Poultry immunological enhancement composition based on vitamin mannan oligosaccharide and bioactive peptide and application of poultry immunological enhancement composition

Abstract

The invention discloses a poultry immunological enhancement composition based on vitamin mannan oligosaccharide and bioactive peptide and application of the poultry immunological enhancement composition, and belongs to the technical field of livestock and poultry breeding. Through creative experiments, the optimal mass ratio of the vitamin mannan oligosaccharide to the bioactive peptide is determined. In the early growth stage of poultry, the two components are combined according to a specific ratio for use, and a synergistic interaction effect is generated in the aspect of improving the ND antibody level of the poultry. And compared with the use of a single component, the feed additive can significantly improve the growth of poultry and improve the immune organ index. According to the invention, a solution with more economic benefits is provided for realizing antibiotic-free breeding, and the requirements of green, healthy and sustainable development of modern animal husbandry are met.

Description

Technical Field

[0001] This invention relates to the field of livestock and poultry breeding technology, specifically to an immune-enhancing composition for poultry based on vitanol oligosaccharides and bioactive peptides, and its application. Background Technology

[0002] Poultry farming provides humans with an important source of animal protein, such as meat and eggs. However, with the continuous development of large-scale farming models, various stress factors in the farming environment (such as high density, flock transfer, vaccination, and temperature changes) exacerbate immunosuppression in poultry, leading to declining chick quality and increased susceptibility to pathogens. Traditional solutions mainly rely on adding antibiotics to feed to prevent disease and promote growth. However, the long-term overuse and residue problems of antibiotics are becoming increasingly serious, not only leading to the emergence of drug-resistant strains but also severely affecting the quality and safety of livestock and poultry products. Therefore, developing safe, efficient, and residue-free green immune enhancers has become a research hotspot and urgent need in the livestock industry.

[0003] Currently, research and development efforts seeking antibiotic alternatives mainly focus on areas such as probiotics, enzyme preparations, plant extracts, and functional oligosaccharides. In recent years, researchers have begun exploring the combined use of different types of immune enhancers to achieve synergistic effects. However, different active ingredients may exhibit physical or chemical incompatibility, or interfere with each other's pathways of action in vivo. Currently, no research has revealed whether there is a synergistic effect between vitanol oligosaccharides and specific bioactive peptides, and there is a lack of scientifically validated optimized formulations and application schemes that can clearly guide production. This makes it difficult to promote their application in industrial production. Therefore, there is an urgent need in this field to develop a novel and effective antibiotic alternative that is clearly defined in its composition, scientifically formulated, precisely dosed, and has stable effects, suitable for poultry, and capable of rapidly promoting the development of their immune system in the early stages of poultry growth, thus providing healthy poultry farming. Summary of the Invention

[0004] In view of the above-mentioned prior art, the purpose of this invention is to provide an immune-enhancing composition for poultry based on vitanol oligosaccharides and bioactive peptides and its application, thereby improving poultry immunity, promoting the development of immune organs and improving growth performance.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a composition for improving the immunity of poultry, wherein the mass ratio of vitanol oligosaccharide and bioactive peptide in the composition is 1:(1-2).

[0006] Preferably, the mass ratio of vitanol oligosaccharide to bioactive peptide in the composition is 1:1.

[0007] The preparation method of the bioactive peptide includes the following steps: (1) Weigh out Astragalus membranaceus, Polygonatum sibiricum and glycosides, mix them evenly, add distilled water and extract in a water bath at 90-95℃, and collect the filtrate; (2) Add liquid excipients to the filtrate, adjust the pH to 5.5-6.5, autoclave, and obtain liquid culture medium; (3) Inoculate the Bacillus subtilis culture solution into the liquid culture medium at a volume ratio of 5%-10% and ferment to obtain bioactive peptides.

[0008] In a second aspect, the present invention provides the use of the above composition in any one of the following (1)-(4): (1) Preparation of feed additives for improving ND antibody levels in poultry; (2) Prepare feed additives for promoting the development of poultry immune organs; (3) Prepare feed additives to improve the survival rate of poultry; (4) Prepare feed additives for improving the feed conversion ratio and increasing the slaughter weight of poultry.

