Method for ecologically and cyclically domesticating high-quality maggot and domesticated population thereof

Abstract

The invention relates to the technical field of medicinal breeding of special insects, in particular to a method for ecologically and cyclically domesticating high-quality maggot and a domesticated population thereof, and the method comprises the following steps: seed source acquisition and identification: acquiring chrysomyia megacephala adults, performing purebred identification and health detection, and pre-screening an initial seed source; hatching eggs of the obtained provenance in an artificial environment, feeding larvae of 1-3 years old, applying primary environmental stress, and screening individuals; putting imagoes into the natural environment after emergence of the individuals, and recovering spawning blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, composite environmental stress is applied, and individuals with tolerance reaching the standard are screened out; after the individuals emerge adults, putting the adults into a complex natural environment, and recovering the spawn blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, ultimate environmental stress is applied, and domesticated populations with solidified characters are obtained through screening. Through three-generation directional domestication, artificial stress, natural energizing and fresh fish waste utilization, the effects of pure maggot variety, strong stress resistance, high activity and stable breeding are achieved.

Description

Technical Field

[0001] This invention relates to the field of medicinal insect breeding technology, specifically to a method for ecologically cyclically domesticating high-quality grain insects and its domesticated population. Background Technology

[0002] The larvae of the golden fly (Bacillus thunbergii) are an important raw material for functional health products such as immunomodulatory proteins and natural antimicrobial peptide extracts, as well as for biological protein feed. Their market application value has driven the gradual development of aquaculture technology. Early aquaculture activities mainly relied on the direct capture and utilization of wild stock. With the continuous expansion of market demand, artificial breeding has gradually replaced traditional wild collection. Some existing technologies have begun to attempt preliminary breeding work by optimizing artificial feed formulations or applying single environmental stresses, attempting to improve aquaculture yield and basic quality. However, the overall technical level remains at a relatively basic exploratory stage. Traditional breeding methods have long been constrained by multiple core issues. They lack a systematic breeding system, rely excessively on wild sources leading to a chaotic genetic background, unstable traits, and significant crossbreeding. The artificial breeding environment is monotonous, and larvae and adults do not undergo targeted environmental adaptation training during their growth cycle, resulting in weak tolerance to adverse environmental stresses such as extreme temperature and humidity and high ammonia concentrations. The neglect of natural nutrient supplementation during breeding deprives adults and larvae of natural energy sources such as pollen and microorganisms, leading to insufficient accumulation of antioxidants like SOD and immune-active substances, making it difficult to meet high-quality requirements. Furthermore, the lack of effective methods for inducing and collecting egg-laying results in dispersed adult egg production and low egg mass recovery efficiency, directly impacting the stability of population expansion and the effectiveness of large-scale breeding. While existing technologies have made attempts at improving artificial feed or treating single environmental stresses, they have failed to achieve an organic integration of artificial breeding and natural nutrient provision, nor have they achieved trait solidification through multi-generational targeted selection, making it difficult to cultivate breeding populations with excellent overall performance. Summary of the Invention

