Theabrownin enhanced freeze-dried weight-losing granules as well as preparation method and application thereof

Abstract

The invention discloses a theabrownin enhanced freeze-dried weight-losing granule as well as a preparation method and application thereof. Comprises: theabrownin derived from a tea extract of dark tea and / or jasmine tea; an alpha-amylase inhibitor; a catechin-based substance; dietary fibers; a sweetener; wherein based on the total dry weight of the composition, the weight ratio of the theabrownin to the tea polyphenol in the tea extract is (1: 8)-(1: 20). According to the theabrownin enhanced freeze-dried weight-losing granules and the preparation method and application thereof, the freeze-dried granules have a loose porous structure and can be rapidly disintegrated and dissolved when meeting water, and the solution is uniform, stable and free of precipitation; key active ingredients (tea polyphenol, theabrownin, epigallocatechin gallate and an alpha-amylase inhibitor) are effectively protected in the production process, and the final product is high in content and clear in proportion, so that a synergistic fat reducing effect is favorably exerted; meanwhile, the product is good in stability and convenient to store and carry.

Description

Technical Field

[0001] This application relates to the technical field of food, and in particular to a theabrownin-enriched freeze-dried weight-loss granule, its preparation method, and its application. Background Technology

[0002] With the improvement of living standards and changes in dietary structure, the obese population continues to expand, leading to a growing market demand for weight-loss health products. Tea extracts, due to their natural and gentle weight-loss properties, have become a research hotspot in this field. Among them, tea polyphenols and EGCG can promote fat metabolism and inhibit fat absorption, while theaflavins can regulate adipocyte differentiation pathways and possess both antioxidant and intestinal fat adsorption capabilities. The synergistic use of these two extracts can achieve a weight-loss effect greater than the sum of its parts (1+1>2).

[0003] Currently, existing tea-based weight-loss products suffer from several technical challenges: First, theabrownins have large molecular weights and strong polarity, making them prone to aggregation in aqueous systems, resulting in poor product solubility and precipitation after brewing, which affects the consumption experience and the absorption efficiency of active ingredients; Second, active ingredients such as tea polyphenols and theabrownins are sensitive to temperature and oxygen, and traditional production processes (such as high-temperature extraction and atmospheric pressure mixing) easily cause oxidation and loss of these components, significantly reducing the product's weight-loss efficacy; Some products add large amounts of sucrose and flavorings to improve taste, which contradicts the need for weight loss and fails to address the slight bitterness inherent in theabrownins.

[0004] Furthermore, existing freeze-dried tea granule preparation processes have significant shortcomings: uneven mixing of materials before freeze-drying easily leads to localized aggregation of theaflavins; excessively high pre-freezing temperatures and insufficient vacuum result in coarse ice crystal structures that disrupt the loose, porous morphology of the granules, affecting their instant solubility; and the lack of effective oxygen-freezing measures throughout the production process allows active ingredients to continue oxidizing during crushing and packaging, further reducing product quality. Summary of the Invention

[0005] This application aims to at least partially address one of the technical problems in the related art.

[0006] Therefore, one objective of this application is to provide a theabrownin-enhanced freeze-dried weight-loss granule, its preparation method, and its application. The freeze-dried granules have a loose and porous structure, which can quickly disintegrate and dissolve in water, resulting in a uniform, stable solution without precipitation. The key active ingredients (tea polyphenols, theabrownins, epigallocatechin gallate, and α-amylase inhibitors) are effectively protected during the production process, resulting in high content and a clear ratio in the final product, which is conducive to exerting a synergistic fat-reducing effect. At the same time, the product has good stability and is easy to store and carry.

[0007] To achieve the above objectives, the first aspect of this application provides a theabrownin-enhanced freeze-dried weight-loss granule, comprising: Theabrownin; Tea extract, derived from dark tea and / or jasmine tea, wherein the tea extract contains tea polyphenols; α-Amylase inhibitor; Catechins; Dietary fiber; Sweeteners; Wherein, based on the total dry weight of the composition, the weight ratio of the theabrownin to the tea polyphenols in the tea extract is 1:8 to 1:20.

