Application of beer vinasse hydrolysate in mask base solution
The preparation of beer lees hydrolysate by hydrolyzing beer lees with acidic protease solves the problems of waste of beer lees resources and environmental pollution, provides a new raw material for face masks, and improves the antioxidant effect and sustainability of face masks.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-05
- Publication Date
- 2026-03-31
AI Technical Summary
Inadequate treatment and utilization of brewer's grains leads to resource waste and environmental pollution, while also hindering the sustainability and cost-effectiveness of mask raw materials.
A beer tank hydrolysate with antioxidant activity was prepared by hydrolyzing beer tanks with acidic protease. This hydrolysate was then used in a facial mask base solution. The hydrolysis temperature, time, and enzyme dosage were adjusted to improve efficiency and purity.
This technology enables the high-value application of brewer's grains, provides a new source of raw materials for the face mask industry, improves the antioxidant effect of face masks, reduces environmental impact, and meets the high standards required for face mask raw materials.
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Figure CN121754466A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of facial mask technology, and more particularly to the application of a beer lees hydrolysate in facial mask base liquid. Background Technology
[0002] With the booming development of the beer industry, brewer's grains, as a major byproduct of beer brewing, are produced in considerable quantities. Currently, there are many shortcomings in the treatment and utilization of brewer's grains. Typically, brewer's grains are used directly as animal feed. However, the protein in brewer's grains exists in large molecular form, making it difficult for animal digestive systems to fully digest and absorb it. Studies have shown that animals typically only utilize 30-40% of the protein in brewer's grains, resulting in a significant waste of nutrients. Furthermore, the nutritional composition of brewer's grains fluctuates seasonally with changes in brewing raw materials and processes, directly leading to unstable growth performance in animals fed brewer's grains. For example, the weight gain rate of livestock and poultry fed brewer's grains can vary by 10-20% in different seasons, severely impacting farming efficiency.
[0003] Some brewer's grains are directly discarded or landfilled as waste, a practice that poses a significant threat to the environment. Brewer's grains are rich in organic matter, and their decomposition in the natural environment produces large amounts of greenhouse gases such as methane. It is estimated that landfilling one ton of brewer's grains can produce approximately 10-15 cubic meters of methane annually. Methane's greenhouse effect is about 25 times that of carbon dioxide, significantly exacerbating global warming. Furthermore, some harmful substances in brewer's grains, such as heavy metal residues and microbial metabolic toxins, may be washed into the soil and groundwater by rainwater, leading to soil compaction, decreased fertility, pollution of surrounding water bodies, and disruption of the ecological balance.
[0004] Attempts have been made to extract energy from the combustion of brewer's grains, but due to their relatively low calorific value, the combustion efficiency is low. Furthermore, the combustion process releases harmful gases such as sulfur dioxide and nitrogen oxides, which are significant contributors to acid rain and smog. According to relevant monitoring data, burning one ton of brewer's grains produces approximately 1-2 kg of sulfur dioxide and 0.5-1 kg of nitrogen oxides, causing severe air pollution.
[0005] In the cosmetics industry, the demand for face masks continues to grow. Currently, the main active ingredients in face mask bases are mostly chemically synthesized substances, plant extracts, or animal extracts. While chemically synthesized substances are highly effective, they pose certain risks of irritation and safety. Plant extracts often require large amounts of plant resources, putting pressure on the ecological environment; for example, some rare plants are endangered due to over-harvesting. Animal extracts may trigger allergic reactions, and their acquisition process may involve animal ethics issues.
[0006] Therefore, if beer lees can be converted into cosmetic raw materials, especially for the production of face masks, it can not only solve the problem of waste disposal in the beer industry and realize the recycling of resources, but also open up new raw material sources for face mask products and enhance the market competitiveness of the products. Summary of the Invention
[0007] The purpose of this invention is to provide an application of beer tank hydrolysate in facial mask base liquid, addressing issues such as low utilization efficiency of beer tanks, insufficient sustainability of facial mask raw materials, and cost-effectiveness.
[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides an application of beer tank hydrolysate in facial mask base liquid, wherein the volume ratio of beer tank hydrolysate to facial mask base liquid is 5~15:100.