[0003] This invention provides an ecological cycle domestication method that, through three generations of cyclical breeding, combined with artificial extreme environmental pressure and natural energy empowerment, cultivates a population of grain insects with high varietal purity, strong stress resistance, and excellent natural active ingredients, thus solving the pain points of traditional breeding. To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows: A method for ecologically acclimating high-quality grain insects includes the following steps: (1) Seed source acquisition and identification: Adult bighead fly was obtained, purebred identification and health testing were carried out, and individuals with natural vitality were pre-screened as the initial seed source; (2) First generation basic domestication: The eggs of the seed source obtained in step (1) are hatched in an artificial environment and 1-3 instar larvae are raised. Primary environmental stress is applied, and individuals with tolerance are selected. After the individuals emerge as adults, they are released into the natural environment for energy empowerment and reproduction, and the egg masses they produce are collected. (3) Second generation intensive domestication: The egg masses recovered in step (2) are hatched and raised as 1st to 3rd instar larvae. Complex environmental stress is applied, and individuals with the required tolerance are selected. After the individuals emerge as adults, they are released into a complex natural environment for energy empowerment and reproduction, and the egg masses they produce are recovered. (4) Third generation stable domestication: The egg masses recovered in step (3) are hatched and raised as 1st to 3rd instar larvae. Ultimate environmental stress is applied, and the larvae are screened according to the preset mortality rate, developmental synchronicity and egg production standards to obtain a domesticated population with solidified traits. Furthermore, in step (1): Purebred identification includes morphological identification based on adult morphological characteristics and molecular marker identification based on mitochondrial COI gene and microsatellite DNA markers; Health testing includes the detection of pathogenic microorganisms and parasite eggs; Pre-screening for natural viability includes observing adult insects' attraction to pollen and / or testing their flight endurance. Further, in step (2), fresh fish fermented feed is used to induce adult insects to lay eggs in a concentrated manner and to serve as the main food for 1st to 3rd instar larvae; the raw material composition of fresh fish fermented feed by weight percentage is: 60% fresh miscellaneous fish, 20% wheat bran, 10% soybean meal, 5% yeast powder, 3% compound probiotics, 1% salt, 1% trace elements, fermented until pH value ≤ 5.0. Further, in step (2), the initial environmental stress is to apply the following stresses within 60 hours of the development of 1st to 3rd instar larvae: temperature stress of raising the temperature to 35±1℃ or lowering it to 20±1℃ for 2-3 hours daily during the 24th to 36th hour of the development of 3rd instar larvae; humidity stress of lowering the humidity to 40±5% or raising it to 90±5% during the 12th to 24th hour of the development of 2nd instar larvae; and ammonia concentration stress of raising the environmental ammonia concentration to 15-20ppm and continuing for 4-6 hours daily throughout the entire development of 3rd instar larvae. Furthermore, in steps (2) and (3), the time for releasing the animal back into the natural environment to recharge its energy is 7-10 days. Further, in step (3), the combined environmental stress is to apply a combination of environmental stresses for 2-3 days during the development of 1st-3rd instar larvae. The combined environmental stresses include: a first stress combination with a temperature of 38±1℃ and a humidity of 90±5%, or a second stress combination with a temperature of 15±1℃ and a humidity of 30±5%; and an ammonia concentration stress that raises the environmental ammonia concentration to 25-30ppm and exposes the larvae to ammonia concentration for 6-8 hours per day. Further, in step (4), the ultimate environmental stress is to apply temperature stress of 39±1℃ or 10±1℃, humidity stress of 25±5% or 98±2% and ammonia concentration stress of 35-40ppm during the development of 1st-3rd instar larvae; each stress factor is applied for 4-6 hours per day for 3 days. Furthermore, in step (4), the screening criteria are: larval mortality rate ≤5%, developmental synchronicity ≥90%, and adult single-laying egg quantity ≥180 eggs. Furthermore, in steps (2) and (3), the oviposition area for inducing adult insects to lay eggs is a sealed box containing a fish-decay substrate and a fermentation liquid containing sex pheromone analogs. Furthermore, the five-grain insect population was obtained through domestication using the methods described above. The advantages of this invention compared to the prior art are: This invention achieves a genetic similarity of ≥95% for the population after three generations of domestication by implementing dual purebred identification using morphology and molecular markers, combined with multi-stage screening during three generations of targeted domestication and isolation and protection of the breeding environment. This ensures stable core traits and allows for repeated breeding, thus improving the problem of mixed varieties caused by traditional breeding relying on wild sources. This invention applies progressively upgraded environmental stresses, from the first generation of primary environmental stress to the second generation of complex environmental stress to the third generation of ultimate environmental stress, allowing larvae to continuously adapt to extreme temperature and humidity and high ammonia environments during the domestication process. This enables larvae to achieve stable