[0008] In addition, the theabrownin-enhanced freeze-dried weight-loss granules, their preparation method, and their application as described above in this application may also have the following additional technical features: In one embodiment of this application, the ingredients, by weight, include the following: 10.5 parts of black tea-jasmine tea concentrate, 0.8 parts of pure theabrownin, 2.5 parts of white kidney bean extract, 0.5 parts of green tea extract, 2.8 parts of resistant dextrin, 1.7 parts of erythritol-stevioside compound, and 1.2 parts of fructooligosaccharide-maltodextrin compound. The black tea-jasmine tea concentrate is obtained by mixing black tea and jasmine tea at a weight ratio of 8:2 and extracting the concentrate. The black tea-jasmine tea concentrate has a solid content of 48% and a tea polyphenol content of ≥85mg / g. The purity of the theabrownin is ≥92%, and the molecular weight is ≥5000 Da; The white kidney bean extract has an α-amylase inhibitor activity ≥4200 U / g and a protein content ≥80%. The purity of epigallocatechin gallate ester in the green tea extract is ≥65%, and the total catechin content is ≥80%. The weight ratio of erythritol to steviol glycosides in the erythritol-stevioside compound is 9:1; The weight ratio of fructooligosaccharides to maltodextrin in the fructooligosaccharide-maltodextrin compound is 2:1.

[0009] In one embodiment of this application, the particles are freeze-dried under vacuum to form a loose porous solid, and the inner walls of the pores and the framework of the particles contain theabrownins, tea polyphenols and epigallocatechin gallate.

[0010] In one embodiment of this application, the theabrownin exists in an amorphous form, and the primary particle size of the theabrownin is ≤500nm; The particles have a moisture content of ≤1.2% and a bulk density of 0.25-0.45 g / cm³.

[0011] In one embodiment of this application, based on a specification of 20g / piece, the total content of the four components—tea polyphenols, α-amylase inhibitors, epigallocatechin gallate, and theabrownins—in each piece is ≥5.8g.

[0012] The second aspect of this application discloses a method for preparing theabrownin-enhanced freeze-dried weight-loss granules, the method comprising the following steps: S1 raw material pretreatment: The pure theabrownin was pulverized at -5℃ and passed through a 150-mesh sieve and a 200-mesh sieve in sequence to control the particle size to ≤75μm; The white kidney bean extract and green tea extract were passed through a 120-mesh sieve respectively. Grind the raw materials of black tea and jasmine tea into 30-40 mesh respectively; All raw materials were weighed under a temperature of 18-20℃, relative humidity of ≤30%, and nitrogen micro-positive pressure environment. Pure theabrownins were weighed under nitrogen protection. Preparation of S2 tea concentrate: The black tea and jasmine tea raw materials processed in step S1 were added to pure water at a material-to-liquid ratio of 1:35, and extracted at low temperature and 250W power for 60 minutes using ultrasonic extraction. After filtration, filtrate A and filter residue were obtained. Add the filter residue to pure water at a material-to-liquid ratio of 1:30, and extract again for 50 minutes at 35℃ and 250W power. Filter to obtain filtrate B. Combine filtrate A and filtrate B, and concentrate them under vacuum at 40℃ and -0.098MPa until the solid content is 48%. Cool to 10℃ to obtain black tea-jasmine tea concentrate. S3 Mixing and Blending: The black tea-jasmine tea concentrate obtained in step S2 was transferred into a nitrogen-protected low-temperature stirring tank. The theabrownins obtained in step S1 were added, and the mixture was first sheared and dispersed at a high speed of 1500 r / min for 20 min, and then stirred at a low speed of 40 r / min for 10 min. Add the white kidney bean extract and green tea extract processed in step S1, stir at 50 r / min for 40 min, and test the uniformity of theabrownin dispersion RSD ≤ 2%; Add resistant dextrin, erythritol-stevioside complex, and fructooligosaccharide-maltodextrin complex, and stir at 35 r / min for 30 min, controlling the content deviation of all active ingredients within ±1.5%; S4 freeze-drying molding: The mixture obtained in step S3 is loaded into a material tray, and the thickness of the tray is controlled to be 10-12 mm. Place the tray in a -50℃ environment for quick freezing for 5 hours; The quick-frozen material was transferred to a freeze-drying chamber with a vacuum degree ≤5Pa and a temperature of -10℃ for sublimation drying for 16 hours. The sublimated and dried material was further desorbed and dried at 30℃ for 8 hours, with the final moisture content controlled to be ≤1.2%. S5 Grinding, Sieving, and Packaging: The freeze-dried material obtained in step S4 is crushed under nitrogen protection and passed through a 120-mesh sieve. The product is packaged in 20g units, using vacuum aluminum foil packaging material. Nitrogen gas is introduced before sealing to control the residual oxygen content inside the packaging to be ≤1%. Heat sealing is performed at 120℃, and the sealing strength of the seal is controlled to be ≥35N / 15mm. In step S1, an electronic scale with an accuracy of ≥0.001g is used to weigh the raw materials, and the weighed raw materials are immediately transferred to a sealed container for storage.

