Cool the mixture to 40°C, add the cosmetic composition, tripeptide-1, witch hazel extract, preservative and pH adjuster, stir and mix at 500 rpm for 30 minutes, let stand, sterilize, and obtain the scalp water.

[0063] Scalp lotion 2: Raw material composition, wt%: 1.5% composition, 4.5% moisturizer, 1.5% lipid supplement; 0.07% tripeptide-1, 0.25% witch hazel extract, 0.04% dipotassium glycyrrhizate, 0.03% bisabolol, 1.05% emulsifier, 0.4% preservative phenoxyethanol, 0.8% pH adjuster citric acid, and balance water; The composition comprises, by weight, 1.5 parts black truffle extract, 1.1 parts mistletoe extract, 0.4 parts Artemisia capillaris extract, and 1.6 parts Hedyotis diffusa extract; the composition is obtained by mixing the above extracts. Moisturizer: Panthenol, sodium hyaluronate, and glycerin in a weight ratio of 0.1:0.5:1; Lipid supplement: composed of squalane, ceramide, and grape seed oil in a weight ratio of 1:1.2:3; Emulsifier: Tween-60 and polyglycerol-3-methylglucose distearate in a weight ratio of 1:0.7.

[0064] The preparation method of scalp lotion 2 is the same as that of scalp lotion 1.

[0065] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A cosmetic composition, characterized by comprising: The raw material composition comprises, by weight parts: 0.1-2 parts of black truffle extract, 0.1-2 parts of ginkgo kurchi extract, 0.2-2.5 parts of wormwood extract, and 0.2-2.5 parts of yellow flower nine-wheel grass extract.

2. The cosmetic composition according to claim 1, characterized by The weight ratio of the black truffle extract and the yellow flower nine-wheel grass extract is 1:0.3-2.5, preferably 1:0.8-1.

3.

3. A method for producing the cosmetic composition according to any one of claims 1 to 2, characterized by, The method comprises mixing the raw materials and sterilizing.

4. A scalp water having moisturizing, soothing, and firming efficacy, characterized in that, The raw material composition comprises, by weight percentage: 0.1-10% of the composition of any one of claims 1-2, 3-6% of a humectant, 1-4% of a lipid supplement, 0.01-0.1% of tripeptide-1, 0.1-0.8% of hamamelis extract, 0.01-0.1% of dipotassium glycyrrhizinate, 0.01-0.2% of bisabolol, 0.3-1.5% of an emulsifier, 0.08-0.5% of a preservative, 0.1-0.8% of a pH adjuster, and the balance of water.

5. The scalp water according to claim 4, characterized by, The humectant comprises at least one of panthenol, sodium hyaluronate, glycerol, sodium PCA, propylene glycol, butylene glycol, and ethylene glycol.

6. The scalp water according to claim 4, characterized by, The lipid supplement comprises at least one of squalane, ceramide, grape seed oil, and caprylic / capric triglyceride.

7. The scalp water according to claim 4, characterized by, The emulsifier comprises at least one of Tween-60, polyglyceryl-3 methylglucose distearate, and cetyl stearyl glucoside.

8. The scalp water according to claim 4, characterized by, The pH adjuster comprises at least one of citric acid and sodium citrate.

9. A method of preparing the scalp water according to any one of claims 4 to 8, characterized in that, The method comprises the following steps: (1) heating water, adding a humectant and an emulsifier, stirring and mixing to obtain a mixture; (2) adding a lipid supplement, dipotassium glycyrrhizinate, and bisabolol to the mixture, stirring and mixing to obtain a mixed solution; (3) cooling the mixed solution, adding the cosmetic composition, tripeptide-1, hamamelis extract, a preservative, and a pH adjuster, stirring and mixing, standing, sterilizing, and obtaining the scalp water.

10. The method of claim 10, wherein, The water is heated to 50-65°C.

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