tolerance to high temperatures of 39°C, low temperatures of 10°C, high humidity of 98%, and high ammonia of 40ppm, significantly improving the adaptability of the grain insect in various breeding scenarios. In both generations of domestication, the adult insects are released back into the natural environment, allowing them to freely feed on pollen, nectar, and natural microorganisms, and fully absorb natural energy to complete their empowerment. This model, which combines artificial domestication with natural nourishment, achieves a 30%-40% increase in SOD activity in larvae compared to wild species, significantly enhancing their functional value as a raw material for health products and meeting the market's core demand for highly active raw materials. This invention uses a specially formulated fermented fresh fish feed to induce adult insects to lay eggs in a concentrated manner. Combined with the scientific design of a dedicated egg-laying area, and through multi-generational screening to retain individuals with high reproductive performance, the invention achieves an adult egg-laying recovery rate of ≥90%, significantly improving the concentration of egg-laying, ensuring the stability of population expansion, and effectively solving the prominent problems of scattered egg-laying and difficult recovery in traditional aquaculture. This invention uses fresh fish waste as the main raw material to prepare fermented feed, transforming low-value waste into high-value grain insect products. At the same time, the adult insects released during the domestication process will naturally participate in plant pollination, helping the reproduction and growth of plants in the ecosystem, thus achieving efficient recycling of resources and possessing good ecological benefits. Attached Figure Description The accompanying drawings, which form part of this application, are used to provide a further understanding of the application and to make other features, objects, and advantages of the application more apparent. The illustrative embodiments and descriptions of this application are used to explain the application and do not constitute an undue limitation of the application. In the attached diagram: Figure 1 This is a flowchart of a method for ecologically cycling and domesticating high-quality grain insects in Example 1. Detailed Implementation The following detailed description of the embodiments is used to exemplify the principles of this application, but should not be used to limit the scope of this application. That is, the method of ecological cycle domestication of high-quality grain insects and the domesticated population of this application are not limited to the described embodiments. The present invention will be further described below with reference to embodiments. like Figure 1 As shown, a method for ecologically acclimating high-quality grain insects includes the following steps: (1) Seed source acquisition and identification: Adult bighead fly was obtained, purebred identification and health testing were carried out, and individuals with natural vitality were pre-screened as the initial seed source; (2) First generation basic domestication: The eggs of the seed source obtained in step (1) are hatched in an artificial environment and 1-3 instar larvae are raised. Primary environmental stress is applied, and individuals with tolerance are selected. After the individuals emerge as adults, they are released into the natural environment for energy empowerment and reproduction, and the egg masses they produce are collected. (3) Second generation intensive domestication: The egg masses recovered in step (2) are hatched and raised as 1st to 3rd instar larvae. Complex environmental stress is applied, and individuals with the required tolerance are selected. After the individuals emerge as adults, they are released into a complex natural environment for energy empowerment and reproduction, and the egg masses they produce are recovered. (4) Third generation stable domestication: The egg masses recovered in step (3) are hatched and raised as 1st to 3rd instar larvae. Ultimate environmental stress is applied, and the larvae are screened according to the preset mortality rate, developmental synchronicity and egg production standards to obtain a domesticated population with solidified traits. In a specific embodiment, an unpolluted valley was selected as the seed source collection site, and 500 wild golden fly adults at the emergence stage were collected. The collected adults underwent purebred identification, health testing, and natural vitality pre-screening, ultimately selecting 300 high-quality initial seed sources that met the requirements. The eggs from this seed source were placed in an artificially controlled environment for incubation, successfully yielding 4500 larvae. During the 1st to 3rd instar period, initial environmental stress was applied according to a set plan, and after screening, 2800 individuals with adequate tolerance were obtained. After these individuals emerged as adults, they were released into flower beds for energy empowerment and natural reproduction. Seven days later, the egg masses laid by the adults were collected. The collected egg masses were used as the seed source to hatch larvae, subjected to complex environmental stress, and individuals that met the requirements were selected. After emergence, these larvae were released into complex natural environments such as alpine flower beds and wetland edges to complete energy empowerment and reproduction, after which the egg masses were collected again. The egg mass was hatched to produce 16,000 larvae. Ultimate environmental stress was applied and the larvae were strictly screened according to the selection criteria. Finally, 14,000 larvae that met all the criteria were selected to form a domesticated population with fixed traits and stable inheritance. Furthermore, in step (1): Purebred identification