[0009] Preferably, the method for preparing the beer tank hydrolysate includes the following steps: 1) Add water to the beer tank and homogenize it to obtain a beer tank homogenate; 2) Add acidic protease to the beer tank homogenate and hydrolyze it to obtain beer tank hydrolysate.
[0010] Preferably, the mass ratio of beer tank to water in step 1) is 1:1.5~2.5.
[0011] Preferably, the mass-to-volume ratio of the acidic protease and the beer vat homogenate in step 2) is 0.1~0.3g:100mL.
[0012] Preferably, the hydrolysis temperature in step 2) is 40~50℃ and the hydrolysis time is 5~7h.
[0013] Preferably, after the hydrolysis in step 2), the process further includes sequential high-temperature inactivation and centrifugation to collect the supernatant, thereby obtaining the beer tank hydrolysate.
[0014] Preferably, the high-temperature inactivation temperature is 60~80℃, and the high-temperature inactivation time is 20~40min.
[0015] Preferably, the centrifugal force is 10000~12000g and the centrifugation time is 5~15min.
[0016] Preferably, the hydrolysis in step 2) is carried out by stirring at a speed of 150~250 r / min.
[0017] The beneficial effects of this invention are: 1) This invention utilizes acidic protease to hydrolyze beer tanks into small-molecule peptides and amino acids with antioxidant activity, solving the problem of beer tank resource waste, realizing the high-value application of beer tanks, reducing the environmental pressure caused by improper disposal of beer tanks, and providing a new and sustainable source of raw materials for the facial mask industry. By precisely controlling the hydrolysis temperature, time, and amount of acidic protease, the acidic protease can exert its maximum catalytic activity under the most suitable environment, improving the hydrolysis efficiency of beer tanks, ensuring the stability and antioxidant activity of the beer tank hydrolysate, effectively removing free radicals in the skin, delaying skin aging, and improving skin radiance and elasticity. Centrifugation effectively removes impurities, improving the purity and quality of the beer tank hydrolysate, meeting the high standards required for facial mask raw materials.
[0018] 2) The method of the present invention does not require high-end equipment, the conditions are mild, easy to control, and the cost is low, which is conducive to industrial promotion and application. Attached Figure Description
[0019] Figure 1 The comparison of the DPPH removal rate of the brewer's tank hydrolysate prepared in Example 1 and Comparative Examples 1-3; Figure 2 The comparison of the scavenging rates of hydroxyl radicals by the brewer's tank hydrolysate prepared in Example 1 and Comparative Examples 1-3; Figure 3 The comparison of the ABTS removal rate of the beer tank hydrolysate prepared in Example 1 and Comparative Examples 1-3 is shown. Detailed Implementation
[0020] This invention provides an application of beer tank hydrolysate in facial mask base liquid, wherein the volume ratio of beer tank hydrolysate to facial mask base liquid is 5~15:100.
[0021] In this invention, the volume ratio of the beer tank hydrolysate to the facial mask base is preferably 7~13:100, more preferably 9~11:100, and even more preferably 10:100.
[0022] In this invention, the method for preparing the beer tank hydrolysate preferably includes the following steps: 1) Add water to the beer tank and homogenize it to obtain a beer tank homogenate; 2) Add acidic protease to the beer tank homogenate and hydrolyze it to obtain beer tank hydrolysate.
[0023] In this invention, the mass ratio of beer tank to water in step 1) is preferably 1:1.5~2.5, more preferably 1:1.8~2.2, and even more preferably 1:2.
[0024] In this invention, the preferred mass-to-volume ratio of the acidic protease and the beer tank homogenate in step 2) is 0.1-0.3 g: 100 mL, more preferably 0.15-0.25 g: 100 mL, and even more preferably 0.2 g: 100 mL. The acidic protease hydrolyzes the beer tank, degrading large molecules such as proteins into bioactive small peptides and amino acids, thus giving the beer tank hydrolysate antioxidant activity.
[0025] In this invention, the hydrolysis temperature in step 2) is preferably 40~50℃, more preferably 42~48℃, and even more preferably 45℃; the hydrolysis time is preferably 5~7h, more preferably 5.5~6.5h, and even more preferably 6h.
[0026] In this invention, after the hydrolysis in step 2), it is preferable to further include sequentially performing high-temperature inactivation and centrifuging to collect the supernatant to obtain beer tank hydrolysate.