[0009] Preferably, the poultry is broiler chicken.

[0010] Preferably, the feed additive may also contain feed-grade acceptable carriers or excipients.

[0011] Preferably, the feed-grade acceptable carrier is selected from one or more of wheat bran, rice husk powder, zeolite powder, silica, and calcium carbonate.

[0012] Preferably, the feed additive is in the form of powder, granules, or liquid.

[0013] In a third aspect, the present invention provides a method for improving the immunity and growth performance of chicks, comprising the following steps: Weigh 15-20g of the composition and dissolve it in 250mL of water to obtain a composition solution. Feed the chicks the composition solution by drinking water for 10-14 days during the growth stage from hatching to 3 weeks of age.

[0014] Preferably, the composition is fed to chicks during the period from 0 to 14 days of age, and the daily feeding dose of the composition is (0.01-0.015 mL) / chick.

[0015] The beneficial effects of this invention are: This invention, through inventive experiments, determined the optimal mass ratio between vitanol oligosaccharides and bioactive peptides. Using these two components in a specific ratio during the early stages of poultry growth produced a synergistic effect in increasing ND antibody levels in poultry. Furthermore, compared to using either component alone, it significantly improved poultry growth and immune organ indices. This invention provides a more economical solution for antibiotic-free farming, meeting the requirements of green, healthy, and sustainable development in modern animal husbandry. Attached Figure Description

[0016] Figure 1 For dead-out rate detection.

[0017] Figure 2 For detecting trends in weight change.

[0018] Figure 3 This represents the trend of changes in the feed conversion ratio.

[0019] Figure 4 To detect the development of the bursa of Fabricius.

[0020] Figure 5 To assess spleen development.

[0021] Figure 6 For the detection of intestinal development. Detailed Implementation

[0022] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0023] In recent years, the quality of chicks has shown a significant downward trend, with immunosuppressive diseases being particularly prominent. These diseases have seriously affected the health of the flock and the final slaughter indicators, causing significant losses to poultry production.

[0024] Based on this, the present invention develops an immune-enhancing composition for poultry based on vitanyl oligosaccharides and bioactive peptides, and its application. Research has confirmed that the rational use of a composition containing vitanyl oligosaccharides and bioactive peptides in the early stages of chick rearing can effectively promote the development of chick immune organs, improve broiler growth performance, and enhance broiler resistance to Newcastle disease virus.

[0025] The specific embodiments of the present invention will be described in further detail below with reference to examples. The following detailed descriptions are illustrative and intended to provide further explanation of this application, rather than limiting the scope of the invention.

[0026] The vegan oligosaccharides used in the examples were purchased from Xi'an Tailuxing Biotechnology Co., Ltd. The Bacillus subtilis used were purchased from Beijing Kezhan Biotechnology Co., Ltd., product number BNCC109047.

[0027] The liquid excipients used in the examples consist of: glucose: 2.0% (w / v), peptone: 1.0% (w / v), KH2PO4: 0.1% (w / v), MgSO4·7H2O: 0.05% (w / v), and NaCl: 0.5% (w / v).

[0028] The preparation method of the bioactive peptides used in the examples includes the following steps: Example 1: Preparation of a composition for enhancing poultry immunity 1. Preparation methods of bioactive peptides (1) Accurately weigh 20g of Astragalus membranaceus, 10g of Polygonatum sibiricum, and 20g of glycosides and mix them evenly. Add 500mL of distilled water to the mixed herbs. Place in a 90℃ water bath and heat for 1.5 hours, stirring once every 20 minutes during the process.

[0029] (2) Collect the filtrate and filter it using a Buchner funnel and filter paper to obtain a clear basic extract of the medicinal materials.

[0030] (3) Take 300 mL of the basic extract of the medicinal material, add 300 mL of liquid excipients, stir evenly, adjust pH=6, autoclave at 121℃ for 20 minutes, cool to obtain liquid culture medium; inoculate the Bacillus subtilis inoculum at a volume ratio of 5% (v / v) into the liquid culture medium, and culture at 37℃ and 180 rpm for 72 hours to obtain bioactive peptides.