includes morphological identification based on adult morphological characteristics and molecular marker identification based on mitochondrial COI gene and microsatellite DNA markers; Health testing includes the detection of pathogenic microorganisms and parasite eggs; Pre-screening for natural viability includes observing adult insects' attraction to pollen and / or testing their flight endurance. In a specific embodiment, the seed source identification stage strictly adheres to a dual identification standard. Morphological identification is achieved through meticulous observation of the head characteristics and wing vein patterns of the adults. Molecular marker identification employs a dual-marking method combining COI gene barcoding and microsatellite DNA, confirming that all selected individuals are purebred. The health testing stage focuses on detecting the presence of Salmonella, Escherichia coli O157:H7, and parasite eggs in the adults to ensure that the seed source is free from pathogen contamination. Natural vigor pre-screening is conducted through two core indicators: observing the proportion of adults actively feeding on pollen and testing the hovering time of adults in flight. Combining these two indicators, 300 individuals with strong natural vigor are selected as the initial seed source for subsequent domestication. Further, in step (2), fresh fish fermented feed is used to induce adult insects to lay eggs in a concentrated manner and to serve as the main food for 1st to 3rd instar larvae; the raw material composition of fresh fish fermented feed by weight percentage is: 60% fresh miscellaneous fish, 20% wheat bran, 10% soybean meal, 5% yeast powder, 3% compound probiotics, 1% salt, 1% trace elements, fermented until pH value ≤ 5.0. In a specific embodiment, a specially prepared fermented fresh fish feed was used for inducing adult larval oviposition and feeding larvae. The raw materials were formulated according to a set weight percentage: 60% fresh mixed fish, 20% wheat bran, 10% soybean meal, 5% yeast powder, 3% compound probiotics, 1% salt, and 1% trace elements. After all raw materials were mixed evenly, the fermentation process was initiated. Environmental conditions were monitored in real time during fermentation to ensure the stability of the fermentation system until the feed pH value dropped below 5.0 and remained stable. This fermented feed was used to induce adults to concentrate on oviposition in a dedicated oviposition area and also served as the sole staple food for 1st-3rd instar larvae, providing comprehensive and easily absorbed nutritional support for larval growth and development. Further, in step (2), the initial environmental stress is to apply the following stresses within 60 hours of the development of 1st to 3rd instar larvae: temperature stress of raising the temperature to 35±1℃ or lowering it to 20±1℃ for 2-3 hours daily during the 24th to 36th hour of the development of 3rd instar larvae; humidity stress of lowering the humidity to 40±5% or raising it to 90±5% during the 12th to 24th hour of the development of 2nd instar larvae; and ammonia concentration stress of raising the environmental ammonia concentration to 15-20ppm and continuing for 4-6 hours daily throughout the entire development of 3rd instar larvae. In a specific embodiment, the total developmental cycle of 1st-3rd instar larvae is strictly controlled within 60 hours, during which initial environmental stresses are applied in stages. For 3rd instar larvae, from the 24th to 36th hour of development, a fixed period of 2-3 hours daily is selected to raise the ambient temperature to 35°C or lower it to 20°C to implement temperature stress. For 2nd instar larvae, from the 12th to 24th hour of development, the ambient humidity is adjusted to 40% or 90% to implement humidity stress. Throughout the development of 3rd instar larvae, the ambient ammonia concentration is stabilized at 15 ppm, with a stress duration of 4-6 hours daily. After the stress period ends, individuals are selected according to set criteria, ultimately retaining those with a high-temperature mortality rate ≤15%, a low-temperature survival rate ≥80%, a dry environment survival rate ≥85%, and a high-humidity environment survival rate ≥90%, which then proceed to the next acclimatization stage. Furthermore, in steps (2) and (3), the time for releasing the animal back into the natural environment to recharge its energy is 7-10 days. In a specific embodiment, the larvae selected under initial environmental stress in step (2) are transferred to a natural transition zone to molt after reaching the end of the 3rd instar. After the adults molt, they are released into the natural environment of flower bushes to freely feed on pollen, nectar, and natural microorganisms, while absorbing sunlight and negative oxygen ions to complete energy empowerment. The release period is set at 7 days, and the egg masses laid by the adults are collected after the period expires. After the larvae selected under complex environmental stress in step (3) molt, they are released into complex natural environments such as alpine flower bushes and wetland edges, and are also given 7-10 days of natural energy empowerment time. After the adults have completed reproduction, the egg masses laid by them are collected for subsequent domestication. Further, in step (3), the combined environmental stress is to apply a combination of environmental stresses for 2-3 days during the development of 1st-3rd instar larvae. The combined environmental stresses include: a first stress combination with a temperature of 38±1℃ and a humidity of 90±5%, or a second stress combination with a temperature of 15±1℃ and a humidity of 30±5%; and an ammonia concentration stress that raises the environmental ammonia concentration to 25-30ppm and exposes the larvae to ammonia concentration for 6-8 hours per day. In a specific embodiment, step (3) involves using