[0027] In this invention, the temperature for high-temperature inactivation is preferably 60-80°C, more preferably 65-75°C, and even more preferably 70°C; the time for high-temperature inactivation is preferably 20-40 min, more preferably 25-35 min, and even more preferably 30 min.
[0028] In this invention, the centrifugal force is preferably 10000~12000g, more preferably 10500~11500g, and even more preferably 11000g; the centrifugation time is preferably 5~15min, more preferably 8~12min, and even more preferably 10min.
[0029] In this invention, after centrifugation, the solution is preferably filtered through a microporous membrane with a pore size of 0.22 μm to obtain beer tank hydrolysate.
[0030] In this invention, the hydrolysis in step 2) is preferably carried out by stirring, and the stirring speed is preferably 150~250 r / min, more preferably 180~220 r / min, and even more preferably 200 r / min.
[0031] In this invention, the mask base preferably further comprises water, a humectant, a thickener, and an additive.
[0032] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0033] The acidic protease (50000 U / g) used in the embodiments and comparative examples of this invention was purchased from Sichuan Lvcheng Biotechnology Co., Ltd., and the beer tanks were from Tsingtao Brewery (Taiyuan) Co., Ltd.
[0034] Example 1
[0035] Add 200 mL of purified water to 100 g of beer tank and homogenize using a homogenizer to obtain a beer tank homogenate. Take 100 mL of the beer tank homogenate and add 0.2 g of acidic protease. Hydrolyze at 45 °C and 180 r / min for 6 h. Then raise the temperature to 70 °C and inactivate at 70 °C for 30 min. Subsequently, centrifuge at 12000 g for 10 min. Filter the supernatant through a 0.22 μm microporous membrane to obtain the beer tank hydrolysate.
[0036] The mask base liquid is composed of the following ingredients by volume fraction: 10% brewer's lees hydrolysate, 2% glycerin, 5% butylene glycol, 0.2% hydroxyethyl cellulose, 0.15% sodium hyaluronate, 0.1% allantoin, 4% niacinamide, and the balance is purified water.
[0037] Example 2
[0038] Add 150 mL of purified water to 100 g of beer tank and homogenize using a homogenizer to obtain a beer tank homogenate. Take 100 mL of the beer tank homogenate and add 0.3 g of acidic protease. Hydrolyze at 50 °C and 200 r / min for 5 h. Then raise the temperature to 80 °C and inactivate at 80 °C for 20 min. Subsequently, centrifuge at 11000 g for 15 min. Filter the supernatant through a 0.22 μm microporous membrane to obtain the beer tank hydrolysate.
[0039] The mask base liquid is composed of the following ingredients by volume fraction: 10% brewer's lees hydrolysate, 2% glycerin, 5% butylene glycol, 0.2% hydroxyethyl cellulose, 0.15% sodium hyaluronate, 0.1% allantoin, 4% niacinamide, and the balance is purified water.
[0040] Example 3
[0041] Add 250 mL of purified water to 100 g of beer tank and homogenize using a homogenizer to obtain a beer tank homogenate. Take 100 mL of the beer tank homogenate and add 0.1 g of acidic protease. Hydrolyze at 40 °C and 250 r / min for 7 h. Then raise the temperature to 60 °C and inactivate at 60 °C for 40 min. Centrifuge at 10000 g for 5 min. Filter the supernatant through a 0.22 μm microporous membrane to obtain the beer tank hydrolysate.
[0042] The mask base liquid is composed of the following ingredients by volume fraction: 10% brewer's lees hydrolysate, 2% glycerin, 5% butylene glycol, 0.2% hydroxyethyl cellulose, 0.15% sodium hyaluronate, 0.1% allantoin, 4% niacinamide, and the balance is purified water.
[0043] Comparative Example 1
[0044] The difference from Example 1 is that 100 mL of beer tank homogenate was taken and 0.5 g of acidic protease was added.
[0045] Comparative Example 2
[0046] The difference from Example 1 is that hydrolysis was carried out at 55°C and a rotation speed of 180 r / min for 5 h.
[0047] Comparative Example 3
[0048] The difference from Example 1 is that hydrolysis was carried out at 30°C and a rotation speed of 180 r / min for 7 h.
