Multi-enzyme cleaning agent and application thereof

Abstract

The invention relates to a multienzyme cleaning agent and application thereof, discusses the compounding type and concentration ratio of a chelating agent and a surfactant, selects EPO as the surfactant and the chelating agent MGDA, under the compounding condition, the EPO and the chelating agent MGDA can play a synergistic role in a mass ratio of (25-30): (3-7), and the multienzyme cleaning agent shows an excellent cleaning effect in removal of artificially simulated pollutants, bacteria and ATP (adenosine triphosphate).

Description

Technical Field

[0001] This invention relates to the field of medical device cleaning agents, specifically to multi-enzyme cleaning agents and their applications. Background Technology

[0002] Cleaning of medical devices is a crucial step in hospital infection control, directly impacting patient safety and the quality of medical care. Multi-enzyme cleaning solutions have become the mainstream choice for medical device cleaning due to their highly efficient ability to decompose organic contaminants. Surfactants are one of the core components of multi-enzyme cleaning solutions. Their main function is to significantly improve cleaning effectiveness by reducing the surface tension of the liquid, enhancing the wettability, penetration, and emulsification ability of the cleaning solution. Especially when dealing with complex biological contaminants, such as proteins, lipids, starches, and bacterial biofilms, surfactants can effectively disrupt the structure of these substances, thereby enhancing the catalytic activity of enzymes. However, commercially available cleaning agents may not be effective due to formulation issues that prevent the surfactants from fully exerting their effects, or the surfactants, enzymes, and chelating agents failing to achieve optimal cleaning results. Summary of the Invention

[0003] To address the technical deficiencies of existing technologies, this invention provides a multi-enzyme cleaning agent and its applications.

[0004] The technical solution adopted in this invention is a multi-enzyme cleaning agent, which contains biological enzymes, surfactants, chelating agents, auxiliaries and solvents. The surfactant is EPO and the chelating agent is MGDA. The mass ratio of surfactant EPO to chelating agent MGDA in the medical multi-enzyme cleaning agent is 25-30:3-7.

[0005] The bioenzyme mentioned is one or more of cellulase, amylase, lipase, and protease.

[0006] The additives include propylene glycol, sodium benzoate, sodium formate, and benzotriazole.

[0007] The mass ratio of cellulase, amylase, lipase, and protease is 1:2:3:6.

[0008] Multi-enzyme cleaning agent, by weight percentage, comprises the following components: 3-7% MGDA, 25-35% EPO, 10% propylene glycol, 0.5% sodium benzoate, 0.5% sodium formate, 0.5% benzotriazole, 0.5% cellulase, 1% amylase, 1.5% lipase, 3% protease, balance water.

[0009] Application of a multi-enzyme cleaning agent in the preparation of cleaning reagents for medical devices.

[0010] The beneficial effects of this invention are as follows: This invention provides a multi-enzyme cleaning agent and its application, explores the types and concentration ratios of chelating agents and surfactants, selects EPO as a surfactant and MGDA as a chelating agent, and under the condition of compounding, the two can play a synergistic role at a mass ratio of 25-30:3-7, showing excellent cleaning effect in the removal of artificially simulated pollutants, bacteria and ATP. Attached Figure Description

[0011] Figure 1 The cleaning effect of different chelating agents on artificially simulated pollutants in Example 1 is shown.

[0012] Figure 2 The effect of different chelating agents on bacterial removal in Example 1 is shown.

[0013] Figure 3 The ATP decrease rate after washing with different chelating agents in Example 1 is shown.

[0014] Figure 4 The cleaning effect of different surfactants on artificially simulated pollutants in Example 2.

[0015] Figure 5 The effect of different surfactants on bacterial removal in Example 2 is shown.

[0016] Figure 6 The ATP reduction rate after washing with different surfactants in Example 2 is shown.

[0017] Figure 7 The image shows the ATP residues remaining after different surfactants were used to clean the simulated equipment in Example 3. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1: Preparation of multi-enzyme cleaning agents with different types and concentrations of chelating agents

[0020] Example 2: Preparation of multi-enzyme cleaning agents with different types and concentrations of surfactants

[0021] Example 3: Verification of Cleaning Effect 1) Simulate the effect of pollutant cleaning Purchase commercially available simulated contaminants that conform to ISO 15883 standards, dilute the cleaning solution at a ratio of 1:400, take 300ml and put it into a glass jar, and keep it at a constant temperature of 40°C in a water bath.

[0022] Three simulated contaminants were placed into three glass jars containing cleaning solution and covered. The jars were rotated and washed at a speed of 45 to 60 rpm for 10 minutes. The jars were then removed, the simulated contaminants were taken out, and the jars were hung on a drying rack and stored at 30°C for 2 hours. The weight was recorded as m1, and the cleaning effect was visually assessed.