the egg masses recovered from the first generation as the seed source to hatch larvae, and initiating a complex environmental stress program during the 1st to 3rd instar development. The stress combinations are divided into two categories: one is a high temperature and high humidity combination of 38℃ and 90% humidity, and the other is a low temperature and low humidity combination of 15℃ and 30% humidity. Both combinations are applied continuously for 2-3 days. At the same time, the concentration of ammonia in the environment is increased to 25-30ppm, and the exposure time is guaranteed to be 6-8 hours per day. After the larvae are subjected to complex environmental stress, individuals with the required tolerance are selected and released back into the complex natural environment after molting. During the recovery stage, individuals that can recover to a normal physiological state within 2 hours after wind and rain are selected, and their egg masses are collected. Further, in step (4), the ultimate environmental stress is to apply temperature stress of 39±1℃ or 10±1℃, humidity stress of 25±5% or 98±2% and ammonia concentration stress of 35-40ppm during the development of 1st-3rd instar larvae; each stress factor is applied for 4-6 hours per day for 3 days. In a specific embodiment, step (4) uses the egg masses recovered from the second generation as the seed source to hatch 16,000 larvae. During the 1st to 3rd instar development, a multi-factor synergistic ultimate environmental stress is applied. Temperature stress is set at a high temperature group of 39℃ and a low temperature group of 10℃, and humidity stress is set at a low humidity group of 25% and a high humidity group of 98%. The ammonia concentration is kept stable at 35ppm. The three stress factors are applied simultaneously, and each stress factor is exposed for 4-6 hours per day for 3 consecutive days to test the larvae's comprehensive tolerance through extreme environment. Furthermore, in step (4), the screening criteria are: larval mortality rate ≤5%, developmental synchronicity ≥90%, and adult single-laying egg quantity ≥180 eggs. In a specific embodiment, after the ultimate environmental stress ended, the larvae were comprehensively screened according to three criteria. First, the survival rate of the larvae was statistically analyzed to ensure that the mortality rate was controlled within 5%. Second, the developmental process of the larvae was monitored by recording indicators such as molting time and body length growth to ensure that the developmental difference between batches was ≤6 hours and the developmental synchronicity reached more than 90%. Finally, after the larvae emerged as adults, their single egg-laying volume was continuously observed and recorded, and individuals with an egg-laying volume of ≥180 eggs were selected. Through layers of screening, 14,000 individuals meeting all criteria were finally selected from 16,000 larvae, among which the average actual single egg-laying volume of the adults reached 190 eggs, meeting the requirements for trait consolidation. Furthermore, in steps (2) and (3), the oviposition area for inducing adult insects to lay eggs is a sealed box containing a fish-decay substrate and a fermentation liquid containing sex pheromone analogs. In the specific embodiment, steps (2) and (3) both use a dedicated sealed box as the adult insect oviposition area. The box is pre-lined with a decaying fish substrate and a fermentation liquid containing sex pheromone analogs. Through the dual inducing effect of the substrate's odor and the sex pheromones, adults are attracted to concentrate on oviposition in the designated area. A physical wall is erected within a 1-kilometer radius of the domestication base, and biological bait containing fermented fresh fish feed is placed. This combination of physical isolation and biological trapping effectively prevents external flies from invading, ensuring the purity of the breeding stock. This design effectively increases the concentration of egg masses, reduces the difficulty of egg mass collection, and decreases the risk of egg mass contamination, ensuring the synchronicity of the hatching stage and guaranteeing the stability and consistency of the breeding stock during each generation of domestication. Furthermore, the five-grain insect population was obtained through domestication using the methods described above. In a specific embodiment, the grain insect population obtained through the complete domestication process described above met all preset standards in all core indicators. Professional testing showed that the population's SOD activity reached 850 U / mg, while the wild species' SOD activity was 650 U / mg, representing an increase of approximately 30.7%. The average prepupal weight was 0.38 g / individual, exceeding the set standard of 0.35 g / individual. Molecular identification showed a genetic similarity of 96%. Furthermore, this population demonstrated stable tolerance to environments with 39°C high temperature, 10°C low temperature, 98% high humidity, and 40 ppm ammonia, exhibiting significantly superior overall resilience and functional value compared to wild and traditionally farmed populations. It should be noted that the combination of the technical features in this case is not limited to the combination methods described in the claims of this case or the combination methods described in the specific embodiments. All technical features described in this case can be freely combined or combined in any way, unless they contradict each other. It should also be noted that the embodiments listed above are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and similar changes or modifications made thereto are those that can be directly derived or easily conceived by those skilled in the art from the content disclosed in the present invention, and should all fall within the protection scope of the present invention.