[0031] 2. Preparation of the composition The vitanol oligosaccharide and bioactive peptide were prepared into a composition at a mass ratio of 1:1.

[0032] Example 2: Preparation of a composition for enhancing poultry immunity 1. Preparation methods of bioactive peptides Same as Example 1.

[0033] 2. Preparation of the composition The oligosaccharides of vitanol and bioactive peptides were prepared into a composition at a mass ratio of 1:2.

[0034] Example 3: Experimental Design This experiment was conducted at Xinhe Fourth Farm. To ensure the scientific rigor and accuracy of the experiment, a controlled variable method was used to set up experimental and control groups. Four chicken houses with the same chick source and similar ages of arrival were selected for both groups. Houses 1 and 5 were designated as the experimental group, and houses 4 and 6 as the control group, with two houses in each group. Specific groupings and related information are shown in Table 1.

[0035] Table 1: Experimental Grouping Experimental treatment: Weigh 15g of the composition prepared in Example 1 and dissolve it in 250mL of water to obtain a composition solution. The experimental group was fed with 20,000 chickens / 250mL of water for 14 consecutive days starting from 1 day old; the control group was fed the same amount of water during the same period, and all other management measures were exactly the same as those of the experimental group.

[0036] Example 4: On-site growth index assessment in broiler farms Based on the experiment in Example 3, the mortality rate, body weight, and feed conversion ratio of the two groups of chickens were statistically analyzed at 7, 14, 21, and 28 days of age, respectively. The data are shown in Table 2.

[0037] Table 2: Statistics on mortality rate, body weight, and feed conversion ratio of chicken flocks (1) Trend of death rate: From Table 1 and Figure 1 It is evident that the overall mortality rate in the experimental group was lower than that in the control group, and the difference became more pronounced with increasing age. At 28 days of age, the mortality rate in Experimental Building 1 was 2.23%, and in Experimental Building 5 it was 2.91%, while in Control Buildings 4 and 6 it reached 3.31% and 4.37%, respectively. The average mortality rate of the control group was about 1.2 percentage points higher than that of the experimental group, indicating that the combined use of vitanol oligosaccharides and bioactive peptides can effectively reduce the risk of early mortality in broilers.

[0038] (2) Weight change trend: The weight of the two groups of broilers showed a steady increase overall, with little difference in weight at each age. At 7 days old, the weight of the experimental group was slightly higher than that of the control group. From 14 to 28 days old, the weight of each group fluctuated alternately without obvious regularity, indicating that the combined use of vitanol oligosaccharide and bioactive peptides had no significant direct effect on the early weight gain of broilers.

[0039] (3) Trend of feed conversion ratio: The feed conversion ratio reflects the feed conversion efficiency of broilers. The lower the value, the higher the efficiency. At 7 days old, the feed conversion ratio of the experimental group (0.82, 0.84) was lower than that of the control group (0.88, 0.87). From 14 days old to 21 days old, the feed conversion ratio of each group changed alternately. At 28 days old, the feed conversion ratio of the four chicken houses was basically the same. Overall, the feed conversion efficiency of the experimental group was slightly better than that of the control group in the early stage, and the difference narrowed in the middle and late stages.

[0040] Example 5: Detection of Immune Organs The development of immune organs (bursa of Fabricius and spleen) directly determines the strength of broiler immunity. Meanwhile, intestinal development is closely related to nutrient absorption and immune function. Based on the experiment in Example 3, this study measured the bursa of Fabricius weight and bursa-to-body ratio, spleen weight and spleen index, and intestinal length of chickens at 14 days, 21 days, and 28 days of age. The specific data are as follows.