[0013] In addition, the theabrownin-enhanced freeze-dried weight-loss granules, their preparation method, and their application as described above in this application may also have the following additional technical features: In one embodiment of this application, the sublimation drying and desorption drying in step S4 are performed using a high-precision vacuum freeze dryer with multi-point temperature monitoring function; In step S5, the crushing and sieving are carried out using a sealed drying hopper equipped with a nitrogen circulation system.

[0014] In one embodiment of this application, the low-temperature ultrasonic extraction in step S2 is performed under constant temperature of 35°C and power of 250W. The vacuum concentration was carried out under constant temperature of 40℃ and vacuum degree of -0.098MPa.

[0015] In one embodiment of this application, the high-speed shear dispersion in step S3 is carried out at a speed of 1500 r / min for 20 min; the low-speed stirring is carried out at a speed of 40 r / min for 10 min.

[0016] The third aspect of this application proposes the application of a theabrownin-enhanced freeze-dried slimming granule as a weight-loss health product or food.

[0017] Beneficial effects: The theabrownin-enhanced freeze-dried slimming granules, their preparation method, and their application in this application are as follows: The freeze-dried granules have a loose and porous structure, which can quickly disintegrate and dissolve upon contact with water, resulting in a uniform, stable solution without precipitation; the key active ingredients (tea polyphenols, theabrownins, epigallocatechin gallate, and α-amylase inhibitors) are effectively protected during the production process, resulting in high content and a clear ratio in the final product, which is conducive to exerting a synergistic fat-reducing effect; at the same time, the product has good stability and is easy to store and carry.

[0018] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0019] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the following description of the embodiments taken in conjunction with the accompanying drawings, wherein: Figure 1 This is a flowchart illustrating the preparation method of the theabrownin-enhanced freeze-dried weight-loss granules, their preparation method, and their application, according to one embodiment of this application. Detailed Implementation

[0020] Embodiments of this application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this application, and should not be construed as limiting this application. Rather, embodiments of this application include all variations, modifications, and equivalents falling within the spirit and scope of the appended claims.

[0021] The following is in conjunction with the appendix Figure 1 This application describes the theabrownin-enhanced freeze-dried weight-loss granules, their preparation method, and their application.

[0022] Example 1: The theabrownin-enhanced freeze-dried weight-loss granules provided in this application include: Theabrownin; Tea extract, derived from dark tea and / or jasmine tea, wherein the tea extract contains tea polyphenols; α-Amylase inhibitor; Catechins; Dietary fiber; Sweeteners; The weight ratio of theaflavins to tea polyphenols in the tea extract is 1:8 to 1:20, based on the total dry weight of the composition.

[0023] Further, by weight, it contains the following ingredients: 10.5 parts of black tea-jasmine tea concentrate, 0.8 parts of pure theabrownin, 2.5 parts of white kidney bean extract, 0.5 parts of green tea extract, 2.8 parts of resistant dextrin, 1.7 parts of erythritol-stevioside compound, and 1.2 parts of fructooligosaccharide-maltodextrin compound; Among them, the black tea-jasmine tea concentrate is obtained by mixing black tea and jasmine tea at a weight ratio of 8:2 and extracting the concentrate. The solid content of the black tea-jasmine tea concentrate is 48%, and the tea polyphenol content is ≥85mg / g. The purity of theabrownin is ≥92%, and the molecular weight is ≥5000 Da; The α-amylase inhibitor activity of white kidney bean extract is ≥4200 U / g, and the protein content is ≥80%. The purity of epigallocatechin gallate ester in green tea extract is ≥65%, and the total catechin content is ≥80%. The weight ratio of erythritol to steviol glycosides in the erythritol-stevioside compound is 9:1. The weight ratio of fructooligosaccharides to maltodextrin in the fructooligosaccharide-maltodextrin compound is 2:1.

[0024] Furthermore, based on a specification of 20g / strip, the total content of the four components—tea polyphenols, α-amylase inhibitors, epigallocatechin gallate, and theabrownins—in each granule is ≥5.8g.