Claims

1. A method for ecocycling domestication of high quality silkworms, characterized by, The method comprises the following steps: (1) Source acquisition and identification: obtaining Chrysomya megacephala adults, performing pure strain identification and health detection, and pre-screening individuals with natural vitality as initial sources; (2) First generation basic domestication: hatching eggs obtained in step (1) and feeding 1-3 instar larvae in an artificial environment, applying initial environmental stress, and screening individuals with up-to-standard tolerance; after the individuals become adults, they are released into the natural environment for energy empowerment and reproduction, and the egg masses produced are recovered; (3) Second generation intensive domestication: hatching the egg masses recovered in step (2) and feeding 1-3 instar larvae, applying compound environmental stress, and screening individuals with up-to-standard tolerance; after the individuals become adults, they are released into a complex natural environment for energy empowerment and reproduction, and the egg masses produced are recovered; (4) Third generation stable domestication: hatching the egg masses recovered in step (3) and feeding 1-3 instar larvae, applying ultimate environmental stress, and screening according to preset mortality, development synchrony and egg production standards to obtain a domesticated population with solidified traits.

2. A method of ecocycling domesticating high quality Zophobas morio as claimed in claim 1, characterized by: In step (1): The pure strain identification comprises morphological identification based on adult morphological characteristics and molecular marker identification based on mitochondrial COI genes and microsatellite DNA markers; The health detection comprises detection of pathogenic microorganisms and parasitic worm eggs; The pre-screening of natural vitality comprises observing the pollens of adult flies and / or testing their flight endurance.

3. A method of ecocycling acclimatizing quality Zophobas morio according to claim 2, characterized by: In step (2), fresh fish fermented feed is used to induce adult flies to lay eggs and serve as the main food for 1-3 instar larvae; the raw material composition of the fresh fish fermented feed is as follows in terms of weight percentage: fresh miscellaneous fish 60%, wheat bran 20%, soybean meal 10%, yeast powder 5%, complex probiotics 3%, salt 1%, trace elements 1%, and fermentation is performed until the pH value is less than or equal to 5.

0.

4. A method of ecocycling domesticating high quality Zophobas morio as claimed in claim 3, wherein: In step (2), the initial environmental stress is to apply the following stress within 60 hours of the development of 1-3 instar larvae: temperature stress of increasing to 35±1℃ or decreasing to 20±1℃ for 2-3 hours per day at 24-36 hours of the development of 3 instar larvae; humidity stress of decreasing to 40±5% or increasing to 90±5% at 12-24 hours of the development of 2 instar larvae; and ammonia concentration stress of increasing the environmental ammonia concentration to 15-20ppm and exposing to the ammonia concentration for 4-6 hours per day.

5. A method of eco-cycling and domesticating quality Zophobas morio as claimed in claim 4, wherein: In steps (2) and (3), the time for energy empowerment in the natural environment is 7-10 days.

6. A method of ecocycling domesticating high quality Zophobas morio as claimed in claim 5, characterized by: In step (3), the compound environmental stress is to apply combined environmental stress for 2-3 days during the development of 1-3 instar larvae, the combined environmental stress comprising: a first stress combination of temperature 38±1℃ and humidity 90±5%, or a second stress combination of temperature 15±1℃ and humidity 30±5%; and ammonia concentration stress of increasing the environmental ammonia concentration to 25-30ppm and exposing to the ammonia concentration for 6-8 hours per day.

7. A method of eco-cycling and domesticating quality Zophobas morio as claimed in claim 6, wherein: In step (4), the ultimate environmental stress is temperature stress of 39±1℃ or 10±1℃, humidity stress of 25±5% or 98±2%, and ammonia concentration stress of 35-40ppm, applied during the development of 1-3 instar larvae; each stress factor is exposed for 4-6 hours per day, and lasts for 3 days.

8. A method of eco-cycling and domesticating high quality Zophobas morio as claimed in claim 7, wherein: In step (4), the screening criteria are: larval mortality ≤5%, development synchrony ≥90%, and adult single oviposition ≥180 eggs.

9. A method of eco-cycling and domesticating quality Zophobas morio as claimed in claim 8, wherein: In step (2) and step (3), the oviposition area for inducing adult oviposition is a closed box, which is provided with rotten fish substrate and fermentation liquor containing sex pheromone analogs.

10. A population of silkworm larvae obtained by domestication according to the method of any one of claims 1 to 9.

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