[0041] (1) Development of the bursa of Fabricius The bursa of Fabricius is a central immune organ unique to poultry, and its weight and bursa-to-body ratio (bursa of Fabricius weight to body weight) reflect the development and maturation of B lymphocytes. Data showed that at 14 days of age, the bursa of Fabricius weight (0.93g) and bursa-to-body ratio (0.18%) in Experimental Unit 1 were both higher than those in Control Unit 6 (0.76g, 0.16%). At 21 days of age, the bursa-to-body ratio in Experimental Unit 1 (0.11%) was lower than that in the control group, but the weight of Experimental Unit 5 (1.76g) was higher than that in Control Units 4 (1.64g) and 6 (1.46g). At 28 days of age, the differences in weight and bursa-to-body ratio among the groups narrowed, and overall, the bursa of Fabricius development in the experimental groups was better than that in the control group. The experimental results indicate that the combined use of vitanyl oligosaccharide and bioactive peptides promotes the development of the bursa of Fabricius in broilers.

[0042] Table 3: Bursa of Fabricius Detection Note: No data were obtained for 14-day-old babies in Experiment 5 and Control 4.

[0043] (2) Spleen development The spleen is the largest peripheral immune organ in poultry, participating in humoral and cellular immunity. The spleen index (spleen weight to body weight ratio) is an important indicator for evaluating immune function. Data showed that at 14 days of age, the spleen weight (0.47g vs 0.45g) and spleen index (0.09% vs 0.09%) of Experimental Group 1 and Control Group 6 were basically the same. At 21 days of age, the spleen weight of Experimental Group 1 (1.56g) was higher than that of the control group, and the spleen index (0.13%) was the same as that of Control Group 4 and higher than that of Control Group 6. At 28 days of age, the control group had a slightly higher weight, but the experimental group showed better overall spleen index performance. The experimental results indicate that the combined use of vitanyl oligosaccharide and bioactive peptides has a positive promoting effect on spleen development in broilers.

[0044] Table 4: Spleen Development Detection Note: No data were obtained for 14-day-old babies in Experiment 5 and Control 4.

[0045] (3) Intestinal development The intestine is not only an organ for nutrient absorption but also an important immune barrier, and its length reflects its developmental status. Data showed that at 14 days of age, the intestinal length of the control group was slightly higher than that of the experimental group; at 21 days of age, the intestinal length of experimental group 1 (179.6 cm) was significantly higher than that of control group 4 (169.8 cm), control group 6 (168.4 cm), and experimental group 5 (159.4 cm); at 28 days of age, experimental group 1 (207.2 cm) still had the highest intestinal length. Overall, the intestinal length of the experimental group was better than that of the control group, indicating that the combined use of vitanyl oligosaccharide and bioactive peptides has a significant promoting effect on the intestinal development of broilers in the mid-to-late stages.

[0046] Table 5: Intestinal Length Detection Example 4: Statistics on Slaughter Data The final output data directly reflects the economic benefits of this invention. Based on Example 3, this experiment recorded key indicators for both groups of broilers, including feeding days, number of chickens at slaughter, survival rate, slaughter weight, feed conversion ratio, European index, and medication costs. Specific data are shown in Table 6 below. Table 6: Scale Output Data The experimental group outperformed the control group in all aspects, including survival rate, body weight, feed efficiency, and overall benefit index.

[0047] To ensure fairness in profit calculation, the survival rate of the experimental group was adjusted to be consistent with that of the control group. Simultaneously, the market weight was reduced and the purchase price was leveled out. The benefits were compared under the same benchmark, and the calculation results are shown in Table 7. Table 7: Profit Calculation The control group paid 0.64 yuan per bird in 4 broilers and 0.80 yuan per bird in 6 broilers, averaging 0.72 yuan per bird. The experimental group paid 0.25 yuan per bird in 1 broiler and 0.84 yuan per bird in 5 broilers, averaging 0.55 yuan per bird. The experimental group paid 0.17 yuan less per bird than the control group. Considering the cost of experimental drugs (averaging 0.08 yuan per bird), the experimental group ultimately achieved a net profit of 0.09 yuan more per broiler compared to the control group, demonstrating a significant improvement in farming efficiency.