[0025] Formula: Weigh the following ingredients: 10.5 kg of concentrated black tea-jasmine tea extract, 0.8 kg of pure theabrownin, 2.5 kg of white kidney bean extract, 0.5 kg of green tea extract, 2.8 kg of resistant dextrin, 1.7 kg of a sweetener composed of erythritol and steviol glycosides in a 9:1 ratio, and 1.2 kg of a mixture of fructooligosaccharides and maltodextrin in a 2:1 ratio. Based on the total dry weight of the above ingredients, the weight ratio of theabrownin to tea polyphenols is 1:10, falling within the range of 1:8 to 1:20.

[0026] Raw material standards: The black tea-jasmine tea concentrate used was prepared by mixing black tea and jasmine tea in a weight ratio of 8:2, followed by extraction and concentration. The solid content was found to be 48%, and the tea polyphenol content was 90mg / g.

[0027] The purity of theabrownin was 93%, and the weight-average molecular weight was 5500 Da (determined by GPC method).

[0028] The α-amylase inhibitor activity of white kidney bean extract was 4300 U / g, and the protein content was 82%.

[0029] The green tea extract contains 66% epigallocatechin gallate and 82% total catechins.

[0030] Preparation process: Pretreatment: Pure theabrownin was pulverized at -5℃ and passed through 150-mesh and 200-mesh sieves, with a particle size D90 of 70μm. White kidney bean extract and green tea extract were passed through a 120-mesh sieve. Black tea and jasmine tea were pulverized to 35 mesh. All operations were carried out under a nitrogen-filled slightly positive pressure environment at 20℃ and humidity <30%.

[0031] Concentrate: Black tea and jasmine tea raw materials were mixed with water at a ratio of 1:35 and ultrasonically extracted at 35℃ and 250W for 60 minutes. The residue was then re-extracted with water at a ratio of 1:30 for 50 minutes. The filtrates were combined and concentrated under vacuum at 40℃ and -0.098MPa to a solid content of 48%, and then cooled to 10℃.

[0032] Mixing: The concentrate was transferred to a nitrogen-protected stirred tank. Theabrownins were added, and the mixture was sheared at 1500 rpm for 20 minutes, followed by stirring at 40 rpm for 10 minutes. White kidney bean and green tea extracts were added, and the mixture was stirred at 50 rpm for 40 minutes. The RSD of theabrownin dispersion was measured to be 1.8%. The remaining excipients were added, and the mixture was stirred at 35 rpm for 30 minutes. The deviation of the active ingredient content was ≤ ±1.3%.

[0033] Freeze-drying: The material was packed into trays (11 mm thick) and quick-frozen at -50°C for 5 hours. It was then transferred to a refrigerated dryer (vacuum degree 4 Pa) and sublimated at -10°C for 16 hours, followed by desorption drying at 30°C for 8 hours. The moisture content was measured to be 1.0%.

[0034] Packaging: Crushed through a 120-mesh sieve under nitrogen protection, packaged in 20g / strip nitrogen-filled (0.8% residual oxygen) aluminum foil packaging, heat-sealed at 120℃, with a sealing strength of 38N / 15mm.

[0035] Product specifications: According to testing, the total content of the four core components—tea polyphenols, α-amylase inhibitors, epigallocatechin gallate, and theabrownins—is 5.9g per 20g of finished product granules, which is ≥5.8g.

[0036] The ingredient list is compiled based on the production of 1000 strips, each weighing 20g, with a total feed of 20kg. Example 2, in one embodiment of this application, the particles are freeze-dried under vacuum to form a loose porous solid, and the inner walls of the pores and the skeleton of the particles contain theabrownins, tea polyphenols and epigallocatechin gallate.

[0037] The particle samples obtained in Example 1 were observed using a scanning electron microscope (SEM). The results showed that the particles exhibited a typical loose and porous solid morphology with a well-developed pore structure. Energy dispersive spectroscopy (EDS) surface scanning revealed that the characteristic elemental signals of theaflavins, tea polyphenols, and epigallocatechin gallate were uniformly distributed in the inner walls of the pores and the framework of the particles, indicating that these active ingredients were fully dispersed in the porous network structure.

[0038] Example 3, in one embodiment of this application, theabrownin exists in an amorphous form, and the primary particle size of theabrownin is ≤500nm; The granules have a moisture content of ≤1.2% and a bulk density of 0.25-0.45 g / cm³. They can completely disintegrate and dissolve in 300 ml of water at 25℃ within 30 seconds.