[0023] After weighing the contaminated tablets, clean them using the following steps: a) Soak in water containing detergent for 30 minutes; b) Clean in ultrasonic water for 30 minutes; c) Rinse with purified water; d) Boil in water containing detergent for 30 minutes; e) Rinse with tap water; f) Boil in distilled water for 10 minutes; g) Rinse with distilled water until the pH is neutral; h) After hanging on the drying rack for 30 minutes and then in the desiccator overnight, weigh each group of samples, which is m2.

[0024] The artificially simulated pollutant removal rate is calculated using equation (D.1): ...............(D.1) In the formula: R — Simulated pollutant removal rate, % m0 — Simulated mass of contaminants before cleaning, in milligrams (mg); m1 — The simulated mass of contaminants after cleaning with medical cleaning agent, expressed in milligrams (mg). m2 — The simulated mass of contaminants after washing, expressed in milligrams (mg). 2) Removal effect on mixtures of blood and bacteria The cleaning effect of the cleaning solution was tested according to the method in Appendix C of T / WSJD002-2019, and the bacterial removal rate and ATP reduction rate were calculated. The dilution ratio of the cleaning solution was 1:400.

[0025] 3) Simulates the cleaning effect of surgical instruments Artificial contaminants were prepared by mixing enterococci, pig blood, lard, BSA, and starch. Surgical scissors were immersed in the contaminants for 4 hours, then removed and air-dried overnight.

[0026] The cleaning solution was diluted at a ratio of 1:400. The contaminated surgical scissors were then immersed in the cleaning solution and cleaned for 30 minutes using a machine-washing spray method. The cleaning effect of the surgical scissors was then observed with the naked eye.

[0027] After repeatedly wiping the instrument surface with a sterilized cotton swab, cut off the swab tip and immerse it in 10 ml of PBS solution, then shake thoroughly. Detect the ATP content. The ATP test method is the same as in 4).

[0028] Cleaning results: (1) Using EPO as a surfactant, the cleaning effects of different chelating agents at various concentration gradients were compared. For example, from... Figures 1-3 As shown, the cleaning effect increases with increasing chelating agent concentration. However, different types of chelating agents have a significant impact on the cleaning effect. Among them, MGDA showed superior cleaning performance compared to the other three chelating agents in the removal of artificially simulated pollutants, bacteria, and ATP.

[0029] (2) The above experimental results show that MGDA has a good cleaning effect. Therefore, MGDA was used as a chelating agent for formula fixation to compare the cleaning effects of different surfactants at various concentration gradients. Figures 4-7 As shown, various types of surfactants exhibited good cleaning effects in removing artificially simulated contaminants. Compared with commercially available products, the 25% and 35% concentrations of EPO and the 35% concentration of APG all exceeded those of commercially available products. Regarding bacterial removal rates, increasing the surfactant concentration significantly increased the bacterial removal rate; above 25% concentration, most surfactant groups showed better cleaning effects than commercially available groups. However, in terms of ATP reduction rate, the EPO group was significantly better than other groups. The EPO group also demonstrated excellent cleaning effects on simulated instruments. Therefore, in summary, the combination of 25-35% EPO concentration and MGDA chelating agent can significantly enhance the cleaning effect.

[0030] Please note to all technical personnel: Although the present invention has been described according to the specific embodiments above, the inventive concept of the present invention is not limited to this invention. Any modifications that utilize the inventive concept will be included within the scope of protection of this patent.

[0031] The above description is merely a preferred embodiment of the present invention, and the scope of protection of the present invention is not limited to the above embodiments. All technical solutions falling within the scope of the present invention's concept are within the scope of protection of the present invention. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of the present invention should also be considered within the scope of protection of the present invention.

Claims

1. A multi-enzyme cleaning agent, characterized in that, The multi-enzyme cleaning agent contains biological enzymes, surfactants, chelating agents, auxiliaries, and solvents. The surfactant is EPO, and the chelating agent is MGDA. The mass ratio of surfactant EPO to chelating agent MGDA in the medical multi-enzyme cleaning agent is 25-30:3-7.

2. The multi-enzyme cleaning agent according to claim 1, characterized in that, The bioenzyme mentioned is one or more of cellulase, amylase, lipase, and protease.

3. The multi-enzyme cleaning agent according to claim 1, characterized in that, The additives include propylene glycol, sodium benzoate, sodium formate, and benzotriazole.

4. The multi-enzyme cleaning agent according to claim 2, characterized in that, The mass ratio of cellulase, amylase, lipase, and protease is 1:2:3:

6.

5. The multi-enzyme cleaning agent according to claim 2, characterized in that, By mass percentage, it includes the following components: 3-7% MGDA, 25-35% EPO, 10% propylene glycol, 0.5% sodium benzoate, 0.5% sodium formate, 0.5% benzotriazole, 0.5% cellulase, 1% amylase, 1.5% lipase, 3% protease, balance water.

6. The use of the multi-enzyme cleaning agent according to claim 1 in the preparation of cleaning reagents for medical devices.

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