[0049] The in vitro antioxidant activity of the brewery hydrolysates from Example 1 and Comparative Examples 1-3 was tested. The brewery hydrolysates were prepared into samples with concentrations of 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, and 5 mg / mL using anhydrous ethanol.
[0050] The method for detecting the scavenging ability of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radicals was as follows: 1 mL of beer tank hydrolysate sample of different concentrations was added to 1 mL of DPPH, mixed thoroughly, and allowed to stand at room temperature in the dark for 30 min. Anhydrous ethanol was used as a blank control, and vitamin C (VC) was used as a positive control. The absorbance value at 517 nm was measured using a visible light spectrophotometer. The DPPH scavenging rate was calculated according to Formula I: DPPH scavenging rate = [1-(A2-A1) / A0]×100%, where A0 is the absorbance value of the anhydrous ethanol + DPPH solution, A1 is the absorbance value of beer tank hydrolysate sample of different concentrations, and A2 is the absorbance value of DPPH + beer tank hydrolysate sample of different concentrations. Figure 1 The comparison of the DPPH removal rate of the beer tank hydrolysate prepared in Example 1 and Comparative Examples 1-3 is shown.
[0051] The method for detecting hydroxyl radical scavenging ability was as follows: 0.5 mL of 9 mmol / L FeSO4 and 0.5 mL of 8.8 mmol / L hydrogen peroxide were added to a 5 mL centrifuge tube and gently shaken to mix. Then, 0.5 mL of beer lees hydrolysate samples of different concentrations were added, and finally, 0.5 mL of 9 mmol / L salicylic acid-ethanol solution was added. After mixing thoroughly, the mixture was incubated at room temperature for 30 min. VC was used as a positive control, and the absorbance at 510 nm was measured using a visible light spectrophotometer. The hydroxyl radical scavenging rate is calculated according to Formula II: hydroxyl radical scavenging rate = [1-(A2-A1) / A0]×100%, where A0 is the absorbance value of the FeSO4 + salicylic acid-ethanol solution + hydrogen peroxide solution, A1 is the absorbance value of different concentrations of beer tank hydrolysate sample + FeSO4 + salicylic acid-ethanol solution, and A2 is the absorbance value of hydrogen peroxide + FeSO4 + salicylic acid-ethanol solution + different concentrations of beer tank hydrolysate sample. Figure 2 The comparison of the scavenging rate of hydroxyl radicals by the brewer's tank hydrolysate prepared in Example 1 and Comparative Examples 1-3 is shown.
[0052] The method for detecting the scavenging ability of 2,2-adiazon-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) was as follows: 1.9 mL of ABTS working solution was added to 0.1 mL of brewery hydrolysate samples of different concentrations, mixed well, and allowed to stand at room temperature in the dark for 6 min. VC was used as a positive control, and the absorbance value at 734 nm was measured using a visible light spectrophotometer. The ABTS scavenging rate was calculated according to Formula III: ABTS scavenging rate = [1-(A2-A1) / A0]×100%, where A0 is the absorbance value of anhydrous ethanol + ABTS working solution, A1 is the absorbance value of brewery hydrolysate samples of different concentrations + anhydrous ethanol, and A2 is the absorbance value of ABTS working solution + brewery hydrolysate samples of different concentrations. Figure 3 The comparison of the ABTS removal rate of the beer tank hydrolysate prepared in Example 1 and Comparative Examples 1-3 is shown.
[0053] Depend on Figures 1-3 It can be seen that the in vitro antioxidant activity of the beer tank hydrolysate of Example 1 is significantly higher than that of Comparative Examples 1-3.
[0054] Sensory evaluation, physicochemical property testing, microbial testing, and heavy metal testing were performed on the mask base liquids of Example 1 and Comparative Examples 1-3, respectively.
[0055] Sensory evaluation includes moisturizing (whether it provides a moisturizing feeling), greasiness (whether it is too greasy), spreadability (how easy it is to move on the skin surface after application), absorbability (from application to complete absorption), and stickiness (the stickiness of the skin after complete absorption). The rating levels of sensory evaluation are shown in Table 1, and the sensory evaluation results are shown in Table 2.