[0039] Phase and morphology: The particles from Example 1 were subjected to X-ray diffraction (XRD) analysis. The spectrum showed broadened "bun-shaped" peaks in the range of 5°-40° (2θ), with no sharp crystalline diffraction peaks, indicating that the theaflavins existed in an amorphous form. Wet particle size analysis using a laser particle size analyzer showed that the primary particle size D90 after dispersion in water was 450 nm, ≤500 nm.

[0040] Physical properties: The measured moisture content of the particles was 1.0%, ≤1.2%; the bulk density was 0.32 g / cm³, between 0.25-0.45 g / cm³.

[0041] Solubility: Take one strip (20g) of this product and place it in a beaker containing 300ml of purified water at 25℃. Let it stand and observe. The granules rapidly disintegrate and completely dissolve within 28 seconds, resulting in a homogeneous and clear solution with no visible precipitate.

[0042] This application discloses a method for preparing the above-mentioned theabrownin-enhanced freeze-dried weight-loss granules, the method comprising the following steps: S1 raw material pretreatment: The pure theabrownin was pulverized at -5℃ and passed through a 150-mesh sieve and a 200-mesh sieve in sequence to control the particle size to ≤75μm; The white kidney bean extract and green tea extract were passed through a 120-mesh sieve respectively. Grind the raw materials of black tea and jasmine tea into 30-40 mesh respectively; All raw materials were weighed under a temperature of 18-20℃, relative humidity of ≤30%, and nitrogen micro-positive pressure environment. Pure theabrownins were weighed under nitrogen protection. Preparation of S2 tea concentrate: The black tea and jasmine tea raw materials processed in step S1 were added to pure water at a material-to-liquid ratio of 1:35, and extracted at low temperature and 250W power for 60 minutes using ultrasonic extraction. After filtration, filtrate A and filter residue were obtained. Add the filter residue to pure water at a material-to-liquid ratio of 1:30, and extract again for 50 minutes at 35℃ and 250W power. Filter to obtain filtrate B. Combine filtrate A and filtrate B, and concentrate them under vacuum at 40℃ and -0.098MPa until the solid content is 48%. Cool to 10℃ to obtain black tea-jasmine tea concentrate. S3 Mixing and Blending: The black tea-jasmine tea concentrate obtained in step S2 was transferred into a nitrogen-protected low-temperature stirring tank. The theabrownins obtained in step S1 were added, and the mixture was first sheared and dispersed at a high speed of 1500 r / min for 20 min, and then stirred at a low speed of 40 r / min for 10 min. Add the white kidney bean extract and green tea extract processed in step S1, stir at 50 r / min for 40 min, and test the uniformity of theabrownin dispersion RSD ≤ 2%; Add resistant dextrin, erythritol-stevioside complex, and fructooligosaccharide-maltodextrin complex, and stir at 35 r / min for 30 min, controlling the content deviation of all active ingredients within ±1.5%; S4 freeze-drying molding: The mixture obtained in step S3 is loaded into a material tray, and the thickness of the tray is controlled to be 10-12 mm. Place the tray in a -50℃ environment for quick freezing for 5 hours; The quick-frozen material was transferred to a freeze-drying chamber with a vacuum degree ≤5Pa and a temperature of -10℃ for sublimation drying for 16 hours. The sublimated and dried material was further desorbed and dried at 30℃ for 8 hours, with the final moisture content controlled to be ≤1.2%. S5 Grinding, Sieving, and Packaging: The freeze-dried material obtained in step S4 is crushed under nitrogen protection and passed through a 120-mesh sieve. The product is packaged in 20g units, using vacuum aluminum foil packaging material. Nitrogen gas is introduced before sealing to control the residual oxygen content inside the packaging to be ≤1%. Heat sealing is performed at 120℃, and the sealing strength of the seal is controlled to be ≥35N / 15mm. In step S1, an electronic scale with an accuracy of ≥0.001g is used to weigh the raw materials, and the weighed raw materials are immediately transferred to a sealed container for storage.

[0043] This process aims to improve upon existing processing techniques and address the problems encountered in current manufacturing processes.

[0044] Systematic control of oxygen-containing environments.