[0048] Example 5: Detection of ND antibody levels To verify the synergistic effect of combining vitanulinosaccharides and bioactive peptides, experimental groups 1, 2, 3, and 4 were set up. Each group contained 20 one-day-old chicks. The management measures were the same for all groups, and the specific feeding details are as follows: Experimental Group 1: 15g of the composition prepared in Example 1 was weighed and dissolved in 250mL of water to obtain a composition solution. Experimental Group 1 chickens were fed this solution continuously for 14 days starting from day 1 of age, at a dosage of 20 chickens / 0.25mL. Experimental Group 2: 15g of vitanyl oligosaccharide was dissolved in 250mL of water to obtain a vitanyl oligosaccharide solution. Experimental Group 2 broilers were fed this solution for 14 consecutive days starting from day 1 of age, at a dosage of 20 birds / 0.25mL. Experimental Group 3: 15g of bioactive peptide (prepared using the same method as in Example 1) was dissolved in 250mL of water to obtain a bioactive peptide solution. Experimental Group 3 broilers were fed this solution for 14 consecutive days starting from day 1 of age, at a dose of 20 birds / 0.25mL. Experimental group 4 served as the control group, and was fed the same amount of water during this period.

[0049] Red blood cells were collected from chicks at 14, 21, and 28 days of age to extract serum and detect ND antibody levels. ND antibody (Newcastle disease antibody) levels are an important indicator for assessing the humoral immune function of broilers. The antibody level data for the 14-day, 21-day, and 28-day-old flocks are shown in Table 8.

[0050] Table 8: Detection of ND antibody levels Experimental results show that combining vitanol oligosaccharides with bioactive peptides achieves a synergistic effect of "1+1>2" in improving ND antibody levels. This composition can synergistically, efficiently, and persistently stimulate and maintain specific immunity against Newcastle disease in broilers, providing an innovative solution to problems such as low antibody levels, short duration of immunity, and poor population uniformity in broiler farming.

[0051] The above description is merely a preferred embodiment of this application and is not intended to limit the application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications made within the spirit and principles of this application are not permitted. Equivalent substitutions and improvements should all be included within the scope of protection of this application.

Claims

1. A composition for enhancing the immunity of poultry, characterized in that, The mass ratio of vitanol oligosaccharide to bioactive peptide in the composition is 1:(1-2).

2. The composition according to claim 1, characterized in that, The mass ratio of vitanol oligosaccharide and bioactive peptide in the composition is 1:

1.

3. The composition according to claim 1 or 2, characterized in that, The preparation method of the bioactive peptide includes the following steps: (1) Weigh out Astragalus membranaceus, Polygonatum sibiricum and glycosides, mix them evenly, add distilled water and extract in a water bath at 90-95℃, and collect the filtrate; (2) Add liquid excipients to the filtrate, adjust the pH to 5.5-6.5, autoclave, and obtain liquid culture medium; (3) Inoculate the Bacillus subtilis culture solution into the liquid culture medium at a volume ratio of 5%-10% and ferment to obtain bioactive peptides.

4. Use of the composition of claim 1 in any one of (1)-(4): (1) Preparation of feed additives for improving ND antibody levels in poultry; (2) Prepare feed additives for promoting the development of poultry immune organs; (3) Prepare feed additives to improve the survival rate of poultry; (4) Prepare feed additives for improving the feed conversion ratio and increasing the slaughter weight of poultry.

5. The application according to claim 4, characterized in that, The poultry mentioned is broiler chicken.

6. The application according to claim 4, characterized in that, The feed additive may also contain feed-grade acceptable carriers or excipients.

7. The application according to claim 6, characterized in that, The feed-grade acceptable carrier is selected from one or more of wheat bran, rice husk powder, zeolite powder, silicon dioxide, and calcium carbonate.

8. The application according to claim 4, characterized in that, The feed additive is in the form of powder, granules, or liquid.

9. A method for improving the immunity and growth performance of chicks, characterized in that, Includes the following steps: Weigh 15-20g of the composition according to claim 1, dissolve it in water to obtain a composition solution, and feed the chicks the composition solution continuously for 10-14 days during the growth stage from hatching to 3 weeks of age by drinking water.

10. The method according to claim 9, characterized in that, During the period from hatching to 3 weeks of age, the composition is fed at a daily dose of (0.01-0.015 mL) per chicken.

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