[0056] The physicochemical properties were tested, including pH value, heat resistance, and cold resistance. pH value was tested and evaluated according to the "Cosmetic Safety Technical Specifications." The heat resistance test conditions were: maintaining the temperature at 40℃ for 24 hours, and then observing whether stratification occurred after returning to room temperature. The cold resistance test conditions were: maintaining the temperature at -5℃ for 24 hours, and then observing whether stratification occurred after returning to room temperature. The results of the physicochemical properties tests are shown in Table 3.
[0057] Microbiological and heavy metal testing was conducted in accordance with the "Cosmetic Safety Technical Specifications". Microbiological testing results are shown in Table 4, and heavy metal testing results are shown in Table 5.
[0058] Table 1. Sensory evaluation rating levels
[0059] Table 2 Results of sensory evaluation
[0060] Table 3. Results of Physicochemical Properties Tests
[0061] Table 4 Microbial test results
[0062] Table 5 Heavy metal detection results
[0063] As shown in Tables 2-5, Example 1 strictly followed the optimal process parameters (enzyme dosage 0.2g / 100mL, hydrolysis temperature 45℃, time 6h, etc.) to prepare the beer tank hydrolysate. Therefore, the mask base liquid of Example 1 had the best sensory evaluation (all 5 points), stable physicochemical properties, and fully met the standards for microorganisms and heavy metals. Comparative Example 1 had an excessive enzyme dosage (0.5g / 100mL), resulting in an excess of some components, slight stratification, and a small number of colonies. Comparative Example 2 (temperature 55℃) and Comparative Example 3 (temperature 30℃) deviated from the optimal hydrolysis temperature, resulting in insufficient enzyme activity, lower sensory scores, and slight stratification in cold / heat resistance. The heavy metal detection results showed that Example 1 and Comparative Examples 1-3 were all far below the limit standard, which complies with the "Cosmetic Safety Technical Specifications". In the microbial detection, only Comparative Examples 1 and 3 showed a small number of colony totals (not exceeding the standard), which may be related to slight fluctuations in system stability caused by enzyme dosage or temperature control. The pH values were all within the skin's suitable range (5.5-6.5), ensuring that the product is mild and non-irritating.
[0064] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. Use of a beer tank hydrolysate in a mask base fluid, characterized in that, The volume ratio of the beer tank hydrolysate and the mask base fluid is 5-15:
100.
2. Use of the beer lauter's wort hydrolysate according to claim 1 in a mask base fluid, characterized in that, The preparation method of the beer tank hydrolysate comprises the following steps: 1) homogenizing the beer tank with water to obtain beer tank homogenate; 2) adding acid protease to the beer tank homogenate to hydrolyze, thereby obtaining the beer tank hydrolysate.
3. Use of the beer lauter's wort hydrolysate according to claim 2 in a mask base fluid, characterized in that, In step 1), the mass ratio of the beer tank and water is 1:1.5-2.
5.
4. Use of a beer lauter's wort hydrolysate in a facial mask base fluid according to claim 2 or 3, characterized in that, In step 2), the mass-volume ratio of the acid protease and the beer tank homogenate is 0.1-0.3 g:100 mL.
5. Use of the beer lauter's wort hydrolysate according to claim 4 in a mask base fluid, characterized in that, In step 2), the temperature of the hydrolysis is 40-50 ℃, and the hydrolysis time is 5-7 h.
6. Use of the beer lauter's wort hydrolysate according to claim 5 in a mask base fluid, characterized in that, In step 2), after the hydrolysis, high-temperature inactivation is sequentially performed, and the supernatant is collected by centrifugation to obtain the beer tank hydrolysate.
7. Use of the beer lauter's wort hydrolysate according to claim 6 in a mask base fluid, characterized in that, The temperature of the high-temperature inactivation is 60-80 ℃, and the high-temperature inactivation time is 20-40 min.
8. Use of the beer lauter's wort hydrolysate according to claim 6 or 7 in a mask base fluid, characterized in that, The centrifugal force of the centrifugation is 10,000-12,000 g, and the centrifugation time is 5-15 min.
9. Use of the beer lauter's wort hydrolysate according to claim 8 in a mask base fluid, characterized in that, In step 2), the hydrolysis is performed under stirring, and the stirring speed is 150-250 r / min.