[0045] Throughout key processes such as raw material weighing, mixing and dispersion, and pulverization and packaging, a slightly positive pressure environment under nitrogen protection is maintained. This measure aims to continuously reduce the risk of oxidation reactions of components such as tea polyphenols, epigallocatechin gallate, and theabrownins during production, providing a fundamental condition for maintaining the content of active ingredients in the final product.

[0046] Targeted improvement of the dispersion state of theaflavins.

[0047] The method first involves pulverizing and sieving theabrownin at a low temperature (-5℃) to control its initial physical particle size (≤75μm). Subsequently, in the mixing stage, a high-shear device (1500 r / min) is used to process the mixture of theabrownin and tea concentrate. This step aims to utilize mechanical shear force to promote the deagglomeration and dispersion of theabrownin particles in the liquid phase, creating conditions for its uniform distribution in the final product.

[0048] The product structure and performance can be adjusted by controlling the process parameters of freeze-drying.

[0049] The method involves rapid freezing at -50℃ to form fine ice crystals, followed by sublimation drying under high vacuum (≤5Pa) and low temperature (-10℃). These process conditions effectively remove moisture while maintaining the mixed and dispersed state of the material in the liquid state to a solid state, forming particles with a porous structure. This porous structure, along with the uniform distribution of the active ingredients, contributes to the product's low bulk density and rapid disintegration characteristics.

[0050] Preferably, in one embodiment of this application, the sublimation drying and desorption drying in step S4 are carried out using a high-precision vacuum freeze dryer with multi-point temperature monitoring function; In step S5, the crushing and sieving are carried out using a sealed drying hopper equipped with a nitrogen circulation system.

[0051] In the preparation process of Example 1: the sublimation and desorption drying in step S4 were carried out using a high-precision vacuum freeze dryer (model: LYO-series) with multi-point temperature monitoring function to monitor the temperature of different points of the material in real time and ensure uniform drying.

[0052] The crushing and sieving in step S5 is carried out in a sealed drying hopper (model: FZ-series) equipped with a nitrogen circulation system, maintaining a low-oxygen environment throughout the process to prevent the material from absorbing moisture and oxidizing.

[0053] Preferably, in one embodiment of this application, the low-temperature ultrasonic extraction in step S2 is performed under constant temperature of 35°C and power of 250W. Vacuum concentration was carried out under constant temperature of 40℃ and vacuum degree of -0.098MPa.

[0054] The extraction and concentration parameters in step S2 are designed to ensure high yield and high stability of the active ingredients.

[0055] Low-temperature ultrasonic extraction (35℃, 250W): This parameter combination is a mild and efficient extraction solution for heat-sensitive and oxygen-sensitive active ingredients such as tea polyphenols and theabrownins.

[0056] 35℃ constant temperature: Compared with the traditional hot water extraction at 60-80℃, this temperature can maximally inhibit the oxidation and isomerization of polyphenols and the thermal denaturation of theaflavins, thus protecting their activity from the source.

[0057] 250W ultrasonic power: Ultrasonic cavitation effect can efficiently break down plant cell walls, significantly improving extraction efficiency and shortening extraction time. This application found through experiments that 250W is the power that can generate sufficient cavitation intensity at a low temperature of 35℃ without causing localized overheating and disrupting the equilibrium of components due to excessive power. Too low a power results in insufficient extraction, while too high a power may lead to degradation of active ingredients.

[0058] Low-temperature vacuum concentration (40℃, -0.098MPa): This parameter is a measure to protect the active ingredients during the concentration stage.

[0059] The synergy of 40℃ and -0.098MPa high vacuum: Under this vacuum level, the boiling point of water can be lowered to below approximately 30℃. Controlling the temperature at 40℃ ensures that the concentration process proceeds rapidly in a low-temperature environment far below the atmospheric pressure boiling point (100℃), effectively avoiding the oxidation and polymerization of tea polyphenols, the epitaxial transformation of EGCG, and the destruction of the molecular structure of theaflavins caused by prolonged high-temperature heating.

[0060] Preferably, in one embodiment of this application, in step S3, the high-speed shear dispersion is carried out at a speed of 1500 r / min for 20 min; and the low-speed stirring is carried out at a speed of 40 r / min for 10 min.

[0061] The high-speed shear dispersion stage (1500 r / min, 20 min) aims to overcome the strong aggregation tendency of theaflavins due to their large molecular weight and high surface polarity by using high-intensity mechanical shear force. The rotation speed of 1500 r / min provides sufficient shear energy to effectively break down the theaflavin aggregates and gradually disperse them into the tea concentrate, forming a preliminary homogeneous suspension. The 20-min duration ensures that the shear energy is fully applied to the entire material system, allowing the dispersion process to reach a stable state and laying the foundation for subsequent mixing.

