Microimaging system and stimulated radiation loss microscope

By designing a ring-shaped partitioned phase modulation element and a bandpass thin film, the problems of coaxial stability and optical energy loss in traditional stimulated emission loss microscopes were solved, achieving stable spot overlap and system miniaturization, and improving image quality and signal-to-noise ratio.

CN121763550APending Publication Date: 2026-03-31INST OF CHEM CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202610043054.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-14
Publication Date
2026-03-31

AI Technical Summary

Technical Problem

Traditional stimulated emission depletion microscopes suffer from problems such as poor coaxial stability, wavelength sensitivity, high light energy loss, and large system size, which affect the image signal-to-noise ratio and system miniaturization.

Method used

A ring-shaped partitioned phase modulation element is used to modulate and converge the phase of the first and second beams incident coaxially, forming a one-to-one corresponding light spot with the center overlapping. The high numerical aperture illumination objective is eliminated, and a low numerical aperture collection objective is used, combined with a bandpass thin film to selectively transmit a specific wavelength beam.

Benefits of technology

It achieves long-term stable overlap of light spots, reduces optical energy loss, miniaturizes the system, improves image quality and signal-to-noise ratio, and is adaptable to commercial lasers with large bandwidth and wavelength drift.

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Abstract

The invention belongs to the technical field of optical microscopic imaging, and discloses a microscopic imaging system and a stimulated radiation loss microscope. The microscopic imaging system comprises an illumination mechanism used for providing a first light beam and a second light beam which are coaxially incident; the annular partition phase modulation element comprises a first phase modulation area and a second phase modulation area connected to the peripheral side of the first phase modulation area, the first phase modulation area is circular, and the second phase modulation area is annular; the first phase modulation area and the second phase modulation area are configured to perform phase modulation and convergence on a first light beam and a second light beam which are vertically incident respectively, so that a first light spot and a second light spot which are in one-to-one correspondence and coincide in center are formed on the focal plane of the stimulated radiation loss microscope respectively. According to the technical scheme, light energy loss caused when the microscope adopts a high-numerical-aperture illumination objective lens is improved, miniaturization of the system is facilitated, and the imaging quality is improved.
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