[0062] The low-speed stirring stage (40 r / min, 10 min) is initiated immediately after high-speed shearing and then switched to low-speed stirring. The main purposes are threefold: Eliminating eddies and bubbles: High-speed shearing can easily introduce air and generate violent eddies. Low-speed stirring helps to stabilize the material flow field, remove entrained gas, and prevent bubbles from affecting the pore structure and product appearance in the subsequent freeze-drying process.

[0063] Promotes system homogeneity and stability: At lower shear rates, materials can achieve further micro-homogeneity through gentle movement, while avoiding localized temperature rises or potential mechanical damage to dispersed active ingredients caused by continuous high shear.

[0064] To create suitable conditions for the next step of feeding: the material system under low-speed stirring is in a stable and uniform state, which facilitates the subsequent addition and mixing of ingredients such as white kidney bean extract and green tea extract, and ensures that each component is evenly distributed.

[0065] This application discloses the application of the above-mentioned theabrownin-enhanced freeze-dried slimming granules as a weight-loss health product or food.

[0066] The theaflavin-enhanced freeze-dried weight-loss granules prepared in Example 1 were combined with appropriate nutrients (such as vitamins and minerals) according to the recommended dosage of 1-2 times daily, and then filled into capsules or directly packaged into small sachets to prepare a weight-loss health product for assisting weight management. This product is convenient to carry, quick-dissolving, and easily absorbed.

[0067] In summary, the theabrownin-enhanced freeze-dried weight-loss granules, their preparation method, and their application in this application demonstrate that the freeze-dried granules have a loose, porous structure, can rapidly disintegrate and dissolve upon contact with water, and produce a uniform, stable solution without precipitation. The key active ingredients (tea polyphenols, theabrownins, epigallocatechin gallate, and α-amylase inhibitors) are effectively protected during the production process, resulting in high content and a clear ratio in the final product, which is beneficial for synergistic weight-loss effects. Simultaneously, the product exhibits good stability and is easy to store and carry.

[0068] In the description of this specification, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0069] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0070] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A theabrownin-enriched freeze-dried weight-loss granule, characterized in that, include: Theabrownin; Tea extract, derived from dark tea and / or jasmine tea, wherein the tea extract contains tea polyphenols; α-Amylase inhibitor; Catechins; Dietary fiber; Sweeteners; Wherein, based on the total dry weight of the composition, the weight ratio of the theabrownin to the tea polyphenols in the tea extract is 1:8 to 1:

20.

2. The theabrownin-enhanced freeze-dried weight-loss granules according to claim 1, characterized in that, By weight, it contains the following ingredients: 10.5 parts black tea-jasmine tea concentrate, 0.8 parts pure theabrownin, 2.5 parts white kidney bean extract, 0.5 parts green tea extract, 2.8 parts resistant dextrin, 1.7 parts erythritol-stevioside compound, and 1.2 parts fructooligosaccharide-maltodextrin compound; The black tea-jasmine tea concentrate is obtained by mixing black tea and jasmine tea at a weight ratio of 8:2 and extracting the concentrate. The black tea-jasmine tea concentrate has a solid content of 48% and a tea polyphenol content of ≥85mg / g. The purity of the theabrownin is ≥92%, and the molecular weight is ≥5000 Da; The white kidney bean extract has an α-amylase inhibitor activity ≥4200 U / g and a protein content ≥80%. The purity of epigallocatechin gallate ester in the green tea extract is ≥65%, and the total catechin content is ≥80%. The weight ratio of erythritol to steviol glycosides in the erythritol-stevioside compound is 9:1; The weight ratio of fructooligosaccharides to maltodextrin in the fructooligosaccharide-maltodextrin compound is 2:

1.

3. The theabrownin-enhanced freeze-dried weight-loss granules according to claim 1, characterized in that, The particles are freeze-dried under vacuum to form a loose, porous solid. The inner walls of the pores and the framework of the particles contain theabrownins, tea polyphenols, and epigallocatechin gallate.

4. The theabrownin-enhanced freeze-dried slimming granules according to claim 1, characterized in that, The theabrownin exists in an amorphous form, and the primary particle size of the theabrownin is ≤500nm. The particles have a moisture content of ≤1.2% and a bulk density of 0.25-0.45 g / cm³.

5. The theabrownin-enhanced freeze-dried weight-loss granules according to claim 1, characterized in that, Based on a specification of 20g / strip, each granule contains a total content of ≥5.8g of four components: tea polyphenols, α-amylase inhibitors, epigallocatechin gallate, and theabrownins.

6. A method for preparing the theabrownin-enhanced freeze-dried weight-loss granules according to any one of claims 1-5, characterized in that, The method includes the following steps: S1 raw material pretreatment: The pure theabrownin was pulverized at -5℃ and passed through a 150-mesh sieve and a 200-mesh sieve in sequence to control the particle size to ≤75μm; The white kidney bean extract and green tea extract were passed through a 120-mesh sieve respectively. Grind the raw materials of black tea and jasmine tea into 30-40 mesh respectively; All raw materials were weighed under a temperature of 18-20℃, relative humidity of ≤30%, and nitrogen micro-positive pressure environment. Pure theabrownins were weighed under nitrogen protection. Preparation of S2 tea concentrate: The black tea and jasmine tea raw materials processed in step S1 were added to pure water at a material-to-liquid ratio of 1:35, and extracted at low temperature with ultrasonication for 60 minutes at 35℃ and 250W power. After filtration, filtrate A and filter residue were obtained. Add the filter residue to pure water at a material-to-liquid ratio of 1:30, and extract again for 50 minutes at 35℃ and 250W power. Filter to obtain filtrate B. Combine filtrate A and filtrate B, and concentrate them under vacuum at 40℃ and -0.098MPa until the solid content is 48%. Cool to 10℃ to obtain black tea-jasmine tea concentrate. S3 Mixing and Blending: The black tea-jasmine tea concentrate obtained in step S2 was transferred into a nitrogen-protected low-temperature stirring tank. The theabrownins obtained in step S1 were added, and the mixture was first sheared and dispersed at a high speed of 1500 r / min for 20 min, and then stirred at a low speed of 40 r / min for 10 min. Add the white kidney bean extract and green tea extract processed in step S1, stir at 50 r / min for 40 min, and test the uniformity of theabrownin dispersion RSD ≤ 2%; Add resistant dextrin, erythritol-stevioside complex, and fructooligosaccharide-maltodextrin complex, and stir at 35 r / min for 30 min, controlling the content deviation of all active ingredients within ±1.5%; S4 freeze-drying molding: The mixture obtained in step S3 is loaded into a material tray, and the thickness of the tray is controlled to be 10-12 mm. Place the tray in a -50℃ environment for quick freezing for 5 hours; The quick-frozen material was transferred to a freeze-drying chamber with a vacuum degree ≤5Pa and a temperature of -10℃ for sublimation drying for 16 hours. The sublimated and dried material was further desorbed and dried at 30℃ for 8 hours, with the final moisture content controlled to be ≤1.2%. S5 Grinding, Sieving, and Packaging: The freeze-dried material obtained in step S4 is crushed under nitrogen protection and passed through a 120-mesh sieve. The product is packaged in 20g units, using vacuum aluminum foil packaging material. Nitrogen gas is introduced before sealing to control the residual oxygen content inside the packaging to be ≤1%. Heat sealing is performed at 120℃, and the sealing strength of the seal is controlled to be ≥35N / 15mm. In step S1, an electronic scale with an accuracy of ≥0.001g is used to weigh the raw materials, and the weighed raw materials are immediately transferred to a sealed container for storage.

7. The method according to claim 6, characterized in that, In step S4, sublimation drying and desorption drying are carried out using a high-precision vacuum freeze dryer with multi-point temperature monitoring function; In step S5, the crushing and sieving are carried out using a sealed drying hopper equipped with a nitrogen circulation system.

8. The method according to claim 6, characterized in that, The low-temperature ultrasonic extraction described in step S2 is performed under constant temperature of 35℃ and power of 250W. The vacuum concentration was carried out under constant temperature of 40℃ and vacuum degree of -0.098MPa.

9. The method according to claim 6, characterized in that, In step S3, the high-speed shear dispersion is carried out at a speed of 1500 r / min for 20 min; the low-speed stirring is carried out at a speed of 40 r / min for 10 min.

10. The application of the theabrownin-enhanced freeze-dried slimming granules according to any one of claims 1-5 as a weight-loss health product or food.

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